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pMK377 (Hygro-P2A-mCherry2) (#RDB16815)

A plasmid for construction of N-terminal Hygro-P2A-mCherry2 tagging donors.

Clone info. A plasmid for construction of N-terminal Hygro-P2A-mCherry2 tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Growth conditions LB+Amp, 37oC
Gene/insert name Discosoma sp. RFP cDNA
Depositor|Developer Kanemaki, Masato |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16815 pMK377 (Hygro-P2A-mCherry2) DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMK377 (Hygro-P2A-mCherry2) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16815).

Reference section:

Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16815_A8Lpp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 ()
Sequence file: RDB16815_A8Lpa.seq check
>D06157B2_A8Lp_1_pUC-M13_-100_G07_19_ABI24.ab1
    1 GGAGGGTCAG GCGATTAGGT TGGGTACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC
   61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGGTA CCGGGCCCCC
  121 CCTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGCCACC ATGAAAAAGC CTGAACTCAC
  181 CGCGACGTCT GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA
  241 GCTCTCGGAG GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT
  301 CCTGCGGGTA AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT
  361 TGCATCGGCC GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT
  421 GACCTATTGC ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA
  481 ACTGCCCGCT GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT
  541 TAGCCAGACG AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG
  601 GCGTGATTTC ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA
  661 CGACACCGTC AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA
  721 CTGCCCCGAA GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG TCCTGACGGA
  781 CAATGGCCGC ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG ATTCCCAATA
  841 CGAGGTCGCC AACATCTTCT TCTGGAGGCC GTGGTTGGCT TGTATGGAGC AGCAGACGCG
  901 CTACTTCGAG CGGAGGCATC CGGAGCTTGC AGGATCGCCC GCGGCTCCCG GGCGTATATG
  961 CTCCGCATTG GGTCTTGACC CAACTCTATC AGAGCTTTGC ATGACGGCAA TTTCCGATGA
 1021 TGCCAGCCTT TGGCGCAGGA TTCGAATGCC GACGCATTCG TTCGATCCGC AGCCTGGTAC
1081 TGATCGGTCG TAACACAAAA A
//
Primer: reverse ()
Sequence file: RDB16815_A8Lpb.seq check
>D06157B2_A8LP_1_reverse_G04_19_ABI24.ab1
    1 CTGCCAATTA ACCTCACTAA AGGGAACAAA AGCTGGAGCT CCACCGCGGT GGCGGCCGCT
   61 CTAGAACTAG TGGATCCGGC GCCTGCACCA GCGCCCTTGT ACAGCTCGTC CATGCCGCCG
  121 GTGGAGTGGC GGCCCTCGGC GCGTTCGTAC TGTTCCACGA TGGTGTAGTC CTCGTTGTGG
  181 GAAAGGATGT CCAACTTGAT GTCGACGTTG TAGGCGCCGG GCAGCTGCAC GGGCTTCTTG
  241 GCCTTGTAGG TGGTCTTGAC CTCAGCGTCG TAGTGGCCGC CGTCCTTCAG CTTCAGCCTC
  301 TGCTTGATCT CGCCCTTCAG GGCGCCGTCC TCGGGGTACA TCCGCTCAGT GGAGGCCTCC
  361 CAGCCCATGG TACGACACTG CATTACGGGG CCGTCGGAGG GGAAGTTGGT GCCGCGCAGC
  421 TTCACCTTGT AGATGAACTC GCCGTCCTGC AGGGAGGAGT CCTGGGTCAC GGTCACCACG
  481 CCGCCGTCCT CGAAGTTCAT CACGCGCTCC CAATTGAAGC CCTCGGGGAA GGACAGCTTC
  541 AAGTAGTCGG GGATGTCGGC GGGGTGCTTC ACGTAGGCCT TGGAGCCGTA CATGAACTGA
  601 GGGGACAGGA TGTCCCAGGC GAAGGGCAGG GGGCCACCCT TGGTCACCTT CAGCTTGGCG
  661 GTCTGGGTGC CCTCGTAGGG GCGGCCCTCG CCCTCGCCCT CGATCTCGAA CTCGTGGCCG
  721 TTCACGGAGC CCTCCATGTG CACCTTGAAG CGCATGAACT CCTTGATGAT GGCCATGTTA
  781 TCCTCCTCGC CCTTGCTCAC CATAGATCTA GGTCCAGGGT TCTCCTCCAC GTCTCCAGCC
  841 TGCTTCAGCA GGCTGAAGTT AGTAGCTCCG CTTCCTGATT CCTTTGCCCT CGGACGAGTG
  901 CTGGGGGCGT CGGTTTCCAC TATCGGCGAG TACTTCTACA CAGCCATCGG TCCAGACGCC
  961 GCGCTTCTGC GGGCGATTTG TGTACGCCCG ACAGTCCCGG CTCCGGATCG GGACGATTGC
 1021 GTCGCATTCG ACCCTGCGCC CAAGCTGCAT CATCGAATGG CCGTCCAACC AAGCCTCTGG
1081 AATAAGAAG
//
Primer: HygR-C_F ()
Sequence file: RDB16815_A8Lpc.seq check
>D06157B2_A8LP_1_HygR-C_F_H04_22_ABI24.ab1
    1 CATTTTCGGG GGCATTCCCA ATACGAGGTC GCCAACATCT TCTTCTGGAG GCCGTGGTTG
   61 GCTTGTATGG AGCAGCAGAC GCGCTACTTC GAGCGGAGGC ATCCGGAGCT TGCAGGATCG
  121 CCGCGGCTCC GGGCGTATAT GCTCCGCATT GGTCTTGACC AACTCTATCA GAGCTTGGTT
  181 GACGGCAATT TCGATGATGC AGCTTGGGCG CAGGGTCGAT GCGACGCAAT CGTCCGATCC
  241 GGAGCCGGGA CTGTCGGGCG TACACAAATC GCCCGCAGAA GCGCGGCCGT CTGGACCGAT
  301 GGCTGTGTAG AAGTACTCGC CGATAGTGGA AACCGACGCC CCAGCACTCG TCCGAGGGCA
  361 AAGGAATCAG GAAGCGGAGC TACTAACTTC AGCCTGCTGA AGCAGGCTGG AGACGTGGAG
  421 GAGAACCCTG GACCTAGATC TATGGTGAGC AAGGGCGAGG AGGATAACAT GGCCATCATC
  481 AAGGAGTTCA TGCGCTTCAA GGTGCACATG GAGGGCTCCG TGAACGGCCA CGAGTTCGAG
  541 ATCGAGGGCG AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA GCTGAAGGTG
  601 ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCTCAGTT CATGTACGGC
  661 TCCAAGGCCT ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACTTGAAGCT GTCCTTCCCC
  721 GAGGGCTTCA ATTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT GACCGTGACC
  781 CAGGACTCCT CCCTGCAGGA CGGCGAGTTC ATCTACAAGG TGAAGCTGCG CGGCACCAAC
  841 TTCCCCTCCG ACGGCCCCGT AATGCAGTGT CGTACCATGG GGCTGGGAGG CCTCCACTGA
  901 GCGGATGTAC CCCGAGGACG GCGCCCTGAA GGGCGAGATC AAGCAGAGGC TGAAGCTGAT
  961 GGACGGCGGG CCACTACGAC GCTGAGGTCA GACCACCTAC AAGGCCCAAG AGGCCCGTGC
 1021 AGCTGCCCGG GCGTCTACAC CGTCGACATC CAAGTTGCAC ATCGTTCCCA CAACGAAGGA
1081 ACTACCACCA ATTCG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]