Sequencing and PCR primers for primer information.
A plasmid for construction of C-terminal mAID tagging donors.
| Catalog number | RDB13931 |
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| Resource name | pMK288 (mAID-Bsr) |
| Alternative name | mAID-Bsr, 1007 |
| Clone info. | A plasmid for construction of C-terminal mAID tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone. To make 0.5 M stock solution, dissolve indole-3-acetic acid (IAA) (Sigma-Aldrich, 45533) or 1-naphthaleneacetic acid (NAA) (Sigma-Aldrich, 35745) in 100 % DMSO. IAA solution can be made in 100 % ethanol. Aliquots can be kept for 6 months in the dark at -20oC (Devrekanli, A., Kanemaki, M.T. Methods in Molecular Biology, 1369, 257-278, 2016). |
| Vector backbone | pBluescriptII KS- (Plasmid) |
| Size of vector backbone | 3.0 kb |
| Selectable markers | Am^r |
| Gene/insert name | Arabidopsis thaliana AtIAA17 cDNA |
| Depositor | Kanemaki, Masato | |
| Other clones in our bank | Arabidopsis thaliana AtIAA17 (KEGG orthology K14484) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB13931 | pMK288 (mAID-Bsr) | DNA solution |
Electronic file
Featured content
| Featured content | Auxin Inducible Degron (AID) System (English text) |
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| Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
References
| original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB13931_A6B8p1_v2.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: M13_-40 | RDB13931_A6B8a_2.seq |
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>D03070A15_A6B8_1_M13(-40)_H03_24.ab1 1 AAGCGGACGC GCGTATACGA CTCACTATAG GGCGAATTGG AGCTCCCCGG GATCCGGTGC 61 AGGCGCCAAG GAGAAGAGTG CTTGTCCTAA AGATCCAGCC AAACCTCCGG CCAAGGCACA 121 AGTTGTGGGA TGGCCACCGG TGAGATCATA CCGGAAGAAC GTGATGGTTT CCTGCCAAAA 181 ATCAAGCGGT GGCCCGGAGG CGGCGGCGTT CGTGAAGGTA TCAATGGACG GAGCACCGTA 241 CTTGAGGAAA ATCGATTTGA GGATGTATAA ATAATCTAGA TAACTGATCA TAATCAGCCA 301 TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT 361 GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA 421 CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG 481 TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAACGCGTC GATCATATTC AATAACCCTT 541 AATATAACTT CGTATAATGT ATGCTATACG AAGTTATTAG GTCTGAAGAG GAGTTTACGT 601 CCAGCCAAGC TTAGGATCTC GACCTCGAAA TTCTACCGGG TAGGGGAGGC GCTTTTCCCA 661 AGGCAGTCTG GAGCATGCGC TTTAGCAGCC CCGCTGGGCA CTTGGCGCTA CACAAGTGGC 721 CTCTGGCCTC GCACACATTC CACATCCACC GGTAGGCGCC AACCGACTCC GTTCTTTGGT 781 GGCCCCTTCG CGCCACCTTC TACTCCTCCC CTAGTCAGGA AGTTCCCCCC CGCCCCGCAG 841 CTCGCGTCGT GCAGACGTGA CAAATGGAAG TAGCACGTCT CACTAGTCTC GTGCAGATGG 901 ACAGCACCGC TGAGCAATGG AAGCGGGTAG GCCTTTGGGG CAGCGGCCAA TAGCAGCTTT 961 GCTCCTTCGC TTTCTGGGCT CAGAGGCTGG GGAAGGGTGG GTCCCGGGGG CGGCTCAGGG 1021 CGGGCTCAGG GCGGGCGGCG CCCGAGTCCT CGAGCCGGCA TTCTGCACGC TCAAAAGCGC 1081 ACGTCTGCCA GGCTGATCTC CTCTCTCAAT CTCGACCTTC GGACCTTGCC ATCCATCCTT 1141 GA // |
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| Primer: PGKpro_F | RDB13931_A6B8b_2.seq |
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>D03070A15_A6B8_1_PGK_proF_G10_19.ab1 1 AGGGGGCCGT TCTCTCTTCT CATCTCCGGG CCTTTCGACC TGCATCCATC TAGATCTCGA 61 GCAGCTGAAG CTTACCGCTA GCATGAAAAC ATTTAACATT TCTCAACAAG ATCTAGAATT 121 AGTAGAAGTA GCGACAGAGA AGATTACAAT GCTTTATGAG GATAATAAAC ATCATGTGGG 181 AGCGGCAATT CGTACGAAAA CAGGAGAAAT CATTTCGGCA GTACATATTG AAGCGTATAT 241 AGGACGAGTA ACTGTTTGTG CAGAAGCCAT TGCGATTGGT AGTGCAGTTT CGAATGGACA 301 AAAGGATTTT GACACGATTG TAGCTGTTAG ACACCCTTAT TCTGACGAAG TAGATAGAAG 361 TATTCGAGTG GTAAGTCCTT GTGGTATGTG TAGGGAGTTG ATTTCAGACT ATGCACCAGA 421 TTGTTTTGTG TTAATAGAAA TGAATGGCAA GTTAGTCAAA ACTACGATTG AAGAACTCAT 481 TCCACTCAAA TATACCCGAA ATTAAGCTAG CCGCCCGCCC CACGACCCGC AGCGCCCGAC 541 CGAAAGGAGC GCACGACCCC ATGCATCGAT GATATCAGAT CCCCGGGATG CAGAAATTGA 601 TGATCTATTA AACAATAAAG ATGTCCACTA AAATGGAAGT TTTTCCTGTC ATACTTTGTT 661 AAGAAGGGTG AGAACAGAGT ACCTACATTT TGAATGGAAG GATTGGAGCT ACGGGGGTGG 721 GGGTGGGGGT GGGAT // |
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| Primer: M13R | RDB13931_A6B8c_2.seq |
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>D03070A15_A6B8_1_M13R_D03_12.ab1 1 GTCGCGCATT ACCTCACTAA AGGGAACAAA AGCTGGGTAC CGGATCCTAG TGAACCTCTT 61 CGAGGGACCT AATAACTTCG TATAGCATAC ATTATACGAA GTTATATTAA GGGTTCCGGA 121 TCTCGACCAG CTTCTGATGG AATTAGAACT TGGCAAAACA ATACTGAGAA TGAAGTGTAT 181 GTGGAACAGA GGCTGCTGAT CTCGTTCTTC AGGCTATGAA ACTGACACAT TTGGAAACCA 241 CAGTACTTAG AACCACAAAG TGGGAATCAA GAGAAAAACA ATGATCCCAC GAGAGATCTA 301 TAGATCTATA GATCATGAGT GGGAGGAATG AGCTGGCCCT TAATTTGGTT TTGCTTGTTT 361 AAATTATGAT ATCCAACTAT GAAACATTAT CATAAAGCAA TAGTAAAGAG CCTTCAGTAA 421 AGAGCAGGCA TTTATCTAAT CCCACCCCAC CCCCACCCCC GTAGCTCCAA TCCTTCCATT 481 CAAAATGTAG GTACTCTGTT CTCACCCTTC TTAACAAAGT ATGACAGGAA AAACTTCCAT 541 TTTAGTGGAC ATCTTTATTG TTTAATAGAT CATCAATTTC TGCATCCCGG GGATCTGATA 601 TCATCGATGC ATGGGGTCGT GCGCTCCTTT CGGTCGGGCG CTGCGGGTCG TGGGGCGGGC 661 GGCTAGCTTA ATTTCGGGTA TATTTGAGTG GAATGAGTTC TTCAATCGTA GTTTTGACTA 721 ACTTGCCATT CATTTCTATT AACACAAAAC AATCTGGTGC ATAGTCTGAA ATCAACTCCC 781 TACACATACC ACAAGGACTT ACCACTCGAA TACTTCTATC TACTTCGTCA GAATAAGGGT 841 GTCTAACAGC TACAATCGTG TCAAAATCCT TTTGTCCATT CGAAACTGCA CTACCAATCG 901 CAATGGCTTC TGCACAAACA GTTACTCGTC CTATATACGC TTCAATATGT ACTGCCGAAA 961 TGATTTCTCC TGTTTTCGTA CGAATTTGCC CGCTCCCACA TGATGTTTAT TATCCTCATA 1021 AAGCATTGTA ATCTTCTCTG TCGCTACTCT ACTAAATTCT AGATCCTGGT GAGAAATGTC 1081 AATGTTTTCA TGCTAGCGTA GGCATCAGCT GCTTCGAGAT CTAGAATGAT GGCAGGATCC 1141 GA // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. |
|---|---|
| reference | Devrekanli, A., Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes. Methods in Molecular Biology 1369: 257-278 (2016). PMID 26519318. |
| reference | Nishimura, K., Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Curr. Protoc. Cell Biol. \64: 20.9.1-16 (2014). PMID 25181302 . |
2018.12.11
GNP_filter3_RDBDEP_html_181012.pl