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pMK279 (mClover-Bsr)

A plasmid for construction of C-terminal mClover tagging donors.

Catalog number RDB13922
Resource name pMK279 (mClover-Bsr)
Alternative name mClover-Bsr, 999
Clone info. A plasmid for construction of C-terminal mClover tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone.
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Schedule A (GFP-A) [Link]
            Remarks
            Please use Schedule A (GFP-A) additionally.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            Schedule A(GFP-A, 英語版のみ) [Link]
            備考
            Schedule A(GFP-A, 英語版のみ)が追加で必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB13922 pMK279 (mClover-Bsr) DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Auxin Inducible Degron (AID) System (English text)
            Featured content Auxin Inducible Degron (AID) System (Japanese text)
            Featured content Genome Editing (English text)
            Featured content Genome Editing (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13922_A6B8p1_v2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: M13_-40
            Sequence file: RDB13922_A6B8a_2.seq check
            >D03070A06_A6B8_1_M13(-40)_C02_08.ab1
                1 CGTCGGACGC CGCGTATACG ACTCACTATA GGGCGAATTG GAGCTCGGAT CCGGTGCAGG
               61 CGCCGCTAGC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT
              121 CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTC CGCGGCGAGG GCGAGGGCGA
              181 TGCCACCAAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC
              241 CTGGCCCACC CTCGTGACCA CCTTCGGCTA CGGCGTGGCC TGCTTCAGCC GCTACCCCGA
              301 CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG
              361 CACCATCTCT TTCAAGGACG ACGGTACCTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG
              421 CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT
              481 CCTGGGGCAC AAGCTGGAGT ACAACTTCAA CAGCCACAAC GTCTATATCA CGGCCGACAA
              541 GCAGAAGAAC GGCATCAAGG CTAACTTCAA GATCCGCCAC AACGTTGAGG ACGGCAGCGT
              601 GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC
              661 CGACAACCAC TACCTGAGCC ATCAGTCCAA GCTGAGCAAA GACCCCAACG AGAAGCGCGA
              721 TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATT ACACATGGCA TGGACGAGCT
              781 GTACAAGTAA TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT
              841 TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT
              901 TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA
              961 TTTCACAAAT AAAGCATTTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA
             1021 ATGTATCTTA ACGCGTCGAT CATATTCATT ACCCTAATAT AACTTCCGTA TATGTATGGC
             1081 TATACGGAGG TATAGTCTGA GAGAGTTAAC GTCCAGTCAA GCCTTAGTAT CCCTCGGAAC
            1141 CCCTTCGA
            //
            Primer: PGKpro_F
            Sequence file: RDB13922_A6B8b_2.seq check
            >D03070A06_A6B8_1_PGK-proF_B07_04.ab1
                1 ATTGGGCGTT CTCTCTTCTC ATCTCCGGGC CTTTCGACCT GCATCCATCT AGATCTCGAG
               61 CAGCTGAAGC TTACCGCTAG CATGAAAACA TTTAACATTT CTCAACAAGA TCTAGAATTA
              121 GTAGAAGTAG CGACAGAGAA GATTACAATG CTTTATGAGG ATAATAAACA TCATGTGGGA
              181 GCGGCAATTC GTACGAAAAC AGGAGAAATC ATTTCGGCAG TACATATTGA AGCGTATATA
              241 GGACGAGTAA CTGTTTGTGC AGAAGCCATT GCGATTGGTA GTGCAGTTTC GAATGGACAA
              301 AAGGATTTTG ACACGATTGT AGCTGTTAGA CACCCTTATT CTGACGAAGT AGATAGAAGT
              361 ATTCGAGTGG TAAGTCCTTG TGGTATGTGT AGGGAGTTGA TTTCAGACTA TGCACCAGAT
