Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK376 (Hygro-P2A-mClover)

A plasmid for construction of N-terminal Hygro-P2A-mClover tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16814_A8Lpp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 Region: T7 pro,insert 5'(Hyg R 5') Sequence file: RDB16814_A8Lpd.seq
>D06157B1_A8LP_2_pUC-M13_-100_C04_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNT GGGNANGCNN GGGNTTTCCC NGTCNCGACN TTGTAAAACG    61 ACGGCCAGTG NGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGGTAC CGGGCCCCCC   121 CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGCCACCA TGAAAAAGCC TGAACTCACC   181 GCGACGTCTG TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTCTCCGA CCTGATGCAG   241 CTCTCGGAGG GCGAAGAATC TCGTGCTTTC AGCTTCGATG TAGGAGGGCG TGGATATGTC   301 CTGCGGGTAA ATAGCTGCGC CGATGGTTTC TACAAAGATC GTTATGTTTA TCGGCACTTT   361 GCATCGGCCG CGCTCCCGAT TCCGGAAGTG CTTGACATTG GGGAATTCAG CGAGAGCCTG   421 ACCTATTGCA TCTCCCGCCG TGCACAGGGT GTCACGTTGC AAGACCTGCC TGAAACCGAA   481 CTGCCCGCTG TTCTGCAGCC GGTCGCGGAG GCCATGGATG CGATCGCTGC GGCCGATCTT   541 AGCCAGACGA GCGGGTTCGG CCCATTCGGA CCGCAAGGAA TCGGTCAATA CACTACATGG   601 CGTGATTTCA TATGCGCGAT TGCTGATCCC CATGTGTATC ACTGGCAAAC TGTGATGGAC   661 GACACCGTCA GTGCGTCCGT CGCGCANGCT CTCGATGANC TGATGCTTTG GGCCGAGGAC   721 TGCCCCGAAG TCCGGCACCT CGTGCACGCG GATTTCGGCT CCAACAATGT CCTGACGGNC   781 AATGGCCGCA TAACAGCGGT CATTGACTGG ANCGAGGCGA TGTTCGGGGN TTCCCAATAC   841 GANGTCGCNN ACNTCTTCTT CTGGANGNNG NGGTTGGCTN GTATNGANCA NCANNNGCGC   901 TACTTCNANN NNNNNNNTNN NNNNCTTNNN NNNNCNNNNN NGNNNNNGGN NNANNNNNNN 961 NNN //
Primer: reverse Region: Linker,insert 3'(mClover 3'(A206K)) Sequence file: RDB16814_A8Lpe.seq
>D06157B1_A8LP_2_reverse_D04_10_ABI24.ab1     1 NNNNNNNNNN NNNNNCNNNA ANGGGNNNNN NNGCTGGAGC TCCNCCGCGG TGGCGGCCGC    61 TCTAGAACTA GTGGATCCGG CGCCTGCACC AGCGCCCTTG TACAGCTCGT CCATGCCATG   121 TGTAATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG   181 GTCTTTGCTC AGCTTGGACT GATGGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC   241 GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCTCAAC   301 GTTGTGGCGG ATCTTGAAGT TAGCCTTGAT GCCGTTCTTC TGCTTGTCGG CCGTGATATA   361 GACGTTGTGG CTGTTGAAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT   421 GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC   481 GGCGCGGGTC TTGTAGGTAC CGTCGTCCTT GAAAGAGATG GTGCGCTCCT GGACGTAGCC   541 TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGCTGAA   601 GCAGGCCACG CCGTANCCGA AGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC   661 GGTGGTGCAG ATGAACTTCA GGGTCAGCTT GCCGTTGGTG GCATCGCCCT CGCCCTCGCC   721 GCGGANGCTG AACTTGTGGC CGTTTACGTC GCCGTCCAGC TCGACCAGGA TGGGCACCAC   781 CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC CATAGATCTA GGNCCAGGGT TCTCCTCCAC   841 NNCTNNNGCC TGCTTCAGCA NGCTGAAGTT AGTAGCTCCG CTNNCNGATT CCTTNGNCNN   901 CNGANNANNG CTGGGGGCNN CNGNNTTNCN CTATCNNNNA NNANTTCTAC NNANNNNTCG 961 NNNCNNANNN NNNNNNN //
Primer: HygR-C_F Region: insert mid(Hyg R 3',P2A,mClover 5') Sequence file: RDB16814_A8Lpf.seq
>D06157B1_A8LP_2_HygR-C_F_E04_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GNNGTCGCCN ACNTCTTCTT CTGGANGCCG TGGTTGGCTT    61 GTATGGAGCA GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCGC   121 GGCTCCGGGC GTATATGCTC CGCATTGGTC TTGACCAACT CTATCAGAGC TTGGTTGACG   181 GCAATTTCGA TGATGCAGCT TGGGCGCAGG GTCGATGCGA CGCAATCGTC CGATCCGGAG   241 CCGGGACTGT CGGGCGTACA CAAATCGCCC GCAGAAGCGC GGCCGTCTGG ACCGATGGCT   301 GTGTAGAAGT ACTCGCCGAT AGTGGAAACC GACGCCCCAG CACTCGTCCG AGGGCAAAGG   361 AATCAGGAAG CGGAGCTACT AACTTCAGCC TGCTGAAGCA GGCTGGAGAC GTGGAGGAGA   421 ACCCTGGACC TAGATCTATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA   481 TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTCCGC GGCGAGGGCG   541 AGGGCGATGC CACCAACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC   601 CCGTGCCCTG GCCCACCCTC GTGACCACCT TCGGCTACGG CGTGGCCTGC TTCAGCCGCT   661 ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA NGCTACGTCC   721 AGGAGCGCAC CATCTCTTTC AAGGACGACN GTACCTACAA GACCCGCGCC GANGTGAAGT   781 TCGAGGGCGA NACCCTGGTG AACCGCATCG AGCTGAANGG CATCGACTTC AANGAGGANG   841 GCAACATCCN GGGGGCACAN NCTGGANTAC NACNTNNACA GCCNCAACGN CTATATCANN 901 GNCGANNANN AGAANNANCG GNNTCNANNN NTNNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.02.04

GNP_filter3_RDBDEP_html_220112.pl