Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK279 (mClover-Bsr)

A plasmid for construction of C-terminal mClover tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

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Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13922_A6B8p1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410) Region: T7 pro,insert 5'(Linker,mClover 5') Sequence file: RDB13922_A6B8e.seq
>D03070A06_A6B8_1_M13(-40)_C02_08.ab1     1 NNNNNNNNNN NNNNNNNNNG NNTNNCNATA GGGCGAATTG GAGCTCGGAT CCGGTGCAGG    61 CGCCGCTAGC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT   121 CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTC CGCGGCGAGG GCGAGGGCGA   181 TGCCACCAAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC   241 CTGGCCCACC CTCGTGACCA CCTTCGGCTA CGGCGTGGCC TGCTTCAGCC GCTACCCCGA   301 CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG   361 CACCATCTCT TTCAAGGACG ACGGTACCTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG   421 CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT   481 CCTGGGGCAC AAGCTGGAGT ACAACTTCAA CAGCCACAAC GTCTATATCA CGGCCGACAA   541 GCAGAAGAAC GGCATCAAGG CTAACTTCAA GATCCGCCAC AACGTTGAGG ACGGCAGCGT   601 GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC   661 CGACAACCAC TACCTGAGCC ATCAGTCCAA GCTGANCAAA GACCCCAACG AGAAGCGCGA   721 TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGNTT ACACATGGCA TGGACGANCT   781 GTACANGTAA TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG ANGTTTTACT   841 NGCTTTNAAA AACCNCCCAC ACCNNCCCCT GAANCNGAAA NNTAAAATGA ATGNNANTGT   901 TGNTGNTNAC TNGNTTANNG NANCTNANNA NGGNTACNAA NAAAGCNANA NCATCNCNAN 961 TTTCNNNAAT NAANNNNTTT TTTNNNNNNN NNN //
Primer: M13R (Pr0160) Region: insert 3'(loxP,mPGK ter) Sequence file: RDB13922_A6B8f.seq
>D03070A06_A6B8_1_M13R_C01_07.ab1     1 NNNNNNNNNN NNNNNNNNNN NCNAANGGNN NNNNNNNCTG GGTANCGGAT CCTAGTGAAC    61 CTCTTCGAGG GANCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ATTAAGGGTT   121 CCGGATCTCG ACCAGCTTCT GATGGAATTA GAACTTGGCA AAACAATACT GAGAATGAAG   181 TGTATGTGGA ACAGAGGCTG CTGATCTCGT TCTTCAGGCT ATGAAACTGA CACATTTGGA   241 AACCACAGTA CTTAGAACCA CAAAGTGGGA ATCAAGAGAA AAACAATGAT CCCACGAGAG   301 ATCTATAGAT CTATAGATCA TGAGTGGGAG GAATGAGCTG GCCCTTAATT TGGTTTTGCT   361 TGTTTAAATT ATGATATNNN NNTATGAAAC ATTATCATAA AGCAATANNA NAGAGCCTTC   421 AGTAAAGANC NNGCATTNNN NNAATCCCAC CCCACCCCCA CCCCCGTAGC TCCAATCCTT   481 CCATTCAAAA TGTAGGTNNN NNNTTCTCAC CCTTCNTANC AAAGTANGAN NNGAAAAACT 541 TNNNNTTTAG TGNACNTCTT TATTGTTTAA TNNNNNNNNN N //
Primer: PGK_N_rev (Pr0243) Region: insert mid(loxP,SV40 pA,mClover 3') Sequence file: RDB13922_A6B8g.seq
>D03070A06_A6B8_1_PGK-N-rev_B06_06.ab1     1 NNNNNNNNNN CNNNNNNGGN NNANTTCGAG GTCGAGATCC TAAGCTTGGC TGGACGTAAA    61 CTCCTCTTCA GACCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ATTAAGGGTT   121 ATTGAATATG ATCGACGCGT TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT   181 GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT   241 TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA   301 GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA   361 TTATGATCAG TTATCTAGAT TACTTGTACA GCTCGTCCAT GCCATGTGTA ATCCCGGCGG   421 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGCT   481 TGGACTGATG GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG   541 TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CTCAACGTTG TGGCGGATCT   601 TGAAGTTAGC CTTGATGCCG TTCTTCTGCT TGTCGGCCGT GATATAGACG TTGTGGCTGT   661 TGAAGTTGTA CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT   721 TCAGCTCGAT GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT   781 ANGTACCGTC GTCCTTGAAA GAGATGGTGC GCTCCTGGAC GTANCCTTCG GGCATGGCGG   841 ANTTGAANAA NTCGTGCTGC TTCATGTGGT CGGGGTANCG GCTGAAGCNN NCACGCCGTA   901 NCCNAANGNG GTCNCGANGG TGGGNCAGGG NACNGGNANC TTGNCNNNTG GNGNNNANGA 961 NNNTNNNGGT CANCNTNGNC NNNNGNNGNA TNGCCNNNNN CNNCNNNNGN NNNNNNNNN //
Primer: PGKpro_F (Pr0249) Region: insert mid(Bsr,mPGK ter) Sequence file: RDB13922_A6B8h.seq
>D03070A06_A6B8_1_PGK-proF_B07_04.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNTCCNGGC CTTTCGACCT GCATCCNTCT AGATCTCGAG    61 CAGCTGAAGC TTACCGCTAG CATGAAAACA TTTAACATTT CTCAACAAGA TCTAGAATTA   121 GTAGAAGTAG CGACAGAGAA GATTACAATG CTTTATGAGG ATAATAAACA TCATGTGGGA   181 GCGGCAATTC GTACGAAAAC AGGAGAAATC ATTTCGGCAG TACATATTGA AGCGTATATA   241 GGACGAGTAA CTGTTTGTGC AGAAGCCATT GCGATTGGTA GTGCAGTTTC GAATGGACAA   301 AAGGATTTTG ACACGATTGT AGCTGTTAGA CACCCTTATT CTGACGAAGT AGATAGAAGT   361 ATTCGAGTGG TAAGTCCTTG TGGTATGTGT AGGGAGTTGA TTTCAGACTA TGCACCAGAT   421 TGTTTTGTGT TAATAGAAAT GAATGGCAAG TTAGTCAAAA CTACGATTGA AGAACTCATT   481 CCACTCAAAT ATACCCGAAA TTAAGCTAGC CGCCCGCCCC ACGACCCGCA GCGCCCGACC   541 GAAAGGAGCG CACGACCCCA TGCATCGATG ATATCAGATC CCCGGGATGC AGAAATTGAT   601 GATCTATTAA ACAATAAAGA TGTCCACTAA AATGGAAGTT TTTCCTGTCA TACTTTGTTA   661 AGAAGGGTGA GAACAGAGTA CCTACATTTT GAATGGAAGG ANTGGAGCTA CGGGGGTGGG   721 GGTGGGGTGG GATTAGATAA ATGCCTGCTC TTTACTGAAN GNTCTTTACT ATTGCTTTAT   781 GATAATGTTT CATAGTTGGA TATCATAATT TAAACNAGCN AAACCAANTT NANNGNCNNG   841 CTNATTCCNC CCACTCANGA TCTATAGATC TATAGATCTC TCNNGGGATC ATTGNTTTTN   901 NTCTNGATTN CCANTTTGTG GNTCTAAGTA CTGNNGNNTT NNNAANGTGT CNNTTTNNNA 961 GCCNGANNNA CGNNNTNAGC NNNCNNNGNT NCNNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl