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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK388 (mClover-BSD)

A plasmid for construction of C-terminal mClover-BSD tagging donors.

Catalog number RDB16816
Resource name pMK388 (mClover-BSD)
Clone info. A plasmid for construction of C-terminal mClover-BSD tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Kanemaki, Masato |
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External Database
Aequorea victoria GFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Schedule A (GFP-A) [Link]
            Remarks
            Please use Schedule A (GFP-A) additionally.
            Distribution information (domestic users) [open/close]


            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB16816 pMK388 (mClover-BSD) DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Auxin Inducible Degron (AID) System (English text)
            Featured content Auxin Inducible Degron (AID) System (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB16816_A8Lpp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pUC-M13_-100
            Sequence file: RDB16816_A8Lpa.seq check
            >D06157B3_A8LP_2_pUC-M13_-100_A06_03_ABI24.ab1
                1 GGTCGGTAAG CGAATTAGTT GGGTACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG
               61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CGGATCCGGT
              121 GCAGGCGCCG CTAGCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC
              181 CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTCCGCGG CGAGGGCGAG
              241 GGCGATGCCA CCAACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC
              301 GTGCCCTGGC CCACCCTCGT GACCACCTTC GGCTACGGCG TGGCCTGCTT CAGCCGCTAC
              361 CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG
              421 GAGCGCACCA TCTCTTTCAA GGACGACGGT ACCTACAAGA CCCGCGCCGA GGTGAAGTTC
              481 GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC
              541 AACATCCTGG GGCACAAGCT GGAGTACAAC TTCAACAGCC ACAACGTCTA TATCACGGCC
              601 GACAAGCAGA AGAACGGCAT CAAGGCTAAC TTCAAGATCC GCCACAACGT TGAGGACGGC
              661 AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG
              721 CTGCCCGACA ACCACTACCT GAGCCATCAG TCCAAGCTGA GCAAAGACCC CAACGAGAAG
              781 CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATTACACA TGGCATGGAC
              841 GAGCTGTACA AGTAATCTAG ATAACTGATC ATAATCAGCC ATACCACATT TGTAGAGGTT
              901 TTACTTGCTT TAAAAAAACC TCCCACACCT CCCCCTGAAA CCTGAAACAT AAAAATGAAT
              961 GCAATTTGTT GTTGTTTAAC TTTGTTTTAA TTGCAGCTTT ATAATGGTTA CAATAAGCCA
             1021 TAGCCATCAC AAATTCACAA TTAAGCCATT TTTTTTCACT GCACTCTAGG TGGTGGCTTG
            1081 ATCCGAGCTC CAAC
            //
            Primer: reverse
            Sequence file: RDB16816_A8Lpb.seq check
            >D06157B3_A8LP_2_reverse_B06_06_ABI24.ab1
                1 CTGACCATTT CCCCTCACTA AGGGACAAAA GCTGGGTACC GGATCCTAGT GAACCTCTTC
               61 GAGGGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG GGTTCCGGAT
              121 CTCGACCAGC TTCTGATGGA ATTAGAACTT GGCAAAACAA TACTGAGAAT GAAGTGTATG
              181 TGGAACAGAG GCTGCTGATC TCGTTCTTCA GGCTATGAAA CTGACACATT TGGAAACCAC
              241 AGTACTTAGA ACCACAAAGT GGGAATCAAG AGAAAAACAA TGATCCCACG AGAGATCTAT
              301 AGATCTATAG ATCATGAGTG GGAGGAATGA GCTGGCCCTT AATTTGGTTT TGCTTGTTTA
              361 AATTATGATA TCCAACTATG AAACATTATC ATAAAGCAAT AGTAAAGAGC CTTCAGTAAA
              421 GAGCAGGCAT TTATCTAATC CCACCCCACC CCCACCCCCG TAGCTCCAAT CCTTCCATTC
              481 AAAATGTAGG TACTCTGTTC TCACCCTTCT TAACAAAGTA TGACAGGAAA AACTTCCATT
              541 TTAGTGGACA TCTTTATTGT TTAATAGATC ATCAATTTCT GCATCCCGGG GATCTGATAT
              601 CATCGATTAG CCCTCCCACA CATAACCAGA GGGCAGCAAT TCACGAATCC CAACTGCCGT
              661 CGGCTGTCCA TCACTGTCCT TCACTATGGC TTTGATCCCA GGATGCAGAT CGAGAAGCAC
              721 CTGTCGGCAC CGTCCGCAGG GGCTCAAGAT GCCCCTGTTC TCATTTCCGA TCGCGACGAT
              781 ACAAGTCAGG TTGCCAGCTG CCGCAGCAGC AGCAGTGCCC AGCACCACGA GTTCTGCACA
              841 AGGTCCCCCC AGTAAAATGA TATACATTGA CACCAGTGAA GATGCGGCCG TCGCTAGAGA
              901 GAGCTGCGCT GGCGACGCTG TAGTCTTCAG AGATGGGGGA TGCTGTTGAT TGTAGCCCGT
              961 TGCTCTTTCA ATGAGGGTGG GATTCTTTCT TGAGACAAAG GCTTGATCAT GATGGGCTCG
             1021 GAGATCTAGA TGAATGCAAG TCGAAGGTCC GAGATGAGGA GAGAAGTACC AGCGCCGCAG
            1081 GACCGATGCT G
            //
            Primer: PGK_N_rev
            Sequence file: RDB16816_A8Lpc.seq check
            >D06157B3_A8LP_2_PGK_N_rev_C06_09_ABI24.ab1
                1 CCAGGGGGCG GCTCCCTACC CGGTAGATTT CGAGGTCGAG ATCCTAAGCT TGGCTGGACG
               61 TAAACTCCTC TTCAGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG
              121 GGTTATTGAA TATGATCGAC GCGTTAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA
              181 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA
              241 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG
              301 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
              361 CTGATTATGA TCAGTTATCT AGATTACTTG TACAGCTCGT CCATGCCATG TGTAATCCCG
              421 GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC
              481 AGCTTGGACT GATGGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG
              541 GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCTCAAC GTTGTGGCGG
              601 ATCTTGAAGT TAGCCTTGAT GCCGTTCTTC TGCTTGTCGG CCGTGATATA GACGTTGTGG
              661 CTGTTGAAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG
              721 CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC
              781 TTGTAGGTAC CGTCGTCCTT GAAAGAGATG GTGCGCTCCT GGACGTAGCC TTCGGGCATG
              841 GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGCTGAA GCAGGCCACG
              901 CCGTAGCCGA AGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GGTGGGTGCA
              961 GATGAACTTC AGGGTCAGCT TGCCGTTGGG TGGGCATCGC CCTCGCCCTC GCCGCGGACG
             1021 CTGACTTGTG GCCGTTTAAC GTTCGGCGTC CAGCTCGAAC CAGGATGGCA TACCTTCGGT
            1081 GAACCAGCTC CCTCGACCCC CATG
            //
            Primer: PGKpro_F
            Sequence file: RDB16816_A8Lpd.seq check
            >D06157B3_A8LP_2_PGKpro_F_D06_12_ABI24.ab1
                1 ACCCCCGCCG TCTCTCTCTT CTCATCTCCG GGCCTTTCGA CCTGCATCCA TCTAGATCTC
               61 GAGCCACCAT GGCCAAGCCT TTGTCTCAAG AAGAATCCAC CCTCATTGAA AGAGCAACGG
              121 CTACAATCAA CAGCATCCCC ATCTCTGAAG ACTACAGCGT CGCCAGCGCA GCTCTCTCTA
              181 GCGACGGCCG CATCTTCACT GGTGTCAATG TATATCATTT TACTGGGGGA CCTTGTGCAG
              241 AACTCGTGGT GCTGGGCACT GCTGCTGCTG CGGCAGCTGG CAACCTGACT TGTATCGTCG
              301 CGATCGGAAA TGAGAACAGG GGCATCTTGA GCCCCTGCGG ACGGTGCCGA CAGGTGCTTC
              361 TCGATCTGCA TCCTGGGATC AAAGCCATAG TGAAGGACAG TGATGGACAG CCGACGGCAG
              421 TTGGGATTCG TGAATTGCTG CCCTCTGGTT ATGTGTGGGA GGGCTAATCG ATGATATCAG
              481 ATCCCCGGGA TGCAGAAATT GATGATCTAT TAAACAATAA AGATGTCCAC TAAAATGGAA
              541 GTTTTTCCTG TCATACTTTG TTAAGAAGGG TGAGAACAGA GTACCTACAT TTTGAATGGA
              601 AGGATTGGAG CTACGGGGGT GGGGGTGGGG TGGGATTAGA TAAATGCCTG CTCTTTACTG
              661 AAGGCTCTTT ACTATTGCTT TATGATAATG TTTCATAGTT GGATATCATA ATTTAAACAA
              721 GCAAAACCAA ATTAAGGGCC AGCTCATTCC TCCCACTCAT GATCTATAGA TCTATAGATC
              781 TCTCGTGGGA TCATTGTTTT TCTCTTGATT CCCACTTTGT GGTTCTAAGT ACTGTGGTTT
              841 CCAAATGTGT CAGTTTCATA GCCTGAAGAA CGAGATCAGC AGCCTCTGTT CCACATACAC
              901 TTCATTCTCA GTATTGTTTT TGCCAAGTTC TAATTCCATC AGAAGCTGGG TCGAGATCCC
              961 GGAAACCCTT AATATAACTT CGTATAATGT ATGCTATACG AAAGTTATTA GGTCCCTCGA
             1021 GAGGTTTCAC TAGGATCCGG GTACCCAAGC CTTTTGATCC CCATTAGTGA GGATATGGCG
            1081 CGGCTGGCGG TAAATTCAAT TTGGATCA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

            2021.11.02

            GNP_filter3_RDBDEP_html_211029.pl