Sequencing and PCR primers for primer information.
A plasmid for construction of N-terminal Hygro-P2A-mAID-mCherry2 tagging donors.
| Catalog number | RDB16807 |
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| Resource name | pMK346 (Hygro-P2A-mAID-mCherry2) |
| Clone info. | A plasmid for construction of N-terminal Hygro-P2A-mAID-mCherry2 tagging donors. |
| Vector backbone | pBluescript (plasmid) |
| Size of vector backbone | 2.9 kb |
| Selectable markers | Am^r (E. coli) |
| Gene/insert name | Arabidopsis thaliana AtIAA17 cDNA Discosoma sp. RFP cDNA |
| Depositor | Kanemaki, Masato | |
| Other clones in our bank | Arabidopsis thaliana AXR3 (KEGG orthology K14484) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Exclusive MTA [Link] |
| Remarks | The BIOLOGICAL RESOURCE may not be used for COMMERCIAL PURPOSES. Please read the Exclusive MTA (Clontech FPs). |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB16807 | pMK346 (Hygro-P2A-mAID-mCherry2) | DNA solution |
Electronic file
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Auxin Inducible Degron (AID) System (English text) |
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| Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB16807_A8Lpp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pUC-M13_-100 | RDB16807_A8Lpa.seq |
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>D06157A3_A8LP_2_pUC-M13_-100_F01_16_ABI24.ab1 1 GGCCGTCAGC GGATTAGTTG GGTACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA 61 CGGCCAGTGA GCGCGCGTAA TACGACTCAC TATAGGGCGA ATTGGGTACC GGGCCCCCCC 121 TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGCCACCAT GAAAAAGCCT GAACTCACCG 181 CGACGTCTGT CGAGAAGTTT CTGATCGAAA AGTTCGACAG CGTCTCCGAC CTGATGCAGC 241 TCTCGGAGGG CGAAGAATCT CGTGCTTTCA GCTTCGATGT AGGAGGGCGT GGATATGTCC 301 TGCGGGTAAA TAGCTGCGCC GATGGTTTCT ACAAAGATCG TTATGTTTAT CGGCACTTTG 361 CATCGGCCGC GCTCCCGATT CCGGAAGTGC TTGACATTGG GGAATTCAGC GAGAGCCTGA 421 CCTATTGCAT CTCCCGCCGT GCACAGGGTG TCACGTTGCA AGACCTGCCT GAAACCGAAC 481 TGCCCGCTGT TCTGCAGCCG GTCGCGGAGG CCATGGATGC GATCGCTGCG GCCGATCTTA 541 GCCAGACGAG CGGGTTCGGC CCATTCGGAC CGCAAGGAAT CGGTCAATAC ACTACATGGC 601 GTGATTTCAT ATGCGCGATT GCTGATCCCC ATGTGTATCA CTGGCAAACT GTGATGGACG 661 ACACCGTCAG TGCGTCCGTC GCGCAGGCTC TCGATGAGCT GATGCTTTGG GCCGAGGACT 721 GCCCCGAAGT CCGGCACCTC GTGCACGCGG ATTTCGGCTC CAACAATGTC CTGACGGACA 781 ATGGCCGCAT AACAGCGGTC ATTGACTGGA GCGAGGCGAT GTTCGGGGAT TCCCAATACG 841 AGGTCGCCAA CATCTTCTTC TGGAGGCCGT GGTTGGCTTG TATGGAGCAG CAGACGCGCT 901 ACTTCGAGCG GAGGCATCCG GAGCTTGCAG GATCGCCGCG GCTCCGGGCG TATATGCTCC 961 CGCATTGGGT CTTGACCAAA CTCTATCAGA GCTTTGCTTG ACGGCAATTT CGATGATGCA 1021 GCTTTGAGCG CAGGTTCGGA TGCGAACGCA TCGTCCGAAT CCAGAGCTGG AACCTGCTCC 1081 GGGCCGTTAC AACAAAATTC GATCTG // |
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| Primer: reverse | RDB16807_A8Lpb.seq |
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>D06157A3_A8LP_2_reverse_G01_19_ABI24.ab1 1 CTTGAAATTT AAACCTCACT AAAAGGGAAC AAAAGCTGGA GCTCCACCGC GGTGGCGGCC 61 GCTCTAGAAC TAGTGGATCC GGCGCCTGCA CCAGCGCCCT TGTACAGCTC GTCCATGCCG 121 CCGGTGGAGT GGCGGCCCTC GGCGCGTTCG TACTGTTCCA CGATGGTGTA GTCCTCGTTG 181 TGGGAAAGGA TGTCCAACTT GATGTCGACG TTGTAGGCGC CGGGCAGCTG CACGGGCTTC 241 TTGGCCTTGT AGGTGGTCTT GACCTCAGCG TCGTAGTGGC CGCCGTCCTT CAGCTTCAGC 301 CTCTGCTTGA TCTCGCCCTT CAGGGCGCCG TCCTCGGGGT ACATCCGCTC AGTGGAGGCC 361 TCCCAGCCCA TGGTACGACA CTGCATTACG GGGCCGTCGG AGGGGAAGTT GGTGCCGCGC 421 AGCTTCACCT TGTAGATGAA CTCGCCGTCC TGCAGGGAGG AGTCCTGGGT CACGGTCACC 481 ACGCCGCCGT CCTCGAAGTT CATCACGCGC TCCCAATTGA AGCCCTCGGG GAAGGACAGC 541 TTCAAGTAGT CGGGGATGTC GGCGGGGTGC TTCACGTAGG CCTTGGAGCC GTACATGAAC 601 TGAGGGGACA GGATGTCCCA GGCGAAGGGC AGGGGGCCAC CCTTGGTCAC CTTCAGCTTG 661 GCGGTCTGGG TGCCCTCGTA GGGGCGGCCC TCGCCCTCGC CCTCGATCTC GAACTCGTGG 721 CCGTTCACGG AGCCCTCCAT GTGCACCTTG AAGCGCATGA ACTCCTTGAT GATGGCCATG 781 TTATCCTCCT CGCCCTTGCT CACCATGCTA GCTTTATACA TCCTCAAATC GATTTTCCTC 841 AAGTACGGTG CTCCGTCCAT TGATACCTTC ACGAACGCCG CCGCCTCCGG GCCACCGCTT 901 GATTTTTGGC AGGAAACCAT CACGTTCTTC CGGTATGATC TCACCGGTGG CCCATCCCAC 961 AACTTGTGCC CTTGGCCCGG AGGTTTGGGC TGGATCTTTA GACAAGGCAC TCTTCTGTTA 1021 GATCTAGTCC AGGATCTCCT CAACGTCTCC AGGCCTGCTC CAGCCAAGCC TGAAAGGTTA 1081 GGTTAGGCAT CCCCGACCTA // |
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| Primer: HygR-C_F | RDB16807_A8Lpc.seq |
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>D06157A3_A8LP_2_HygR-C_F_H01_22_ABI24.ab1 1 GGGAATTCGG GGGATCCAAT ACGAGGTCGC CACATCTTCT TCTGGAGGCC GTGGTTGGCT 61 TGTATGGAGC AGCAGACGCG CTACTTCGAG CGGAGGCATC CGGAGCTTGC AGGATCGCCG 121 CGGCTCCGGG CGTATATGCT CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC 181 GGCAATTTCG ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA 241 GCCGGGACTG TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG GACCGATGGC 301 TGTGTAGAAG TACTCGCCGA TAGTGGAAAC CGACGCCCCA GCACTCGTCC GAGGGCAAAG 361 GAATCAGGAA GCGGAGCTAC TAACTTCAGC CTGCTGAAGC AGGCTGGAGA CGTGGAGGAG 421 AACCCTGGAC CTAGATCTAA GGAGAAGAGT GCTTGTCCTA AAGATCCAGC CAAACCTCCG 481 GCCAAGGCAC AAGTTGTGGG ATGGCCACCG GTGAGATCAT ACCGGAAGAA CGTGATGGTT 541 TCCTGCCAAA AATCAAGCGG TGGCCCGGAG GCGGCGGCGT TCGTGAAGGT ATCAATGGAC 601 GGAGCACCGT ACTTGAGGAA AATCGATTTG AGGATGTATA AAGCTAGCAT GGTGAGCAAG 661 GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAG 721 GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG 781 GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCCTGCCCT TCGCCTGGGA 841 CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT 901 CCCCGACTAC TTGAAAGCTG TCCTTCCCCG AGGCTTCAAT TGGGAGCGCG TGATGAACTT 961 CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACC GCGAGTTCAT 1021 CTACAGGTGA AGCTGCGCGG CACCAACTCC CCACGACGGA CCTCCGTAAT GCAGTGATCT 1081 GTAATCCTCA AT // |
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Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
2019.07.12
GNP_filter3_RDBDEP_html_190431.pl