Sequencing and PCR primers for primer information.
A plasmid for construction of C-terminal mClover-BSD tagging donors.
| Catalog number | RDB16816 |
|---|---|
| Resource name | pMK388 (mClover-BSD) |
| Clone info. | A plasmid for construction of C-terminal mClover-BSD tagging donors. |
| Vector backbone | pBluescript (plasmid) |
| Size of vector backbone | 2.9 kb |
| Selectable markers | Am^r (E. coli) |
| Gene/insert name | Aequorea victoria GFP cDNA |
| Depositor | Kanemaki, Masato | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB16816 | pMK388 (mClover-BSD) | DNA solution |
Electronic file
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Auxin Inducible Degron (AID) System (English text) |
|---|---|
| Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB16816_A8Lpp1.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
| Primer: pUC-M13_-100 | RDB16816_A8Lpa.seq |
|---|---|
|
>D06157B3_A8LP_2_pUC-M13_-100_A06_03_ABI24.ab1 1 GGTCGGTAAG CGAATTAGTT GGGTACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG 61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CGGATCCGGT 121 GCAGGCGCCG CTAGCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC 181 CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTCCGCGG CGAGGGCGAG 241 GGCGATGCCA CCAACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC 301 GTGCCCTGGC CCACCCTCGT GACCACCTTC GGCTACGGCG TGGCCTGCTT CAGCCGCTAC 361 CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG 421 GAGCGCACCA TCTCTTTCAA GGACGACGGT ACCTACAAGA CCCGCGCCGA GGTGAAGTTC 481 GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC 541 AACATCCTGG GGCACAAGCT GGAGTACAAC TTCAACAGCC ACAACGTCTA TATCACGGCC 601 GACAAGCAGA AGAACGGCAT CAAGGCTAAC TTCAAGATCC GCCACAACGT TGAGGACGGC 661 AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG 721 CTGCCCGACA ACCACTACCT GAGCCATCAG TCCAAGCTGA GCAAAGACCC CAACGAGAAG 781 CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATTACACA TGGCATGGAC 841 GAGCTGTACA AGTAATCTAG ATAACTGATC ATAATCAGCC ATACCACATT TGTAGAGGTT 901 TTACTTGCTT TAAAAAAACC TCCCACACCT CCCCCTGAAA CCTGAAACAT AAAAATGAAT 961 GCAATTTGTT GTTGTTTAAC TTTGTTTTAA TTGCAGCTTT ATAATGGTTA CAATAAGCCA 1021 TAGCCATCAC AAATTCACAA TTAAGCCATT TTTTTTCACT GCACTCTAGG TGGTGGCTTG 1081 ATCCGAGCTC CAAC // |
|
| Primer: reverse | RDB16816_A8Lpb.seq |
|
>D06157B3_A8LP_2_reverse_B06_06_ABI24.ab1 1 CTGACCATTT CCCCTCACTA AGGGACAAAA GCTGGGTACC GGATCCTAGT GAACCTCTTC 61 GAGGGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG GGTTCCGGAT 121 CTCGACCAGC TTCTGATGGA ATTAGAACTT GGCAAAACAA TACTGAGAAT GAAGTGTATG 181 TGGAACAGAG GCTGCTGATC TCGTTCTTCA GGCTATGAAA CTGACACATT TGGAAACCAC 241 AGTACTTAGA ACCACAAAGT GGGAATCAAG AGAAAAACAA TGATCCCACG AGAGATCTAT 301 AGATCTATAG ATCATGAGTG GGAGGAATGA GCTGGCCCTT AATTTGGTTT TGCTTGTTTA 361 AATTATGATA TCCAACTATG AAACATTATC ATAAAGCAAT AGTAAAGAGC CTTCAGTAAA 421 GAGCAGGCAT TTATCTAATC CCACCCCACC CCCACCCCCG TAGCTCCAAT CCTTCCATTC 481 AAAATGTAGG TACTCTGTTC TCACCCTTCT TAACAAAGTA TGACAGGAAA AACTTCCATT 541 TTAGTGGACA TCTTTATTGT TTAATAGATC ATCAATTTCT GCATCCCGGG GATCTGATAT 601 CATCGATTAG CCCTCCCACA CATAACCAGA GGGCAGCAAT TCACGAATCC CAACTGCCGT 661 CGGCTGTCCA TCACTGTCCT TCACTATGGC TTTGATCCCA GGATGCAGAT CGAGAAGCAC 721 CTGTCGGCAC CGTCCGCAGG GGCTCAAGAT GCCCCTGTTC TCATTTCCGA TCGCGACGAT 781 ACAAGTCAGG TTGCCAGCTG CCGCAGCAGC AGCAGTGCCC AGCACCACGA GTTCTGCACA 841 AGGTCCCCCC AGTAAAATGA TATACATTGA CACCAGTGAA GATGCGGCCG TCGCTAGAGA 901 GAGCTGCGCT GGCGACGCTG TAGTCTTCAG AGATGGGGGA TGCTGTTGAT TGTAGCCCGT 961 TGCTCTTTCA ATGAGGGTGG GATTCTTTCT TGAGACAAAG GCTTGATCAT GATGGGCTCG 1021 GAGATCTAGA TGAATGCAAG TCGAAGGTCC GAGATGAGGA GAGAAGTACC AGCGCCGCAG 1081 GACCGATGCT G // |
|
| Primer: PGK_N_rev | RDB16816_A8Lpc.seq |
|
>D06157B3_A8LP_2_PGK_N_rev_C06_09_ABI24.ab1 1 CCAGGGGGCG GCTCCCTACC CGGTAGATTT CGAGGTCGAG ATCCTAAGCT TGGCTGGACG 61 TAAACTCCTC TTCAGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG 121 GGTTATTGAA TATGATCGAC GCGTTAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA 181 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA 241 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG 301 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG 361 CTGATTATGA TCAGTTATCT AGATTACTTG TACAGCTCGT CCATGCCATG TGTAATCCCG 421 GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC 481 AGCTTGGACT GATGGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG 541 GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCTCAAC GTTGTGGCGG 601 ATCTTGAAGT TAGCCTTGAT GCCGTTCTTC TGCTTGTCGG CCGTGATATA GACGTTGTGG 661 CTGTTGAAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG 721 CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC 781 TTGTAGGTAC CGTCGTCCTT GAAAGAGATG GTGCGCTCCT GGACGTAGCC TTCGGGCATG 841 GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGCTGAA GCAGGCCACG 901 CCGTAGCCGA AGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GGTGGGTGCA 961 GATGAACTTC AGGGTCAGCT TGCCGTTGGG TGGGCATCGC CCTCGCCCTC GCCGCGGACG 1021 CTGACTTGTG GCCGTTTAAC GTTCGGCGTC CAGCTCGAAC CAGGATGGCA TACCTTCGGT 1081 GAACCAGCTC CCTCGACCCC CATG // |
|
| Primer: PGKpro_F | RDB16816_A8Lpd.seq |
|
>D06157B3_A8LP_2_PGKpro_F_D06_12_ABI24.ab1 1 ACCCCCGCCG TCTCTCTCTT CTCATCTCCG GGCCTTTCGA CCTGCATCCA TCTAGATCTC 61 GAGCCACCAT GGCCAAGCCT TTGTCTCAAG AAGAATCCAC CCTCATTGAA AGAGCAACGG 121 CTACAATCAA CAGCATCCCC ATCTCTGAAG ACTACAGCGT CGCCAGCGCA GCTCTCTCTA 181 GCGACGGCCG CATCTTCACT GGTGTCAATG TATATCATTT TACTGGGGGA CCTTGTGCAG 241 AACTCGTGGT GCTGGGCACT GCTGCTGCTG CGGCAGCTGG CAACCTGACT TGTATCGTCG 301 CGATCGGAAA TGAGAACAGG GGCATCTTGA GCCCCTGCGG ACGGTGCCGA CAGGTGCTTC 361 TCGATCTGCA TCCTGGGATC AAAGCCATAG TGAAGGACAG TGATGGACAG CCGACGGCAG 421 TTGGGATTCG TGAATTGCTG CCCTCTGGTT ATGTGTGGGA GGGCTAATCG ATGATATCAG 481 ATCCCCGGGA TGCAGAAATT GATGATCTAT TAAACAATAA AGATGTCCAC TAAAATGGAA 541 GTTTTTCCTG TCATACTTTG TTAAGAAGGG TGAGAACAGA GTACCTACAT TTTGAATGGA 601 AGGATTGGAG CTACGGGGGT GGGGGTGGGG TGGGATTAGA TAAATGCCTG CTCTTTACTG 661 AAGGCTCTTT ACTATTGCTT TATGATAATG TTTCATAGTT GGATATCATA ATTTAAACAA 721 GCAAAACCAA ATTAAGGGCC AGCTCATTCC TCCCACTCAT GATCTATAGA TCTATAGATC 781 TCTCGTGGGA TCATTGTTTT TCTCTTGATT CCCACTTTGT GGTTCTAAGT ACTGTGGTTT 841 CCAAATGTGT CAGTTTCATA GCCTGAAGAA CGAGATCAGC AGCCTCTGTT CCACATACAC 901 TTCATTCTCA GTATTGTTTT TGCCAAGTTC TAATTCCATC AGAAGCTGGG TCGAGATCCC 961 GGAAACCCTT AATATAACTT CGTATAATGT ATGCTATACG AAAGTTATTA GGTCCCTCGA 1021 GAGGTTTCAC TAGGATCCGG GTACCCAAGC CTTTTGATCC CCATTAGTGA GGATATGGCG 1081 CGGCTGGCGG TAAATTCAAT TTGGATCA // |
|
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
2019.07.12
GNP_filter3_RDBDEP_html_190431.pl