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pMK392 (mAID-mClover-BSD) (#RDB16820)

A plasmid for construction of C-terminal mAID-mClover-BSD tagging donors.

Clone info. A plasmid for construction of C-terminal mAID-mClover-BSD tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Kanemaki, Masato |
 
Sequence (full) RDB16820hts01.seq checkAssembled from experimentally sequenced data.
Publication Yesbolatova, A., Methods pii: S1046-2023 (18) 30331-1 (2019). [link to RRC of NBRP]
Test sheet RDB16820_B0Ghp1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16820 pMK392 (mAID-mClover-BSD) DNA solution


How to cite this biological resource

Materials & Methods section:

The pMK392 (mAID-mClover-BSD) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16820).

Reference section:

Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]