Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK411

TOL2 expression vector of OsTIR1(F74G) and mAID-EGFP-Nluc marker.

Catalog number RDB18364
Resource name pMK411
Alternative name OsTIR1(F74G)mAID-EGFP-Nluc
Clone info. TOL2 expression vector of OsTIR1(F74G) and mAID-EGFP-Nluc marker, see Fig. 6 of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone pT2AL200R175-CAGGS-EGFP (plasmid)
Selectable markers Ampicillin
Gene/insert name Oryza sativa OsTIR1 cDNA Arabidopsis thaliana IAA17 cDNA Aequorea victoria GFP (EGFP) cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank

External Database
Oryza sativa OsTIR1 Arabidopsis thaliana IAA17 Aequorea victoria GFP (EGFP)

        Reference sequence
          
         
        Sequence (full) RDB18364zzk01.seq provided by the depositor

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
        Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
        The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Koichi Kawakami of the National Institute of Genetics (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004))is requested.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
        提供案内 (日本国内) [open/close]

        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
        付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
        Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。利用者は、研究成果の公表にあたって国立遺伝学研究所 川上浩一博士が指定する文献 (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004)) の引用を必要とする。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB18364 pMK411 DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Original reference

        original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Auxin Inducible Degron (AID) System (English text)
        Featured content Auxin Inducible Degron (AID) System (Japanese text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB18364_B0Gfp1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: reverse-2
        Region: Tol2L200,CMV en,chicken beta-actin pro,
        Sequence file: RDB18364_B0Gfa.seq check
        >D07105D5_B0Gf_3_reverse-2_A07_01_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNCGCNNN NNNNNCTCAC TAAAGGGAAC AAAAGCTGGA
           61 GCTCGGATCT GCTGGGCTNG NNNAAGGTAG GGGGTCAAGA ACCAGAGGTG TAAAGTACTT
          121 GAGTAATTTT ACTTGATTAC TGTACTTAAG TATTATTTTT GGGGATTTTT ACTTTACTTG
          181 AGTACAATTA AAAATCAATA CTTTTACTTT TACTTAATTA CATTTTTTTA GAAAAAAAAG
          241 TACTTTTTAC TCCTTACAAT TTTATTTACA GTCAAAAAGT ACTTATTTTT TGGAGATCAC
          301 TTGGGCCCTC TAGATGCATG CTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT
          361 TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA
          421 TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT
          481 TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA
          541 AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT
          601 CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC
          661 TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTCGAGG TGAGCCCCAC
          721 GTTCTGCTTC ACTCTCCCCA TCTCCCCCCC CTCCCCACCC CCAATTTTGT ATTTATTTAT
        781 TTTTNNATTA TTTTGTGCAG CGATGGGGGC GNNGGGGGGG GNNNNNNNNN N
        //
        Primer: pCAG-F_V3
        Region: chimeric intron, OsTIR1(F74G) 5'
        Sequence file: RDB18364_B0Gfb.seq check
        >D07105D5_B0Gf_3_pCAG-F_V3_B07_02_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NTNNNNNNNN NNNNNNGCAN NGCGGNNNNN NNCTTCTGGC
           61 GTGTGACCGG CGGCTCTANA NNNTCTGCNN NNCNTGTTCA TGCCTTCTTC TTTTTCCTAC
          121 AGCTCCTGGG CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCGAGC
          181 TCGGTACCTC GCGAATGCAT CTAGATAGCG CTGCCACCAT GACATACTTT CCTGAAGAGG
          241 TCGTCGAACA CATTTTTAGC TTCCTGCCTG CACAGAGAGA TAGAAACACA GTGAGCCTGG
          301 TCTGCAAAGT GTGGTACGAG ATCGAACGCC TGAGCCGGAG AGGAGTGTTC GTCGGCAACT
          361 GCTATGCTGT GAGAGCAGGC AGGGTCGCCG CTAGGTTTCC AAATGTGCGC GCACTGACCG
          421 TCAAGGGGAA ACCCCACGGC GCCGACTTTA ACCTGGTGCC CCCTGATTGG GGAGGATACG
          481 CCGGCCCTTG GATCGAGGCA GCCGCTCGCG GCTGTCATGG ACTGGAGGAA CTGCGCATGA
          541 AGCGAATGGT GGTCTCTGAC GAAAGTCTGG AGCTGCTGGC TCGGAGCTTC CCTAGGTTTC
          601 GCGCACTGGT GCTGATTTCT TGCGAAGGCT TCAGCACCGA TGGACTGGNN NNCGTGGCCT
          661 CCCACTGTAA GCTGCTGCGG GAGCTGGACC TCCAGGAGAA TGAANTGGAG GATAGAGGCC
          721 CCAGATGGCT GTCTTGCTTN CCAGACTCAT GTACCAGCCT GGTGTCCCTG AACTTTGCCT
          781 GCNTCNANNN CGANNTGAAT GCTGGGTCCC TGNANCGGNT GGTCTNNNNA AGCCCCAACC
          841 TGAGGNCTCT GCNGNNTNAN CCGGANCGNN NNNGTGNNNA CTNTGGCNNA NNANNCTNNN
          901 NGAGAANCCC TANCCTGNNN GANCTNGGAA NNCGNNNANN NTGANANANG ANTTTCNNNA
          961 NNNNAATNCN NNNTTTTANN NTGNNNNTCT GCCCNNGNNN NNANNNNNNN NAANGNNNAN
         1021 GNNNNNNGNC NNNGATTCNN NGGNNNGNTN NNNNNNNNGN NNNCNNGANN NTTNANNNTN
        1081 NCCCNNNNNN NNNN
        //
        Primer: YFP_R
        Region: EGFP 5', mAID, P2A, OsTIR1(F74G) 3'
        Sequence file: RDB18364_B0Gfc.seq check
        >D07105D5_B0Gf_3_YFP_R_C07_03_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC NNNNNNTGTG GCCGTTTACG TCGCCGTCCA
           61 GCTCGACCAG GATGGNCNNN NNNNNGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGCTAG
          121 CTTTATACAT CCTCAAATCG ATTTTCCTCA AGTACGGTGC TCCGTCCATT GATACCTTCA
          181 CGAACGCCGC CGCCTCCGGG CCACCGCTTG ATTTTTGGCA GGAAACCATC ACGTTCTTCC
          241 GGTATGATCT CACCGGTGGC CATCCCACAA CTTGTGCCTT GGCCGGAGGT TTGGCTGGAT
          301 CTTTAGGACA AGCACTCTTC TCCTTAGATC TAGGTCCAGG GTTCTCCTCC ACGTCTCCAG
          361 CCTGCTTCAG CAGGCTGAAG TTAGTAGCTC CGCTTCCTGA TCTCAGAATC TTCACAAAGT
          421 TGGGAGCATC ATCTCTTGCC CCTGCGGTGG TCCGATACAC ATACAGTTTC TCCACCTTTG
          481 GCAGGTCGCC ATGATTTTCC TCCATTTCGT TAGACCCGTC CCGCTCATTA ATGACTTCCA
          541 CGTTCAGCAT AGGCATTTTA CTAGCCAGGA CCTGACAGCC CTTCAGAGTC ACATTGCAAC
          601 TAGACATCCA CAGGCTCCTC ATTGTCTCGT ATCTGGCAAA GTTTCCCAGC AGAGCGGCAT
          661 CCCCGAAAGG GCTGTCCCGG ATTTCCAGTT TTCGCAGATT CTTACACCCG TTCATCACGT
          721 GCATCATGCC CTTATCGGAN TCTCCGGCAA ATGCAATGCT CAGCATCTCC AGCTGCTTAN
          781 NNTACNTTGC CGATGTACAT GAACACTTTG TCTGTCANCA GGNCNGAAAT GNTCANCCTC
          841 TGGANTCCCT TGCACTCTCT CACGATAGCT CCAAACCCTT CATCCAGTGG NTGGNNTGNG
          901 ACCACGTCGG GTTTGNCTGG NTCNNNAANA NACANCNNAA ANCNGGTGAA ATTANGGGNA
        961 GTTCNNGNNN ANNGNNNCCA NTGNANNANN NNNNNATCTG ATGANANAAN NNNNNNNNN
        //
        Primer: EGFP-C2
        Region: EGFP 3', Linker, Nluc,
        Sequence file: RDB18364_B0Gfd.seq check
        >D07105D5_B0Gf_3_EGFP-C2_D07_04_ABI08.ab1
            1 NNNNNNNNNN NNNNNANNNN NNNNCNNNTG GTCCTGCTGG ANTTCGTGAC CGCCGCCGGG
           61 ATCACTCTCG GCATGNNNNA NCTGTACAAG GGTGCAGGCG CCCAAGCTTC GAATTCTGCA
          121 GTCGACATGG TCTTCACACT CGAAGATTTC GTTGGGGACT GGCGACAGAC AGCCGGCTAC
          181 AACCTGGACC AAGTCCTTGA ACAGGGAGGT GTGTCCAGTT TGTTTCAGAA TCTCGGGGTG
          241 TCCGTAACTC CGATCCAAAG GATTGTCCTG AGCGGTGAAA ATGGGCTGAA GATCGACATC
          301 CATGTCATCA TCCCGTATGA AGGTCTGAGC GGCGACCAAA TGGGCCAGAT CGAAAAAATT
          361 TTTAAGGTGG TGTACCCTGT GGATGATCAT CACTTTAAGG TGATCCTGCA CTATGGCACA
          421 CTGGTAATCG ACGGGGTTAC GCCGAACATG ATCGACTATT TCGGACGGCC GTATGAAGGC
          481 ATCGCCGTGT TCGACGGCAA AAAGATCACT GTAACAGGGA CCCTGTGGAA CGGCAACAAA
          541 ATTATCGACG AGCGCCTGAT CAACCCCGAC GGCTCCCTGC TGTTCCGAGT AACCATCAAC
          601 GGAGTGACCG GCTGGCGGCT GTGCGAACGC ATTCTGGCGT AAGGATCCAC CGGATCTAGA
          661 TAACTGATCA TAATCAGCCA TACCACATTT GNNNNNNNTT TANNNNNNTT AAAAAACCTC
          721 CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTNNNNNN NTAACTTGTT
          781 TATTGCAGCT TATAATGGTT ACNAAATAAA GCAATAGCAT CACNANTTTC ACNANTAAAG
          841 CATTTTTTTC ACTGCNTTCT ANNTGTGGTT NGTNNNAANC TCATCAATGT ATCTTNNNGC
          901 GTCGATCATA NTNCNATAAC CCTTANNATA NCTTCGNATA ATGNATGCTA TACNANNTTA
          961 TTANNTCTGA AGANNANNTT NNGNCCANNC NANCTTNNNA TNNCGANCTC GAAATTCTNN
         1021 NNGGTNNGGN NNGNGCTTTT CNNNNGNANN CNNNNNCANN CNNNTNNNNN NNNCNNNNNN
        1081 NNNN
        //
        Primer: PGKpro_R
        Region: PGKpro, loxP, SV40 pA, Nluc
        Sequence file: RDB18364_B0Gfe.seq check
        >D07105D5_B0Gf_3_PGKpro_R_E07_05_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNGANN NNNNAGGCCN CTTGTGTAGC
           61 GCCNAGTGCC CAGCGGGGCT GCTAANGCGC ATGCTCCAGA CTGCCTTGGG AAAAGCGCCT
          121 CCCCTACCCG GTAGAATTTC GAGGTCGAGA TCCTAAGCTT GGCTGGACGT AAACTCCTCT
          181 TCAGACCTAA TAACTTCGTA TAGCATACAT TATACGAAGT TATATTAAGG GTTATTGAAT
          241 ATGATCGACG CGTTAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA
          301 AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC
          361 TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG
          421 GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC TGATTATGAT
          481 CAGTTATCTA GATCCGGTGG ATCCTTACGC CAGAATGCGT TCGCACAGCC GCCAGCCGGT
          541 CACTCCGTTG ATGGTTACTC GGAACAGCAG GGAGCCGTCG GGGTTGATCA GGCGCTCGTC
          601 GATAATTTTG TTGCCGTTCC ACAGGGTCCC TGTTACAGTG ATCTTTTTGC CGTCGAACAC
          661 GGCGATGCCT TCNTACGGCC GTCCGAAATA GTCNATCATG TTCGGCGTAA CCCCGTCGAT
          721 TACCAGTGTG CCATANNNNN NGATCACCTT AAAGTGATGA TCATCCNNNN NGTACACCAN
          781 CCTTNAAANT TTTTTCNATC TGGCCNNTTT GNTCGCCGCT CAGACCTTCN TACGGGANGA
          841 NGACNTGGAT GTCNATCTTN NNCCCATTTT CNNCGNTCAN GACAATCCTT TNNNTNNNNN
        901 NNACNNANAC CCCGANANTT CTGAAACAAN TGNANACNNC TNCCTGNNNN NNNNNN
        //
        Primer: PGKpro_F
        Region: HygR
        Sequence file: RDB18364_B0Gff.seq check
        >D07105D5_B0Gf_3_PGKpro_F_F07_06_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTCT CCGGGCCTTT CGNNCTGCAT CCNTCTAGAT
           61 CTCGAGCAGC TGAAGCTTAC CGCTAGCATG GATAGATCCG GAAAGCCTGA ACTCACCGCG
          121 ACGTCTGTCG AGAAGTTTCT GATCGAAAAG TTCGACAGCG TCTCCGACCT GATGCAGCTC
          181 TCGGAGGGCG AAGAATCTCG TGCTTTCAGC TTCGATGTAG GAGGGCGTGG ATATGTCCTG
          241 CGGGTAAATA GCTGCGCCGA TGGTTTCTAC AAAGATCGTT ATGTTTATCG GCACTTTGCA
          301 TCGGCCGCGC TCCCGATTCC GGAAGTGCTT GACATTGGGG AATTCAGCGA GAGCCTGACC
          361 TATTGCATCT CCCGCCGTGC ACAGGGTGTC ACGTTGCAAG ACCTGCCTGA AACCGAACTG
          421 CCCGCTGTTC TGCAGCCGGT CGCGGAGGCC ATGGATGCGA TCGCTGCGGC CGATCTTAGC
          481 CAGACGAGCG GGTTCGGCCC ATTCGGACCG CAAGGAATCG GTCAATACAC TACATGGCGT
          541 GATTTCATAT GCGCGATTGC TGATCCCCAT GTGTATCACT GGCAAACTGT GATGGACGAC
          601 ACCGTCAGTG CGTCCGTCGC GCAGGCTCTC GATGAGCTGA TGCTTTGGGC CGAGGACTGC
          661 CCCGAAGTCC GGCACCTCGT GCACGCGGAT TTCGGCTCCN ACAATGTCCT GACGGACAAT
          721 GGCCGCATAA CAGCGGTCAT TGACTGGAGC GAGGCGATGT TCGGGGANTC CCAATACGAG
          781 GTCGCCAACA TCTTCTTCTG GANGNCGTGG TTGGCTTGTA TGGAGCAGCA GANGCGCTAC
          841 TTCGAGCGGA NGCATCCGGA GCTTGNANGA TCGCCGCGGC TNNNGGCGTA TATGCTCCGC
          901 NTTGGTCTTG ANCNACTCTA TCANANNTTG GTTGANGGNN ATTTCGATGA NGCANNTTNN
          961 NNNNNNTCNN NGNNANGCNN NCGTCCNATN NNNANNCNNG GNNNGNCNGG CGTANNNAAA
        1021 TCNNNNCANA ANCNNGGNNN TCNNGNANNG NTNGNNNNNN NNNN
        //
        Primer: pUC-M13_-100
        Region: Tol2R175, loxP, mouse PGK ter
        Sequence file: RDB18364_B0Gfg.seq check
        >D07105D5_B0Gf_3_pUC-M13_-100_G07_07_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCCNGGGT TTTCCCNGTC NCGACGTTGN
           61 NNNNNGACGG CCAGTGAGCG CGCGNAATAC GACTCACTAT AGGGCGAATT GGGTACCGGC
          121 ATATGGTTCT TGACAGAGGT GTAAAAAGTA CTCAAAAATT TTACTCAAGT GAAAGTACAA
          181 GTACTTAGGG AAAATTTTAC TCAATTAAAA GTAAAAGTAT CTGGCTAGAA TCTTACTTGA
          241 GTAAAAGTAA AAAAGTACTC CATTAAAATT GTACTTGAGT ATTAAGGAAG TAAAAGTAAA
          301 AGCAAGAAAG ATCTTAGTGA ACCTCTTCGA GGGACCTAAT AACTTCGTAT AGCATACATT
          361 ATACGAAGTT ATATTAAGGG TTCCGGATCT CGACCAGCTT CTGATGGAAT TAGAACTTGG
          421 CAAAACAATA CTGAGAATGA AGTGTATGTG GAACAGAGGC TGCTGATCTC GTTCTTCAGG
          481 CTATGAAACT GACACATTTG GAAACCACAG TACTTAGAAC CACAAAGTGG GAATCAAGAG
          541 AAAAACAATG ATCCCACNAN AGATCTATAG ATCTATAGAT CATGAGTGGG AGGAATGAGC
          601 TGGCCCTTAA TTTGGTTTTG CTTGTTTAAA TTATGATATC CAACTATGAA ACATTATCAT
          661 AAAGCAATAG TAAAGAGCCT TCAGTAAAGA GCAGGCATTT ATCTAATCCC ACCCCACCCC
          721 CACCCCCGTA GCTCCNATCC TTCCATTCAA AATGTANGTA CTCTGTTCTC ACCCTTCTNN
          781 ACAAAGTANG ACAGGAAAAA CTTCCATTTT ANTGNACATC TTTATTGNTT AATAGATCAT
          841 CAATTTCTGC ATCCCGGGGA NCTGANATCN TCGATGCNTG GGGGTCGTGC GCTCCTTTCG
          901 GTCGGGCGCT GCGGGGTCNN GGGGCGGGCG GNTANNNNAT TCCNTNGCCN TCNNANNANN
          961 GCTGGGGCGT NNGTTTNCAC TATCNNNNAG TNNNNNNNCN NAGCCNTCGG NCCNNANNNN
         1021 NNCNCTTCNN NNGNNNNANT NNNGNANNNC NNNNNNNNCC NGNNNCCNGN ANNNNNNNNN
        1081 ANNNNNNNNN N
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522.

        2022.08.12

        GNP_filter3_RDBDEP_html_220812.pl