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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK391 (mAID-BSD)

A plasmid for construction of C-terminal mAID-BSD tagging donors.

Catalog number RDB16819
Resource name pMK391 (mAID-BSD)
Clone info. A plasmid for construction of C-terminal mAID-BSD tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA
Depositor|Developer Kanemaki, Masato |
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External Database
Arabidopsis thaliana AtIAA17

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB16819 pMK391 (mAID-BSD) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16819_A8Lpp1-4.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pUC-M13_-100
          Region: T7 pro,linker, mAID,SV40 pA,loxP
          Sequence file: RDB16819_A8Lpd.seq check
          >D06157B6_A8LP_1_pUC-M13_-100_F05_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNT GGGNANGCNN GGGNTTTCCC NGTCNCGACG TTGTAAAACG
             61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CCCCGGGATC
            121 CGGTGCAGGC GCCAAGGAGA AGAGTGCTTG TCCTAAAGAT CCAGCCAAAC CTCCGGCCAA
            181 GGCACAAGTT GTGGGATGGC CACCGGTGAG ATCATACCGG AAGAACGTGA TGGTTTCCTG
            241 CCAAAAATCA AGCGGTGGCC CGGAGGCGGC GGCGTTCGTG AAGGTATCAA TGGACGGAGC
            301 ACCGTACTTG AGGAAAATCG ATTTGAGGAT GTATAAATAA TCTAGATAAC TGATCATAAT
            361 CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT
            421 GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA
            481 TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA
            541 TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAA CGCGTCGATC ATATTCAATA
            601 ACCCTTAATA TAACTTCGTA TAATGTATGC TATACGAAGT TATTAGGTCT GAAGAGGAGT
            661 TTACGTCCAG CCAAGCTTAN GATCTCGACC TCGAAATTCT ACCGGGTAGG GGAGGCGCTT
            721 TTCCCAAGGC AGTCTGGANC ATGCGCTTTA GCAGCCCCGC TGGGCACTTG GCGCTACACA
            781 AGTGGCCTCT GGNCTCGCAC ACATTCCACA TCCACCGGTA NGCGCCAACN GANTCCNNTC
            841 TTNGGNGGCC CCTTCGCGCC NCCTTCTANN NCTCCCCNAN NCANGAANTT NCCCCCCCNG
          901 CCCCNNANNN NGNNNCNNNN NNNANNNGNN NAANNGNNAN NTNNCNCNNN NNNNNNN
          //
          Primer: reverse
          Region: loxP,PGK ter, BSD
          Sequence file: RDB16819_A8Lpe.seq check
          >D06157B6_A8LP_1_pUC-M13_-100_F05_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNT GGGNANGCNN GGGNTTTCCC NGTCNCGACG TTGTAAAACG
             61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CCCCGGGATC
            121 CGGTGCAGGC GCCAAGGAGA AGAGTGCTTG TCCTAAAGAT CCAGCCAAAC CTCCGGCCAA
            181 GGCACAAGTT GTGGGATGGC CACCGGTGAG ATCATACCGG AAGAACGTGA TGGTTTCCTG
            241 CCAAAAATCA AGCGGTGGCC CGGAGGCGGC GGCGTTCGTG AAGGTATCAA TGGACGGAGC
            301 ACCGTACTTG AGGAAAATCG ATTTGAGGAT GTATAAATAA TCTAGATAAC TGATCATAAT
            361 CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT
            421 GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA
            481 TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA
            541 TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAA CGCGTCGATC ATATTCAATA
            601 ACCCTTAATA TAACTTCGTA TAATGTATGC TATACGAAGT TATTAGGTCT GAAGAGGAGT
            661 TTACGTCCAG CCAAGCTTAN GATCTCGACC TCGAAATTCT ACCGGGTAGG GGAGGCGCTT
            721 TTCCCAAGGC AGTCTGGANC ATGCGCTTTA GCAGCCCCGC TGGGCACTTG GCGCTACACA
            781 AGTGGCCTCT GGNCTCGCAC ACATTCCACA TCCACCGGTA NGCGCCAACN GANTCCNNTC
            841 TTNGGNGGCC CCTTCGCGCC NCCTTCTANN NCTCCCCNAN NCANGAANTT NCCCCCCCNG
          901 CCCCNNANNN NGNNNCNNNN NNNANNNGNN NAANNGNNAN NTNNCNCNNN NNNNNNN
          //
          Primer: PGKpro_F
          Region: BSD, PGK ter
          Sequence file: RDB16819_A8Lpf.seq check
          >D06157B6_A8LP_1_PGKpro_F_H05_23_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNTCTCCN GGCCTTTCGN NCTGCATCCN TCTAGATCTC
             61 GAGCCACCAT GGCCAAGCCT TTGTCTCAAG AAGAATCCAC CCTCATTGAA AGAGCAACGG
            121 CTACAATCAA CAGCATCCCC ATCTCTGAAG ACTACAGCGT CGCCAGCGCA GCTCTCTCTA
            181 GCGACGGCCG CATCTTCACT GGTGTCAATG TATATCATTT TACTGGGGGA CCTTGTGCAG
            241 AACTCGTGGT GCTGGGCACT GCTGCTGCTG CGGCAGCTGG CAACCTGACT TGTATCGTCG
            301 CGATCGGAAA TGAGAACAGG GGCATCTTGA GCCCCTGCGG ACGGTGCCGA CAGGTGCTTC
            361 TCGATCTGCA TCCTGGGATC AAAGCCATAG TGAAGGACAG TGATGGACAG CCGACGGCAG
            421 TTGGGATTCG TGAATTGCTG CCCTCTGGTT ATGTGTGGGA GGGCTAATCG ATGATATCAG
            481 ATCCCCGGGA TGCAGAAATT GATGATCTAT TAAACAATAA AGATGTCCAC TAAAATGGAA
            541 GTTTTTCCTG TCATACTTTG TTAAGAAGGG TGAGAACAGA GTACCTACAT TTTGAATGGA
            601 AGGATTGGAG CTACGGGGGT GGGGGTGGGG TGGGATTAGA TAAATGCCTG CTCTTTACTG
            661 AAGGCTCTTT ACTATTGCTT TATGATAATG TTTCATAGTT GGATATCATA ATTTAAACAA
            721 GCAAAACCAA ATTAAGGGCC AGCTCATTCC TCCCACTCAT GATCTATAGA TCTATAGATC
            781 TCTCGTGNNA TCATTGTTTT TCTCTTGATT CCCACTTTGT GGNTCTAAGT ACTGTGGTTT
            841 CCAAATGTGT CAGTTTTCAT AGCCNGAANA NNGANNTCAG CANCCTCTGT TCCNCNTACN
            901 CTTCATTCTC ANNATTGTTT NNNCNNNNTC TNANNNCNTC NNAANNCTNG NCNANNNNNN
          961 NNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

          2022.07.06

          GNP_filter3_RDBDEP_html_220520.pl