Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK294 (mAID-mCherry2-Bsr)

A plasmid for construction of C-terminal mAID-mCherry tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Please contact us to request the Exclusive MTA.
Remarks	This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。
備考	Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13937_A6B8p1_v2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB13937_A6B8a_2.seq
>D03070A21_A6B8_1_M13(-40)_F05_17.ab1     1 AAGCCGGCGC GCGCGTATAC GACTCACTAT AGGGCGAATT GGAGCTCCCC GGGATCCGGT    61 GCAGGCGCCA AGGAGAAGAG TGCTTGTCCT AAAGATCCAG CCAAACCTCC GGCCAAGGCA   121 CAAGTTGTGG GATGGCCACC GGTGAGATCA TACCGGAAGA ACGTGATGGT TTCCTGCCAA   181 AAATCAAGCG GTGGCCCGGA GGCGGCGGCG TTCGTGAAGG TATCAATGGA CGGAGCACCG   241 TACTTGAGGA AAATCGATTT GAGGATGTAT AAAGCTAGCA TGGTGAGCAA GGGCGAGGAG   301 GATAACATGG CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA GGGCTCCGTG   361 AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA GGGCACCCAG   421 ACCGCCAAGC TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT TCGCCTGGGA CATCCTGTCC   481 CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT CCCCGACTAC   541 TTGAAGCTGT CCTTCCCCGA GGGCTTCAAT TGGGAGCGCG TGATGAACTT CGAGGACGGC   601 GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG GCGAGTTCAT CTACAAGGTG   661 AAGCTGCGCG GCACCAAACT TCCCCTCCGA CGGCCCCGTA ATGCAGTGTC GTACCATGGG   721 CTGGGAGGCC TCCACTGAGC GGATGTACCC CGAGGACGGC GCCCTGAAGG GCGAGATCAA   781 GCAGAGGCTG AAGCTGAAGA CGGCGGCCAC TACGACGCTG AGGTCAAGAC CACCTACAAG   841 GGCCAAGAAG CCCGTGCAGC TGCCCGGCGC CTACAACGTC GACATCAAGT TGGACATCCT   901 TTTCCCACAA CGAGGACTAC ACCATCGTGG AACAGTACGA ACGCGCCCGA GGGGCCGCCA   961 ACTCCACCGG GCGGCATGGG ACGAAGCTGT ACCAAGGTAA TCCTAGATAA CTGAATCATA  1021 ATTCAGCATA CCACATTTGG TAGAGGGTTT TACTTGCCTT TAAAAACCTT CCACACCTTC  1081 CCCCCTGAAC CTGAAACATA AAATGATTGC AATTGAGTGT ACATGGTTAA TGCAGCTATA 1141 AATGTACGAT AAAGCAGTAG AC //
Primer: PGK_N_rev Sequence file: RDB13937_A6B8b_2.seq
>D03070A21_A6B8_1_PGK-N-rev_H06_24.ab1     1 CATGACGCTC CCTACCGGTA GATTTCGAGG TCGAGATCCT AAGCTTGGCT GGACGTAAAC    61 TCCTCTTCAG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA TTAAGGGTTA   121 TTGAATATGA TCGACGCGTT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG   181 CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT   241 ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG   301 GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TATGGCTGAT   361 TATGATCAGT TATCTAGATT ACTTGTACAG CTCGTCCATG CCGCCGGTGG AGTGGCGGCC   421 CTCGGCGCGT TCGTACTGTT CCACGATGGT GTAGTCCTCG TTGTGGGAAA GGATGTCCAA   481 CTTGATGTCG ACGTTGTAGG CGCCGGGCAG CTGCACGGGC TTCTTGGCCT TGTAGGTGGT   541 CTTGACCTCA GCGTCGTAGT GGCCGCCGTC CTTCAGCTTC AGCCTCTGCT TGATCTCGCC   601 CTTCAGGGCG CCGTCCTCGG GGTACATCCG CTCAGTGGAG GCCTCCCAGC CCATGGTACG   661 ACACTGCATT ACGGGGCCGT CGGAGGGGAA GTTGGTGCCG CGCAGCTTCA CCTTGTAGAT   721 GAACTCGCCG TCCTGCAGGG AGGAGTCCTG GGTCACGGTC ACCACGCCGC CGTCCTCGAA   781 GTTCATCACG CGCTCCCAAT TGAAGCCCTC GGGGAAGGAC AGCTTCAAGT AGTCGGGGAT   841 GTCGGCGGGG TGCTTCACGT AGGCCTTGGA GCCGTACATG AACTGAGGGG ACAGGATGTC   901 CCAGGCGAAG GGCAGGGGGC CACCCTTGGT CACCTTCAGC TTGGCGGTCT GGGTGCCCTC   961 GTAGGGCGGC CCTCGCCCTC GCCTCGATCT CGAACTCGTG GCCGTTCACG GAGCCCTCAA  1021 TGTGCCACCT TGAAGCGCAT GAACTCCTGA TGATGCCCAT GTTATCCTCC TCGCCTGCTC  1081 ACAATGCTAG CTTAACATCT CATCGATTCC TTCAGTACGT GCCTCGGTTC CCATTGAATA 1141 CCCTTTTCA //
Primer: PGKpro_F Sequence file: RDB13937_A6B8c_2.seq
>D03070A21_A6B8_1_PGK-proF_C08_08.ab1     1 ATCGGCGTAT CTCTCTTCTC ATCTCCGGGC CTTTCGACCT GCATCCATCT AGATCTCGAG    61 CAGCTGAAGC TTACCGCTAG CATGAAAACA TTTAACATTT CTCAACAAGA TCTAGAATTA   121 GTAGAAGTAG CGACAGAGAA GATTACAATG CTTTATGAGG ATAATAAACA TCATGTGGGA   181 GCGGCAATTC GTACGAAAAC AGGAGAAATC ATTTCGGCAG TACATATTGA AGCGTATATA   241 GGACGAGTAA CTGTTTGTGC AGAAGCCATT GCGATTGGTA GTGCAGTTTC GAATGGACAA   301 AAGGATTTTG ACACGATTGT AGCTGTTAGA CACCCTTATT CTGACGAAGT AGATAGAAGT   361 ATTCGAGTGG TAAGTCCTTG TGGTATGTGT AGGGAGTTGA TTTCAGACTA TGCACCAGAT   421 TGTTTTGTGT TAATAGAAAT GAATGGCAAG TTAGTCAAAA CTACGATTGA AGAACTCATT   481 CCACTCAAAT ATACCCGAAA TTAAGCTAGC CGCCCGCCCC ACGACCCGCA GCGCCCGACC   541 GAAAGGAGCG CACGACCCCA TGCATCGATG ATATCAGATC CCCGGGATGC AGAAATTGAT   601 GATCTATTAA ACAATAAAGA TGTCCACTAA AATGGAAGTT TTTCCTGTCA TACTTTGTTA   661 AGAAGGGTGA GAACAGAGTA CCTACATTTT GAATGGAAGG ATTGGAGCTA CGGGGGTGGG   721 GGTGGGGTGG GATTAGATAA ATGCCTGCTC TTTACTGAAG GCTCTTTACT ATTGCTTTAT   781 GATAATGTTT CATAGTTGGA TATCATAATT TAAACAAGCA AAACCAAATT AAAGGGCCAG   841 CTCATTCCTC CCACTCATGA TCTATAGATC TATAGATCTC TCGTGGGATC ATTGTTTTTC   901 TCTTGATTCC CACTTTGTGG TTCTAAGTAC TGTGGTTTCC CAAATGTGTC AGTTTCATAG   961 CCTGAAAGAA CGAGATCAGC AAGCCCTCTG TTCCACATAC ACTTCATTCT CAAGTATTGT  1021 TTTGCCCAAA GTTCTAATTT CCATCAGAAG CTGGGTCGAG GATCCGGAAC CCCTTAAATA  1081 TTACCTTTCG TATAATGTAA TGCCTAATAC GAAGTATTAG GTCCCTCGAG AAGGTTTCAC 1141 TAAGGATTCG GGTTACCCAA GGCATTTGTA GTA //
Primer: M13R Sequence file: RDB13937_A6B8d_2.seq
>D03070A21_A6B8_1_M13R_F04_16.ab1     1 CTCGCCGCAG TAACCCTCAC TAAAGGGAAC AAAAGCTGGG TACCGGATCC TAGTGAACCT    61 CTTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC GAAGTTATAT TAAGGGTTCC   121 GGATCTCGAC CAGCTTCTGA TGGAATTAGA ACTTGGCAAA ACAATACTGA GAATGAAGTG   181 TATGTGGAAC AGAGGCTGCT GATCTCGTTC TTCAGGCTAT GAAACTGACA CATTTGGAAA   241 CCACAGTACT TAGAACCACA AAGTGGGAAT CAAGAGAAAA ACAATGATCC CACGAGAGAT   301 CTATAGATCT ATAGATCATG AGTGGGAGGA ATGAGCTGGC CCTTAATTTG GTTTTGCTTG   361 TTTAAATTAT GATATCCAAC TATGAAACAT TATCATAAAG CAATAGTAAA GAGCCTTCAG   421 TAAAGAGCAG GCATTTATCT AATCCCACCC CACCCCCACC CCCGTAGCTC CAATCCTTCC   481 ATTCAAAATG TAGGTACTCT GTTCTCACCC TTCTTAACAA AGTATGACAG GAAAAACTTC   541 CATTTTAGTG GACATCTTTA TTGTTTAATA GATCATCAAT TTCTGCATCC CGGGGATCTG   601 ATATCATCGA TGCATGGGGT CGTGCGCTCC TTTCGGTCGG GCGCTGCGGG TCGTGGGGCG   661 GGCGGCTAGC TTAATTTCGG GTATATTTGA GTGGAATGAG TTCTTCAATC GTAGTTTTGA   721 CTAACTTGCC ATTCATTTCT ATTAACACAA AACAATCTGG TGCATAGTCT GAAATCAACT   781 CCCTACACAT ACCACAAGGA CTTACCACTC GAATACTTCT ATCTACTTCG TCAGAATAAG   841 GTGTCTAACA GCTACAATCG TGTCAAAATC CTTTTGTCCA TTCGAAACTG CACTACCAAT   901 CGCAATGGCT TCTGCACAAA CAGTTACTCG TCCTATAAAA CGCCTTCAAT ATGTACTGCC   961 CGAAATGAAT TTCTCCCTGT TTTCGTACGA AATTGCCGCT CCCACATGAA TGTTTAATTA  1021 TCCTCATAAG GCCATTGTAA TCTTCTCTTG TCGGCTACTT CTACATATTT CTAGAATCCT  1081 GTTGAGAAAT GTTAATGTTT TTTACATGCT AGCCGGTAAG CTCCAGGCTT GCCTTCGAGG 1141 ATTCTATAAA TTGGGAAATG CACAAGGATT CGCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.02.27

GNP_filter3_RDBDEP_html_230227.pl