A plasmid for construction of C-terminal mCherry tagging donors.
Alternative name | mCherry2-Bsr, 974 |
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Clone info. | A plasmid for construction of C-terminal mCherry tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone. |
Vector backbone | pBluescriptII KS- (Plasmid) |
Size of vector backbone | 3.0 kb |
Selectable markers | Am^r |
Gene/insert name | Discosoma sp. RFP cDNA |
Depositor|Developer | Kanemaki, Masato | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] In order to receive this biological resource, please request the Exclusive MTA to us. Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB13925 | pMK282 (mCherry2-Bsr) | DNA solution |
Materials & Methods section:
The pMK282 (mCherry2-Bsr) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13925). |
Reference section:
Natsume, T., Kiyomitsu, T., Saga, Y., Kanemaki, M.T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13925_A6B8p1-4.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: M13_-40 (Pr0410) Region: T7 pro,insert 5'(Linker,mCherry2 5') Sequence file: RDB13925_A6B8e.seq |
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>D03070A09_A6B8_1_M13(-40)_F02_17.ab1 1 NNNNNNNNNN NNNNNNCNNA NNNGNNTNNC NNTAGGGCGA ATTGGAGCTC GGATCCGGTG 61 CAGGCGCCGC TAGCATGGTG AGCAAGGGCG AGGAGGATAA CATGGCCATC ATCAAGGAGT 121 TCATGCGCTT CAAGGTGCAC ATGGAGGGCT CCGTGAACGG CCACGAGTTC GAGATCGAGG 181 GCGAGGGCGA GGGCCGCCCC TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG 241 GTGGCCCCCT GCCCTTCGCC TGGGACATCC TGTCCCCTCA GTTCATGTAC GGCTCCAAGG 301 CCTACGTGAA GCACCCCGCC GACATCCCCG ACTACTTGAA GCTGTCCTTC CCCGAGGGCT 361 TCAATTGGGA GCGCGTGATG AACTTCGAGG ACGGCGGCGT GGTGACCGTG ACCCAGGACT 421 CCTCCCTGCA GGACGGCGAG TTCATCTACA AGGTGAAGCT GCGCGGCACC AACTTCCCCT 481 CCGACGGCCC CGTAATGCAG TGTCGTACCA TGGGCTGGGA GGCCTCCACT GAGCGGATGT 541 ACCCCGAGGA CGGCGCCCTG AAGGGCGAGA TCAAGCAGAG GCTGAAGCTG AAGGACGGCG 601 GCCACTACGA CGCTGAGGTC AAGACCACCT ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG 661 GCGCCTACAA CGTCGACATC AAGTTGGACA TCCTTTCCCA CAACGAGGAC TACACCATCG 721 TGGAACAGTA CGAACGCGCC GAGGGCCGCC ACTCCACCGG CGGCATGGAC GAGCTGTACA 781 AGTAATCTAG ATAACTGATC ATAATCAGCC ATACCACATT TGTAGANGNT TTACTTGCTT 841 NNAAAANNCT CCCACACCTN CCCCTGAACC TGAANNATAA AATGAATGCN ATTGTTGTTG 901 NTAANTTGTT TATTGCANCT TATAANNGNT TACAAANNAA GCAATAGCAT CNCAAATTTC 961 ACAAANNAAN NCATTTTTTT TNNCTGNNTT CNANNNNNGG TTTGNCCAAN NNNNNCNNNG 1021 NNNNNNNNNN // |
Primer: M13R (Pr0160) Region: insert 3'(loxP,mPGK ter) Sequence file: RDB13925_A6B8f.seq |
>D03070A09_A6B8_1_M13R_F01_16.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNGGNA NNAAAGCTGG GTACCGGATC CTAGTGAACC 61 TCTTCGAGGG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA TTAAGGGTTC 121 CGGATCTCGA CCAGCTTCTG ATGGAATTAG AACTTGGCAA AACAATACTG AGAATGAAGT 181 GTATGTGGAA CAGAGGCTGC TGATCTCGTT CTTCAGGCTA TGAAACTGAC ACATTTGGAA 241 ACCACAGTAC TTAGAACCAC AAAGTGGGAA TCAAGAGAAA AACAATGATC CCACGAGAGA 301 TCTATAGATC TATAGATCAT GAGTGGGAGG AATGAGCTGG CCCTTAATTT GGTTTTGCTT 361 GTTTAAATTA TGATATCCAA CTATGAAACA TTATCATAAA GCAATAGTAA AGAGCCTTCA 421 GTAAAGAGCA GGCATTTATC TAATCCCACC CCACCCCCAC CCCCGTAGCT CCAATCCTTC 481 CATTCAAAAT GTAGGTACTC TGTTCTCACC CTTCTTAACA AAGTATGACA GGAAAAACTT 541 CCATTTTAGT GGACATCTTT ATTGTTTAAT AGATCATCAA TTTCTGCATC CCGGGGATCT 601 GATATCATCG ATGCATGGGG TCGTGCGCTC CTTTCGGTCG GGCGCTGCGG GTCGTGGGGC 661 GGGCGGCTAG CTTAATTTCG GGTANNNNNG ANTGGAATGA GTTCTTCAAT CNTAGTTTTG 721 ACTANNTTGC CATTCATTTC TATTAACACA NNNNNNNCTG GTGCATAGTC TGAAANTCAA 781 CNNCCCTACA CNTACCACAA NNGACTTNNC CNNCTCGAAT NNNNTCTATC NACNTTCGTC 841 NNANNAANGN TGNNNNNAAN ANNCTANNAN TCGNNGNNNN ANTNCNTTTT GNCCANTNCN 901 AAAACNNGNN NNNNNNNN // |
Primer: PGK_N_rev (Pr0243) Region: insert mid(loxP,SV40pA,mCherry2 3') Sequence file: RDB13925_A6B8g.seq |
>D03070A09_A6B8_1_PGK-N-rev_D06_12.ab1 1 NNNNNNNNNN NNNNNNNGNN NANTTCGAGG TCGAGNTCCT AAGCTTGGCT GGACGTAAAC 61 TCCTCTTCAG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA TTAAGGGTTA 121 TTGAATATGA TCGACGCGTT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG 181 CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT 241 ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG 301 GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TATGGCTGAT 361 TATGATCAGT TATCTAGATT ACTTGTACAG CTCGTCCATG CCGCCGGTGG AGTGGCGGCC 421 CTCGGCGCGT TCGTACTGTT CCACGATGGT GTAGTCCTCG TTGTGGGAAA GGATGTCCAA 481 CTTGATGTCG ACGTTGTAGG CGCCGGGCAG CTGCACGGGC TTCTTGGCCT TGTAGGTGGT 541 CTTGACCTCA GCGTCGTAGT GGCCGCCGTC CTTCAGCTTC AGCCTCTGCT TGATCTCGCC 601 CTTCAGGGCG CCGTCCTCGG GGTACATCCG CTCAGTGGAG GCCTCCCAGC CCATGGTACG 661 ACACTGCATT ACGGGGCCGT CGGANGGGAA GTTGGTGCCG CGCAGCTTCA CCTTGTAGAT 721 GAACTCGCCG TCCTGCAGGG AGGAGTCCTG GGTCACGGTC ACCACGCCGC CGTCCTCGAA 781 NTTCATCACG CGCTCNCNAT TGAAGCCCTC NGGGAANGAC AGCTTCAAGT AGTCNGGGAN 841 GTCNGCGGGG TGCTTCACGT NNNCCTTGGA NCCGTACATG ANNTGANGGG ANANGNNGTN 901 CCANGCGANN GGNNNGGGGC NACCCTNNGT CACCNNCANC TTNNNNGTCT GGGNGNCCNN 961 GNNNGGNCNG NNCNNCNNNN NCNNCCNNNN NNNNNN // |
Primer: PGKpro_F (Pr0249) Region: insert mid(bsr,mPGK ter) Sequence file: RDB13925_A6B8h.seq |
>D03070A09_A6B8_1_PGK-proF_D07_10.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNTCCNNGC CTTTCGACCT GCATCCNTCT AGATCTCGAG 61 CAGCTGAAGC TTACCGCTAG CATGAAAACA TTTAACATTT CTCAACAAGA TCTAGAATTA 121 GTAGAAGTAG CGACAGAGAA GATTACAATG CTTTATGAGG ATAATAAACA TCATGTGGGA 181 GCGGCAATTC GTACGAAAAC AGGAGAAATC ATTTCGGCAG TACATATTGA AGCGTATATA 241 GGACGAGTAA CTGTTTGTGC AGAAGCCATT GCGATTGGTA GTGCAGTTTC GAATGGACAA 301 AAGGATTTTG ACACGATTGT AGCTGTTAGA CACCCTTATT CTGACGAAGT AGATAGAAGT 361 ATTCGAGTGG TAAGTCCTTG TGGTATGTGT AGGGAGTTGA TTTCAGACTA TGCACCAGAT 421 TGTTTTGTGT TAATAGAAAT GAATGGCAAG TTAGTCAAAA CTACGATTGA AGAACTCATT 481 CCACTCAAAT ATACCCGAAA TTAAGCTAGC CGCCCGCCCC ACGACCCGCA GCGCCCGACC 541 GAAAGGAGCG CACGACCCCA TGCATCGATG ATATCAGATC CCCGGGATGC AGAAATTGAT 601 GATCTATTAA ACAATAAAGA TGTCCACTAA AATGGAAGTT TTTCCTGTCA TACTTTGTTA 661 AGAAGGGTGA GAACAGAGTA CCTACATTTT GAATGGAAGG ANTGGAGCTA CGGGGGTGGG 721 GGTGGGGTGG GATTAGATAA ATGCCTGCTC TTTACTGAAG GNTCTTTACT ATTGCTTTAT 781 GATAATGTTT CATAGTTGGA TATCATAATT TNAACAAGCA AAACCNANTT AANGGNCAGC 841 TCATTNCTCC CACTCATGAT CNATAGATCT ATAGATCTCT CGNGGGATCA TNGNTTTTTC 901 TCTTGANTCC CACTTTGTGG TTCNAAGTAC TGTGGTTTCC AAATGTGTCA GTTTCATAGN 961 CNTGNAGAAN GAGATNANNA GCCNNNNNTN NNNNTANNNT TCNNNNNCAN NNNNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP] |
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