Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK243 (TetOne OsTIR1 AAVS1)

A donor vector for introducing Tet-OsTIR1 at the human AAVS1 locus.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] ＭＴＡの様式が異なります。お問い合わせください。
備考	書式の様式が異なります。お問い合わせください。 ＭＴＡの様式が異なります。お問い合わせください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13919_A6B8p1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: TetOne oligo 1 Region: SV40 p(A), Tet-On 3G Sequence file: RDB13919_A6B8g.seq
>D03070A03_A6B8_1_Tet One oligo1_E09_15.ab1     1 CTGCAGTGAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT    61 TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA   121 GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA   181 TTATGATCCT CTGGAGATTT ACCCGGGGAG CATGTCAAGG TCAAAATCGT CAAGAGCGTC   241 AGCAGGCAGC ATATCAAGGT CAAAGTCGTC AAGGGCATCG GCTGGGAGCA TGTCTAAGTC   301 AAAATCGTCA AGGGCGTCGG TCGGCCCGCC GCTTTCGCAC TTTAGCTGTT TCTCCAGGCC   361 ACATATGATT AGTTCCAGGC CGAAAAGGAA GGCAGGTTCG GCTCCCTGCC GGTCGAACAG   421 CTCAATTGCT TGTTTCAGAA GTGGGGGCAT AGAATCGGTG GTAGGTGTCT CTCTTTCCTC   481 TTTTGCTACT TGATGCTCCT GTTCCTCCAA TACGCAGCCC AGTGTAAAGT GGCCCACGGC   541 GGACAGAGCG TACAGTGCGT TCTCCAGGGA GAAGCCTTGC TGACACAGGA ACGCGAGCTG   601 ATTTTCCAGG GTTTCGTACT GTTTCTCTGT TGGGCGGGTG CCGAGATGCA CTTTAGCCCC   661 GTCGCGATGT GAGAGGAGAG CACAGCGGTA TGACTTGGCG TTGTTCCGCA GAAAGTCTTG   721 CCATGACTCG CCTTCCAGGG GGCAGGAGTG GGTATGATGC CTGTCCAGCA TCTCGATTGG   781 CAGGGCATCG AGCAGGGCCC GCTTGTTCTT CACGTGCCAG TACAGGGTAG GCTGCTCAAC   841 TCCCAGCTTT TGAGCGAGTT TCCTTGTCGT CAGGCCTTCG ATACCGACTC CATTGAGTAA   901 TTCCAGGAGG CAGAGTTTAT GACTTTGGCT TCTTGTCCAG TCTAGACATG GGTAATTCGA   961 TGATCCCCCC TGGGGAGAGA GGTCGGTGAA TTCGGTCAAC GAAGGGAGTC GGACTGGCGG  1021 ACGTGCGCCT TCGGAGCCTG CCAGATGCCG AAACACCGCG TCGGGCCAGA CATGGCTCAA 1081 ACTACGACCA AGCCTGCTTC GGACCGGTCG TTCCAGGTCA GCTTAGCT //
Primer: pause site Region: TRE3GS pro, OsTIR1 5' Sequence file: RDB13919_A6B8h.seq
>D03070A03_A6B8_1_Pause site_F09_18.ab1     1 GATTCCTTTC TCTCTCGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG AAGAGTTTAC    61 TCCCTATCAG TGATAGAGAA CGTATGCAGA CTTTACTCCC TATCAGTGAT AGAGAACGTA   121 TAAGGAGTTT ACTCCCTATC AGTGATAGAG AACGTATGAC CAGTTTACTC CCTATCAGTG   181 ATAGAGAACG TATCTACAGT TTACTCCCTA TCAGTGATAG AGAACGTATA TCCAGTTTAC   241 TCCCTATCAG TGATAGAGAA CGTATAAGCT TTGCTTATGT AAACCAGGGC GCCTATAAAA   301 GAGTGCTGAT TTTTTGAGTA AACTTCAATT CCACAACACT TTTGTCTTAT ACCAACTTTC   361 CGTACCACTT CCTACCCTCG TAAAGAATTC GCGGCCGCAC GCGTGCCACC ATGACATACT   421 TTCCTGAAGA GGTCGTCGAA CACATTTTTA GCTTCCTGCC TGCACAGAGA GATAGAAACA   481 CAGTGAGCCT GGTCTGCAAA GTGTGGTACG AGATCGAACG CCTGAGCCGG AGAGGAGTGT   541 TCGTCGGCAA CTGCTATGCT GTGAGAGCAG GCAGGGTCGC CGCTAGGTTT CCAAATGTGC   601 GCGCACTGAC CGTCAAGGGG AAACCCCACT TCGCCGACTT TAACCTGGTG CCCCCTGATT   661 GGGGAGGATA CGCCGGCCCT TGGATCGAGG CAGCCGCTCG CGGCTGTCAT GGACTGGAGG   721 AACTGCGCAT GAAGCGAATG GTGGTCTCTG ACGAAAGTCT GGAGCTGCTG GCTCGGAGCT   781 TCCCTAGGTT TCGCGCACTG GTGCTGATTT CTTGCGAAGG CTTCAGCACC GATGGACTGG   841 CAGCCGTGGC CTCCCACTGT AAGCTGCTGC GGGAGCTGGA CCTCCAGGAG AATGAAGTGG   901 AGGATAGAGG CCCCAGATGG CTGTCTTGCT TCCCAGACTC ATGTACCAGC CTGGGTGTCC   961 CTGAACTTTG CCTGCATCAA AGCGAAGTGA ATGCTGGGTC CCTGGAGCGG CTGGGTCTCA  1021 AGAAGCCCCC ACCTGAGTCT CTGCCGCTGA ACCCGGAGCG TGGAGCGTGA ACACTCTGGC 1081 TAGATCTGCT TGAGACCCCT TAACCTGCAG GATTCTGTGG GAAACCGC //
Primer: BGH_rev2 Region: BGH p(A), PuroR Sequence file: RDB13919_A6B8i.seq
>D03070A03_A6B8_1_BGH_rev2_G09_21.ab1     1 CTTCGTAGAG CACGGGGGAG GGGCAACAAC AGATGGCTGG CAACTAGAAG GCACAGGGTA    61 CCTCAGGCAC CGGGCTTGCG GGTCATGCAC CAGGTGCGCG GTCCTTCGGG CACCTCGACG   121 TCGGCGGTGA CGGTGAAGCC GAGCCGCTCG TAGAAGGGGA GGTTGCGGGG CGCGGAGGTC   181 TCCAGGAAGG CGGGCACCCC GGCGCGCTCG GCCGCCTCCA CTCCGGGGAG CACGACGGCG   241 CTGCCCAGAC CCTTGCCCTG GTGGTCGGGC GAGACGCCGA CGGTGGCCAG GAACCACGCG   301 GGCTCCTTGG GCCGGTGCGG CGCCAGGAGG CCTTCCATCT GTTGCTGCGC GGCCAGCCGG   361 GAACCGCTCA ACTCGGCCAT GCGCGGGCCG ATCTCGGCGA ACACCGCCCC CGCTTCGACG   421 CTCTCCGGCG TGGTCCAGAC CGCCACCGCG GCGCCGTCGT CCGCGACCCA CACCTTGCCG   481 ATGTCGAGCC CGACGCGCGT GAGGAAGAGT TCTTGCAGCT CGGTGACCCG CTCGATGTGG   541 CGGTCCCGGA TCGACGTGTG GCGCGTGGCG GGGTAGTCGG CGAACGCGGC GGCGAGGGTG   601 CGTACGGCCC TGGGGACGTC GTCGCGGGTG GCGAGGGCGC ACCGTGGGCT TGTACTTCGG 661 GTCATGGTAA GCTCCGGTGG ATCCCCCTTG //
Primer: M13R Region: AAVS1 arm 1 Sequence file: RDB13919_A6B8j.seq
>D03070A03_A6B8_1_M13R_F10_16.ab1     1 AAATCTCAAA TTACCCCTCA CTAAAGGGAC TAGTCCTGCA GGTTTAAAAC GAATTCGCCC    61 TTTGCTTTCT CTGACCAGCA TTCTCTCCCC TGGGCCTGTG CCGCTTTCTG TCTGCAGCTT   121 GTGGCCTGGG TCACCTCTAC GGCTGGCCCA GATCCTTCCC TGCCGCCTCC TTCAGGTTCC   181 GTCTTCCTCC ACTCCCTCTT CCCCTTGCTC TCTGCTGTGT TGCTGCCCAA GGATGCTCTT   241 TCCGGAGCAC TTCCTTCTCG GCGCTGCACC ACGTGATGTC CTCTGAGCGG ATCCTCCCCG   301 TGTCTGGGTC CTCTCCGGGC ATCTCTCCTC CCTCACCCAA CCCCATGCCG TCTTCACTCG   361 CTGGGTTCCC TTTTCCTTCT CCTTCTGGGG CCTGTGCCAT CTCTCGTTTC TTAGGATGGC   421 CTTCTCCGAC GGATGTCTCC CTTGCGTCCC GCCTCCCCTT CTTGTAGGCC TGCATCATCA   481 CCGTTTTTCT GGACAACCCC AAAGTACCCC GTCTCCCTGG CTTTAGCCAC CTCTCCATCC   541 TCTTGCTTTC TTTGCCTGGA CACCCCGTTC TCCTGTGGAT TCGGGTCACC TCTCACTCCT   601 TTCATTTGGG CAGCTCCCCT ACCCCCCTTA CCTCTCTAGT CTGTGCTAGC TCTTCCAGCC   661 CCCTGTCATG GCATCTTCCA GGGGTCCGAG AGCTCAGCTA GTCTTCTTCC TCCAACCCGG   721 GCCCCTATGT CCACTTCAGG ACAGCATGTT TGCTGCCTCC AGAGATCCCT GTGTCCCCGA 781 GCTGGGACCA ACCTT //
