Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK393 (mAID-mCherry2-BSD)

A plasmid for construction of C-terminal mAID-mCherry2-BSD tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	提供依頼書 [Word] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16821_A8Lpp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310) Region: T7 pro,insert 5'(Linker,mAID,mCherry2 5') Sequence file: RDB16821_A8Lpe.seq
>D06157B8_A8Lp_1_pUC-M13_-100_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN TTGGGNNNGC NNGGGTTTTC CCNGTCACGA CGTTGTAAAA    61 CGACGGCCAG TGAGCGCGCG TAATACGACT CACTATAGGG CGAATTGGAG CTCCCCGGGA   121 TCCGGTGCAG GCGCCAAGGA GAAGAGTGCT TGTCCTAAAG ATCCAGCCAA ACCTCCGGCC   181 AAGGCACAAG TTGTGGGATG GCCACCGGTG AGATCATACC GGAAGAACGT GATGGTTTCC   241 TGCCAAAAAT CAAGCGGTGG CCCGGAGGCG GCGGCGTTCG TGAAGGTATC AATGGACGGA   301 GCACCGTACT TGAGGAAAAT CGATTTGAGG ATGTATAAAG CTAGCATGGT GAGCAAGGGC   361 GAGGAGGATA ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA CATGGAGGGC   421 TCCGTGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC   481 ACCCAGACCG CCAAGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC CTGGGACATC   541 CTGTCCCCTC AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC CGACATCCCC   601 GACTACTTGA AGCTGTCCTT CCCCGAGGGC TTCAATTGGG AGCGCGTGAT GAACTTCGAG   661 GACGNNNNNN TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA GTTCATCTAC   721 AAGGTGAAGC TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA GTGTCGTACC   781 ATGGGCTGGG AGGCCTCCAC TGAGCGGATG TACCCCGAGG ANGGCGCCCT GAANGGNGAG   841 ATCAAGCAGA NGCTGAAGCT GAAGGACGGC GNCCACTACN ANGCTGANGN CAAGANCACC   901 TACAAGGNCN AGAAGCCCGT GCAGCTGCCC GGCGCNTNNN NCNNCNANNT CNAGTNGNAN   961 NTNCTTTNCC NCNNCNAGNN NNACACNNTC NTGNAACAGT ACNAANNNGN CNAGGNNCGC  1021 NNNTNNNNNN NNNGCNTNNN NNANCTGNNN NNGTANTCTN NANNNNGATC NTAATCNNNN 1081 NNNNNN //
Primer: reverse (Pr0099) Region: insert 3'(loxP.2,mPGK ter,BSD 3') Sequence file: RDB16821_A8Lpf.seq
>D06157B8_A8Lp_1_reverse_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNAAGGGNNN NAAAGCTGGG TACCGGATCC TAGTGAACCT    61 CTTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC GAAGTTATAT TAAGGGTTCC   121 GGATCTCGAC CAGCTTCTGA TGGAATTAGA ACTTGGCAAA ACAATACTGA GAATGAAGTG   181 TATGTGGAAC AGAGGCTGCT GATCTCGTTC TTCAGGCTAT GAAACTGACA CATTTGGAAA   241 CCACAGTACT TAGAACCACA AAGTGGGAAT CAAGAGAAAA ACAATGATCC CACGAGAGAT   301 CTATAGATCT ATAGATCATG AGTGGGAGGA ATGAGCTGGC CCTTAATTTG GTTTTGCTTG   361 TTTAAATTAT GATATCCAAC TATGAAACAT TATCATAAAG CAATAGTAAA GAGCCTTCAG   421 TAAAGAGCAG GCATTTATCT AATCCCACCC CACCCCCACC CCCGTAGCTC CAATCCTTCC   481 ATTCAAAATG TAGGTACTCT GTTCTCACCC TTCTTAACAA AGTATGACAG GAAAAACTTC   541 CATTTTAGTG GACATCTTTA TTGTTTAATA GATCATCAAT TTCTGCATCC CGGGGATCTG   601 ATATCATCGA TTAGCCCTCC CACACATAAC CAGAGGGCAG CAATTCACGA ATCCCAACTG   661 CCGTCGGCTG TCCATCACTG TCCTTCACTA TGGCTTTGAT CCCAGGATGC AGATCGAGAA   721 GCACCTGTCG GCACCGTCCG CAGGGGCTCA AGATGCCCCT GTTCTCATTT CCGATCGCGA   781 CGATACAAGT CAGGTTGCCN GCTGCCGCAG CAGCAGCAGT GCCCAGCACC ACGAGTTCTG   841 CACAANGTCC CCCAGTAAAA TGATATACAT TGACACCNGT GANNANGCGN NCGTCGCTNG   901 AGAGAGCTGN NNTGGNGANN CTGTANNCTT CNGAGANGGG GANGNTGNNG ATTGNAGNCG 961 NTGNTCTTNN NNNGNNGNNG GANTCTNCNN GANANNAAGG NTTNGNNNNN NNNNN //
Primer: PGK_N_rev (Pr0243) Region: insert mid(loxP.1,SV40 pA,mCherry2 3') Sequence file: RDB16821_A8Lpg.seq
>D06157B8_A8Lp_1_PGK_N_rev_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN CNGGNAGANN TTCGAGGTCG AGNNCCTAAG CTTGGCTGGA    61 CGTAAACTCC TCTTCAGACC TAATAACTTC GTATAGCATA CATTATACGA AGTTATATTA   121 AGGGTTATTG AATATGATCG ACGCGTTAAG ATACATTGAT GAGTTTGGAC AAACCACAAC   181 TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT   241 AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA   301 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT   361 GGCTGATTAT GATCAGTTAT CTAGATTACT TGTACAGCTC GTCCATGCCG CCGGTGGAGT   421 GGCGGCCCTC GGCGCGTTCG TACTGTTCCA CGATGGTGTA GTCCTCGTTG TGGGAAAGGA   481 TGTCCAACTT GATGTCGACG TTGTAGGCGC CGGGCAGCTG CACGGGCTTC TTGGCCTTGT   541 AGGTGGTCTT GACCTCAGCG TCGTAGTGGC CGCCGTCCTT CAGCTTCAGC CTCTGCTTGA   601 TCTCGCCCTT CAGGGCGCCG TCCTCGGGGT ACATCCGCTC AGTGGAGGCC TCCCAGCCCA   661 TGGTACGACA CTGCATTACG GGGCCGTCGG AGGGGAAGTT GGTGCCGCGC AGCTTCACCT   721 TGTAGATGAA CTCGCCGTCC TGCAGGGAGG AGTCCTGGGT CACGGTCACC ACGCCGCCGT   781 CCTCGAAGTT CATCACGCGC TCCCAATTGA AGCCCTCGGG GAAGGACAGC TTCAAGTAGT   841 CGGGGANGTC GNCGGGGTGC TTCACGTANG CCTTGNAGCC GTACATGANN TGANGGGANA   901 GGANGTCCCN NGCGAANGNN ANGGGGNCNC CCNNGGTCAC CNNCAGCTNG GNGGNCTGGG   961 TGNCNTNNTA NGGGNGGNCC TCGNNNNNGN CCNNNATNTN NAANTNGTGG NNGTNNNNNN  1021 ANNNNTNNNT GTGCACCNTN NNNNNNNTGN NNNNCNNNNN GANNNNNANN NNATNNNNNN 1081 NNNN //
Primer: PGKpro_F (Pr0249) Region: insert mid(BSD,mPGK ter) Sequence file: RDB16821_A8Lph.seq
>D06157B8_A8Lp_1_PGKpro_F_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTNN NNNNGCCTTT CGACCTGCAT CCNTCTAGAT    61 CTCGAGCCAC CATGGCCAAG CCTTTGTCTC AAGAAGAATC CACCCTCATT GAAAGAGCAA   121 CGGCTACAAT CAACAGCATC CCCATCTCTG AAGACTACAG CGTCGCCAGC GCAGCTCTCT   181 CTAGCGACGG CCGCATCTTC ACTGGTGTCA ATGTATATCA TTTTACTGGG GGACCTTGTG   241 CAGAACTCGT GGTGCTGGGC ACTGCTGCTG CTGCGGCAGC TGGCAACCTG ACTTGTATCG   301 TCGCGATCGG AAATGAGAAC AGGGGCATCT TGAGCCCCTG CGGACGGTGC CGACAGGTGC   361 TTCTCGATCT GCATCCTGGG ATCAAAGCCA TAGTGAAGGA CAGTGATGGA CAGCCGACGG   421 CAGTTGGGAT TCGTGAATTG CTGCCCTCTG GTTATGTGTG GGAGGGCTAA TCGATGATAT   481 CAGATCCCCG GGATGCAGAA ATTGATGATC TATTAAACAA TAAAGATGTC CACTAAAATG   541 GAAGTTTTTC CTGTCATACT TTGTTAAGAA GGGTGAGAAC AGAGTACCTA CATTTTGAAT   601 GGAAGGATTG GAGCTACGGG GGTGGGGGTG GGGTGGGATT AGATAANNGN NNGCTCTTNN   661 NNGAAGGCTC TTTACTATTG CNNNNNGATA ATGTTTCATA GTTGGATATC NNNNNTTAAN 721 NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.12.13

GNP_filter3_RDBDEP_html_231213.pl