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pMK376 (Hygro-P2A-mClover) (#RDB16814)

A plasmid for construction of N-terminal Hygro-P2A-mClover tagging donors.

Clone info. A plasmid for construction of N-terminal Hygro-P2A-mClover tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Growth conditions LB+Amp, 37oC
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Kanemaki, Masato |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16814 pMK376 (Hygro-P2A-mClover) DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMK376 (Hygro-P2A-mClover) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16814).

Reference section:

Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16814_A8Lpp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310)
Region: T7 pro,insert 5'(Hyg R 5')
Sequence file: RDB16814_A8Lpd.seq check
>D06157B1_A8LP_2_pUC-M13_-100_C04_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT GGGNANGCNN GGGNTTTCCC NGTCNCGACN TTGTAAAACG
   61 ACGGCCAGTG NGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGGTAC CGGGCCCCCC
  121 CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGCCACCA TGAAAAAGCC TGAACTCACC
  181 GCGACGTCTG TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTCTCCGA CCTGATGCAG
  241 CTCTCGGAGG GCGAAGAATC TCGTGCTTTC AGCTTCGATG TAGGAGGGCG TGGATATGTC
  301 CTGCGGGTAA ATAGCTGCGC CGATGGTTTC TACAAAGATC GTTATGTTTA TCGGCACTTT
  361 GCATCGGCCG CGCTCCCGAT TCCGGAAGTG CTTGACATTG GGGAATTCAG CGAGAGCCTG
  421 ACCTATTGCA TCTCCCGCCG TGCACAGGGT GTCACGTTGC AAGACCTGCC TGAAACCGAA
  481 CTGCCCGCTG TTCTGCAGCC GGTCGCGGAG GCCATGGATG CGATCGCTGC GGCCGATCTT
  541 AGCCAGACGA GCGGGTTCGG CCCATTCGGA CCGCAAGGAA TCGGTCAATA CACTACATGG
  601 CGTGATTTCA TATGCGCGAT TGCTGATCCC CATGTGTATC ACTGGCAAAC TGTGATGGAC
  661 GACACCGTCA GTGCGTCCGT CGCGCANGCT CTCGATGANC TGATGCTTTG GGCCGAGGAC
  721 TGCCCCGAAG TCCGGCACCT CGTGCACGCG GATTTCGGCT CCAACAATGT CCTGACGGNC
  781 AATGGCCGCA TAACAGCGGT CATTGACTGG ANCGAGGCGA TGTTCGGGGN TTCCCAATAC
  841 GANGTCGCNN ACNTCTTCTT CTGGANGNNG NGGTTGGCTN GTATNGANCA NCANNNGCGC
  901 TACTTCNANN NNNNNNNTNN NNNNCTTNNN NNNNCNNNNN NGNNNNNGGN NNANNNNNNN
961 NNN
//
Primer: reverse (Pr0099)
Region: Linker,insert 3'(mClover 3'(A206K))
Sequence file: RDB16814_A8Lpe.seq check
>D06157B1_A8LP_2_reverse_D04_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNCNNNA ANGGGNNNNN NNGCTGGAGC TCCNCCGCGG TGGCGGCCGC
   61 TCTAGAACTA GTGGATCCGG CGCCTGCACC AGCGCCCTTG TACAGCTCGT CCATGCCATG
  121 TGTAATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG
  181 GTCTTTGCTC AGCTTGGACT GATGGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC
  241 GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCTCAAC
  301 GTTGTGGCGG ATCTTGAAGT TAGCCTTGAT GCCGTTCTTC TGCTTGTCGG CCGTGATATA
  361 GACGTTGTGG CTGTTGAAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT
  421 GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC
  481 GGCGCGGGTC TTGTAGGTAC CGTCGTCCTT GAAAGAGATG GTGCGCTCCT GGACGTAGCC
  541 TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGCTGAA
  601 GCAGGCCACG CCGTANCCGA AGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC
  661 GGTGGTGCAG ATGAACTTCA GGGTCAGCTT GCCGTTGGTG GCATCGCCCT CGCCCTCGCC
  721 GCGGANGCTG AACTTGTGGC CGTTTACGTC GCCGTCCAGC TCGACCAGGA TGGGCACCAC
  781 CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC CATAGATCTA GGNCCAGGGT TCTCCTCCAC
  841 NNCTNNNGCC TGCTTCAGCA NGCTGAAGTT AGTAGCTCCG CTNNCNGATT CCTTNGNCNN
  901 CNGANNANNG CTGGGGGCNN CNGNNTTNCN CTATCNNNNA NNANTTCTAC NNANNNNTCG
961 NNNCNNANNN NNNNNNN
//
Primer: HygR-C_F (Pr0501)
Region: insert mid(Hyg R 3',P2A,mClover 5')
Sequence file: RDB16814_A8Lpf.seq check
>D06157B1_A8LP_2_HygR-C_F_E04_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GNNGTCGCCN ACNTCTTCTT CTGGANGCCG TGGTTGGCTT
   61 GTATGGAGCA GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCGC
  121 GGCTCCGGGC GTATATGCTC CGCATTGGTC TTGACCAACT CTATCAGAGC TTGGTTGACG
  181 GCAATTTCGA TGATGCAGCT TGGGCGCAGG GTCGATGCGA CGCAATCGTC CGATCCGGAG
  241 CCGGGACTGT CGGGCGTACA CAAATCGCCC GCAGAAGCGC GGCCGTCTGG ACCGATGGCT
  301 GTGTAGAAGT ACTCGCCGAT AGTGGAAACC GACGCCCCAG CACTCGTCCG AGGGCAAAGG
  361 AATCAGGAAG CGGAGCTACT AACTTCAGCC TGCTGAAGCA GGCTGGAGAC GTGGAGGAGA
  421 ACCCTGGACC TAGATCTATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA
  481 TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTCCGC GGCGAGGGCG
  541 AGGGCGATGC CACCAACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC
  601 CCGTGCCCTG GCCCACCCTC GTGACCACCT TCGGCTACGG CGTGGCCTGC TTCAGCCGCT
  661 ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA NGCTACGTCC
  721 AGGAGCGCAC CATCTCTTTC AAGGACGACN GTACCTACAA GACCCGCGCC GANGTGAAGT
  781 TCGAGGGCGA NACCCTGGTG AACCGCATCG AGCTGAANGG CATCGACTTC AANGAGGANG
  841 GCAACATCCN GGGGGCACAN NCTGGANTAC NACNTNNACA GCCNCAACGN CTATATCANN
901 GNCGANNANN AGAANNANCG GNNTCNANNN NTNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]