RDB13925 - pMK282 (mCherry2-Bsr)

Resource data sheet
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pMK282 (mCherry2-Bsr)

A plasmid for construction of C-terminal mCherry tagging donors.

Catalog number	RDB13925
Resource name	pMK282 (mCherry2-Bsr)
Alternative name	mCherry2-Bsr, 974
Clone info.	A plasmid for construction of C-terminal mCherry tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone.
Vector backbone	pBluescriptII KS- (Plasmid)
Size of vector backbone	3.0 kb
Selectable markers	Am^r
Gene/insert name	Discosoma sp. RFP cDNA
Depositor	Kanemaki, Masato \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

Ordering Please visit Information of Request for Distribution.
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Exclusive MTA [Link]

Remarks An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required.
Please use the Exclusive MTA (Clontech FPs).

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Exclusive MTA [Link]
Remarks	An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required. Please use the Exclusive MTA (Clontech FPs).

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 専用MTA（英語版のみ）[Link]
備考	クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。専用MTA（Clontech FPs, 英語版のみ）をお使いください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13925	pMK282 (mCherry2-Bsr)	DNA solution

cDNA Request [in Japanese] [in English]

References and tips

Electronic file

Featured content	Auxin Inducible Degron (AID) System (English text)
Featured content	Auxin Inducible Degron (AID) System (Japanese text)
Featured content	Genome Editing (English text)
Featured content	Genome Editing (Japanese text)
Featured content	Empty Backbone (English text)
Featured content	Empty Backbone (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB13925_A6B8p1_v2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40	RDB13925_A6B8a_2.seq
>D03070A09_A6B8_1_M13(-40)_F02_17.ab1 1 CCGCCAGCTA GCGGCGCGTA TACGACTCAC TATAGGGCGA ATTGGAGCTC GGATCCGGTG 61 CAGGCGCCGC TAGCATGGTG AGCAAGGGCG AGGAGGATAA CATGGCCATC ATCAAGGAGT 121 TCATGCGCTT CAAGGTGCAC ATGGAGGGCT CCGTGAACGG CCACGAGTTC GAGATCGAGG 181 GCGAGGGCGA GGGCCGCCCC TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG 241 GTGGCCCCCT GCCCTTCGCC TGGGACATCC TGTCCCCTCA GTTCATGTAC GGCTCCAAGG 301 CCTACGTGAA GCACCCCGCC GACATCCCCG ACTACTTGAA GCTGTCCTTC CCCGAGGGCT 361 TCAATTGGGA GCGCGTGATG AACTTCGAGG ACGGCGGCGT GGTGACCGTG ACCCAGGACT 421 CCTCCCTGCA GGACGGCGAG TTCATCTACA AGGTGAAGCT GCGCGGCACC AACTTCCCCT 481 CCGACGGCCC CGTAATGCAG TGTCGTACCA TGGGCTGGGA GGCCTCCACT GAGCGGATGT 541 