A plasmid for construction of C-terminal mClover tagging donors.
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External Database Aequorea victoria GFP Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
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Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ![]() |
提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。 |
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提供依頼 | 手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word ![]() 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word ![]() |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB13922 | pMK279 (mClover-Bsr) | DNA solution |
Materials & Methods section:
The pMK279 (mClover-Bsr) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13922). |
Reference section:
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Auxin Inducible Degron (AID) System (English text) |
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Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
Featured content | Genome Editing (English text) |
Featured content | Genome Editing (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13922_A6B8p1-3.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: M13_-40 (Pr0410) Region: T7 pro,insert 5'(Linker,mClover 5') Sequence file: RDB13922_A6B8e.seq ![]() |
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>D03070A06_A6B8_1_M13(-40)_C02_08.ab1 1 NNNNNNNNNN NNNNNNNNNG NNTNNCNATA GGGCGAATTG GAGCTCGGAT CCGGTGCAGG 61 CGCCGCTAGC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT 121 CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTC CGCGGCGAGG GCGAGGGCGA 181 TGCCACCAAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC 241 CTGGCCCACC CTCGTGACCA CCTTCGGCTA CGGCGTGGCC TGCTTCAGCC GCTACCCCGA 301 CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG 361 CACCATCTCT TTCAAGGACG ACGGTACCTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG 421 CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT 481 CCTGGGGCAC AAGCTGGAGT ACAACTTCAA CAGCCACAAC GTCTATATCA CGGCCGACAA 541 GCAGAAGAAC GGCATCAAGG CTAACTTCAA GATCCGCCAC AACGTTGAGG ACGGCAGCGT 601 GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC 661 CGACAACCAC TACCTGAGCC ATCAGTCCAA GCTGANCAAA GACCCCAACG AGAAGCGCGA 721 TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGNTT ACACATGGCA TGGACGANCT 781 GTACANGTAA TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG ANGTTTTACT 841 NGCTTTNAAA AACCNCCCAC ACCNNCCCCT GAANCNGAAA NNTAAAATGA ATGNNANTGT 901 TGNTGNTNAC TNGNTTANNG NANCTNANNA NGGNTACNAA NAAAGCNANA NCATCNCNAN 961 TTTCNNNAAT NAANNNNTTT TTTNNNNNNN NNN // |
Primer: M13R (Pr0160) Region: insert 3'(loxP,mPGK ter) Sequence file: RDB13922_A6B8f.seq ![]() |
>D03070A06_A6B8_1_M13R_C01_07.ab1 1 NNNNNNNNNN NNNNNNNNNN NCNAANGGNN NNNNNNNCTG GGTANCGGAT CCTAGTGAAC 61 CTCTTCGAGG GANCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ATTAAGGGTT 121 CCGGATCTCG ACCAGCTTCT GATGGAATTA GAACTTGGCA AAACAATACT GAGAATGAAG 181 TGTATGTGGA ACAGAGGCTG CTGATCTCGT TCTTCAGGCT ATGAAACTGA CACATTTGGA 241 AACCACAGTA CTTAGAACCA CAAAGTGGGA ATCAAGAGAA AAACAATGAT CCCACGAGAG 301 ATCTATAGAT CTATAGATCA TGAGTGGGAG GAATGAGCTG GCCCTTAATT TGGTTTTGCT 361 TGTTTAAATT ATGATATNNN NNTATGAAAC ATTATCATAA AGCAATANNA NAGAGCCTTC 421 AGTAAAGANC NNGCATTNNN NNAATCCCAC CCCACCCCCA CCCCCGTAGC TCCAATCCTT 481 CCATTCAAAA TGTAGGTNNN NNNTTCTCAC CCTTCNTANC AAAGTANGAN NNGAAAAACT 541 TNNNNTTTAG TGNACNTCTT TATTGTTTAA TNNNNNNNNN N // |
Primer: PGK_N_rev (Pr0243) Region: insert mid(loxP,SV40 pA,mClover 3') Sequence file: RDB13922_A6B8g.seq ![]() |
>D03070A06_A6B8_1_PGK-N-rev_B06_06.ab1 1 NNNNNNNNNN CNNNNNNGGN NNANTTCGAG GTCGAGATCC TAAGCTTGGC TGGACGTAAA 61 CTCCTCTTCA GACCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ATTAAGGGTT 121 ATTGAATATG ATCGACGCGT TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT 181 GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT 241 TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA 301 GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA 361 TTATGATCAG TTATCTAGAT TACTTGTACA GCTCGTCCAT GCCATGTGTA ATCCCGGCGG 421 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGCT 481 TGGACTGATG GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG 541 TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CTCAACGTTG TGGCGGATCT 601 TGAAGTTAGC CTTGATGCCG TTCTTCTGCT TGTCGGCCGT GATATAGACG TTGTGGCTGT 661 TGAAGTTGTA CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT 721 TCAGCTCGAT GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT 781 ANGTACCGTC GTCCTTGAAA GAGATGGTGC GCTCCTGGAC GTANCCTTCG GGCATGGCGG 841 ANTTGAANAA NTCGTGCTGC TTCATGTGGT CGGGGTANCG GCTGAAGCNN NCACGCCGTA 901 NCCNAANGNG GTCNCGANGG TGGGNCAGGG NACNGGNANC TTGNCNNNTG GNGNNNANGA 961 NNNTNNNGGT CANCNTNGNC NNNNGNNGNA TNGCCNNNNN CNNCNNNNGN NNNNNNNNN // |
Primer: PGKpro_F (Pr0249) Region: insert mid(Bsr,mPGK ter) Sequence file: RDB13922_A6B8h.seq ![]() |
>D03070A06_A6B8_1_PGK-proF_B07_04.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNTCCNGGC CTTTCGACCT GCATCCNTCT AGATCTCGAG 61 CAGCTGAAGC TTACCGCTAG CATGAAAACA TTTAACATTT CTCAACAAGA TCTAGAATTA 121 GTAGAAGTAG CGACAGAGAA GATTACAATG CTTTATGAGG ATAATAAACA TCATGTGGGA 181 GCGGCAATTC GTACGAAAAC AGGAGAAATC ATTTCGGCAG TACATATTGA AGCGTATATA 241 GGACGAGTAA CTGTTTGTGC AGAAGCCATT GCGATTGGTA GTGCAGTTTC GAATGGACAA 301 AAGGATTTTG ACACGATTGT AGCTGTTAGA CACCCTTATT CTGACGAAGT AGATAGAAGT 361 ATTCGAGTGG TAAGTCCTTG TGGTATGTGT AGGGAGTTGA TTTCAGACTA TGCACCAGAT 421 TGTTTTGTGT TAATAGAAAT GAATGGCAAG TTAGTCAAAA CTACGATTGA AGAACTCATT 481 CCACTCAAAT ATACCCGAAA TTAAGCTAGC CGCCCGCCCC ACGACCCGCA GCGCCCGACC 541 GAAAGGAGCG CACGACCCCA TGCATCGATG ATATCAGATC CCCGGGATGC AGAAATTGAT 601 GATCTATTAA ACAATAAAGA TGTCCACTAA AATGGAAGTT TTTCCTGTCA TACTTTGTTA 661 AGAAGGGTGA GAACAGAGTA CCTACATTTT GAATGGAAGG ANTGGAGCTA CGGGGGTGGG 721 GGTGGGGTGG GATTAGATAA ATGCCTGCTC TTTACTGAAN GNTCTTTACT ATTGCTTTAT 781 GATAATGTTT CATAGTTGGA TATCATAATT TAAACNAGCN AAACCAANTT NANNGNCNNG 841 CTNATTCCNC CCACTCANGA TCTATAGATC TATAGATCTC TCNNGGGATC ATTGNTTTTN 901 NTCTNGATTN CCANTTTGTG GNTCTAAGTA CTGNNGNNTT NNNAANGTGT CNNTTTNNNA 961 GCCNGANNNA CGNNNTNAGC NNNCNNNGNT NCNNNNNNNN NN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. |
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2023.05.01
GNP_filter3_RDBDEP_html_230501.pl