Sequencing and PCR primers for primer information.
A plasmid for construction of N-terminal BSD-P2A-mAID-mClover tagging donors.
| Catalog number | RDB16809 |
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| Resource name | pMK348 (BSD-P2A-mAID-mClover) |
| Clone info. | A plasmid for construction of N-terminal BSD-P2A-mAID-mClover tagging donors. |
| Vector backbone | pBluescript (plasmid) |
| Size of vector backbone | 2.9 kb |
| Selectable markers | Am^r (E. coli) |
| Gene/insert name | Arabidopsis thaliana AtIAA17 cDNA Aequorea victoria GFP cDNA |
| Depositor | Kanemaki, Masato | |
| Other clones in our bank | Arabidopsis thaliana AXR3 (KEGG orthology K14484) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB16809 | pMK348 (BSD-P2A-mAID-mClover) | DNA solution |
Electronic file
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Auxin Inducible Degron (AID) System (English text) |
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| Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB16809_A8Lpp1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pUC-M13_-100 | RDB16809_A8Lpa.seq |
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>D06157A5_A8LP_2_pUC-M13_-100_A04_01_ABI24.ab1 1 GGCGGTCAAG CGGATTAGTT GGGTACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG 61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGGTAC CGGGCCCCCC 121 CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGCCACCA TGGCCAAGCC TTTGTCTCAA 181 GAAGAATCCA CCCTCATTGA AAGAGCAACG GCTACAATCA ACAGCATCCC CATCTCTGAA 241 GACTACAGCG TCGCCAGCGC AGCTCTCTCT AGCGACGGCC GCATCTTCAC TGGTGTCAAT 301 GTATATCATT TTACTGGGGG ACCTTGTGCA GAACTCGTGG TGCTGGGCAC TGCTGCTGCT 361 GCGGCAGCTG GCAACCTGAC TTGTATCGTC GCGATCGGAA ATGAGAACAG GGGCATCTTG 421 AGCCCCTGCG GACGGTGCCG ACAGGTGCTT CTCGATCTGC ATCCTGGGAT CAAAGCCATA 481 GTGAAGGACA GTGATGGACA GCCGACGGCA GTTGGGATTC GTGAATTGCT GCCCTCTGGT 541 TATGTGTGGG AGGGCTCAGG AAGCGGAGCT ACTAACTTCA GCCTGCTGAA GCAGGCTGGA 601 GACGTGGAGG AGAACCCTGG ACCTAGATCT AAGGAGAAGA GTGCTTGTCC TAAAGATCCA 661 GCCAAACCTC CGGCCAAGGC ACAAGTTGTG GGATGGCCAC CGGTGAGATC ATACCGGAAG 721 AACGTGATGG TTTCCTGCCA AAAATCAAGC GGTGGCCCGG AGGCGGCGGC GTTCGTGAAG 781 GTATCAATGG ACGGAGCACC GTACTTGAGG AAAATCGATT TGAGGATGTA TAAAGCTAGC 841 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC 901 GGCGACGTAA ACGGGCCACA AGTTCAGCGT CCGCGGCGAG GGCGAGGGCG ATGCCACCCA 961 ACGGGCAAGC TGACCCTGAA GTTTCATCTG CACACCGGGC AAGCTGCCCG TGCCCTGGCC 1021 ACCTCGTGGA CCACCTTCGG CTACCGCCGT TACCTGCCTT CAGTCGCTAC TCGGAACTAC 1081 ATGGAAGGCC AGCAACGCAC CATACGATTA // |
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| Primer: reverse | RDB16809_A8Lpb.seq |
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>D06157A5_A8LP_2_reverse_B04_04_ABI24.ab1 1 CAAAAAAATA ACCCCTCACT AAAGGGACAA AAGCTGGAGC TCCACCGCGG TGGCGGCCGC 61 TCTAGAACTA GTGGATCCGG CGCCTGCACC AGCGCCCTTG TACAGCTCGT CCATGCCATG 121 TGTAATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG 181 GTCTTTGCTC AGCTTGGACT GATGGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC 241 GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCTCAAC 301 GTTGTGGCGG ATCTTGAAGT TAGCCTTGAT GCCGTTCTTC TGCTTGTCGG CCGTGATATA 361 GACGTTGTGG CTGTTGAAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT 421 GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC 481 GGCGCGGGTC TTGTAGGTAC CGTCGTCCTT GAAAGAGATG GTGCGCTCCT GGACGTAGCC 541 TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGCTGAA 601 GCAGGCCACG CCGTAGCCGA AGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC 661 GGTGGTGCAG ATGAACTTCA GGGTCAGCTT GCCGTTGGTG GCATCGCCCT CGCCCTCGCC 721 GCGGACGCTG AACTTGTGGC CGTTTACGTC GCCGTCCAGC TCGACCAGGA TGGGCACCAC 781 CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC CATGCTAGCT TTATACATCC TCAAATCGAT 841 TTTCCTCAAG TACGGTGCTC CGTCCATTGA TACCTTCACG AACGCCGCCG CCTCCGGGCC 901 ACCGCTTGAT TTTTGGCAGG AAACCATCAC GTTCTTCCGG TATGATCTCA CCGGGTGGCC 961 ATCCCACAAC TTTGTGCCTT TGACCGGAGG TTTGGCTGGA TCTTTAGACA GCACTTCTCC 1021 TCATTAGATC TAGGTCCAGG GATCTCTTCA CGTCTCAGCC TGCTCAGCAG GCTGCAGTTA 1081 GTAGCATCTG CATCTGAGTC TACCTCATGA TA // |
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Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
2019.07.12
GNP_filter3_RDBDEP_html_190431.pl