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pMK349 (BSD-P2A-mAID-mCherry2) (#RDB16810)

A plasmid for construction of N-terminal BSD-P2A-mAID-mCherry2 tagging donors.

Clone info. A plasmid for construction of N-terminal BSD-P2A-mAID-mCherry2 tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Discosoma sp. RFP cDNA
Depositor|Developer Kanemaki, Masato |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。

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RDB16810 pMK349 (BSD-P2A-mAID-mCherry2) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMK349 (BSD-P2A-mAID-mCherry2) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16810).

Reference section:

Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16810_A8Lpp1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310)
Region: T7 pro,MCS,insert 5'(BSD,P2A,mAID 5')
Sequence file: RDB16810_A8Lpc.seq check
>D06157A6_A8LP_2_pUC-M13_-100_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGGNNNGCNN GGGTTTTCCN NNTCNCGACG TTGTAAAACG
   61 ACGGCCAGTG NGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGGTAC CGGGCCCCCC
  121 CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGCCACCA TGGCCAAGCC TTTGTCTCAA
  181 GAAGAATCCA CCCTCATTGA AAGAGCAACG GCTACAATCA ACAGCATCCC CATCTCTGAA
  241 GACTACAGCG TCGCCAGCGC AGCTCTCTCT AGCGACGGCC GCATCTTCAC TGGTGTCAAT
  301 GTATATCATT TTACTGGGGG ACCTTGTGCA GAACTCGTGG TGCTGGGCAC TGCTGCTGCT
  361 GCGGCAGCTG GCAACCTGAC TTGTATCGTC GCGATCGGAA ATGAGAACAG GGGCATCTTG
  421 AGCCCCTGCG GACGGTGCCG ACAGGTGCTT CTCGATCTGC ATCCTGGGAT CAAAGCCATA
  481 GTGAAGGACA GTGATGGACA GCCGACGGCA GTTGGGATTC GTGAATTGCT GCCCTCTGGT
  541 TATGTGTGGG AGGGCTCAGG AAGCGGAGCT ACTAACTTCA GCCTGCTGAA GCAGGCTGGA
  601 GACGTGGAGG AGAACCCTGG ACCTAGATCT AAGGAGAAGA GTGCTTGTCC TAAAGATCCA
  661 GCCAAACCTC CGGCCAAGGC ACAAGTTGTG GGATGGCCAC CGGTGAGATC ATACCGGAAG
  721 AACGTGATGG TTTCCTGCCN AAAATCAAGC GGTGGNCCGG ANGCGGCGGC GTTCGTGAAN
  781 GTATCANTGG ACGGNNCACC GTACTTGANG AAAATCGNTT TGANGATGTA TAAAGCTAGC
  841 ATNGTGAGCA AGGGCGANGN NGNNNACATG GNNNTCATNN ANNNANTTNA TGCNNTTNNA
  901 NGNNGCNNNN NGNNNGGNNN CNNGNANNNG NNNNNNANTT NNANNNNNNN GNNNANGNNN
961 NNGNNNNNNN NNN
//
Primer: reverse (Pr0099)
Region: MCS,insert 3'(liker,mCherry2 3')
Sequence file: RDB16810_A8Lpd.seq check
>D06157A6_A8LP_2_reverse_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNAA AGGGANNNAA GCTGGAGCTC CACCGCGGTG GCGGCCGCTC
   61 TAGAACTAGT GGATCCGGCG CCTGCACCAG CGCCCTTGTA CAGCTCGTCC ATGCCGCCGG
  121 TGGAGTGGCG GCCCTCGGCG CGTTCGTACT GTTCCACGAT GGTGTAGTCC TCGTTGTGGG
  181 AAAGGATGTC CAACTTGATG TCGACGTTGT AGGCGCCGGG CAGCTGCACG GGCTTCTTGG
  241 CCTTGTAGGT GGTCTTGACC TCAGCGTCGT AGTGGCCGCC GTCCTTCAGC TTCAGCCTCT
  301 GCTTGATCTC GCCCTTCAGG GCGCCGTCCT CGGGGTACAT CCGCTCAGTG GAGGCCTCCC
  361 AGCCCATGGT ACGACACTGC ATTACGGGGC CGTCGGAGGG GAAGTTGGTG CCGCGCAGCT
  421 TCACCTTGTA GATGAACTCG CCGTCCTGCA GGGAGGAGTC CTGGGTCACG GTCACCACGC
  481 CGCCGTCCTC GAAGTTCATC ACGCGCTCCC AATTGAAGCC CTCGGGGAAG GACAGCTTCA
  541 AGTAGTCGGG GATGTCGGCG GGGTGCTTCA CGTAGGCCTT GGAGCCGTAC ATGAACTGAG
  601 GGGACAGGAT GTCCCAGGCG AAGGGCAGGG GGCCACCCTT GGTCACCTTC AGCTTGGCGG
  661 TCTGGGTGCC CTCGTANGGG CGGCCCTCGC CCTCGCCCTC GATCTCGAAC TCGTGGCCGT
  721 TCACGGANCC CTCCATGTGC ACCTNGAAGC GCATGAACTC CTTGATGANG GCCATGTTAT
  781 CCTCCTCGCC CTTGCTCACC ATGCTAGCTT TATACATCCT CAAATCGATT TTCCTCNAGT
  841 ACGGNGCTCC NNCNATTGNT ACCTTCNCGA ACGCCGCCNC CTCNNGGCCA CCGCTNNANT
901 TTTGNNNNNA ANNNNTCACN NNCNNNNGGT ATGNNNNCNN NNNNNGNNNN NNNNNN
//
Primer: mCherry-check_R4 (Pr0527)
Region: insert mid(mCherry2 5',mAID,P2A,BSD 3')
Sequence file: RDB16810_A8Lpe.seq check
>D06157A6_A8LP_2_mCherry-check_R4_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG NNNNTNNNTG NACTGNNGGG NNAGGNNGTC CCAGGCGAAG
   61 GGCAGGGGGC CACCCTTGGT CACCTTCAGC TTGGCGGTCT GGGTGCCCTC GTAGGGGCGG
  121 CCCTCGCCCT CGCCCTCGAT CTCGAACTCG TGGCCGTTCA CGGAGCCCTC CATGTGCACC
  181 TTGAAGCGCA TGAACTCCTT GATGATGGCC ATGTTATCCT CCTCGCCCTT GCTCACCATG
  241 CTAGCTTTAT ACATCCTCAA ATCGATTTTC CTCAAGTACG GTGCTCCGTC CATTGATACC
  301 TTCACGAACG CCGCCGCCTC CGGGCCACCG CTTGATTTTT GGCAGGAAAC CATCACGTTC
  361 TTCCGGTATG ATCTCACCGG TGGCCATCCC ACAACTTGTG CCTTGGCCGG AGGTTTGGCT
  421 GGATCTTTAG GACAAGCACT CTTCTCCTTA GATCTAGGTC CAGGGTTCTC CTCCACGTCT
  481 CCAGCCTGCT TCAGCAGGCT GAAGTTAGTA GCTCCGCTTC CTGAGCCCTC CCACACATAA
  541 CCAGAGGGCA GCAATTCACG AATCCCAACT GCCGTCGGCT GTCCATCACT GTCCTTCACT
  601 ATGGCTTTGA TCCCAGGATG CAGATCGAGA AGCACCTGTC GGCACCGTCC GCAGGGGCTC
  661 AAGATGCCCC TGTTCTCATT TCCGATCGCG ACGATACAAG TCAGGTTGCC AGCTGCCGCA
  721 GCAGCAGCAG TGCCCAGCAC CACGAGTTCT GCACAANGTC CCCCAGTAAA ATGATATACA
  781 TTGACACCAG TGAAGANGCG GCCGTCGCTA GAGAGAGCTG CGCTGGCGAC GCTGTANNCT
  841 TCAGANATGG GGANGCTGTT GANTGTAGCC GTNGCTCTTT CNANGAGGGN GGANTCTTCT
  901 TGANANNAAN NNCTNNNNCN NNNGNGGNNA NATCNNNNNT ATCGATACCG NCNANNNNNN
961 NGGGGGGGNN CNNGNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]