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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK279 (mClover-Bsr)

A plasmid for construction of C-terminal mClover tagging donors.

Catalog number RDB13922
Resource name pMK279 (mClover-Bsr)
Alternative name mClover-Bsr, 999
Clone info. A plasmid for construction of C-terminal mClover tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone.
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Kanemaki, Masato |
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External Database
Aequorea victoria GFP

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
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            手続きの概要は、「提供申込みについて[link]」をご覧ください。

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            RDB13922 pMK279 (mClover-Bsr) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pMK279 (mClover-Bsr) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13922).

            Reference section:

            Natsume, T., Kiyomitsu, T., Saga, Y., Kanemaki, M.T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


            Tips

            Featured content

            Featured content Auxin Inducible Degron (AID) System (English text)
            Featured content Auxin Inducible Degron (AID) System (Japanese text)
            Featured content Genome Editing (English text)
            Featured content Genome Editing (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13922_A6B8p1-3.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: M13_-40 (Pr0410)
            Region: T7 pro,insert 5'(Linker,mClover 5')
            Sequence file: RDB13922_A6B8e.seq check
            >D03070A06_A6B8_1_M13(-40)_C02_08.ab1
                1 NNNNNNNNNN NNNNNNNNNG NNTNNCNATA GGGCGAATTG GAGCTCGGAT CCGGTGCAGG
               61 CGCCGCTAGC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT
              121 CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTC CGCGGCGAGG GCGAGGGCGA
              181 TGCCACCAAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC
              241 CTGGCCCACC CTCGTGACCA CCTTCGGCTA CGGCGTGGCC TGCTTCAGCC GCTACCCCGA
              301 CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG
              361 CACCATCTCT TTCAAGGACG ACGGTACCTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG
              421 CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT
              481 CCTGGGGCAC AAGCTGGAGT ACAACTTCAA CAGCCACAAC GTCTATATCA CGGCCGACAA
              541 GCAGAAGAAC GGCATCAAGG CTAACTTCAA GATCCGCCAC AACGTTGAGG ACGGCAGCGT
              601 GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC
              661 CGACAACCAC TACCTGAGCC ATCAGTCCAA GCTGANCAAA GACCCCAACG AGAAGCGCGA
              721 TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGNTT ACACATGGCA TGGACGANCT
              781 GTACANGTAA TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG ANGTTTTACT
              841 NGCTTTNAAA AACCNCCCAC ACCNNCCCCT GAANCNGAAA NNTAAAATGA ATGNNANTGT
              901 TGNTGNTNAC TNGNTTANNG NANCTNANNA NGGNTACNAA NAAAGCNANA NCATCNCNAN
            961 TTTCNNNAAT NAANNNNTTT TTTNNNNNNN NNN
            //
            Primer: M13R (Pr0160)
            Region: insert 3'(loxP,mPGK ter)
            Sequence file: RDB13922_A6B8f.seq check
            >D03070A06_A6B8_1_M13R_C01_07.ab1
                1 NNNNNNNNNN NNNNNNNNNN NCNAANGGNN NNNNNNNCTG GGTANCGGAT CCTAGTGAAC
               61 CTCTTCGAGG GANCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ATTAAGGGTT
              121 CCGGATCTCG ACCAGCTTCT GATGGAATTA GAACTTGGCA AAACAATACT GAGAATGAAG
              181 TGTATGTGGA ACAGAGGCTG CTGATCTCGT TCTTCAGGCT ATGAAACTGA CACATTTGGA
              241 AACCACAGTA CTTAGAACCA CAAAGTGGGA ATCAAGAGAA AAACAATGAT CCCACGAGAG
              301 ATCTATAGAT CTATAGATCA TGAGTGGGAG GAATGAGCTG GCCCTTAATT TGGTTTTGCT
              361 TGTTTAAATT ATGATATNNN NNTATGAAAC ATTATCATAA AGCAATANNA NAGAGCCTTC
              421 AGTAAAGANC NNGCATTNNN NNAATCCCAC CCCACCCCCA CCCCCGTAGC TCCAATCCTT
              481 CCATTCAAAA TGTAGGTNNN NNNTTCTCAC CCTTCNTANC AAAGTANGAN NNGAAAAACT
            541 TNNNNTTTAG TGNACNTCTT TATTGTTTAA TNNNNNNNNN N
            //
            Primer: PGK_N_rev (Pr0243)
            Region: insert mid(loxP,SV40 pA,mClover 3')
            Sequence file: RDB13922_A6B8g.seq check
            >D03070A06_A6B8_1_PGK-N-rev_B06_06.ab1
                1 NNNNNNNNNN CNNNNNNGGN NNANTTCGAG GTCGAGATCC TAAGCTTGGC TGGACGTAAA
               61 CTCCTCTTCA GACCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ATTAAGGGTT
              121 ATTGAATATG ATCGACGCGT TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT
              181 GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT
              241 TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA
              301 GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA
              361 TTATGATCAG TTATCTAGAT TACTTGTACA GCTCGTCCAT GCCATGTGTA ATCCCGGCGG
              421 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGCT
              481 TGGACTGATG GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG
              541 TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CTCAACGTTG TGGCGGATCT
              601 TGAAGTTAGC CTTGATGCCG TTCTTCTGCT TGTCGGCCGT GATATAGACG TTGTGGCTGT
              661 TGAAGTTGTA CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT
              721 TCAGCTCGAT GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT
              781 ANGTACCGTC GTCCTTGAAA GAGATGGTGC GCTCCTGGAC GTANCCTTCG GGCATGGCGG
              841 ANTTGAANAA NTCGTGCTGC TTCATGTGGT CGGGGTANCG GCTGAAGCNN NCACGCCGTA
              901 NCCNAANGNG GTCNCGANGG TGGGNCAGGG NACNGGNANC TTGNCNNNTG GNGNNNANGA
            961 NNNTNNNGGT CANCNTNGNC NNNNGNNGNA TNGCCNNNNN CNNCNNNNGN NNNNNNNNN
            //
            Primer: PGKpro_F (Pr0249)
            Region: insert mid(Bsr,mPGK ter)
            Sequence file: RDB13922_A6B8h.seq check
            >D03070A06_A6B8_1_PGK-proF_B07_04.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNTCCNGGC CTTTCGACCT GCATCCNTCT AGATCTCGAG
               61 CAGCTGAAGC TTACCGCTAG CATGAAAACA TTTAACATTT CTCAACAAGA TCTAGAATTA
              121 GTAGAAGTAG CGACAGAGAA GATTACAATG CTTTATGAGG ATAATAAACA TCATGTGGGA
              181 GCGGCAATTC GTACGAAAAC AGGAGAAATC ATTTCGGCAG TACATATTGA AGCGTATATA
              241 GGACGAGTAA CTGTTTGTGC AGAAGCCATT GCGATTGGTA GTGCAGTTTC GAATGGACAA
              301 AAGGATTTTG ACACGATTGT AGCTGTTAGA CACCCTTATT CTGACGAAGT AGATAGAAGT
              361 ATTCGAGTGG TAAGTCCTTG TGGTATGTGT AGGGAGTTGA TTTCAGACTA TGCACCAGAT
              421 TGTTTTGTGT TAATAGAAAT GAATGGCAAG TTAGTCAAAA CTACGATTGA AGAACTCATT
              481 CCACTCAAAT ATACCCGAAA TTAAGCTAGC CGCCCGCCCC ACGACCCGCA GCGCCCGACC
              541 GAAAGGAGCG CACGACCCCA TGCATCGATG ATATCAGATC CCCGGGATGC AGAAATTGAT
              601 GATCTATTAA ACAATAAAGA TGTCCACTAA AATGGAAGTT TTTCCTGTCA TACTTTGTTA
              661 AGAAGGGTGA GAACAGAGTA CCTACATTTT GAATGGAAGG ANTGGAGCTA CGGGGGTGGG
              721 GGTGGGGTGG GATTAGATAA ATGCCTGCTC TTTACTGAAN GNTCTTTACT ATTGCTTTAT
              781 GATAATGTTT CATAGTTGGA TATCATAATT TAAACNAGCN AAACCAANTT NANNGNCNNG
              841 CTNATTCCNC CCACTCANGA TCTATAGATC TATAGATCTC TCNNGGGATC ATTGNTTTTN
              901 NTCTNGATTN CCANTTTGTG GNTCTAAGTA CTGNNGNNTT NNNAANGTGT CNNTTTNNNA
            961 GCCNGANNNA CGNNNTNAGC NNNCNNNGNT NCNNNNNNNN NN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP]

            2023.10.05

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