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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK393 (mAID-mCherry2-BSD)

A plasmid for construction of C-terminal mAID-mCherry2-BSD tagging donors.

Catalog number RDB16821
Resource name pMK393 (mAID-mCherry2-BSD)
Clone info. A plasmid for construction of C-terminal mAID-mCherry2-BSD tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Discosoma sp. RFP cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank

External Database
Arabidopsis thaliana AtIAA17 Discosoma sp. RFP

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
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          RDB16821 pMK393 (mAID-mCherry2-BSD) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pMK393 (mAID-mCherry2-BSD) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16821).

          Reference section:

          Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          Tips

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16821_A8Lpp1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pUC-M13_-100 (Pr0310)
          Region: T7 pro,insert 5'(Linker,mAID,mCherry2 5')
          Sequence file: RDB16821_A8Lpe.seq check
          >D06157B8_A8Lp_1_pUC-M13_-100_A01_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN TTGGGNNNGC NNGGGTTTTC CCNGTCACGA CGTTGTAAAA
             61 CGACGGCCAG TGAGCGCGCG TAATACGACT CACTATAGGG CGAATTGGAG CTCCCCGGGA
            121 TCCGGTGCAG GCGCCAAGGA GAAGAGTGCT TGTCCTAAAG ATCCAGCCAA ACCTCCGGCC
            181 AAGGCACAAG TTGTGGGATG GCCACCGGTG AGATCATACC GGAAGAACGT GATGGTTTCC
            241 TGCCAAAAAT CAAGCGGTGG CCCGGAGGCG GCGGCGTTCG TGAAGGTATC AATGGACGGA
            301 GCACCGTACT TGAGGAAAAT CGATTTGAGG ATGTATAAAG CTAGCATGGT GAGCAAGGGC
            361 GAGGAGGATA ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA CATGGAGGGC
            421 TCCGTGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC
            481 ACCCAGACCG CCAAGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC CTGGGACATC
            541 CTGTCCCCTC AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC CGACATCCCC
            601 GACTACTTGA AGCTGTCCTT CCCCGAGGGC TTCAATTGGG AGCGCGTGAT GAACTTCGAG
            661 GACGNNNNNN TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA GTTCATCTAC
            721 AAGGTGAAGC TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA GTGTCGTACC
            781 ATGGGCTGGG AGGCCTCCAC TGAGCGGATG TACCCCGAGG ANGGCGCCCT GAANGGNGAG
            841 ATCAAGCAGA NGCTGAAGCT GAAGGACGGC GNCCACTACN ANGCTGANGN CAAGANCACC
            901 TACAAGGNCN AGAAGCCCGT GCAGCTGCCC GGCGCNTNNN NCNNCNANNT CNAGTNGNAN
            961 NTNCTTTNCC NCNNCNAGNN NNACACNNTC NTGNAACAGT ACNAANNNGN CNAGGNNCGC
           1021 NNNTNNNNNN NNNGCNTNNN NNANCTGNNN NNGTANTCTN NANNNNGATC NTAATCNNNN
          1081 NNNNNN
          //
          Primer: reverse (Pr0099)
          Region: insert 3'(loxP.2,mPGK ter,BSD 3')
          Sequence file: RDB16821_A8Lpf.seq check
          >D06157B8_A8Lp_1_reverse_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNAAGGGNNN NAAAGCTGGG TACCGGATCC TAGTGAACCT
             61 CTTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC GAAGTTATAT TAAGGGTTCC
            121 GGATCTCGAC CAGCTTCTGA TGGAATTAGA ACTTGGCAAA ACAATACTGA GAATGAAGTG
            181 TATGTGGAAC AGAGGCTGCT GATCTCGTTC TTCAGGCTAT GAAACTGACA CATTTGGAAA
            241 CCACAGTACT TAGAACCACA AAGTGGGAAT CAAGAGAAAA ACAATGATCC CACGAGAGAT
            301 CTATAGATCT ATAGATCATG AGTGGGAGGA ATGAGCTGGC CCTTAATTTG GTTTTGCTTG
            361 TTTAAATTAT GATATCCAAC TATGAAACAT TATCATAAAG CAATAGTAAA GAGCCTTCAG
            421 TAAAGAGCAG GCATTTATCT AATCCCACCC CACCCCCACC CCCGTAGCTC CAATCCTTCC
            481 ATTCAAAATG TAGGTACTCT GTTCTCACCC TTCTTAACAA AGTATGACAG GAAAAACTTC
            541 CATTTTAGTG GACATCTTTA TTGTTTAATA GATCATCAAT TTCTGCATCC CGGGGATCTG
            601 ATATCATCGA TTAGCCCTCC CACACATAAC CAGAGGGCAG CAATTCACGA ATCCCAACTG
            661 CCGTCGGCTG TCCATCACTG TCCTTCACTA TGGCTTTGAT CCCAGGATGC AGATCGAGAA
            721 GCACCTGTCG GCACCGTCCG CAGGGGCTCA AGATGCCCCT GTTCTCATTT CCGATCGCGA
            781 CGATACAAGT CAGGTTGCCN GCTGCCGCAG CAGCAGCAGT GCCCAGCACC ACGAGTTCTG
            841 CACAANGTCC CCCAGTAAAA TGATATACAT TGACACCNGT GANNANGCGN NCGTCGCTNG
            901 AGAGAGCTGN NNTGGNGANN CTGTANNCTT CNGAGANGGG GANGNTGNNG ATTGNAGNCG
          961 NTGNTCTTNN NNNGNNGNNG GANTCTNCNN GANANNAAGG NTTNGNNNNN NNNNN
          //
          Primer: PGK_N_rev (Pr0243)
          Region: insert mid(loxP.1,SV40 pA,mCherry2 3')
          Sequence file: RDB16821_A8Lpg.seq check
          >D06157B8_A8Lp_1_PGK_N_rev_C01_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN CNGGNAGANN TTCGAGGTCG AGNNCCTAAG CTTGGCTGGA
             61 CGTAAACTCC TCTTCAGACC TAATAACTTC GTATAGCATA CATTATACGA AGTTATATTA
            121 AGGGTTATTG AATATGATCG ACGCGTTAAG ATACATTGAT GAGTTTGGAC AAACCACAAC
            181 TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT
            241 AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA
            301 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT
            361 GGCTGATTAT GATCAGTTAT CTAGATTACT TGTACAGCTC GTCCATGCCG CCGGTGGAGT
            421 GGCGGCCCTC GGCGCGTTCG TACTGTTCCA CGATGGTGTA GTCCTCGTTG TGGGAAAGGA
            481 TGTCCAACTT GATGTCGACG TTGTAGGCGC CGGGCAGCTG CACGGGCTTC TTGGCCTTGT
            541 AGGTGGTCTT GACCTCAGCG TCGTAGTGGC CGCCGTCCTT CAGCTTCAGC CTCTGCTTGA
            601 TCTCGCCCTT CAGGGCGCCG TCCTCGGGGT ACATCCGCTC AGTGGAGGCC TCCCAGCCCA
            661 TGGTACGACA CTGCATTACG GGGCCGTCGG AGGGGAAGTT GGTGCCGCGC AGCTTCACCT
            721 TGTAGATGAA CTCGCCGTCC TGCAGGGAGG AGTCCTGGGT CACGGTCACC ACGCCGCCGT
            781 CCTCGAAGTT CATCACGCGC TCCCAATTGA AGCCCTCGGG GAAGGACAGC TTCAAGTAGT
            841 CGGGGANGTC GNCGGGGTGC TTCACGTANG CCTTGNAGCC GTACATGANN TGANGGGANA
            901 GGANGTCCCN NGCGAANGNN ANGGGGNCNC CCNNGGTCAC CNNCAGCTNG GNGGNCTGGG
            961 TGNCNTNNTA NGGGNGGNCC TCGNNNNNGN CCNNNATNTN NAANTNGTGG NNGTNNNNNN
           1021 ANNNNTNNNT GTGCACCNTN NNNNNNNTGN NNNNCNNNNN GANNNNNANN NNATNNNNNN
          1081 NNNN
          //
          Primer: PGKpro_F (Pr0249)
          Region: insert mid(BSD,mPGK ter)
          Sequence file: RDB16821_A8Lph.seq check
          >D06157B8_A8Lp_1_PGKpro_F_F01_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTNN NNNNGCCTTT CGACCTGCAT CCNTCTAGAT
             61 CTCGAGCCAC CATGGCCAAG CCTTTGTCTC AAGAAGAATC CACCCTCATT GAAAGAGCAA
            121 CGGCTACAAT CAACAGCATC CCCATCTCTG AAGACTACAG CGTCGCCAGC GCAGCTCTCT
            181 CTAGCGACGG CCGCATCTTC ACTGGTGTCA ATGTATATCA TTTTACTGGG GGACCTTGTG
            241 CAGAACTCGT GGTGCTGGGC ACTGCTGCTG CTGCGGCAGC TGGCAACCTG ACTTGTATCG
            301 TCGCGATCGG AAATGAGAAC AGGGGCATCT TGAGCCCCTG CGGACGGTGC CGACAGGTGC
            361 TTCTCGATCT GCATCCTGGG ATCAAAGCCA TAGTGAAGGA CAGTGATGGA CAGCCGACGG
            421 CAGTTGGGAT TCGTGAATTG CTGCCCTCTG GTTATGTGTG GGAGGGCTAA TCGATGATAT
            481 CAGATCCCCG GGATGCAGAA ATTGATGATC TATTAAACAA TAAAGATGTC CACTAAAATG
            541 GAAGTTTTTC CTGTCATACT TTGTTAAGAA GGGTGAGAAC AGAGTACCTA CATTTTGAAT
            601 GGAAGGATTG GAGCTACGGG GGTGGGGGTG GGGTGGGATT AGATAANNGN NNGCTCTTNN
            661 NNGAAGGCTC TTTACTATTG CNNNNNGATA ATGTTTCATA GTTGGATATC NNNNNTTAAN
          721 NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

          2023.12.13

          GNP_filter3_RDBDEP_html_231213.pl