Primer: M13_-40 Region: T7 pro, AAVS1 arm 2 Sequence file: RDB13919_A6B8k.seq
>D03070A03_A6B8_1_M13(-40)_G10_19.ab1     1 CAGCCCGGAA TGTATACGAC TCACTATAGG GCGAATTGAA TTTAGCGGCC GCGAATTCGC    61 CCTTAGAGCA GAGCCAGGAA CCCCTGTAGG GAAGGGGCAG GAGAGCCAGG GGCATGAGAT   121 GGTGGACGAG GAAGGGGGAC AGGGAAGCCT GAGCGCCTCT CCTGGGCTTG CCAAGGACTC   181 AAACCCAGAA GCCCAGAGCA GGGCCTTAGG GAAGCGGGAC CCTGCTCTGG GCGGAGGAAT   241 ATGTCCCAGA TAGCACTGGG GACTCTTTAA GGAAAGAAGG ATGGAGAAAG AGAAAGGGAG   301 TAGAGGCGGC CACGACCTGG TGAACACCTA GGACGCACCA TTCTCACAAA GGGAGTTTTC   361 CACACGGACA CCCCCCTCCT CACCACAGCC CTGCCAGGAC GGGGCTGGCT ACTGGCCTTA   421 TCTCACAGGT AAAACTGACG CACGGAGGAA CAATATAAAT TGGGGACTAG AAAGGTGAAG   481 AGCCAAAGTT AGAACTCAGG ACCAACTTAT TCTGATTTTG TTTTTCCAAA CTGCTTCTCC   541 TCTTGGGAAG TGTAAGGAAG CTGCAGCACC AGGATCAGTG AAACGCACCA GACAGCCGCG   601 TCAGAGCAGC TCAGGTTCTG GGAGAGGGTA GCGCAGGGTG GCCACTGAGA ACCGGGCAGG   661 TCACGCATCC CCCCCTTCCC TCCCACCCCC TGCCAAGCTC TCCCTCCCAG GATCCTCTCT   721 GGCTCCATCG TAAGCAAACC TTAGAGGTTC TGGCAAGGAG AGAGATGGCT CCAGGAAATG   781 GGGGTGTGTC ACCAGATAAG GAATCTGCCT AACAGGAGGT GGGGGTTAGA CCCAATATCA   841 GGAGACTAGG AAAGGAGGAG GCCTAAGGAT GGGGCTTTTC TGTCACCAAT CCTGTCCCTA   901 GTAAAGCTTG GTACCCCATA GAGCCCACCG CATCCCCAGC ATGCCTGCTA TTGTCTTCCC   961 AATCCTCCCC CTTGCTGTCC TGCCCACCCA CCCCCAGAAT AAGAATGACA CCTACTCAGA  1021 CAATGCGATG CAATTTTCTC ATTTATTTAG GAAAGGACAG TGGGAGTGGC ACTTCCAGGT 1081 CCAGATGCAC TGGGAAGGTC AAACCACAGA ATGGCTTGCC AACCTTGAGG AAAGAGGCA //
Primer: TetOne oligo 2 Region: OsTIR1 3' Sequence file: RDB13919_A6B8l.seq
>D03070A03_A6B8_1_TetOne oligo 2_H10_22.ab1     1 CAGGTGCAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT    61 TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA   121 GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA   181 TTATGATCCT CTAGACATAT GCTGCAGAGA TCTTCACAGA ATCTTCACAA AGTTGGGAGC   241 ATCATCTCTT GCCCCTGCGG TGGTCCGATA CACATACAGT TTCTCCACCT TTGGCAGGTC   301 GCCATGATTT TCCTCCATTT CGTTAGACCC GTCCCGCTCA TTAATGACTT CCACGTTCAG   361 CATAGGCATT TTACTAGCCA GGACCTGACA GCCCTTCAGA GTCACATTGC AACTAGACAT   421 CCACAGGCTC CTCATTGTCT CGTATCTGGC AAAGTTTCCC AGCAGAGCGG CATCCCCGAA   481 AGGGCTGTCC CGGATTTCCA GTTTTCGCAG ATTCTTACAC CCCGTTCATC ACGTGCATCA   541 TGCCCTTATC GGAGTCTCCG GCAAATGCAT GGTTCACATC TCAGCTGCTT AGCATACTTG   601 CCGATGTACA TGAACACTTT TGTCTGTCAG CAGGCCGGAA ATGCTAAGCC TCTGAGATTC 661 CTTGC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.02.09

GNP_filter3_RDBDEP_html_220112.pl