Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK392 (mAID-mClover-BSD)

A plasmid for construction of C-terminal mAID-mClover-BSD tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

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Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16820_A8Lpp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310) Region: T7 pro,insert 5'(Linker,mAID, mClover 5') Sequence file: RDB16820_A8Lpd.seq
>D06157B7_A8Lp_1_pUC-M13_-100_A07_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NGGGNNNGCN NGGNNTTTCC CNGTNNCGAC GTTGTAAAAC    61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGAGC TCCCCGGGAT   121 CCGGTGCAGG CGCCAAGGAG AAGAGTGCTT GTCCTAAAGA TCCAGCCAAA CCTCCGGCCA   181 AGGCACAAGT TGTGGGATGG CCACCGGTGA GATCATACCG GAAGAACGTG ATGGTTTCCT   241 GCCAAAAATC AAGCGGTGGC CCGGAGGCGG CGGCGTTCGT GAAGGTATCA ATGGACGGAG   301 CACCGTACTT GAGGAAAATC GATTTGAGGA TGTATAAAGC TAGCATGGTG AGCAAGGGCG   361 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC   421 ACAAGTTCAG CGTCCGCGGC GAGGGCGAGG GCGATGCCAC CAACGGCAAG CTGACCCTGA   481 AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCTTCG   541 GCTACGGCGT GGCCTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA   601 AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTCTTTCAAG GACGACGNNN   661 NNNACAAGAC CCGCGCCGAN GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC   721 TGAANGGCAT CNNNTTCAAG GAGGANGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT   781 TCAACAGCCA CAACGTCTAT ATCACGGCCG ANAAGCANAA NAANGGCATC NANGCTAANT   841 TNNAGATCCG CCACAACGTT NNNGGANNGN ANCGTGCAGC TCGCCNANCA NNTACNANCA   901 NAACNCCCCC NNTCGNNGAN NGNCCCCGTG CNNCTGCCCG ANNNNNNCNA CCTGAGCNNT 961 NNNNNCNNNN CTGNNNNNAN NNNNNCNNNN NANNNNNNNN NN //
Primer: lacIpro-B_F (Pr0724) Region: T3 pro,insert 3'(loxP,mPGK ter 3') Sequence file: RDB16820_A8Lpe.seq
>16820_16820_B3C9F_lacIpro-B_F_H02_23_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN CGNANCNNNG TCNGTGAGCG AGGAANNCNN GAAGAGCGCC    61 CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA   121 GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC   181 ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA   241 GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG CGCGCAATTA   301 ACCCTCACTA AAGGGAACAA AAGCTGGGTA CCGGATCCTA GTGAACCTCT TCGAGGGACC   361 TAATAACTTC GTATAGCATA CATTATACGA AGTTATATTA AGGGTTCCGG ATCTCGACCA   421 GCTTCTGATG GAATTAGAAC TTGGCAAAAC AATACTGAGA ATGAAGTGTA TGTGGAACAG   481 AGGCTGCTGA TCTCGTTCTT CAGGCTATGA AACTGACACA TTTGGAAACC ACAGTACTTA   541 GAACCACAAA GTGGGAATCA AGAGAAAAAC AATGATCCNN CNAGAGATCT ATAGATCTAT   601 AGATCATGAG TGNGAGGAAT GAGCTGGNCC TTAATTTGNN TTTGCTNGNT TAAATTATGA   661 TATCCAACTA TGAAANATTA TCATAAAGCN ATAGTAAAGA GCNNTCANTA AAGANNNNNN   721 ATTTATCNNA TCCCNCCCCA CCCCCNCCCC NNNANCTNCN NTNNNTNNAT TNAAATGNNN   781 GNNACTCNNN NNNNNNCNNN NNTNANAANN NNGNNAGNAA AAANNTNNNT TTTNNNGNNN 841 NNNNTNNNTG NTNANNNNNA TNNTNNNNTN NNNNNNNCNN GGNNNNNNNN NN //
Primer: PGK_N_rev (Pr0243) Region: insert mid(loxP,SV40 pA,mClover 3') Sequence file: RDB16820_A8Lpf.seq
>D06157B7_A8Lp_1_PGK_N_rev_C07_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGGNNNANT TCGAGGTCGA GNTCCTAAGC TTGGCTGGAC    61 GTAAACTCCT CTTCAGACCT AATAACTTCG TATAGCATAC ATTATACGAA GTTATATTAA   121 GGGTTATTGA ATATGATCGA CGCGTTAAGA TACATTGATG AGTTTGGACA AACCACAACT   181 AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA   241 ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG   301 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG   361 GCTGATTATG ATCAGTTATC TAGATTACTT GTACAGCTCG TCCATGCCAT GTGTAATCCC   421 GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT   481 CAGCTTGGAC TGATGGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT   541 GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCAA CGTTGTGGCG   601 GATCTTGAAG TTAGCCTTGA TGCCGTTCTT CTGCTTGTCG GCCGTGATAT AGACGTTGTG   661 GCTGTTGAAG TTGTACTCCA GCTTGTGCCC CANGATGTTG CCGTCCTCCT TGAAGTCGAT   721 GCCCTTCAGC TCGATGCGGT TCANCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT   781 CTTGTANGTA CCGTCNTCCT TGAAAGANAT GGTGCGCTCC TGGACGTAGC CTTCNGGCAT   841 GGCGGANTTG NAANAAGTCN NGCTGCTNNA TGTGGTNNNG GNANCGGCTN AANCNNNNNN 901 NCNNNNNNCN ANNNNNNCAC NAGGGNGGGN NAGGGNANGG GCAGCTNNNN NNNNNN //
Primer: PGKpro_F (Pr0249) Region: insert mid(BSD,mPGK ter 5') Sequence file: RDB16820_A8Lpg.seq
>D06157B7_A8Lp_1_PGKpro_F_D07_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNTCTCCN GGCCTTTCGN CCTGCATCCN TCTAGATCTC    61 GAGCCACCAT GGCCAAGCCT TTGTCTCAAG AAGAATCCAC CCTCATTGAA AGAGCAACGG   121 CTACAATCAA CAGCATCCCC ATCTCTGAAG ACTACAGCGT CGCCAGCGCA GCTCTCTCTA   181 GCGACGGCCG CATCTTCACT GGTGTCAATG TATATCATTT TACTGGGGGA CCTTGTGCAG   241 AACTCGTGGT GCTGGGCACT GCTGCTGCTG CGGCAGCTGG CAACCTGACT TGTATCGTCG   301 CGATCGGAAA TGAGAACAGG GGCATCTTGA GCCCCTGCGG ACGGTGCCGA CAGGTGCTTC   361 TCGATCTGCA TCCTGGGATC AAAGCCATAG TGAAGGACAG TGATGGACAG CCGACGGCAG   421 TTGGGATTCG TGAATTGCTG CCCTCTGGTT ATGTGTGGGA GGGCTAATCG ATGATATCAG   481 ATCCCCGGGA TGCAGAAATT GATGATCTAT TAAACAATAA AGATGTCCAC TAAAATGGAA   541 GTTTTTCCTG TCATACTTTG TTAAGAAGGG TGAGAACAGA GTACCTACAT TTTGAATGGA   601 AGGATTGGAG CTACGGGGGT GGGGGTGGGG TGGGATTAGA TAAATGCCTG CTCTTTACTG   661 AAGGCTCTTT ACTATTGCTT TATGATAATG TTTCATAGTT GGATATCATA ATTTAAACAA   721 GCAAAACCAA ATTAAGGGCN AGCTCATTCC TCCCACTCAT GATCTATAGA TCTATAGNTC   781 TCTCGTGNNA TCATTGTTTT TCTCTTGATT CCCACTTTGT GGTTCTNAGT ACTGTGGTTT   841 CNNAATGTGT CAGTTTCNTA NCNTGNAGAA CGANNTCAGC AGCCTCTGTN CCNCATNCAC   901 TTCATTCTCA NNATTNNTTT GCCNAGTNNN NNNTCNNTCA NAANCTNGNN NNNNATNNNG 961 NNNNNTNANN ANNNNNTNNN NNNNNNN //
Primer: xFPendR2 (Pr1209) Region: insert mid(mAID 3',mClover 5') Sequence file: RDB16820_A8Lph.seq
>16820_16820_B3C9F_xFPendR2_H03_24_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GNNNNTGTGN NCGCGCTNNN NNNTGGGGTC    61 TTTGCTCAGC TNGNNNNNNN NNNGCTCAGG TAGTGGTTGT CGGGCAGCAG CACGGGGCCG   121 TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCTGCC GTCCTCAACG   181 TTGTGGCGGA TCTTGAAGTT AGCCTTGATG CCGTTCTTCT GCTTGTCGGC CGTGATATAG   241 ACGTTGTGGC TGTTGAAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC GTCCTCCTTG   301 AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA CTTCACCTCG   361 GCGCGGGTCT TGTAGGTACC GTCGTCCTTG AAAGAGATGG TGCGCTCCTG GACGTAGCCT   421 TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA GCGGCTGAAG   481 CAGGCCACGC CGTAGCCGAA GGTGGTCACG AGGGTGGGCC AGGGCACGGG CAGCTTGCCG   541 GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTTGGTGG CATCGCCCTC GCCCTCGCCG   601 CGGACGCTGA ACTTGTGGCC GTTTACGTCG CCGTCCAGCT CGACCAGGAT GGGCACCACC   661 CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATGCTAGCTT TATACATCCT CNAATCGATT   721 TTCCTCAAGT ACGGTGCTCC NTCCNTTGAT ACCTTCACGA ACGCCGCCNC CTNNNGGCCA   781 CCGCTTGNNT TTTNGNNNNN AAACCATCAC GTNCTTNCNG TATGATCTCA NCNGNGGNCN   841 NNNCCANANT TGTGCCTTGN NNNGANNNTT NGNNTGGATC TTTNNNANAN NNCTCNTCNN   901 NNTNNNGCNN GCNNCNNNNN CNGGGNNNNN TNNNNNNNNC CNNANANNGA GTCNNNNTNN 961 NCNNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl