Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK282 (mCherry2-Bsr)

A plasmid for construction of C-terminal mCherry tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose)[Link]
Remarks	An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required. The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose).

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 専用MTA (Takara Bio USA, Inc. FPs, 英語版のみ、非営利機関による非営利学術研究用)[Link]
備考	クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。 本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。専用MTA（Takara Bio USA, Inc. FPs, 英語版のみ、非営利機関による非営利学術研究用）をご参照ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13925_A6B8p1_v2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB13925_A6B8a_2.seq
>D03070A09_A6B8_1_M13(-40)_F02_17.ab1     1 CCGCCAGCTA GCGGCGCGTA TACGACTCAC TATAGGGCGA ATTGGAGCTC GGATCCGGTG    61 CAGGCGCCGC TAGCATGGTG AGCAAGGGCG AGGAGGATAA CATGGCCATC ATCAAGGAGT   121 TCATGCGCTT CAAGGTGCAC ATGGAGGGCT CCGTGAACGG CCACGAGTTC GAGATCGAGG   181 GCGAGGGCGA GGGCCGCCCC TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG   241 GTGGCCCCCT GCCCTTCGCC TGGGACATCC TGTCCCCTCA GTTCATGTAC GGCTCCAAGG   301 CCTACGTGAA GCACCCCGCC GACATCCCCG ACTACTTGAA GCTGTCCTTC CCCGAGGGCT   361 TCAATTGGGA GCGCGTGATG AACTTCGAGG ACGGCGGCGT GGTGACCGTG ACCCAGGACT   421 CCTCCCTGCA GGACGGCGAG TTCATCTACA AGGTGAAGCT GCGCGGCACC AACTTCCCCT   481 CCGACGGCCC CGTAATGCAG TGTCGTACCA TGGGCTGGGA GGCCTCCACT GAGCGGATGT   541 ACCCCGAGGA CGGCGCCCTG AAGGGCGAGA TCAAGCAGAG GCTGAAGCTG AAGGACGGCG   601 GCCACTACGA CGCTGAGGTC AAGACCACCT ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG   661 GCGCCTACAA CGTCGACATC AAGTTGGACA TCCTTTCCCA CAACGAGGAC TACACCATCG   721 TGGAACAGTA CGAACGCGCC GAGGGCCGCC ACTCCACCGG CGGCATGGAC GAGCTGTACA   781 AGTAATCTAG ATAACTGATC ATAATCAGCC ATACCACATT TGTAGAGGTT TTACTTGCTT   841 TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG   901 TTAACTTGTT TATTGCAGCT TATAATGGGT TACAAATAAA GCAATAGCAT CACAAATTTC   961 ACAAATAAAA GCATTTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG  1021 TATCTTAACG CGTCGATCAT ATTCAATAAA CCCTTTAATA TTACTTCGTA TAATGTATGC  1081 TATACGAAGT ATTAAGTCTG GAAGAAGGAG TTTACGTTCC AGCCAAGCTA GAATCCTCGG 1141 ACCTCCGAAA TCCTACCGGC TAAGGGAAGG CCGCTTTTTA CA //
Primer: PGKpro_F Sequence file: RDB13925_A6B8b_2.seq
>D03070A09_A6B8_1_PGK-proF_D07_10.ab1     1 ATCGGGCGTA CTCTCTTCTC ATCTCCGGGC CTTTCGACCT GCATCCATCT AGATCTCGAG    61 CAGCTGAAGC TTACCGCTAG CATGAAAACA TTTAACATTT CTCAACAAGA TCTAGAATTA   121 GTAGAAGTAG CGACAGAGAA GATTACAATG CTTTATGAGG ATAATAAACA TCATGTGGGA   181 GCGGCAATTC GTACGAAAAC AGGAGAAATC ATTTCGGCAG TACATATTGA AGCGTATATA   241 GGACGAGTAA CTGTTTGTGC AGAAGCCATT GCGATTGGTA GTGCAGTTTC GAATGGACAA   301 AAGGATTTTG ACACGATTGT AGCTGTTAGA CACCCTTATT CTGACGAAGT AGATAGAAGT   361 ATTCGAGTGG TAAGTCCTTG TGGTATGTGT AGGGAGTTGA TTTCAGACTA TGCACCAGAT   421 TGTTTTGTGT TAATAGAAAT GAATGGCAAG TTAGTCAAAA CTACGATTGA AGAACTCATT   481 CCACTCAAAT ATACCCGAAA TTAAGCTAGC CGCCCGCCCC ACGACCCGCA GCGCCCGACC   541 GAAAGGAGCG CACGACCCCA TGCATCGATG ATATCAGATC CCCGGGATGC AGAAATTGAT   601 GATCTATTAA ACAATAAAGA TGTCCACTAA AATGGAAGTT TTTCCTGTCA TACTTTGTTA   661 AGAAGGGTGA GAACAGAGTA CCTACATTTT GAATGGAAGG ATTGGAGCTA CGGGGGTGGG   721 GGTGGGGTGG GATTAGATAA ATGCCTGCTC TTTACTGAAG GCTCTTTACT ATTGCTTTAT   781 GATAATGTTT CATAGTTGGA TATCATAATT TAAACAAGCA AAACCAAATT AAGGGCCAGC   841 TCATTCCTCC CACTCATGAT CTATAGATCT ATAGATCTCT CGTGGGATCA TTGTTTTTTC   901 TCTTGATTCC CACTTTGTGG TTCTAAGTAC TGTGGTTTCC AAATGTGTCA GTTTCATAGC   961 CCTGAAGAAC GAGATCAGCA GCCTCTGTTC CACATACACT TCATTCTCAG TATTGTTTTG  1021 CCAAGGTTCT AATTTCCAAT CAGAAGCTGG GTCGAGATCC GGAACCCTTT AAATAATACT  1081 TCGTATATGG TATGCTAATA CGAAAGTAAT AGGTTCCCTT CGAAGAGGTC ACTTAGAATT 1141 CCAGGTAACA GCTTGATCCC CATTGATGAA AGAGT //
