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pMK376 (Hygro-P2A-mClover)

A plasmid for construction of N-terminal Hygro-P2A-mClover tagging donors.

Catalog number RDB16814
Resource name pMK376 (Hygro-P2A-mClover)
Clone info. A plasmid for construction of N-terminal Hygro-P2A-mClover tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Aequorea victoria GFP cDNA
Depositor Kanemaki, Masato |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16814 pMK376 (Hygro-P2A-mClover) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16814_A8Lpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 RDB16814_A8Lpa.seq
>D06157B1_A8LP_2_pUC-M13_-100_C04_07_ABI24.ab1
    1 GAAGGTCAAG CGGATTAGTT GGGTACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG
   61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGGTAC CGGGCCCCCC
  121 CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGCCACCA TGAAAAAGCC TGAACTCACC
  181 GCGACGTCTG TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTCTCCGA CCTGATGCAG
  241 CTCTCGGAGG GCGAAGAATC TCGTGCTTTC AGCTTCGATG TAGGAGGGCG TGGATATGTC
  301 CTGCGGGTAA ATAGCTGCGC CGATGGTTTC TACAAAGATC GTTATGTTTA TCGGCACTTT
  361 GCATCGGCCG CGCTCCCGAT TCCGGAAGTG CTTGACATTG GGGAATTCAG CGAGAGCCTG
  421 ACCTATTGCA TCTCCCGCCG TGCACAGGGT GTCACGTTGC AAGACCTGCC TGAAACCGAA
  481 CTGCCCGCTG TTCTGCAGCC GGTCGCGGAG GCCATGGATG CGATCGCTGC GGCCGATCTT
  541 AGCCAGACGA GCGGGTTCGG CCCATTCGGA CCGCAAGGAA TCGGTCAATA CACTACATGG
  601 CGTGATTTCA TATGCGCGAT TGCTGATCCC CATGTGTATC ACTGGCAAAC TGTGATGGAC
  661 GACACCGTCA GTGCGTCCGT CGCGCAGGCT CTCGATGAGC TGATGCTTTG GGCCGAGGAC
  721 TGCCCCGAAG TCCGGCACCT CGTGCACGCG GATTTCGGCT CCAACAATGT CCTGACGGAC
  781 AATGGCCGCA TAACAGCGGT CATTGACTGG AGCGAGGCGA TGTTCGGGGA TTCCCAATAC
  841 GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT GTATGGAGCA GCAGACGCGC
  901 TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCGC GGCTCCGGGC GTATATGCTC
  961 CGCATTGGTC TTTGACCAAC TCTATCAGAG CTTTGGATGA CGGCAATTTT CGAATGATGC
 1021 AGCTTTAGCG CACGGTCGAA TGCCGACGCC ATCGTCGATC GGAGCTGGAC TGTCCGTCGT
1081 ACAACAAATC GTCCTCTGAC AGA
//
Primer: reverse RDB16814_A8Lpb.seq
>D06157B1_A8LP_2_reverse_D04_10_ABI24.ab1
    1 CTCGAACATT ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCCACCGCGG TGGCGGCCGC
   61 TCTAGAACTA GTGGATCCGG CGCCTGCACC AGCGCCCTTG TACAGCTCGT CCATGCCATG
  121 TGTAATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG
  181 GTCTTTGCTC AGCTTGGACT GATGGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC
  241 GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCTCAAC
  301 GTTGTGGCGG ATCTTGAAGT TAGCCTTGAT GCCGTTCTTC TGCTTGTCGG CCGTGATATA
  361 GACGTTGTGG CTGTTGAAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT
  421 GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC
  481 GGCGCGGGTC TTGTAGGTAC CGTCGTCCTT GAAAGAGATG GTGCGCTCCT GGACGTAGCC
  541 TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGCTGAA
  601 GCAGGCCACG CCGTAGCCGA AGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC
  661 GGTGGTGCAG ATGAACTTCA GGGTCAGCTT GCCGTTGGTG GCATCGCCCT CGCCCTCGCC
  721 GCGGACGCTG AACTTGTGGC CGTTTACGTC GCCGTCCAGC TCGACCAGGA TGGGCACCAC
  781 CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC CATAGATCTA GGTCCAGGGT TCTCCTCCAC
  841 GTCTCCAGCC TGCTTCAGCA GGCTGAAGTT AGTAGCTCCG CTTCCTGATT CCTTTGCCCT
  901 CGGACGAGTG CTGGGGGCGT CGGGTTTCCA CTATCGGCGA GTACTTCTAC ACAGCCATCG
  961 GTCCAGACGG CCGCGCTTCT GCGGGCGATT TGTGTACGCC CGACAGTCCC GGCTCCCGGA
 1021 TCGGAACGAA TTGCGTCGCA ATCGAACCCT GGCGCCCAGG CTGCCATCAT CGTAATGCCC
1081 GGTCCACTAA GCTCTGGAAT TGAGTGATCG AGATCCTAAG TTGTAACGA
//
Primer: HygR-C_F RDB16814_A8Lpc.seq
>D06157B1_A8LP_2_HygR-C_F_E04_13_ABI24.ab1
    1 GAAATCGGGG CATCCAATAC GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT
   61 GTATGGAGCA GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCGC
  121 GGCTCCGGGC GTATATGCTC CGCATTGGTC TTGACCAACT CTATCAGAGC TTGGTTGACG
  181 GCAATTTCGA TGATGCAGCT TGGGCGCAGG GTCGATGCGA CGCAATCGTC CGATCCGGAG
  241 CCGGGACTGT CGGGCGTACA CAAATCGCCC GCAGAAGCGC GGCCGTCTGG ACCGATGGCT
  301 GTGTAGAAGT ACTCGCCGAT AGTGGAAACC GACGCCCCAG CACTCGTCCG AGGGCAAAGG
  361 AATCAGGAAG CGGAGCTACT AACTTCAGCC TGCTGAAGCA GGCTGGAGAC GTGGAGGAGA
  421 ACCCTGGACC TAGATCTATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA
  481 TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTCCGC GGCGAGGGCG
  541 AGGGCGATGC CACCAACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC
  601 CCGTGCCCTG GCCCACCCTC GTGACCACCT TCGGCTACGG CGTGGCCTGC TTCAGCCGCT
  661 ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC
  721 AGGAGCGCAC CATCTCTTTC AAGGACGACG GTACCTACAA GACCCGCGCC GAGGTGAAGT
  781 TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG
  841 GCAACATCCT GGGGGCACAA GCTGGAGTAC AACTTCAACA GCCACAACGT CTATATCACG
  901 GCCGACAAGC AGAAGGAACG GCATCAAAGG CTAACTTCAA GATCCGCCAC AACGTTGAGG
  961 ACGGCAGCGT GCAGCTTCGC CCGACCACTA CCCAGCAGAA CACCCCCATC GGCCGACCGA
 1021 CCCCGTGCTG CTGGCCCGGA CCACAACTAC TGAGTCATCA GTTCCAGCTG AAGTAAAGAC
1081 CCCCAACCGA GAAAGTCGCG GATTCACT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

2019.07.12

GNP_filter3_RDBDEP_html_190431.pl