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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK346 (Hygro-P2A-mAID-mCherry2)

A plasmid for construction of N-terminal Hygro-P2A-mAID-mCherry2 tagging donors.

Catalog number RDB16807
Resource name pMK346 (Hygro-P2A-mAID-mCherry2)
Clone info. A plasmid for construction of N-terminal Hygro-P2A-mAID-mCherry2 tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Discosoma sp. RFP cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank

External Database
Arabidopsis thaliana AtIAA17 Discosoma sp. RFP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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          Remarks
          The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose).
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          RDB16807 pMK346 (Hygro-P2A-mAID-mCherry2) DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16807_A8Lpp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pUC-M13_-100
          Region: T7 pro, insert 5' (HygR 5')
          Sequence file: RDB16807_A8Lpa.seq check
          >D06157A3_A8LP_2_pUC-M13_-100_F01_16_ABI24.ab1
              1 GGCCGTCAGC GGATTAGTTG GGTACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA
             61 CGGCCAGTGA GCGCGCGTAA TACGACTCAC TATAGGGCGA ATTGGGTACC GGGCCCCCCC
            121 TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGCCACCAT GAAAAAGCCT GAACTCACCG
            181 CGACGTCTGT CGAGAAGTTT CTGATCGAAA AGTTCGACAG CGTCTCCGAC CTGATGCAGC
            241 TCTCGGAGGG CGAAGAATCT CGTGCTTTCA GCTTCGATGT AGGAGGGCGT GGATATGTCC
            301 TGCGGGTAAA TAGCTGCGCC GATGGTTTCT ACAAAGATCG TTATGTTTAT CGGCACTTTG
            361 CATCGGCCGC GCTCCCGATT CCGGAAGTGC TTGACATTGG GGAATTCAGC GAGAGCCTGA
            421 CCTATTGCAT CTCCCGCCGT GCACAGGGTG TCACGTTGCA AGACCTGCCT GAAACCGAAC
            481 TGCCCGCTGT TCTGCAGCCG GTCGCGGAGG CCATGGATGC GATCGCTGCG GCCGATCTTA
            541 GCCAGACGAG CGGGTTCGGC CCATTCGGAC CGCAAGGAAT CGGTCAATAC ACTACATGGC
            601 GTGATTTCAT ATGCGCGATT GCTGATCCCC ATGTGTATCA CTGGCAAACT GTGATGGACG
            661 ACACCGTCAG TGCGTCCGTC GCGCAGGCTC TCGATGAGCT GATGCTTTGG GCCGAGGACT
            721 GCCCCGAAGT CCGGCACCTC GTGCACGCGG ATTTCGGCTC CAACAATGTC CTGACGGACA
            781 ATGGCCGCAT AACAGCGGTC ATTGACTGGA GCGAGGCGAT GTTCGGGGAT TCCCAATACG
            841 AGGTCGCCAA CATCTTCTTC TGGAGGCCGT GGTTGGCTTG TATGGAGCAG CAGACGCGCT
            901 ACTTCGAGCG GAGGCATCCG GAGCTTGCAG GATCGCCGCG GCTCCGGGCG TATATGCTCC
            961 CGCATTGGGT CTTGACCAAA CTCTATCAGA GCTTTGCTTG ACGGCAATTT CGATGATGCA
           1021 GCTTTGAGCG CAGGTTCGGA TGCGAACGCA TCGTCCGAAT CCAGAGCTGG AACCTGCTCC
          1081 GGGCCGTTAC AACAAAATTC GATCTG
          //
          Primer: reverse
          Region: T3 pro, insert 5' (Linker, mCherry2, mAID)
          Sequence file: RDB16807_A8Lpb.seq check
          >D06157A3_A8LP_2_reverse_G01_19_ABI24.ab1
              1 CTTGAAATTT AAACCTCACT AAAAGGGAAC AAAAGCTGGA GCTCCACCGC GGTGGCGGCC
             61 GCTCTAGAAC TAGTGGATCC GGCGCCTGCA CCAGCGCCCT TGTACAGCTC GTCCATGCCG
            121 CCGGTGGAGT GGCGGCCCTC GGCGCGTTCG TACTGTTCCA CGATGGTGTA GTCCTCGTTG
