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pMK392 (mAID-mClover-BSD)

A plasmid for construction of C-terminal mAID-mClover-BSD tagging donors.

Catalog number RDB16820
Resource name pMK392 (mAID-mClover-BSD)
Clone info. A plasmid for construction of C-terminal mAID-mClover-BSD tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Aequorea victoria GFP cDNA
Depositor Kanemaki, Masato |
 
Other clones in our bank Arabidopsis thaliana AXR3 (KEGG orthology K14484) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering Please visit Information of Request for Distribution. 
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16820 pMK392 (mAID-mClover-BSD) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16820_A8Lpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 RDB16820_A8Lpa.seq
>D06157B7_A8Lp_1_pUC-M13_-100_A07_01_ABI24.ab1
    1 GGCCGGTCAA GCGGAAAAGT TGGGTACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC
   61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGAGC TCCCCGGGAT
  121 CCGGTGCAGG CGCCAAGGAG AAGAGTGCTT GTCCTAAAGA TCCAGCCAAA CCTCCGGCCA
  181 AGGCACAAGT TGTGGGATGG CCACCGGTGA GATCATACCG GAAGAACGTG ATGGTTTCCT
  241 GCCAAAAATC AAGCGGTGGC CCGGAGGCGG CGGCGTTCGT GAAGGTATCA ATGGACGGAG
  301 CACCGTACTT GAGGAAAATC GATTTGAGGA TGTATAAAGC TAGCATGGTG AGCAAGGGCG
  361 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC
  421 ACAAGTTCAG CGTCCGCGGC GAGGGCGAGG GCGATGCCAC CAACGGCAAG CTGACCCTGA
  481 AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCTTCG
  541 GCTACGGCGT GGCCTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA
  601 AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTCTTTCAAG GACGACGGTA
  661 CCTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC
  721 TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT
  781 TCAACAGCCA CAACGTCTAT ATCACGGCCG ACAAGCAGAA GAACGGCATC AAGGCTAACT
  841 TCAAGATCCG CCACAACGTT GAGGGACGGC AGCGTGCAGC TCGCCGACCA CCTACCAGCA
  901 GAACACCCCC CATCGGCGAC GGCCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCCAT
  961 CAGTCCCAAG CTGAGCCAAA GACCCCCAAC GAGAAGCCGG CGAATCACAA TGGGTCCTTG
 1021 CCTGGGAAGT TTCGTTGACC GGCCGGCCCG GGAATTACCC ATTGGCAATG GGACGGAGCT
1081 GGTCCATTTG TGGGCGCGTG AATTCATAAA TCCCGATCCC ATTAGCA
//
Primer: reverse RDB16820_A8Lpb.seq
>D06157B7_A8Lp_1_reverse_B07_04_ABI24.ab1
    1 CGGGCAATTA CCTCACTAAA GGGAACAAAG CTGGGTACCG GATCCTAGTG AACCTCTTCG
   61 AGGGACCTAA TAACTTCGTA TAGCATACAT TATACGAAGT TATATTAAGG GTTCCGGATC
  121 TCGACCAGCT TCTGATGGAA TTAGAACTTG GCAAAACAAT ACTGAGAATG AAGTGTATGT
  181 GGAACAGAGG CTGCTGATCT CGTTCTTCAG GCTATGAAAC TGACACATTT GGAAACCACA
  241 GTACTTAGAA CCACAAAGTG GGAATCAAGA GAAAAACAAT GATCCCACGA GAGATCTATA
  301 GATCTATAGA TCATGAGTGG GAGGAATGAG CTGGCCCTTA ATTTGGTTTT GCTTGTTTAA
  361 ATTATGATAT CCAACTATGA AACATTATCA TAAAGCAATA GTAAAGAGCC TTCAGTAAAG
  421 AGCAGGCATT TATCTAATCC CACCCCACCC CCACCCCCGT AGCTCCAATC CTTCCATTCA
  481 AAATGTAGGT ACTCTGTTCT CACCCTTCTT AACAAAGTAT GACAGGAAAA ACTTCCATTT
  541 TAGTGGACAT CTTTATTGTT TAATAGATCA TCAATTTCTG CATCCCGGGG ATCTGATATC
  601 ATCGATTAGC CCTCCCACAC ATAACCAGAG GGCAGCAATT CACGAATCCC AACTGCCGTC
  661 GGCTGTCCAT CACTGTCCTT CACTATGGCT TTGATCCCAG GATGCAGATC GAGAAGCACC
  721 TGTCGGCACC GTCCGCAGGG GCTCAAGATG CCCCTGTTCT CATTTCCGAT CGCGACGATA
  781 CAAGTCAGGT TGCCAGCTGC CGCAGCAGCA GCAGTGCCCA GCACCACGAG TTCTGCACAA
  841 GGTCCCCCCA GTAAAATGAT ATACATTGAC ACCAGTGAAG ATGCGGCCGT CGCTAGAGAG
  901 AGCTGCGCTG GCGACGCTGT AGTCTTCAGA GATGGGGATG CTGTTGATTG TAGCCGTTGC
  961 TCTTTCAATG AGGGTGGGAT TCTTCTTGAG ACAAAGGCTT GGACCAATGG GTGGGCTCGA
 1021 GATCCTAGAT GGATGCAGGT CGAAAGGCCC CGAGATGAGG AAGAGAAGAA CCAGCGCCGG
1081 TCCAGAACCG GATGC
//
Primer: PGK_N_rev RDB16820_A8Lpc.seq
>D06157B7_A8Lp_1_PGK_N_rev_C07_07_ABI24.ab1
    1 CGTATGGGCC GCCTCCCTAC CCGGTAGATT TCGAGGTCGA GATCCTAAGC TTGGCTGGAC
   61 GTAAACTCCT CTTCAGACCT AATAACTTCG TATAGCATAC ATTATACGAA GTTATATTAA
  121 GGGTTATTGA ATATGATCGA CGCGTTAAGA TACATTGATG AGTTTGGACA AACCACAACT
  181 AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA
  241 ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG
  301 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG
  361 GCTGATTATG ATCAGTTATC TAGATTACTT GTACAGCTCG TCCATGCCAT GTGTAATCCC
  421 GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT
  481 CAGCTTGGAC TGATGGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT
  541 GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCAA CGTTGTGGCG
  601 GATCTTGAAG TTAGCCTTGA TGCCGTTCTT CTGCTTGTCG GCCGTGATAT AGACGTTGTG
  661 GCTGTTGAAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT
  721 GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT
  781 CTTGTAGGTA CCGTCGTCCT TGAAAGAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT
  841 GGCGGACTTG AAAGAAGTCG TGCTGCTTCA TGTGGTCTGG GTAGCGGCTG AAGCAGCCAC
  901 GCCGTAGCCG AAATGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GGTGGTGCAG
  961 ATGAACTTCA GGGTCAGCTT GCCGTTGGTG GCATCGCCCT CGCCCTCGCC GCGACGCTGG
 1021 AACTTGTGAC GGTTACGTCG CCGGTCCAGC TCGACCAGAA TGGCACCACC CTGTGGAAAC
1081 AGCTCCT
//
Primer: PGKpro_F RDB16820_A8Lpd.seq
>D06157B7_A8Lp_1_PGKpro_F_D07_10_ABI24.ab1
    1 CTCGGGGCCG ATTCTCTCTT CTCATCTCCG GGCCTTTCGA CCTGCATCCA TCTAGATCTC
   61 GAGCCACCAT GGCCAAGCCT TTGTCTCAAG AAGAATCCAC CCTCATTGAA AGAGCAACGG
  121 CTACAATCAA CAGCATCCCC ATCTCTGAAG ACTACAGCGT CGCCAGCGCA GCTCTCTCTA
  181 GCGACGGCCG CATCTTCACT GGTGTCAATG TATATCATTT TACTGGGGGA CCTTGTGCAG
  241 AACTCGTGGT GCTGGGCACT GCTGCTGCTG CGGCAGCTGG CAACCTGACT TGTATCGTCG
  301 CGATCGGAAA TGAGAACAGG GGCATCTTGA GCCCCTGCGG ACGGTGCCGA CAGGTGCTTC
  361 TCGATCTGCA TCCTGGGATC AAAGCCATAG TGAAGGACAG TGATGGACAG CCGACGGCAG
  421 TTGGGATTCG TGAATTGCTG CCCTCTGGTT ATGTGTGGGA GGGCTAATCG ATGATATCAG
  481 ATCCCCGGGA TGCAGAAATT GATGATCTAT TAAACAATAA AGATGTCCAC TAAAATGGAA
  541 GTTTTTCCTG TCATACTTTG TTAAGAAGGG TGAGAACAGA GTACCTACAT TTTGAATGGA
  601 AGGATTGGAG CTACGGGGGT GGGGGTGGGG TGGGATTAGA TAAATGCCTG CTCTTTACTG
  661 AAGGCTCTTT ACTATTGCTT TATGATAATG TTTCATAGTT GGATATCATA ATTTAAACAA
721 GCAAAACCAA ATTAAGGGCC AGCTCATTC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

2019.07.12

GNP_filter3_RDBDEP_html_190431.pl