Sequencing and PCR primers for primer information.
A plasmid for construction of N-terminal BSD-P2A-mAID-mCherry2 tagging donors.
| Catalog number | RDB16810 |
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| Resource name | pMK349 (BSD-P2A-mAID-mCherry2) |
| Clone info. | A plasmid for construction of N-terminal BSD-P2A-mAID-mCherry2 tagging donors. |
| Vector backbone | pBluescript (plasmid) |
| Size of vector backbone | 2.9 kb |
| Selectable markers | Am^r (E. coli) |
| Gene/insert name | Arabidopsis thaliana AtIAA17 cDNA Discosoma sp. RFP cDNA |
| Depositor | Kanemaki, Masato | |
| Other clones in our bank | Arabidopsis thaliana AXR3 (KEGG orthology K14484) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Exclusive MTA [Link] |
| Remarks | The BIOLOGICAL RESOURCE may not be used for COMMERCIAL PURPOSES. Please read the Exclusive MTA (Clontech FPs). |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB16810 | pMK349 (BSD-P2A-mAID-mCherry2) | DNA solution |
Electronic file
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Auxin Inducible Degron (AID) System (English text) |
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| Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB16810_A8Lpp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pUC-M13_-100 | RDB16810_A8Lpa.seq |
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>D06157A6_A8LP_2_pUC-M13_-100_A03_03_ABI24.ab1 1 GAAGGGCAAG CGGATTAGTT GGGTACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG 61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGGTAC CGGGCCCCCC 121 CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGCCACCA TGGCCAAGCC TTTGTCTCAA 181 GAAGAATCCA CCCTCATTGA AAGAGCAACG GCTACAATCA ACAGCATCCC CATCTCTGAA 241 GACTACAGCG TCGCCAGCGC AGCTCTCTCT AGCGACGGCC GCATCTTCAC TGGTGTCAAT 301 GTATATCATT TTACTGGGGG ACCTTGTGCA GAACTCGTGG TGCTGGGCAC TGCTGCTGCT 361 GCGGCAGCTG GCAACCTGAC TTGTATCGTC GCGATCGGAA ATGAGAACAG GGGCATCTTG 421 AGCCCCTGCG GACGGTGCCG ACAGGTGCTT CTCGATCTGC ATCCTGGGAT CAAAGCCATA 481 GTGAAGGACA GTGATGGACA GCCGACGGCA GTTGGGATTC GTGAATTGCT GCCCTCTGGT 541 TATGTGTGGG AGGGCTCAGG AAGCGGAGCT ACTAACTTCA GCCTGCTGAA GCAGGCTGGA 601 GACGTGGAGG AGAACCCTGG ACCTAGATCT AAGGAGAAGA GTGCTTGTCC TAAAGATCCA 661 GCCAAACCTC CGGCCAAGGC ACAAGTTGTG GGATGGCCAC CGGTGAGATC ATACCGGAAG 721 AACGTGATGG TTTCCTGCCA AAAATCAAGC GGTGGCCCGG AGGCGGCGGC GTTCGTGAAG 781 GTATCAATGG ACGGAGCACC GTACTTGAGG AAAATCGATT TGAGGATGTA TAAAGCTAGC 841 ATGGTGAGCA AGGGCGAGGA GGATAACATG GCCATCATCA AAGGAGTTCA TGCGCTTCAA 901 GGGTGCACAT GGGAGGGCTC CGTGAAACGG GCCACGAGTT CGAGATCGAG GGCGAGGGCG 961 AGGGCCCGCC CCTACGAGGG CACCCAGACC CGCCCAAGCT GATGTGACCA AGGTGGCCCC 1021 TTGCCTTCGC TGGGACATCC TGGTCCTTCA GGTCATGTAC CGCTCCAGGT CCTACGTTGA 1081 AGCACCCTGG TCTGACATCC CTCTG // |
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| Primer: reverse | RDB16810_A8Lpb.seq |
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>D06157A6_A8LP_2_reverse_B03_06_ABI24.ab1 1 ATTACAATTT ACCTCACTAA AGGGACAAAA GCTGGAGCTC CACCGCGGTG GCGGCCGCTC 61 TAGAACTAGT GGATCCGGCG CCTGCACCAG CGCCCTTGTA CAGCTCGTCC ATGCCGCCGG 121 TGGAGTGGCG GCCCTCGGCG CGTTCGTACT GTTCCACGAT GGTGTAGTCC TCGTTGTGGG 181 AAAGGATGTC CAACTTGATG TCGACGTTGT AGGCGCCGGG CAGCTGCACG GGCTTCTTGG 241 CCTTGTAGGT GGTCTTGACC TCAGCGTCGT AGTGGCCGCC GTCCTTCAGC TTCAGCCTCT 301 GCTTGATCTC GCCCTTCAGG GCGCCGTCCT CGGGGTACAT CCGCTCAGTG GAGGCCTCCC 361 AGCCCATGGT ACGACACTGC ATTACGGGGC CGTCGGAGGG GAAGTTGGTG CCGCGCAGCT 421 TCACCTTGTA GATGAACTCG CCGTCCTGCA GGGAGGAGTC CTGGGTCACG GTCACCACGC 481 CGCCGTCCTC GAAGTTCATC ACGCGCTCCC AATTGAAGCC CTCGGGGAAG GACAGCTTCA 541 AGTAGTCGGG GATGTCGGCG GGGTGCTTCA CGTAGGCCTT GGAGCCGTAC ATGAACTGAG 601 GGGACAGGAT GTCCCAGGCG AAGGGCAGGG GGCCACCCTT GGTCACCTTC AGCTTGGCGG 661 TCTGGGTGCC CTCGTAGGGG CGGCCCTCGC CCTCGCCCTC GATCTCGAAC TCGTGGCCGT 721 TCACGGAGCC CTCCATGTGC ACCTTGAAGC GCATGAACTC CTTGATGATG GCCATGTTAT 781 CCTCCTCGCC CTTGCTCACC ATGCTAGCTT TATACATCCT CAAATCGATT TTCCTCAAGT 841 ACGGTGCTCC GTCCATTGAT ACCTTCACGA ACGCCGCCGC CTCCGGGCCA CCGCTTGATT 901 TTTGGCAGGA AACCATCACG TTCTTCCGGT ATGATCTCAC CGGTGGGCCA TCCCACAACT 961 TGTGCCTTGG CCGGAGGTTT GGGCTGGGAT CTTTAGGACA AGCACTTCTT CTCTTTAGAT 1021 CTAGTCCAGG ATCTCTCAAC GTCTCCAGCC TGCTTCAGCA AGCCTGCAGG TTAGTTAGCT 1081 TCCCGGCC // |
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| Primer: mCherry-check_R4 | RDB16810_A8Lpc.seq |
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>D06157A6_A8LP_1_mCherry-check_R4_C03_09_ABI24.ab1 1 CTGGGGCTTA GTAGGCTTGG AGCGTACATG AACTGAGGGG ACAGGATGTC CCAGGCGAAG 61 GGCAGGGGGC CACCCTTGGT CACCTTCAGC TTGGCGGTCT GGGTGCCCTC GTAGGGGCGG 121 CCCTCGCCCT CGCCCTCGAT CTCGAACTCG TGGCCGTTCA CGGAGCCCTC CATGTGCACC 181 TTGAAGCGCA TGAACTCCTT GATGATGGCC ATGTTATCCT CCTCGCCCTT GCTCACCATG 241 CTAGCTTTAT ACATCCTCAA ATCGATTTTC CTCAAGTACG GTGCTCCGTC CATTGATACC 301 TTCACGAACG CCGCCGCCTC CGGGCCACCG CTTGATTTTT GGCAGGAAAC CATCACGTTC 361 TTCCGGTATG ATCTCACCGG TGGCCATCCC ACAACTTGTG CCTTGGCCGG AGGTTTGGCT 421 GGATCTTTAG GACAAGCACT CTTCTCCTTA GATCTAGGTC CAGGGTTCTC CTCCACGTCT 481 CCAGCCTGCT TCAGCAGGCT GAAGTTAGTA GCTCCGCTTC CTGAGCCCTC CCACACATAA 541 CCAGAGGGCA GCAATTCACG AATCCCAACT GCCGTCGGCT GTCCATCACT GTCCTTCACT 601 ATGGCTTTGA TCCCAGGATG CAGATCGAGA AGCACCTGTC GGCACCGTCC GCAGGGGCTC 661 AAGATGCCCC TGTTCTCATT TCCGATCGCG ACGATACAAG TCAGGTTGCC AGCTGCCGCA 721 GCAGCAGCAG TGCCCAGCAC CACGAGTTCT GCACAAGGTC CCCCAGTAAA ATGATATACA 781 TTGACACCAG TGAAGATGCG GCCGTCGCTA GAGAGAGCTG CGCTGGCGAC GCTGTAGTCT 841 TCAGAGATGG GGATGCTGTT GATTGTAGCC GTTGCTCTTT CAATGAGGGT GGATTCTTCT 901 TGAGACAAAA GGCTTGGCCC ATGGTGGCGA TATCAAGCTT ATCGATACCG TCGACCTCGA 961 GGGGGGGGGC CCGGGTACCC AATTCGCCCT ATAGTGAGTC GTATTTACGC GCGCTTCACT 1021 GGTCGTCGTT TTTACAACGT CGATGACCTG GGAAAACCCT GACGGTTAAC CAAACTTATT 1081 CGGCTTGGCC AGCACATCCC CCTTTTCGTC CAAGCTT // |
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Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
2019.07.12
GNP_filter3_RDBDEP_html_190431.pl