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pMK288 (mAID-Bsr) (#RDB13931)

A plasmid for construction of C-terminal mAID tagging donors.

Alternative name mAID-Bsr, 1007
Clone info. A plasmid for construction of C-terminal mAID tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone.
Comment To make 0.5 M stock solution, dissolve indole-3-acetic acid (IAA) (Sigma-Aldrich, 45533) or 1-naphthaleneacetic acid (NAA) (Sigma-Aldrich, 35745) in 100 % DMSO. IAA solution can be made in 100 % ethanol. Aliquots can be kept for 6 months in the dark at -20oC (Devrekanli, A., Kanemaki, M.T. Methods in Molecular Biology, 1369, 257-278, 2016).
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA
Depositor|Developer Kanemaki, Masato |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。

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RDB13931 pMK288 (mAID-Bsr) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMK288 (mAID-Bsr) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13931).

Reference section:

Natsume, T., Kiyomitsu, T., Saga, Y., Kanemaki, M.T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13931_A6B8p1-4.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410)
Region: T7 pro, Linker, mAID, SV40 pA, loxP, PGK pro
Sequence file: RDB13931_A6B8g.seq check
>D03070A15_A6B8_1_M13(-40)_H03_24.ab1
    1 NNNNNNNNNC NCNNNNNNGN NTNNCNATAG GGCGAATTGG AGCTCCCCGG GATCCGGTGC
   61 AGGCGCCAAG GAGAAGAGTG CTTGTCCTAA AGATCCAGCC AAACCTCCGG CCAAGGCACA
  121 AGTTGTGGGA TGGCCACCGG TGAGATCATA CCGGAAGAAC GTGATGGTTT CCTGCCAAAA
  181 ATCAAGCGGT GGCCCGGAGG CGGCGGCGTT CGTGAAGGTA TCAATGGACG GAGCACCGTA
  241 CTTGAGGAAA ATCGATTTGA GGATGTATAA ATAATCTAGA TAACTGATCA TAATCAGCCA
  301 TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT
  361 GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA
  421 CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG
  481 TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAACGCGTC GATCATATTC AATAACCCTT
  541 AATATAACTT CGTATAATGT ATGCTATACG AAGTTATTAG GTCTGAAGAG GAGTTTACGT
  601 CCAGCCAAGC TTAGGATCTC GACCTCGAAA TTCTACCGGG TAGGGGAGGC GCTTTTCCCA
  661 AGGCAGTCTG GAGCATGCGC TTTAGCAGCC CCGCTGGGCA CTTGGCGCTA CACAAGTGGC
  721 CTCTGGCCTC GCACACATTC CACATCCACC GGTAGGCGCC AACCGACTCC GTTCTTTGGT
  781 GGCCCCTTCG CGCCACCTTC TACTCCTCCC CTAGTCANGA AGTTCCCCCC CGCCCCGCAN
  841 CTCGCGTCNT GCNNNNGTGA CAAATGGNAG TAGCACGTCT CACTAGTCTC GTGCANATNG
  901 ACAGCANCGC TNANCAATGG AAGCGGGNNN NNNNTTNGGG CNNCNNNCNA TANCANCNTN
961 GCTNNNNCNC TTTNNGGGCN NNNANNNNNG GNNNNGNNNN NNNNNN
//
Primer: M13R (Pr0160)
Region: loxP, mouse PGK ter, Bsr
Sequence file: RDB13931_A6B8h.seq check
>D03070A15_A6B8_1_M13R_D03_12.ab1
    1 NNNNNNNNNN NNNNNNNNNA AGGGNNNNAA AGCTGGGTAC CGGATCCTAG TGAACCTCTT
   61 CGAGGGACCT AATAACTTCG TATAGCATAC ATTATACGAA GTTATATTAA GGGTTCCGGA
  121 TCTCGACCAG CTTCTGATGG AATTAGAACT TGGCAAAACA ATACTGAGAA TGAAGTGTAT
  181 GTGGAACAGA GGCTGCTGAT CTCGTTCTTC AGGCTATGAA ACTGACACAT TTGGAAACCA
  241 CAGTACTTAG AACCACAAAG TGGGAATCAA GAGAAAAACA ATGATCCCAC GAGAGATCTA
  301 TAGATCTATA GATCATGAGT GGGAGGAATG AGCTGGCCCT TAATTTGGTT TTGCTTGTTT
  361 AAATTATGAT ATCCAACTAT GAAACATTAT CATAAAGCAA TAGTAAAGAG CCTTCAGTAA
  421 AGAGCAGGCA TTTATCTAAT CCCACCCCAC CCCCACCCCC GTAGCTCCAA TCCTTCCATT
  481 CAAAATGTAG GTACTCTGTT CTCACCCTTC TTAACAAAGT ATGACAGGAA AAACTTCCAT
  541 TTTAGTGGAC ATCTTTATTG TTTAATAGAT CATCAATTTC TGCATCCCGG GGATCTGATA
  601 TCATCGATGC ATGGGGTCGT GCGCTCCTTT CGGTCGGGCG CTGCGGGTCG TGGGGCGGGC
  661 GGCTAGCTTA ATTTCGGGTA TATTTGAGTG GAATGAGTTC TTCAATCGTA GTTTTGACTA
  721 ACTTGCCATT CATTTCTATT AACACAAAAC AATCTGGTGC ATAGTCTGAA ATCAACTCCC
  781 TACACATACC ACAAGGACTT ACCACTCGAA TACTTCTATC TACTTCGTCA GANTAANGGT
  841 GTCTAACAGC TACNATCGTG TCAAAATCCT TTTGTCNATT CGAAACTGCA CTACNNATCG
  901 NAATGGCTTC TGCNCAANCA GTTACTCGTC CNATATACGC TTCNNNNTGN ACNGNCNAAA
961 TGNNTTNNNC NGNTTTCNNA CGNNNTNNNC NNCTNCNNNN NNNNNN
//
Primer: PGKpro_F (Pr0249)
Region: Bsr, mouse PGK ter
Sequence file: RDB13931_A6B8i.seq check
>D03070A15_A6B8_1_PGK_proF_G10_19.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTCCGGGC CTTTCGACCT GCATCNNTCT AGATCTCGAG
   61 CAGCTGAAGC TTACCGCTAG CATGAAAACA TTTAACATTT CTCAACAAGA TCTAGAATTA
  121 GTAGAAGTAG CGACAGAGAA GATTACAATG CTTTATGAGG ATAATAAACA TCATGTGGGA
  181 GCGGCAATTC GTACGAAAAC AGGAGAAATC ATTTCGGCAG TACATATTGA AGCGTATATA
  241 GGACGAGTAA CTGTTTGTGC AGAAGCCATT GCGATTGGTA GTGCAGTTTC GAATGGACAA
  301 AAGGATTTTG ACACGATTGT AGCTGTTAGA CACCCTTATT CTGACGAAGT AGATAGAAGT
  361 ATTCGAGTGG TAAGTCCTTG TGGTATGTGT AGGGAGTTGA TTTCAGACTA TGCACCAGAT
  421 TGTTTTGTGT TAATAGAAAT GAATGGCAAG TTAGTCAAAA CTACGATTGA AGAACTCATT
  481 CCACTCAAAT ATACCCGAAA TTAAGCTAGC CGCCCGCCCC ACGACCCGCA GCGCCCGACC
  541 GAAAGGAGCG CACGACCCCA TGCATCGATG ATATCAGATC CCCGGGATGC AGAAATTGAT
  601 GATCTATTAA ACAATAAAGA TGTCCACTAA AATGGAAGTT TTTCCTGTCA TACTTTGTTA
  661 AGAAGGGTGA GAACAGAGTA CCTACATTTT GAATGGAAGG ATTGGAGCTA CGGGGGTGGG
  721 GGTGGGGTGG GATTAGATAA NTGNCTGCTC TTTACTGAAN GCTCTTTACT ATTGCTTTAT
  781 GANNAATGTT TCNTNGTTGG ATATCATNNT TTNNNCNANG CNNNCCNNNT TNNGGGNNNN
841 GNTCNNNNNN NNNCNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP]
reference Devrekanli, A., Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes. Methods in Molecular Biology 1369: 257-278 (2016). PMID 26519318.
reference Nishimura, K., Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Curr. Protoc. Cell Biol. \64: 20.9.1-16 (2014). PMID 25181302.