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pMK411 (#RDB18364)

TOL2 expression vector of OsTIR1(F74G) and mAID-EGFP-Nluc marker.

Alternative name OsTIR1(F74G)mAID-EGFP-Nluc
Clone info. TOL2 expression vector of OsTIR1(F74G) and mAID-EGFP-Nluc marker, see Fig. 6 of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone pT2AL200R175-CAGGS-EGFP (plasmid)
Selectable markers Ampicillin
Gene/insert name Oryza sativa OsTIR1 cDNA Arabidopsis thaliana IAA17 cDNA Aequorea victoria GFP (EGFP) cDNA
Depositor|Developer Kanemaki, Masato |
 
Sequence (full) RDB18364zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Koichi Kawakami of the National Institute of Genetics (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004))is requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
MTAに書く付加的使用条件:
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。利用者は、研究成果の公表にあたって国立遺伝学研究所 川上浩一博士が指定する文献 (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004)) の引用を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18364 pMK411 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMK411 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18364).

Reference section:

Yesbolatova, A., Saito, Y., Kitamoto, N., Makino-Itou, H., Ajima, R., Nakano, R., Nakaoka, H., Fukui, K., Gamo, K., Tominari, Y., Takeuchi, H., Saga, Y., Hayashi, K., Kanemaki, M.T., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18364_B0Gfp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: reverse-2 (Pr0100)
Region: Tol2L200,CMV en,chicken beta-actin pro,
Sequence file: RDB18364_B0Gfa.seq check
>D07105D5_B0Gf_3_reverse-2_A07_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNCGCNNN NNNNNCTCAC TAAAGGGAAC AAAAGCTGGA
   61 GCTCGGATCT GCTGGGCTNG NNNAAGGTAG GGGGTCAAGA ACCAGAGGTG TAAAGTACTT
  121 GAGTAATTTT ACTTGATTAC TGTACTTAAG TATTATTTTT GGGGATTTTT ACTTTACTTG
  181 AGTACAATTA AAAATCAATA CTTTTACTTT TACTTAATTA CATTTTTTTA GAAAAAAAAG
  241 TACTTTTTAC TCCTTACAAT TTTATTTACA GTCAAAAAGT ACTTATTTTT TGGAGATCAC
  301 TTGGGCCCTC TAGATGCATG CTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT
  361 TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA
  421 TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT
  481 TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA
  541 AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT
  601 CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC
  661 TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTCGAGG TGAGCCCCAC
  721 GTTCTGCTTC ACTCTCCCCA TCTCCCCCCC CTCCCCACCC CCAATTTTGT ATTTATTTAT
781 TTTTNNATTA TTTTGTGCAG CGATGGGGGC GNNGGGGGGG GNNNNNNNNN N
//
Primer: pCAG-F_V3 (Pr0495)
Region: chimeric intron, OsTIR1(F74G) 5'
Sequence file: RDB18364_B0Gfb.seq check
>D07105D5_B0Gf_3_pCAG-F_V3_B07_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTNNNNNNNN NNNNNNGCAN NGCGGNNNNN NNCTTCTGGC
   61 GTGTGACCGG CGGCTCTANA NNNTCTGCNN NNCNTGTTCA TGCCTTCTTC TTTTTCCTAC
  121 AGCTCCTGGG CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCGAGC
  181 TCGGTACCTC GCGAATGCAT CTAGATAGCG CTGCCACCAT GACATACTTT CCTGAAGAGG
  241 TCGTCGAACA CATTTTTAGC TTCCTGCCTG CACAGAGAGA TAGAAACACA GTGAGCCTGG
  301 TCTGCAAAGT GTGGTACGAG ATCGAACGCC TGAGCCGGAG AGGAGTGTTC GTCGGCAACT
  361 GCTATGCTGT GAGAGCAGGC AGGGTCGCCG CTAGGTTTCC AAATGTGCGC GCACTGACCG
  421 TCAAGGGGAA ACCCCACGGC GCCGACTTTA ACCTGGTGCC CCCTGATTGG GGAGGATACG
  481 CCGGCCCTTG GATCGAGGCA GCCGCTCGCG GCTGTCATGG ACTGGAGGAA CTGCGCATGA
  541 AGCGAATGGT GGTCTCTGAC GAAAGTCTGG AGCTGCTGGC TCGGAGCTTC CCTAGGTTTC
  601 GCGCACTGGT GCTGATTTCT TGCGAAGGCT TCAGCACCGA TGGACTGGNN NNCGTGGCCT
  661 CCCACTGTAA GCTGCTGCGG GAGCTGGACC TCCAGGAGAA TGAANTGGAG GATAGAGGCC
  721 CCAGATGGCT GTCTTGCTTN CCAGACTCAT GTACCAGCCT GGTGTCCCTG AACTTTGCCT
  781 GCNTCNANNN CGANNTGAAT GCTGGGTCCC TGNANCGGNT GGTCTNNNNA AGCCCCAACC
  841 TGAGGNCTCT GCNGNNTNAN CCGGANCGNN NNNGTGNNNA CTNTGGCNNA NNANNCTNNN
  901 NGAGAANCCC TANCCTGNNN GANCTNGGAA NNCGNNNANN NTGANANANG ANTTTCNNNA
  961 NNNNAATNCN NNNTTTTANN NTGNNNNTCT GCCCNNGNNN NNANNNNNNN NAANGNNNAN
 1021 GNNNNNNGNC NNNGATTCNN NGGNNNGNTN NNNNNNNNGN NNNCNNGANN NTTNANNNTN
1081 NCCCNNNNNN NNNN
//
Primer: YFP_R (Pr0127)
Region: EGFP 5', mAID, P2A, OsTIR1(F74G) 3'
Sequence file: RDB18364_B0Gfc.seq check
>D07105D5_B0Gf_3_YFP_R_C07_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC NNNNNNTGTG GCCGTTTACG TCGCCGTCCA
   61 GCTCGACCAG GATGGNCNNN NNNNNGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGCTAG
  121 CTTTATACAT CCTCAAATCG ATTTTCCTCA AGTACGGTGC TCCGTCCATT GATACCTTCA
  181 CGAACGCCGC CGCCTCCGGG CCACCGCTTG ATTTTTGGCA GGAAACCATC ACGTTCTTCC
  241 GGTATGATCT CACCGGTGGC CATCCCACAA CTTGTGCCTT GGCCGGAGGT TTGGCTGGAT
  301 CTTTAGGACA AGCACTCTTC TCCTTAGATC TAGGTCCAGG GTTCTCCTCC ACGTCTCCAG
  361 CCTGCTTCAG CAGGCTGAAG TTAGTAGCTC CGCTTCCTGA TCTCAGAATC TTCACAAAGT
  421 TGGGAGCATC ATCTCTTGCC CCTGCGGTGG TCCGATACAC ATACAGTTTC TCCACCTTTG
  481 GCAGGTCGCC ATGATTTTCC TCCATTTCGT TAGACCCGTC CCGCTCATTA ATGACTTCCA
  541 CGTTCAGCAT AGGCATTTTA CTAGCCAGGA CCTGACAGCC CTTCAGAGTC ACATTGCAAC
  601 TAGACATCCA CAGGCTCCTC ATTGTCTCGT ATCTGGCAAA GTTTCCCAGC AGAGCGGCAT
  661 CCCCGAAAGG GCTGTCCCGG ATTTCCAGTT TTCGCAGATT CTTACACCCG TTCATCACGT
  721 GCATCATGCC CTTATCGGAN TCTCCGGCAA ATGCAATGCT CAGCATCTCC AGCTGCTTAN
  781 NNTACNTTGC CGATGTACAT GAACACTTTG TCTGTCANCA GGNCNGAAAT GNTCANCCTC
  841 TGGANTCCCT TGCACTCTCT CACGATAGCT CCAAACCCTT CATCCAGTGG NTGGNNTGNG
  901 ACCACGTCGG GTTTGNCTGG NTCNNNAANA NACANCNNAA ANCNGGTGAA ATTANGGGNA
961 GTTCNNGNNN ANNGNNNCCA NTGNANNANN NNNNNATCTG ATGANANAAN NNNNNNNNN
//
Primer: EGFP-C2 (Pr0402)
Region: EGFP 3', Linker, Nluc,
Sequence file: RDB18364_B0Gfd.seq check
>D07105D5_B0Gf_3_EGFP-C2_D07_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNANNNN NNNNCNNNTG GTCCTGCTGG ANTTCGTGAC CGCCGCCGGG
   61 ATCACTCTCG GCATGNNNNA NCTGTACAAG GGTGCAGGCG CCCAAGCTTC GAATTCTGCA
  121 GTCGACATGG TCTTCACACT CGAAGATTTC GTTGGGGACT GGCGACAGAC AGCCGGCTAC
  181 AACCTGGACC AAGTCCTTGA ACAGGGAGGT GTGTCCAGTT TGTTTCAGAA TCTCGGGGTG
  241 TCCGTAACTC CGATCCAAAG GATTGTCCTG AGCGGTGAAA ATGGGCTGAA GATCGACATC
  301 CATGTCATCA TCCCGTATGA AGGTCTGAGC GGCGACCAAA TGGGCCAGAT CGAAAAAATT
  361 TTTAAGGTGG TGTACCCTGT GGATGATCAT CACTTTAAGG TGATCCTGCA CTATGGCACA
  421 CTGGTAATCG ACGGGGTTAC GCCGAACATG ATCGACTATT TCGGACGGCC GTATGAAGGC
  481 ATCGCCGTGT TCGACGGCAA AAAGATCACT GTAACAGGGA CCCTGTGGAA CGGCAACAAA
  541 ATTATCGACG AGCGCCTGAT CAACCCCGAC GGCTCCCTGC TGTTCCGAGT AACCATCAAC
  601 GGAGTGACCG GCTGGCGGCT GTGCGAACGC ATTCTGGCGT AAGGATCCAC CGGATCTAGA
  661 TAACTGATCA TAATCAGCCA TACCACATTT GNNNNNNNTT TANNNNNNTT AAAAAACCTC
  721 CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTNNNNNN NTAACTTGTT
  781 TATTGCAGCT TATAATGGTT ACNAAATAAA GCAATAGCAT CACNANTTTC ACNANTAAAG
  841 CATTTTTTTC ACTGCNTTCT ANNTGTGGTT NGTNNNAANC TCATCAATGT ATCTTNNNGC
  901 GTCGATCATA NTNCNATAAC CCTTANNATA NCTTCGNATA ATGNATGCTA TACNANNTTA
  961 TTANNTCTGA AGANNANNTT NNGNCCANNC NANCTTNNNA TNNCGANCTC GAAATTCTNN
 1021 NNGGTNNGGN NNGNGCTTTT CNNNNGNANN CNNNNNCANN CNNNTNNNNN NNNCNNNNNN
1081 NNNN
//
Primer: PGKpro_R (Pr0793)
Region: PGKpro, loxP, SV40 pA, Nluc
Sequence file: RDB18364_B0Gfe.seq check
>D07105D5_B0Gf_3_PGKpro_R_E07_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNGANN NNNNAGGCCN CTTGTGTAGC
   61 GCCNAGTGCC CAGCGGGGCT GCTAANGCGC ATGCTCCAGA CTGCCTTGGG AAAAGCGCCT
  121 CCCCTACCCG GTAGAATTTC GAGGTCGAGA TCCTAAGCTT GGCTGGACGT AAACTCCTCT
  181 TCAGACCTAA TAACTTCGTA TAGCATACAT TATACGAAGT TATATTAAGG GTTATTGAAT
  241 ATGATCGACG CGTTAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA
  301 AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC
  361 TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG
  421 GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC TGATTATGAT
  481 CAGTTATCTA GATCCGGTGG ATCCTTACGC CAGAATGCGT TCGCACAGCC GCCAGCCGGT
  541 CACTCCGTTG ATGGTTACTC GGAACAGCAG GGAGCCGTCG GGGTTGATCA GGCGCTCGTC
  601 GATAATTTTG TTGCCGTTCC ACAGGGTCCC TGTTACAGTG ATCTTTTTGC CGTCGAACAC
  661 GGCGATGCCT TCNTACGGCC GTCCGAAATA GTCNATCATG TTCGGCGTAA CCCCGTCGAT
  721 TACCAGTGTG CCATANNNNN NGATCACCTT AAAGTGATGA TCATCCNNNN NGTACACCAN
  781 CCTTNAAANT TTTTTCNATC TGGCCNNTTT GNTCGCCGCT CAGACCTTCN TACGGGANGA
  841 NGACNTGGAT GTCNATCTTN NNCCCATTTT CNNCGNTCAN GACAATCCTT TNNNTNNNNN
901 NNACNNANAC CCCGANANTT CTGAAACAAN TGNANACNNC TNCCTGNNNN NNNNNN
//
Primer: PGKpro_F (Pr0249)
Region: HygR
Sequence file: RDB18364_B0Gff.seq check
