Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK348 (BSD-P2A-mAID-mClover)

A plasmid for construction of N-terminal BSD-P2A-mAID-mClover tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16809_A8Lpp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310) Region: T7 pro,insert 5'(BSD,P2A,mAID) Sequence file: RDB16809_A8Lpc.seq
>16809_16809_B3C2F_pUC-M13_-100_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNGGN NNNGCCNNGG TTTTCCCNGT CNCGACGTTG TAAAACGACG    61 GCCAGTGAGC GCGCGTAATA CGACTCACTA TAGGGCGAAT TGGGTACCGG GCCCCCCCTC   121 GAGGTCGACG GTATCGATAA GCTTGATATC GCCACCATGG CCAAGCCTTT GTCTCAAGAA   181 GAATCCACCC TCATTGAAAG AGCAACGGCT ACAATCAACA GCATCCCCAT CTCTGAAGAC   241 TACAGCGTCG CCAGCGCAGC TCTCTCTAGC GACGGCCGCA TCTTCACTGG TGTCAATGTA   301 TATCATTTTA CTGGGGGACC TTGTGCAGAA CTCGTGGTGC TGGGCACTGC TGCTGCTGCG   361 GCAGCTGGCA ACCTGACTTG TATCGTCGCG ATCGGAAATG AGAACAGGGG CATCTTGAGC   421 CCCTGCGGAC GGTGCCGACA GGTGCTTCTC GATCTGCATC CTGGGATCAA AGCCATAGTG   481 AAGGACAGTG ATGGACAGCC GACGGCAGTT GGGATTCGTG AATTGCTGCC CTCTGGTTAT   541 GTGTGGGAGG GCTCAGGAAG CGGAGCTACT AACTTCAGCC TGCTGAAGCA GGCTGGAGAC   601 GTGGAGGAGA ACCCTGGACC TAGATCTAAG GAGAAGAGTG CTTGTCCTAA AGATCCAGCC   661 AAACCTCCGG CCAAGGCACA AGTTGTGGGA TGGCCACCGG TGAGATCATA CCGGANNAAC   721 GTGATGGTTT CCTGCCNAAA ATCAAGCGGT GGCCCGGNNG CGGCGGCGTT CGTGAANGTA   781 TCAATGGACG GANCACCGTA CTTGANGAAA ATCGATTTGA GGANGTATAA AGCTAGCATG   841 GNGAGCANNG GCGANGANCT GTTCACCGGG GNGGTGCCNN TCCNNNNNNA NCNNGNNNGC 901 GANNNNAACN GNCNNNNNNN TCAGCGNCCG NNGCNNGGGN NANGGNNANN NNNNNNNN //
Primer: reverse (Pr0099) Region: insert 3'(mClover 3') Sequence file: RDB16809_A8Lpd.seq
>D06157A5_A8LP_2_reverse_B04_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNCNNN NNNNGNNNNN AAGCTGGANC TCCNCCGCGG TGGCGGCCGC    61 TCTAGAACTA GTGGATCCGG CGCCTGCACC AGCGCCCTTG TACAGCTCGT CCATGCCATG   121 TGTAATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG   181 GTCTTTGCTC AGCTTGGACT GATGGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC   241 GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCTCAAC   301 GTTGTGGCGG ATCTTGAAGT TAGCCTTGAT GCCGTTCTTC TGCTTGTCGG CCGTGATATA   361 GACGTTGTGG CTGTTGAAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT   421 GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC   481 GGCGCGGGTC TTGTAGGTAC CGTCGTCCTT GAAAGAGATG GTGCGCTCCT GGACGTANCC   541 TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGCTGAA   601 GCAGGCCACG CCGTANCCGA AGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC   661 GGTGGTGCAG ATGAACTTCA GGGTCAGCTT GCCGTTGGTG GCATCGCCCT CGCCCTCGCC   721 GCGGANGCTG AACTTGTGGN CGTTTACGTC GCCGTCCAGC TCGACCAGGA NGGGCACCAC   781 CCCNGTGAAC AGCTCCTCGC CCTTGCTCAC CATGCTAGCT TTATACATCN NCAAATCGAT   841 TTTCCTCAAG TACNGNGCTN CNNNCATTGA TACNTTCACG ANCGCCNCCN CNNNCNGGCC 901 NNCNNTNNAT TTTTGNNNNN AAANCATCAC NNNCTTNCNN NATNANNNCN NNNNNNNNN //
Primer: bsd_f1 (Pr0880) Region: insert mid(P2A,mAID,mClover 5') Sequence file: RDB16809_A8Lpe.seq
>16809_16809_B3C2F_bsd_f1_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNT GCTGCCCTCT NGTNNNGTGT GGGANGGCTC AGGAAGCGGA    61 NCTACTAACT TCAGCCTGCT GAAGCAGGCT GGAGACGTGG AGGAGAACCC TGGACCTAGA   121 TCTAAGGAGA AGAGTGCTTG TCCTAAAGAT CCAGCCAAAC CTCCGGCCAA GGCACAAGTT   181 GTGGGATGGC CACCGGTGAG ATCATACCGG AAGAACGTGA TGGTTTCCTG CCAAAAATCA   241 AGCGGTGGCC CGGAGGCGGC GGCGTTCGTG AAGGTATCAA TGGACGGAGC ACCGTACTTG   301 AGGAAAATCG ATTTGAGGAT GTATAAAGCT AGCATGGTGA GCAAGGGCGA GGAGCTGTTC   361 ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC   421 GTCCGCGGCG AGGGCGAGGG CGATGCCACC AACGGCAAGC TGACCCTGAA GTTCATCTGC   481 ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCTTCGG CTACGGCGTG   541 GCCTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG   601 CCCGAAGGCT ACGTCCAGGA GCGCACCATC TCTTTCAAGG ACGACGGTAC CTACAAGACC   661 CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAANGGCATC   721 GACTTCAAGG ANGANGGCAA CATCCTGNGG CACAAGCTGG ANTACAACTT CAACAGCCAC   781 AACGTCTATA TCACGGCCGA NAAGCAGAAG AACGGCATCN AGGCTAACTT CAAGATNCGC   841 CACAACGTTN NNGANGGCAG CGNGCANCTC GCCNANCACT ANNNGCAGAA NCACCCCNNT   901 CGNNNANNGC CCNNNGCTGC TGCCCNANNN NNNCTANNNN NNNCNNTCAN NNCNNNNNNN 961 ANNNNNNNCC NNNNNNNNNN //
Primer: M13ori_R1 (Pr1087) Region: f1 ori,AmpR pro,AmpR Sequence file: RDB16809_A8Lpf.seq
>16809_16809_B3C2F_M13ori_R1_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNGCG NNNNNNNNNG GNNNAAAGCG AAAGGAGCGG GCGCTAGGGC    61 GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC   121 GCTACAGGGC GCGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT   181 ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT   241 TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC   301 CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA   361 AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG   421 TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT   481 TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG   541 CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC   601 GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC   661 GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA   721 CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GANCTGAATG AAGCCATACC   781 AAACGACGAG CGTGACACCA CGATGCCTGT ANCNNTGGCA ACAACGTNGC GCAAACTATT   841 NACTGGCGAA CTACTTACTC TAGCTTNCNN GCNNNNATNN ATANACTGGN NGGAGGNGGA   901 NANNTTGCNN NANCNCTTCN GNNCTCNGNC NNNNCNNNCT NGNNNNNTTA TTGCTGANNA 961 NNNNNGNNNN CNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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