Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK393 (mAID-mCherry2-BSD)

A plasmid for construction of C-terminal mAID-mCherry2-BSD tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose)[Link]
Remarks	The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose).

Distribution information (domestic users) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 専用MTA (Takara Bio USA, Inc. FPs, 英語版のみ、非営利機関による非営利学術研究用)[Link]
備考	本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。専用MTA（Takara Bio USA, Inc. FPs, 英語版のみ、非営利機関による非営利学術研究用）をご参照ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16821_A8Lpp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 Region: Linker, mAID, mCherry2 5' Sequence file: RDB16821_A8Lpa.seq
>D06157B8_A8Lp_1_pUC-M13_-100_A01_01_ABI08.ab1     1 GGTTGGCAAG GCGGAATTAG TTGGGTACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA    61 CGACGGCCAG TGAGCGCGCG TAATACGACT CACTATAGGG CGAATTGGAG CTCCCCGGGA   121 TCCGGTGCAG GCGCCAAGGA GAAGAGTGCT TGTCCTAAAG ATCCAGCCAA ACCTCCGGCC   181 AAGGCACAAG TTGTGGGATG GCCACCGGTG AGATCATACC GGAAGAACGT GATGGTTTCC   241 TGCCAAAAAT CAAGCGGTGG CCCGGAGGCG GCGGCGTTCG TGAAGGTATC AATGGACGGA   301 GCACCGTACT TGAGGAAAAT CGATTTGAGG ATGTATAAAG CTAGCATGGT GAGCAAGGGC   361 GAGGAGGATA ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA CATGGAGGGC   421 TCCGTGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC   481 ACCCAGACCG CCAAGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC CTGGGACATC   541 CTGTCCCCTC AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC CGACATCCCC   601 GACTACTTGA AGCTGTCCTT CCCCGAGGGC TTCAATTGGG AGCGCGTGAT GAACTTCGAG   661 GACGGCTGCG TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA GTTCATCTAC   721 AAGGTGAAGC TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA GTGTCGTACC   781 ATGGGCTGGG AGGCCTCCAC TGAGCGGATG TACCCCGAGG ACGGCGCCCT GAAGGGCGAG   841 ATCAAGCAGA GGCTGAAGCT GAAGGACGGC GGCCACTACG ACGCTGAGGT CAAGACCACC   901 TACAAGGCCA AGAAGCCCGT GCAGCTGCCC GGCGCCTACA ACGTCGACAT CAAGTTGGAC   961 ATCCTTTCCC ACAACGAGGA CTACACCATC GTGGAACAGT ACGAACGCGC CGAGGGCCGC  1021 CACTCCACCG GCGGCATGGA CGAGCTGTAC AAGTAATCTA GATACTGATC ATAATCAGCC  1081 ATACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACTC CCCACACCTC CCCTGAACTG  1141 AACAATAAAT GATGCATGTG GTGGTACTTG TATGCAGCTA TATGGTTACA ATTAAGCATT 1201 AGCATTCCCC AAAAT //
Primer: reverse Region: loxP-2, mouse PGK ter, BSD Sequence file: RDB16821_A8Lpb.seq
>D06157B8_A8Lp_1_reverse_B01_02_ABI08.ab1     1 ACCCGCAAAT TAAACCTCAC TAAAGGGAAC AAAAGCTGGG TACCGGATCC TAGTGAACCT    61 CTTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC GAAGTTATAT TAAGGGTTCC   121 GGATCTCGAC CAGCTTCTGA TGGAATTAGA ACTTGGCAAA ACAATACTGA GAATGAAGTG   181 TATGTGGAAC AGAGGCTGCT GATCTCGTTC TTCAGGCTAT GAAACTGACA CATTTGGAAA   241 CCACAGTACT TAGAACCACA AAGTGGGAAT CAAGAGAAAA ACAATGATCC CACGAGAGAT   301 CTATAGATCT ATAGATCATG AGTGGGAGGA ATGAGCTGGC CCTTAATTTG GTTTTGCTTG   361 TTTAAATTAT GATATCCAAC TATGAAACAT TATCATAAAG CAATAGTAAA GAGCCTTCAG   421 TAAAGAGCAG GCATTTATCT AATCCCACCC CACCCCCACC CCCGTAGCTC CAATCCTTCC   481 ATTCAAAATG TAGGTACTCT GTTCTCACCC TTCTTAACAA AGTATGACAG GAAAAACTTC   541 CATTTTAGTG GACATCTTTA TTGTTTAATA GATCATCAAT TTCTGCATCC CGGGGATCTG   601 ATATCATCGA TTAGCCCTCC CACACATAAC