Sequencing and PCR primers for primer information.
A donor vector for introducing Tet-OsTIR1 at the human AAVS1 locus.
| Catalog number | RDB13919 |
|---|---|
| Resource name | pMK243 (TetOne OsTIR1 AAVS1) |
| Alternative name | TetOne OsTIR1 AAVS1 |
| Clone info. | A donor vector for introducing Tet-OsTIR1 at the human AAVS1 locus. Please refer Fig. 3 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone. Oryza sativa OsTIR1 (NP_001052659.1), codon optimized cDNA for the expression in human cells. To make 0.5 M stock solution, dissolve indole-3-acetic acid (IAA) (Sigma-Aldrich, 45533) or 1-naphthaleneacetic acid (NAA) (Sigma-Aldrich, 35745) in 100 % DMSO. IAA solution can be made in 100 % ethanol. Aliquots can be kept for 6 months in the dark at -20oC (Devrekanli, A., Kanemaki, M.T. Methods in Molecular Biology, 1369, 257-278, 2016). |
| Vector backbone | pCR4Blunt-TOPO (Plasmid) |
| Size of vector backbone | 4.0 kb |
| Selectable markers | Am^r (E. coli), Puro^r (mammalian cells) |
| Gene/insert name | human AAVS1 genomic Oryza sativa OsTIR1 cDNA |
| Depositor | Kanemaki, Masato | |
| Other clones in our bank | human AAVS1 (NCBI Gene 17) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Please contact us. We will provide you with appropriate documents. |
| Remarks | Please contact us. We will provide you with appropriate documents. Please contact us. We will provide you with appropriate documents. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB13919 | pMK243 (TetOne OsTIR1 AAVS1) | DNA solution |
Electronic file
Featured content
| Featured content | Auxin Inducible Degron (AID) System (English text) |
|---|---|
| Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
References
| original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. |
|---|
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB13919_A6B8p1_v2.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
| Primer: TetOne Oligo 1 | RDB13919_A6B8a_2.seq |
|---|---|
|
>D03070A03_A6B8_1_Tet One oligo1_E09_15.ab1 1 CTGCAGTGAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT 61 TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA 121 GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA 181 TTATGATCCT CTGGAGATTT ACCCGGGGAG CATGTCAAGG TCAAAATCGT CAAGAGCGTC 241 AGCAGGCAGC ATATCAAGGT CAAAGTCGTC AAGGGCATCG GCTGGGAGCA TGTCTAAGTC 301 AAAATCGTCA