TOL2 expression vector of OsTIR1(F74G) and mAID-EGFP-Nluc marker.
Alternative name | OsTIR1(F74G)mAID-EGFP-Nluc |
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Clone info. | TOL2 expression vector of OsTIR1(F74G) and mAID-EGFP-Nluc marker, see Fig. 6 of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020. |
Vector backbone | pT2AL200R175-CAGGS-EGFP (plasmid) |
Selectable markers | Ampicillin |
Gene/insert name | Oryza sativa OsTIR1 cDNA Arabidopsis thaliana IAA17 cDNA Aequorea victoria GFP (EGFP) cDNA |
Depositor|Developer | Kanemaki, Masato | |
Sequence (full) ![]() |
RDB18364zzk01.seq provided by the depositor |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)). Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation. The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Koichi Kawakami of the National Institute of Genetics (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004))is requested. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。 MTAに書く付加的使用条件: 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。 Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。利用者は、研究成果の公表にあたって国立遺伝学研究所 川上浩一博士が指定する文献 (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004)) の引用を必要とする。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB18364 | pMK411 | DNA solution |
Materials & Methods section:
The pMK411 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18364). |
Reference section:
Yesbolatova, A., Saito, Y., Kitamoto, N., Makino-Itou, H., Ajima, R., Nakano, R., Nakaoka, H., Fukui, K., Gamo, K., Tominari, Y., Takeuchi, H., Saga, Y., Hayashi, K., Kanemaki, M.T., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB18364_B0Gfp1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: reverse-2 (Pr0100) Region: Tol2L200,CMV en,chicken beta-actin pro, Sequence file: RDB18364_B0Gfa.seq ![]() |
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>D07105D5_B0Gf_3_reverse-2_A07_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNCGCNNN NNNNNCTCAC TAAAGGGAAC AAAAGCTGGA 61 GCTCGGATCT GCTGGGCTNG NNNAAGGTAG GGGGTCAAGA ACCAGAGGTG TAAAGTACTT 121 GAGTAATTTT ACTTGATTAC TGTACTTAAG TATTATTTTT GGGGATTTTT ACTTTACTTG 181 AGTACAATTA AAAATCAATA CTTTTACTTT TACTTAATTA CATTTTTTTA GAAAAAAAAG 241 TACTTTTTAC TCCTTACAAT TTTATTTACA GTCAAAAAGT ACTTATTTTT TGGAGATCAC 301 TTGGGCCCTC TAGATGCATG CTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT 361 TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA 421 TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT 481 TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA 541 AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT 601 CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC 661 TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTCGAGG TGAGCCCCAC 721 GTTCTGCTTC ACTCTCCCCA TCTCCCCCCC CTCCCCACCC CCAATTTTGT ATTTATTTAT 781 TTTTNNATTA TTTTGTGCAG CGATGGGGGC GNNGGGGGGG GNNNNNNNNN N // |