Primer: PGK_N_rev Sequence file: RDB13925_A6B8c_2.seq
>D03070A09_A6B8_1_PGK-N-rev_D06_12.ab1     1 CCTGACGCTC CCTACCGGTA GATTTCGAGG TCGAGATCCT AAGCTTGGCT GGACGTAAAC    61 TCCTCTTCAG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA TTAAGGGTTA   121 TTGAATATGA TCGACGCGTT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG   181 CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT   241 ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG   301 GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TATGGCTGAT   361 TATGATCAGT TATCTAGATT ACTTGTACAG CTCGTCCATG CCGCCGGTGG AGTGGCGGCC   421 CTCGGCGCGT TCGTACTGTT CCACGATGGT GTAGTCCTCG TTGTGGGAAA GGATGTCCAA   481 CTTGATGTCG ACGTTGTAGG CGCCGGGCAG CTGCACGGGC TTCTTGGCCT TGTAGGTGGT   541 CTTGACCTCA GCGTCGTAGT GGCCGCCGTC CTTCAGCTTC AGCCTCTGCT TGATCTCGCC   601 CTTCAGGGCG CCGTCCTCGG GGTACATCCG CTCAGTGGAG GCCTCCCAGC CCATGGTACG   661 ACACTGCATT ACGGGGCCGT CGGAGGGGAA GTTGGTGCCG CGCAGCTTCA CCTTGTAGAT   721 GAACTCGCCG TCCTGCAGGG AGGAGTCCTG GGTCACGGTC ACCACGCCGC CGTCCTCGAA   781 GTTCATCACG CGCTCCCAAT TGAAGCCCTC GGGGAAGGAC AGCTTCAAGT AGTCGGGGAT   841 GTCGGCGGGG TGCTTCACGT AGGCCTTGGA GCCGTACATG AACTGAGGGG ACAGGATGTC   901 CCAGGCGAAG GGCAGGGGGC CACCCTTGGT CACCTTCAGC TTGGCGGTCT GGGTGCCCTC   961 GTAGGGGCGG GCCCTCGCCC TCGCCCTCGA TCTCGAACTC GTGGCCGTTC ACGGAGCCCT  1021 CCATGTGCAC CTTTGAAAGC GCATGACTCC TTGATGATTG GCATGATATC CCTCTTCGCC  1081 TGCTCACAAT GCTAGCCGGC GCTGCACGGA ATCCGAGCTC AATCGGCCTA TTAAGGGTGA 1141 AGTTCGTTAT ATACGCGCGG C //
Primer: M13R Sequence file: RDB13925_A6B8d_2.seq
>D03070A09_A6B8_1_M13R_F01_16.ab1     1 ATTCGCGGCA TTAACCCTCA CTAAAGGGAA CAAAAGCTGG GTACCGGATC CTAGTGAACC    61 TCTTCGAGGG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA TTAAGGGTTC   121 CGGATCTCGA CCAGCTTCTG ATGGAATTAG AACTTGGCAA AACAATACTG AGAATGAAGT   181 GTATGTGGAA CAGAGGCTGC TGATCTCGTT CTTCAGGCTA TGAAACTGAC ACATTTGGAA   241 ACCACAGTAC TTAGAACCAC AAAGTGGGAA TCAAGAGAAA AACAATGATC CCACGAGAGA   301 TCTATAGATC TATAGATCAT GAGTGGGAGG AATGAGCTGG CCCTTAATTT GGTTTTGCTT   361 GTTTAAATTA TGATATCCAA CTATGAAACA TTATCATAAA GCAATAGTAA AGAGCCTTCA   421 GTAAAGAGCA GGCATTTATC TAATCCCACC CCACCCCCAC CCCCGTAGCT CCAATCCTTC   481 CATTCAAAAT GTAGGTACTC TGTTCTCACC CTTCTTAACA AAGTATGACA GGAAAAACTT   541 CCATTTTAGT GGACATCTTT ATTGTTTAAT AGATCATCAA TTTCTGCATC CCGGGGATCT   601 GATATCATCG ATGCATGGGG TCGTGCGCTC CTTTCGGTCG GGCGCTGCGG GTCGTGGGGC   661 GGGCGGCTAG CTTAATTTCG GGTATATTTG AGTGGAATGA GTTCTTCAAT CGTAGTTTTG   721 ACTAACTTGC CATTCATTTC TATTAACACA AAACAATCTG GTGCATAGTC TGAAAATCAA   781 CTCCCCTACA CATACCACAA GGGACTTACC CAACTCGAAT ACCTTCTATC TACCTTCGTC   841 AGATTAAGGG TGTCCTTAAC AGCCTACCAA TCGTTGTCAA AATCCCTTTT GTCCATTTCG   901 AAAACTTGCC ACTACCAATC GCAATGGCTT CCTGCCAAAA CCAGTTACTC GTCCCAATAT   961 ACGCTTCAAT ATGTACCTGC CGAAATGATT TCCCCCTGTT TTTCGTAGAA ATTGGCCCGC 1021 TCCCCACCTG GAT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.02.09

GNP_filter3_RDBDEP_html_220112.pl