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pMK388 (mClover-BSD)

A plasmid for construction of C-terminal mClover-BSD tagging donors.

Catalog number RDB16816
Resource name pMK388 (mClover-BSD)
Clone info. A plasmid for construction of C-terminal mClover-BSD tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Aequorea victoria GFP cDNA
Depositor Kanemaki, Masato |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16816 pMK388 (mClover-BSD) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16816_A8Lpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 RDB16816_A8Lpa.seq
>D06157B3_A8LP_2_pUC-M13_-100_A06_03_ABI24.ab1
    1 GGTCGGTAAG CGAATTAGTT GGGTACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG
   61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CGGATCCGGT
  121 GCAGGCGCCG CTAGCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC
  181 CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTCCGCGG CGAGGGCGAG
  241 GGCGATGCCA CCAACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC
  301 GTGCCCTGGC CCACCCTCGT GACCACCTTC GGCTACGGCG TGGCCTGCTT CAGCCGCTAC
  361 CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG
  421 GAGCGCACCA TCTCTTTCAA GGACGACGGT ACCTACAAGA CCCGCGCCGA GGTGAAGTTC
  481 GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC
  541 AACATCCTGG GGCACAAGCT GGAGTACAAC TTCAACAGCC ACAACGTCTA TATCACGGCC
  601 GACAAGCAGA AGAACGGCAT CAAGGCTAAC TTCAAGATCC GCCACAACGT TGAGGACGGC
  661 AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG
  721 CTGCCCGACA ACCACTACCT GAGCCATCAG TCCAAGCTGA GCAAAGACCC CAACGAGAAG
  781 CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATTACACA TGGCATGGAC
  841 GAGCTGTACA AGTAATCTAG ATAACTGATC ATAATCAGCC ATACCACATT TGTAGAGGTT
  901 TTACTTGCTT TAAAAAAACC TCCCACACCT CCCCCTGAAA CCTGAAACAT AAAAATGAAT
  961 GCAATTTGTT GTTGTTTAAC TTTGTTTTAA TTGCAGCTTT ATAATGGTTA CAATAAGCCA
 1021 TAGCCATCAC AAATTCACAA TTAAGCCATT TTTTTTCACT GCACTCTAGG TGGTGGCTTG
1081 ATCCGAGCTC CAAC
//
Primer: reverse RDB16816_A8Lpb.seq
>D06157B3_A8LP_2_reverse_B06_06_ABI24.ab1
    1 CTGACCATTT CCCCTCACTA AGGGACAAAA GCTGGGTACC GGATCCTAGT GAACCTCTTC
   61 GAGGGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG GGTTCCGGAT
  121 CTCGACCAGC TTCTGATGGA ATTAGAACTT GGCAAAACAA TACTGAGAAT GAAGTGTATG
  181 TGGAACAGAG GCTGCTGATC TCGTTCTTCA GGCTATGAAA CTGACACATT TGGAAACCAC
  241 AGTACTTAGA ACCACAAAGT GGGAATCAAG AGAAAAACAA TGATCCCACG AGAGATCTAT
  301 AGATCTATAG ATCATGAGTG GGAGGAATGA GCTGGCCCTT AATTTGGTTT TGCTTGTTTA
  361 AATTATGATA TCCAACTATG AAACATTATC ATAAAGCAAT AGTAAAGAGC CTTCAGTAAA
  421 GAGCAGGCAT TTATCTAATC CCACCCCACC CCCACCCCCG TAGCTCCAAT CCTTCCATTC
  481 AAAATGTAGG TACTCTGTTC TCACCCTTCT TAACAAAGTA TGACAGGAAA AACTTCCATT
  541 TTAGTGGACA TCTTTATTGT TTAATAGATC ATCAATTTCT GCATCCCGGG GATCTGATAT
  601 CATCGATTAG CCCTCCCACA CATAACCAGA GGGCAGCAAT TCACGAATCC CAACTGCCGT
  661 CGGCTGTCCA TCACTGTCCT TCACTATGGC TTTGATCCCA GGATGCAGAT CGAGAAGCAC
  721 CTGTCGGCAC CGTCCGCAGG GGCTCAAGAT GCCCCTGTTC TCATTTCCGA TCGCGACGAT
  781 ACAAGTCAGG TTGCCAGCTG CCGCAGCAGC AGCAGTGCCC AGCACCACGA GTTCTGCACA
  841 AGGTCCCCCC AGTAAAATGA TATACATTGA CACCAGTGAA GATGCGGCCG TCGCTAGAGA
  901 GAGCTGCGCT GGCGACGCTG TAGTCTTCAG AGATGGGGGA TGCTGTTGAT TGTAGCCCGT
  961 TGCTCTTTCA ATGAGGGTGG GATTCTTTCT TGAGACAAAG GCTTGATCAT GATGGGCTCG
 1021 GAGATCTAGA TGAATGCAAG TCGAAGGTCC GAGATGAGGA GAGAAGTACC AGCGCCGCAG
1081 GACCGATGCT G
//
Primer: PGK_N_rev RDB16816_A8Lpc.seq
>D06157B3_A8LP_2_PGK_N_rev_C06_09_ABI24.ab1
    1 CCAGGGGGCG GCTCCCTACC CGGTAGATTT CGAGGTCGAG ATCCTAAGCT TGGCTGGACG
   61 TAAACTCCTC TTCAGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG
  121 GGTTATTGAA TATGATCGAC GCGTTAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA
  181 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA
  241 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG
  301 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
  361 CTGATTATGA TCAGTTATCT AGATTACTTG TACAGCTCGT CCATGCCATG TGTAATCCCG
  421 GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC
  481 AGCTTGGACT GATGGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG
  541 GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCTCAAC GTTGTGGCGG
  601 ATCTTGAAGT TAGCCTTGAT GCCGTTCTTC TGCTTGTCGG CCGTGATATA GACGTTGTGG
  661 CTGTTGAAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG
  721 CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC
  781 TTGTAGGTAC CGTCGTCCTT GAAAGAGATG GTGCGCTCCT GGACGTAGCC TTCGGGCATG
  841 GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGCTGAA GCAGGCCACG
  901 CCGTAGCCGA AGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GGTGGGTGCA
  961 GATGAACTTC AGGGTCAGCT TGCCGTTGGG TGGGCATCGC CCTCGCCCTC GCCGCGGACG
 1021 CTGACTTGTG GCCGTTTAAC GTTCGGCGTC CAGCTCGAAC CAGGATGGCA TACCTTCGGT
1081 GAACCAGCTC CCTCGACCCC CATG
//
Primer: PGKpro_F RDB16816_A8Lpd.seq
>D06157B3_A8LP_2_PGKpro_F_D06_12_ABI24.ab1
    1 ACCCCCGCCG TCTCTCTCTT CTCATCTCCG GGCCTTTCGA CCTGCATCCA TCTAGATCTC
   61 GAGCCACCAT GGCCAAGCCT TTGTCTCAAG AAGAATCCAC CCTCATTGAA AGAGCAACGG
  121 CTACAATCAA CAGCATCCCC ATCTCTGAAG ACTACAGCGT CGCCAGCGCA GCTCTCTCTA
  181 GCGACGGCCG CATCTTCACT GGTGTCAATG TATATCATTT TACTGGGGGA CCTTGTGCAG
  241 AACTCGTGGT GCTGGGCACT GCTGCTGCTG CGGCAGCTGG CAACCTGACT TGTATCGTCG
  301 CGATCGGAAA TGAGAACAGG GGCATCTTGA GCCCCTGCGG ACGGTGCCGA CAGGTGCTTC
  361 TCGATCTGCA TCCTGGGATC AAAGCCATAG TGAAGGACAG TGATGGACAG CCGACGGCAG
  421 TTGGGATTCG TGAATTGCTG CCCTCTGGTT ATGTGTGGGA GGGCTAATCG ATGATATCAG
  481 ATCCCCGGGA TGCAGAAATT GATGATCTAT TAAACAATAA AGATGTCCAC TAAAATGGAA
  541 GTTTTTCCTG TCATACTTTG TTAAGAAGGG TGAGAACAGA GTACCTACAT TTTGAATGGA
  601 AGGATTGGAG CTACGGGGGT GGGGGTGGGG TGGGATTAGA TAAATGCCTG CTCTTTACTG
  661 AAGGCTCTTT ACTATTGCTT TATGATAATG TTTCATAGTT GGATATCATA ATTTAAACAA
  721 GCAAAACCAA ATTAAGGGCC AGCTCATTCC TCCCACTCAT GATCTATAGA TCTATAGATC
  781 TCTCGTGGGA TCATTGTTTT TCTCTTGATT CCCACTTTGT GGTTCTAAGT ACTGTGGTTT
  841 CCAAATGTGT CAGTTTCATA GCCTGAAGAA CGAGATCAGC AGCCTCTGTT CCACATACAC
  901 TTCATTCTCA GTATTGTTTT TGCCAAGTTC TAATTCCATC AGAAGCTGGG TCGAGATCCC
  961 GGAAACCCTT AATATAACTT CGTATAATGT ATGCTATACG AAAGTTATTA GGTCCCTCGA
 1021 GAGGTTTCAC TAGGATCCGG GTACCCAAGC CTTTTGATCC CCATTAGTGA GGATATGGCG
1081 CGGCTGGCGG TAAATTCAAT TTGGATCA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

2019.07.12

GNP_filter3_RDBDEP_html_190431.pl