RDB16814 - pMK376 (Hygro-P2A-mClover)

Resource data sheet
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pMK376 (Hygro-P2A-mClover)

A plasmid for construction of N-terminal Hygro-P2A-mClover tagging donors.

Catalog number	RDB16814
Resource name	pMK376 (Hygro-P2A-mClover)
Clone info.	A plasmid for construction of N-terminal Hygro-P2A-mClover tagging donors.
Vector backbone	pBluescript (plasmid)
Size of vector backbone	2.9 kb
Selectable markers	Am^r (E. coli)
Gene/insert name	Aequorea victoria GFP cDNA
Depositor	Kanemaki, Masato \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

Ordering Please visit Information of Request for Distribution.
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]

Remarks
Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB16814	pMK376 (Hygro-P2A-mClover)	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original	Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

Further references such as user reports and related articles (go to bottom)

Featured content	Auxin Inducible Degron (AID) System (English text)
Featured content	Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB16814_A8Lpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100	RDB16814_A8Lpa.seq
>D06157B1_A8LP_2_pUC-M13_-100_C04_07_ABI24.ab1 1 GAAGGTCAAG CGGATTAGTT GGGTACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG 61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGGTAC CGGGCCCCCC 121 CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGCCACCA TGAAAAAGCC TGAACTCACC 181 GCGACGTCTG TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTCTCCGA CCTGATGCAG 241 CTCTCGGAGG GCGAAGAATC TCGTGCTTTC AGCTTCGATG TAGGAGGGCG TGGATATGTC 301 CTGCGGGTAA ATAGCTGCGC CGATGGTTTC TACAAAGATC GTTATGTTTA TCGGCACTTT 361 GCATCGGCCG CGCTCCCGAT TCCGGAAGTG CTTGACATTG GGGAATTCAG CGAGAGCCTG 421 ACCTATTGCA TCTCCCGCCG TGCACAGGGT GTCACGTTGC AAGACCTGCC TGAAACCGAA 481 CTGCCCGCTG TTCTGCAGCC GGTCGCGGAG GCCATGGATG CGATCGCTGC GGCCGATCTT 541 AGCCAGACGA GCGGGTTCGG CCCATTCGGA CCGCAAGGAA TCGGTCAATA CACTACATGG 601 CGTGATTTCA TATGCGCGAT TGCTGATCCC CATGTGTATC ACTGGCAAAC TGTGATGGAC 661 GACACCGTCA GTGCGTCCGT CGCGCAGGCT CTCGATGAGC TGATGCTTTG GGCCGAGGAC 721 TGCCCCGAAG TCCGGCACCT CGTGCACGCG GATTTCGGCT CCAACAATGT CCTGACGGAC 781 AATGGCCGCA TAACAGCGGT CATTGACTGG AGCGAGGCGA TGTTCGGGGA TTCCCAATAC 841 GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT GTATGGAGCA GCAGACGCGC 901 TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCGC GGCTCCGGGC GTATATGCTC 961 CGCATTGGTC TTTGACCAAC TCTATCAGAG CTTTGGATGA CGGCAATTTT CGAATGATGC 1021 AGCTTTAGCG CACGGTCGAA TGCCGACGCC ATCGTCGATC GGAGCTGGAC TGTCCGTCGT 1081 ACAACAAATC GTCCTCTGAC AGA //
Primer: reverse	RDB16814_A8Lpb.seq
>D06157B1_A8LP_2_reverse_D04_10_ABI24.ab1 1 CTCGAACATT ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCCACCGCGG TGGCGGCCGC 61 TCTAGAACTA GTGGATCCGG CGCCTGCACC AGCGCCCTTG TACAGCTCGT CCATGCCATG 121 TGTAATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG 181 GTCTTTGCTC AGCTTGGACT GATGGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC 241 GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCTCAAC 301 GTTGTGGCGG ATCTTGAAGT TAGCCTTGAT GCCGTTCTTC TGCTTGTCGG CCGTGATATA 361 GACGTTGTGG CTGTTGAAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT 421 GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC 481 GGCGCGGGTC TTGTAGGTAC CGTCGTCCTT GAAAGAGATG GTGCGCTCCT GGACGTAGCC 541 TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGCTGAA 601 GCAGGCCACG CCGTAGCCGA AGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC 661 GGTGGTGCAG ATGAACTTCA GGGTCAGCTT GCCGTTGGTG GCATCGCCCT CGCCCTCGCC 721 GCGGACGCTG AACTTGTGGC CGTTTACGTC GCCGTCCAGC TCGACCAGGA TGGGCACCAC 781 CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC CATAGATCTA GGTCCAGGGT TCTCCTCCAC 841 GTCTCCAGCC TGCTTCAGCA GGCTGAAGTT AGTAGCTCCG CTTCCTGATT CCTTTGCCCT 901 CGGACGAGTG CTGGGGGCGT CGGGTTTCCA CTATCGGCGA GTACTTCTAC ACAGCCATCG 961 GTCCAGACGG CCGCGCTTCT GCGGGCGATT TGTGTACGCC CGACAGTCCC GGCTCCCGGA 1021 TCGGAACGAA TTGCGTCGCA ATCGAACCCT GGCGCCCAGG CTGCCATCAT CGTAATGCCC 1081 GGTCCACTAA GCTCTGGAAT TGAGTGATCG AGATCCTAAG TTGTAACGA //
Primer: HygR-C_F	RDB16814_A8Lpc.seq
>D06157B1_A8LP_2_HygR-C_F_E04_13_ABI24.ab1 1 GAAATCGGGG CATCCAATAC GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT 61 GTATGGAGCA GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCGC 121 GGCTCCGGGC GTATATGCTC CGCATTGGTC TTGACCAACT CTATCAGAGC TTGGTTGACG 181 GCAATTTCGA TGATGCAGCT TGGGCGCAGG GTCGATGCGA CGCAATCGTC CGATCCGGAG 241 CCGGGACTGT CGGGCGTACA CAAATCGCCC GCAGAAGCGC GGCCGTCTGG ACCGATGGCT 301 GTGTAGAAGT ACTCGCCGAT AGTGGAAACC GACGCCCCAG CACTCGTCCG AGGGCAAAGG 361 AATCAGGAAG CGGAGCTACT AACTTCAGCC TGCTGAAGCA GGCTGGAGAC GTGGAGGAGA 421 ACCCTGGACC TAGATCTATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA 481 TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTCCGC GGCGAGGGCG 541 AGGGCGATGC CACCAACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC 601 CCGTGCCCTG GCCCACCCTC GTGACCACCT TCGGCTACGG CGTGGCCTGC TTCAGCCGCT 661 ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC 721 AGGAGCGCAC CATCTCTTTC AAGGACGACG GTACCTACAA GACCCGCGCC GAGGTGAAGT 781 TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG 841 GCAACATCCT GGGGGCACAA GCTGGAGTAC AACTTCAACA GCCACAACGT CTATATCACG 901 GCCGACAAGC AGAAGGAACG GCATCAAAGG CTAACTTCAA GATCCGCCAC AACGTTGAGG 961 ACGGCAGCGT GCAGCTTCGC CCGACCACTA CCCAGCAGAA CACCCCCATC GGCCGACCGA 1021 CCCCGTGCTG CTGGCCCGGA CCACAACTAC TGAGTCATCA GTTCCAGCTG AAGTAAAGAC 1081 CCCCAACCGA GAAAGTCGCG GATTCACT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

2019.07.12

GNP_filter3_RDBDEP_html_190431.pl