              421 TGTTTTGTGT TAATAGAAAT GAATGGCAAG TTAGTCAAAA CTACGATTGA AGAACTCATT
              481 CCACTCAAAT ATACCCGAAA TTAAGCTAGC CGCCCGCCCC ACGACCCGCA GCGCCCGACC
              541 GAAAGGAGCG CACGACCCCA TGCATCGATG ATATCAGATC CCCGGGATGC AGAAATTGAT
              601 GATCTATTAA ACAATAAAGA TGTCCACTAA AATGGAAGTT TTTCCTGTCA TACTTTGTTA
              661 AGAAGGGTGA GAACAGAGTA CCTACATTTT GAATGGAAGG ATTGGAGCTA CGGGGGTGGG
            721 GGTGGGGTGG G
            //
            Primer: PGK_N_rev
            Sequence file: RDB13922_A6B8c_2.seq check
            >D03070A06_A6B8_1_PGK-N-rev_B06_06.ab1
                1 CGTGACGCTC CCTACCCGGT AGATTTCGAG GTCGAGATCC TAAGCTTGGC TGGACGTAAA
               61 CTCCTCTTCA GACCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ATTAAGGGTT
              121 ATTGAATATG ATCGACGCGT TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT
              181 GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT
              241 TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA
              301 GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA
              361 TTATGATCAG TTATCTAGAT TACTTGTACA GCTCGTCCAT GCCATGTGTA ATCCCGGCGG
              421 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGCT
              481 TGGACTGATG GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG
              541 TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CTCAACGTTG TGGCGGATCT
              601 TGAAGTTAGC CTTGATGCCG TTCTTCTGCT TGTCGGCCGT GATATAGACG TTGTGGCTGT
              661 TGAAGTTGTA CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT
              721 TCAGCTCGAT GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT
              781 AGGTACCGTC GTCCTTGAAA GAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG
              841 ACTTGAAGAA GTCGTGCTGC TTCATGTGGT CGGGGTAGCG GCTGAAGCAG CCACGCCGTA
              901 GCCGAAGGTG GTCACGAGGG TGGGCCAGGG CACGGGCAGC TTGCCCGGTG GTGCAGATGA
              961 ACTTCAGGGT CAGCTTTGCC GTTGGTGGCA TCGCCCTCGC CCTCGCGCGG ACGCTGAACT
             1021 TGTGGCCGTT TACGTCGCCG TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT
             1081 CTCGCCCTGC TCAACATGCT AGCGCGCTGC ACGATCGAGC TCATTCGCCT ATTGTTGGAA
            1141 GTCGGTAAAT TAAACGACG
            //
            Primer: M13R
            Sequence file: RDB13922_A6B8d_2.seq check
            >D03070A06_A6B8_1_M13R_C01_07.ab1
                1 TGTCGCGGGC ATTAACCCTC ACTAAAGGGA ACAAAAGCTG GGTACCGGAT CCTAGTGAAC
               61 CTCTTCGAGG GACCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ATTAAGGGTT
              121 CCGGATCTCG ACCAGCTTCT GATGGAATTA GAACTTGGCA AAACAATACT GAGAATGAAG
              181 TGTATGTGGA ACAGAGGCTG CTGATCTCGT TCTTCAGGCT ATGAAACTGA CACATTTGGA
              241 AACCACAGTA CTTAGAACCA CAAAGTGGGA ATCAAGAGAA AAACAATGAT CCCACGAGAG
              301 ATCTATAGAT CTATAGATCA TGAGTGGGAG GAATGAGCTG GCCCTTAATT TGGTTTTGCT
              361 TGTTTAAATT ATGATATCCA ACTATGAAAC ATTATCATAA AGCAATAGTA AAGAGCCTTC
              421 AGTAAAGAGC AGGCATTTAT CTAATCCCAC CCCACCCCCA CCCCCGTAGC TCCAATCCTT
              481 CCATTCAAAA TGTAGGTACT CTGTTCTCAC CCTTCTTAAC AAAGTATGAC AGGAAAAACT
              541 TCCATTTTAG TGGACATCTT TATTGTTTAA TAGATCATCA ATTTCTGCAT CCCGGGGATC
              601 TGATATCATC GATGCATGGG GTCGTGCGCT CCTTTCGGTC GGGCGCTGCG GGTCGTGGGG
              661 CGGGCGGCTA GCTTAATTTC GGGTATATTT GAGTGGAATG AAGTTCTTCA ATCGTAGTTT
              721 TGACTAAATT GCCCATTCAT TTCTATTAAC ACAAAACAAT CTGGTGCATA GTCTGAAAAT
            781 CAACTCCCTA CACATACCAC AAGGACTTTA CCACTCGAAA TACTTCTATC TACTT
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl