A plasmid for construction of C-terminal mAID-mClover tagging donors.
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External Database Arabidopsis thaliana AtIAA17 Aequorea victoria GFP Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
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Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ![]() |
提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。 |
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提供依頼 | 手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word ![]() 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word ![]() |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB13933 | pMK290 (mAID-mClover-Hygro) | DNA solution |
Materials & Methods section:
The pMK290 (mAID-mClover-Hygro) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13933). |
Reference section:
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Auxin Inducible Degron (AID) System (English text) |
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Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13933_A6B8p1-3.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: M13_-40 (Pr0410) Region: insert mid(Linker,mAID,mClover(A206K) 5') Sequence file: RDB13933_A6B8i.seq ![]() |
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>D03070A17_A6B8_1_M13(-40)_B05_05.ab1 1 NNNNNNNNNN NNNNCNNANA NNNNTNNNNA TAGGGCGAAN TGGAGCTCCC CGGGNTCCGG 61 TGCAGGCGCC AAGGAGAAGA GTGCTTGTCC TAAAGATCCA GCCAAACCTC CGGCCAAGGC 121 ACAAGTTGTG GGATGGCCAC CGGTGAGATC ATACCGGAAG AACGTGATGG TTTCCTGCCA 181 AAAATCAAGC GGTGGCCCGG AGGCGGCGGC GTTCGTGAAG GTATCAATGG ACGGAGCACC 241 GTACTTGAGG AAAATCGATT TGAGGATGTA TAAAGCTAGC ATGGTGAGCA AGGGCGAGGA 301 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA 361 GTTCAGCGTC CGCGGCGAGG GCGAGGGCGA TGCCACCAAC GGCAAGCTGA CCCTGAAGTT 421 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCTTCGGCTA 481 CGGCGTGGCC TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC 541 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTCT TTCAAGGACG ACGGTACCTA 601 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA 661 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTTCAA 721 CAGCCACAAC GTCTATATCA CGGCCGACAA GCAGANNAAC GGCATCNAGG CTAACTTCAA 781 GATCCGNCAC NACGTTGAGG ACGGCAGCNN GCANCTNNGC CGACCACNAC NAGCANANNA 841 CCCCCATCGG CGACGGNCCC GNGCTGCTGC NNNACNACNN NTACCTNANC CNTCAGTNNN 901 AGCTGAGCAA ANACCCCANN NANNANCGCN NNTNANNNNG NNNCNTGCNN GNNNTTCGTG 961 ACNNNCNNNC NGGNNNNNNN NNN // |
Primer: M13R (Pr0160) Region: insert 3'(loxP,mPGK ter,HygR 3') Sequence file: RDB13933_A6B8j.seq ![]() |
>D03070A17_A6B8_1_M13R_B04_04.ab1 1 NNNNNNNNNN NNNNNNNNNN NNAAGGGNNN AAAAGCTGGG TACCGGATCC TAGTGAACCT 61 CTTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC GAAGTTATAT TAAGGGTTCC 121 GGATCTCGAC CAGCTTCTGA TGGAATTAGA ACTTGGCAAA ACAATACTGA GAATGAAGTG 181 TATGTGGAAC AGAGGCTGCT GATCTCGTTC TTCAGGCTAT GAAACTGACA CATTTGGAAA 241 CCACAGTACT TAGAACCACA AAGTGGGAAT CAAGAGAAAA ACAATGATCC CACGAGAGAT 301 CTATAGATCT ATAGATCATG AGTGGGAGGA ATGAGCTGGC CCTTAATTTG GTTTTGCTTG 361 TTTAAATTAT GATATCCAAC TATGAAACAT TATCATAAAG CAATAGTAAA GAGCCTTCAG 421 TAAAGAGCAG GCATTTATCT AATCCCACCC CACCCCCACC CCCGTAGCTC CAATCCTTCC 481 ATTCAAAATG TAGGTACTCT GTTCTCACCC TTCTTAACAA AGTATGACAG GAAAAACTTC 541 CATTTTAGTG GACATCTTTA TTGTTTAATA GATCATCAAT TTCTGCATCC CGGGGATCTG 601 ATATCATCGA TGCATGGGGT CGTGCGCTCC TTTCGGTCGG GCGCTGCGGG TCGTGGGGCG 661 GGCGGCTAGC CTATTCCTTT GCCCTCGGAC GAGTGCTGGG GCGTCGGTTT CCACTATCGG 721 CGAGTACTTC TACACAGCCA TCGGTCCAGA CNGCCGCGCT TCTGCGGGCG ANTTGTGTAC 781 GCCCGACAGT CCCGGCTCCG GATCGGANGA TTGCGTCGCA TCGACCCTGC GCCCNAGCTG 841 CATCATCNAA ATTGCCGTCN ACCNAGCTCT GANAGANTTN NTCAAGANCA ATGCGGANCA 901 TATACNCCNG GANCCNCGGC NNTCCTGNNA GCNNNNGANG CCNNCGCTCN NAGTNNNNNG 961 NNTGNNGCNN NNNANNNGCC NANCNNNGGN CNNNNNNNNN N // |
Primer: PGK_N_rev (Pr0243) Region: insert mid(loxP,SV40 pA,mClover(A206K) 3') Sequence file: RDB13933_A6B8k.seq ![]() |
>D03070A17_A6B8_1_PGK-N-rev_E06_15.ab1 1 NNNNNNNNNN NNNNNCNNNN NNNNANNNNT TCNAGGTCGA GATCCTAAGC TTGGCTGGAC 61 GTAAACTCCT CTTCAGACCT AATAACTTCG TATAGCATAC ATTATACGAA GTTATATTAA 121 GGGTTATTGA ATATGATCGA CGCGTTAAGA TACATTGATG AGTTTGGACA AACCACAACT 181 AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA 241 ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG 301 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG 361 GCTGATTATG ATCAGTTATC TAGATTACTT GTACAGCTCG TCCATGCCAT GTGTAATCCC 421 GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT 481 CANCTTGGAC TGATGGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT 541 GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCNN NNNNCCTCAA CGTTGTGGCG 601 GATCTTGAAG TTANNNNNNN NNN // |
Primer: PGKpro_F (Pr0249) Region: HygR 5' Sequence file: RDB13933_A6B8l.seq ![]() |
>D03070A17_A6B8_1_PGK-proF_H07_22.ab1 1 NNNNNNNNNN NNNNNNNNNN NTCTCCNGGC CTTTCGACCT GCATCCNTCT AGATCTCGAG 61 CAGCTGAAGC TTACCGCTAG CATGGATAGA TCCGGAAAGC CTGAACTCAC CGCGACGTCT 121 GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA GCTCTCGGAG 181 GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT CCTGCGGGTA 241 AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT TGCATCGGCC 301 GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT GACCTATTGC 361 ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA ACTGCCCGCT 421 GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT TAGCCAGACG 481 AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG GCGTGATTTC 541 ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA CGACACCGTC 601 AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA CTGCCCCGAA 661 GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCNANAATG TCCTGACGGA CAATGGCCGC 721 ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG ATTCCCAATA CGAGGTCGCC 781 AACATCTTCT TCTGGANGCC GTGGTTGGCT TGTATGGAGC AGCANACGCG CTACTTCGNG 841 CGGANGCNTC CGGAGCTNGC NNNTCGCCGC NGCTCCNNNC GTATATGCTC CGCNTTNNNC 901 NTGANCAACT CTATCANAGC TNGNNTNGAN NNCNNTTTNN ATGANGCANC TNNNGNGCNN 961 NNTCGANGNN ACGCNNNCNN NCCNNNNCNG ANNCNNGNNN NGNNNNGGNG NNNNNNANNN 1021 NNNNNNN // |
Primer: RV5F (pGL3_RV5F) (Pr0227) Region: insert mid(mClover(A206K) mid,mAID 3') Sequence file: RDB13933_A6B8m.seq ![]() |
>13933_13933_B2JhF_RV5F_C02_03_ABI08.ab1 1 NNNNNNNNNN NNNNNGNNNN NGGNTGNNTN NGNNNNGTTA TCTAGATTAC TTGTACAGCT 61 CGTCCATGCC ATGTGTAATC CCGGCGGCGG TCACGAACTC CAGCAGGACC ATGTGATCGC 121 GCTTCTCGTT GGGGTCTTTG CTCAGCTTGG ACTGATGGCT CAGGTAGTGG TTGTCGGGCA 181 GCAGCACGGG GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC 241 TGCCGTCCTC AACGTTGTGG CGGATCTTGA AGTTAGCCTT GATGCCGTTC TTCTGCTTGT 301 CGGCCGTGAT ATAGACGTTG TGGCTGTTGA AGTTGTACTC CAGCTTGTGC CCCAGGATGT 361 TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCCT 421 CGAACTTCAC CTCGGCGCGG GTCTTGTAGG TACCGTCGTC CTTGAAAGAG ATGGTGCGCT 481 CCTGGACGTA GCCTTCGGGC ATGGCGGACT TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG 541 GGTAGCGGCT GAAGCAGGCC ACGCCGTAGC CGAAGGTGGT CACGAGGGTG GGCCAGGGCA 601 CGGGCAGCTT GCCGGTGGTG CAGATGAACT TCAGGGTCAG CTTGCCGTTG GTGGCATCGC 661 CCTCGCCCTC GCCGCGGACG CTGAACTTGT GGCCGTTTAC GTCGCCGTCC AGCTCGACCA 721 GGATGGGCAC CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACCATGCTA GCTTTATACA 781 TCCTCAAATC GATTTTCCTC AAGTACGGTG CTCCGTCCAT TGATACCTTC ACGAACGCCG 841 CCGCCTCCNG NNCANCGCTT GANTTTTGGC NNNAACCATC ACGTTCNTNC CNGTATGATC 901 TCACNNGNGG NCATCCCACA NCTTGTGCCT TGGCCNGNNG NTTTGGCNNG NATCTTTNNN 961 NNAGCACTCT TNNCCTNNGN CNNNNNNNNN CNGNNTNCNN GNNNNNNNNN N // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. |
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reference | Goto, H., Chk1-mediated Cdc25A degradation as a critical mechanism for normal cell cycle progression. J Cell Sci. 2019 Jan 25;132(2). pii: jcs223123 (2019). PMID 30635443. |
reference | Devrekanli, A., Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes. Methods in Molecular Biology 1369: 257-278 (2016). PMID 26519318. |
reference | Takagi, M., Perichromosomal protein Ki67 supports mitotic chromosome architecture. Genes Cells 21 (10): 1113-1124 (2016). PMID 27610954. |
reference | Nishimura, K., Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Curr. Protoc. Cell Biol. \64: 20.9.1-16 (2014). PMID 25181302. |
2023.05.01
GNP_filter3_RDBDEP_html_230501.pl