            181 TGGGAAAGGA TGTCCAACTT GATGTCGACG TTGTAGGCGC CGGGCAGCTG CACGGGCTTC
            241 TTGGCCTTGT AGGTGGTCTT GACCTCAGCG TCGTAGTGGC CGCCGTCCTT CAGCTTCAGC
            301 CTCTGCTTGA TCTCGCCCTT CAGGGCGCCG TCCTCGGGGT ACATCCGCTC AGTGGAGGCC
            361 TCCCAGCCCA TGGTACGACA CTGCATTACG GGGCCGTCGG AGGGGAAGTT GGTGCCGCGC
            421 AGCTTCACCT TGTAGATGAA CTCGCCGTCC TGCAGGGAGG AGTCCTGGGT CACGGTCACC
            481 ACGCCGCCGT CCTCGAAGTT CATCACGCGC TCCCAATTGA AGCCCTCGGG GAAGGACAGC
            541 TTCAAGTAGT CGGGGATGTC GGCGGGGTGC TTCACGTAGG CCTTGGAGCC GTACATGAAC
            601 TGAGGGGACA GGATGTCCCA GGCGAAGGGC AGGGGGCCAC CCTTGGTCAC CTTCAGCTTG
            661 GCGGTCTGGG TGCCCTCGTA GGGGCGGCCC TCGCCCTCGC CCTCGATCTC GAACTCGTGG
            721 CCGTTCACGG AGCCCTCCAT GTGCACCTTG AAGCGCATGA ACTCCTTGAT GATGGCCATG
            781 TTATCCTCCT CGCCCTTGCT CACCATGCTA GCTTTATACA TCCTCAAATC GATTTTCCTC
            841 AAGTACGGTG CTCCGTCCAT TGATACCTTC ACGAACGCCG CCGCCTCCGG GCCACCGCTT
            901 GATTTTTGGC AGGAAACCAT CACGTTCTTC CGGTATGATC TCACCGGTGG CCCATCCCAC
            961 AACTTGTGCC CTTGGCCCGG AGGTTTGGGC TGGATCTTTA GACAAGGCAC TCTTCTGTTA
           1021 GATCTAGTCC AGGATCTCCT CAACGTCTCC AGGCCTGCTC CAGCCAAGCC TGAAAGGTTA
          1081 GGTTAGGCAT CCCCGACCTA
          //
          Primer: HygR-C_F
          Region: insert mid (HygR 3', P2A, mAID, mCherry)
          Sequence file: RDB16807_A8Lpc.seq check
          >D06157A3_A8LP_2_HygR-C_F_H01_22_ABI24.ab1
              1 GGGAATTCGG GGGATCCAAT ACGAGGTCGC CACATCTTCT TCTGGAGGCC GTGGTTGGCT
             61 TGTATGGAGC AGCAGACGCG CTACTTCGAG CGGAGGCATC CGGAGCTTGC AGGATCGCCG
            121 CGGCTCCGGG CGTATATGCT CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC
            181 GGCAATTTCG ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA
            241 GCCGGGACTG TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG GACCGATGGC
            301 TGTGTAGAAG TACTCGCCGA TAGTGGAAAC CGACGCCCCA GCACTCGTCC GAGGGCAAAG
            361 GAATCAGGAA GCGGAGCTAC TAACTTCAGC CTGCTGAAGC AGGCTGGAGA CGTGGAGGAG
            421 AACCCTGGAC CTAGATCTAA GGAGAAGAGT GCTTGTCCTA AAGATCCAGC CAAACCTCCG
            481 GCCAAGGCAC AAGTTGTGGG ATGGCCACCG GTGAGATCAT ACCGGAAGAA CGTGATGGTT
            541 TCCTGCCAAA AATCAAGCGG TGGCCCGGAG GCGGCGGCGT TCGTGAAGGT ATCAATGGAC
            601 GGAGCACCGT ACTTGAGGAA AATCGATTTG AGGATGTATA AAGCTAGCAT GGTGAGCAAG
            661 GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAG
            721 GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG
            781 GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCCTGCCCT TCGCCTGGGA
            841 CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT
            901 CCCCGACTAC TTGAAAGCTG TCCTTCCCCG AGGCTTCAAT TGGGAGCGCG TGATGAACTT
            961 CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACC GCGAGTTCAT
           1021 CTACAGGTGA AGCTGCGCGG CACCAACTCC CCACGACGGA CCTCCGTAAT GCAGTGATCT
          1081 GTAATCCTCA AT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

          2021.11.02

          GNP_filter3_RDBDEP_html_211029.pl