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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK348 (BSD-P2A-mAID-mClover)

A plasmid for construction of N-terminal BSD-P2A-mAID-mClover tagging donors.

Catalog number RDB16809
Resource name pMK348 (BSD-P2A-mAID-mClover)
Clone info. A plasmid for construction of N-terminal BSD-P2A-mAID-mClover tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank

External Database
Arabidopsis thaliana AtIAA17 Aequorea victoria GFP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Schedule A (GFP-A) [Link]
          Remarks
          Please use Schedule A (GFP-A) additionally.
          Distribution information (domestic users) [open/close]


          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16809 pMK348 (BSD-P2A-mAID-mClover) DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16809_A8Lpp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pUC-M13_-100
          Region: T7 pro, BSD, P2A, mAID, mClover
          Sequence file: RDB16809_A8Lpa.seq check
          >D06157A5_A8LP_2_pUC-M13_-100_A04_01_ABI24.ab1
              1 GGCGGTCAAG CGGATTAGTT GGGTACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG
             61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGGTAC CGGGCCCCCC
            121 CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGCCACCA TGGCCAAGCC TTTGTCTCAA
            181 GAAGAATCCA CCCTCATTGA AAGAGCAACG GCTACAATCA ACAGCATCCC CATCTCTGAA
            241 GACTACAGCG TCGCCAGCGC AGCTCTCTCT AGCGACGGCC GCATCTTCAC TGGTGTCAAT
            301 GTATATCATT TTACTGGGGG ACCTTGTGCA GAACTCGTGG TGCTGGGCAC TGCTGCTGCT
            361 GCGGCAGCTG GCAACCTGAC TTGTATCGTC GCGATCGGAA ATGAGAACAG GGGCATCTTG
            421 AGCCCCTGCG GACGGTGCCG ACAGGTGCTT CTCGATCTGC ATCCTGGGAT CAAAGCCATA
            481 GTGAAGGACA GTGATGGACA GCCGACGGCA GTTGGGATTC GTGAATTGCT GCCCTCTGGT
            541 TATGTGTGGG AGGGCTCAGG AAGCGGAGCT ACTAACTTCA GCCTGCTGAA GCAGGCTGGA
            601 GACGTGGAGG AGAACCCTGG ACCTAGATCT AAGGAGAAGA GTGCTTGTCC TAAAGATCCA
            661 GCCAAACCTC CGGCCAAGGC ACAAGTTGTG GGATGGCCAC CGGTGAGATC ATACCGGAAG
            721 AACGTGATGG TTTCCTGCCA AAAATCAAGC GGTGGCCCGG AGGCGGCGGC GTTCGTGAAG
            781 GTATCAATGG ACGGAGCACC GTACTTGAGG AAAATCGATT TGAGGATGTA TAAAGCTAGC
            841 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
            901 GGCGACGTAA ACGGGCCACA AGTTCAGCGT CCGCGGCGAG GGCGAGGGCG ATGCCACCCA
            961 ACGGGCAAGC TGACCCTGAA GTTTCATCTG CACACCGGGC AAGCTGCCCG TGCCCTGGCC
           1021 ACCTCGTGGA CCACCTTCGG CTACCGCCGT TACCTGCCTT CAGTCGCTAC TCGGAACTAC
          1081 ATGGAAGGCC AGCAACGCAC CATACGATTA
          //
          Primer: reverse
          Region: Linker, mClover, mAID
          Sequence file: RDB16809_A8Lpb.seq check
          >D06157A5_A8LP_2_reverse_B04_04_ABI24.ab1
              1 CAAAAAAATA ACCCCTCACT AAAGGGACAA AAGCTGGAGC TCCACCGCGG TGGCGGCCGC
             61 TCTAGAACTA GTGGATCCGG CGCCTGCACC AGCGCCCTTG TACAGCTCGT CCATGCCATG
            121 TGTAATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG
            181 GTCTTTGCTC AGCTTGGACT GATGGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC
            241 GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCTCAAC
            301 GTTGTGGCGG ATCTTGAAGT TAGCCTTGAT GCCGTTCTTC TGCTTGTCGG CCGTGATATA
            361 GACGTTGTGG CTGTTGAAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT
            421 GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC
            481 GGCGCGGGTC TTGTAGGTAC CGTCGTCCTT GAAAGAGATG GTGCGCTCCT GGACGTAGCC
            541 TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGCTGAA
            601 GCAGGCCACG CCGTAGCCGA AGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC
            661 GGTGGTGCAG ATGAACTTCA GGGTCAGCTT GCCGTTGGTG GCATCGCCCT CGCCCTCGCC
            721 GCGGACGCTG AACTTGTGGC CGTTTACGTC GCCGTCCAGC TCGACCAGGA TGGGCACCAC
            781 CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC CATGCTAGCT TTATACATCC TCAAATCGAT
            841 TTTCCTCAAG TACGGTGCTC CGTCCATTGA TACCTTCACG AACGCCGCCG CCTCCGGGCC
            901 ACCGCTTGAT TTTTGGCAGG AAACCATCAC GTTCTTCCGG TATGATCTCA CCGGGTGGCC
            961 ATCCCACAAC TTTGTGCCTT TGACCGGAGG TTTGGCTGGA TCTTTAGACA GCACTTCTCC
           1021 TCATTAGATC TAGGTCCAGG GATCTCTTCA CGTCTCAGCC TGCTCAGCAG GCTGCAGTTA
          1081 GTAGCATCTG CATCTGAGTC TACCTCATGA TA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

          2021.11.02

          GNP_filter3_RDBDEP_html_211029.pl