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pMK388 (mClover-BSD) (#RDB16816)

A plasmid for construction of C-terminal mClover-BSD tagging donors.

Clone info. A plasmid for construction of C-terminal mClover-BSD tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Kanemaki, Masato |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。

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RDB16816 pMK388 (mClover-BSD) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMK388 (mClover-BSD) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16816).

Reference section:

Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16816_A8Lpp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310)
Region: T7 pro,insert 5'(Linker,mClover 5')
Sequence file: RDB16816_A8Lpe.seq check
>D06157B3_A8LP_2_pUC-M13_-100_A06_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGGNANGCNN GGNNTTTCCC NGTCNCGACG TTGTAAAACG
   61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CGGATCCGGT
  121 GCAGGCGCCG CTAGCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC
  181 CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTCCGCGG CGAGGGCGAG
  241 GGCGATGCCA CCAACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC
  301 GTGCCCTGGC CCACCCTCGT GACCACCTTC GGCTACGGCG TGGCCTGCTT CAGCCGCTAC
  361 CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG
  421 GAGCGCACCA TCTCTTTCAA GGACGACGGT ACCTACAAGA CCCGCGCCGA GGTGAAGTTC
  481 GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC
  541 AACATCCTGG GGCACAAGCT GGAGTACAAC TTCAACAGCC ACAACGTCTA TATCACGGCC
  601 GACAAGCAGA AGAACGGCAT CAAGGCTAAC TTCAAGATCC GCCACAACGT TGAGGACGGC
  661 AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACNG CCCCGTGCTG
  721 CTGCCCGACA ACCACTACCT GANCCATCAG TCCAAGCTGA NCAAAGANCC CAACGANAAN
  781 CGCGATCACA TGGNCCTGCT GGNNTTCGTG ANCGCCGCCG GGANTACACA TGGNATGGNN
  841 GANCTGTNCN AGTAATCTAG ATANNTGATC ATAATCAGCC NTACCACATT TGNANNNGNT
  901 TNNNTNGCTT NAAAAAAANC NNCCNCNNNN NCCCNNNNAN NNNNNANNNN NANNNNNNNN
961 GNNNNNNNNN N
//
Primer: reverse (Pr0099)
Region: insert 3'(mPGK ter,BSD)
Sequence file: RDB16816_A8Lpf.seq check
>D06157B3_A8LP_2_reverse_B06_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN ANGNNNNNAA GCTGGGTANC GGNTCCTAGT GAACCTCTTC
   61 GAGGGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG GGTTCCGGAT
  121 CTCGACCAGC TTCTGATGGA ATTAGAACTT GGCAAAACAA TACTGAGAAT GAAGTGTATG
  181 TGGAACAGAG GCTGCTGATC TCGTTCTTCA GGCTATGAAA CTGACACATT TGGAAACCAC
  241 AGTACTTAGA ACCACAAAGT GGGAATCAAG AGAAAAACAA TGATCCCACG AGAGATCTAT
  301 AGATCTATAG ATCATGAGTG GGAGGAATGA GCTGGCCCTT AATTTGGTTT TGCTTGTTTA
  361 AATTATGATA TCCAACTATG AAACATTATC ATAAAGCAAT AGTAAAGAGC CTTCAGTAAA
  421 GAGCAGGCAT TTATCTAATC CCACCCCACC CCCACCCCCG TAGCTCCAAT CCTTCCATTC
  481 AAAATGTAGG TACTCTGTTC TCACCCTTCT TAACAAAGTA TGACAGGAAA AACTTCCATT
  541 TTAGTGGACA TCTTTATTGT TTAATAGATC ATCAATTTCT GCATCCCGGG GATCTGATAT
  601 CATCGATTAG CCCTCCCACA CATAACCAGA GGGCAGCAAT TCACGAATCC CAACTGCCGT
  661 CGGCTGTCCA TCACTGTCCT TCACTATGGC TTTGATCCCA GGATGCAGAT CGAGAAGCAC
  721 CTGTCGGCAC CGTCCGCAGG GGCTCAAGAT GCCCCTGTTC TCATTTCCGA TCGCGACGAT
  781 ACAAGTCANG TTGCCNGCTG CCGCANCAGC ANCAGTGCCC AGCACCACGA GTTCTGCACA
  841 AGGTCCCCCC AGNNAAATGA TATACATTGA CACCNGNGAA GATGCGGNNG TCNCNNNANA
901 NANCNNCGNN NGNNANNCNN NANNCNNCNN NNATGGGNNA NGNNNNNNNN NN
//
Primer: PGK_N_rev (Pr0243)
Region: insert mid(loxP,SV40 pA,mClover 3')
Sequence file: RDB16816_A8Lpg.seq check
>D06157B3_A8LP_2_PGK_N_rev_C06_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNC NGGNANANTT CGAGGTCGAG NTCCTAAGCT TGGCTGGACG
   61 TAAACTCCTC TTCAGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG
  121 GGTTATTGAA TATGATCGAC GCGTTAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA
  181 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA
  241 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG
  301 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
  361 CTGATTATGA TCAGTTATCT AGATTACTTG TACAGCTCGT CCATGCCATG TGTAATCCCG
  421 GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC
  481 AGCTTGGACT GATGGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG
  541 GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCTCAAC GTTGTGGCGG
  601 ATCTTGAAGT TAGCCTTGAT GCCGTTCTTC TGCTTGTCGG CCGTGATATA GACGTTGTGG
  661 CTGTTGAAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG
  721 CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC
  781 TTGTANGTAC CGTCGTCCTT NNNAGAGATG GTGCGCTCCT GGACGTANCC TTCGGGCATG
  841 GCGGANTTGA AGAANTCGNG CTGCTTCATG TGGNCNGGGN ANCGGCNGNN NCANGCCACG
  901 CCNNANCCNA NNNNGGNCAC NANGGNNGGN CAGGNNNNGG GCNNCTNNNN NNNNGNNNNN
961 NNNNN
//
Primer: PGKpro_F (Pr0249)
Region: insert mid(mPGK ter,BSD)
Sequence file: RDB16816_A8Lph.seq check
>D06157B3_A8LP_2_PGKpro_F_D06_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNTN NNNNTCTCCG GGCCTTTCGA NCTGCATCCN TCTAGATCTC
   61 GAGCCACCAT GGCCNAGCCT TTGTCTCAAG AAGAATCCAC CCTCATTGAA AGAGCAACGG
  121 CTACAATCAA CAGCATCCCC ATCTCTGAAG ACTACAGCGT CGCCAGCGCA GCTCTCTCTA
  181 GCGACGGCCG CATCTTCACT GGTGTCAATG TATATCATTT TACTGGGGGA CCTTGTGCAG
  241 AACTCGTGGT GCTGGGCACT GCTGCTGCTG CGGCAGCTGG CAACCTGACT TGTATCGTCG
  301 CGATCGGAAA TGAGAACAGG GGCATCTTGA GCCCCTGCGG ACGGTGCCGA CAGGTGCTTC
  361 TCGATCTGCA TCCTGGGATC AAAGCCATAG TGAAGGACAG TGATGGACAG CCGACGGCAG
  421 TTGGGATTCG TGAATTGCTG CCCTCTGGTT ATGTGTGGGA GGGCTAATCG ATGATATCAG
  481 ATCCCCGGGA TGCAGAAATT GATGATCTAT TAAACAATAA AGATGTCCAC TAAAATGGAA
  541 GTTTTTCCTG TCATACTTTG TTAAGAAGGG TGAGAACAGA GTACCTACAT TTTGAATGGA
  601 AGGATTGGAG CTACGGGGGT GGGGGTGGGG TGGGATTAGA TAAATGCCTG CTCTTTACTG
  661 AAGGCTCTTT ACTATTGCTT TATGATAATG TTTCATAGTT GGATATCATA ATTTAAACAA
  721 GCAAAACCAA ATTAAGGGNC AGCTCATTCC TCCCACTCAT GATCTATAGA TCTATAGATC
  781 TCTCGTGNGA TCATTGTTTT TCTCTTGATT CNNACTTTGT GGTTCTAAGT ACTGTGGTTT
  841 CCAAATGTGT CAGTTTCATA GCCNGAANAA CGAGATCAGC ANCCTCNGTT CCNCNTACNC
  901 TTCNTTCTCA GTATTGNTTT TGNNNNNNNC NAANNNCNTC ANAAGCNNGN NCGANATNNN
961 NNNANNNNTN NNNNNNNNTT NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]