Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK345 (Hygro-P2A-mAID-mClover)

A plasmid for construction of N-terminal Hygro-P2A-mAID-mClover tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16806_A8Lpp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 Region: T7 pro, HygR Sequence file: RDB16806_A8Lpa.seq
>D06157A2_A8LP_2_pUC-M13_-100_C01_07_ABI24.ab1     1 GACGGTCAAG CGGATTAGTT GGGTACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG    61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGGTAC CGGGCCCCCC   121 CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGCCACCA TGAAAAAGCC TGAACTCACC   181 GCGACGTCTG TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTCTCCGA CCTGATGCAG   241 CTCTCGGAGG GCGAAGAATC TCGTGCTTTC AGCTTCGATG TAGGAGGGCG TGGATATGTC   301 CTGCGGGTAA ATAGCTGCGC CGATGGTTTC TACAAAGATC GTTATGTTTA TCGGCACTTT   361 GCATCGGCCG CGCTCCCGAT TCCGGAAGTG CTTGACATTG GGGAATTCAG CGAGAGCCTG   421 ACCTATTGCA TCTCCCGCCG TGCACAGGGT GTCACGTTGC AAGACCTGCC TGAAACCGAA   481 CTGCCCGCTG TTCTGCAGCC GGTCGCGGAG GCCATGGATG CGATCGCTGC GGCCGATCTT   541 AGCCAGACGA GCGGGTTCGG CCCATTCGGA CCGCAAGGAA TCGGTCAATA CACTACATGG   601 CGTGATTTCA TATGCGCGAT TGCTGATCCC CATGTGTATC ACTGGCAAAC TGTGATGGAC   661 GACACCGTCA GTGCGTCCGT CGCGCAGGCT CTCGATGAGC TGATGCTTTG GGCCGAGGAC   721 TGCCCCGAAG TCCGGCACCT CGTGCACGCG GATTTCGGCT CCAACAATGT CCTGACGGAC   781 AATGGCCGCA TAACAGCGGT CATTGACTGG AGCGAGGCGA TGTTCGGGGA TTCCCAATAC   841 GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT GTATGGAGCA GCAGACGCGC   901 TACTTCGAGC GGGAGGCATC CGGAGCTTGC AGGATCGCCG CGGCTCCGGG CGTATATGCT   961 CCGCATTGGT CTTGACCCAA CTCTATCAGA GCTTTGCTTG ACGGCAATTT CGATGATGCA  1021 GCTTTGAGCG CAGGTCGAAT GCCGACGGCA TTCGTCGATT CCGAGCTGGA ACTGGTCCGG 1081 GCCGTACACA AACTTCGGTC TCTCG //
Primer: reverse Region: Linker, mClover, mAID Sequence file: RDB16806_A8Lpb.seq
>D06157A2_A8LP_2_reverse_D01_10_ABI24.ab1     1 ATTGGCCATT ACCTCACTAA AAGGGACAAA AGCTGGAGCT CCACCGCGGT GGCGGCCGCT    61 CTAGAACTAG TGGATCCGGC GCCTGCACCA GCGCCCTTGT ACAGCTCGTC CATGCCATGT   121 GTAATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT CTCGTTGGGG   181 TCTTTGCTCA GCTTGGACTG ATGGCTCAGG TAGTGGTTGT CGGGCAGCAG CACGGGGCCG   241 TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCTGCC GTCCTCAACG   301 TTGTGGCGGA TCTTGAAGTT AGCCTTGATG CCGTTCTTCT GCTTGTCGGC CGTGATATAG   361 ACGTTGTGGC TGTTGAAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC GTCCTCCTTG   421 AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA CTTCACCTCG   481 GCGCGGGTCT TGTAGGTACC GTCGTCCTTG AAAGAGATGG TGCGCTCCTG GACGTAGCCT   541 TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA GCGGCTGAAG   601 CAGGCCACGC CGTAGCCGAA GGTGGTCACG AGGGTGGGCC AGGGCACGGG CAGCTTGCCG   661 GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTTGGTGG CATCGCCCTC GCCCTCGCCG   721 CGGACGCTGA ACTTGTGGCC GTTTACGTCG CCGTCCAGCT CGACCAGGAT GGGCACCACC   781 CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATGCTAGCTT TATACATCCT CAAATCGATT   841 TTCCTCAAGT ACGGTGCTCC GTCCATTGAT ACCTTCACGA ACGCCGCCGC CTCCGGGCCA   901 CCGCTTGATT TTTTGGCAGG AAACCATCAC GTTCTTCCGG TATGATCTCA CCGGTGGGCC   961 ATCCCACAAC TTTGTGCCTT TGGCCCGGAG TTTGGCTGGG ATCTTTAGGA CAAGCACTCC  1021 TCTCATTAGA TCTAGGTCAG GATCTCTCAA CGTCTCCAGC CTGCATCAGC AGCTGAGTAG 1081 TAGCATCGGC ATACATGAAC CA //
Primer: HygR-C_F Region: HygR, P2A, mAID, mClover Sequence file: RDB16806_A8Lpc.seq
>D06157A2_A8LP_2_HygR-C_F_E01_13_ABI24.ab1     1 CGTATTTCGG GGATCCATAC GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT    61 GTATGGAGCA GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCGC   121 GGCTCCGGGC GTATATGCTC CGCATTGGTC TTGACCAACT CTATCAGAGC TTGGTTGACG   181 GCAATTTCGA TGATGCAGCT TGGGCGCAGG GTCGATGCGA CGCAATCGTC CGATCCGGAG   241 CCGGGACTGT CGGGCGTACA CAAATCGCCC GCAGAAGCGC GGCCGTCTGG ACCGATGGCT   301 GTGTAGAAGT ACTCGCCGAT AGTGGAAACC GACGCCCCAG CACTCGTCCG AGGGCAAAGG   361 AATCAGGAAG CGGAGCTACT AACTTCAGCC TGCTGAAGCA GGCTGGAGAC GTGGAGGAGA   421 ACCCTGGACC TAGATCTAAG GAGAAGAGTG CTTGTCCTAA AGATCCAGCC AAACCTCCGG   481 CCAAGGCACA AGTTGTGGGA TGGCCACCGG TGAGATCATA CCGGAAGAAC GTGATGGTTT   541 CCTGCCAAAA ATCAAGCGGT GGCCCGGAGG CGGCGGCGTT CGTGAAGGTA TCAATGGACG   601 GAGCACCGTA CTTGAGGAAA ATCGATTTGA GGATGTATAA AGCTAGCATG GTGAGCAAGG   661 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG   721 GCCACAAGTT CAGCGTCCGC GGCGAGGGCG AGGGCGATGC CACCAACGGC AAGCTGACCC   781 TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCT   841 TCGGCTACGG CGTGGGCCTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC   901 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTCTTT CAAGGACGAC   961 GGTACCTACA AGACCCGCGC CGAGGGTGAA GTTTCGAGGG CGACACCCTG ATGGACCGCA  1021 TCGAGCTGAG GGCCATCGAC CTTCCAAGGA AGAACGCTAC ATCCTGAGCA CAGCTGGAAG 1081 TAACCAACTT CGA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2021.11.02

GNP_filter3_RDBDEP_html_211029.pl