Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK348 (BSD-P2A-mAID-mClover) (#RDB16809)

A plasmid for construction of N-terminal BSD-P2A-mAID-mClover tagging donors.

Clone info. A plasmid for construction of N-terminal BSD-P2A-mAID-mClover tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Kanemaki, Masato |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16809 pMK348 (BSD-P2A-mAID-mClover) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMK348 (BSD-P2A-mAID-mClover) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16809).

Reference section:

Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16809_A8Lpp1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310)
Region: T7 pro,insert 5'(BSD,P2A,mAID)
Sequence file: RDB16809_A8Lpc.seq check
>16809_16809_B3C2F_pUC-M13_-100_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNGGN NNNGCCNNGG TTTTCCCNGT CNCGACGTTG TAAAACGACG
   61 GCCAGTGAGC GCGCGTAATA CGACTCACTA TAGGGCGAAT TGGGTACCGG GCCCCCCCTC
  121 GAGGTCGACG GTATCGATAA GCTTGATATC GCCACCATGG CCAAGCCTTT GTCTCAAGAA
  181 GAATCCACCC TCATTGAAAG AGCAACGGCT ACAATCAACA GCATCCCCAT CTCTGAAGAC
  241 TACAGCGTCG CCAGCGCAGC TCTCTCTAGC GACGGCCGCA TCTTCACTGG TGTCAATGTA
  301 TATCATTTTA CTGGGGGACC TTGTGCAGAA CTCGTGGTGC TGGGCACTGC TGCTGCTGCG
  361 GCAGCTGGCA ACCTGACTTG TATCGTCGCG ATCGGAAATG AGAACAGGGG CATCTTGAGC
  421 CCCTGCGGAC GGTGCCGACA GGTGCTTCTC GATCTGCATC CTGGGATCAA AGCCATAGTG
  481 AAGGACAGTG ATGGACAGCC GACGGCAGTT GGGATTCGTG AATTGCTGCC CTCTGGTTAT
  541 GTGTGGGAGG GCTCAGGAAG CGGAGCTACT AACTTCAGCC TGCTGAAGCA GGCTGGAGAC
  601 GTGGAGGAGA ACCCTGGACC TAGATCTAAG GAGAAGAGTG CTTGTCCTAA AGATCCAGCC
  661 AAACCTCCGG CCAAGGCACA AGTTGTGGGA TGGCCACCGG TGAGATCATA CCGGANNAAC
  721 GTGATGGTTT CCTGCCNAAA ATCAAGCGGT GGCCCGGNNG CGGCGGCGTT CGTGAANGTA
  781 TCAATGGACG GANCACCGTA CTTGANGAAA ATCGATTTGA GGANGTATAA AGCTAGCATG
  841 GNGAGCANNG GCGANGANCT GTTCACCGGG GNGGTGCCNN TCCNNNNNNA NCNNGNNNGC
901 GANNNNAACN GNCNNNNNNN TCAGCGNCCG NNGCNNGGGN NANGGNNANN NNNNNNNN
//
Primer: reverse (Pr0099)
Region: insert 3'(mClover 3')
Sequence file: RDB16809_A8Lpd.seq check
>D06157A5_A8LP_2_reverse_B04_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCNNN NNNNGNNNNN AAGCTGGANC TCCNCCGCGG TGGCGGCCGC
   61 TCTAGAACTA GTGGATCCGG CGCCTGCACC AGCGCCCTTG TACAGCTCGT CCATGCCATG
  121 TGTAATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG
  181 GTCTTTGCTC AGCTTGGACT GATGGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC
  241 GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCTCAAC
  301 GTTGTGGCGG ATCTTGAAGT TAGCCTTGAT GCCGTTCTTC TGCTTGTCGG CCGTGATATA
  361 GACGTTGTGG CTGTTGAAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT
  421 GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC
  481 GGCGCGGGTC TTGTAGGTAC CGTCGTCCTT GAAAGAGATG GTGCGCTCCT GGACGTANCC
  541 TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGCTGAA
  601 GCAGGCCACG CCGTANCCGA AGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC
  661 GGTGGTGCAG ATGAACTTCA GGGTCAGCTT GCCGTTGGTG GCATCGCCCT CGCCCTCGCC
  721 GCGGANGCTG AACTTGTGGN CGTTTACGTC GCCGTCCAGC TCGACCAGGA NGGGCACCAC
  781 CCCNGTGAAC AGCTCCTCGC CCTTGCTCAC CATGCTAGCT TTATACATCN NCAAATCGAT
  841 TTTCCTCAAG TACNGNGCTN CNNNCATTGA TACNTTCACG ANCGCCNCCN CNNNCNGGCC
901 NNCNNTNNAT TTTTGNNNNN AAANCATCAC NNNCTTNCNN NATNANNNCN NNNNNNNNN
//
Primer: bsd_f1 (Pr0880)
Region: insert mid(P2A,mAID,mClover 5')
Sequence file: RDB16809_A8Lpe.seq check
>16809_16809_B3C2F_bsd_f1_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNT GCTGCCCTCT NGTNNNGTGT GGGANGGCTC AGGAAGCGGA
   61 NCTACTAACT TCAGCCTGCT GAAGCAGGCT GGAGACGTGG AGGAGAACCC TGGACCTAGA
  121 TCTAAGGAGA AGAGTGCTTG TCCTAAAGAT CCAGCCAAAC CTCCGGCCAA GGCACAAGTT
  181 GTGGGATGGC CACCGGTGAG ATCATACCGG AAGAACGTGA TGGTTTCCTG CCAAAAATCA
  241 AGCGGTGGCC CGGAGGCGGC GGCGTTCGTG AAGGTATCAA TGGACGGAGC ACCGTACTTG
  301 AGGAAAATCG ATTTGAGGAT GTATAAAGCT AGCATGGTGA GCAAGGGCGA GGAGCTGTTC
  361 ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC
  421 GTCCGCGGCG AGGGCGAGGG CGATGCCACC AACGGCAAGC TGACCCTGAA GTTCATCTGC
  481 ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCTTCGG CTACGGCGTG
  541 GCCTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG
  601 CCCGAAGGCT ACGTCCAGGA GCGCACCATC TCTTTCAAGG ACGACGGTAC CTACAAGACC
  661 CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAANGGCATC
  721 GACTTCAAGG ANGANGGCAA CATCCTGNGG CACAAGCTGG ANTACAACTT CAACAGCCAC
  781 AACGTCTATA TCACGGCCGA NAAGCAGAAG AACGGCATCN AGGCTAACTT CAAGATNCGC
  841 CACAACGTTN NNGANGGCAG CGNGCANCTC GCCNANCACT ANNNGCAGAA NCACCCCNNT
  901 CGNNNANNGC CCNNNGCTGC TGCCCNANNN NNNCTANNNN NNNCNNTCAN NNCNNNNNNN
961 ANNNNNNNCC NNNNNNNNNN
//
Primer: M13ori_R1 (Pr1087)
Region: f1 ori,AmpR pro,AmpR
Sequence file: RDB16809_A8Lpf.seq check
>16809_16809_B3C2F_M13ori_R1_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNGCG NNNNNNNNNG GNNNAAAGCG AAAGGAGCGG GCGCTAGGGC
   61 GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC
  121 GCTACAGGGC GCGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT
  181 ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT
  241 TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC
  301 CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA
  361 AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG
  421 TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT
  481 TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG
  541 CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC
  601 GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC
  661 GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA
  721 CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GANCTGAATG AAGCCATACC
  781 AAACGACGAG CGTGACACCA CGATGCCTGT ANCNNTGGCA ACAACGTNGC GCAAACTATT
  841 NACTGGCGAA CTACTTACTC TAGCTTNCNN GCNNNNATNN ATANACTGGN NGGAGGNGGA
  901 NANNTTGCNN NANCNCTTCN GNNCTCNGNC NNNNCNNNCT NGNNNNNTTA TTGCTGANNA
961 NNNNNGNNNN CNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]