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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK282 (mCherry2-Bsr) (#RDB13925)

A plasmid for construction of C-terminal mCherry tagging donors.

Alternative name mCherry2-Bsr, 974
Clone info. A plasmid for construction of C-terminal mCherry tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone.
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Gene/insert name Discosoma sp. RFP cDNA
Depositor|Developer Kanemaki, Masato |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。

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RDB13925 pMK282 (mCherry2-Bsr) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMK282 (mCherry2-Bsr) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13925).

Reference section:

Natsume, T., Kiyomitsu, T., Saga, Y., Kanemaki, M.T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13925_A6B8p1-4.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410)
Region: T7 pro,insert 5'(Linker,mCherry2 5')
Sequence file: RDB13925_A6B8e.seq check
>D03070A09_A6B8_1_M13(-40)_F02_17.ab1
    1 NNNNNNNNNN NNNNNNCNNA NNNGNNTNNC NNTAGGGCGA ATTGGAGCTC GGATCCGGTG
   61 CAGGCGCCGC TAGCATGGTG AGCAAGGGCG AGGAGGATAA CATGGCCATC ATCAAGGAGT
  121 TCATGCGCTT CAAGGTGCAC ATGGAGGGCT CCGTGAACGG CCACGAGTTC GAGATCGAGG
  181 GCGAGGGCGA GGGCCGCCCC TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG
  241 GTGGCCCCCT GCCCTTCGCC TGGGACATCC TGTCCCCTCA GTTCATGTAC GGCTCCAAGG
  301 CCTACGTGAA GCACCCCGCC GACATCCCCG ACTACTTGAA GCTGTCCTTC CCCGAGGGCT
  361 TCAATTGGGA GCGCGTGATG AACTTCGAGG ACGGCGGCGT GGTGACCGTG ACCCAGGACT
  421 CCTCCCTGCA GGACGGCGAG TTCATCTACA AGGTGAAGCT GCGCGGCACC AACTTCCCCT
  481 CCGACGGCCC CGTAATGCAG TGTCGTACCA TGGGCTGGGA GGCCTCCACT GAGCGGATGT
  541 ACCCCGAGGA CGGCGCCCTG AAGGGCGAGA TCAAGCAGAG GCTGAAGCTG AAGGACGGCG
  601 GCCACTACGA CGCTGAGGTC AAGACCACCT ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG
  661 GCGCCTACAA CGTCGACATC AAGTTGGACA TCCTTTCCCA CAACGAGGAC TACACCATCG
  721 TGGAACAGTA CGAACGCGCC GAGGGCCGCC ACTCCACCGG CGGCATGGAC GAGCTGTACA
  781 AGTAATCTAG ATAACTGATC ATAATCAGCC ATACCACATT TGTAGANGNT TTACTTGCTT
  841 NNAAAANNCT CCCACACCTN CCCCTGAACC TGAANNATAA AATGAATGCN ATTGTTGTTG
  901 NTAANTTGTT TATTGCANCT TATAANNGNT TACAAANNAA GCAATAGCAT CNCAAATTTC
  961 ACAAANNAAN NCATTTTTTT TNNCTGNNTT CNANNNNNGG TTTGNCCAAN NNNNNCNNNG
1021 NNNNNNNNNN
//
Primer: M13R (Pr0160)
Region: insert 3'(loxP,mPGK ter)
Sequence file: RDB13925_A6B8f.seq check
>D03070A09_A6B8_1_M13R_F01_16.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNGGNA NNAAAGCTGG GTACCGGATC CTAGTGAACC
   61 TCTTCGAGGG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA TTAAGGGTTC
  121 CGGATCTCGA CCAGCTTCTG ATGGAATTAG AACTTGGCAA AACAATACTG AGAATGAAGT
  181 GTATGTGGAA CAGAGGCTGC TGATCTCGTT CTTCAGGCTA TGAAACTGAC ACATTTGGAA
  241 ACCACAGTAC TTAGAACCAC AAAGTGGGAA TCAAGAGAAA AACAATGATC CCACGAGAGA
  301 TCTATAGATC TATAGATCAT GAGTGGGAGG AATGAGCTGG CCCTTAATTT GGTTTTGCTT
  361 GTTTAAATTA TGATATCCAA CTATGAAACA TTATCATAAA GCAATAGTAA AGAGCCTTCA
  421 GTAAAGAGCA GGCATTTATC TAATCCCACC CCACCCCCAC CCCCGTAGCT CCAATCCTTC
  481 CATTCAAAAT GTAGGTACTC TGTTCTCACC CTTCTTAACA AAGTATGACA GGAAAAACTT
