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pMK294 (mAID-mCherry2-Bsr)

A plasmid for construction of C-terminal mAID-mCherry tagging donors.

Catalog number RDB13937
Resource name pMK294 (mAID-mCherry2-Bsr)
Alternative name mAID-mCherry2-Bsr, 976
Clone info. A plasmid for construction of C-terminal mAID-mCherry tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone.
To make 0.5 M stock solution, dissolve indole-3-acetic acid (IAA) (Sigma-Aldrich, 45533) or 1-naphthaleneacetic acid (NAA) (Sigma-Aldrich, 35745) in 100 % DMSO. IAA solution can be made in 100 % ethanol. Aliquots can be kept for 6 months in the dark at -20oC (Devrekanli, A., Kanemaki, M.T. Methods in Molecular Biology, 1369, 257-278, 2016).
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Discosoma sp. RFP cDNA
Depositor Kanemaki, Masato |
 
Other clones in our bank Arabidopsis thaliana AtIAA17 (KEGG orthology K14484) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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RDB13937 pMK294 (mAID-mCherry2-Bsr) DNA solution

cDNA Request [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)

References

original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13937_A6B8p1_v2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 RDB13937_A6B8a_2.seq
>D03070A21_A6B8_1_M13(-40)_F05_17.ab1
    1 AAGCCGGCGC GCGCGTATAC GACTCACTAT AGGGCGAATT GGAGCTCCCC GGGATCCGGT
   61 GCAGGCGCCA AGGAGAAGAG TGCTTGTCCT AAAGATCCAG CCAAACCTCC GGCCAAGGCA
  121 CAAGTTGTGG GATGGCCACC GGTGAGATCA TACCGGAAGA ACGTGATGGT TTCCTGCCAA
  181 AAATCAAGCG GTGGCCCGGA GGCGGCGGCG TTCGTGAAGG TATCAATGGA CGGAGCACCG
  241 TACTTGAGGA AAATCGATTT GAGGATGTAT AAAGCTAGCA TGGTGAGCAA GGGCGAGGAG
  301 GATAACATGG CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA GGGCTCCGTG
  361 AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA GGGCACCCAG
  421 ACCGCCAAGC TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT TCGCCTGGGA CATCCTGTCC
  481 CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT CCCCGACTAC
  541 TTGAAGCTGT CCTTCCCCGA GGGCTTCAAT TGGGAGCGCG TGATGAACTT CGAGGACGGC
  601 GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG GCGAGTTCAT CTACAAGGTG
  661 AAGCTGCGCG GCACCAAACT TCCCCTCCGA CGGCCCCGTA ATGCAGTGTC GTACCATGGG
  721 CTGGGAGGCC TCCACTGAGC GGATGTACCC CGAGGACGGC GCCCTGAAGG GCGAGATCAA
  781 GCAGAGGCTG AAGCTGAAGA CGGCGGCCAC TACGACGCTG AGGTCAAGAC CACCTACAAG
  841 GGCCAAGAAG CCCGTGCAGC TGCCCGGCGC CTACAACGTC GACATCAAGT TGGACATCCT
  901 TTTCCCACAA CGAGGACTAC ACCATCGTGG AACAGTACGA ACGCGCCCGA GGGGCCGCCA
  961 ACTCCACCGG GCGGCATGGG ACGAAGCTGT ACCAAGGTAA TCCTAGATAA CTGAATCATA
 1021 ATTCAGCATA CCACATTTGG TAGAGGGTTT TACTTGCCTT TAAAAACCTT CCACACCTTC
 1081 CCCCCTGAAC CTGAAACATA AAATGATTGC AATTGAGTGT ACATGGTTAA TGCAGCTATA
1141 AATGTACGAT AAAGCAGTAG AC
//
Primer: PGK_N_rev RDB13937_A6B8b_2.seq
>D03070A21_A6B8_1_PGK-N-rev_H06_24.ab1
    1 CATGACGCTC CCTACCGGTA GATTTCGAGG TCGAGATCCT AAGCTTGGCT GGACGTAAAC
   61 TCCTCTTCAG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA TTAAGGGTTA
  121 TTGAATATGA TCGACGCGTT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG
  181 CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT
  241 ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG
  301 GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TATGGCTGAT
  361 TATGATCAGT TATCTAGATT ACTTGTACAG CTCGTCCATG CCGCCGGTGG AGTGGCGGCC
  421 CTCGGCGCGT TCGTACTGTT CCACGATGGT GTAGTCCTCG TTGTGGGAAA GGATGTCCAA
  481 CTTGATGTCG ACGTTGTAGG CGCCGGGCAG CTGCACGGGC TTCTTGGCCT TGTAGGTGGT
  541 CTTGACCTCA GCGTCGTAGT GGCCGCCGTC CTTCAGCTTC AGCCTCTGCT TGATCTCGCC
  601 CTTCAGGGCG CCGTCCTCGG GGTACATCCG CTCAGTGGAG GCCTCCCAGC CCATGGTACG
  661 ACACTGCATT ACGGGGCCGT CGGAGGGGAA GTTGGTGCCG CGCAGCTTCA CCTTGTAGAT
  721 GAACTCGCCG TCCTGCAGGG AGGAGTCCTG GGTCACGGTC ACCACGCCGC CGTCCTCGAA
  781 GTTCATCACG CGCTCCCAAT TGAAGCCCTC GGGGAAGGAC AGCTTCAAGT AGTCGGGGAT
  841 GTCGGCGGGG TGCTTCACGT AGGCCTTGGA GCCGTACATG AACTGAGGGG ACAGGATGTC
  901 CCAGGCGAAG GGCAGGGGGC CACCCTTGGT CACCTTCAGC TTGGCGGTCT GGGTGCCCTC
  961 GTAGGGCGGC CCTCGCCCTC GCCTCGATCT CGAACTCGTG GCCGTTCACG GAGCCCTCAA
 1021 TGTGCCACCT TGAAGCGCAT GAACTCCTGA TGATGCCCAT GTTATCCTCC TCGCCTGCTC
 1081 ACAATGCTAG CTTAACATCT CATCGATTCC TTCAGTACGT GCCTCGGTTC CCATTGAATA
1141 CCCTTTTCA
//
Primer: PGKpro_F RDB13937_A6B8c_2.seq
>D03070A21_A6B8_1_PGK-proF_C08_08.ab1
    1 ATCGGCGTAT CTCTCTTCTC ATCTCCGGGC CTTTCGACCT GCATCCATCT AGATCTCGAG
   61 CAGCTGAAGC TTACCGCTAG CATGAAAACA TTTAACATTT CTCAACAAGA TCTAGAATTA
  121 GTAGAAGTAG CGACAGAGAA GATTACAATG CTTTATGAGG ATAATAAACA TCATGTGGGA
  181 GCGGCAATTC GTACGAAAAC AGGAGAAATC ATTTCGGCAG TACATATTGA AGCGTATATA
  241 GGACGAGTAA CTGTTTGTGC AGAAGCCATT GCGATTGGTA GTGCAGTTTC GAATGGACAA
  301 AAGGATTTTG ACACGATTGT AGCTGTTAGA CACCCTTATT CTGACGAAGT AGATAGAAGT
  361 ATTCGAGTGG TAAGTCCTTG TGGTATGTGT AGGGAGTTGA TTTCAGACTA TGCACCAGAT
  421 TGTTTTGTGT TAATAGAAAT GAATGGCAAG TTAGTCAAAA CTACGATTGA AGAACTCATT
  481 CCACTCAAAT ATACCCGAAA TTAAGCTAGC CGCCCGCCCC ACGACCCGCA GCGCCCGACC
  541 GAAAGGAGCG CACGACCCCA TGCATCGATG ATATCAGATC CCCGGGATGC AGAAATTGAT
  601 GATCTATTAA ACAATAAAGA TGTCCACTAA AATGGAAGTT TTTCCTGTCA TACTTTGTTA
  661 AGAAGGGTGA GAACAGAGTA CCTACATTTT GAATGGAAGG ATTGGAGCTA CGGGGGTGGG
  721 GGTGGGGTGG GATTAGATAA ATGCCTGCTC TTTACTGAAG GCTCTTTACT ATTGCTTTAT
  781 GATAATGTTT CATAGTTGGA TATCATAATT TAAACAAGCA AAACCAAATT AAAGGGCCAG
  841 CTCATTCCTC CCACTCATGA TCTATAGATC TATAGATCTC TCGTGGGATC ATTGTTTTTC
  901 TCTTGATTCC CACTTTGTGG TTCTAAGTAC TGTGGTTTCC CAAATGTGTC AGTTTCATAG
  961 CCTGAAAGAA CGAGATCAGC AAGCCCTCTG TTCCACATAC ACTTCATTCT CAAGTATTGT
 1021 TTTGCCCAAA GTTCTAATTT CCATCAGAAG CTGGGTCGAG GATCCGGAAC CCCTTAAATA
 1081 TTACCTTTCG TATAATGTAA TGCCTAATAC GAAGTATTAG GTCCCTCGAG AAGGTTTCAC
1141 TAAGGATTCG GGTTACCCAA GGCATTTGTA GTA
//
Primer: M13R RDB13937_A6B8d_2.seq
>D03070A21_A6B8_1_M13R_F04_16.ab1
    1 CTCGCCGCAG TAACCCTCAC TAAAGGGAAC AAAAGCTGGG TACCGGATCC TAGTGAACCT
   61 CTTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC GAAGTTATAT TAAGGGTTCC
  121 GGATCTCGAC CAGCTTCTGA TGGAATTAGA ACTTGGCAAA ACAATACTGA GAATGAAGTG
  181 TATGTGGAAC AGAGGCTGCT GATCTCGTTC TTCAGGCTAT GAAACTGACA CATTTGGAAA
  241 CCACAGTACT TAGAACCACA AAGTGGGAAT CAAGAGAAAA ACAATGATCC CACGAGAGAT
  301 CTATAGATCT ATAGATCATG AGTGGGAGGA ATGAGCTGGC CCTTAATTTG GTTTTGCTTG
  361 TTTAAATTAT GATATCCAAC TATGAAACAT TATCATAAAG CAATAGTAAA GAGCCTTCAG
  421 TAAAGAGCAG GCATTTATCT AATCCCACCC CACCCCCACC CCCGTAGCTC CAATCCTTCC
  481 ATTCAAAATG TAGGTACTCT GTTCTCACCC TTCTTAACAA AGTATGACAG GAAAAACTTC
  541 CATTTTAGTG GACATCTTTA TTGTTTAATA GATCATCAAT TTCTGCATCC CGGGGATCTG
  601 ATATCATCGA TGCATGGGGT CGTGCGCTCC TTTCGGTCGG GCGCTGCGGG TCGTGGGGCG
  661 GGCGGCTAGC TTAATTTCGG GTATATTTGA GTGGAATGAG TTCTTCAATC GTAGTTTTGA
  721 CTAACTTGCC ATTCATTTCT ATTAACACAA AACAATCTGG TGCATAGTCT GAAATCAACT
  781 CCCTACACAT ACCACAAGGA CTTACCACTC GAATACTTCT ATCTACTTCG TCAGAATAAG
  841 GTGTCTAACA GCTACAATCG TGTCAAAATC CTTTTGTCCA TTCGAAACTG CACTACCAAT
  901 CGCAATGGCT TCTGCACAAA CAGTTACTCG TCCTATAAAA CGCCTTCAAT ATGTACTGCC
  961 CGAAATGAAT TTCTCCCTGT TTTCGTACGA AATTGCCGCT CCCACATGAA TGTTTAATTA
 1021 TCCTCATAAG GCCATTGTAA TCTTCTCTTG TCGGCTACTT CTACATATTT CTAGAATCCT
 1081 GTTGAGAAAT GTTAATGTTT TTTACATGCT AGCCGGTAAG CTCCAGGCTT GCCTTCGAGG
1141 ATTCTATAAA TTGGGAAATG CACAAGGATT CGCA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.
reference Devrekanli, A., Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes. Methods in Molecular Biology 1369: 257-278 (2016). PMID 26519318.
reference Nishimura, K., Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Curr. Protoc. Cell Biol. \64: 20.9.1-16 (2014). PMID 25181302 .

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl