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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK282 (mCherry2-Bsr)

A plasmid for construction of C-terminal mCherry tagging donors.

Catalog number RDB13925
Resource name pMK282 (mCherry2-Bsr)
Alternative name mCherry2-Bsr, 974
Clone info. A plasmid for construction of C-terminal mCherry tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone.
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Gene/insert name Discosoma sp. RFP cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank

External Database
Discosoma sp. RFP

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
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            RDB13925 pMK282 (mCherry2-Bsr) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pMK282 (mCherry2-Bsr) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13925).

            Reference section:

            Natsume, T., Kiyomitsu, T., Saga, Y., Kanemaki, M.T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


            Tips

            Featured content

            Featured content Auxin Inducible Degron (AID) System (English text)
            Featured content Auxin Inducible Degron (AID) System (Japanese text)
            Featured content Genome Editing (English text)
            Featured content Genome Editing (Japanese text)
            Featured content Bacteria host strains (en) (English text)
            Featured content Bacteria host strains (ja) (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13925_A6B8p1-4.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: M13_-40 (Pr0410)
            Region: T7 pro,insert 5'(Linker,mCherry2 5')
            Sequence file: RDB13925_A6B8e.seq check
            >D03070A09_A6B8_1_M13(-40)_F02_17.ab1
                1 NNNNNNNNNN NNNNNNCNNA NNNGNNTNNC NNTAGGGCGA ATTGGAGCTC GGATCCGGTG
               61 CAGGCGCCGC TAGCATGGTG AGCAAGGGCG AGGAGGATAA CATGGCCATC ATCAAGGAGT
              121 TCATGCGCTT CAAGGTGCAC ATGGAGGGCT CCGTGAACGG CCACGAGTTC GAGATCGAGG
              181 GCGAGGGCGA GGGCCGCCCC TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG
              241 GTGGCCCCCT GCCCTTCGCC TGGGACATCC TGTCCCCTCA GTTCATGTAC GGCTCCAAGG
              301 CCTACGTGAA GCACCCCGCC GACATCCCCG ACTACTTGAA GCTGTCCTTC CCCGAGGGCT
              361 TCAATTGGGA GCGCGTGATG AACTTCGAGG ACGGCGGCGT GGTGACCGTG ACCCAGGACT
              421 CCTCCCTGCA GGACGGCGAG TTCATCTACA AGGTGAAGCT GCGCGGCACC AACTTCCCCT
              481 CCGACGGCCC CGTAATGCAG TGTCGTACCA TGGGCTGGGA GGCCTCCACT GAGCGGATGT
              541 ACCCCGAGGA CGGCGCCCTG AAGGGCGAGA TCAAGCAGAG GCTGAAGCTG AAGGACGGCG
              601 GCCACTACGA CGCTGAGGTC AAGACCACCT ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG
              661 GCGCCTACAA CGTCGACATC AAGTTGGACA TCCTTTCCCA CAACGAGGAC TACACCATCG
              721 TGGAACAGTA CGAACGCGCC GAGGGCCGCC ACTCCACCGG CGGCATGGAC GAGCTGTACA
              781 AGTAATCTAG ATAACTGATC ATAATCAGCC ATACCACATT TGTAGANGNT TTACTTGCTT
              841 NNAAAANNCT CCCACACCTN CCCCTGAACC TGAANNATAA AATGAATGCN ATTGTTGTTG
              901 NTAANTTGTT TATTGCANCT TATAANNGNT TACAAANNAA GCAATAGCAT CNCAAATTTC
              961 ACAAANNAAN NCATTTTTTT TNNCTGNNTT CNANNNNNGG TTTGNCCAAN NNNNNCNNNG
            1021 NNNNNNNNNN
            //
            Primer: M13R (Pr0160)
            Region: insert 3'(loxP,mPGK ter)
            Sequence file: RDB13925_A6B8f.seq check
            >D03070A09_A6B8_1_M13R_F01_16.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNGGNA NNAAAGCTGG GTACCGGATC CTAGTGAACC
               61 TCTTCGAGGG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA TTAAGGGTTC
              121 CGGATCTCGA CCAGCTTCTG ATGGAATTAG AACTTGGCAA AACAATACTG AGAATGAAGT
              181 GTATGTGGAA CAGAGGCTGC TGATCTCGTT CTTCAGGCTA TGAAACTGAC ACATTTGGAA
              241 ACCACAGTAC TTAGAACCAC AAAGTGGGAA TCAAGAGAAA AACAATGATC CCACGAGAGA
              301 TCTATAGATC TATAGATCAT GAGTGGGAGG AATGAGCTGG CCCTTAATTT GGTTTTGCTT
              361 GTTTAAATTA TGATATCCAA CTATGAAACA TTATCATAAA GCAATAGTAA AGAGCCTTCA
              421 GTAAAGAGCA GGCATTTATC TAATCCCACC CCACCCCCAC CCCCGTAGCT CCAATCCTTC
              481 CATTCAAAAT GTAGGTACTC TGTTCTCACC CTTCTTAACA AAGTATGACA GGAAAAACTT
              541 CCATTTTAGT GGACATCTTT ATTGTTTAAT AGATCATCAA TTTCTGCATC CCGGGGATCT
              601 GATATCATCG ATGCATGGGG TCGTGCGCTC CTTTCGGTCG GGCGCTGCGG GTCGTGGGGC
              661 GGGCGGCTAG CTTAATTTCG GGTANNNNNG ANTGGAATGA GTTCTTCAAT CNTAGTTTTG
              721 ACTANNTTGC CATTCATTTC TATTAACACA NNNNNNNCTG GTGCATAGTC TGAAANTCAA
              781 CNNCCCTACA CNTACCACAA NNGACTTNNC CNNCTCGAAT NNNNTCTATC NACNTTCGTC
              841 NNANNAANGN TGNNNNNAAN ANNCTANNAN TCGNNGNNNN ANTNCNTTTT GNCCANTNCN
            901 AAAACNNGNN NNNNNNNN
            //
            Primer: PGK_N_rev (Pr0243)
            Region: insert mid(loxP,SV40pA,mCherry2 3')
            Sequence file: RDB13925_A6B8g.seq check
            >D03070A09_A6B8_1_PGK-N-rev_D06_12.ab1
                1 NNNNNNNNNN NNNNNNNGNN NANTTCGAGG TCGAGNTCCT AAGCTTGGCT GGACGTAAAC
               61 TCCTCTTCAG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA TTAAGGGTTA
              121 TTGAATATGA TCGACGCGTT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG
              181 CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT
              241 ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG
              301 GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TATGGCTGAT
              361 TATGATCAGT TATCTAGATT ACTTGTACAG CTCGTCCATG CCGCCGGTGG AGTGGCGGCC
              421 CTCGGCGCGT TCGTACTGTT CCACGATGGT GTAGTCCTCG TTGTGGGAAA GGATGTCCAA
              481 CTTGATGTCG ACGTTGTAGG CGCCGGGCAG CTGCACGGGC TTCTTGGCCT TGTAGGTGGT
              541 CTTGACCTCA GCGTCGTAGT GGCCGCCGTC CTTCAGCTTC AGCCTCTGCT TGATCTCGCC
              601 CTTCAGGGCG CCGTCCTCGG GGTACATCCG CTCAGTGGAG GCCTCCCAGC CCATGGTACG
              661 ACACTGCATT ACGGGGCCGT CGGANGGGAA GTTGGTGCCG CGCAGCTTCA CCTTGTAGAT
              721 GAACTCGCCG TCCTGCAGGG AGGAGTCCTG GGTCACGGTC ACCACGCCGC CGTCCTCGAA
              781 NTTCATCACG CGCTCNCNAT TGAAGCCCTC NGGGAANGAC AGCTTCAAGT AGTCNGGGAN
              841 GTCNGCGGGG TGCTTCACGT NNNCCTTGGA NCCGTACATG ANNTGANGGG ANANGNNGTN
              901 CCANGCGANN GGNNNGGGGC NACCCTNNGT CACCNNCANC TTNNNNGTCT GGGNGNCCNN
            961 GNNNGGNCNG NNCNNCNNNN NCNNCCNNNN NNNNNN
            //
            Primer: PGKpro_F (Pr0249)
            Region: insert mid(bsr,mPGK ter)
            Sequence file: RDB13925_A6B8h.seq check
            >D03070A09_A6B8_1_PGK-proF_D07_10.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNTCCNNGC CTTTCGACCT GCATCCNTCT AGATCTCGAG
               61 CAGCTGAAGC TTACCGCTAG CATGAAAACA TTTAACATTT CTCAACAAGA TCTAGAATTA
              121 GTAGAAGTAG CGACAGAGAA GATTACAATG CTTTATGAGG ATAATAAACA TCATGTGGGA
              181 GCGGCAATTC GTACGAAAAC AGGAGAAATC ATTTCGGCAG TACATATTGA AGCGTATATA
              241 GGACGAGTAA CTGTTTGTGC AGAAGCCATT GCGATTGGTA GTGCAGTTTC GAATGGACAA
              301 AAGGATTTTG ACACGATTGT AGCTGTTAGA CACCCTTATT CTGACGAAGT AGATAGAAGT
              361 ATTCGAGTGG TAAGTCCTTG TGGTATGTGT AGGGAGTTGA TTTCAGACTA TGCACCAGAT
              421 TGTTTTGTGT TAATAGAAAT GAATGGCAAG TTAGTCAAAA CTACGATTGA AGAACTCATT
              481 CCACTCAAAT ATACCCGAAA TTAAGCTAGC CGCCCGCCCC ACGACCCGCA GCGCCCGACC
              541 GAAAGGAGCG CACGACCCCA TGCATCGATG ATATCAGATC CCCGGGATGC AGAAATTGAT
              601 GATCTATTAA ACAATAAAGA TGTCCACTAA AATGGAAGTT TTTCCTGTCA TACTTTGTTA
              661 AGAAGGGTGA GAACAGAGTA CCTACATTTT GAATGGAAGG ANTGGAGCTA CGGGGGTGGG
              721 GGTGGGGTGG GATTAGATAA ATGCCTGCTC TTTACTGAAG GNTCTTTACT ATTGCTTTAT
              781 GATAATGTTT CATAGTTGGA TATCATAATT TNAACAAGCA AAACCNANTT AANGGNCAGC
              841 TCATTNCTCC CACTCATGAT CNATAGATCT ATAGATCTCT CGNGGGATCA TNGNTTTTTC
              901 TCTTGANTCC CACTTTGTGG TTCNAAGTAC TGTGGTTTCC AAATGTGTCA GTTTCATAGN
            961 CNTGNAGAAN GAGATNANNA GCCNNNNNTN NNNNTANNNT TCNNNNNCAN NNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP]

            2024.03.26

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