Sequencing and PCR primers for primer information.
A plasmid for construction of C-terminal mAID-mCherry2-BSD tagging donors.
| Catalog number | RDB16821 |
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| Resource name | pMK393 (mAID-mCherry2-BSD) |
| Clone info. | A plasmid for construction of C-terminal mAID-mCherry2-BSD tagging donors. |
| Vector backbone | pBluescript (plasmid) |
| Size of vector backbone | 2.9 kb |
| Selectable markers | Am^r (E. coli) |
| Gene/insert name | Arabidopsis thaliana AtIAA17 cDNA Discosoma sp. RFP cDNA |
| Depositor | Kanemaki, Masato | |
| Other clones in our bank | Arabidopsis thaliana AXR3 (KEGG orthology K14484) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Exclusive MTA [Link] |
| Remarks | The BIOLOGICAL RESOURCE may not be used for COMMERCIAL PURPOSES. Please read the Exclusive MTA (Clontech FPs). |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB16821 | pMK393 (mAID-mCherry2-BSD) | DNA solution |
Electronic file
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Auxin Inducible Degron (AID) System (English text) |
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| Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB16821_A8Lpp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pUC-M13_-100 | RDB16821_A8Lpa.seq |
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>D06157B8_A8Lp_1_pUC-M13_-100_A01_01_ABI08.ab1 1 GGTTGGCAAG GCGGAATTAG TTGGGTACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA 61 CGACGGCCAG TGAGCGCGCG TAATACGACT CACTATAGGG CGAATTGGAG CTCCCCGGGA 121 TCCGGTGCAG GCGCCAAGGA GAAGAGTGCT TGTCCTAAAG ATCCAGCCAA ACCTCCGGCC 181 AAGGCACAAG TTGTGGGATG GCCACCGGTG AGATCATACC GGAAGAACGT GATGGTTTCC 241 TGCCAAAAAT CAAGCGGTGG CCCGGAGGCG GCGGCGTTCG TGAAGGTATC AATGGACGGA 301 GCACCGTACT TGAGGAAAAT CGATTTGAGG ATGTATAAAG CTAGCATGGT GAGCAAGGGC 361 GAGGAGGATA ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA CATGGAGGGC 421 TCCGTGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC 481 ACCCAGACCG CCAAGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC CTGGGACATC 541 CTGTCCCCTC AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC CGACATCCCC 601 GACTACTTGA AGCTGTCCTT CCCCGAGGGC TTCAATTGGG AGCGCGTGAT GAACTTCGAG 661 GACGGCTGCG TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA GTTCATCTAC 721 AAGGTGAAGC TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA GTGTCGTACC 781 ATGGGCTGGG AGGCCTCCAC TGAGCGGATG TACCCCGAGG ACGGCGCCCT GAAGGGCGAG 841 ATCAAGCAGA GGCTGAAGCT GAAGGACGGC GGCCACTACG ACGCTGAGGT CAAGACCACC 901 TACAAGGCCA AGAAGCCCGT GCAGCTGCCC GGCGCCTACA ACGTCGACAT CAAGTTGGAC 961 ATCCTTTCCC ACAACGAGGA CTACACCATC GTGGAACAGT ACGAACGCGC CGAGGGCCGC 1021 CACTCCACCG GCGGCATGGA CGAGCTGTAC AAGTAATCTA GATACTGATC ATAATCAGCC 1081 ATACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACTC CCCACACCTC CCCTGAACTG 1141 AACAATAAAT GATGCATGTG GTGGTACTTG TATGCAGCTA TATGGTTACA ATTAAGCATT 1201 AGCATTCCCC AAAAT // |
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| Primer: reverse | RDB16821_A8Lpb.seq |
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>D06157B8_A8Lp_1_reverse_B01_02_ABI08.ab1 1 ACCCGCAAAT TAAACCTCAC TAAAGGGAAC AAAAGCTGGG TACCGGATCC TAGTGAACCT 61 CTTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC GAAGTTATAT TAAGGGTTCC 121 GGATCTCGAC CAGCTTCTGA TGGAATTAGA ACTTGGCAAA ACAATACTGA GAATGAAGTG 181 TATGTGGAAC AGAGGCTGCT GATCTCGTTC TTCAGGCTAT GAAACTGACA CATTTGGAAA 241 CCACAGTACT TAGAACCACA AAGTGGGAAT CAAGAGAAAA ACAATGATCC CACGAGAGAT 301 CTATAGATCT ATAGATCATG AGTGGGAGGA ATGAGCTGGC CCTTAATTTG GTTTTGCTTG 361 TTTAAATTAT GATATCCAAC TATGAAACAT TATCATAAAG CAATAGTAAA GAGCCTTCAG 421 TAAAGAGCAG GCATTTATCT AATCCCACCC CACCCCCACC CCCGTAGCTC CAATCCTTCC 481 ATTCAAAATG TAGGTACTCT GTTCTCACCC TTCTTAACAA AGTATGACAG GAAAAACTTC 541 CATTTTAGTG GACATCTTTA TTGTTTAATA GATCATCAAT TTCTGCATCC CGGGGATCTG 601 ATATCATCGA TTAGCCCTCC CACACATAAC CAGAGGGCAG CAATTCACGA ATCCCAACTG 661 CCGTCGGCTG TCCATCACTG TCCTTCACTA TGGCTTTGAT CCCAGGATGC AGATCGAGAA 721 GCACCTGTCG GCACCGTCCG CAGGGGCTCA AGATGCCCCT GTTCTCATTT CCGATCGCGA 781 CGATACAAGT CAGGTTGCCA GCTGCCGCAG CAGCAGCAGT GCCCAGCACC ACGAGTTCTG 841 CACAAGGTCC CCCAGTAAAA TGATATACAT TGACACCAGT GAAGATGCGG CCGTCGCTAG 901 AGAGAGCTGC GCTGGCGACG CTGTAGTCTT CAGAGATGGG GATGCTGTTG ATTGTAGCCG 961 TTGCTCTTTC AATGAGGGTG GATTCTTCTT GAGACAAAGG CTTGGGCCAT GGTGGCTCGA 1021 GATCTAGATG GATGCAGGTC GAAAGGCCCG GAGATGAGAA GAGGAGACAG CGCGGCAGAC 1081 GTGCGCTTTT GAGCGGTGCA GATGCCGGGC CTCCGGAGGA CTTCGGGCGC GCCCGCCCTG 1141 AGCCGGCCTG AGCCGGCCCC GGACCACCTC CAGCTCTGAG CAGAAGCGCA GGAGCAAGGC 1201 TCGTGCTAA // |
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| Primer: PGK_N_rev | RDB16821_A8Lpc.seq |
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>D06157B8_A8Lp_1_PGK_N_rev_C01_03_ABI08.ab1 1 CGGTTGAAGC GCCTCCCTAC CCGGTAGAAT TTCGAGGTCG AGATCCTAAG CTTGGCTGGA 61 CGTAAACTCC TCTTCAGACC TAATAACTTC GTATAGCATA CATTATACGA AGTTATATTA 121 AGGGTTATTG AATATGATCG ACGCGTTAAG ATACATTGAT GAGTTTGGAC AAACCACAAC 181 TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT 241 AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA 301 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT 361 GGCTGATTAT GATCAGTTAT CTAGATTACT TGTACAGCTC GTCCATGCCG CCGGTGGAGT 421 GGCGGCCCTC GGCGCGTTCG TACTGTTCCA CGATGGTGTA GTCCTCGTTG TGGGAAAGGA 481 TGTCCAACTT GATGTCGACG TTGTAGGCGC CGGGCAGCTG CACGGGCTTC TTGGCCTTGT 541 AGGTGGTCTT GACCTCAGCG TCGTAGTGGC CGCCGTCCTT CAGCTTCAGC CTCTGCTTGA 601 TCTCGCCCTT CAGGGCGCCG TCCTCGGGGT ACATCCGCTC AGTGGAGGCC TCCCAGCCCA 661 TGGTACGACA CTGCATTACG GGGCCGTCGG AGGGGAAGTT GGTGCCGCGC AGCTTCACCT 721 TGTAGATGAA CTCGCCGTCC TGCAGGGAGG AGTCCTGGGT CACGGTCACC ACGCCGCCGT 781 CCTCGAAGTT CATCACGCGC TCCCAATTGA AGCCCTCGGG GAAGGACAGC TTCAAGTAGT 841 CGGGGATGTC GGCGGGGTGC TTCACGTAGG CCTTGGAGCC GTACATGAAC TGAGGGGACA 901 GGATGTCCCA GGCGAAGGGC AGGGGGCCAC CCTTGGTCAC CTTCAGCTTG GCGGTCTGGG 961 TGCCCTCGTA GGGGCGGCCC TCGCCCTCGC CCTCGATCTC GAACTCGTGG CCGTTCACGG 1021 AGCCCTCCAT GTGCACCTTG AAGCGCATGA ACTCCTTGAT GATGGCCATG TTATCCTCCT 1081 CGCCTGCTCA CATGCTAGCT TTATACATCC TCAAATCGAT TTTCTCAGTA CGGTGCTCGG 1141 TTCCATTGAA TACGTCACGA CGCCGCCGGC CTCGGCAACG GCTTGGATTT GGGCAGGATC 1201 ATACGTCTGC CGGATTGAAA TCTTACATCT TGTAAGT // |
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| Primer: PGKpro_F | RDB16821_A8Lpd.seq |
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> GGCGGCGGCCGGTTTCTCCTCTTCTCATCTCCGGGCCTTTCGACCTGCATCCATCTAGATCTCGAGCCACCATGGCCAAG 1 CCTTTGTCTC AAGAAGAATC CACCCTCATT GAAAGAGCAA CGGCTACAAT CAACAGCATC 61 CCCATCTCTG AAGACTACAG CGTCGCCAGC GCAGCTCTCT CTAGCGACGG CCGCATCTTC 121 ACTGGTGTCA ATGTATATCA TTTTACTGGG GGACCTTGTG CAGAACTCGT GGTGCTGGGC 181 ACTGCTGCTG CTGCGGCAGC TGGCAACCTG ACTTGTATCG TCGCGATCGG AAATGAGAAC 241 AGGGGCATCT TGAGCCCCTG CGGACGGTGC CGACAGGTGC TTCTCGATCT GCATCCTGGG 301 ATCAAAGCCA TAGTGAAGGA CAGTGATGGA CAGCCGACGG CAGTTGGGAT TCGTGAATTG 361 CTGCCCTCTG GTTATGTGTG GGAGGGCTAA TCGATGATAT CAGATCCCCG GGATGCAGAA 421 ATTGATGATC TATTAAACAA TAAAGATGTC CACTAAAATG GAAGTTTTTC CTGTCATACT 481 TTGTTAAGAA GGGTGAGAAC AGAGTACCTA CATTTTGAAT GGAAGGATTG GAGCTACGGG 541 GGTGGGGGTG GGGTGGGATT A // |
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Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
2019.07.12
GNP_filter3_RDBDEP_html_190431.pl