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pMK346 (Hygro-P2A-mAID-mCherry2) (#RDB16807)

A plasmid for construction of N-terminal Hygro-P2A-mAID-mCherry2 tagging donors.

Clone info. A plasmid for construction of N-terminal Hygro-P2A-mAID-mCherry2 tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Discosoma sp. RFP cDNA
Depositor|Developer Kanemaki, Masato |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。

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RDB16807 pMK346 (Hygro-P2A-mAID-mCherry2) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMK346 (Hygro-P2A-mAID-mCherry2) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16807).

Reference section:

Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16807_A8Lpp1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310)
Region: T7 pro, insert 5'(HygR 5')
Sequence file: RDB16807_A8Lpd.seq check
>D06157A3_A8LP_2_pUC-M13_-100_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNTG GGNANGCCNN GNNTTTCCCN NTCNCGACGT TGTAAAACGA
   61 CGGCCAGTGA GCGCGCGTAA TACGACTCAC TATAGGGCGA ATTGGGTACC GGGCCCCCCC
  121 TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGCCACCAT GAAAAAGCCT GAACTCACCG
  181 CGACGTCTGT CGAGAAGTTT CTGATCGAAA AGTTCGACAG CGTCTCCGAC CTGATGCAGC
  241 TCTCGGAGGG CGAAGAATCT CGTGCTTTCA GCTTCGATGT AGGAGGGCGT GGATATGTCC
  301 TGCGGGTAAA TAGCTGCGCC GATGGTTTCT ACAAAGATCG TTATGTTTAT CGGCACTTTG
  361 CATCGGCCGC GCTCCCGATT CCGGAAGTGC TTGACATTGG GGAATTCAGC GAGAGCCTGA
  421 CCTATTGCAT CTCCCGCCGT GCACAGGGTG TCACGTTGCA AGACCTGCCT GAAACCGAAC
  481 TGCCCGCTGT TCTGCAGCCG GTCGCGGAGG CCATGGATGC GATCGCTGCG GCCGATCTTA
  541 GCCAGACGAG CGGGTTCGGC CCATTCGGAC CGCAAGGAAT CGGTCAATAC ACTACATGGC
  601 GTGATTTCAT ATGCGCGATT GCTGATCCCC ATGTGTATCA CTGGCAAACT GTGATGGACG
  661 ACACCGTCAG TGCGTCCGTC GCGCANGCTC TCGATGAGCT GATGCTTTGG GCCGANGACT
  721 GCCCCGAAGT CCGGCACCTC GTGCACGCGG ATTTCNGCTC CAACAATGTC CTGACNGACA
  781 ATGGCCGCAT AACAGCGGTC ATTGACTGGA NCGAGGCGAT GTTCGGGGNN TCCCNATACN
  841 ANGTCGCNNA CNTCTTCTTC TGGANGNNGN NNTTGGNTTG TATNGANCAN CANANNCNNT
  901 ACTTCNNNNN NNNGNNTCNN NNNCNNGNNN NNNCNNNNNN GNNNNNGNNN NANNNNNNNC
961 NNNNNNNGNN NNNNNNNN
//
Primer: reverse (Pr0099)
Region: insert 3'(Linker,mCherry2 3')
Sequence file: RDB16807_A8Lpe.seq check
>D06157A3_A8LP_2_reverse_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGGNNN NNAAGCTGGA GCTCCNCCGC GGTGGCGGCC
   61 GCTCTAGAAC TAGTGGATCC GGCGCCTGCA CCAGCGCCCT TGTACAGCTC GTCCATGCCG
  121 CCGGTGGAGT GGCGGCCCTC GGCGCGTTCG TACTGTTCCA CGATGGTGTA GTCCTCGTTG
  181 TGGGAAAGGA TGTCCAACTT GATGTCGACG TTGTAGGCGC CGGGCAGCTG CACGGGCTTC
  241 TTGGCCTTGT AGGTGGTCTT GACCTCAGCG TCGTAGTGGC CGCCGTCCTT CAGCTTCAGC
  301 CTCTGCTTGA TCTCGCCCTT CAGGGCGCCG TCCTCGGGGT ACATCCGCTC AGTGGAGGCC
  361 TCCCAGCCCA TGGTACGACA CTGCATTACG GGGCCGTCGG AGGGGAAGTT GGTGCCGCGC
  421 AGCTTCACCT TGTAGATGAA CTCGCCGTCC TGCAGGGAGG AGTCCTGGGT CACGGTCACC
  481 ACGCCGCCGT CCTCGAAGTT CATCACGCGC TCCCAATTGA AGCCCTCGGG GAAGGACAGC
  541 TTCAAGTAGT CGGGGATGTC GGCGGGGTGC TTCACGTAGG CCTTGGAGCC GTACATGAAC
  601 TGAGGGGACA GGATGTCCCA GGCGAAGGGC AGGGGGCCAC CCTTGGTCAC CTTCAGCTTG
  661 GCGGTCTGGG TGCCCTCGTA GGGGCGGCCC TCGCCCTCGC CCTCGATCTC GAACTCGTGG
  721 CCGTTCACGG ANCCCTCCAT GTGCACCTTG AAGCGCATGA ACTCCTTGAT GANGGCCATG
  781 TTATCCTCCT CGCCCTTGCT CACCATGCTA NCTTTATACA TCCTCAAATC NATTTNCCTC
  841 ANGTACGGNG CTNCNNCCNT TGATACCTTC ACGAACGCCG CNNCCNCCNG GCCACCNCTN
901 NNNTTTTNNN ANGAAANCAT NNCNNNCNNN CGGNATGATC NNNNNNNNNN
//
Primer: HygR-C_F (Pr0501)
Region: insert mid (HygR 3',P2A,mAID,mCherry2 5')
Sequence file: RDB16807_A8Lpf.seq check
>D06157A3_A8LP_2_HygR-C_F_H01_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGANGTCGC NNCATCTTCT TCTGGAGGCC GTGGTTGGCT
   61 TGTATGGAGC AGCAGACGCG CTACTTCGAG CGGAGGCATC CGGAGCTTGC AGGATCGCCG
  121 CGGCTCCGGG CGTATATGCT CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC
  181 GGCAATTTCG ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA
  241 GCCGGGACTG TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG GACCGATGGC
  301 TGTGTAGAAG TACTCGCCGA TAGTGGAAAC CGACGCCCCA GCACTCGTCC GAGGGCAAAG
  361 GAATCAGGAA GCGGAGCTAC TAACTTCAGC CTGCTGAAGC AGGCTGGAGA CGTGGAGGAG
  421 AACCCTGGAC CTAGATCTAA GGAGAAGAGT GCTTGTCCTA AAGATCCAGC CAAACCTCCG
  481 GCCAAGGCAC AAGTTGTGGG ATGGCCACCG GTGAGATCAT ACCGGAAGAA CGTGATGGTT
  541 TCCTGCCAAA AATCAAGCGG TGGCCCGGAG GCGGCGGCGT TCGTGAAGGT ATCAATGGAC
  601 GGAGCACCGT ACTTGAGGAA AATCGATTTG AGGATGTATA AAGCTAGCAT GGTGAGCAAG
  661 GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAN
  721 GGCTCCGTGA ACGGCCACNA NTTCGAGATC GAGGGCGAGG GCGANGGCCG CNCCTACNAN
  781 GGCACCCAGA NCGCCNAGCT GAANGTGACC ANNGGTGGNC CCCCTGCCCT TNGCCTGGGA
  841 CATNCTGTCC CNTCANTTCA TGTACNGNTC CNNNNNNTAC GTGAANCACC NNGCNNACNT
901 CCCCGANNAC TTGNANNNNN NCCNTNNNNN NNNNN
//
Primer: mRFP-M_R (Pr0674)
Region: insert mid(mCherry2 5',mAID,P2A,HygR 3')
Sequence file: RDB16807_A8Lpg.seq check
>16807_16807_B2IlF_mRFP-M_R_D03_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC CTTGGNNCCG TACATGAACT GAGGGGNNAG
   61 GNTGTCCCAG GCGAAGGGCA GGGGGCCACC CTTGGTCACC TTCAGCTTGG CGGTCTGGGT
  121 GCCCTCGTAG GGGCGGCCCT CGCCCTCGCC CTCGATCTCG AACTCGTGGC CGTTCACGGA
  181 GCCCTCCATG TGCACCTTGA AGCGCATGAA CTCCTTGATG ATGGCCATGT TATCCTCCTC
  241 GCCCTTGCTC ACCATGCTAG CTTTATACAT CCTCAAATCG ATTTTCCTCA AGTACGGTGC
  301 TCCGTCCATT GATACCTTCA CGAACGCCGC CGCCTCCGGG CCACCGCTTG ATTTTTGGCA
  361 GGAAACCATC ACGTTCTTCC GGTATGATCT CACCGGTGGC CATCCCACAA CTTGTGCCTT
  421 GGCCGGAGGT TTGGCTGGAT CTTTAGGACA AGCACTCTTC TCCTTAGATC TAGGTCCAGG
  481 GTTCTCCTCC ACGTCTCCAG CCTGCTTCAG CAGGCTGAAG TTAGTAGCTC CGCTTCCTGA
  541 TTCCTTTGCC CTCGGACGAG TGCTGGGGCG TCGGTTTCCA CTATCGGCGA GTACTTCTAC
  601 ACAGCCATCG GTCCAGACGG CCGCGCTTCT GCGGGCGATT TGTGTACGCC CGACAGTCCC
  661 GGCTCCGGAT CGGACGATTG CGTCGCATCG ACCCTGCGCC CAAGCTGCAT CATCGAAATT
  721 GCCGTCNACC AAGCTCTGAN AGAGTTGGTC AAGANCAATG CGGANCATAT ACGCCCGGAG
  781 CCGCGGCGAT CCTGCAAGCT CCNGANGCCT NCGCTCNAAN TANCGCGNCN GCTGCTNCAT
841 NCANCCNNNN NNNNNN
//
Primer: lacIpro-B_F (Pr0724)
Region: T3 pro,insert 3'(Linker,mCherry2 3')
Sequence file: RDB16807_A8Lph.seq check
>16807_16807_B2IlF_lacIpro-B_F_B05_05_ABI24.ab1
    1 NNNNNNNNNN NNNNCGCNNC GNGTCNGTGA GCGAGGNAGC GGAAGAGCGC CNAATACGCA
   61 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG
  121 ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC
  181 CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC
  241 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCGCGCAATT AACCCTCACT
  301 AAAGGGAACA AAAGCTGGAG CTCCACCGCG GTGGCGGCCG CTCTAGAACT AGTGGATCCG
  361 GCGCCTGCAC CAGCGCCCTT GTACAGCTCG TCCATGCCGC CGGTGGAGTG GCGGCCCTCG
  421 GCGCGTTCGT ACTGTTCCAC GATGGTGTAG TCCTCGTTGT GGGAAAGGAT GTCCAACTTG
  481 ATGTCGACGT TGTAGGCGCC GGGCAGCTGC ACGGGCTTCT TGGCCTTGTA GGTGGTCTTG
  541 ACCTCAGCGT CGTAGTGGCC GCCGTCCTTC AGCTTCAGCC TCTGCTTGAT CTCGCCCTTC
  601 AGGGCGCCGT CCTCGGGGTA CATCCGCTCA GTGGAGGCCT CCCAGCCCAT GGTACGACAC
  661 TGCATTACGG GGCCGTCGGN NGGGAAGTTG GTGCCGCGCA GCTTCACCTT GTAGATGAAC
  721 TCGCCGTCCT GCAGGGAGGA GTCCTGGGTC ACGGTCACCA CGCCGCCGTC CTCGAAGTTC
  781 ATCACGCGCT CCCAATTGAA GCCCTCGGGG AANGACAGCT TCNAGTAGTC GGGGNNGTCG
  841 GCGNNGTGCT TCACGTANGC CTTGGANCCG TACATGAACT GAGGGGANAG GANGTCCCAG
  901 GCGAANGGCA NGGGGNCACC CTNGGNCACC TTCAGCTTNG CGGNCTGGGN GCCNNNNNAN
  961 GGGCGGNCCN NGCCNNNGCC NNCGATCTCG AACTNNNNGN NCNNTCNNNN ANCCNNNCNN
1021 NNGCNNNNTN NNNNCNNANN NNNNNCNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]