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pMK349 (BSD-P2A-mAID-mCherry2)

A plasmid for construction of N-terminal BSD-P2A-mAID-mCherry2 tagging donors.

Catalog number RDB16810
Resource name pMK349 (BSD-P2A-mAID-mCherry2)
Clone info. A plasmid for construction of N-terminal BSD-P2A-mAID-mCherry2 tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Discosoma sp. RFP cDNA
Depositor Kanemaki, Masato |
 
Other clones in our bank Arabidopsis thaliana AXR3 (KEGG orthology K14484) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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Remarks
The BIOLOGICAL RESOURCE may not be used for COMMERCIAL PURPOSES. Please read the Exclusive MTA (Clontech FPs).
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RDB16810 pMK349 (BSD-P2A-mAID-mCherry2) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16810_A8Lpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 RDB16810_A8Lpa.seq
>D06157A6_A8LP_2_pUC-M13_-100_A03_03_ABI24.ab1
    1 GAAGGGCAAG CGGATTAGTT GGGTACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG
   61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGGTAC CGGGCCCCCC
  121 CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGCCACCA TGGCCAAGCC TTTGTCTCAA
  181 GAAGAATCCA CCCTCATTGA AAGAGCAACG GCTACAATCA ACAGCATCCC CATCTCTGAA
  241 GACTACAGCG TCGCCAGCGC AGCTCTCTCT AGCGACGGCC GCATCTTCAC TGGTGTCAAT
  301 GTATATCATT TTACTGGGGG ACCTTGTGCA GAACTCGTGG TGCTGGGCAC TGCTGCTGCT
  361 GCGGCAGCTG GCAACCTGAC TTGTATCGTC GCGATCGGAA ATGAGAACAG GGGCATCTTG
  421 AGCCCCTGCG GACGGTGCCG ACAGGTGCTT CTCGATCTGC ATCCTGGGAT CAAAGCCATA
  481 GTGAAGGACA GTGATGGACA GCCGACGGCA GTTGGGATTC GTGAATTGCT GCCCTCTGGT
  541 TATGTGTGGG AGGGCTCAGG AAGCGGAGCT ACTAACTTCA GCCTGCTGAA GCAGGCTGGA
  601 GACGTGGAGG AGAACCCTGG ACCTAGATCT AAGGAGAAGA GTGCTTGTCC TAAAGATCCA
  661 GCCAAACCTC CGGCCAAGGC ACAAGTTGTG GGATGGCCAC CGGTGAGATC ATACCGGAAG
  721 AACGTGATGG TTTCCTGCCA AAAATCAAGC GGTGGCCCGG AGGCGGCGGC GTTCGTGAAG
  781 GTATCAATGG ACGGAGCACC GTACTTGAGG AAAATCGATT TGAGGATGTA TAAAGCTAGC
  841 ATGGTGAGCA AGGGCGAGGA GGATAACATG GCCATCATCA AAGGAGTTCA TGCGCTTCAA
  901 GGGTGCACAT GGGAGGGCTC CGTGAAACGG GCCACGAGTT CGAGATCGAG GGCGAGGGCG
  961 AGGGCCCGCC CCTACGAGGG CACCCAGACC CGCCCAAGCT GATGTGACCA AGGTGGCCCC
 1021 TTGCCTTCGC TGGGACATCC TGGTCCTTCA GGTCATGTAC CGCTCCAGGT CCTACGTTGA
1081 AGCACCCTGG TCTGACATCC CTCTG
//
Primer: reverse RDB16810_A8Lpb.seq
>D06157A6_A8LP_2_reverse_B03_06_ABI24.ab1
    1 ATTACAATTT ACCTCACTAA AGGGACAAAA GCTGGAGCTC CACCGCGGTG GCGGCCGCTC
   61 TAGAACTAGT GGATCCGGCG CCTGCACCAG CGCCCTTGTA CAGCTCGTCC ATGCCGCCGG
  121 TGGAGTGGCG GCCCTCGGCG CGTTCGTACT GTTCCACGAT GGTGTAGTCC TCGTTGTGGG
  181 AAAGGATGTC CAACTTGATG TCGACGTTGT AGGCGCCGGG CAGCTGCACG GGCTTCTTGG
  241 CCTTGTAGGT GGTCTTGACC TCAGCGTCGT AGTGGCCGCC GTCCTTCAGC TTCAGCCTCT
  301 GCTTGATCTC GCCCTTCAGG GCGCCGTCCT CGGGGTACAT CCGCTCAGTG GAGGCCTCCC
  361 AGCCCATGGT ACGACACTGC ATTACGGGGC CGTCGGAGGG GAAGTTGGTG CCGCGCAGCT
  421 TCACCTTGTA GATGAACTCG CCGTCCTGCA GGGAGGAGTC CTGGGTCACG GTCACCACGC
  481 CGCCGTCCTC GAAGTTCATC ACGCGCTCCC AATTGAAGCC CTCGGGGAAG GACAGCTTCA
  541 AGTAGTCGGG GATGTCGGCG GGGTGCTTCA CGTAGGCCTT GGAGCCGTAC ATGAACTGAG
  601 GGGACAGGAT GTCCCAGGCG AAGGGCAGGG GGCCACCCTT GGTCACCTTC AGCTTGGCGG
  661 TCTGGGTGCC CTCGTAGGGG CGGCCCTCGC CCTCGCCCTC GATCTCGAAC TCGTGGCCGT
  721 TCACGGAGCC CTCCATGTGC ACCTTGAAGC GCATGAACTC CTTGATGATG GCCATGTTAT
  781 CCTCCTCGCC CTTGCTCACC ATGCTAGCTT TATACATCCT CAAATCGATT TTCCTCAAGT
  841 ACGGTGCTCC GTCCATTGAT ACCTTCACGA ACGCCGCCGC CTCCGGGCCA CCGCTTGATT
  901 TTTGGCAGGA AACCATCACG TTCTTCCGGT ATGATCTCAC CGGTGGGCCA TCCCACAACT
  961 TGTGCCTTGG CCGGAGGTTT GGGCTGGGAT CTTTAGGACA AGCACTTCTT CTCTTTAGAT
 1021 CTAGTCCAGG ATCTCTCAAC GTCTCCAGCC TGCTTCAGCA AGCCTGCAGG TTAGTTAGCT
1081 TCCCGGCC
//
Primer: mCherry-check_R4 RDB16810_A8Lpc.seq
>D06157A6_A8LP_1_mCherry-check_R4_C03_09_ABI24.ab1
    1 CTGGGGCTTA GTAGGCTTGG AGCGTACATG AACTGAGGGG ACAGGATGTC CCAGGCGAAG
   61 GGCAGGGGGC CACCCTTGGT CACCTTCAGC TTGGCGGTCT GGGTGCCCTC GTAGGGGCGG
  121 CCCTCGCCCT CGCCCTCGAT CTCGAACTCG TGGCCGTTCA CGGAGCCCTC CATGTGCACC
  181 TTGAAGCGCA TGAACTCCTT GATGATGGCC ATGTTATCCT CCTCGCCCTT GCTCACCATG
  241 CTAGCTTTAT ACATCCTCAA ATCGATTTTC CTCAAGTACG GTGCTCCGTC CATTGATACC
  301 TTCACGAACG CCGCCGCCTC CGGGCCACCG CTTGATTTTT GGCAGGAAAC CATCACGTTC
  361 TTCCGGTATG ATCTCACCGG TGGCCATCCC ACAACTTGTG CCTTGGCCGG AGGTTTGGCT
  421 GGATCTTTAG GACAAGCACT CTTCTCCTTA GATCTAGGTC CAGGGTTCTC CTCCACGTCT
  481 CCAGCCTGCT TCAGCAGGCT GAAGTTAGTA GCTCCGCTTC CTGAGCCCTC CCACACATAA
  541 CCAGAGGGCA GCAATTCACG AATCCCAACT GCCGTCGGCT GTCCATCACT GTCCTTCACT
  601 ATGGCTTTGA TCCCAGGATG CAGATCGAGA AGCACCTGTC GGCACCGTCC GCAGGGGCTC
  661 AAGATGCCCC TGTTCTCATT TCCGATCGCG ACGATACAAG TCAGGTTGCC AGCTGCCGCA
  721 GCAGCAGCAG TGCCCAGCAC CACGAGTTCT GCACAAGGTC CCCCAGTAAA ATGATATACA
  781 TTGACACCAG TGAAGATGCG GCCGTCGCTA GAGAGAGCTG CGCTGGCGAC GCTGTAGTCT
  841 TCAGAGATGG GGATGCTGTT GATTGTAGCC GTTGCTCTTT CAATGAGGGT GGATTCTTCT
  901 TGAGACAAAA GGCTTGGCCC ATGGTGGCGA TATCAAGCTT ATCGATACCG TCGACCTCGA
  961 GGGGGGGGGC CCGGGTACCC AATTCGCCCT ATAGTGAGTC GTATTTACGC GCGCTTCACT
 1021 GGTCGTCGTT TTTACAACGT CGATGACCTG GGAAAACCCT GACGGTTAAC CAAACTTATT
1081 CGGCTTGGCC AGCACATCCC CCTTTTCGTC CAAGCTT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

2019.07.12

GNP_filter3_RDBDEP_html_190431.pl