ACCCCGAGGA CGGCGCCCTG AAGGGCGAGA TCAAGCAGAG GCTGAAGCTG AAGGACGGCG 601 GCCACTACGA CGCTGAGGTC AAGACCACCT ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG 661 GCGCCTACAA CGTCGACATC AAGTTGGACA TCCTTTCCCA CAACGAGGAC TACACCATCG 721 TGGAACAGTA CGAACGCGCC GAGGGCCGCC ACTCCACCGG CGGCATGGAC GAGCTGTACA 781 AGTAATCTAG ATAACTGATC ATAATCAGCC ATACCACATT TGTAGAGGTT TTACTTGCTT 841 TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG 901 TTAACTTGTT TATTGCAGCT TATAATGGGT TACAAATAAA GCAATAGCAT CACAAATTTC 961 ACAAATAAAA GCATTTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 1021 TATCTTAACG CGTCGATCAT ATTCAATAAA CCCTTTAATA TTACTTCGTA TAATGTATGC 1081 TATACGAAGT ATTAAGTCTG GAAGAAGGAG TTTACGTTCC AGCCAAGCTA GAATCCTCGG 1141 ACCTCCGAAA TCCTACCGGC TAAGGGAAGG CCGCTTTTTA CA //
Primer: PGKpro_F	RDB13925_A6B8b_2.seq
>D03070A09_A6B8_1_PGK-proF_D07_10.ab1 1 ATCGGGCGTA CTCTCTTCTC ATCTCCGGGC CTTTCGACCT GCATCCATCT AGATCTCGAG 61 CAGCTGAAGC TTACCGCTAG CATGAAAACA TTTAACATTT CTCAACAAGA TCTAGAATTA 121 GTAGAAGTAG CGACAGAGAA GATTACAATG CTTTATGAGG ATAATAAACA TCATGTGGGA 181 GCGGCAATTC GTACGAAAAC AGGAGAAATC ATTTCGGCAG TACATATTGA AGCGTATATA 241 GGACGAGTAA CTGTTTGTGC AGAAGCCATT GCGATTGGTA GTGCAGTTTC GAATGGACAA 301 AAGGATTTTG ACACGATTGT AGCTGTTAGA CACCCTTATT CTGACGAAGT AGATAGAAGT 361 ATTCGAGTGG TAAGTCCTTG TGGTATGTGT AGGGAGTTGA TTTCAGACTA TGCACCAGAT 421 TGTTTTGTGT TAATAGAAAT GAATGGCAAG TTAGTCAAAA CTACGATTGA AGAACTCATT 481 CCACTCAAAT ATACCCGAAA TTAAGCTAGC CGCCCGCCCC ACGACCCGCA GCGCCCGACC 541 GAAAGGAGCG CACGACCCCA TGCATCGATG ATATCAGATC CCCGGGATGC AGAAATTGAT 601 GATCTATTAA ACAATAAAGA TGTCCACTAA AATGGAAGTT TTTCCTGTCA TACTTTGTTA 661 AGAAGGGTGA GAACAGAGTA CCTACATTTT GAATGGAAGG ATTGGAGCTA CGGGGGTGGG 721 GGTGGGGTGG GATTAGATAA ATGCCTGCTC TTTACTGAAG GCTCTTTACT ATTGCTTTAT 781 GATAATGTTT CATAGTTGGA TATCATAATT TAAACAAGCA AAACCAAATT AAGGGCCAGC 841 TCATTCCTCC CACTCATGAT CTATAGATCT ATAGATCTCT CGTGGGATCA TTGTTTTTTC 901 TCTTGATTCC CACTTTGTGG TTCTAAGTAC TGTGGTTTCC AAATGTGTCA GTTTCATAGC 961 CCTGAAGAAC GAGATCAGCA GCCTCTGTTC CACATACACT TCATTCTCAG TATTGTTTTG 1021 CCAAGGTTCT AATTTCCAAT CAGAAGCTGG GTCGAGATCC GGAACCCTTT AAATAATACT 1081 TCGTATATGG TATGCTAATA CGAAAGTAAT AGGTTCCCTT CGAAGAGGTC ACTTAGAATT 1141 CCAGGTAACA GCTTGATCCC CATTGATGAA AGAGT //
Primer: PGK_N_rev	RDB13925_A6B8c_2.seq
>D03070A09_A6B8_1_PGK-N-rev_D06_12.ab1 1 CCTGACGCTC CCTACCGGTA GATTTCGAGG TCGAGATCCT AAGCTTGGCT GGACGTAAAC 61 TCCTCTTCAG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA TTAAGGGTTA 121 TTGAATATGA TCGACGCGTT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG 181 CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT 241 ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG 301 GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TATGGCTGAT 361 TATGATCAGT TATCTAGATT ACTTGTACAG CTCGTCCATG CCGCCGGTGG AGTGGCGGCC 421 CTCGGCGCGT TCGTACTGTT CCACGATGGT GTAGTCCTCG TTGTGGGAAA GGATGTCCAA 481 CTTGATGTCG ACGTTGTAGG CGCCGGGCAG CTGCACGGGC TTCTTGGCCT TGTAGGTGGT 541 CTTGACCTCA GCGTCGTAGT GGCCGCCGTC CTTCAGCTTC AGCCTCTGCT TGATCTCGCC 601 CTTCAGGGCG CCGTCCTCGG GGTACATCCG CTCAGTGGAG GCCTCCCAGC CCATGGTACG 661 ACACTGCATT ACGGGGCCGT CGGAGGGGAA GTTGGTGCCG CGCAGCTTCA CCTTGTAGAT 721 GAACTCGCCG TCCTGCAGGG AGGAGTCCTG GGTCACGGTC ACCACGCCGC CGTCCTCGAA 781 GTTCATCACG CGCTCCCAAT TGAAGCCCTC GGGGAAGGAC AGCTTCAAGT AGTCGGGGAT 841 GTCGGCGGGG TGCTTCACGT AGGCCTTGGA GCCGTACATG AACTGAGGGG ACAGGATGTC 901 CCAGGCGAAG GGCAGGGGGC CACCCTTGGT CACCTTCAGC TTGGCGGTCT GGGTGCCCTC 961 GTAGGGGCGG GCCCTCGCCC TCGCCCTCGA TCTCGAACTC GTGGCCGTTC ACGGAGCCCT 1021 CCATGTGCAC CTTTGAAAGC GCATGACTCC TTGATGATTG GCATGATATC CCTCTTCGCC 1081 TGCTCACAAT GCTAGCCGGC GCTGCACGGA ATCCGAGCTC AATCGGCCTA TTAAGGGTGA 1141 AGTTCGTTAT ATACGCGCGG C //
Primer: M13R	RDB13925_A6B8d_2.seq
>D03070A09_A6B8_1_M13R_F01_16.ab1 1 ATTCGCGGCA TTAACCCTCA CTAAAGGGAA CAAAAGCTGG GTACCGGATC CTAGTGAACC 61 TCTTCGAGGG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA TTAAGGGTTC 121 CGGATCTCGA CCAGCTTCTG ATGGAATTAG AACTTGGCAA AACAATACTG AGAATGAAGT 181 GTATGTGGAA CAGAGGCTGC TGATCTCGTT CTTCAGGCTA TGAAACTGAC ACATTTGGAA 241 ACCACAGTAC TTAGAACCAC AAAGTGGGAA TCAAGAGAAA AACAATGATC CCACGAGAGA 301 TCTATAGATC TATAGATCAT GAGTGGGAGG AATGAGCTGG CCCTTAATTT GGTTTTGCTT 361 GTTTAAATTA TGATATCCAA CTATGAAACA TTATCATAAA GCAATAGTAA AGAGCCTTCA 421 GTAAAGAGCA GGCATTTATC TAATCCCACC CCACCCCCAC CCCCGTAGCT CCAATCCTTC 481 CATTCAAAAT GTAGGTACTC TGTTCTCACC CTTCTTAACA AAGTATGACA GGAAAAACTT 541 CCATTTTAGT GGACATCTTT ATTGTTTAAT AGATCATCAA TTTCTGCATC CCGGGGATCT 601 GATATCATCG ATGCATGGGG TCGTGCGCTC CTTTCGGTCG GGCGCTGCGG GTCGTGGGGC 661 GGGCGGCTAG CTTAATTTCG GGTATATTTG AGTGGAATGA GTTCTTCAAT CGTAGTTTTG 721 ACTAACTTGC CATTCATTTC TATTAACACA AAACAATCTG GTGCATAGTC TGAAAATCAA 781 CTCCCCTACA CATACCACAA GGGACTTACC CAACTCGAAT ACCTTCTATC TACCTTCGTC 841 AGATTAAGGG TGTCCTTAAC AGCCTACCAA TCGTTGTCAA AATCCCTTTT GTCCATTTCG 901 AAAACTTGCC ACTACCAATC GCAATGGCTT CCTGCCAAAA CCAGTTACTC GTCCCAATAT 961 ACGCTTCAAT ATGTACCTGC CGAAATGATT TCCCCCTGTT TTTCGTAGAA ATTGGCCCGC 1021 TCCCCACCTG GAT //

Please visit Sequencing and PCR primers for primer information.

References

original	Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

2018.09.03

GNP_filter3_RDBDEP_html_180823.pl