AGGGCGTCGG TCGGCCCGCC GCTTTCGCAC TTTAGCTGTT TCTCCAGGCC 361 ACATATGATT AGTTCCAGGC CGAAAAGGAA GGCAGGTTCG GCTCCCTGCC GGTCGAACAG 421 CTCAATTGCT TGTTTCAGAA GTGGGGGCAT AGAATCGGTG GTAGGTGTCT CTCTTTCCTC 481 TTTTGCTACT TGATGCTCCT GTTCCTCCAA TACGCAGCCC AGTGTAAAGT GGCCCACGGC 541 GGACAGAGCG TACAGTGCGT TCTCCAGGGA GAAGCCTTGC TGACACAGGA ACGCGAGCTG 601 ATTTTCCAGG GTTTCGTACT GTTTCTCTGT TGGGCGGGTG CCGAGATGCA CTTTAGCCCC 661 GTCGCGATGT GAGAGGAGAG CACAGCGGTA TGACTTGGCG TTGTTCCGCA GAAAGTCTTG 721 CCATGACTCG CCTTCCAGGG GGCAGGAGTG GGTATGATGC CTGTCCAGCA TCTCGATTGG 781 CAGGGCATCG AGCAGGGCCC GCTTGTTCTT CACGTGCCAG TACAGGGTAG GCTGCTCAAC 841 TCCCAGCTTT TGAGCGAGTT TCCTTGTCGT CAGGCCTTCG ATACCGACTC CATTGAGTAA 901 TTCCAGGAGG CAGAGTTTAT GACTTTGGCT TCTTGTCCAG TCTAGACATG GGTAATTCGA 961 TGATCCCCCC TGGGGAGAGA GGTCGGTGAA TTCGGTCAAC GAAGGGAGTC GGACTGGCGG 1021 ACGTGCGCCT TCGGAGCCTG CCAGATGCCG AAACACCGCG TCGGGCCAGA CATGGCTCAA 1081 ACTACGACCA AGCCTGCTTC GGACCGGTCG TTCCAGGTCA GCTTAGCT // |
|
| Primer: pause site | RDB13919_A6B8b_2.seq |
|
>D03070A03_A6B8_1_Pause site_F09_18.ab1 1 GATTCCTTTC TCTCTCGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG AAGAGTTTAC 61 TCCCTATCAG TGATAGAGAA CGTATGCAGA CTTTACTCCC TATCAGTGAT AGAGAACGTA 121 TAAGGAGTTT ACTCCCTATC AGTGATAGAG AACGTATGAC CAGTTTACTC CCTATCAGTG 181 ATAGAGAACG TATCTACAGT TTACTCCCTA TCAGTGATAG AGAACGTATA TCCAGTTTAC 241 TCCCTATCAG TGATAGAGAA CGTATAAGCT TTGCTTATGT AAACCAGGGC GCCTATAAAA 301 GAGTGCTGAT TTTTTGAGTA AACTTCAATT CCACAACACT TTTGTCTTAT ACCAACTTTC 361 CGTACCACTT CCTACCCTCG TAAAGAATTC GCGGCCGCAC GCGTGCCACC ATGACATACT 421 TTCCTGAAGA GGTCGTCGAA CACATTTTTA GCTTCCTGCC TGCACAGAGA GATAGAAACA 481 CAGTGAGCCT GGTCTGCAAA GTGTGGTACG AGATCGAACG CCTGAGCCGG AGAGGAGTGT 541 TCGTCGGCAA CTGCTATGCT GTGAGAGCAG GCAGGGTCGC CGCTAGGTTT CCAAATGTGC 601 GCGCACTGAC CGTCAAGGGG AAACCCCACT TCGCCGACTT TAACCTGGTG CCCCCTGATT 661 GGGGAGGATA CGCCGGCCCT TGGATCGAGG CAGCCGCTCG CGGCTGTCAT GGACTGGAGG 721 AACTGCGCAT GAAGCGAATG GTGGTCTCTG ACGAAAGTCT GGAGCTGCTG GCTCGGAGCT 781 TCCCTAGGTT TCGCGCACTG GTGCTGATTT CTTGCGAAGG CTTCAGCACC GATGGACTGG 841 CAGCCGTGGC CTCCCACTGT AAGCTGCTGC GGGAGCTGGA CCTCCAGGAG AATGAAGTGG 901 AGGATAGAGG CCCCAGATGG CTGTCTTGCT TCCCAGACTC ATGTACCAGC CTGGGTGTCC 961 CTGAACTTTG CCTGCATCAA AGCGAAGTGA ATGCTGGGTC CCTGGAGCGG CTGGGTCTCA 1021 AGAAGCCCCC ACCTGAGTCT CTGCCGCTGA ACCCGGAGCG TGGAGCGTGA ACACTCTGGC 1081 TAGATCTGCT TGAGACCCCT TAACCTGCAG GATTCTGTGG GAAACCGC // |
|
| Primer: BGH_rev2 | RDB13919_A6B8c_2.seq |
|
>D03070A03_A6B8_1_BGH_rev2_G09_21.ab1 1 CTTCGTAGAG CACGGGGGAG GGGCAACAAC AGATGGCTGG CAACTAGAAG GCACAGGGTA 61 CCTCAGGCAC CGGGCTTGCG GGTCATGCAC CAGGTGCGCG GTCCTTCGGG CACCTCGACG 121 TCGGCGGTGA CGGTGAAGCC GAGCCGCTCG TAGAAGGGGA GGTTGCGGGG CGCGGAGGTC 181 TCCAGGAAGG CGGGCACCCC GGCGCGCTCG GCCGCCTCCA CTCCGGGGAG CACGACGGCG 241 CTGCCCAGAC CCTTGCCCTG GTGGTCGGGC GAGACGCCGA CGGTGGCCAG GAACCACGCG 301 GGCTCCTTGG GCCGGTGCGG CGCCAGGAGG CCTTCCATCT GTTGCTGCGC GGCCAGCCGG 361 GAACCGCTCA ACTCGGCCAT GCGCGGGCCG ATCTCGGCGA ACACCGCCCC CGCTTCGACG 421 CTCTCCGGCG TGGTCCAGAC CGCCACCGCG GCGCCGTCGT CCGCGACCCA CACCTTGCCG 481 ATGTCGAGCC CGACGCGCGT GAGGAAGAGT TCTTGCAGCT CGGTGACCCG CTCGATGTGG 541 CGGTCCCGGA TCGACGTGTG GCGCGTGGCG GGGTAGTCGG CGAACGCGGC GGCGAGGGTG 601 CGTACGGCCC TGGGGACGTC GTCGCGGGTG GCGAGGGCGC ACCGTGGGCT TGTACTTCGG 661 GTCATGGTAA GCTCCGGTGG ATCCCCCTTG // |
|
| Primer: M13R | RDB13919_A6B8d_2.seq |
|
>D03070A03_A6B8_1_M13R_F10_16.ab1 1 AAATCTCAAA TTACCCCTCA CTAAAGGGAC TAGTCCTGCA GGTTTAAAAC GAATTCGCCC 61 TTTGCTTTCT CTGACCAGCA TTCTCTCCCC TGGGCCTGTG CCGCTTTCTG TCTGCAGCTT 121 GTGGCCTGGG TCACCTCTAC GGCTGGCCCA GATCCTTCCC TGCCGCCTCC TTCAGGTTCC 181 GTCTTCCTCC ACTCCCTCTT CCCCTTGCTC TCTGCTGTGT TGCTGCCCAA GGATGCTCTT 241 TCCGGAGCAC TTCCTTCTCG GCGCTGCACC ACGTGATGTC CTCTGAGCGG ATCCTCCCCG 301 TGTCTGGGTC CTCTCCGGGC ATCTCTCCTC CCTCACCCAA CCCCATGCCG TCTTCACTCG 361 CTGGGTTCCC TTTTCCTTCT CCTTCTGGGG CCTGTGCCAT CTCTCGTTTC TTAGGATGGC 421 CTTCTCCGAC GGATGTCTCC CTTGCGTCCC GCCTCCCCTT CTTGTAGGCC TGCATCATCA 481 CCGTTTTTCT GGACAACCCC AAAGTACCCC GTCTCCCTGG CTTTAGCCAC CTCTCCATCC 541 TCTTGCTTTC TTTGCCTGGA CACCCCGTTC TCCTGTGGAT TCGGGTCACC TCTCACTCCT 601 TTCATTTGGG CAGCTCCCCT ACCCCCCTTA CCTCTCTAGT CTGTGCTAGC TCTTCCAGCC 661 CCCTGTCATG GCATCTTCCA GGGGTCCGAG AGCTCAGCTA GTCTTCTTCC TCCAACCCGG 721 GCCCCTATGT CCACTTCAGG ACAGCATGTT TGCTGCCTCC AGAGATCCCT GTGTCCCCGA 781 GCTGGGACCA ACCTT // |
|
| Primer: M13_-40 | RDB13919_A6B8e_2.seq |
|
>D03070A03_A6B8_1_M13(-40)_G10_19.ab1 1 CAGCCCGGAA TGTATACGAC TCACTATAGG GCGAATTGAA TTTAGCGGCC GCGAATTCGC 61 CCTTAGAGCA GAGCCAGGAA CCCCTGTAGG GAAGGGGCAG GAGAGCCAGG GGCATGAGAT 121 GGTGGACGAG GAAGGGGGAC AGGGAAGCCT GAGCGCCTCT CCTGGGCTTG CCAAGGACTC 181 AAACCCAGAA GCCCAGAGCA GGGCCTTAGG GAAGCGGGAC CCTGCTCTGG GCGGAGGAAT 241 ATGTCCCAGA TAGCACTGGG GACTCTTTAA GGAAAGAAGG ATGGAGAAAG