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pMK279 (mClover-Bsr)

A plasmid for construction of C-terminal mClover tagging donors.

Catalog number RDB13922
Resource name pMK279 (mClover-Bsr)
Alternative name mClover-Bsr, 999
Clone info. A plasmid for construction of C-terminal mClover tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone.
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor Kanemaki, Masato |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13922 pMK279 (mClover-Bsr) DNA solution

cDNA Request [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)
Featured content Genome Editing (English text)
Featured content Genome Editing (Japanese text)
Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

References

original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13922_A6B8p1_v2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 RDB13922_A6B8a_2.seq
>D03070A06_A6B8_1_M13(-40)_C02_08.ab1
    1 CGTCGGACGC CGCGTATACG ACTCACTATA GGGCGAATTG GAGCTCGGAT CCGGTGCAGG
   61 CGCCGCTAGC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT
  121 CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTC CGCGGCGAGG GCGAGGGCGA
  181 TGCCACCAAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC
  241 CTGGCCCACC CTCGTGACCA CCTTCGGCTA CGGCGTGGCC TGCTTCAGCC GCTACCCCGA
  301 CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG
  361 CACCATCTCT TTCAAGGACG ACGGTACCTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG
  421 CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT
  481 CCTGGGGCAC AAGCTGGAGT ACAACTTCAA CAGCCACAAC GTCTATATCA CGGCCGACAA
  541 GCAGAAGAAC GGCATCAAGG CTAACTTCAA GATCCGCCAC AACGTTGAGG ACGGCAGCGT
  601 GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC
  661 CGACAACCAC TACCTGAGCC ATCAGTCCAA GCTGAGCAAA GACCCCAACG AGAAGCGCGA
  721 TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATT ACACATGGCA TGGACGAGCT
  781 GTACAAGTAA TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT
  841 TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT
  901 TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA
  961 TTTCACAAAT AAAGCATTTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA
 1021 ATGTATCTTA ACGCGTCGAT CATATTCATT ACCCTAATAT AACTTCCGTA TATGTATGGC
 1081 TATACGGAGG TATAGTCTGA GAGAGTTAAC GTCCAGTCAA GCCTTAGTAT CCCTCGGAAC
1141 CCCTTCGA
//
Primer: PGKpro_F RDB13922_A6B8b_2.seq
>D03070A06_A6B8_1_PGK-proF_B07_04.ab1
    1 ATTGGGCGTT CTCTCTTCTC ATCTCCGGGC CTTTCGACCT GCATCCATCT AGATCTCGAG
   61 CAGCTGAAGC TTACCGCTAG CATGAAAACA TTTAACATTT CTCAACAAGA TCTAGAATTA
  121 GTAGAAGTAG CGACAGAGAA GATTACAATG CTTTATGAGG ATAATAAACA TCATGTGGGA
  181 GCGGCAATTC GTACGAAAAC AGGAGAAATC ATTTCGGCAG TACATATTGA AGCGTATATA
  241 GGACGAGTAA CTGTTTGTGC AGAAGCCATT GCGATTGGTA GTGCAGTTTC GAATGGACAA
  301 AAGGATTTTG ACACGATTGT AGCTGTTAGA CACCCTTATT CTGACGAAGT AGATAGAAGT
  361 ATTCGAGTGG TAAGTCCTTG TGGTATGTGT AGGGAGTTGA TTTCAGACTA TGCACCAGAT
  421 TGTTTTGTGT TAATAGAAAT GAATGGCAAG TTAGTCAAAA CTACGATTGA AGAACTCATT
  481 CCACTCAAAT ATACCCGAAA TTAAGCTAGC CGCCCGCCCC ACGACCCGCA GCGCCCGACC
  541 GAAAGGAGCG CACGACCCCA TGCATCGATG ATATCAGATC CCCGGGATGC AGAAATTGAT
  601 GATCTATTAA ACAATAAAGA TGTCCACTAA AATGGAAGTT TTTCCTGTCA TACTTTGTTA
  661 AGAAGGGTGA GAACAGAGTA CCTACATTTT GAATGGAAGG ATTGGAGCTA CGGGGGTGGG
721 GGTGGGGTGG G
//
Primer: PGK_N_rev RDB13922_A6B8c_2.seq
>D03070A06_A6B8_1_PGK-N-rev_B06_06.ab1
    1 CGTGACGCTC CCTACCCGGT AGATTTCGAG GTCGAGATCC TAAGCTTGGC TGGACGTAAA
   61 CTCCTCTTCA GACCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ATTAAGGGTT
  121 ATTGAATATG ATCGACGCGT TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT
  181 GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT
  241 TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA
  301 GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA
  361 TTATGATCAG TTATCTAGAT TACTTGTACA GCTCGTCCAT GCCATGTGTA ATCCCGGCGG
  421 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGCT
  481 TGGACTGATG GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG
  541 TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CTCAACGTTG TGGCGGATCT
  601 TGAAGTTAGC CTTGATGCCG TTCTTCTGCT TGTCGGCCGT GATATAGACG TTGTGGCTGT
  661 TGAAGTTGTA CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT
  721 TCAGCTCGAT GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT
  781 AGGTACCGTC GTCCTTGAAA GAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG
  841 ACTTGAAGAA GTCGTGCTGC TTCATGTGGT CGGGGTAGCG GCTGAAGCAG CCACGCCGTA
  901 GCCGAAGGTG GTCACGAGGG TGGGCCAGGG CACGGGCAGC TTGCCCGGTG GTGCAGATGA
  961 ACTTCAGGGT CAGCTTTGCC GTTGGTGGCA TCGCCCTCGC CCTCGCGCGG ACGCTGAACT
 1021 TGTGGCCGTT TACGTCGCCG TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT
 1081 CTCGCCCTGC TCAACATGCT AGCGCGCTGC ACGATCGAGC TCATTCGCCT ATTGTTGGAA
1141 GTCGGTAAAT TAAACGACG
//
Primer: M13R RDB13922_A6B8d_2.seq
>D03070A06_A6B8_1_M13R_C01_07.ab1
    1 TGTCGCGGGC ATTAACCCTC ACTAAAGGGA ACAAAAGCTG GGTACCGGAT CCTAGTGAAC
   61 CTCTTCGAGG GACCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ATTAAGGGTT
  121 CCGGATCTCG ACCAGCTTCT GATGGAATTA GAACTTGGCA AAACAATACT GAGAATGAAG
  181 TGTATGTGGA ACAGAGGCTG CTGATCTCGT TCTTCAGGCT ATGAAACTGA CACATTTGGA
  241 AACCACAGTA CTTAGAACCA CAAAGTGGGA ATCAAGAGAA AAACAATGAT CCCACGAGAG
  301 ATCTATAGAT CTATAGATCA TGAGTGGGAG GAATGAGCTG GCCCTTAATT TGGTTTTGCT
  361 TGTTTAAATT ATGATATCCA ACTATGAAAC ATTATCATAA AGCAATAGTA AAGAGCCTTC
  421 AGTAAAGAGC AGGCATTTAT CTAATCCCAC CCCACCCCCA CCCCCGTAGC TCCAATCCTT
  481 CCATTCAAAA TGTAGGTACT CTGTTCTCAC CCTTCTTAAC AAAGTATGAC AGGAAAAACT
  541 TCCATTTTAG TGGACATCTT TATTGTTTAA TAGATCATCA ATTTCTGCAT CCCGGGGATC
  601 TGATATCATC GATGCATGGG GTCGTGCGCT CCTTTCGGTC GGGCGCTGCG GGTCGTGGGG
  661 CGGGCGGCTA GCTTAATTTC GGGTATATTT GAGTGGAATG AAGTTCTTCA ATCGTAGTTT
  721 TGACTAAATT GCCCATTCAT TTCTATTAAC ACAAAACAAT CTGGTGCATA GTCTGAAAAT
781 CAACTCCCTA CACATACCAC AAGGACTTTA CCACTCGAAA TACTTCTATC TACTT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

2018.12.11

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