Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK349 (BSD-P2A-mAID-mCherry2)

A plasmid for construction of N-terminal BSD-P2A-mAID-mCherry2 tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Please contact us to request the Exclusive MTA.
Remarks	This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。
備考	Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16810_A8Lpp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310) Region: T7 pro,MCS,insert 5'(BSD,P2A,mAID 5') Sequence file: RDB16810_A8Lpc.seq
>D06157A6_A8LP_2_pUC-M13_-100_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NGGNNNGCNN GGGTTTTCCN NNTCNCGACG TTGTAAAACG    61 ACGGCCAGTG NGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGGTAC CGGGCCCCCC   121 CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGCCACCA TGGCCAAGCC TTTGTCTCAA   181 GAAGAATCCA CCCTCATTGA AAGAGCAACG GCTACAATCA ACAGCATCCC CATCTCTGAA   241 GACTACAGCG TCGCCAGCGC AGCTCTCTCT AGCGACGGCC GCATCTTCAC TGGTGTCAAT   301 GTATATCATT TTACTGGGGG ACCTTGTGCA GAACTCGTGG TGCTGGGCAC TGCTGCTGCT   361 GCGGCAGCTG GCAACCTGAC TTGTATCGTC GCGATCGGAA ATGAGAACAG GGGCATCTTG   421 AGCCCCTGCG GACGGTGCCG ACAGGTGCTT CTCGATCTGC ATCCTGGGAT CAAAGCCATA   481 GTGAAGGACA GTGATGGACA GCCGACGGCA GTTGGGATTC GTGAATTGCT GCCCTCTGGT   541 TATGTGTGGG AGGGCTCAGG AAGCGGAGCT ACTAACTTCA GCCTGCTGAA GCAGGCTGGA   601 GACGTGGAGG AGAACCCTGG ACCTAGATCT AAGGAGAAGA GTGCTTGTCC TAAAGATCCA   661 GCCAAACCTC CGGCCAAGGC ACAAGTTGTG GGATGGCCAC CGGTGAGATC ATACCGGAAG   721 AACGTGATGG TTTCCTGCCN AAAATCAAGC GGTGGNCCGG ANGCGGCGGC GTTCGTGAAN   781 GTATCANTGG ACGGNNCACC GTACTTGANG AAAATCGNTT TGANGATGTA TAAAGCTAGC   841 ATNGTGAGCA AGGGCGANGN NGNNNACATG GNNNTCATNN ANNNANTTNA TGCNNTTNNA   901 NGNNGCNNNN NGNNNGGNNN CNNGNANNNG NNNNNNANTT NNANNNNNNN GNNNANGNNN 961 NNGNNNNNNN NNN //
Primer: reverse (Pr0099) Region: MCS,insert 3'(liker,mCherry2 3') Sequence file: RDB16810_A8Lpd.seq
>D06157A6_A8LP_2_reverse_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNAA AGGGANNNAA GCTGGAGCTC CACCGCGGTG GCGGCCGCTC    61 TAGAACTAGT GGATCCGGCG CCTGCACCAG CGCCCTTGTA CAGCTCGTCC ATGCCGCCGG   121 TGGAGTGGCG GCCCTCGGCG CGTTCGTACT GTTCCACGAT GGTGTAGTCC TCGTTGTGGG   181 AAAGGATGTC CAACTTGATG TCGACGTTGT AGGCGCCGGG CAGCTGCACG GGCTTCTTGG   241 CCTTGTAGGT GGTCTTGACC TCAGCGTCGT AGTGGCCGCC GTCCTTCAGC TTCAGCCTCT   301 GCTTGATCTC GCCCTTCAGG GCGCCGTCCT CGGGGTACAT CCGCTCAGTG GAGGCCTCCC   361 AGCCCATGGT ACGACACTGC ATTACGGGGC CGTCGGAGGG GAAGTTGGTG CCGCGCAGCT   421 TCACCTTGTA GATGAACTCG CCGTCCTGCA GGGAGGAGTC CTGGGTCACG GTCACCACGC   481 CGCCGTCCTC GAAGTTCATC ACGCGCTCCC AATTGAAGCC CTCGGGGAAG GACAGCTTCA   541 AGTAGTCGGG GATGTCGGCG GGGTGCTTCA CGTAGGCCTT GGAGCCGTAC ATGAACTGAG   601 GGGACAGGAT GTCCCAGGCG AAGGGCAGGG GGCCACCCTT GGTCACCTTC AGCTTGGCGG   661 TCTGGGTGCC CTCGTANGGG CGGCCCTCGC CCTCGCCCTC GATCTCGAAC TCGTGGCCGT   721 TCACGGANCC CTCCATGTGC ACCTNGAAGC GCATGAACTC CTTGATGANG GCCATGTTAT   781 CCTCCTCGCC CTTGCTCACC ATGCTAGCTT TATACATCCT CAAATCGATT TTCCTCNAGT   841 ACGGNGCTCC NNCNATTGNT ACCTTCNCGA ACGCCGCCNC CTCNNGGCCA CCGCTNNANT 901 TTTGNNNNNA ANNNNTCACN NNCNNNNGGT ATGNNNNCNN NNNNNGNNNN NNNNNN //
Primer: mCherry-check_R4 (Pr0527) Region: insert mid(mCherry2 5',mAID,P2A,BSD 3') Sequence file: RDB16810_A8Lpe.seq
>D06157A6_A8LP_2_mCherry-check_R4_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG NNNNTNNNTG NACTGNNGGG NNAGGNNGTC CCAGGCGAAG    61 GGCAGGGGGC CACCCTTGGT CACCTTCAGC TTGGCGGTCT GGGTGCCCTC GTAGGGGCGG   121 CCCTCGCCCT CGCCCTCGAT CTCGAACTCG TGGCCGTTCA CGGAGCCCTC CATGTGCACC   181 TTGAAGCGCA TGAACTCCTT GATGATGGCC ATGTTATCCT CCTCGCCCTT GCTCACCATG   241 CTAGCTTTAT ACATCCTCAA ATCGATTTTC CTCAAGTACG GTGCTCCGTC CATTGATACC   301 TTCACGAACG CCGCCGCCTC CGGGCCACCG CTTGATTTTT GGCAGGAAAC CATCACGTTC   361 TTCCGGTATG ATCTCACCGG TGGCCATCCC ACAACTTGTG CCTTGGCCGG AGGTTTGGCT   421 GGATCTTTAG GACAAGCACT CTTCTCCTTA GATCTAGGTC CAGGGTTCTC CTCCACGTCT   481 CCAGCCTGCT TCAGCAGGCT GAAGTTAGTA GCTCCGCTTC CTGAGCCCTC CCACACATAA   541 CCAGAGGGCA GCAATTCACG AATCCCAACT GCCGTCGGCT GTCCATCACT GTCCTTCACT   601 ATGGCTTTGA TCCCAGGATG CAGATCGAGA AGCACCTGTC GGCACCGTCC GCAGGGGCTC   661 AAGATGCCCC TGTTCTCATT TCCGATCGCG ACGATACAAG TCAGGTTGCC AGCTGCCGCA   721 GCAGCAGCAG TGCCCAGCAC CACGAGTTCT GCACAANGTC CCCCAGTAAA ATGATATACA   781 TTGACACCAG TGAAGANGCG GCCGTCGCTA GAGAGAGCTG CGCTGGCGAC GCTGTANNCT   841 TCAGANATGG GGANGCTGTT GANTGTAGCC GTNGCTCTTT CNANGAGGGN GGANTCTTCT   901 TGANANNAAN NNCTNNNNCN NNNGNGGNNA NATCNNNNNT ATCGATACCG NCNANNNNNN 961 NGGGGGGGNN CNNGNNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.02.27

GNP_filter3_RDBDEP_html_230227.pl