AGAAAGGGAG 301 TAGAGGCGGC CACGACCTGG TGAACACCTA GGACGCACCA TTCTCACAAA GGGAGTTTTC 361 CACACGGACA CCCCCCTCCT CACCACAGCC CTGCCAGGAC GGGGCTGGCT ACTGGCCTTA 421 TCTCACAGGT AAAACTGACG CACGGAGGAA CAATATAAAT TGGGGACTAG AAAGGTGAAG 481 AGCCAAAGTT AGAACTCAGG ACCAACTTAT TCTGATTTTG TTTTTCCAAA CTGCTTCTCC 541 TCTTGGGAAG TGTAAGGAAG CTGCAGCACC AGGATCAGTG AAACGCACCA GACAGCCGCG 601 TCAGAGCAGC TCAGGTTCTG GGAGAGGGTA GCGCAGGGTG GCCACTGAGA ACCGGGCAGG 661 TCACGCATCC CCCCCTTCCC TCCCACCCCC TGCCAAGCTC TCCCTCCCAG GATCCTCTCT 721 GGCTCCATCG TAAGCAAACC TTAGAGGTTC TGGCAAGGAG AGAGATGGCT CCAGGAAATG 781 GGGGTGTGTC ACCAGATAAG GAATCTGCCT AACAGGAGGT GGGGGTTAGA CCCAATATCA 841 GGAGACTAGG AAAGGAGGAG GCCTAAGGAT GGGGCTTTTC TGTCACCAAT CCTGTCCCTA 901 GTAAAGCTTG GTACCCCATA GAGCCCACCG CATCCCCAGC ATGCCTGCTA TTGTCTTCCC 961 AATCCTCCCC CTTGCTGTCC TGCCCACCCA CCCCCAGAAT AAGAATGACA CCTACTCAGA 1021 CAATGCGATG CAATTTTCTC ATTTATTTAG GAAAGGACAG TGGGAGTGGC ACTTCCAGGT 1081 CCAGATGCAC TGGGAAGGTC AAACCACAGA ATGGCTTGCC AACCTTGAGG AAAGAGGCA // |
|
| Primer: TetOne oligo 2 | RDB13919_A6B8f_2.seq |
|
>D03070A03_A6B8_1_TetOne oligo 2_H10_22.ab1 1 CAGGTGCAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT 61 TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA 121 GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA 181 TTATGATCCT CTAGACATAT GCTGCAGAGA TCTTCACAGA ATCTTCACAA AGTTGGGAGC 241 ATCATCTCTT GCCCCTGCGG TGGTCCGATA CACATACAGT TTCTCCACCT TTGGCAGGTC 301 GCCATGATTT TCCTCCATTT CGTTAGACCC GTCCCGCTCA TTAATGACTT CCACGTTCAG 361 CATAGGCATT TTACTAGCCA GGACCTGACA GCCCTTCAGA GTCACATTGC AACTAGACAT 421 CCACAGGCTC CTCATTGTCT CGTATCTGGC AAAGTTTCCC AGCAGAGCGG CATCCCCGAA 481 AGGGCTGTCC CGGATTTCCA GTTTTCGCAG ATTCTTACAC CCCGTTCATC ACGTGCATCA 541 TGCCCTTATC GGAGTCTCCG GCAAATGCAT GGTTCACATC TCAGCTGCTT AGCATACTTG 601 CCGATGTACA TGAACACTTT TGTCTGTCAG CAGGCCGGAA ATGCTAAGCC TCTGAGATTC 661 CTTGC // |
|
Please visit Sequencing and PCR primers for primer information.
References
| original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. |
|---|---|
| reference | Devrekanli, A., Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes. Methods in Molecular Biology 1369: 257-278 (2016). PMID 26519318. |
| reference | Nishimura, K., Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Curr. Protoc. Cell Biol. \64: 20.9.1-16 (2014). PMID 25181302 . |
2018.12.11
GNP_filter3_RDBDEP_html_181012.pl