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pMK282 (mCherry2-Bsr)

A plasmid for construction of C-terminal mCherry tagging donors.

Catalog number RDB13925
Resource name pMK282 (mCherry2-Bsr)
Alternative name mCherry2-Bsr, 974
Clone info. A plasmid for construction of C-terminal mCherry tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone.
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Gene/insert name Discosoma sp. RFP cDNA
Depositor Kanemaki, Masato |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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Remarks An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13925 pMK282 (mCherry2-Bsr) DNA solution

cDNA Request [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)
Featured content Genome Editing (English text)
Featured content Genome Editing (Japanese text)
Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

References

original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13925_A6B8p1_v2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 RDB13925_A6B8a_2.seq
>D03070A09_A6B8_1_M13(-40)_F02_17.ab1
    1 CCGCCAGCTA GCGGCGCGTA TACGACTCAC TATAGGGCGA ATTGGAGCTC GGATCCGGTG
   61 CAGGCGCCGC TAGCATGGTG AGCAAGGGCG AGGAGGATAA CATGGCCATC ATCAAGGAGT
  121 TCATGCGCTT CAAGGTGCAC ATGGAGGGCT CCGTGAACGG CCACGAGTTC GAGATCGAGG
  181 GCGAGGGCGA GGGCCGCCCC TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG
  241 GTGGCCCCCT GCCCTTCGCC TGGGACATCC TGTCCCCTCA GTTCATGTAC GGCTCCAAGG
  301 CCTACGTGAA GCACCCCGCC GACATCCCCG ACTACTTGAA GCTGTCCTTC CCCGAGGGCT
  361 TCAATTGGGA GCGCGTGATG AACTTCGAGG ACGGCGGCGT GGTGACCGTG ACCCAGGACT
  421 CCTCCCTGCA GGACGGCGAG TTCATCTACA AGGTGAAGCT GCGCGGCACC AACTTCCCCT
  481 CCGACGGCCC CGTAATGCAG TGTCGTACCA TGGGCTGGGA GGCCTCCACT GAGCGGATGT
  541 ACCCCGAGGA CGGCGCCCTG AAGGGCGAGA TCAAGCAGAG GCTGAAGCTG AAGGACGGCG
  601 GCCACTACGA CGCTGAGGTC AAGACCACCT ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG
  661 GCGCCTACAA CGTCGACATC AAGTTGGACA TCCTTTCCCA CAACGAGGAC TACACCATCG
  721 TGGAACAGTA CGAACGCGCC GAGGGCCGCC ACTCCACCGG CGGCATGGAC GAGCTGTACA
  781 AGTAATCTAG ATAACTGATC ATAATCAGCC ATACCACATT TGTAGAGGTT TTACTTGCTT
  841 TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG
  901 TTAACTTGTT TATTGCAGCT TATAATGGGT TACAAATAAA GCAATAGCAT CACAAATTTC
  961 ACAAATAAAA GCATTTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG
 1021 TATCTTAACG CGTCGATCAT ATTCAATAAA CCCTTTAATA TTACTTCGTA TAATGTATGC
 1081 TATACGAAGT ATTAAGTCTG GAAGAAGGAG TTTACGTTCC AGCCAAGCTA GAATCCTCGG
1141 ACCTCCGAAA TCCTACCGGC TAAGGGAAGG CCGCTTTTTA CA
//
Primer: PGKpro_F RDB13925_A6B8b_2.seq
>D03070A09_A6B8_1_PGK-proF_D07_10.ab1
    1 ATCGGGCGTA CTCTCTTCTC ATCTCCGGGC CTTTCGACCT GCATCCATCT AGATCTCGAG
   61 CAGCTGAAGC TTACCGCTAG CATGAAAACA TTTAACATTT CTCAACAAGA TCTAGAATTA
  121 GTAGAAGTAG CGACAGAGAA GATTACAATG CTTTATGAGG ATAATAAACA TCATGTGGGA
  181 GCGGCAATTC GTACGAAAAC AGGAGAAATC ATTTCGGCAG TACATATTGA AGCGTATATA
  241 GGACGAGTAA CTGTTTGTGC AGAAGCCATT GCGATTGGTA GTGCAGTTTC GAATGGACAA
  301 AAGGATTTTG ACACGATTGT AGCTGTTAGA CACCCTTATT CTGACGAAGT AGATAGAAGT
  361 ATTCGAGTGG TAAGTCCTTG TGGTATGTGT AGGGAGTTGA TTTCAGACTA TGCACCAGAT
  421 TGTTTTGTGT TAATAGAAAT GAATGGCAAG TTAGTCAAAA CTACGATTGA AGAACTCATT
  481 CCACTCAAAT ATACCCGAAA TTAAGCTAGC CGCCCGCCCC ACGACCCGCA GCGCCCGACC
  541 GAAAGGAGCG CACGACCCCA TGCATCGATG ATATCAGATC CCCGGGATGC AGAAATTGAT
  601 GATCTATTAA ACAATAAAGA TGTCCACTAA AATGGAAGTT TTTCCTGTCA TACTTTGTTA
  661 AGAAGGGTGA GAACAGAGTA CCTACATTTT GAATGGAAGG ATTGGAGCTA CGGGGGTGGG
  721 GGTGGGGTGG GATTAGATAA ATGCCTGCTC TTTACTGAAG GCTCTTTACT ATTGCTTTAT
  781 GATAATGTTT CATAGTTGGA TATCATAATT TAAACAAGCA AAACCAAATT AAGGGCCAGC
  841 TCATTCCTCC CACTCATGAT CTATAGATCT ATAGATCTCT CGTGGGATCA TTGTTTTTTC
  901 TCTTGATTCC CACTTTGTGG TTCTAAGTAC TGTGGTTTCC AAATGTGTCA GTTTCATAGC
  961 CCTGAAGAAC GAGATCAGCA GCCTCTGTTC CACATACACT TCATTCTCAG TATTGTTTTG
 1021 CCAAGGTTCT AATTTCCAAT CAGAAGCTGG GTCGAGATCC GGAACCCTTT AAATAATACT
 1081 TCGTATATGG TATGCTAATA CGAAAGTAAT AGGTTCCCTT CGAAGAGGTC ACTTAGAATT
1141 CCAGGTAACA GCTTGATCCC CATTGATGAA AGAGT
//
Primer: PGK_N_rev RDB13925_A6B8c_2.seq
>D03070A09_A6B8_1_PGK-N-rev_D06_12.ab1
    1 CCTGACGCTC CCTACCGGTA GATTTCGAGG TCGAGATCCT AAGCTTGGCT GGACGTAAAC
   61 TCCTCTTCAG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA TTAAGGGTTA
  121 TTGAATATGA TCGACGCGTT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG
  181 CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT
  241 ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG
  301 GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TATGGCTGAT
  361 TATGATCAGT TATCTAGATT ACTTGTACAG CTCGTCCATG CCGCCGGTGG AGTGGCGGCC
  421 CTCGGCGCGT TCGTACTGTT CCACGATGGT GTAGTCCTCG TTGTGGGAAA GGATGTCCAA
  481 CTTGATGTCG ACGTTGTAGG CGCCGGGCAG CTGCACGGGC TTCTTGGCCT TGTAGGTGGT
  541 CTTGACCTCA GCGTCGTAGT GGCCGCCGTC CTTCAGCTTC AGCCTCTGCT TGATCTCGCC
  601 CTTCAGGGCG CCGTCCTCGG GGTACATCCG CTCAGTGGAG GCCTCCCAGC CCATGGTACG
  661 ACACTGCATT ACGGGGCCGT CGGAGGGGAA GTTGGTGCCG CGCAGCTTCA CCTTGTAGAT
  721 GAACTCGCCG TCCTGCAGGG AGGAGTCCTG GGTCACGGTC ACCACGCCGC CGTCCTCGAA
  781 GTTCATCACG CGCTCCCAAT TGAAGCCCTC GGGGAAGGAC AGCTTCAAGT AGTCGGGGAT
  841 GTCGGCGGGG TGCTTCACGT AGGCCTTGGA GCCGTACATG AACTGAGGGG ACAGGATGTC
  901 CCAGGCGAAG GGCAGGGGGC CACCCTTGGT CACCTTCAGC TTGGCGGTCT GGGTGCCCTC
  961 GTAGGGGCGG GCCCTCGCCC TCGCCCTCGA TCTCGAACTC GTGGCCGTTC ACGGAGCCCT
 1021 CCATGTGCAC CTTTGAAAGC GCATGACTCC TTGATGATTG GCATGATATC CCTCTTCGCC
 1081 TGCTCACAAT GCTAGCCGGC GCTGCACGGA ATCCGAGCTC AATCGGCCTA TTAAGGGTGA
1141 AGTTCGTTAT ATACGCGCGG C
//
Primer: M13R RDB13925_A6B8d_2.seq
>D03070A09_A6B8_1_M13R_F01_16.ab1
    1 ATTCGCGGCA TTAACCCTCA CTAAAGGGAA CAAAAGCTGG GTACCGGATC CTAGTGAACC
   61 TCTTCGAGGG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA TTAAGGGTTC
  121 CGGATCTCGA CCAGCTTCTG ATGGAATTAG AACTTGGCAA AACAATACTG AGAATGAAGT
  181 GTATGTGGAA CAGAGGCTGC TGATCTCGTT CTTCAGGCTA TGAAACTGAC ACATTTGGAA
  241 ACCACAGTAC TTAGAACCAC AAAGTGGGAA TCAAGAGAAA AACAATGATC CCACGAGAGA
  301 TCTATAGATC TATAGATCAT GAGTGGGAGG AATGAGCTGG CCCTTAATTT GGTTTTGCTT
  361 GTTTAAATTA TGATATCCAA CTATGAAACA TTATCATAAA GCAATAGTAA AGAGCCTTCA
  421 GTAAAGAGCA GGCATTTATC TAATCCCACC CCACCCCCAC CCCCGTAGCT CCAATCCTTC
  481 CATTCAAAAT GTAGGTACTC TGTTCTCACC CTTCTTAACA AAGTATGACA GGAAAAACTT
  541 CCATTTTAGT GGACATCTTT ATTGTTTAAT AGATCATCAA TTTCTGCATC CCGGGGATCT
  601 GATATCATCG ATGCATGGGG TCGTGCGCTC CTTTCGGTCG GGCGCTGCGG GTCGTGGGGC
  661 GGGCGGCTAG CTTAATTTCG GGTATATTTG AGTGGAATGA GTTCTTCAAT CGTAGTTTTG
  721 ACTAACTTGC CATTCATTTC TATTAACACA AAACAATCTG GTGCATAGTC TGAAAATCAA
  781 CTCCCCTACA CATACCACAA GGGACTTACC CAACTCGAAT ACCTTCTATC TACCTTCGTC
  841 AGATTAAGGG TGTCCTTAAC AGCCTACCAA TCGTTGTCAA AATCCCTTTT GTCCATTTCG
  901 AAAACTTGCC ACTACCAATC GCAATGGCTT CCTGCCAAAA CCAGTTACTC GTCCCAATAT
  961 ACGCTTCAAT ATGTACCTGC CGAAATGATT TCCCCCTGTT TTTCGTAGAA ATTGGCCCGC
1021 TCCCCACCTG GAT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

2018.09.03

GNP_filter3_RDBDEP_html_180823.pl