  541 CCATTTTAGT GGACATCTTT ATTGTTTAAT AGATCATCAA TTTCTGCATC CCGGGGATCT
  601 GATATCATCG ATGCATGGGG TCGTGCGCTC CTTTCGGTCG GGCGCTGCGG GTCGTGGGGC
  661 GGGCGGCTAG CTTAATTTCG GGTANNNNNG ANTGGAATGA GTTCTTCAAT CNTAGTTTTG
  721 ACTANNTTGC CATTCATTTC TATTAACACA NNNNNNNCTG GTGCATAGTC TGAAANTCAA
  781 CNNCCCTACA CNTACCACAA NNGACTTNNC CNNCTCGAAT NNNNTCTATC NACNTTCGTC
  841 NNANNAANGN TGNNNNNAAN ANNCTANNAN TCGNNGNNNN ANTNCNTTTT GNCCANTNCN
901 AAAACNNGNN NNNNNNNN
//
Primer: PGK_N_rev (Pr0243)
Region: insert mid(loxP,SV40pA,mCherry2 3')
Sequence file: RDB13925_A6B8g.seq check
>D03070A09_A6B8_1_PGK-N-rev_D06_12.ab1
    1 NNNNNNNNNN NNNNNNNGNN NANTTCGAGG TCGAGNTCCT AAGCTTGGCT GGACGTAAAC
   61 TCCTCTTCAG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA TTAAGGGTTA
  121 TTGAATATGA TCGACGCGTT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG
  181 CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT
  241 ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG
  301 GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TATGGCTGAT
  361 TATGATCAGT TATCTAGATT ACTTGTACAG CTCGTCCATG CCGCCGGTGG AGTGGCGGCC
  421 CTCGGCGCGT TCGTACTGTT CCACGATGGT GTAGTCCTCG TTGTGGGAAA GGATGTCCAA
  481 CTTGATGTCG ACGTTGTAGG CGCCGGGCAG CTGCACGGGC TTCTTGGCCT TGTAGGTGGT
  541 CTTGACCTCA GCGTCGTAGT GGCCGCCGTC CTTCAGCTTC AGCCTCTGCT TGATCTCGCC
  601 CTTCAGGGCG CCGTCCTCGG GGTACATCCG CTCAGTGGAG GCCTCCCAGC CCATGGTACG
  661 ACACTGCATT ACGGGGCCGT CGGANGGGAA GTTGGTGCCG CGCAGCTTCA CCTTGTAGAT
  721 GAACTCGCCG TCCTGCAGGG AGGAGTCCTG GGTCACGGTC ACCACGCCGC CGTCCTCGAA
  781 NTTCATCACG CGCTCNCNAT TGAAGCCCTC NGGGAANGAC AGCTTCAAGT AGTCNGGGAN
  841 GTCNGCGGGG TGCTTCACGT NNNCCTTGGA NCCGTACATG ANNTGANGGG ANANGNNGTN
  901 CCANGCGANN GGNNNGGGGC NACCCTNNGT CACCNNCANC TTNNNNGTCT GGGNGNCCNN
961 GNNNGGNCNG NNCNNCNNNN NCNNCCNNNN NNNNNN
//
Primer: PGKpro_F (Pr0249)
Region: insert mid(bsr,mPGK ter)
Sequence file: RDB13925_A6B8h.seq check
>D03070A09_A6B8_1_PGK-proF_D07_10.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTCCNNGC CTTTCGACCT GCATCCNTCT AGATCTCGAG
   61 CAGCTGAAGC TTACCGCTAG CATGAAAACA TTTAACATTT CTCAACAAGA TCTAGAATTA
  121 GTAGAAGTAG CGACAGAGAA GATTACAATG CTTTATGAGG ATAATAAACA TCATGTGGGA
  181 GCGGCAATTC GTACGAAAAC AGGAGAAATC ATTTCGGCAG TACATATTGA AGCGTATATA
  241 GGACGAGTAA CTGTTTGTGC AGAAGCCATT GCGATTGGTA GTGCAGTTTC GAATGGACAA
  301 AAGGATTTTG ACACGATTGT AGCTGTTAGA CACCCTTATT CTGACGAAGT AGATAGAAGT
  361 ATTCGAGTGG TAAGTCCTTG TGGTATGTGT AGGGAGTTGA TTTCAGACTA TGCACCAGAT
  421 TGTTTTGTGT TAATAGAAAT GAATGGCAAG TTAGTCAAAA CTACGATTGA AGAACTCATT
  481 CCACTCAAAT ATACCCGAAA TTAAGCTAGC CGCCCGCCCC ACGACCCGCA GCGCCCGACC
  541 GAAAGGAGCG CACGACCCCA TGCATCGATG ATATCAGATC CCCGGGATGC AGAAATTGAT
  601 GATCTATTAA ACAATAAAGA TGTCCACTAA AATGGAAGTT TTTCCTGTCA TACTTTGTTA
  661 AGAAGGGTGA GAACAGAGTA CCTACATTTT GAATGGAAGG ANTGGAGCTA CGGGGGTGGG
  721 GGTGGGGTGG GATTAGATAA ATGCCTGCTC TTTACTGAAG GNTCTTTACT ATTGCTTTAT
  781 GATAATGTTT CATAGTTGGA TATCATAATT TNAACAAGCA AAACCNANTT AANGGNCAGC
  841 TCATTNCTCC CACTCATGAT CNATAGATCT ATAGATCTCT CGNGGGATCA TNGNTTTTTC
  901 TCTTGANTCC CACTTTGTGG TTCNAAGTAC TGTGGTTTCC AAATGTGTCA GTTTCATAGN
961 CNTGNAGAAN GAGATNANNA GCCNNNNNTN NNNNTANNNT TCNNNNNCAN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP]