Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK290 (mAID-mClover-Hygro)

A plasmid for construction of C-terminal mAID-mClover tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

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Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13933_A6B8p1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410) Region: insert mid(Linker,mAID,mClover(A206K) 5') Sequence file: RDB13933_A6B8i.seq
>D03070A17_A6B8_1_M13(-40)_B05_05.ab1     1 NNNNNNNNNN NNNNCNNANA NNNNTNNNNA TAGGGCGAAN TGGAGCTCCC CGGGNTCCGG    61 TGCAGGCGCC AAGGAGAAGA GTGCTTGTCC TAAAGATCCA GCCAAACCTC CGGCCAAGGC   121 ACAAGTTGTG GGATGGCCAC CGGTGAGATC ATACCGGAAG AACGTGATGG TTTCCTGCCA   181 AAAATCAAGC GGTGGCCCGG AGGCGGCGGC GTTCGTGAAG GTATCAATGG ACGGAGCACC   241 GTACTTGAGG AAAATCGATT TGAGGATGTA TAAAGCTAGC ATGGTGAGCA AGGGCGAGGA   301 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA   361 GTTCAGCGTC CGCGGCGAGG GCGAGGGCGA TGCCACCAAC GGCAAGCTGA CCCTGAAGTT   421 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCTTCGGCTA   481 CGGCGTGGCC TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC   541 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTCT TTCAAGGACG ACGGTACCTA   601 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA   661 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTTCAA   721 CAGCCACAAC GTCTATATCA CGGCCGACAA GCAGANNAAC GGCATCNAGG CTAACTTCAA   781 GATCCGNCAC NACGTTGAGG ACGGCAGCNN GCANCTNNGC CGACCACNAC NAGCANANNA   841 CCCCCATCGG CGACGGNCCC GNGCTGCTGC NNNACNACNN NTACCTNANC CNTCAGTNNN   901 AGCTGAGCAA ANACCCCANN NANNANCGCN NNTNANNNNG NNNCNTGCNN GNNNTTCGTG 961 ACNNNCNNNC NGGNNNNNNN NNN //
Primer: M13R (Pr0160) Region: insert 3'(loxP,mPGK ter,HygR 3') Sequence file: RDB13933_A6B8j.seq
>D03070A17_A6B8_1_M13R_B04_04.ab1     1 NNNNNNNNNN NNNNNNNNNN NNAAGGGNNN AAAAGCTGGG TACCGGATCC TAGTGAACCT    61 CTTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC GAAGTTATAT TAAGGGTTCC   121 GGATCTCGAC CAGCTTCTGA TGGAATTAGA ACTTGGCAAA ACAATACTGA GAATGAAGTG   181 TATGTGGAAC AGAGGCTGCT GATCTCGTTC TTCAGGCTAT GAAACTGACA CATTTGGAAA   241 CCACAGTACT TAGAACCACA AAGTGGGAAT CAAGAGAAAA ACAATGATCC CACGAGAGAT   301 CTATAGATCT ATAGATCATG AGTGGGAGGA ATGAGCTGGC CCTTAATTTG GTTTTGCTTG   361 TTTAAATTAT GATATCCAAC TATGAAACAT TATCATAAAG CAATAGTAAA GAGCCTTCAG   421 TAAAGAGCAG GCATTTATCT AATCCCACCC CACCCCCACC CCCGTAGCTC CAATCCTTCC   481 ATTCAAAATG TAGGTACTCT GTTCTCACCC TTCTTAACAA AGTATGACAG GAAAAACTTC   541 CATTTTAGTG GACATCTTTA TTGTTTAATA GATCATCAAT TTCTGCATCC CGGGGATCTG   601 ATATCATCGA TGCATGGGGT CGTGCGCTCC TTTCGGTCGG GCGCTGCGGG TCGTGGGGCG   661 GGCGGCTAGC CTATTCCTTT GCCCTCGGAC GAGTGCTGGG GCGTCGGTTT CCACTATCGG   721 CGAGTACTTC TACACAGCCA TCGGTCCAGA CNGCCGCGCT TCTGCGGGCG ANTTGTGTAC   781 GCCCGACAGT CCCGGCTCCG GATCGGANGA TTGCGTCGCA TCGACCCTGC GCCCNAGCTG   841 CATCATCNAA ATTGCCGTCN ACCNAGCTCT GANAGANTTN NTCAAGANCA ATGCGGANCA   901 TATACNCCNG GANCCNCGGC NNTCCTGNNA GCNNNNGANG CCNNCGCTCN NAGTNNNNNG 961 NNTGNNGCNN NNNANNNGCC NANCNNNGGN CNNNNNNNNN N //
Primer: PGK_N_rev (Pr0243) Region: insert mid(loxP,SV40 pA,mClover(A206K) 3') Sequence file: RDB13933_A6B8k.seq
>D03070A17_A6B8_1_PGK-N-rev_E06_15.ab1     1 NNNNNNNNNN NNNNNCNNNN NNNNANNNNT TCNAGGTCGA GATCCTAAGC TTGGCTGGAC    61 GTAAACTCCT CTTCAGACCT AATAACTTCG TATAGCATAC ATTATACGAA GTTATATTAA   121 GGGTTATTGA ATATGATCGA CGCGTTAAGA TACATTGATG AGTTTGGACA AACCACAACT   181 AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA   241 ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG   301 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG   361 GCTGATTATG ATCAGTTATC TAGATTACTT GTACAGCTCG TCCATGCCAT GTGTAATCCC   421 GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT   481 CANCTTGGAC TGATGGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT   541 GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCNN NNNNCCTCAA CGTTGTGGCG 601 GATCTTGAAG TTANNNNNNN NNN //
Primer: PGKpro_F (Pr0249) Region: HygR 5' Sequence file: RDB13933_A6B8l.seq
>D03070A17_A6B8_1_PGK-proF_H07_22.ab1     1 NNNNNNNNNN NNNNNNNNNN NTCTCCNGGC CTTTCGACCT GCATCCNTCT AGATCTCGAG    61 CAGCTGAAGC TTACCGCTAG CATGGATAGA TCCGGAAAGC CTGAACTCAC CGCGACGTCT   121 GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA GCTCTCGGAG   181 GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT CCTGCGGGTA   241 AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT TGCATCGGCC   301 GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT GACCTATTGC   361 ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA ACTGCCCGCT   421 GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT TAGCCAGACG   481 AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG GCGTGATTTC   541 ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA CGACACCGTC   601 AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA CTGCCCCGAA   661 GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCNANAATG TCCTGACGGA CAATGGCCGC   721 ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG ATTCCCAATA CGAGGTCGCC   781 AACATCTTCT TCTGGANGCC GTGGTTGGCT TGTATGGAGC AGCANACGCG CTACTTCGNG   841 CGGANGCNTC CGGAGCTNGC NNNTCGCCGC NGCTCCNNNC GTATATGCTC CGCNTTNNNC   901 NTGANCAACT CTATCANAGC TNGNNTNGAN NNCNNTTTNN ATGANGCANC TNNNGNGCNN   961 NNTCGANGNN ACGCNNNCNN NCCNNNNCNG ANNCNNGNNN NGNNNNGGNG NNNNNNANNN 1021 NNNNNNN //
Primer: RV5F (pGL3_RV5F) (Pr0227) Region: insert mid(mClover(A206K) mid,mAID 3') Sequence file: RDB13933_A6B8m.seq
>13933_13933_B2JhF_RV5F_C02_03_ABI08.ab1     1 NNNNNNNNNN NNNNNGNNNN NGGNTGNNTN NGNNNNGTTA TCTAGATTAC TTGTACAGCT    61 CGTCCATGCC ATGTGTAATC CCGGCGGCGG TCACGAACTC CAGCAGGACC ATGTGATCGC   121 GCTTCTCGTT GGGGTCTTTG CTCAGCTTGG ACTGATGGCT CAGGTAGTGG TTGTCGGGCA   181 GCAGCACGGG GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC   241 TGCCGTCCTC AACGTTGTGG CGGATCTTGA AGTTAGCCTT GATGCCGTTC TTCTGCTTGT   301 CGGCCGTGAT ATAGACGTTG TGGCTGTTGA AGTTGTACTC CAGCTTGTGC CCCAGGATGT   361 TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCCT   421 CGAACTTCAC CTCGGCGCGG GTCTTGTAGG TACCGTCGTC CTTGAAAGAG ATGGTGCGCT   481 CCTGGACGTA GCCTTCGGGC ATGGCGGACT TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG   541 GGTAGCGGCT GAAGCAGGCC ACGCCGTAGC CGAAGGTGGT CACGAGGGTG GGCCAGGGCA   601 CGGGCAGCTT GCCGGTGGTG CAGATGAACT TCAGGGTCAG CTTGCCGTTG GTGGCATCGC   661 CCTCGCCCTC GCCGCGGACG CTGAACTTGT GGCCGTTTAC GTCGCCGTCC AGCTCGACCA   721 GGATGGGCAC CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACCATGCTA GCTTTATACA   781 TCCTCAAATC GATTTTCCTC AAGTACGGTG CTCCGTCCAT TGATACCTTC ACGAACGCCG   841 CCGCCTCCNG NNCANCGCTT GANTTTTGGC NNNAACCATC ACGTTCNTNC CNGTATGATC   901 TCACNNGNGG NCATCCCACA NCTTGTGCCT TGGCCNGNNG NTTTGGCNNG NATCTTTNNN 961 NNAGCACTCT TNNCCTNNGN CNNNNNNNNN CNGNNTNCNN GNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl