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pMK391 (mAID-BSD)

A plasmid for construction of C-terminal mAID-BSD tagging donors.

Catalog number RDB16819
Resource name pMK391 (mAID-BSD)
Clone info. A plasmid for construction of C-terminal mAID-BSD tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA
Depositor Kanemaki, Masato |
 
Other clones in our bank Arabidopsis thaliana AXR3 (KEGG orthology K14484) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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RDB16819 pMK391 (mAID-BSD) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16819_A8Lpp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 RDB16819_A8Lpa.seq
>D06157B6_A8LP_1_pUC-M13_-100_F05_17_ABI24.ab1
    1 GTCGGTCAAG CGATTAGGTT GGGTACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG
   61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CCCCGGGATC
  121 CGGTGCAGGC GCCAAGGAGA AGAGTGCTTG TCCTAAAGAT CCAGCCAAAC CTCCGGCCAA
  181 GGCACAAGTT GTGGGATGGC CACCGGTGAG ATCATACCGG AAGAACGTGA TGGTTTCCTG
  241 CCAAAAATCA AGCGGTGGCC CGGAGGCGGC GGCGTTCGTG AAGGTATCAA TGGACGGAGC
  301 ACCGTACTTG AGGAAAATCG ATTTGAGGAT GTATAAATAA TCTAGATAAC TGATCATAAT
  361 CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT
  421 GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA
  481 TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA
  541 TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAA CGCGTCGATC ATATTCAATA
  601 ACCCTTAATA TAACTTCGTA TAATGTATGC TATACGAAGT TATTAGGTCT GAAGAGGAGT
  661 TTACGTCCAG CCAAGCTTAG GATCTCGACC TCGAAATTCT ACCGGGTAGG GGAGGCGCTT
  721 TTCCCAAGGC AGTCTGGAGC ATGCGCTTTA GCAGCCCCGC TGGGCACTTG GCGCTACACA
  781 AGTGGCCTCT GGCCTCGCAC ACATTCCACA TCCACCGGTA GGCGCCAACC GACTCCGTTC
  841 TTTGGTGGCC CCTTCGCGCC ACCTTCTACT CCTCCCCTAG TCAGGAAGTT CCCCCCCCCG
  901 CCCCGCAGCT CGCGTCGTGC AGGACGTGAC AAATGGGAAA GTAGCACGTC TCACTAGTCT
  961 CGTGCAGATG GACAGCACCG CTGAGCAATG GAAGCGGGTA GGCCCTTTGG GGGCAGCGGA
 1021 CCAATAGCAG GCTTTTGCTC TCGCTTTCTG GGACTCAGAT GCTGGAGGGA TGGTCCCTGG
1081 GGGTGGGTCT TCACATGGGG ACG
//
Primer: reverse RDB16819_A8Lpb.seq
>D06157B6_A8LP_1_reverse_G05_20_ABI24.ab1
    1 CGAGCATTAA CCTCACTAAA GGGACAAAAG CTGGGTACCG GATCCTAGTG AACCTCTTCG
   61 AGGGACCTAA TAACTTCGTA TAGCATACAT TATACGAAGT TATATTAAGG GTTCCGGATC
  121 TCGACCAGCT TCTGATGGAA TTAGAACTTG GCAAAACAAT ACTGAGAATG AAGTGTATGT
  181 GGAACAGAGG CTGCTGATCT CGTTCTTCAG GCTATGAAAC TGACACATTT GGAAACCACA
  241 GTACTTAGAA CCACAAAGTG GGAATCAAGA GAAAAACAAT GATCCCACGA GAGATCTATA
  301 GATCTATAGA TCATGAGTGG GAGGAATGAG CTGGCCCTTA ATTTGGTTTT GCTTGTTTAA
  361 ATTATGATAT CCAACTATGA AACATTATCA TAAAGCAATA GTAAAGAGCC TTCAGTAAAG
  421 AGCAGGCATT TATCTAATCC CACCCCACCC CCACCCCCGT AGCTCCAATC CTTCCATTCA
  481 AAATGTAGGT ACTCTGTTCT CACCCTTCTT AACAAAGTAT GACAGGAAAA ACTTCCATTT
  541 TAGTGGACAT CTTTATTGTT TAATAGATCA TCAATTTCTG CATCCCGGGG ATCTGATATC
  601 ATCGATTAGC CCTCCCACAC ATAACCAGAG GGCAGCAATT CACGAATCCC AACTGCCGTC
  661 GGCTGTCCAT CACTGTCCTT CACTATGGCT TTGATCCCAG GATGCAGATC GAGAAGCACC
  721 TGTCGGCACC GTCCGCAGGG GCTCAAGATG CCCCTGTTCT CATTTCCGAT CGCGACGATA
  781 CAAGTCAGGT TGCCAGCTGC CGCAGCAGCA GCAGTGCCCA GCACCACGAG TTCTGCACAA
  841 GGTCCCCCCA GTAAAATGAT ATACATTGAC ACCAGTGAAG ATGCGGCCGT CGCTAGAGAG
  901 AGCTGCGCTG GCGACGCTGT AGTCTTCAGA GATGGGGGAT GCTGTTGATT GTAGCCGTTG
  961 CTCTTTCAAT GAGGTGGGAT TCTTTCTTTG AGACAAAACG CTTGGCCATG GATGGCTCGA
 1021 GATCTAGATG GAATGCAAGT CGAACGACCC GGAAGATGAG AAGAGGAGAC CAGCCGCCGA
1081 CCAGCACGGT GGCG
//
Primer: PGKpro_F RDB16819_A8Lpc.seq
>D06157B6_A8LP_1_PGKpro_F_H05_23_ABI24.ab1
    1 ACTCGCCCCG TATCTCTCTT CTCATCTCCG GGCCTTTCGA CCTGCATCCA TCTAGATCTC
   61 GAGCCACCAT GGCCAAGCCT TTGTCTCAAG AAGAATCCAC CCTCATTGAA AGAGCAACGG
  121 CTACAATCAA CAGCATCCCC ATCTCTGAAG ACTACAGCGT CGCCAGCGCA GCTCTCTCTA
  181 GCGACGGCCG CATCTTCACT GGTGTCAATG TATATCATTT TACTGGGGGA CCTTGTGCAG
  241 AACTCGTGGT GCTGGGCACT GCTGCTGCTG CGGCAGCTGG CAACCTGACT TGTATCGTCG
  301 CGATCGGAAA TGAGAACAGG GGCATCTTGA GCCCCTGCGG ACGGTGCCGA CAGGTGCTTC
  361 TCGATCTGCA TCCTGGGATC AAAGCCATAG TGAAGGACAG TGATGGACAG CCGACGGCAG
  421 TTGGGATTCG TGAATTGCTG CCCTCTGGTT ATGTGTGGGA GGGCTAATCG ATGATATCAG
  481 ATCCCCGGGA TGCAGAAATT GATGATCTAT TAAACAATAA AGATGTCCAC TAAAATGGAA
  541 GTTTTTCCTG TCATACTTTG TTAAGAAGGG TGAGAACAGA GTACCTACAT TTTGAATGGA
  601 AGGATTGGAG CTACGGGGGT GGGGGTGGGG TGGGATTAGA TAAATGCCTG CTCTTTACTG
  661 AAGGCTCTTT ACTATTGCTT TATGATAATG TTTCATAGTT GGATATCATA ATTTAAACAA
  721 GCAAAACCAA ATTAAGGGCC AGCTCATTCC TCCCACTCAT GATCTATAGA TCTATAGATC
  781 TCTCGTGGGA TCATTGTTTT TCTCTTGATT CCCACTTTGT GGTTCTAAGT ACTGTGGTTT
  841 CCAAATGTGT CAGTTTTCAT AGCCTGAAGA ACGAGATCAG CAGCCTCTGT TCCACATACA
  901 CTTCATTCTC AGTATTGTTT TGCCAAGTTC TAATTCCATC AGAAAGCTGG TCGAGATCCG
  961 GAACCCTTTA ATATAACTTC GTATATGTAT GCTATACGAA GTTATTTAGG TCCCTCGAGA
 1021 GGTTTCACTA GGATTCGGGT ACCAAGCTTT TGTTCCATTA GTGAGGATAG TGCACGGCTG
1081 ACGTTAATTC AATGGGATTC CA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

2019.07.18

GNP_filter3_RDBDEP_html_190432.pl