RDB16821 - pMK393 (mAID-mCherry2-BSD)

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
Remarks	This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. A part of license fees is required. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内)

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
備考	Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。ライセンス料相当額を負担して頂きます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

References and tips

QC test results

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB16821	pMK393 (mAID-mCherry2-BSD)	DNA solution

original	Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

Featured content	Auxin Inducible Degron (AID) System (English text)
Featured content	Auxin Inducible Degron (AID) System (Japanese text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: pUC-M13_-100 Region: T7 pro,insert 5'(Linker,mAID,mCherry2 5') Sequence file: RDB16821_A8Lpe.seq
>D06157B8_A8Lp_1_pUC-M13_-100_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN TTGGGNNNGC NNGGGTTTTC CCNGTCACGA CGTTGTAAAA 61 CGACGGCCAG TGAGCGCGCG TAATACGACT CACTATAGGG CGAATTGGAG CTCCCCGGGA 121 TCCGGTGCAG GCGCCAAGGA GAAGAGTGCT TGTCCTAAAG ATCCAGCCAA ACCTCCGGCC 181 AAGGCACAAG TTGTGGGATG GCCACCGGTG AGATCATACC GGAAGAACGT GATGGTTTCC 241 TGCCAAAAAT CAAGCGGTGG CCCGGAGGCG GCGGCGTTCG TGAAGGTATC AATGGACGGA 301 GCACCGTACT TGAGGAAAAT CGATTTGAGG ATGTATAAAG CTAGCATGGT GAGCAAGGGC 361 GAGGAGGATA ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA CATGGAGGGC 421 TCCGTGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC 481 ACCCAGACCG CCAAGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC CTGGGACATC 541 CTGTCCCCTC AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC CGACATCCCC 601 GACTACTTGA AGCTGTCCTT CCCCGAGGGC TTCAATTGGG AGCGCGTGAT GAACTTCGAG 661 GACGNNNNNN TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA GTTCATCTAC 721 AAGGTGAAGC TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA GTGTCGTACC 781 ATGGGCTGGG AGGCCTCCAC TGAGCGGATG TACCCCGAGG ANGGCGCCCT GAANGGNGAG 841 ATCAAGCAGA NGCTGAAGCT GAAGGACGGC GNCCACTACN ANGCTGANGN CAAGANCACC 901 TACAAGGNCN AGAAGCCCGT GCAGCTGCCC GGCGCNTNNN NCNNCNANNT CNAGTNGNAN 961 NTNCTTTNCC NCNNCNAGNN NNACACNNTC NTGNAACAGT ACNAANNNGN CNAGGNNCGC 1021 NNNTNNNNNN NNNGCNTNNN NNANCTGNNN NNGTANTCTN NANNNNGATC NTAATCNNNN 1081 NNNNNN //
Primer: reverse Region: insert 3'(loxP.2,mPGK ter,BSD 3') Sequence file: RDB16821_A8Lpf.seq
>D06157B8_A8Lp_1_reverse_B01_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNAAGGGNNN NAAAGCTGGG TACCGGATCC TAGTGAACCT 61 CTTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC GAAGTTATAT TAAGGGTTCC 121 GGATCTCGAC CAGCTTCTGA TGGAATTAGA ACTTGGCAAA ACAATACTGA GAATGAAGTG 181 TATGTGGAAC AGAGGCTGCT GATCTCGTTC TTCAGGCTAT GAAACTGACA CATTTGGAAA 241 CCACAGTACT TAGAACCACA AAGTGGGAAT CAAGAGAAAA ACAATGATCC CACGAGAGAT 301 CTATAGATCT ATAGATCATG AGTGGGAGGA ATGAGCTGGC CCTTAATTTG GTTTTGCTTG 361 TTTAAATTAT GATATCCAAC TATGAAACAT TATCATAAAG CAATAGTAAA GAGCCTTCAG 421 TAAAGAGCAG GCATTTATCT AATCCCACCC CACCCCCACC CCCGTAGCTC CAATCCTTCC 481 ATTCAAAATG TAGGTACTCT GTTCTCACCC TTCTTAACAA AGTATGACAG GAAAAACTTC 541 CATTTTAGTG GACATCTTTA TTGTTTAATA GATCATCAAT TTCTGCATCC CGGGGATCTG 601 ATATCATCGA TTAGCCCTCC CACACATAAC CAGAGGGCAG CAATTCACGA ATCCCAACTG 661 CCGTCGGCTG TCCATCACTG TCCTTCACTA TGGCTTTGAT CCCAGGATGC AGATCGAGAA 721 GCACCTGTCG GCACCGTCCG CAGGGGCTCA AGATGCCCCT GTTCTCATTT CCGATCGCGA 781 CGATACAAGT CAGGTTGCCN GCTGCCGCAG CAGCAGCAGT GCCCAGCACC ACGAGTTCTG 841 CACAANGTCC CCCAGTAAAA TGATATACAT TGACACCNGT GANNANGCGN NCGTCGCTNG 901 AGAGAGCTGN NNTGGNGANN CTGTANNCTT CNGAGANGGG GANGNTGNNG ATTGNAGNCG 961 NTGNTCTTNN NNNGNNGNNG GANTCTNCNN GANANNAAGG NTTNGNNNNN NNNNN //
Primer: PGK_N_rev Region: insert mid(loxP.1,SV40 pA,mCherry2 3') Sequence file: RDB16821_A8Lpg.seq
>D06157B8_A8Lp_1_PGK_N_rev_C01_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN CNGGNAGANN TTCGAGGTCG AGNNCCTAAG CTTGGCTGGA 61 CGTAAACTCC TCTTCAGACC TAATAACTTC GTATAGCATA CATTATACGA AGTTATATTA 121 AGGGTTATTG AATATGATCG ACGCGTTAAG ATACATTGAT GAGTTTGGAC AAACCACAAC 181 TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT 241 AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA 301 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT 361 GGCTGATTAT GATCAGTTAT CTAGATTACT TGTACAGCTC GTCCATGCCG CCGGTGGAGT 421 GGCGGCCCTC GGCGCGTTCG TACTGTTCCA CGATGGTGTA GTCCTCGTTG TGGGAAAGGA 481 TGTCCAACTT GATGTCGACG TTGTAGGCGC CGGGCAGCTG CACGGGCTTC TTGGCCTTGT 541 AGGTGGTCTT GACCTCAGCG TCGTAGTGGC CGCCGTCCTT CAGCTTCAGC CTCTGCTTGA 601 TCTCGCCCTT CAGGGCGCCG TCCTCGGGGT ACATCCGCTC AGTGGAGGCC TCCCAGCCCA 661 TGGTACGACA CTGCATTACG GGGCCGTCGG AGGGGAAGTT GGTGCCGCGC AGCTTCACCT 721 TGTAGATGAA CTCGCCGTCC TGCAGGGAGG AGTCCTGGGT CACGGTCACC ACGCCGCCGT 781 CCTCGAAGTT CATCACGCGC TCCCAATTGA AGCCCTCGGG GAAGGACAGC TTCAAGTAGT 841 CGGGGANGTC GNCGGGGTGC TTCACGTANG CCTTGNAGCC GTACATGANN TGANGGGANA 901 GGANGTCCCN NGCGAANGNN ANGGGGNCNC CCNNGGTCAC CNNCAGCTNG GNGGNCTGGG 961 TGNCNTNNTA NGGGNGGNCC TCGNNNNNGN CCNNNATNTN NAANTNGTGG NNGTNNNNNN 1021 ANNNNTNNNT GTGCACCNTN NNNNNNNTGN NNNNCNNNNN GANNNNNANN NNATNNNNNN 1081 NNNN //
Primer: PGKpro_F Region: insert mid(BSD,mPGK ter) Sequence file: RDB16821_A8Lph.seq
>D06157B8_A8Lp_1_PGKpro_F_F01_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTNN NNNNGCCTTT CGACCTGCAT CCNTCTAGAT 61 CTCGAGCCAC CATGGCCAAG CCTTTGTCTC AAGAAGAATC CACCCTCATT GAAAGAGCAA 121 CGGCTACAAT CAACAGCATC CCCATCTCTG AAGACTACAG CGTCGCCAGC GCAGCTCTCT 181 CTAGCGACGG CCGCATCTTC ACTGGTGTCA ATGTATATCA TTTTACTGGG GGACCTTGTG 241 CAGAACTCGT GGTGCTGGGC ACTGCTGCTG CTGCGGCAGC TGGCAACCTG ACTTGTATCG 301 TCGCGATCGG AAATGAGAAC AGGGGCATCT TGAGCCCCTG CGGACGGTGC CGACAGGTGC 361 TTCTCGATCT GCATCCTGGG ATCAAAGCCA TAGTGAAGGA CAGTGATGGA CAGCCGACGG 421 CAGTTGGGAT TCGTGAATTG CTGCCCTCTG GTTATGTGTG GGAGGGCTAA TCGATGATAT 481 CAGATCCCCG GGATGCAGAA ATTGATGATC TATTAAACAA TAAAGATGTC CACTAAAATG 541 GAAGTTTTTC CTGTCATACT TTGTTAAGAA GGGTGAGAAC AGAGTACCTA CATTTTGAAT 601 GGAAGGATTG GAGCTACGGG GGTGGGGGTG GGGTGGGATT AGATAANNGN NNGCTCTTNN 661 NNGAAGGCTC TTTACTATTG CNNNNNGATA ATGTTTCATA GTTGGATATC NNNNNTTAAN 721 NNNNNNNNN //

pMK393 (mAID-mCherry2-BSD)

Distribution information

References and tips

QC test results

References