Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK288 (mAID-Bsr)

A plasmid for construction of C-terminal mAID tagging donors.

Catalog number RDB13931
Resource name pMK288 (mAID-Bsr)
Alternative name mAID-Bsr, 1007
Clone info. A plasmid for construction of C-terminal mAID tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone.
Comment To make 0.5 M stock solution, dissolve indole-3-acetic acid (IAA) (Sigma-Aldrich, 45533) or 1-naphthaleneacetic acid (NAA) (Sigma-Aldrich, 35745) in 100 % DMSO. IAA solution can be made in 100 % ethanol. Aliquots can be kept for 6 months in the dark at -20oC (Devrekanli, A., Kanemaki, M.T. Methods in Molecular Biology, 1369, 257-278, 2016).
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank

External Database
Arabidopsis thaliana AtIAA17

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB13931 pMK288 (mAID-Bsr) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13931_A6B8p1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Region: T7 pro, Linker, mAID, SV40 p(A), loxP, PGK pro
          Sequence file: RDB13931_A6B8d.seq check
          >D03070A15_A6B8_1_M13(-40)_H03_24.ab1
              1 AAGCGGACGC GCGTATACGA CTCACTATAG GGCGAATTGG AGCTCCCCGG GATCCGGTGC
             61 AGGCGCCAAG GAGAAGAGTG CTTGTCCTAA AGATCCAGCC AAACCTCCGG CCAAGGCACA
            121 AGTTGTGGGA TGGCCACCGG TGAGATCATA CCGGAAGAAC GTGATGGTTT CCTGCCAAAA
            181 ATCAAGCGGT GGCCCGGAGG CGGCGGCGTT CGTGAAGGTA TCAATGGACG GAGCACCGTA
            241 CTTGAGGAAA ATCGATTTGA GGATGTATAA ATAATCTAGA TAACTGATCA TAATCAGCCA
            301 TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT
            361 GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA
            421 CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG
            481 TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAACGCGTC GATCATATTC AATAACCCTT
            541 AATATAACTT CGTATAATGT ATGCTATACG AAGTTATTAG GTCTGAAGAG GAGTTTACGT
            601 CCAGCCAAGC TTAGGATCTC GACCTCGAAA TTCTACCGGG TAGGGGAGGC GCTTTTCCCA
            661 AGGCAGTCTG GAGCATGCGC TTTAGCAGCC CCGCTGGGCA CTTGGCGCTA CACAAGTGGC
            721 CTCTGGCCTC GCACACATTC CACATCCACC GGTAGGCGCC AACCGACTCC GTTCTTTGGT
            781 GGCCCCTTCG CGCCACCTTC TACTCCTCCC CTAGTCAGGA AGTTCCCCCC CGCCCCGCAG
            841 CTCGCGTCGT GCAGACGTGA CAAATGGAAG TAGCACGTCT CACTAGTCTC GTGCAGATGG
            901 ACAGCACCGC TGAGCAATGG AAGCGGGTAG GCCTTTGGGG CAGCGGCCAA TAGCAGCTTT
            961 GCTCCTTCGC TTTCTGGGCT CAGAGGCTGG GGAAGGGTGG GTCCCGGGGG CGGCTCAGGG
           1021 CGGGCTCAGG GCGGGCGGCG CCCGAGTCCT CGAGCCGGCA TTCTGCACGC TCAAAAGCGC
           1081 ACGTCTGCCA GGCTGATCTC CTCTCTCAAT CTCGACCTTC GGACCTTGCC ATCCATCCTT
          1141 GA
          //
          Primer: PGKpro_F
          Region: PGK pro, Bsr, mouse PGK ter
          Sequence file: RDB13931_A6B8e.seq check
          >D03070A15_A6B8_1_PGK_proF_G10_19.ab1
              1 AGGGGGCCGT TCTCTCTTCT CATCTCCGGG CCTTTCGACC TGCATCCATC TAGATCTCGA
             61 GCAGCTGAAG CTTACCGCTA GCATGAAAAC ATTTAACATT TCTCAACAAG ATCTAGAATT
            121 AGTAGAAGTA GCGACAGAGA AGATTACAAT GCTTTATGAG GATAATAAAC ATCATGTGGG
            181 AGCGGCAATT CGTACGAAAA CAGGAGAAAT CATTTCGGCA GTACATATTG AAGCGTATAT
            241 AGGACGAGTA ACTGTTTGTG CAGAAGCCAT TGCGATTGGT AGTGCAGTTT CGAATGGACA
            301 AAAGGATTTT GACACGATTG TAGCTGTTAG ACACCCTTAT TCTGACGAAG TAGATAGAAG
            361 TATTCGAGTG GTAAGTCCTT GTGGTATGTG TAGGGAGTTG ATTTCAGACT ATGCACCAGA
            421 TTGTTTTGTG TTAATAGAAA TGAATGGCAA GTTAGTCAAA ACTACGATTG AAGAACTCAT
            481 TCCACTCAAA TATACCCGAA ATTAAGCTAG CCGCCCGCCC CACGACCCGC AGCGCCCGAC
            541 CGAAAGGAGC GCACGACCCC ATGCATCGAT GATATCAGAT CCCCGGGATG CAGAAATTGA
            601 TGATCTATTA AACAATAAAG ATGTCCACTA AAATGGAAGT TTTTCCTGTC ATACTTTGTT
            661 AAGAAGGGTG AGAACAGAGT ACCTACATTT TGAATGGAAG GATTGGAGCT ACGGGGGTGG
          721 GGGTGGGGGT GGGAT
          //
          Primer: M13R
          Region: T3 pro, loxP, mouse PGK ter, Bsr
          Sequence file: RDB13931_A6B8f.seq check
          >D03070A15_A6B8_1_M13R_D03_12.ab1
              1 GTCGCGCATT ACCTCACTAA AGGGAACAAA AGCTGGGTAC CGGATCCTAG TGAACCTCTT
             61 CGAGGGACCT AATAACTTCG TATAGCATAC ATTATACGAA GTTATATTAA GGGTTCCGGA
            121 TCTCGACCAG CTTCTGATGG AATTAGAACT TGGCAAAACA ATACTGAGAA TGAAGTGTAT
            181 GTGGAACAGA GGCTGCTGAT CTCGTTCTTC AGGCTATGAA ACTGACACAT TTGGAAACCA
            241 CAGTACTTAG AACCACAAAG TGGGAATCAA GAGAAAAACA ATGATCCCAC GAGAGATCTA
            301 TAGATCTATA GATCATGAGT GGGAGGAATG AGCTGGCCCT TAATTTGGTT TTGCTTGTTT
            361 AAATTATGAT ATCCAACTAT GAAACATTAT CATAAAGCAA TAGTAAAGAG CCTTCAGTAA
            421 AGAGCAGGCA TTTATCTAAT CCCACCCCAC CCCCACCCCC GTAGCTCCAA TCCTTCCATT
            481 CAAAATGTAG GTACTCTGTT CTCACCCTTC TTAACAAAGT ATGACAGGAA AAACTTCCAT
            541 TTTAGTGGAC ATCTTTATTG TTTAATAGAT CATCAATTTC TGCATCCCGG GGATCTGATA
            601 TCATCGATGC ATGGGGTCGT GCGCTCCTTT CGGTCGGGCG CTGCGGGTCG TGGGGCGGGC
            661 GGCTAGCTTA ATTTCGGGTA TATTTGAGTG GAATGAGTTC TTCAATCGTA GTTTTGACTA
            721 ACTTGCCATT CATTTCTATT AACACAAAAC AATCTGGTGC ATAGTCTGAA ATCAACTCCC
            781 TACACATACC ACAAGGACTT ACCACTCGAA TACTTCTATC TACTTCGTCA GAATAAGGGT
            841 GTCTAACAGC TACAATCGTG TCAAAATCCT TTTGTCCATT CGAAACTGCA CTACCAATCG
            901 CAATGGCTTC TGCACAAACA GTTACTCGTC CTATATACGC TTCAATATGT ACTGCCGAAA
            961 TGATTTCTCC TGTTTTCGTA CGAATTTGCC CGCTCCCACA TGATGTTTAT TATCCTCATA
           1021 AAGCATTGTA ATCTTCTCTG TCGCTACTCT ACTAAATTCT AGATCCTGGT GAGAAATGTC
           1081 AATGTTTTCA TGCTAGCGTA GGCATCAGCT GCTTCGAGAT CTAGAATGAT GGCAGGATCC
          1141 GA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.
          reference Devrekanli, A., Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes. Methods in Molecular Biology 1369: 257-278 (2016). PMID 26519318.
          reference Nishimura, K., Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Curr. Protoc. Cell Biol. \64: 20.9.1-16 (2014). PMID 25181302.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl