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pMK346 (Hygro-P2A-mAID-mCherry2)

A plasmid for construction of N-terminal Hygro-P2A-mAID-mCherry2 tagging donors.

Catalog number RDB16807
Resource name pMK346 (Hygro-P2A-mAID-mCherry2)
Clone info. A plasmid for construction of N-terminal Hygro-P2A-mAID-mCherry2 tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Discosoma sp. RFP cDNA
Depositor Kanemaki, Masato |
 
Other clones in our bank Arabidopsis thaliana AXR3 (KEGG orthology K14484) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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Remarks
The BIOLOGICAL RESOURCE may not be used for COMMERCIAL PURPOSES. Please read the Exclusive MTA (Clontech FPs).
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RDB16807 pMK346 (Hygro-P2A-mAID-mCherry2) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16807_A8Lpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 RDB16807_A8Lpa.seq
>D06157A3_A8LP_2_pUC-M13_-100_F01_16_ABI24.ab1
    1 GGCCGTCAGC GGATTAGTTG GGTACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA
   61 CGGCCAGTGA GCGCGCGTAA TACGACTCAC TATAGGGCGA ATTGGGTACC GGGCCCCCCC
  121 TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGCCACCAT GAAAAAGCCT GAACTCACCG
  181 CGACGTCTGT CGAGAAGTTT CTGATCGAAA AGTTCGACAG CGTCTCCGAC CTGATGCAGC
  241 TCTCGGAGGG CGAAGAATCT CGTGCTTTCA GCTTCGATGT AGGAGGGCGT GGATATGTCC
  301 TGCGGGTAAA TAGCTGCGCC GATGGTTTCT ACAAAGATCG TTATGTTTAT CGGCACTTTG
  361 CATCGGCCGC GCTCCCGATT CCGGAAGTGC TTGACATTGG GGAATTCAGC GAGAGCCTGA
  421 CCTATTGCAT CTCCCGCCGT GCACAGGGTG TCACGTTGCA AGACCTGCCT GAAACCGAAC
  481 TGCCCGCTGT TCTGCAGCCG GTCGCGGAGG CCATGGATGC GATCGCTGCG GCCGATCTTA
  541 GCCAGACGAG CGGGTTCGGC CCATTCGGAC CGCAAGGAAT CGGTCAATAC ACTACATGGC
  601 GTGATTTCAT ATGCGCGATT GCTGATCCCC ATGTGTATCA CTGGCAAACT GTGATGGACG
  661 ACACCGTCAG TGCGTCCGTC GCGCAGGCTC TCGATGAGCT GATGCTTTGG GCCGAGGACT
  721 GCCCCGAAGT CCGGCACCTC GTGCACGCGG ATTTCGGCTC CAACAATGTC CTGACGGACA
  781 ATGGCCGCAT AACAGCGGTC ATTGACTGGA GCGAGGCGAT GTTCGGGGAT TCCCAATACG
  841 AGGTCGCCAA CATCTTCTTC TGGAGGCCGT GGTTGGCTTG TATGGAGCAG CAGACGCGCT
  901 ACTTCGAGCG GAGGCATCCG GAGCTTGCAG GATCGCCGCG GCTCCGGGCG TATATGCTCC
  961 CGCATTGGGT CTTGACCAAA CTCTATCAGA GCTTTGCTTG ACGGCAATTT CGATGATGCA
 1021 GCTTTGAGCG CAGGTTCGGA TGCGAACGCA TCGTCCGAAT CCAGAGCTGG AACCTGCTCC
1081 GGGCCGTTAC AACAAAATTC GATCTG
//
Primer: reverse RDB16807_A8Lpb.seq
>D06157A3_A8LP_2_reverse_G01_19_ABI24.ab1
    1 CTTGAAATTT AAACCTCACT AAAAGGGAAC AAAAGCTGGA GCTCCACCGC GGTGGCGGCC
   61 GCTCTAGAAC TAGTGGATCC GGCGCCTGCA CCAGCGCCCT TGTACAGCTC GTCCATGCCG
  121 CCGGTGGAGT GGCGGCCCTC GGCGCGTTCG TACTGTTCCA CGATGGTGTA GTCCTCGTTG
  181 TGGGAAAGGA TGTCCAACTT GATGTCGACG TTGTAGGCGC CGGGCAGCTG CACGGGCTTC
  241 TTGGCCTTGT AGGTGGTCTT GACCTCAGCG TCGTAGTGGC CGCCGTCCTT CAGCTTCAGC
  301 CTCTGCTTGA TCTCGCCCTT CAGGGCGCCG TCCTCGGGGT ACATCCGCTC AGTGGAGGCC
  361 TCCCAGCCCA TGGTACGACA CTGCATTACG GGGCCGTCGG AGGGGAAGTT GGTGCCGCGC
  421 AGCTTCACCT TGTAGATGAA CTCGCCGTCC TGCAGGGAGG AGTCCTGGGT CACGGTCACC
  481 ACGCCGCCGT CCTCGAAGTT CATCACGCGC TCCCAATTGA AGCCCTCGGG GAAGGACAGC
  541 TTCAAGTAGT CGGGGATGTC GGCGGGGTGC TTCACGTAGG CCTTGGAGCC GTACATGAAC
  601 TGAGGGGACA GGATGTCCCA GGCGAAGGGC AGGGGGCCAC CCTTGGTCAC CTTCAGCTTG
  661 GCGGTCTGGG TGCCCTCGTA GGGGCGGCCC TCGCCCTCGC CCTCGATCTC GAACTCGTGG
  721 CCGTTCACGG AGCCCTCCAT GTGCACCTTG AAGCGCATGA ACTCCTTGAT GATGGCCATG
  781 TTATCCTCCT CGCCCTTGCT CACCATGCTA GCTTTATACA TCCTCAAATC GATTTTCCTC
  841 AAGTACGGTG CTCCGTCCAT TGATACCTTC ACGAACGCCG CCGCCTCCGG GCCACCGCTT
  901 GATTTTTGGC AGGAAACCAT CACGTTCTTC CGGTATGATC TCACCGGTGG CCCATCCCAC
  961 AACTTGTGCC CTTGGCCCGG AGGTTTGGGC TGGATCTTTA GACAAGGCAC TCTTCTGTTA
 1021 GATCTAGTCC AGGATCTCCT CAACGTCTCC AGGCCTGCTC CAGCCAAGCC TGAAAGGTTA
1081 GGTTAGGCAT CCCCGACCTA
//
Primer: HygR-C_F RDB16807_A8Lpc.seq
>D06157A3_A8LP_2_HygR-C_F_H01_22_ABI24.ab1
    1 GGGAATTCGG GGGATCCAAT ACGAGGTCGC CACATCTTCT TCTGGAGGCC GTGGTTGGCT
   61 TGTATGGAGC AGCAGACGCG CTACTTCGAG CGGAGGCATC CGGAGCTTGC AGGATCGCCG
  121 CGGCTCCGGG CGTATATGCT CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC
  181 GGCAATTTCG ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA
  241 GCCGGGACTG TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG GACCGATGGC
  301 TGTGTAGAAG TACTCGCCGA TAGTGGAAAC CGACGCCCCA GCACTCGTCC GAGGGCAAAG
  361 GAATCAGGAA GCGGAGCTAC TAACTTCAGC CTGCTGAAGC AGGCTGGAGA CGTGGAGGAG
  421 AACCCTGGAC CTAGATCTAA GGAGAAGAGT GCTTGTCCTA AAGATCCAGC CAAACCTCCG
  481 GCCAAGGCAC AAGTTGTGGG ATGGCCACCG GTGAGATCAT ACCGGAAGAA CGTGATGGTT
  541 TCCTGCCAAA AATCAAGCGG TGGCCCGGAG GCGGCGGCGT TCGTGAAGGT ATCAATGGAC
  601 GGAGCACCGT ACTTGAGGAA AATCGATTTG AGGATGTATA AAGCTAGCAT GGTGAGCAAG
  661 GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAG
  721 GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG
  781 GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCCTGCCCT TCGCCTGGGA
  841 CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT
  901 CCCCGACTAC TTGAAAGCTG TCCTTCCCCG AGGCTTCAAT TGGGAGCGCG TGATGAACTT
  961 CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACC GCGAGTTCAT
 1021 CTACAGGTGA AGCTGCGCGG CACCAACTCC CCACGACGGA CCTCCGTAAT GCAGTGATCT
1081 GTAATCCTCA AT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

2019.07.12

GNP_filter3_RDBDEP_html_190431.pl