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pMK345 (Hygro-P2A-mAID-mClover) (#RDB16806)

A plasmid for construction of N-terminal Hygro-P2A-mAID-mClover tagging donors.

Clone info. A plasmid for construction of N-terminal Hygro-P2A-mAID-mClover tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Kanemaki, Masato |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。

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RDB16806 pMK345 (Hygro-P2A-mAID-mClover) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMK345 (Hygro-P2A-mAID-mClover) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16806).

Reference section:

Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16806_A8Lpp1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310)
Region: T7 pro,insert 5'(HygR 5')
Sequence file: RDB16806_A8Lpd.seq check
>D06157A2_A8LP_2_pUC-M13_-100_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNTT GGGTANGCNN GGNNTTTCCC NGTCACGACG TTGTAAAACG
   61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGGTAC CGGGCCCCCC
  121 CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGCCACCA TGAAAAAGCC TGAACTCACC
  181 GCGACGTCTG TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTCTCCGA CCTGATGCAG
  241 CTCTCGGAGG GCGAAGAATC TCGTGCTTTC AGCTTCGATG TAGGAGGGCG TGGATATGTC
  301 CTGCGGGTAA ATAGCTGCGC CGATGGTTTC TACAAAGATC GTTATGTTTA TCGGCACTTT
  361 GCATCGGCCG CGCTCCCGAT TCCGGAAGTG CTTGACATTG GGGAATTCAG CGAGAGCCTG
  421 ACCTATTGCA TCTCCCGCCG TGCACAGGGT GTCACGTTGC AAGACCTGCC TGAAACCGAA
  481 CTGCCCGCTG TTCTGCAGCC GGTCGCGGAG GCCATGGATG CGATCGCTGC GGCCGATCTT
  541 AGCCAGACGA GCGGGTTCGG CCCATTCGGA CCGCAAGGAA TCGGTCAATA CACTACATGG
  601 CGTGATTTCA TATGCGCGAT TGCTGATCCC CATGTGTATC ACTGGCAAAC TGTGATGGAC
  661 GACACCGTCA GTGCGTCCGT CGCGCANGCT CTCGATGANC TGATGCTTTG GGCCGANGAC
  721 TGCCCCGAAG TCCGGCACCT CGTGCACGCG GATTTCGGCT CCNACAATGT CCTGACGGAC
  781 AATGGCCGCA TAACAGCGGT CATTGACTGG AGCGAGGCGA TGTTCGGGGN NTCCCNATAC
  841 GANGNCGCCN NNNTCTTCNT CTGGANGCCG NGGTNNNNTN GTATNGNNCA NCANANNCNN
901 NNCTTNNNNN NGNANNNNTC CGGANNNNGN NNNATCNNNN NNNNNN
//
Primer: reverse (Pr0099)
Region: insert 3'(Linker,mClover(A206K) 3')
Sequence file: RDB16806_A8Lpe.seq check
>D06157A2_A8LP_2_reverse_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGGNNNNNA AGCTGGAGCT CCACCGCGGT GGCGGCCGCT
   61 CTAGAACTAG TGGATCCGGC GCCTGCACCA GCGCCCTTGT ACAGCTCGTC CATGCCATGT
  121 GTAATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT CTCGTTGGGG
  181 TCTTTGCTCA GCTTGGACTG ATGGCTCAGG TAGTGGTTGT CGGGCAGCAG CACGGGGCCG
  241 TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCTGCC GTCCTCAACG
  301 TTGTGGCGGA TCTTGAAGTT AGCCTTGATG CCGTTCTTCT GCTTGTCGGC CGTGATATAG
  361 ACGTTGTGGC TGTTGAAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC GTCCTCCTTG
  421 AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA CTTCACCTCG
  481 GCGCGGGTCT TGTAGGTACC GTCGTCCTTG AAAGAGATGG TGCGCTCCTG GACGTAGCCT
  541 TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA GCGGCTGAAG
  601 CAGGCCACGC CGTAGCCGAA GGTGGTCACG AGGGTGGGCC AGGGCACGGG CAGCTTGCCG
  661 GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTTGGTGG CATCGCCCTC GCCCTCGCCG
  721 CGGANGCTGA ACTTGTGGCC GTTTACGTCG CCGTCCAGCT CGACCAGGAT GGGCACCACC
  781 CCGGTGAACA GCTCCTCGCC CTTGCTCACC NTGCTAGCTT TATACATCCT CNAATCGANT
  841 TTCCTCNAGT ACGGNGCTNC NNCCNTTGAT ANCNTCACGA ACGCNNCCGC NNNCNGNNCA
901 CCNNTNNNNT TTTNGNCNNN AAANNNTCNC NNNCNTNCNG NNNNNNNNNN
//
Primer: HygR-C_F (Pr0501)
Region: insert mid(HygR,P2A,mAID,mClover(A206K) 5')
Sequence file: RDB16806_A8Lpf.seq check
>D06157A2_A8LP_2_HygR-C_F_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GNNGTCGCCN ACNTCTTCTT CTGGAGGCCG TGGTTGGCTT
   61 GTATGGAGCA GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCGC
  121 GGCTCCGGGC GTATATGCTC CGCATTGGTC TTGACCAACT CTATCAGAGC TTGGTTGACG
  181 GCAATTTCGA TGATGCAGCT TGGGCGCAGG GTCGATGCGA CGCAATCGTC CGATCCGGAG
  241 CCGGGACTGT CGGGCGTACA CAAATCGCCC GCAGAAGCGC GGCCGTCTGG ACCGATGGCT
  301 GTGTAGAAGT ACTCGCCGAT AGTGGAAACC GACGCCCCAG CACTCGTCCG AGGGCAAAGG
  361 AATCAGGAAG CGGAGCTACT AACTTCAGCC TGCTGAAGCA GGCTGGAGAC GTGGAGGAGA
  421 ACCCTGGACC TAGATCTAAG GAGAAGAGTG CTTGTCCTAA AGATCCAGCC AAACCTCCGG
  481 CCAAGGCACA AGTTGTGGGA TGGCCACCGG TGAGATCATA CCGGAAGAAC GTGATGGTTT
  541 CCTGCCAAAA ATCAAGCGGT GGCCCGGAGG CGGCGGCGTT CGTGAAGGTA TCAATGGACG
  601 GAGCACCGTA CTTGAGGAAA ATCGATTTGA GGATGTATAA AGCTAGCATG GTGAGCAAGG
  661 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG
  721 GCCACAAGTT CAGCGTCCGC GGCGAGGGCG ANGGCGATGC CACCAACGGC AAGCTGACCC
  781 TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGNGCCCTG NCCCACCNTC GNGANCACCT
  841 TCNGNTACNG CGNGGNNCTG CTTCANCCGN NNCCCCGANC ACNTNAANCA GCACGNNTTC
  901 TTNNNGTNCG NNNTNCCNNA NNNNTACNNN CNNNNNCGNN NNNTNNNNTN NNNNNNNNAN
961 NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]