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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK376 (Hygro-P2A-mClover)

A plasmid for construction of N-terminal Hygro-P2A-mClover tagging donors.

Catalog number RDB16814
Resource name pMK376 (Hygro-P2A-mClover)
Clone info. A plasmid for construction of N-terminal Hygro-P2A-mClover tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Schedule A (GFP-A) [Link]
            Remarks
            Please use Schedule A (GFP-A) additionally.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            Schedule A(GFP-A, 英語版のみ) [Link]
            備考
            Schedule A(GFP-A, 英語版のみ)が追加で必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB16814 pMK376 (Hygro-P2A-mClover) DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Auxin Inducible Degron (AID) System (English text)
            Featured content Auxin Inducible Degron (AID) System (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB16814_A8Lpp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pUC-M13_-100
            Region: T7 pro,insert 5'(Hyg R 5')
            Sequence file: RDB16814_A8Lpd.seq check
            >D06157B1_A8LP_2_pUC-M13_-100_C04_07_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNT GGGNANGCNN GGGNTTTCCC NGTCNCGACN TTGTAAAACG
               61 ACGGCCAGTG NGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGGTAC CGGGCCCCCC
              121 CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGCCACCA TGAAAAAGCC TGAACTCACC
              181 GCGACGTCTG TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTCTCCGA CCTGATGCAG
              241 CTCTCGGAGG GCGAAGAATC TCGTGCTTTC AGCTTCGATG TAGGAGGGCG TGGATATGTC
              301 CTGCGGGTAA ATAGCTGCGC CGATGGTTTC TACAAAGATC GTTATGTTTA TCGGCACTTT
              361 GCATCGGCCG CGCTCCCGAT TCCGGAAGTG CTTGACATTG GGGAATTCAG CGAGAGCCTG
              421 ACCTATTGCA TCTCCCGCCG TGCACAGGGT GTCACGTTGC AAGACCTGCC TGAAACCGAA
              481 CTGCCCGCTG TTCTGCAGCC GGTCGCGGAG GCCATGGATG CGATCGCTGC GGCCGATCTT
              541 AGCCAGACGA GCGGGTTCGG CCCATTCGGA CCGCAAGGAA TCGGTCAATA CACTACATGG
              601 CGTGATTTCA TATGCGCGAT TGCTGATCCC CATGTGTATC ACTGGCAAAC TGTGATGGAC
              661 GACACCGTCA GTGCGTCCGT CGCGCANGCT CTCGATGANC TGATGCTTTG GGCCGAGGAC
              721 TGCCCCGAAG TCCGGCACCT CGTGCACGCG GATTTCGGCT CCAACAATGT CCTGACGGNC
              781 AATGGCCGCA TAACAGCGGT CATTGACTGG ANCGAGGCGA TGTTCGGGGN TTCCCAATAC
              841 GANGTCGCNN ACNTCTTCTT CTGGANGNNG NGGTTGGCTN GTATNGANCA NCANNNGCGC
              901 TACTTCNANN NNNNNNNTNN NNNNCTTNNN NNNNCNNNNN NGNNNNNGGN NNANNNNNNN
            961 NNN
            //
            Primer: reverse
            Region: Linker,insert 3'(mClover 3'(A206K))
            Sequence file: RDB16814_A8Lpe.seq check
            >D06157B1_A8LP_2_reverse_D04_10_ABI24.ab1
                1 NNNNNNNNNN NNNNNCNNNA ANGGGNNNNN NNGCTGGAGC TCCNCCGCGG TGGCGGCCGC
               61 TCTAGAACTA GTGGATCCGG CGCCTGCACC AGCGCCCTTG TACAGCTCGT CCATGCCATG
              121 TGTAATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG
              181 GTCTTTGCTC AGCTTGGACT GATGGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC
              241 GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCTCAAC
              301 GTTGTGGCGG ATCTTGAAGT TAGCCTTGAT GCCGTTCTTC TGCTTGTCGG CCGTGATATA
              361 GACGTTGTGG CTGTTGAAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT
              421 GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC
              481 GGCGCGGGTC TTGTAGGTAC CGTCGTCCTT GAAAGAGATG GTGCGCTCCT GGACGTAGCC
              541 TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGCTGAA
              601 GCAGGCCACG CCGTANCCGA AGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC
              661 GGTGGTGCAG ATGAACTTCA GGGTCAGCTT GCCGTTGGTG GCATCGCCCT CGCCCTCGCC
              721 GCGGANGCTG AACTTGTGGC CGTTTACGTC GCCGTCCAGC TCGACCAGGA TGGGCACCAC
              781 CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC CATAGATCTA GGNCCAGGGT TCTCCTCCAC
              841 NNCTNNNGCC TGCTTCAGCA NGCTGAAGTT AGTAGCTCCG CTNNCNGATT CCTTNGNCNN
              901 CNGANNANNG CTGGGGGCNN CNGNNTTNCN CTATCNNNNA NNANTTCTAC NNANNNNTCG
            961 NNNCNNANNN NNNNNNN
            //
            Primer: HygR-C_F
            Region: insert mid(Hyg R 3',P2A,mClover 5')
            Sequence file: RDB16814_A8Lpf.seq check
            >D06157B1_A8LP_2_HygR-C_F_E04_13_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN GNNGTCGCCN ACNTCTTCTT CTGGANGCCG TGGTTGGCTT
               61 GTATGGAGCA GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCGC
              121 GGCTCCGGGC GTATATGCTC CGCATTGGTC TTGACCAACT CTATCAGAGC TTGGTTGACG
              181 GCAATTTCGA TGATGCAGCT TGGGCGCAGG GTCGATGCGA CGCAATCGTC CGATCCGGAG
              241 CCGGGACTGT CGGGCGTACA CAAATCGCCC GCAGAAGCGC GGCCGTCTGG ACCGATGGCT
              301 GTGTAGAAGT ACTCGCCGAT AGTGGAAACC GACGCCCCAG CACTCGTCCG AGGGCAAAGG
              361 AATCAGGAAG CGGAGCTACT AACTTCAGCC TGCTGAAGCA GGCTGGAGAC GTGGAGGAGA
              421 ACCCTGGACC TAGATCTATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA
              481 TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTCCGC GGCGAGGGCG
              541 AGGGCGATGC CACCAACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC
              601 CCGTGCCCTG GCCCACCCTC GTGACCACCT TCGGCTACGG CGTGGCCTGC TTCAGCCGCT
              661 ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA NGCTACGTCC
              721 AGGAGCGCAC CATCTCTTTC AAGGACGACN GTACCTACAA GACCCGCGCC GANGTGAAGT
              781 TCGAGGGCGA NACCCTGGTG AACCGCATCG AGCTGAANGG CATCGACTTC AANGAGGANG
              841 GCAACATCCN GGGGGCACAN NCTGGANTAC NACNTNNACA GCCNCAACGN CTATATCANN
            901 GNCGANNANN AGAANNANCG GNNTCNANNN NTNNNNNNNN NN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

            2022.02.04

            GNP_filter3_RDBDEP_html_220112.pl