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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK322

Donor plasmid to generate TOP2A-mAID degron cell lines with mClover as a marker, hygromycin resistance.

Catalog number RDB18361
Resource name pMK322
Alternative name TOP2A-mAC(Hygro)
Clone info. Donor plasmid to generate TOP2A-mAID degron cell lines with mClover as a marker, hygromycin resistance, see Fig. 5 and SFig. 8 of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone pBluescriptII KS- (plasmid)
Selectable markers Ampicillin (E. coli), Hygromycin (mammalian cell).
Gene/insert name human TOP2A genomic Arabidopsis thaliana IAA17 cDNA Aequorea victoria GFP (mClover) cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank

External Database
human TOP2A Arabidopsis thaliana IAA17 Aequorea victoria GFP (mClover)

        Reference sequence
          
         
        Sequence (full) RDB18361zzk01.seq provided by the depositor

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
        Ordering Please visit Information of Request for Distribution.[link] 
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        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
        提供案内 (日本国内) [open/close]

        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB18361 pMK322 DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Original reference

        original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Auxin Inducible Degron (AID) System (English text)
        Featured content Auxin Inducible Degron (AID) System (Japanese text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB18361_B1K1p1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: pUC-CAPbs_up_F2
        Region: hTOP2A_1, linker, mAID 5'
        Sequence file: RDB18361_B1K1a.seq check
        >18361_18361_B1K1_3_pUC-CAPbs_up_F2_A01_01_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNTNNNNNNN TTATGCTTCC GGCTCGTATG TTGTGTGGAA
           61 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTCG
          121 GCGCGCCATT GGGATGGAAC GAAAACTCCT TGAATATAGT GTCTTGATAC ATTTTTTTAC
          181 ATTACAATTA TGTTGTCAGA TTTACAATGT GCAAGTTACC TGGGCTTTTC TCTTTTAGAA
          241 ATCCAAGGGG GAGAGTGATG ACTTCCATAT GGACTTTGAC TCAGCTGTGG CTCCTCGGGC
          301 AAAATCTGTA CGGGCAAAGA AACCCATTAA ATACCTGGAA GAGTCAGATG AAGATGATCT
          361 GTTTGGATCC GGTGCAGGCG CCAAGGAGAA GAGTGCTTGT CCTAAAGATC CAGCCAAACC
          421 TCCGGCCAAG GCACAAGTTG TGGGATGGCC ACCGGTGAGA TCATACCGGA AGAACGTGAT
          481 GGTTTCCTGC CAAAAATCAA GCGGTGGCCC GGAGGCGGCG GCGTTCGTGA AGGTATCAAT
          541 GGACGGAGCA CCGTACTTGA NGAAAATCGA TTTGAGGATG TATAAAGCTA GCATGGTGAG
          601 CAAGGGCGAN GANCTGTTCN CCGGGGTGGT GNCCATCCNG NNCGAGCTGG ANGGCGACNT
          661 AAACNGNCNC NAGTTCNNCG TNCGCNGCNA GGGNNANGGC NANGCCNCNA NNNNNNNGCT
        721 NANCCTGAAN TTNNNCNNNN NNNNNN
        //
        Primer: pUC-M13_-100
        Region: hTOP2A_2, loxP-2, mPGK ter
        Sequence file: RDB18361_B1K1b.seq check
        >18361_18361_B1K1_3_pUC-M13_-100_B01_04_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNTTTCCNN GTCNCGACGT TGTAAAACGA CGGCCAGTGA
           61 ATTGACGCGT ATTGGGATGG AACGCCCATG AGATGGTCAC TATTTAGACA GTATTATAAA
          121 AAGCTAAAGA ACACTTGGGC TTTACTTCAC TTTGATGTCT TGTACTAAAA ACACCTTCCC
          181 CAAACTAAAT TCAGAGGGGA GGAAGTTAAG AGCTTCAGGT AACTTTAAAA CCAGTCTTGG
          241 GCTTGGTAAG ATAATTACTT AAAATAATCG CCTCACATTT TAAAACAGAT CATCTTCATC
          301 TGACTCTGGA TCCTAGTGAA CCTCTTCGAG GGACCTAATA ACTTCGTATA GCATACATTA
          361 TACGAAGTTA TATTAAGGGT TCCGGATCTC GACCAGCTTC TGATGGAATT AGAACTTGGC
          421 AAAACAATAC TGAGAATGAA GTGTATGTGG AACAGAGGCT GCTGATCTCG TTCTTCAGGC
          481 TATGAAACTG ACACATTTGG AAACCNNNNN NCTTAGAACC ACAAAGTGGG AATCAAGAGA
          541 AAAACAATGA TCCCACGAGA GATCTATAGA TCTATAGATC ATGAGTGGGA GGAANGAGCT
          601 GGNCCTTAAT TNGGNTTNGN NTGNTTAANT NANNATATCN NANTATGAAA NNNTATCNTA
          661 AAGNANNNNN NNAAGAGCCT TCNNTAAAGA NNNNNNNTTN NTCTNATCCN NNCCCNNNCC
        721 CCNNNCCCNN NNNNNNNCNN NNNNCTTNNN NNNNNNN
        //
        Primer: PGKpro_R
        Region: PGK pro, loxP-1. SV40 pA, mClover(A206K) 3'
        Sequence file: RDB18361_B1K1c.seq check
        >18361_18361_B1K1_3_PGKpro_R_C11_08_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNG NNGNGTGCGA NGCCNGANGC CNNTTGTGTA GCGCCNAGTG
           61 CCCAGCGGGG CTGCTAAAGC GCATGCTCCA GACTGCCTTG GGAAAAGCGC CTCCCCTACC
          121 CGGTAGAATT TCGAGGTCGA GATCCTAAGC TTGGCTGGAC GTAAACTCCT CTTCAGACCT
          181 AATAACTTCG TATAGCATAC ATTATACGAA GTTATATTAA GGGTTATTGA ATATGATCGA
          241 CGCGTTAAGA TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG
          301 CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA
          361 ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA
          421 GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG ATCAGTTATC
          481 TAGATTACTT GTACAGCTCG TCCATGCCAT GTGTAATCCC GGCGGCGGTC ACGAACTCCA
          541 GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT CAGCTTGGAC TGATGGCTCA
          601 GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT GGGGGTGTTC TGCTGGTAGT
          661 GGTCGGCGAG CTGCACGCTG CCGTCCTCAA CGTTGTGGCG GATCTTGAAG TTAGCCTTGA
          721 TGCCGTTCTT CTGCTTGTCG GCCGTGATAT AGACGTTGTG GCTGTTGAAG TTGTACTCCA
          781 GCTTGTGCCN CANGATGTTG CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC TCGATGCGGT
          841 TCACCNNGGT GTCGCNCTCN AACTTCANCT CNGCGCGGGT CTTGNNNNNA CCNNCGNNNN
          901 NGAAAGAGAN GGTGCGNTNN TGGACGTANC CNTCNGGCNT GGNNGANTTN NNNNNGNNGN
        961 GCTNCNNCAT GNGNNNNGGG NANCNGNNTG NNNNNNNNNN
        //
        Primer: PGKpro_F
        Region: HygR
        Sequence file: RDB18361_B1K1d.seq check
        >18361_18361_B1K1_3_PGKpro_F_E01_13_ABI24.ab1
            1 NNNNNNNNNN NNNNNNTCTC CNGGCCTTTC GACCTGCATC CNTCTAGATC TCGAGCAGCT
           61 GAAGCTTACC GCTAGCATGG ATAGATCCGG AAAGCCTGAA CTCACCGCGA CGTCTGTCGA
          121 GAAGTTTCTG ATCGAAAAGT TCGACAGCGT CTCCGACCTG ATGCAGCTCT CGGAGGGCGA
          181 AGAATCTCGT GCTTTCAGCT TCGATGTAGG AGGGCGTGGA TATGTCCTGC GGGTAAATAG
          241 CTGCGCCGAT GGTTTCTACA AAGATCGTTA TGTTTATCGG CACTTTGCAT CGGCCGCGCT
          301 CCCGATTCCG GAAGTGCTTG ACATTGGGGA ATTCAGCGAG AGCCTGACCT ATTGCATCTC
          361 CCGCCGTGCA CAGGGTGTCA CGTTGCAAGA CCTGCCTGAA ACCGAACTGC CCGCTGTTCT
          421 GCAGCCGGTC GCGGAGGCCA TGGATGCGAT CGCTGCGGCC GATCTTAGCC AGACGAGCGG
          481 GTTCGGCCCA TTCGGACCGC AAGGAATCGG TCAATACACT ACATGGCGTG ATTTCATATG
          541 CGCGATTGCT GATCCCCATG TGTATCACTG GCAAACTGTG NTGGACGACA CCGTCAGTGC
          601 GTCCGTCGCG CANGCTCTCG ATGANCTGAN GCTTTGGGCC GANGANTGNC CCNAAGTCCG
          661 GCACCTCNNG CACNCNGNNT TCNGCTNCNN CNNTGNCCNG ANNNNNNNNN GNNNGCNTNN
        721 NNNNNGTCNN NNNNNNNN
        //
        Primer: EGFP-C-rev
        Region: mClover(A206K) 5', mAID 3'
        Sequence file: RDB18361_B1K1e.seq check
        >18361_18361_B1K1_3_EGFP-C-rev_F01_06_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNTTGGNN TGNNGGCTCN NNGNNNNGGT
           61 TGTCGGGCAG CANCNCGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA
          121 GCTGCACGCT GCCGTCCTCA ACGTTGTGGC GGATCTTGAA GTTAGCCTTG ATGCCGTTCT
          181 TCTGCTTGTC GGCCGTGATA TAGACGTTGT GGCTGTTGAA GTTGTACTCC AGCTTGTGCC
          241 CCAGGATGTT GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG
          301 TGTCGCCCTC GAACTTCACC TCGGCGCGGG TCTTGTAGGT ACCGTCGTCC TTGAAAGAGA
          361 TGGTGCGCTC CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA
          421 TGTGGTCGGG GTAGCGGCTG AAGCAGGCCA CGCCGTAGCC GAAGGTGGTC ACGAGGGTGG
          481 GCCAGGGCAC GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTTGG
          541 TGGCATCGCC CTCGCCCTCG CCGCGGACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA
          601 GCTCGACCAG GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGCTAG
          661 CTTTATACAT CCTCAAATCG ATTTTCCTCN AGTACGGTGC TCCGTNNNNN GATACCTTCA
          721 CGAACGCCGC CGCCTCCGGG CCACCGCTTG ATTTTTGGCA NGAAANCATC ACGTTCTTCC
          781 GGTATGATCT CNNCGGNGGC CATCNCNCNN CTTGTGCCTT GGCCGGANGT TTGGCTGGAT
          841 CTTTANGANA AGCACTCNTT CTCCTNNGCG NCNGCACCGG NNCCAAACAG NATCATCTTC
          901 ATCTGACTNT NNNANGTATT TNANNGGGGN TTCTTTNNCC CGNNNNNGAT TTTNNNNNNN
          961 AGGANNNNNC NNGNNGNANN NNNNANNNCN ANATNGNAAA NNCNNTNNNC NNNNNNNNNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522.

        2022.08.12

        GNP_filter3_RDBDEP_html_220812.pl