Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK346 (Hygro-P2A-mAID-mCherry2)

A plasmid for construction of N-terminal Hygro-P2A-mAID-mCherry2 tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose)[Link]
Remarks	The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose).

Distribution information (domestic users) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 専用MTA (Takara Bio USA, Inc. FPs, 英語版のみ、非営利機関による非営利学術研究用)[Link]
備考	本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。専用MTA（Takara Bio USA, Inc. FPs, 英語版のみ、非営利機関による非営利学術研究用）をご参照ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16807_A8Lpp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 Region: T7 pro, insert 5' (HygR 5') Sequence file: RDB16807_A8Lpa.seq
>D06157A3_A8LP_2_pUC-M13_-100_F01_16_ABI24.ab1     1 GGCCGTCAGC GGATTAGTTG GGTACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA    61 CGGCCAGTGA GCGCGCGTAA TACGACTCAC TATAGGGCGA ATTGGGTACC GGGCCCCCCC   121 TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGCCACCAT GAAAAAGCCT GAACTCACCG   181 CGACGTCTGT CGAGAAGTTT CTGATCGAAA AGTTCGACAG CGTCTCCGAC CTGATGCAGC   241 TCTCGGAGGG CGAAGAATCT CGTGCTTTCA GCTTCGATGT AGGAGGGCGT GGATATGTCC   301 TGCGGGTAAA TAGCTGCGCC GATGGTTTCT ACAAAGATCG TTATGTTTAT CGGCACTTTG   361 CATCGGCCGC GCTCCCGATT CCGGAAGTGC TTGACATTGG GGAATTCAGC GAGAGCCTGA   421 CCTATTGCAT CTCCCGCCGT GCACAGGGTG TCACGTTGCA AGACCTGCCT GAAACCGAAC   481 TGCCCGCTGT TCTGCAGCCG GTCGCGGAGG CCATGGATGC GATCGCTGCG GCCGATCTTA   541 GCCAGACGAG CGGGTTCGGC CCATTCGGAC CGCAAGGAAT CGGTCAATAC ACTACATGGC   601 GTGATTTCAT ATGCGCGATT GCTGATCCCC ATGTGTATCA CTGGCAAACT GTGATGGACG   661 ACACCGTCAG TGCGTCCGTC GCGCAGGCTC TCGATGAGCT GATGCTTTGG GCCGAGGACT   721 GCCCCGAAGT CCGGCACCTC GTGCACGCGG ATTTCGGCTC CAACAATGTC CTGACGGACA   781 ATGGCCGCAT AACAGCGGTC ATTGACTGGA GCGAGGCGAT GTTCGGGGAT TCCCAATACG   841 AGGTCGCCAA CATCTTCTTC TGGAGGCCGT GGTTGGCTTG TATGGAGCAG CAGACGCGCT   901 ACTTCGAGCG GAGGCATCCG GAGCTTGCAG GATCGCCGCG GCTCCGGGCG TATATGCTCC   961 CGCATTGGGT CTTGACCAAA CTCTATCAGA GCTTTGCTTG ACGGCAATTT CGATGATGCA  1021 GCTTTGAGCG CAGGTTCGGA TGCGAACGCA TCGTCCGAAT CCAGAGCTGG AACCTGCTCC 1081 GGGCCGTTAC AACAAAATTC GATCTG //
Primer: reverse Region: T3 pro, insert 5' (Linker, mCherry2, mAID) Sequence file: RDB16807_A8Lpb.seq
>D06157A3_A8LP_2_reverse_G01_19_ABI24.ab1     1 CTTGAAATTT AAACCTCACT AAAAGGGAAC AAAAGCTGGA GCTCCACCGC GGTGGCGGCC    61 GCTCTAGAAC TAGTGGATCC GGCGCCTGCA CCAGCGCCCT TGTACAGCTC GTCCATGCCG   121 CCGGTGGAGT GGCGGCCCTC GGCGCGTTCG TACTGTTCCA CGATGGTGTA GTCCTCGTTG   181 TGGGAAAGGA TGTCCAACTT GATGTCGACG TTGTAGGCGC CGGGCAGCTG CACGGGCTTC   241 TTGGCCTTGT AGGTGGTCTT GACCTCAGCG TCGTAGTGGC CGCCGTCCTT CAGCTTCAGC   301 CTCTGCTTGA TCTCGCCCTT CAGGGCGCCG TCCTCGGGGT ACATCCGCTC AGTGGAGGCC   361 TCCCAGCCCA TGGTACGACA CTGCATTACG GGGCCGTCGG AGGGGAAGTT GGTGCCGCGC   421 AGCTTCACCT TGTAGATGAA CTCGCCGTCC TGCAGGGAGG AGTCCTGGGT CACGGTCACC   481 ACGCCGCCGT CCTCGAAGTT CATCACGCGC TCCCAATTGA AGCCCTCGGG GAAGGACAGC   541 TTCAAGTAGT CGGGGATGTC GGCGGGGTGC TTCACGTAGG CCTTGGAGCC GTACATGAAC   601 TGAGGGGACA GGATGTCCCA GGCGAAGGGC AGGGGGCCAC CCTTGGTCAC CTTCAGCTTG   661 GCGGTCTGGG TGCCCTCGTA GGGGCGGCCC TCGCCCTCGC CCTCGATCTC GAACTCGTGG   721 CCGTTCACGG AGCCCTCCAT GTGCACCTTG AAGCGCATGA ACTCCTTGAT GATGGCCATG   781 TTATCCTCCT CGCCCTTGCT CACCATGCTA GCTTTATACA TCCTCAAATC GATTTTCCTC   841 AAGTACGGTG CTCCGTCCAT TGATACCTTC ACGAACGCCG CCGCCTCCGG GCCACCGCTT   901 GATTTTTGGC AGGAAACCAT CACGTTCTTC CGGTATGATC TCACCGGTGG CCCATCCCAC   961 AACTTGTGCC CTTGGCCCGG AGGTTTGGGC TGGATCTTTA GACAAGGCAC TCTTCTGTTA  1021 GATCTAGTCC AGGATCTCCT CAACGTCTCC AGGCCTGCTC CAGCCAAGCC TGAAAGGTTA 1081 GGTTAGGCAT CCCCGACCTA //
Primer: HygR-C_F Region: insert mid (HygR 3', P2A, mAID, mCherry) Sequence file: RDB16807_A8Lpc.seq
>D06157A3_A8LP_2_HygR-C_F_H01_22_ABI24.ab1     1 GGGAATTCGG GGGATCCAAT ACGAGGTCGC CACATCTTCT TCTGGAGGCC GTGGTTGGCT    61 TGTATGGAGC AGCAGACGCG CTACTTCGAG CGGAGGCATC CGGAGCTTGC AGGATCGCCG   121 CGGCTCCGGG CGTATATGCT CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC   181 GGCAATTTCG ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA   241 GCCGGGACTG TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG GACCGATGGC   301 TGTGTAGAAG TACTCGCCGA TAGTGGAAAC CGACGCCCCA GCACTCGTCC GAGGGCAAAG   361 GAATCAGGAA GCGGAGCTAC TAACTTCAGC CTGCTGAAGC AGGCTGGAGA CGTGGAGGAG   421 AACCCTGGAC CTAGATCTAA GGAGAAGAGT GCTTGTCCTA AAGATCCAGC CAAACCTCCG   481 GCCAAGGCAC AAGTTGTGGG ATGGCCACCG GTGAGATCAT ACCGGAAGAA CGTGATGGTT   541 TCCTGCCAAA AATCAAGCGG TGGCCCGGAG GCGGCGGCGT TCGTGAAGGT ATCAATGGAC   601 GGAGCACCGT ACTTGAGGAA AATCGATTTG AGGATGTATA AAGCTAGCAT GGTGAGCAAG   661 GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAG   721 GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG   781 GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCCTGCCCT TCGCCTGGGA   841 CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT   901 CCCCGACTAC TTGAAAGCTG TCCTTCCCCG AGGCTTCAAT TGGGAGCGCG TGATGAACTT   961 CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACC GCGAGTTCAT  1021 CTACAGGTGA AGCTGCGCGG CACCAACTCC CCACGACGGA CCTCCGTAAT GCAGTGATCT 1081 GTAATCCTCA AT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2021.11.02

GNP_filter3_RDBDEP_html_211029.pl