>D07105D5_B0Gf_3_PGKpro_F_F07_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTCT CCGGGCCTTT CGNNCTGCAT CCNTCTAGAT
   61 CTCGAGCAGC TGAAGCTTAC CGCTAGCATG GATAGATCCG GAAAGCCTGA ACTCACCGCG
  121 ACGTCTGTCG AGAAGTTTCT GATCGAAAAG TTCGACAGCG TCTCCGACCT GATGCAGCTC
  181 TCGGAGGGCG AAGAATCTCG TGCTTTCAGC TTCGATGTAG GAGGGCGTGG ATATGTCCTG
  241 CGGGTAAATA GCTGCGCCGA TGGTTTCTAC AAAGATCGTT ATGTTTATCG GCACTTTGCA
  301 TCGGCCGCGC TCCCGATTCC GGAAGTGCTT GACATTGGGG AATTCAGCGA GAGCCTGACC
  361 TATTGCATCT CCCGCCGTGC ACAGGGTGTC ACGTTGCAAG ACCTGCCTGA AACCGAACTG
  421 CCCGCTGTTC TGCAGCCGGT CGCGGAGGCC ATGGATGCGA TCGCTGCGGC CGATCTTAGC
  481 CAGACGAGCG GGTTCGGCCC ATTCGGACCG CAAGGAATCG GTCAATACAC TACATGGCGT
  541 GATTTCATAT GCGCGATTGC TGATCCCCAT GTGTATCACT GGCAAACTGT GATGGACGAC
  601 ACCGTCAGTG CGTCCGTCGC GCAGGCTCTC GATGAGCTGA TGCTTTGGGC CGAGGACTGC
  661 CCCGAAGTCC GGCACCTCGT GCACGCGGAT TTCGGCTCCN ACAATGTCCT GACGGACAAT
  721 GGCCGCATAA CAGCGGTCAT TGACTGGAGC GAGGCGATGT TCGGGGANTC CCAATACGAG
  781 GTCGCCAACA TCTTCTTCTG GANGNCGTGG TTGGCTTGTA TGGAGCAGCA GANGCGCTAC
  841 TTCGAGCGGA NGCATCCGGA GCTTGNANGA TCGCCGCGGC TNNNGGCGTA TATGCTCCGC
  901 NTTGGTCTTG ANCNACTCTA TCANANNTTG GTTGANGGNN ATTTCGATGA NGCANNTTNN
  961 NNNNNNTCNN NGNNANGCNN NCGTCCNATN NNNANNCNNG GNNNGNCNGG CGTANNNAAA
1021 TCNNNNCANA ANCNNGGNNN TCNNGNANNG NTNGNNNNNN NNNN
//
Primer: pUC-M13_-100 (Pr0310)
Region: Tol2R175, loxP, mouse PGK ter
Sequence file: RDB18364_B0Gfg.seq check
>D07105D5_B0Gf_3_pUC-M13_-100_G07_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCCNGGGT TTTCCCNGTC NCGACGTTGN
   61 NNNNNGACGG CCAGTGAGCG CGCGNAATAC GACTCACTAT AGGGCGAATT GGGTACCGGC
  121 ATATGGTTCT TGACAGAGGT GTAAAAAGTA CTCAAAAATT TTACTCAAGT GAAAGTACAA
  181 GTACTTAGGG AAAATTTTAC TCAATTAAAA GTAAAAGTAT CTGGCTAGAA TCTTACTTGA
  241 GTAAAAGTAA AAAAGTACTC CATTAAAATT GTACTTGAGT ATTAAGGAAG TAAAAGTAAA
  301 AGCAAGAAAG ATCTTAGTGA ACCTCTTCGA GGGACCTAAT AACTTCGTAT AGCATACATT
  361 ATACGAAGTT ATATTAAGGG TTCCGGATCT CGACCAGCTT CTGATGGAAT TAGAACTTGG
  421 CAAAACAATA CTGAGAATGA AGTGTATGTG GAACAGAGGC TGCTGATCTC GTTCTTCAGG
  481 CTATGAAACT GACACATTTG GAAACCACAG TACTTAGAAC CACAAAGTGG GAATCAAGAG
  541 AAAAACAATG ATCCCACNAN AGATCTATAG ATCTATAGAT CATGAGTGGG AGGAATGAGC
  601 TGGCCCTTAA TTTGGTTTTG CTTGTTTAAA TTATGATATC CAACTATGAA ACATTATCAT
  661 AAAGCAATAG TAAAGAGCCT TCAGTAAAGA GCAGGCATTT ATCTAATCCC ACCCCACCCC
  721 CACCCCCGTA GCTCCNATCC TTCCATTCAA AATGTANGTA CTCTGTTCTC ACCCTTCTNN
  781 ACAAAGTANG ACAGGAAAAA CTTCCATTTT ANTGNACATC TTTATTGNTT AATAGATCAT
  841 CAATTTCTGC ATCCCGGGGA NCTGANATCN TCGATGCNTG GGGGTCGTGC GCTCCTTTCG
  901 GTCGGGCGCT GCGGGGTCNN GGGGCGGGCG GNTANNNNAT TCCNTNGCCN TCNNANNANN
  961 GCTGGGGCGT NNGTTTNCAC TATCNNNNAG TNNNNNNNCN NAGCCNTCGG NCCNNANNNN
 1021 NNCNCTTCNN NNGNNNNANT NNNGNANNNC NNNNNNNNCC NGNNNCCNGN ANNNNNNNNN
1081 ANNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]