Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK377 (Hygro-P2A-mCherry2)

A plasmid for construction of N-terminal Hygro-P2A-mCherry2 tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	提供依頼書 [Word] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16815_A8Lpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 () Sequence file: RDB16815_A8Lpa.seq
>D06157B2_A8Lp_1_pUC-M13_-100_G07_19_ABI24.ab1     1 GGAGGGTCAG GCGATTAGGT TGGGTACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC    61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGGTA CCGGGCCCCC   121 CCTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGCCACC ATGAAAAAGC CTGAACTCAC   181 CGCGACGTCT GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA   241 GCTCTCGGAG GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT   301 CCTGCGGGTA AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT   361 TGCATCGGCC GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT   421 GACCTATTGC ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA   481 ACTGCCCGCT GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT   541 TAGCCAGACG AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG   601 GCGTGATTTC ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA   661 CGACACCGTC AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA   721 CTGCCCCGAA GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG TCCTGACGGA   781 CAATGGCCGC ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG ATTCCCAATA   841 CGAGGTCGCC AACATCTTCT TCTGGAGGCC GTGGTTGGCT TGTATGGAGC AGCAGACGCG   901 CTACTTCGAG CGGAGGCATC CGGAGCTTGC AGGATCGCCC GCGGCTCCCG GGCGTATATG   961 CTCCGCATTG GGTCTTGACC CAACTCTATC AGAGCTTTGC ATGACGGCAA TTTCCGATGA  1021 TGCCAGCCTT TGGCGCAGGA TTCGAATGCC GACGCATTCG TTCGATCCGC AGCCTGGTAC 1081 TGATCGGTCG TAACACAAAA A //
Primer: reverse () Sequence file: RDB16815_A8Lpb.seq
>D06157B2_A8LP_1_reverse_G04_19_ABI24.ab1     1 CTGCCAATTA ACCTCACTAA AGGGAACAAA AGCTGGAGCT CCACCGCGGT GGCGGCCGCT    61 CTAGAACTAG TGGATCCGGC GCCTGCACCA GCGCCCTTGT ACAGCTCGTC CATGCCGCCG   121 GTGGAGTGGC GGCCCTCGGC GCGTTCGTAC TGTTCCACGA TGGTGTAGTC CTCGTTGTGG   181 GAAAGGATGT CCAACTTGAT GTCGACGTTG TAGGCGCCGG GCAGCTGCAC GGGCTTCTTG   241 GCCTTGTAGG TGGTCTTGAC CTCAGCGTCG TAGTGGCCGC CGTCCTTCAG CTTCAGCCTC   301 TGCTTGATCT CGCCCTTCAG GGCGCCGTCC TCGGGGTACA TCCGCTCAGT GGAGGCCTCC   361 CAGCCCATGG TACGACACTG CATTACGGGG CCGTCGGAGG GGAAGTTGGT GCCGCGCAGC   421 TTCACCTTGT AGATGAACTC GCCGTCCTGC AGGGAGGAGT CCTGGGTCAC GGTCACCACG   481 CCGCCGTCCT CGAAGTTCAT CACGCGCTCC CAATTGAAGC CCTCGGGGAA GGACAGCTTC   541 AAGTAGTCGG GGATGTCGGC GGGGTGCTTC ACGTAGGCCT TGGAGCCGTA CATGAACTGA   601 GGGGACAGGA TGTCCCAGGC GAAGGGCAGG GGGCCACCCT TGGTCACCTT CAGCTTGGCG   661 GTCTGGGTGC CCTCGTAGGG GCGGCCCTCG CCCTCGCCCT CGATCTCGAA CTCGTGGCCG   721 TTCACGGAGC CCTCCATGTG CACCTTGAAG CGCATGAACT CCTTGATGAT GGCCATGTTA   781 TCCTCCTCGC CCTTGCTCAC CATAGATCTA GGTCCAGGGT TCTCCTCCAC GTCTCCAGCC   841 TGCTTCAGCA GGCTGAAGTT AGTAGCTCCG CTTCCTGATT CCTTTGCCCT CGGACGAGTG   901 CTGGGGGCGT CGGTTTCCAC TATCGGCGAG TACTTCTACA CAGCCATCGG TCCAGACGCC   961 GCGCTTCTGC GGGCGATTTG TGTACGCCCG ACAGTCCCGG CTCCGGATCG GGACGATTGC  1021 GTCGCATTCG ACCCTGCGCC CAAGCTGCAT CATCGAATGG CCGTCCAACC AAGCCTCTGG 1081 AATAAGAAG //
Primer: HygR-C_F () Sequence file: RDB16815_A8Lpc.seq
>D06157B2_A8LP_1_HygR-C_F_H04_22_ABI24.ab1     1 CATTTTCGGG GGCATTCCCA ATACGAGGTC GCCAACATCT TCTTCTGGAG GCCGTGGTTG    61 GCTTGTATGG AGCAGCAGAC GCGCTACTTC GAGCGGAGGC ATCCGGAGCT TGCAGGATCG   121 CCGCGGCTCC GGGCGTATAT GCTCCGCATT GGTCTTGACC AACTCTATCA GAGCTTGGTT   181 GACGGCAATT TCGATGATGC AGCTTGGGCG CAGGGTCGAT GCGACGCAAT CGTCCGATCC   241 GGAGCCGGGA CTGTCGGGCG TACACAAATC GCCCGCAGAA GCGCGGCCGT CTGGACCGAT   301 GGCTGTGTAG AAGTACTCGC CGATAGTGGA AACCGACGCC CCAGCACTCG TCCGAGGGCA   361 AAGGAATCAG GAAGCGGAGC TACTAACTTC AGCCTGCTGA AGCAGGCTGG AGACGTGGAG   421 GAGAACCCTG GACCTAGATC TATGGTGAGC AAGGGCGAGG AGGATAACAT GGCCATCATC   481 AAGGAGTTCA TGCGCTTCAA GGTGCACATG GAGGGCTCCG TGAACGGCCA CGAGTTCGAG   541 ATCGAGGGCG AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA GCTGAAGGTG   601 ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCTCAGTT CATGTACGGC   661 TCCAAGGCCT ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACTTGAAGCT GTCCTTCCCC   721 GAGGGCTTCA ATTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT GACCGTGACC   781 CAGGACTCCT CCCTGCAGGA CGGCGAGTTC ATCTACAAGG TGAAGCTGCG CGGCACCAAC   841 TTCCCCTCCG ACGGCCCCGT AATGCAGTGT CGTACCATGG GGCTGGGAGG CCTCCACTGA   901 GCGGATGTAC CCCGAGGACG GCGCCCTGAA GGGCGAGATC AAGCAGAGGC TGAAGCTGAT   961 GGACGGCGGG CCACTACGAC GCTGAGGTCA GACCACCTAC AAGGCCCAAG AGGCCCGTGC  1021 AGCTGCCCGG GCGTCTACAC CGTCGACATC CAAGTTGCAC ATCGTTCCCA CAACGAAGGA 1081 ACTACCACCA ATTCG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.12.13

GNP_filter3_RDBDEP_html_231213.pl