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pMK392 (mAID-mClover-BSD)

A plasmid for construction of C-terminal mAID-mClover-BSD tagging donors.

Catalog number RDB16820
Resource name pMK392 (mAID-mClover-BSD)
Clone info. A plasmid for construction of C-terminal mAID-mClover-BSD tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank

External Database
Arabidopsis thaliana AtIAA17 Aequorea victoria GFP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Schedule A (GFP-A) [Link]
          Remarks
          Please use Schedule A (GFP-A) additionally.
          Distribution information (domestic users) [open/close]


          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16820 pMK392 (mAID-mClover-BSD) DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16820_A8Lpp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pUC-M13_-100
          Region: T7 pro, linker, insert 5' (mAID, mClover)
          Sequence file: RDB16820_A8Lpa.seq check
          >D06157B7_A8Lp_1_pUC-M13_-100_A07_01_ABI24.ab1
              1 GGCCGGTCAA GCGGAAAAGT TGGGTACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC
             61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGAGC TCCCCGGGAT
            121 CCGGTGCAGG CGCCAAGGAG AAGAGTGCTT GTCCTAAAGA TCCAGCCAAA CCTCCGGCCA
            181 AGGCACAAGT TGTGGGATGG CCACCGGTGA GATCATACCG GAAGAACGTG ATGGTTTCCT
            241 GCCAAAAATC AAGCGGTGGC CCGGAGGCGG CGGCGTTCGT GAAGGTATCA ATGGACGGAG
            301 CACCGTACTT GAGGAAAATC GATTTGAGGA TGTATAAAGC TAGCATGGTG AGCAAGGGCG
            361 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC
            421 ACAAGTTCAG CGTCCGCGGC GAGGGCGAGG GCGATGCCAC CAACGGCAAG CTGACCCTGA
            481 AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCTTCG
            541 GCTACGGCGT GGCCTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA
            601 AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTCTTTCAAG GACGACGGTA
            661 CCTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC
            721 TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT
            781 TCAACAGCCA CAACGTCTAT ATCACGGCCG ACAAGCAGAA GAACGGCATC AAGGCTAACT
            841 TCAAGATCCG CCACAACGTT GAGGGACGGC AGCGTGCAGC TCGCCGACCA CCTACCAGCA
            901 GAACACCCCC CATCGGCGAC GGCCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCCAT
            961 CAGTCCCAAG CTGAGCCAAA GACCCCCAAC GAGAAGCCGG CGAATCACAA TGGGTCCTTG
           1021 CCTGGGAAGT TTCGTTGACC GGCCGGCCCG GGAATTACCC ATTGGCAATG GGACGGAGCT
          1081 GGTCCATTTG TGGGCGCGTG AATTCATAAA TCCCGATCCC ATTAGCA
          //
          Primer: reverse
          Region: loxP-2, mouse PGK ter, insert 3' (BSD)
          Sequence file: RDB16820_A8Lpb.seq check
          >D06157B7_A8Lp_1_reverse_B07_04_ABI24.ab1
              1 CGGGCAATTA CCTCACTAAA GGGAACAAAG CTGGGTACCG GATCCTAGTG AACCTCTTCG
             61 AGGGACCTAA TAACTTCGTA TAGCATACAT TATACGAAGT TATATTAAGG GTTCCGGATC
            121 TCGACCAGCT TCTGATGGAA TTAGAACTTG GCAAAACAAT ACTGAGAATG AAGTGTATGT
            181 GGAACAGAGG CTGCTGATCT CGTTCTTCAG GCTATGAAAC TGACACATTT GGAAACCACA
            241 GTACTTAGAA CCACAAAGTG GGAATCAAGA GAAAAACAAT GATCCCACGA GAGATCTATA
            301 GATCTATAGA TCATGAGTGG GAGGAATGAG CTGGCCCTTA ATTTGGTTTT GCTTGTTTAA
            361 ATTATGATAT CCAACTATGA AACATTATCA TAAAGCAATA GTAAAGAGCC TTCAGTAAAG
            421 AGCAGGCATT TATCTAATCC CACCCCACCC CCACCCCCGT AGCTCCAATC CTTCCATTCA
            481 AAATGTAGGT ACTCTGTTCT CACCCTTCTT AACAAAGTAT GACAGGAAAA ACTTCCATTT
            541 TAGTGGACAT CTTTATTGTT TAATAGATCA TCAATTTCTG CATCCCGGGG ATCTGATATC
            601 ATCGATTAGC CCTCCCACAC ATAACCAGAG GGCAGCAATT CACGAATCCC AACTGCCGTC
            661 GGCTGTCCAT CACTGTCCTT CACTATGGCT TTGATCCCAG GATGCAGATC GAGAAGCACC
            721 TGTCGGCACC GTCCGCAGGG GCTCAAGATG CCCCTGTTCT CATTTCCGAT CGCGACGATA
            781 CAAGTCAGGT TGCCAGCTGC CGCAGCAGCA GCAGTGCCCA GCACCACGAG TTCTGCACAA
            841 GGTCCCCCCA GTAAAATGAT ATACATTGAC ACCAGTGAAG ATGCGGCCGT CGCTAGAGAG
            901 AGCTGCGCTG GCGACGCTGT AGTCTTCAGA GATGGGGATG CTGTTGATTG TAGCCGTTGC
            961 TCTTTCAATG AGGGTGGGAT TCTTCTTGAG ACAAAGGCTT GGACCAATGG GTGGGCTCGA
           1021 GATCCTAGAT GGATGCAGGT CGAAAGGCCC CGAGATGAGG AAGAGAAGAA CCAGCGCCGG
          1081 TCCAGAACCG GATGC
          //
          Primer: PGK_N_rev
          Region: insert mid (loxP-1, SV40 pA, mClover)
          Sequence file: RDB16820_A8Lpc.seq check
          >D06157B7_A8Lp_1_PGK_N_rev_C07_07_ABI24.ab1
              1 CGTATGGGCC GCCTCCCTAC CCGGTAGATT TCGAGGTCGA GATCCTAAGC TTGGCTGGAC
             61 GTAAACTCCT CTTCAGACCT AATAACTTCG TATAGCATAC ATTATACGAA GTTATATTAA
            121 GGGTTATTGA ATATGATCGA CGCGTTAAGA TACATTGATG AGTTTGGACA AACCACAACT
            181 AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA
            241 ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG
            301 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG
            361 GCTGATTATG ATCAGTTATC TAGATTACTT GTACAGCTCG TCCATGCCAT GTGTAATCCC
            421 GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT
            481 CAGCTTGGAC TGATGGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT
            541 GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCAA CGTTGTGGCG
            601 GATCTTGAAG TTAGCCTTGA TGCCGTTCTT CTGCTTGTCG GCCGTGATAT AGACGTTGTG
            661 GCTGTTGAAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT
            721 GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT
            781 CTTGTAGGTA CCGTCGTCCT TGAAAGAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT
            841 GGCGGACTTG AAAGAAGTCG TGCTGCTTCA TGTGGTCTGG GTAGCGGCTG AAGCAGCCAC
            901 GCCGTAGCCG AAATGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GGTGGTGCAG
            961 ATGAACTTCA GGGTCAGCTT GCCGTTGGTG GCATCGCCCT CGCCCTCGCC GCGACGCTGG
           1021 AACTTGTGAC GGTTACGTCG CCGGTCCAGC TCGACCAGAA TGGCACCACC CTGTGGAAAC
          1081 AGCTCCT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

          2021.11.02

          GNP_filter3_RDBDEP_html_211029.pl