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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK388 (mClover-BSD)

A plasmid for construction of C-terminal mClover-BSD tagging donors.

Catalog number RDB16816
Resource name pMK388 (mClover-BSD)
Clone info. A plasmid for construction of C-terminal mClover-BSD tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Kanemaki, Masato |
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External Database
Aequorea victoria GFP

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
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            提供同意書 (MTA, 営利目的用)[Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。

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            RDB16816 pMK388 (mClover-BSD) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pMK388 (mClover-BSD) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16816).

            Reference section:

            Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


            Tips

            Featured content

            Featured content Auxin Inducible Degron (AID) System (English text)
            Featured content Auxin Inducible Degron (AID) System (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB16816_A8Lpp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pUC-M13_-100 (Pr0310)
            Region: T7 pro,insert 5'(Linker,mClover 5')
            Sequence file: RDB16816_A8Lpe.seq check
            >D06157B3_A8LP_2_pUC-M13_-100_A06_03_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NGGNANGCNN GGNNTTTCCC NGTCNCGACG TTGTAAAACG
               61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CGGATCCGGT
              121 GCAGGCGCCG CTAGCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC
              181 CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTCCGCGG CGAGGGCGAG
              241 GGCGATGCCA CCAACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC
              301 GTGCCCTGGC CCACCCTCGT GACCACCTTC GGCTACGGCG TGGCCTGCTT CAGCCGCTAC
              361 CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG
              421 GAGCGCACCA TCTCTTTCAA GGACGACGGT ACCTACAAGA CCCGCGCCGA GGTGAAGTTC
              481 GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC
              541 AACATCCTGG GGCACAAGCT GGAGTACAAC TTCAACAGCC ACAACGTCTA TATCACGGCC
              601 GACAAGCAGA AGAACGGCAT CAAGGCTAAC TTCAAGATCC GCCACAACGT TGAGGACGGC
              661 AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACNG CCCCGTGCTG
              721 CTGCCCGACA ACCACTACCT GANCCATCAG TCCAAGCTGA NCAAAGANCC CAACGANAAN
              781 CGCGATCACA TGGNCCTGCT GGNNTTCGTG ANCGCCGCCG GGANTACACA TGGNATGGNN
              841 GANCTGTNCN AGTAATCTAG ATANNTGATC ATAATCAGCC NTACCACATT TGNANNNGNT
              901 TNNNTNGCTT NAAAAAAANC NNCCNCNNNN NCCCNNNNAN NNNNNANNNN NANNNNNNNN
            961 GNNNNNNNNN N
            //
            Primer: reverse (Pr0099)
            Region: insert 3'(mPGK ter,BSD)
            Sequence file: RDB16816_A8Lpf.seq check
            >D06157B3_A8LP_2_reverse_B06_06_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN ANGNNNNNAA GCTGGGTANC GGNTCCTAGT GAACCTCTTC
               61 GAGGGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG GGTTCCGGAT
              121 CTCGACCAGC TTCTGATGGA ATTAGAACTT GGCAAAACAA TACTGAGAAT GAAGTGTATG
              181 TGGAACAGAG GCTGCTGATC TCGTTCTTCA GGCTATGAAA CTGACACATT TGGAAACCAC
              241 AGTACTTAGA ACCACAAAGT GGGAATCAAG AGAAAAACAA TGATCCCACG AGAGATCTAT
              301 AGATCTATAG ATCATGAGTG GGAGGAATGA GCTGGCCCTT AATTTGGTTT TGCTTGTTTA
              361 AATTATGATA TCCAACTATG AAACATTATC ATAAAGCAAT AGTAAAGAGC CTTCAGTAAA
              421 GAGCAGGCAT TTATCTAATC CCACCCCACC CCCACCCCCG TAGCTCCAAT CCTTCCATTC
              481 AAAATGTAGG TACTCTGTTC TCACCCTTCT TAACAAAGTA TGACAGGAAA AACTTCCATT
              541 TTAGTGGACA TCTTTATTGT TTAATAGATC ATCAATTTCT GCATCCCGGG GATCTGATAT
              601 CATCGATTAG CCCTCCCACA CATAACCAGA GGGCAGCAAT TCACGAATCC CAACTGCCGT
              661 CGGCTGTCCA TCACTGTCCT TCACTATGGC TTTGATCCCA GGATGCAGAT CGAGAAGCAC
              721 CTGTCGGCAC CGTCCGCAGG GGCTCAAGAT GCCCCTGTTC TCATTTCCGA TCGCGACGAT
              781 ACAAGTCANG TTGCCNGCTG CCGCANCAGC ANCAGTGCCC AGCACCACGA GTTCTGCACA
              841 AGGTCCCCCC AGNNAAATGA TATACATTGA CACCNGNGAA GATGCGGNNG TCNCNNNANA
            901 NANCNNCGNN NGNNANNCNN NANNCNNCNN NNATGGGNNA NGNNNNNNNN NN
            //
            Primer: PGK_N_rev (Pr0243)
            Region: insert mid(loxP,SV40 pA,mClover 3')
            Sequence file: RDB16816_A8Lpg.seq check
            >D06157B3_A8LP_2_PGK_N_rev_C06_09_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNC NGGNANANTT CGAGGTCGAG NTCCTAAGCT TGGCTGGACG
               61 TAAACTCCTC TTCAGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG
              121 GGTTATTGAA TATGATCGAC GCGTTAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA
              181 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA
              241 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG
              301 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
              361 CTGATTATGA TCAGTTATCT AGATTACTTG TACAGCTCGT CCATGCCATG TGTAATCCCG
              421 GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC
              481 AGCTTGGACT GATGGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG
              541 GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCTCAAC GTTGTGGCGG
              601 ATCTTGAAGT TAGCCTTGAT GCCGTTCTTC TGCTTGTCGG CCGTGATATA GACGTTGTGG
              661 CTGTTGAAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG
              721 CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC
              781 TTGTANGTAC CGTCGTCCTT NNNAGAGATG GTGCGCTCCT GGACGTANCC TTCGGGCATG
              841 GCGGANTTGA AGAANTCGNG CTGCTTCATG TGGNCNGGGN ANCGGCNGNN NCANGCCACG
              901 CCNNANCCNA NNNNGGNCAC NANGGNNGGN CAGGNNNNGG GCNNCTNNNN NNNNGNNNNN
            961 NNNNN
            //
            Primer: PGKpro_F (Pr0249)
            Region: insert mid(mPGK ter,BSD)
            Sequence file: RDB16816_A8Lph.seq check
            >D06157B3_A8LP_2_PGKpro_F_D06_12_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNTN NNNNTCTCCG GGCCTTTCGA NCTGCATCCN TCTAGATCTC
               61 GAGCCACCAT GGCCNAGCCT TTGTCTCAAG AAGAATCCAC CCTCATTGAA AGAGCAACGG
              121 CTACAATCAA CAGCATCCCC ATCTCTGAAG ACTACAGCGT CGCCAGCGCA GCTCTCTCTA
              181 GCGACGGCCG CATCTTCACT GGTGTCAATG TATATCATTT TACTGGGGGA CCTTGTGCAG
              241 AACTCGTGGT GCTGGGCACT GCTGCTGCTG CGGCAGCTGG CAACCTGACT TGTATCGTCG
              301 CGATCGGAAA TGAGAACAGG GGCATCTTGA GCCCCTGCGG ACGGTGCCGA CAGGTGCTTC
              361 TCGATCTGCA TCCTGGGATC AAAGCCATAG TGAAGGACAG TGATGGACAG CCGACGGCAG
              421 TTGGGATTCG TGAATTGCTG CCCTCTGGTT ATGTGTGGGA GGGCTAATCG ATGATATCAG
              481 ATCCCCGGGA TGCAGAAATT GATGATCTAT TAAACAATAA AGATGTCCAC TAAAATGGAA
              541 GTTTTTCCTG TCATACTTTG TTAAGAAGGG TGAGAACAGA GTACCTACAT TTTGAATGGA
              601 AGGATTGGAG CTACGGGGGT GGGGGTGGGG TGGGATTAGA TAAATGCCTG CTCTTTACTG
              661 AAGGCTCTTT ACTATTGCTT TATGATAATG TTTCATAGTT GGATATCATA ATTTAAACAA
              721 GCAAAACCAA ATTAAGGGNC AGCTCATTCC TCCCACTCAT GATCTATAGA TCTATAGATC
              781 TCTCGTGNGA TCATTGTTTT TCTCTTGATT CNNACTTTGT GGTTCTAAGT ACTGTGGTTT
              841 CCAAATGTGT CAGTTTCATA GCCNGAANAA CGAGATCAGC ANCCTCNGTT CCNCNTACNC
              901 TTCNTTCTCA GTATTGNTTT TGNNNNNNNC NAANNNCNTC ANAAGCNNGN NCGANATNNN
            961 NNNANNNNTN NNNNNNNNTT NNNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

            2023.10.05

            GNP_filter3_RDBDEP_html_231001.pl