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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK198 (#RDB18341)

A plasmid for generate yeast lines expressing OsTIR1(wild type) under the GAL promoter.

Alternative name GAL-OsTIR1
Clone info. A plasmid for generate yeast lines expressing OsTIR1(wild type) under the GAL promoter, see Fig. 2C and SFig. 4D of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone pBluescriptII KS- (plasmid)
Selectable markers Ampicillin (E. coli), URA3 (S. cerevisiae).
Gene/insert name Oryza sativa OsTIR1 cDNA
Depositor|Developer Kanemaki, Masato |
 
Sequence (full) RDB18341zzk02.seq provided by the depositor

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18341 pMK198 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMK198 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18341).

Reference section:

Yesbolatova, A., Saito, Y., Kitamoto, N., Makino-Itou, H., Ajima, R., Nakano, R., Nakaoka, H., Fukui, K., Gamo, K., Tominari, Y., Takeuchi, H., Saga, Y., Hayashi, K., Kanemaki, M.T., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18341_B0Gfp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-CAPbs_up_F2 (Pr0739)
Region: insert 5'(GAL1-10 pro)
Sequence file: RDB18341_B0Gfa.seq check
>D07105A9_B0Gf_1_pUC-CAPbs_up_F2_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GGCTTNNNNC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA
   61 ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCTC
  121 GAAATTAACC CTCACTAAAG GGAACAAAAG CTGGTACCGG GCCCCCCCTC GAGGTCGACG
  181 GTATCGATAA GCTTGATATC GAATTCGACA GGTTATCAGC AACAACACAG TCATATCCAT
  241 TCTCAATTAG CTCTACCACA GTGTGTGAAC CAATGTATCC AGCACCACCT GTAACCAAAA
  301 CAATTTTAGA AGTACTTTCA CTTTGTAACT GAGCTGTCAT TTATATTGAA TTTTCAAAAA
  361 TTCTTACTTT TTTTTTGGAT GGACGCAAAG AAGTTTAATA ATCATATTAC ATGGCATTAC
  421 CACCATATAC ATATCCATAT ACATATCCAT ATCTAATCTT ACTTATATGT TGTGGAAATG
  481 TAAAGAGCCC CATTATCTTA GCCTAAAAAA ACCTTCTCTT TGGAACTTTC AGTAATACGC
  541 TTAACTGCTC ATTGCTATAT TGAAGTACGG ATTAGAAGCC GCCGAGCGGG TGACAGCCCT
  601 CCGAAGGAAG ACTCTCCTCC GTGCGTCCTC GTCTTCACCG GTCGCGTTCC TGAAACGCAG
  661 ATGTGCCTCG CGCCGCACTG CTCCGAACAA TAAAGATTCT ACAATACTAG CTTTTATGGT
  721 TATGAAGAGG AAAAATTGGC AGTAACCTGG CCCCACAAAC CTTCNAATGA ACGAATCAAA
  781 TTAACAACCA TAGGATGATA ATGCGATTAG TTTTTTAGCC TTATTTCTGG GGTAATTAAT
  841 CAGCGAAGCG ATGANTTTTG ATCTATTANC AGATATATAA ATGCNAAANC TGCATAACCA
  901 CTTTANCTAA TACTTTCAAC ATTTTCGGNT TGNATTACTT CNNATTCNAN TGNAATAAAA
  961 NTATCNANAA AAAATTGNTN ATATACCTCT ANNCNTTNNN GTCNANGNAN AAAAAACCCN
1021 NGNNNCNNCN ANTATGANNN NNTTTNCNNG NNNNNNNNNN
//
Primer: GAL1pro_F2 (Pr0975)
Region: insert mid(GAL1-10 pro,OsTIR1 5')
Sequence file: RDB18341_B0Gfb.seq check
>D07105A9_B0Gf_1_GAL1pro_F2_A06_01_ABI08.ab1
    1 NNNNNNNNNT TNNNNNTNGN NNTACTTCTT ANNNNNNNGT AATAAAAGTA TCAACAAAAA
   61 ATTGTTAATA TACCTCTATA CTTTAACGTC AAGGAGAAAA AACCCCGGAT CCACTAGTAT
  121 GACTTATTTT CCTGAAGAAG TTGTAGAACA CATATTTTCC TTTTTACCTG CTCAAAGAGA
  181 TAGAAACACC GTCTCCTTGG TCTGTAAGGT TTGGTACGAA ATCGAAAGAT TGTCTAGAAG
  241 AGGTGTTTTT GTTGGTAATT GTTACGCAGT TAGAGCTGGT AGAGTTGCTG CAAGATTCCC
  301 AAACGTTAGA GCATTGACTG TTAAAGGTAA ACCTCATTTT GCTGATTTCA ATTTGGTTCC
  361 ACCTGATTGG GGTGGTTATG CTGGTCCATG GATTGAAGCT GCAGCTAGAG GTTGTCACGG
  421 TTTGGAAGAA TTGAGAATGA AGAGAATGGT TGTTTCTGAT GAATCATTGG AATTGTTAGC
  481 AAGATCTTTT CCTAGATTCA GAGCTTTGGT TTTGATCTCA TGTGAAGGTT TTTCCACCGA
  541 TGGTTTAGCA GCTGTTGCTT CACATTGTAA GTTGTTGAGA GAATTGGATT TGCAAGAAAA
  601 CGAAGTTGAA GATAGAGGTC CAAGATGGTT ATCTTGTTTT CCTGATTCCT GTACAAGTTT
  661 GGTTTCTTTG AACTTCGCAT GTATCAAGGG TGAAGTTAAC GCTGGTAGTT TGGAAAGATT
  721 AGTTTCTAGA TCACCAAATT TGAGATCTTT GAGANTGAAC AGATCCGTTA GTGTTGATAC
  781 CTTGGCAAAG ATCTTGTTGA GAACTCCTAA TTTGGAAGAN TTGGGTACTG GTAACTTGNC
  841 AGANGANTTC NNNACAGAAT CATACTTCAN GTTNANCNNC GCTTTGNAAA AGTGNAAGAN
  901 GNTGANATCT TTGTCAGGNT TTTGGGNNGC ANNNCCNGTT NGNTTGNNNT TTANNNATCC
961 TTTGNNNGCT CNNNTNGANN GNNTNGNANT TNNNNNTNNN NNNNNNN
//
Primer: pBAC_lac_F (Pr0544)
Region: insert mid(OsTIR1 3')
Sequence file: RDB18341_B0Gfc.seq check
>D07105A9_B0Gf_1_pBAC_lac_F_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GNAANANGAC TNNCTATAGG GCGAATTGGA GCTCCNCCGC
   61 GGTGGCGGCC GCTCATAAAA TCTTGACGAA GTTAGGAGCA TCATCTCTGG CACCTGCGGT
  121 TGTTCTGTAG ACGTATAACT TTTCAACTTT AGGCAAGTCA CCATGGTTTT CTTCCATTTC
  181 ATTGGAACCA TCTCTTTCGT TAATAACTTC AACGTTCAAC ATTGGCATCT TTGAAGCTAA
  241 AACTTGACAA CCTTTCAAGG TAACGTTACA ACTGGACATC CACAAAGATC TCATTGTTTC
  301 ATATCTTGCG AAATTACCCA ACAAAGCTGC GTCACCAAAT GGAGAATCTC TGATTTCCAA
  361 CTTTCTTAAG TTCTTACAAC CGTTCATAAC ATGCATCATA CCCTTATCGG AGTCACCAGC
