Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK349 (BSD-P2A-mAID-mCherry2)

A plasmid for construction of N-terminal BSD-P2A-mAID-mCherry2 tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose)[Link]
Remarks	The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose).

Distribution information (domestic users) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 専用MTA (Takara Bio USA, Inc. FPs, 英語版のみ、非営利機関による非営利学術研究用)[Link]
備考	本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。専用MTA（Takara Bio USA, Inc. FPs, 英語版のみ、非営利機関による非営利学術研究用）をご参照ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16810_A8Lpp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 Region: T7 pro,MCS,insert5'(BSD,P2A,mAID,mCherry2 5') Sequence file: RDB16810_A8Lpa.seq
>D06157A6_A8LP_2_pUC-M13_-100_A03_03_ABI24.ab1     1 GAAGGGCAAG CGGATTAGTT GGGTACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG    61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGGTAC CGGGCCCCCC   121 CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGCCACCA TGGCCAAGCC TTTGTCTCAA   181 GAAGAATCCA CCCTCATTGA AAGAGCAACG GCTACAATCA ACAGCATCCC CATCTCTGAA   241 GACTACAGCG TCGCCAGCGC AGCTCTCTCT AGCGACGGCC GCATCTTCAC TGGTGTCAAT   301 GTATATCATT TTACTGGGGG ACCTTGTGCA GAACTCGTGG TGCTGGGCAC TGCTGCTGCT   361 GCGGCAGCTG GCAACCTGAC TTGTATCGTC GCGATCGGAA ATGAGAACAG GGGCATCTTG   421 AGCCCCTGCG GACGGTGCCG ACAGGTGCTT CTCGATCTGC ATCCTGGGAT CAAAGCCATA   481 GTGAAGGACA GTGATGGACA GCCGACGGCA GTTGGGATTC GTGAATTGCT GCCCTCTGGT   541 TATGTGTGGG AGGGCTCAGG AAGCGGAGCT ACTAACTTCA GCCTGCTGAA GCAGGCTGGA   601 GACGTGGAGG AGAACCCTGG ACCTAGATCT AAGGAGAAGA GTGCTTGTCC TAAAGATCCA   661 GCCAAACCTC CGGCCAAGGC ACAAGTTGTG GGATGGCCAC CGGTGAGATC ATACCGGAAG   721 AACGTGATGG TTTCCTGCCA AAAATCAAGC GGTGGCCCGG AGGCGGCGGC GTTCGTGAAG   781 GTATCAATGG ACGGAGCACC GTACTTGAGG AAAATCGATT TGAGGATGTA TAAAGCTAGC   841 ATGGTGAGCA AGGGCGAGGA GGATAACATG GCCATCATCA AAGGAGTTCA TGCGCTTCAA   901 GGGTGCACAT GGGAGGGCTC CGTGAAACGG GCCACGAGTT CGAGATCGAG GGCGAGGGCG   961 AGGGCCCGCC CCTACGAGGG CACCCAGACC CGCCCAAGCT GATGTGACCA AGGTGGCCCC  1021 TTGCCTTCGC TGGGACATCC TGGTCCTTCA GGTCATGTAC CGCTCCAGGT CCTACGTTGA 1081 AGCACCCTGG TCTGACATCC CTCTG //
Primer: reverse Region: T3 pro,MCS,insert3'(liker,mCherry2,mAID) Sequence file: RDB16810_A8Lpb.seq
>D06157A6_A8LP_2_reverse_B03_06_ABI24.ab1     1 ATTACAATTT ACCTCACTAA AGGGACAAAA GCTGGAGCTC CACCGCGGTG GCGGCCGCTC    61 TAGAACTAGT GGATCCGGCG CCTGCACCAG CGCCCTTGTA CAGCTCGTCC ATGCCGCCGG   121 TGGAGTGGCG GCCCTCGGCG CGTTCGTACT GTTCCACGAT GGTGTAGTCC TCGTTGTGGG   181 AAAGGATGTC CAACTTGATG TCGACGTTGT AGGCGCCGGG CAGCTGCACG GGCTTCTTGG   241 CCTTGTAGGT GGTCTTGACC TCAGCGTCGT AGTGGCCGCC GTCCTTCAGC TTCAGCCTCT   301 GCTTGATCTC GCCCTTCAGG GCGCCGTCCT CGGGGTACAT CCGCTCAGTG GAGGCCTCCC   361 AGCCCATGGT ACGACACTGC ATTACGGGGC CGTCGGAGGG GAAGTTGGTG CCGCGCAGCT   421 TCACCTTGTA GATGAACTCG CCGTCCTGCA GGGAGGAGTC CTGGGTCACG GTCACCACGC   481 CGCCGTCCTC GAAGTTCATC ACGCGCTCCC AATTGAAGCC CTCGGGGAAG GACAGCTTCA   541 AGTAGTCGGG GATGTCGGCG GGGTGCTTCA CGTAGGCCTT GGAGCCGTAC ATGAACTGAG   601 GGGACAGGAT GTCCCAGGCG AAGGGCAGGG GGCCACCCTT GGTCACCTTC AGCTTGGCGG   661 TCTGGGTGCC CTCGTAGGGG CGGCCCTCGC CCTCGCCCTC GATCTCGAAC TCGTGGCCGT   721 TCACGGAGCC CTCCATGTGC ACCTTGAAGC GCATGAACTC CTTGATGATG GCCATGTTAT   781 CCTCCTCGCC CTTGCTCACC ATGCTAGCTT TATACATCCT CAAATCGATT TTCCTCAAGT   841 ACGGTGCTCC GTCCATTGAT ACCTTCACGA ACGCCGCCGC CTCCGGGCCA CCGCTTGATT   901 TTTGGCAGGA AACCATCACG TTCTTCCGGT ATGATCTCAC CGGTGGGCCA TCCCACAACT   961 TGTGCCTTGG CCGGAGGTTT GGGCTGGGAT CTTTAGGACA AGCACTTCTT CTCTTTAGAT  1021 CTAGTCCAGG ATCTCTCAAC GTCTCCAGCC TGCTTCAGCA AGCCTGCAGG TTAGTTAGCT 1081 TCCCGGCC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2021.11.02

GNP_filter3_RDBDEP_html_211029.pl