RDB13933 - pMK290 (mAID-mClover-Hygro)

Resource data sheet
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pMK290 (mAID-mClover-Hygro)

A plasmid for construction of C-terminal mAID-mClover tagging donors.


Catalog number	RDB13933
Resource name	pMK290 (mAID-mClover-Hygro)
Alternative name	mAID-mClover-Hygro, 1025
Clone info.	A plasmid for construction of C-terminal mAID-mClover tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone. To make 0.5 M stock solution, dissolve indole-3-acetic acid (IAA) (Sigma-Aldrich, 45533) or 1-naphthaleneacetic acid (NAA) (Sigma-Aldrich, 35745) in 100 % DMSO. IAA solution can be made in 100 % ethanol. Aliquots can be kept for 6 months in the dark at -20oC (Devrekanli, A., Kanemaki, M.T. Methods in Molecular Biology, 1369, 257-278, 2016).
Vector backbone	pBluescriptII KS- (Plasmid)
Size of vector backbone	3.0 kb
Selectable markers	Am^r
Gene/insert name	Arabidopsis thaliana AtIAA17 cDNA Aequorea victoria GFP cDNA
Depositor	Kanemaki, Masato \|
Other clones in our bank	Arabidopsis thaliana AtIAA17 (KEGG orthology K14484) \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

Ordering Please visit Information of Request for Distribution.
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]

Remarks
Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13933	pMK290 (mAID-mClover-Hygro)	DNA solution

cDNA Request [in Japanese] [in English]

References and tips

Electronic file

Featured content	Auxin Inducible Degron (AID) System (English text)
Featured content	Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB13933_A6B8p1_v2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40	RDB13933_A6B8a_2.seq
>D03070A17_A6B8_1_M13(-40)_B05_05.ab1 1 CCGCCGCTAA CGCGCGTATA CGACTCACTA TAGGGCGAAT TGGAGCTCCC CGGGATCCGG 61 TGCAGGCGCC AAGGAGAAGA GTGCTTGTCC TAAAGATCCA GCCAAACCTC CGGCCAAGGC 121 ACAAGTTGTG GGATGGCCAC CGGTGAGATC ATACCGGAAG AACGTGATGG TTTCCTGCCA 181 AAAATCAAGC GGTGGCCCGG AGGCGGCGGC GTTCGTGAAG GTATCAATGG ACGGAGCACC 241 GTACTTGAGG AAAATCGATT TGAGGATGTA TAAAGCTAGC ATGGTGAGCA AGGGCGAGGA 301 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA 361 GTTCAGCGTC CGCGGCGAGG GCGAGGGCGA TGCCACCAAC GGCAAGCTGA CCCTGAAGTT 421 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCTTCGGCTA 481 CGGCGTGGCC TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC 541 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTCT TTCAAGGACG ACGGTACCTA 601 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA 661 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTTCAA 721 CAGCCACAAC GTCTATATCA CGGCCGACAA GCAGAAGAAC GGCATCAAGG CTAACTTCAA 781 GATCCGCCAC AACGTTGAGG ACGGCAGCGT GCAGCTCGGC CGACCACTAC CAGCAGAACA 841 CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC CATCAGTCCA 901 AGCTGAGCAA AGACCCCAAC GAGAAGCGCG AATCACATGG GTCCCTGCTG GGAGTTCGTG 961 ACCGCCCGCC CGGGATTACC ACTTGGGCAA TGGGAACGAG CTTGTACCAG GTTAATCCTA 1021 GATAAACTGA TCATAAATCC AGCGCATAAC CAACAATTTG GTAGAAGGTT TTACTTGGCT 1081 TAAAAAAACC CTCCCACAAC CTTCCACCCT GAACCTGAAA CACATAAAAA TGAATTGCCA 1141 ACTGTGGTTG TTAAACCTTG GATTATATTG GCCAGCCTTT AATATATGCG AGTA //
Primer: PGK_N_rev	RDB13933_A6B8b_2.seq
>D03070A17_A6B8_1_PGK-N-rev_E06_15.ab1 1 CATGGGGAAC GCCTCCCTAC CGGTAGAATT TCGAGGTCGA GATCCTAAGC TTGGCTGGAC 61 GTAAACTCCT CTTCAGACCT AATAACTTCG TATAGCATAC ATTATACGAA GTTATATTAA 121 GGGTTATTGA ATATGATCGA CGCGTTAAGA TACATTGATG AGTTTGGACA AACCACAACT 181 AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA 241 ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG 301 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG 361 GCTGATTATG ATCAGTTATC TAGATTACTT GTACAGCTCG TCCATGCCAT GTGTAATCCC 421 GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT 481 CAGCTTGGAC TGATGGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT 541 GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCAA CGTTGTGGCG 601 GATCTTGAAG TTAGCCTTGA TGCCGTTCTT CTGCTTGTCG GCCGTGATAT AGACGTTGTG 661 GCTGTTGAAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAAGTCGA 721 TGCCCTTCAG CTCGATGCGG TTCACCAGGG GTGTCGCCCT //
Primer: PGKpro_F	RDB13933_A6B8c_2.seq
>D03070A17_A6B8_1_PGK-proF_H07_22.ab1 1 ATCGGCGTAT CTCTCTTCTC ATCTCCGGGC CTTTCGACCT GCATCCATCT AGATCTCGAG 61 CAGCTGAAGC TTACCGCTAG CATGGATAGA TCCGGAAAGC CTGAACTCAC CGCGACGTCT 121 GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA GCTCTCGGAG 181 GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT CCTGCGGGTA 241 AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT TGCATCGGCC 301 GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT GACCTATTGC 361 ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA ACTGCCCGCT 421 GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT TAGCCAGACG 481 AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG GCGTGATTTC 541 ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA CGACACCGTC 601 AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA CTGCCCCGAA 661 GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG TCCTGACGGA CAATGGCCGC 721 ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG ATTCCCAATA CGAGGTCGCC 781 AACATCTTCT TCTGGAGGCC GTGGTTGGCT TGTATGGAGC AGCAGACGCG CTACTTCGAG 841 CGGAGGCATC CGGAGCTTGC AGATCGCCGC GGCTCCGGGC GTATATGCTC CGCATTGGTC 901 TTGACCAACT CTATCAGAGC TTGGTTTGAC GGCAATTTCG ATGATGCAGC TTGGGCGCAG 961 GGTCGATGCG ACGCAATCGT TCCGATCCGG AGCCCGGGAC TGTCCGGGCG TACACAAATT 1021 CGCCGCCAGA AGCGCGGCCC GTCTGGACCG ATGCTGGTGT AGAAGTTACT CCGCCGGAAT 1081 AGTGGAATCG GACGTCCCCA GCCACTCGTT CGAAGGTCTA GATAGCCTAG CCGCCCGCTC 1141 CACGAACCCG CTAGCGCGTC //
Primer: M13R	RDB13933_A6B8d_2.seq
>D03070A17_A6B8_1_M13R_B04_04.ab1 1 CAGGCCGGCA TTACCCTCAC TAAAGGGAAC AAAAGCTGGG TACCGGATCC TAGTGAACCT 61 CTTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC GAAGTTATAT TAAGGGTTCC 121 GGATCTCGAC CAGCTTCTGA TGGAATTAGA ACTTGGCAAA ACAATACTGA GAATGAAGTG 181 TATGTGGAAC AGAGGCTGCT GATCTCGTTC TTCAGGCTAT GAAACTGACA CATTTGGAAA 241 CCACAGTACT TAGAACCACA AAGTGGGAAT CAAGAGAAAA ACAATGATCC CACGAGAGAT 301 CTATAGATCT ATAGATCATG AGTGGGAGGA ATGAGCTGGC CCTTAATTTG GTTTTGCTTG 361 TTTAAATTAT GATATCCAAC TATGAAACAT TATCATAAAG CAATAGTAAA GAGCCTTCAG 421 TAAAGAGCAG GCATTTATCT AATCCCACCC CACCCCCACC CCCGTAGCTC CAATCCTTCC 481 ATTCAAAATG TAGGTACTCT GTTCTCACCC TTCTTAACAA AGTATGACAG GAAAAACTTC 541 CATTTTAGTG GACATCTTTA TTGTTTAATA GATCATCAAT TTCTGCATCC CGGGGATCTG 601 ATATCATCGA TGCATGGGGT CGTGCGCTCC TTTCGGTCGG GCGCTGCGGG TCGTGGGGCG 661 GGCGGCTAGC CTATTCCTTT GCCCTCGGAC GAGTGCTGGG GCGTCGGTTT CCACTATCGG 721 CGAGTACTTC TACACAGCCA TCGGTCCAGA CGGCCGCGCT TCTGCGGGCG ATTTGTGTAC 781 GCCCGACAGT CCCGGCTCCG GATCGGACGA TTGCGTCGCA TCGACCCTGC GCCCAAGCTG 841 CATCATCGAA ATTGCCGTCA ACCAAGCTCT GATAGAGTTG GTCAAGACCA ATGCGGAGCA 901 TATACGCCCG GAGCCGCGGC GATCCTGCAA GCTCCGGATG CCTCCGCTCG AAGTAGCGCG 961 TCTGCTGCTC CATACAAGCC AACCCACGGC CTCCAGAAGA AGATGTTGGG CGGACCTCGT 1021 ATTGGGGAAA TCCCCCGAAC ATCGCCTCGC CTCCAGTCAA ATGACCCGCT GGTATGGCCG 1081 GCCATGGTCG TCAGGGACAT TGGTGGAGTG AATCCGCGGT GCACGAAGGG TGGCCGGAAC 1141 CTTCCGGGGG GC //

Please visit Sequencing and PCR primers for primer information.

References

original	Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.
reference	Goto, H., Chk1-mediated Cdc25A degradation as a critical mechanism for normal cell cycle progression. J Cell Sci. 2019 Jan 25;132(2). pii: jcs223123 (2019). PMID 30635443.
reference	Devrekanli, A., Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes. Methods in Molecular Biology 1369: 257-278 (2016). PMID 26519318.
reference	Takagi, M., Perichromosomal protein Ki67 supports mitotic chromosome architecture. Genes Cells 21 (10): 1113-1124 (2016). PMID 27610954.
reference	Nishimura, K., Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Curr. Protoc. Cell Biol. \64: 20.9.1-16 (2014). PMID 25181302 .

2019.03.08

GNP_filter3_RDBDEP_html_190307.pl