Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK288 (mAID-Bsr)

A plasmid for construction of C-terminal mAID tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

Ordering

Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13931_A6B8p1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Region: T7 pro, Linker, mAID, SV40 p(A), loxP, PGK pro Sequence file: RDB13931_A6B8d.seq
>D03070A15_A6B8_1_M13(-40)_H03_24.ab1     1 AAGCGGACGC GCGTATACGA CTCACTATAG GGCGAATTGG AGCTCCCCGG GATCCGGTGC    61 AGGCGCCAAG GAGAAGAGTG CTTGTCCTAA AGATCCAGCC AAACCTCCGG CCAAGGCACA   121 AGTTGTGGGA TGGCCACCGG TGAGATCATA CCGGAAGAAC GTGATGGTTT CCTGCCAAAA   181 ATCAAGCGGT GGCCCGGAGG CGGCGGCGTT CGTGAAGGTA TCAATGGACG GAGCACCGTA   241 CTTGAGGAAA ATCGATTTGA GGATGTATAA ATAATCTAGA TAACTGATCA TAATCAGCCA   301 TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT   361 GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA   421 CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG   481 TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAACGCGTC GATCATATTC AATAACCCTT   541 AATATAACTT CGTATAATGT ATGCTATACG AAGTTATTAG GTCTGAAGAG GAGTTTACGT   601 CCAGCCAAGC TTAGGATCTC GACCTCGAAA TTCTACCGGG TAGGGGAGGC GCTTTTCCCA   661 AGGCAGTCTG GAGCATGCGC TTTAGCAGCC CCGCTGGGCA CTTGGCGCTA CACAAGTGGC   721 CTCTGGCCTC GCACACATTC CACATCCACC GGTAGGCGCC AACCGACTCC GTTCTTTGGT   781 GGCCCCTTCG CGCCACCTTC TACTCCTCCC CTAGTCAGGA AGTTCCCCCC CGCCCCGCAG   841 CTCGCGTCGT GCAGACGTGA CAAATGGAAG TAGCACGTCT CACTAGTCTC GTGCAGATGG   901 ACAGCACCGC TGAGCAATGG AAGCGGGTAG GCCTTTGGGG CAGCGGCCAA TAGCAGCTTT   961 GCTCCTTCGC TTTCTGGGCT CAGAGGCTGG GGAAGGGTGG GTCCCGGGGG CGGCTCAGGG  1021 CGGGCTCAGG GCGGGCGGCG CCCGAGTCCT CGAGCCGGCA TTCTGCACGC TCAAAAGCGC  1081 ACGTCTGCCA GGCTGATCTC CTCTCTCAAT CTCGACCTTC GGACCTTGCC ATCCATCCTT 1141 GA //
Primer: PGKpro_F Region: PGK pro, Bsr, mouse PGK ter Sequence file: RDB13931_A6B8e.seq
>D03070A15_A6B8_1_PGK_proF_G10_19.ab1     1 AGGGGGCCGT TCTCTCTTCT CATCTCCGGG CCTTTCGACC TGCATCCATC TAGATCTCGA    61 GCAGCTGAAG CTTACCGCTA GCATGAAAAC ATTTAACATT TCTCAACAAG ATCTAGAATT   121 AGTAGAAGTA GCGACAGAGA AGATTACAAT GCTTTATGAG GATAATAAAC ATCATGTGGG   181 AGCGGCAATT CGTACGAAAA CAGGAGAAAT CATTTCGGCA GTACATATTG AAGCGTATAT   241 AGGACGAGTA ACTGTTTGTG CAGAAGCCAT TGCGATTGGT AGTGCAGTTT CGAATGGACA   301 AAAGGATTTT GACACGATTG TAGCTGTTAG ACACCCTTAT TCTGACGAAG TAGATAGAAG   361 TATTCGAGTG GTAAGTCCTT GTGGTATGTG TAGGGAGTTG ATTTCAGACT ATGCACCAGA   421 TTGTTTTGTG TTAATAGAAA TGAATGGCAA GTTAGTCAAA ACTACGATTG AAGAACTCAT   481 TCCACTCAAA TATACCCGAA ATTAAGCTAG CCGCCCGCCC CACGACCCGC AGCGCCCGAC   541 CGAAAGGAGC GCACGACCCC ATGCATCGAT GATATCAGAT CCCCGGGATG CAGAAATTGA   601 TGATCTATTA AACAATAAAG ATGTCCACTA AAATGGAAGT TTTTCCTGTC ATACTTTGTT   661 AAGAAGGGTG AGAACAGAGT ACCTACATTT TGAATGGAAG GATTGGAGCT ACGGGGGTGG 721 GGGTGGGGGT GGGAT //
Primer: M13R Region: T3 pro, loxP, mouse PGK ter, Bsr Sequence file: RDB13931_A6B8f.seq
>D03070A15_A6B8_1_M13R_D03_12.ab1     1 GTCGCGCATT ACCTCACTAA AGGGAACAAA AGCTGGGTAC CGGATCCTAG TGAACCTCTT    61 CGAGGGACCT AATAACTTCG TATAGCATAC ATTATACGAA GTTATATTAA GGGTTCCGGA   121 TCTCGACCAG CTTCTGATGG AATTAGAACT TGGCAAAACA ATACTGAGAA TGAAGTGTAT   181 GTGGAACAGA GGCTGCTGAT CTCGTTCTTC AGGCTATGAA ACTGACACAT TTGGAAACCA   241 CAGTACTTAG AACCACAAAG TGGGAATCAA GAGAAAAACA ATGATCCCAC GAGAGATCTA   301 TAGATCTATA GATCATGAGT GGGAGGAATG AGCTGGCCCT TAATTTGGTT TTGCTTGTTT   361 AAATTATGAT ATCCAACTAT GAAACATTAT CATAAAGCAA TAGTAAAGAG CCTTCAGTAA   421 AGAGCAGGCA TTTATCTAAT CCCACCCCAC CCCCACCCCC GTAGCTCCAA TCCTTCCATT   481 CAAAATGTAG GTACTCTGTT CTCACCCTTC TTAACAAAGT ATGACAGGAA AAACTTCCAT   541 TTTAGTGGAC ATCTTTATTG TTTAATAGAT CATCAATTTC TGCATCCCGG GGATCTGATA   601 TCATCGATGC ATGGGGTCGT GCGCTCCTTT CGGTCGGGCG CTGCGGGTCG TGGGGCGGGC   661 GGCTAGCTTA ATTTCGGGTA TATTTGAGTG GAATGAGTTC TTCAATCGTA GTTTTGACTA   721 ACTTGCCATT CATTTCTATT AACACAAAAC AATCTGGTGC ATAGTCTGAA ATCAACTCCC   781 TACACATACC ACAAGGACTT ACCACTCGAA TACTTCTATC TACTTCGTCA GAATAAGGGT   841 GTCTAACAGC TACAATCGTG TCAAAATCCT TTTGTCCATT CGAAACTGCA CTACCAATCG   901 CAATGGCTTC TGCACAAACA GTTACTCGTC CTATATACGC TTCAATATGT ACTGCCGAAA   961 TGATTTCTCC TGTTTTCGTA CGAATTTGCC CGCTCCCACA TGATGTTTAT TATCCTCATA  1021 AAGCATTGTA ATCTTCTCTG TCGCTACTCT ACTAAATTCT AGATCCTGGT GAGAAATGTC  1081 AATGTTTTCA TGCTAGCGTA GGCATCAGCT GCTTCGAGAT CTAGAATGAT GGCAGGATCC 1141 GA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl