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pMK288 (mAID-Bsr)

A plasmid for construction of C-terminal mAID tagging donors.

Catalog number RDB13931
Resource name pMK288 (mAID-Bsr)
Alternative name mAID-Bsr, 1007
Clone info. A plasmid for construction of C-terminal mAID tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone.
To make 0.5 M stock solution, dissolve indole-3-acetic acid (IAA) (Sigma-Aldrich, 45533) or 1-naphthaleneacetic acid (NAA) (Sigma-Aldrich, 35745) in 100 % DMSO. IAA solution can be made in 100 % ethanol. Aliquots can be kept for 6 months in the dark at -20oC (Devrekanli, A., Kanemaki, M.T. Methods in Molecular Biology, 1369, 257-278, 2016).
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA
Depositor Kanemaki, Masato |
 
Other clones in our bank Arabidopsis thaliana AtIAA17 (KEGG orthology K14484) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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RDB13931 pMK288 (mAID-Bsr) DNA solution

cDNA Request [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)

References

original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13931_A6B8p1_v2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 RDB13931_A6B8a_2.seq
>D03070A15_A6B8_1_M13(-40)_H03_24.ab1
    1 AAGCGGACGC GCGTATACGA CTCACTATAG GGCGAATTGG AGCTCCCCGG GATCCGGTGC
   61 AGGCGCCAAG GAGAAGAGTG CTTGTCCTAA AGATCCAGCC AAACCTCCGG CCAAGGCACA
  121 AGTTGTGGGA TGGCCACCGG TGAGATCATA CCGGAAGAAC GTGATGGTTT CCTGCCAAAA
  181 ATCAAGCGGT GGCCCGGAGG CGGCGGCGTT CGTGAAGGTA TCAATGGACG GAGCACCGTA
  241 CTTGAGGAAA ATCGATTTGA GGATGTATAA ATAATCTAGA TAACTGATCA TAATCAGCCA
  301 TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT
  361 GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA
  421 CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG
  481 TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAACGCGTC GATCATATTC AATAACCCTT
  541 AATATAACTT CGTATAATGT ATGCTATACG AAGTTATTAG GTCTGAAGAG GAGTTTACGT
  601 CCAGCCAAGC TTAGGATCTC GACCTCGAAA TTCTACCGGG TAGGGGAGGC GCTTTTCCCA
  661 AGGCAGTCTG GAGCATGCGC TTTAGCAGCC CCGCTGGGCA CTTGGCGCTA CACAAGTGGC
  721 CTCTGGCCTC GCACACATTC CACATCCACC GGTAGGCGCC AACCGACTCC GTTCTTTGGT
  781 GGCCCCTTCG CGCCACCTTC TACTCCTCCC CTAGTCAGGA AGTTCCCCCC CGCCCCGCAG
  841 CTCGCGTCGT GCAGACGTGA CAAATGGAAG TAGCACGTCT CACTAGTCTC GTGCAGATGG
  901 ACAGCACCGC TGAGCAATGG AAGCGGGTAG GCCTTTGGGG CAGCGGCCAA TAGCAGCTTT
  961 GCTCCTTCGC TTTCTGGGCT CAGAGGCTGG GGAAGGGTGG GTCCCGGGGG CGGCTCAGGG
 1021 CGGGCTCAGG GCGGGCGGCG CCCGAGTCCT CGAGCCGGCA TTCTGCACGC TCAAAAGCGC
 1081 ACGTCTGCCA GGCTGATCTC CTCTCTCAAT CTCGACCTTC GGACCTTGCC ATCCATCCTT
1141 GA
//
Primer: PGKpro_F RDB13931_A6B8b_2.seq
>D03070A15_A6B8_1_PGK_proF_G10_19.ab1
    1 AGGGGGCCGT TCTCTCTTCT CATCTCCGGG CCTTTCGACC TGCATCCATC TAGATCTCGA
   61 GCAGCTGAAG CTTACCGCTA GCATGAAAAC ATTTAACATT TCTCAACAAG ATCTAGAATT
  121 AGTAGAAGTA GCGACAGAGA AGATTACAAT GCTTTATGAG GATAATAAAC ATCATGTGGG
  181 AGCGGCAATT CGTACGAAAA CAGGAGAAAT CATTTCGGCA GTACATATTG AAGCGTATAT
  241 AGGACGAGTA ACTGTTTGTG CAGAAGCCAT TGCGATTGGT AGTGCAGTTT CGAATGGACA
  301 AAAGGATTTT GACACGATTG TAGCTGTTAG ACACCCTTAT TCTGACGAAG TAGATAGAAG
  361 TATTCGAGTG GTAAGTCCTT GTGGTATGTG TAGGGAGTTG ATTTCAGACT ATGCACCAGA
  421 TTGTTTTGTG TTAATAGAAA TGAATGGCAA GTTAGTCAAA ACTACGATTG AAGAACTCAT
  481 TCCACTCAAA TATACCCGAA ATTAAGCTAG CCGCCCGCCC CACGACCCGC AGCGCCCGAC
  541 CGAAAGGAGC GCACGACCCC ATGCATCGAT GATATCAGAT CCCCGGGATG CAGAAATTGA
  601 TGATCTATTA AACAATAAAG ATGTCCACTA AAATGGAAGT TTTTCCTGTC ATACTTTGTT
  661 AAGAAGGGTG AGAACAGAGT ACCTACATTT TGAATGGAAG GATTGGAGCT ACGGGGGTGG
721 GGGTGGGGGT GGGAT
//
Primer: M13R RDB13931_A6B8c_2.seq
>D03070A15_A6B8_1_M13R_D03_12.ab1
    1 GTCGCGCATT ACCTCACTAA AGGGAACAAA AGCTGGGTAC CGGATCCTAG TGAACCTCTT
   61 CGAGGGACCT AATAACTTCG TATAGCATAC ATTATACGAA GTTATATTAA GGGTTCCGGA
  121 TCTCGACCAG CTTCTGATGG AATTAGAACT TGGCAAAACA ATACTGAGAA TGAAGTGTAT
  181 GTGGAACAGA GGCTGCTGAT CTCGTTCTTC AGGCTATGAA ACTGACACAT TTGGAAACCA
  241 CAGTACTTAG AACCACAAAG TGGGAATCAA GAGAAAAACA ATGATCCCAC GAGAGATCTA
  301 TAGATCTATA GATCATGAGT GGGAGGAATG AGCTGGCCCT TAATTTGGTT TTGCTTGTTT
  361 AAATTATGAT ATCCAACTAT GAAACATTAT CATAAAGCAA TAGTAAAGAG CCTTCAGTAA
  421 AGAGCAGGCA TTTATCTAAT CCCACCCCAC CCCCACCCCC GTAGCTCCAA TCCTTCCATT
  481 CAAAATGTAG GTACTCTGTT CTCACCCTTC TTAACAAAGT ATGACAGGAA AAACTTCCAT
  541 TTTAGTGGAC ATCTTTATTG TTTAATAGAT CATCAATTTC TGCATCCCGG GGATCTGATA
  601 TCATCGATGC ATGGGGTCGT GCGCTCCTTT CGGTCGGGCG CTGCGGGTCG TGGGGCGGGC
  661 GGCTAGCTTA ATTTCGGGTA TATTTGAGTG GAATGAGTTC TTCAATCGTA GTTTTGACTA
  721 ACTTGCCATT CATTTCTATT AACACAAAAC AATCTGGTGC ATAGTCTGAA ATCAACTCCC
  781 TACACATACC ACAAGGACTT ACCACTCGAA TACTTCTATC TACTTCGTCA GAATAAGGGT
  841 GTCTAACAGC TACAATCGTG TCAAAATCCT TTTGTCCATT CGAAACTGCA CTACCAATCG
  901 CAATGGCTTC TGCACAAACA GTTACTCGTC CTATATACGC TTCAATATGT ACTGCCGAAA
  961 TGATTTCTCC TGTTTTCGTA CGAATTTGCC CGCTCCCACA TGATGTTTAT TATCCTCATA
 1021 AAGCATTGTA ATCTTCTCTG TCGCTACTCT ACTAAATTCT AGATCCTGGT GAGAAATGTC
 1081 AATGTTTTCA TGCTAGCGTA GGCATCAGCT GCTTCGAGAT CTAGAATGAT GGCAGGATCC
1141 GA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.
reference Devrekanli, A., Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes. Methods in Molecular Biology 1369: 257-278 (2016). PMID 26519318.
reference Nishimura, K., Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Curr. Protoc. Cell Biol. \64: 20.9.1-16 (2014). PMID 25181302 .

2018.12.11

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