Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK348 (BSD-P2A-mAID-mClover)

A plasmid for construction of N-terminal BSD-P2A-mAID-mClover tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16809_A8Lpp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 Region: T7 pro, BSD, P2A, mAID, mClover Sequence file: RDB16809_A8Lpa.seq
>D06157A5_A8LP_2_pUC-M13_-100_A04_01_ABI24.ab1     1 GGCGGTCAAG CGGATTAGTT GGGTACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG    61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGGTAC CGGGCCCCCC   121 CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGCCACCA TGGCCAAGCC TTTGTCTCAA   181 GAAGAATCCA CCCTCATTGA AAGAGCAACG GCTACAATCA ACAGCATCCC CATCTCTGAA   241 GACTACAGCG TCGCCAGCGC AGCTCTCTCT AGCGACGGCC GCATCTTCAC TGGTGTCAAT   301 GTATATCATT TTACTGGGGG ACCTTGTGCA GAACTCGTGG TGCTGGGCAC TGCTGCTGCT   361 GCGGCAGCTG GCAACCTGAC TTGTATCGTC GCGATCGGAA ATGAGAACAG GGGCATCTTG   421 AGCCCCTGCG GACGGTGCCG ACAGGTGCTT CTCGATCTGC ATCCTGGGAT CAAAGCCATA   481 GTGAAGGACA GTGATGGACA GCCGACGGCA GTTGGGATTC GTGAATTGCT GCCCTCTGGT   541 TATGTGTGGG AGGGCTCAGG AAGCGGAGCT ACTAACTTCA GCCTGCTGAA GCAGGCTGGA   601 GACGTGGAGG AGAACCCTGG ACCTAGATCT AAGGAGAAGA GTGCTTGTCC TAAAGATCCA   661 GCCAAACCTC CGGCCAAGGC ACAAGTTGTG GGATGGCCAC CGGTGAGATC ATACCGGAAG   721 AACGTGATGG TTTCCTGCCA AAAATCAAGC GGTGGCCCGG AGGCGGCGGC GTTCGTGAAG   781 GTATCAATGG ACGGAGCACC GTACTTGAGG AAAATCGATT TGAGGATGTA TAAAGCTAGC   841 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC   901 GGCGACGTAA ACGGGCCACA AGTTCAGCGT CCGCGGCGAG GGCGAGGGCG ATGCCACCCA   961 ACGGGCAAGC TGACCCTGAA GTTTCATCTG CACACCGGGC AAGCTGCCCG TGCCCTGGCC  1021 ACCTCGTGGA CCACCTTCGG CTACCGCCGT TACCTGCCTT CAGTCGCTAC TCGGAACTAC 1081 ATGGAAGGCC AGCAACGCAC CATACGATTA //
Primer: reverse Region: Linker, mClover, mAID Sequence file: RDB16809_A8Lpb.seq
>D06157A5_A8LP_2_reverse_B04_04_ABI24.ab1     1 CAAAAAAATA ACCCCTCACT AAAGGGACAA AAGCTGGAGC TCCACCGCGG TGGCGGCCGC    61 TCTAGAACTA GTGGATCCGG CGCCTGCACC AGCGCCCTTG TACAGCTCGT CCATGCCATG   121 TGTAATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG   181 GTCTTTGCTC AGCTTGGACT GATGGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC   241 GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCTCAAC   301 GTTGTGGCGG ATCTTGAAGT TAGCCTTGAT GCCGTTCTTC TGCTTGTCGG CCGTGATATA   361 GACGTTGTGG CTGTTGAAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT   421 GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC   481 GGCGCGGGTC TTGTAGGTAC CGTCGTCCTT GAAAGAGATG GTGCGCTCCT GGACGTAGCC   541 TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGCTGAA   601 GCAGGCCACG CCGTAGCCGA AGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC   661 GGTGGTGCAG ATGAACTTCA GGGTCAGCTT GCCGTTGGTG GCATCGCCCT CGCCCTCGCC   721 GCGGACGCTG AACTTGTGGC CGTTTACGTC GCCGTCCAGC TCGACCAGGA TGGGCACCAC   781 CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC CATGCTAGCT TTATACATCC TCAAATCGAT   841 TTTCCTCAAG TACGGTGCTC CGTCCATTGA TACCTTCACG AACGCCGCCG CCTCCGGGCC   901 ACCGCTTGAT TTTTGGCAGG AAACCATCAC GTTCTTCCGG TATGATCTCA CCGGGTGGCC   961 ATCCCACAAC TTTGTGCCTT TGACCGGAGG TTTGGCTGGA TCTTTAGACA GCACTTCTCC  1021 TCATTAGATC TAGGTCCAGG GATCTCTTCA CGTCTCAGCC TGCTCAGCAG GCTGCAGTTA 1081 GTAGCATCTG CATCTGAGTC TACCTCATGA TA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2021.11.02

GNP_filter3_RDBDEP_html_211029.pl