Primer: pCAG-F_V3 (Pr0495) Region: chimeric intron, OsTIR1(F74G) 5' Sequence file: RDB18364_B0Gfb.seq ![]() |
>D07105D5_B0Gf_3_pCAG-F_V3_B07_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NTNNNNNNNN NNNNNNGCAN NGCGGNNNNN NNCTTCTGGC 61 GTGTGACCGG CGGCTCTANA NNNTCTGCNN NNCNTGTTCA TGCCTTCTTC TTTTTCCTAC 121 AGCTCCTGGG CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCGAGC 181 TCGGTACCTC GCGAATGCAT CTAGATAGCG CTGCCACCAT GACATACTTT CCTGAAGAGG 241 TCGTCGAACA CATTTTTAGC TTCCTGCCTG CACAGAGAGA TAGAAACACA GTGAGCCTGG 301 TCTGCAAAGT GTGGTACGAG ATCGAACGCC TGAGCCGGAG AGGAGTGTTC GTCGGCAACT 361 GCTATGCTGT GAGAGCAGGC AGGGTCGCCG CTAGGTTTCC AAATGTGCGC GCACTGACCG 421 TCAAGGGGAA ACCCCACGGC GCCGACTTTA ACCTGGTGCC CCCTGATTGG GGAGGATACG 481 CCGGCCCTTG GATCGAGGCA GCCGCTCGCG GCTGTCATGG ACTGGAGGAA CTGCGCATGA 541 AGCGAATGGT GGTCTCTGAC GAAAGTCTGG AGCTGCTGGC TCGGAGCTTC CCTAGGTTTC 601 GCGCACTGGT GCTGATTTCT TGCGAAGGCT TCAGCACCGA TGGACTGGNN NNCGTGGCCT 661 CCCACTGTAA GCTGCTGCGG GAGCTGGACC TCCAGGAGAA TGAANTGGAG GATAGAGGCC 721 CCAGATGGCT GTCTTGCTTN CCAGACTCAT GTACCAGCCT GGTGTCCCTG AACTTTGCCT 781 GCNTCNANNN CGANNTGAAT GCTGGGTCCC TGNANCGGNT GGTCTNNNNA AGCCCCAACC 841 TGAGGNCTCT GCNGNNTNAN CCGGANCGNN NNNGTGNNNA CTNTGGCNNA NNANNCTNNN 901 NGAGAANCCC TANCCTGNNN GANCTNGGAA NNCGNNNANN NTGANANANG ANTTTCNNNA 961 NNNNAATNCN NNNTTTTANN NTGNNNNTCT GCCCNNGNNN NNANNNNNNN NAANGNNNAN 1021 GNNNNNNGNC NNNGATTCNN NGGNNNGNTN NNNNNNNNGN NNNCNNGANN NTTNANNNTN 1081 NCCCNNNNNN NNNN // |
Primer: YFP_R (Pr0127) Region: EGFP 5', mAID, P2A, OsTIR1(F74G) 3' Sequence file: RDB18364_B0Gfc.seq ![]() |
>D07105D5_B0Gf_3_YFP_R_C07_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC NNNNNNTGTG GCCGTTTACG TCGCCGTCCA 61 GCTCGACCAG GATGGNCNNN NNNNNGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGCTAG 121 CTTTATACAT CCTCAAATCG ATTTTCCTCA AGTACGGTGC TCCGTCCATT GATACCTTCA 181 CGAACGCCGC CGCCTCCGGG CCACCGCTTG ATTTTTGGCA GGAAACCATC ACGTTCTTCC 241 GGTATGATCT CACCGGTGGC CATCCCACAA CTTGTGCCTT GGCCGGAGGT TTGGCTGGAT 301 CTTTAGGACA AGCACTCTTC TCCTTAGATC TAGGTCCAGG GTTCTCCTCC ACGTCTCCAG 361 CCTGCTTCAG CAGGCTGAAG TTAGTAGCTC CGCTTCCTGA TCTCAGAATC TTCACAAAGT 421 TGGGAGCATC ATCTCTTGCC CCTGCGGTGG TCCGATACAC ATACAGTTTC TCCACCTTTG 481 GCAGGTCGCC ATGATTTTCC TCCATTTCGT TAGACCCGTC CCGCTCATTA ATGACTTCCA 541 CGTTCAGCAT AGGCATTTTA CTAGCCAGGA CCTGACAGCC CTTCAGAGTC ACATTGCAAC 601 TAGACATCCA CAGGCTCCTC ATTGTCTCGT ATCTGGCAAA GTTTCCCAGC AGAGCGGCAT 661 CCCCGAAAGG GCTGTCCCGG ATTTCCAGTT TTCGCAGATT CTTACACCCG TTCATCACGT 721 GCATCATGCC CTTATCGGAN TCTCCGGCAA ATGCAATGCT CAGCATCTCC AGCTGCTTAN 781 NNTACNTTGC CGATGTACAT GAACACTTTG TCTGTCANCA GGNCNGAAAT GNTCANCCTC 841 TGGANTCCCT TGCACTCTCT CACGATAGCT CCAAACCCTT CATCCAGTGG NTGGNNTGNG 901 ACCACGTCGG GTTTGNCTGG NTCNNNAANA NACANCNNAA ANCNGGTGAA ATTANGGGNA 961 GTTCNNGNNN ANNGNNNCCA NTGNANNANN NNNNNATCTG ATGANANAAN NNNNNNNNN // |
Primer: EGFP-C2 (Pr0402) Region: EGFP 3', Linker, Nluc, Sequence file: RDB18364_B0Gfd.seq ![]() |
>D07105D5_B0Gf_3_EGFP-C2_D07_04_ABI08.ab1 1 NNNNNNNNNN NNNNNANNNN NNNNCNNNTG GTCCTGCTGG ANTTCGTGAC CGCCGCCGGG 61 ATCACTCTCG GCATGNNNNA NCTGTACAAG GGTGCAGGCG CCCAAGCTTC GAATTCTGCA 121 GTCGACATGG TCTTCACACT CGAAGATTTC GTTGGGGACT GGCGACAGAC AGCCGGCTAC 181 AACCTGGACC AAGTCCTTGA ACAGGGAGGT GTGTCCAGTT TGTTTCAGAA TCTCGGGGTG 241 TCCGTAACTC CGATCCAAAG GATTGTCCTG AGCGGTGAAA ATGGGCTGAA GATCGACATC 301 CATGTCATCA TCCCGTATGA AGGTCTGAGC GGCGACCAAA TGGGCCAGAT CGAAAAAATT 361 TTTAAGGTGG TGTACCCTGT GGATGATCAT CACTTTAAGG TGATCCTGCA CTATGGCACA 421 CTGGTAATCG ACGGGGTTAC GCCGAACATG ATCGACTATT TCGGACGGCC GTATGAAGGC 481 ATCGCCGTGT TCGACGGCAA AAAGATCACT GTAACAGGGA CCCTGTGGAA CGGCAACAAA 541 ATTATCGACG AGCGCCTGAT CAACCCCGAC GGCTCCCTGC TGTTCCGAGT AACCATCAAC 601 GGAGTGACCG GCTGGCGGCT GTGCGAACGC ATTCTGGCGT AAGGATCCAC CGGATCTAGA 661 TAACTGATCA TAATCAGCCA TACCACATTT GNNNNNNNTT TANNNNNNTT AAAAAACCTC 721 CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTNNNNNN NTAACTTGTT 781 TATTGCAGCT TATAATGGTT ACNAAATAAA GCAATAGCAT CACNANTTTC ACNANTAAAG 841 CATTTTTTTC ACTGCNTTCT ANNTGTGGTT NGTNNNAANC TCATCAATGT ATCTTNNNGC 901 GTCGATCATA NTNCNATAAC CCTTANNATA NCTTCGNATA ATGNATGCTA TACNANNTTA 961 TTANNTCTGA AGANNANNTT NNGNCCANNC NANCTTNNNA TNNCGANCTC GAAATTCTNN 1021 NNGGTNNGGN NNGNGCTTTT CNNNNGNANN CNNNNNCANN CNNNTNNNNN NNNCNNNNNN 1081 NNNN // |
Primer: PGKpro_R (Pr0793) Region: PGKpro, loxP, SV40 pA, Nluc Sequence file: RDB18364_B0Gfe.seq ![]() |
>D07105D5_B0Gf_3_PGKpro_R_E07_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNGANN NNNNAGGCCN CTTGTGTAGC 61 GCCNAGTGCC CAGCGGGGCT GCTAANGCGC ATGCTCCAGA CTGCCTTGGG AAAAGCGCCT 121 CCCCTACCCG GTAGAATTTC GAGGTCGAGA TCCTAAGCTT GGCTGGACGT AAACTCCTCT 181 TCAGACCTAA TAACTTCGTA TAGCATACAT TATACGAAGT TATATTAAGG GTTATTGAAT 241 ATGATCGACG CGTTAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA 301 AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC 361 TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG 421 GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC TGATTATGAT 481 CAGTTATCTA GATCCGGTGG ATCCTTACGC CAGAATGCGT TCGCACAGCC GCCAGCCGGT 541 CACTCCGTTG ATGGTTACTC GGAACAGCAG GGAGCCGTCG GGGTTGATCA GGCGCTCGTC 601 GATAATTTTG TTGCCGTTCC ACAGGGTCCC TGTTACAGTG ATCTTTTTGC CGTCGAACAC 661 GGCGATGCCT TCNTACGGCC GTCCGAAATA GTCNATCATG TTCGGCGTAA CCCCGTCGAT 721 TACCAGTGTG CCATANNNNN NGATCACCTT AAAGTGATGA TCATCCNNNN NGTACACCAN 781 CCTTNAAANT TTTTTCNATC TGGCCNNTTT GNTCGCCGCT CAGACCTTCN TACGGGANGA 841 NGACNTGGAT GTCNATCTTN NNCCCATTTT CNNCGNTCAN GACAATCCTT TNNNTNNNNN 901 NNACNNANAC CCCGANANTT CTGAAACAAN TGNANACNNC TNCCTGNNNN NNNNNN // |
Primer: PGKpro_F (Pr0249) Region: HygR Sequence file: RDB18364_B0Gff.seq ![]() |
>D07105D5_B0Gf_3_PGKpro_F_F07_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTCT CCGGGCCTTT CGNNCTGCAT CCNTCTAGAT 61 CTCGAGCAGC TGAAGCTTAC CGCTAGCATG GATAGATCCG GAAAGCCTGA ACTCACCGCG 121 ACGTCTGTCG AGAAGTTTCT GATCGAAAAG TTCGACAGCG TCTCCGACCT GATGCAGCTC 181 TCGGAGGGCG AAGAATCTCG TGCTTTCAGC TTCGATGTAG GAGGGCGTGG ATATGTCCTG 241 CGGGTAAATA GCTGCGCCGA TGGTTTCTAC AAAGATCGTT ATGTTTATCG GCACTTTGCA 301 TCGGCCGCGC TCCCGATTCC GGAAGTGCTT GACATTGGGG AATTCAGCGA GAGCCTGACC 361 TATTGCATCT CCCGCCGTGC ACAGGGTGTC ACGTTGCAAG ACCTGCCTGA AACCGAACTG 421 CCCGCTGTTC TGCAGCCGGT CGCGGAGGCC ATGGATGCGA TCGCTGCGGC CGATCTTAGC 481 CAGACGAGCG GGTTCGGCCC ATTCGGACCG CAAGGAATCG GTCAATACAC TACATGGCGT 541 GATTTCATAT GCGCGATTGC TGATCCCCAT GTGTATCACT GGCAAACTGT GATGGACGAC 601 ACCGTCAGTG CGTCCGTCGC GCAGGCTCTC GATGAGCTGA TGCTTTGGGC CGAGGACTGC 661 CCCGAAGTCC GGCACCTCGT GCACGCGGAT TTCGGCTCCN ACAATGTCCT GACGGACAAT 721 GGCCGCATAA CAGCGGTCAT TGACTGGAGC GAGGCGATGT TCGGGGANTC CCAATACGAG 781 GTCGCCAACA TCTTCTTCTG GANGNCGTGG TTGGCTTGTA TGGAGCAGCA GANGCGCTAC 841 TTCGAGCGGA NGCATCCGGA GCTTGNANGA TCGCCGCGGC TNNNGGCGTA TATGCTCCGC 901 NTTGGTCTTG ANCNACTCTA TCANANNTTG GTTGANGGNN ATTTCGATGA NGCANNTTNN 961 NNNNNNTCNN NGNNANGCNN NCGTCCNATN NNNANNCNNG GNNNGNCNGG CGTANNNAAA 1021 TCNNNNCANA ANCNNGGNNN TCNNGNANNG NTNGNNNNNN NNNN // |
Primer: pUC-M13_-100 (Pr0310) Region: Tol2R175, loxP, mouse PGK ter Sequence file: RDB18364_B0Gfg.seq ![]() |
>D07105D5_B0Gf_3_pUC-M13_-100_G07_07_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCCNGGGT TTTCCCNGTC NCGACGTTGN 61 NNNNNGACGG CCAGTGAGCG CGCGNAATAC GACTCACTAT AGGGCGAATT GGGTACCGGC 121 ATATGGTTCT TGACAGAGGT GTAAAAAGTA CTCAAAAATT TTACTCAAGT GAAAGTACAA 181 GTACTTAGGG AAAATTTTAC TCAATTAAAA GTAAAAGTAT CTGGCTAGAA TCTTACTTGA 241 GTAAAAGTAA AAAAGTACTC CATTAAAATT GTACTTGAGT ATTAAGGAAG TAAAAGTAAA 301 AGCAAGAAAG ATCTTAGTGA ACCTCTTCGA GGGACCTAAT AACTTCGTAT AGCATACATT 361 ATACGAAGTT ATATTAAGGG TTCCGGATCT CGACCAGCTT CTGATGGAAT TAGAACTTGG 421 CAAAACAATA CTGAGAATGA AGTGTATGTG GAACAGAGGC TGCTGATCTC GTTCTTCAGG 481 CTATGAAACT GACACATTTG GAAACCACAG TACTTAGAAC CACAAAGTGG GAATCAAGAG 541 AAAAACAATG ATCCCACNAN AGATCTATAG ATCTATAGAT CATGAGTGGG AGGAATGAGC 601 TGGCCCTTAA TTTGGTTTTG CTTGTTTAAA TTATGATATC CAACTATGAA ACATTATCAT 661 AAAGCAATAG TAAAGAGCCT TCAGTAAAGA GCAGGCATTT ATCTAATCCC ACCCCACCCC 721 CACCCCCGTA GCTCCNATCC TTCCATTCAA AATGTANGTA CTCTGTTCTC ACCCTTCTNN 781 ACAAAGTANG ACAGGAAAAA CTTCCATTTT ANTGNACATC TTTATTGNTT AATAGATCAT 841 CAATTTCTGC ATCCCGGGGA NCTGANATCN TCGATGCNTG GGGGTCGTGC GCTCCTTTCG 901 GTCGGGCGCT GCGGGGTCNN GGGGCGGGCG GNTANNNNAT TCCNTNGCCN TCNNANNANN 961 GCTGGGGCGT NNGTTTNCAC TATCNNNNAG TNNNNNNNCN NAGCCNTCGG NCCNNANNNN 1021 NNCNCTTCNN NNGNNNNANT NNNGNANNNC NNNNNNNNCC NGNNNCCNGN ANNNNNNNNN 1081 ANNNNNNNNN N // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP] |
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