  421 GAATGCGATT GACAACATTT CCAATTGCTT AGCGTATTTA CCGATGTACA TAAAAACCTT
  481 ATCTGTCAAC AAACCACTGA TGGACAATCT TTGCAAACCC TTACACTCTC TAACGATTGC
  541 ACCAAAACCT TCATCTAAAG GTTGTGATGT AACAACATCA GGTTTACCTG GTTCCAAGAT
  601 ACACAATCTG AATCTAGTGA AGTTTGGACA ATTTTTAGCA ACAGTAACCA AAGCTGCGTT
  661 GGTCATTTGA TGACAGAAGT ACAACAAGGA GTTCAACTTA GGACAACCCA ATGAAACTGC
  721 AACTAAACCT TCTTCTGTAA CAGCAGAGTA ACCTGCAACA TAGAAATCAC TTGGAAAAAC
  781 TCTCAATTCT TGCAAATCCT TACAAGAAGA AGCAACAACT TGCAAACCTT TATCTGAGAT
  841 ACAATCNAAA ACCCACAATC TTTGCAACTT AACACATCTA GAGATCATCT TTGTCAAATC
  901 ACTAGCATCN NAAGTTGGNG CGNAAGANAA ATTCAAACCA GTCNATTGAG CACACAAAGG
  961 ANATATAAAA NANAAANNAA CTGGNNTTGN NTCCNNAAAA NNNGNNNAAN ATNNCNNNAT
1021 NNNANNNTTT TNNNAANNNN ANNNNNNCNT NNNNNNNNNN
//
Primer: URA3pro_F (Pr0170)
Region: insert 3'(URA3 pro, URA3)
Sequence file: RDB18341_B0Gfd.seq check
>D07105A9_B0Gf_1_URA3pro_F_D03_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNGNANNTG CCNNGTATTC TTAACCCAAC TGCACAGAAC
   61 AAAAACCTGC AGGAAACGAA GATAAATCAT GTCGAAAGCT ACATATAAGG AACGTGCTGC
  121 TACTCATCCT AGTCCTGTTG CTGCCAAGCT ATTTAATATC ATGCACGAAA AGCAAACAAA
  181 CTTGTGTGCT TCATTGGATG TTCGTACCAC CAAGGAATTA CTGGAGTTAG TTGAAGCATT
  241 AGGTCCCAAA ATTTGTTTAC TAAAAACACA TGTGGATATC TTGACTGATT TTTCCATGGA
  301 GGGCACAGTT AAGCCGCTAA AGGCATTATC CGCCAAGTAC AATTTTTTAC TCTTCGAAGA
  361 CAGAAAATTT GCTGACATTG GTAATACAGT CAAATTGCAG TACTCTGCGG GTGTATACAG
  421 AATAGCAGAA TGGGCAGACA TTACGAATGC ACACGGTGTG GTGGGCCCAG GTATTGTTAG
  481 CGGTTTGAAG CAGGCGGCAG AAGAAGTAAC AAAGGAACCT AGAGGCCTTT TGATGTTAGC
  541 AGAATTGTCA TGCAAGGGCT CCCTATCTAC TGGAGAATAT ACTAAGGGTA CTGTTGACAT
  601 TGCGAAGAGC GACAAAGATT TTGTTATCGG CTTTATTGCT CAAAGAGACA TGGGTGGAAG
  661 AGATGAAGGT TACGATTGGT TGATTATGAC ACCCGGTGTG GGTTTAGATG ACAAGGGAGA
  721 CGCATTGGGT CAACAGTATA GAACCGTGGA TGATGTGGTC TCTACAGGAT CTGACATTAT
  781 TATTGTTGGA AGAGGACTAT TTGCAAAGGG AAGGGATGCT AAGGTAGAGG GTGAACGTTA
  841 CAGAAAAGCA NGCTGGGAAN CATATTTGAG AAGATGCGGC CAGCAAAACT AAAAAACTGT
  901 ATTATAAGTA AATGNNTGTA TACTAAACTC NNNAATTAGA GCTNNNNNTT AATTATATCA
  961 GTTATTANCC TATGCGGNGT GAAATANCGN NCAGANGCGN AANGAGAAAA NANCNNATCN
 1021 NNAANNGNNA GCNNTNNNNT TTTGNNAAAN TCNNNNNNAN NTTTNNNNNA NNNNNNNNNT
1081 TTTTTNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]