CAGAGGGCAG CAATTCACGA ATCCCAACTG   661 CCGTCGGCTG TCCATCACTG TCCTTCACTA TGGCTTTGAT CCCAGGATGC AGATCGAGAA   721 GCACCTGTCG GCACCGTCCG CAGGGGCTCA AGATGCCCCT GTTCTCATTT CCGATCGCGA   781 CGATACAAGT CAGGTTGCCA GCTGCCGCAG CAGCAGCAGT GCCCAGCACC ACGAGTTCTG   841 CACAAGGTCC CCCAGTAAAA TGATATACAT TGACACCAGT GAAGATGCGG CCGTCGCTAG   901 AGAGAGCTGC GCTGGCGACG CTGTAGTCTT CAGAGATGGG GATGCTGTTG ATTGTAGCCG   961 TTGCTCTTTC AATGAGGGTG GATTCTTCTT GAGACAAAGG CTTGGGCCAT GGTGGCTCGA  1021 GATCTAGATG GATGCAGGTC GAAAGGCCCG GAGATGAGAA GAGGAGACAG CGCGGCAGAC  1081 GTGCGCTTTT GAGCGGTGCA GATGCCGGGC CTCCGGAGGA CTTCGGGCGC GCCCGCCCTG  1141 AGCCGGCCTG AGCCGGCCCC GGACCACCTC CAGCTCTGAG CAGAAGCGCA GGAGCAAGGC 1201 TCGTGCTAA //
Primer: PGK_N_rev Region: PGK pro, loxP-1, SV40p(A), mCherry2 3' Sequence file: RDB16821_A8Lpc.seq
>D06157B8_A8Lp_1_PGK_N_rev_C01_03_ABI08.ab1     1 CGGTTGAAGC GCCTCCCTAC CCGGTAGAAT TTCGAGGTCG AGATCCTAAG CTTGGCTGGA    61 CGTAAACTCC TCTTCAGACC TAATAACTTC GTATAGCATA CATTATACGA AGTTATATTA   121 AGGGTTATTG AATATGATCG ACGCGTTAAG ATACATTGAT GAGTTTGGAC AAACCACAAC   181 TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT   241 AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA   301 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT   361 GGCTGATTAT GATCAGTTAT CTAGATTACT TGTACAGCTC GTCCATGCCG CCGGTGGAGT   421 GGCGGCCCTC GGCGCGTTCG TACTGTTCCA CGATGGTGTA GTCCTCGTTG TGGGAAAGGA   481 TGTCCAACTT GATGTCGACG TTGTAGGCGC CGGGCAGCTG CACGGGCTTC TTGGCCTTGT   541 AGGTGGTCTT GACCTCAGCG TCGTAGTGGC CGCCGTCCTT CAGCTTCAGC CTCTGCTTGA   601 TCTCGCCCTT CAGGGCGCCG TCCTCGGGGT ACATCCGCTC AGTGGAGGCC TCCCAGCCCA   661 TGGTACGACA CTGCATTACG GGGCCGTCGG AGGGGAAGTT GGTGCCGCGC AGCTTCACCT   721 TGTAGATGAA CTCGCCGTCC TGCAGGGAGG AGTCCTGGGT CACGGTCACC ACGCCGCCGT   781 CCTCGAAGTT CATCACGCGC TCCCAATTGA AGCCCTCGGG GAAGGACAGC TTCAAGTAGT   841 CGGGGATGTC GGCGGGGTGC TTCACGTAGG CCTTGGAGCC GTACATGAAC TGAGGGGACA   901 GGATGTCCCA GGCGAAGGGC AGGGGGCCAC CCTTGGTCAC CTTCAGCTTG GCGGTCTGGG   961 TGCCCTCGTA GGGGCGGCCC TCGCCCTCGC CCTCGATCTC GAACTCGTGG CCGTTCACGG  1021 AGCCCTCCAT GTGCACCTTG AAGCGCATGA ACTCCTTGAT GATGGCCATG TTATCCTCCT  1081 CGCCTGCTCA CATGCTAGCT TTATACATCC TCAAATCGAT TTTCTCAGTA CGGTGCTCGG  1141 TTCCATTGAA TACGTCACGA CGCCGCCGGC CTCGGCAACG GCTTGGATTT GGGCAGGATC 1201 ATACGTCTGC CGGATTGAAA TCTTACATCT TGTAAGT //
Primer: PGKpro_F Region: PGK pro, BSD, mouse PGK ter Sequence file: RDB16821_A8Lpd.seq
> GGCGGCGGCCGGTTTCTCCTCTTCTCATCTCCGGGCCTTTCGACCTGCATCCATCTAGATCTCGAGCCACCATGGCCAAG     1 CCTTTGTCTC AAGAAGAATC CACCCTCATT GAAAGAGCAA CGGCTACAAT CAACAGCATC    61 CCCATCTCTG AAGACTACAG CGTCGCCAGC GCAGCTCTCT CTAGCGACGG CCGCATCTTC   121 ACTGGTGTCA ATGTATATCA TTTTACTGGG GGACCTTGTG CAGAACTCGT GGTGCTGGGC   181 ACTGCTGCTG CTGCGGCAGC TGGCAACCTG ACTTGTATCG TCGCGATCGG AAATGAGAAC   241 AGGGGCATCT TGAGCCCCTG CGGACGGTGC CGACAGGTGC TTCTCGATCT GCATCCTGGG   301 ATCAAAGCCA TAGTGAAGGA CAGTGATGGA CAGCCGACGG CAGTTGGGAT TCGTGAATTG   361 CTGCCCTCTG GTTATGTGTG GGAGGGCTAA TCGATGATAT CAGATCCCCG GGATGCAGAA   421 ATTGATGATC TATTAAACAA TAAAGATGTC CACTAAAATG GAAGTTTTTC CTGTCATACT   481 TTGTTAAGAA GGGTGAGAAC AGAGTACCTA CATTTTGAAT GGAAGGATTG GAGCTACGGG 541 GGTGGGGGTG GGGTGGGATT A //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2021.11.02

GNP_filter3_RDBDEP_html_211029.pl