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pMK348 (BSD-P2A-mAID-mClover)

A plasmid for construction of N-terminal BSD-P2A-mAID-mClover tagging donors.

Catalog number RDB16809
Resource name pMK348 (BSD-P2A-mAID-mClover)
Clone info. A plasmid for construction of N-terminal BSD-P2A-mAID-mClover tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Aequorea victoria GFP cDNA
Depositor Kanemaki, Masato |
 
Other clones in our bank Arabidopsis thaliana AXR3 (KEGG orthology K14484) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering Please visit Information of Request for Distribution. 
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16809 pMK348 (BSD-P2A-mAID-mClover) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16809_A8Lpp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 RDB16809_A8Lpa.seq
>D06157A5_A8LP_2_pUC-M13_-100_A04_01_ABI24.ab1
    1 GGCGGTCAAG CGGATTAGTT GGGTACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG
   61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGGTAC CGGGCCCCCC
  121 CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGCCACCA TGGCCAAGCC TTTGTCTCAA
  181 GAAGAATCCA CCCTCATTGA AAGAGCAACG GCTACAATCA ACAGCATCCC CATCTCTGAA
  241 GACTACAGCG TCGCCAGCGC AGCTCTCTCT AGCGACGGCC GCATCTTCAC TGGTGTCAAT
  301 GTATATCATT TTACTGGGGG ACCTTGTGCA GAACTCGTGG TGCTGGGCAC TGCTGCTGCT
  361 GCGGCAGCTG GCAACCTGAC TTGTATCGTC GCGATCGGAA ATGAGAACAG GGGCATCTTG
  421 AGCCCCTGCG GACGGTGCCG ACAGGTGCTT CTCGATCTGC ATCCTGGGAT CAAAGCCATA
  481 GTGAAGGACA GTGATGGACA GCCGACGGCA GTTGGGATTC GTGAATTGCT GCCCTCTGGT
  541 TATGTGTGGG AGGGCTCAGG AAGCGGAGCT ACTAACTTCA GCCTGCTGAA GCAGGCTGGA
  601 GACGTGGAGG AGAACCCTGG ACCTAGATCT AAGGAGAAGA GTGCTTGTCC TAAAGATCCA
  661 GCCAAACCTC CGGCCAAGGC ACAAGTTGTG GGATGGCCAC CGGTGAGATC ATACCGGAAG
  721 AACGTGATGG TTTCCTGCCA AAAATCAAGC GGTGGCCCGG AGGCGGCGGC GTTCGTGAAG
  781 GTATCAATGG ACGGAGCACC GTACTTGAGG AAAATCGATT TGAGGATGTA TAAAGCTAGC
  841 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
  901 GGCGACGTAA ACGGGCCACA AGTTCAGCGT CCGCGGCGAG GGCGAGGGCG ATGCCACCCA
  961 ACGGGCAAGC TGACCCTGAA GTTTCATCTG CACACCGGGC AAGCTGCCCG TGCCCTGGCC
 1021 ACCTCGTGGA CCACCTTCGG CTACCGCCGT TACCTGCCTT CAGTCGCTAC TCGGAACTAC
1081 ATGGAAGGCC AGCAACGCAC CATACGATTA
//
Primer: reverse RDB16809_A8Lpb.seq
>D06157A5_A8LP_2_reverse_B04_04_ABI24.ab1
    1 CAAAAAAATA ACCCCTCACT AAAGGGACAA AAGCTGGAGC TCCACCGCGG TGGCGGCCGC
   61 TCTAGAACTA GTGGATCCGG CGCCTGCACC AGCGCCCTTG TACAGCTCGT CCATGCCATG
  121 TGTAATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG
  181 GTCTTTGCTC AGCTTGGACT GATGGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC
  241 GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCTCAAC
  301 GTTGTGGCGG ATCTTGAAGT TAGCCTTGAT GCCGTTCTTC TGCTTGTCGG CCGTGATATA
  361 GACGTTGTGG CTGTTGAAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT
  421 GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC
  481 GGCGCGGGTC TTGTAGGTAC CGTCGTCCTT GAAAGAGATG GTGCGCTCCT GGACGTAGCC
  541 TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGCTGAA
  601 GCAGGCCACG CCGTAGCCGA AGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC
  661 GGTGGTGCAG ATGAACTTCA GGGTCAGCTT GCCGTTGGTG GCATCGCCCT CGCCCTCGCC
  721 GCGGACGCTG AACTTGTGGC CGTTTACGTC GCCGTCCAGC TCGACCAGGA TGGGCACCAC
  781 CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC CATGCTAGCT TTATACATCC TCAAATCGAT
  841 TTTCCTCAAG TACGGTGCTC CGTCCATTGA TACCTTCACG AACGCCGCCG CCTCCGGGCC
  901 ACCGCTTGAT TTTTGGCAGG AAACCATCAC GTTCTTCCGG TATGATCTCA CCGGGTGGCC
  961 ATCCCACAAC TTTGTGCCTT TGACCGGAGG TTTGGCTGGA TCTTTAGACA GCACTTCTCC
 1021 TCATTAGATC TAGGTCCAGG GATCTCTTCA CGTCTCAGCC TGCTCAGCAG GCTGCAGTTA
1081 GTAGCATCTG CATCTGAGTC TACCTCATGA TA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

2019.07.12

GNP_filter3_RDBDEP_html_190431.pl