Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK345 (Hygro-P2A-mAID-mClover)

A plasmid for construction of N-terminal Hygro-P2A-mAID-mClover tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16806_A8Lpp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310) Region: T7 pro,insert 5'(HygR 5') Sequence file: RDB16806_A8Lpd.seq
>D06157A2_A8LP_2_pUC-M13_-100_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNTT GGGTANGCNN GGNNTTTCCC NGTCACGACG TTGTAAAACG    61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGGTAC CGGGCCCCCC   121 CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGCCACCA TGAAAAAGCC TGAACTCACC   181 GCGACGTCTG TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTCTCCGA CCTGATGCAG   241 CTCTCGGAGG GCGAAGAATC TCGTGCTTTC AGCTTCGATG TAGGAGGGCG TGGATATGTC   301 CTGCGGGTAA ATAGCTGCGC CGATGGTTTC TACAAAGATC GTTATGTTTA TCGGCACTTT   361 GCATCGGCCG CGCTCCCGAT TCCGGAAGTG CTTGACATTG GGGAATTCAG CGAGAGCCTG   421 ACCTATTGCA TCTCCCGCCG TGCACAGGGT GTCACGTTGC AAGACCTGCC TGAAACCGAA   481 CTGCCCGCTG TTCTGCAGCC GGTCGCGGAG GCCATGGATG CGATCGCTGC GGCCGATCTT   541 AGCCAGACGA GCGGGTTCGG CCCATTCGGA CCGCAAGGAA TCGGTCAATA CACTACATGG   601 CGTGATTTCA TATGCGCGAT TGCTGATCCC CATGTGTATC ACTGGCAAAC TGTGATGGAC   661 GACACCGTCA GTGCGTCCGT CGCGCANGCT CTCGATGANC TGATGCTTTG GGCCGANGAC   721 TGCCCCGAAG TCCGGCACCT CGTGCACGCG GATTTCGGCT CCNACAATGT CCTGACGGAC   781 AATGGCCGCA TAACAGCGGT CATTGACTGG AGCGAGGCGA TGTTCGGGGN NTCCCNATAC   841 GANGNCGCCN NNNTCTTCNT CTGGANGCCG NGGTNNNNTN GTATNGNNCA NCANANNCNN 901 NNCTTNNNNN NGNANNNNTC CGGANNNNGN NNNATCNNNN NNNNNN //
Primer: reverse (Pr0099) Region: insert 3'(Linker,mClover(A206K) 3') Sequence file: RDB16806_A8Lpe.seq
>D06157A2_A8LP_2_reverse_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGGNNNNNA AGCTGGAGCT CCACCGCGGT GGCGGCCGCT    61 CTAGAACTAG TGGATCCGGC GCCTGCACCA GCGCCCTTGT ACAGCTCGTC CATGCCATGT   121 GTAATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT CTCGTTGGGG   181 TCTTTGCTCA GCTTGGACTG ATGGCTCAGG TAGTGGTTGT CGGGCAGCAG CACGGGGCCG   241 TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCTGCC GTCCTCAACG   301 TTGTGGCGGA TCTTGAAGTT AGCCTTGATG CCGTTCTTCT GCTTGTCGGC CGTGATATAG   361 ACGTTGTGGC TGTTGAAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC GTCCTCCTTG   421 AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA CTTCACCTCG   481 GCGCGGGTCT TGTAGGTACC GTCGTCCTTG AAAGAGATGG TGCGCTCCTG GACGTAGCCT   541 TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA GCGGCTGAAG   601 CAGGCCACGC CGTAGCCGAA GGTGGTCACG AGGGTGGGCC AGGGCACGGG CAGCTTGCCG   661 GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTTGGTGG CATCGCCCTC GCCCTCGCCG   721 CGGANGCTGA ACTTGTGGCC GTTTACGTCG CCGTCCAGCT CGACCAGGAT GGGCACCACC   781 CCGGTGAACA GCTCCTCGCC CTTGCTCACC NTGCTAGCTT TATACATCCT CNAATCGANT   841 TTCCTCNAGT ACGGNGCTNC NNCCNTTGAT ANCNTCACGA ACGCNNCCGC NNNCNGNNCA 901 CCNNTNNNNT TTTNGNCNNN AAANNNTCNC NNNCNTNCNG NNNNNNNNNN //
Primer: HygR-C_F (Pr0501) Region: insert mid(HygR,P2A,mAID,mClover(A206K) 5') Sequence file: RDB16806_A8Lpf.seq
>D06157A2_A8LP_2_HygR-C_F_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GNNGTCGCCN ACNTCTTCTT CTGGAGGCCG TGGTTGGCTT    61 GTATGGAGCA GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCGC   121 GGCTCCGGGC GTATATGCTC CGCATTGGTC TTGACCAACT CTATCAGAGC TTGGTTGACG   181 GCAATTTCGA TGATGCAGCT TGGGCGCAGG GTCGATGCGA CGCAATCGTC CGATCCGGAG   241 CCGGGACTGT CGGGCGTACA CAAATCGCCC GCAGAAGCGC GGCCGTCTGG ACCGATGGCT   301 GTGTAGAAGT ACTCGCCGAT AGTGGAAACC GACGCCCCAG CACTCGTCCG AGGGCAAAGG   361 AATCAGGAAG CGGAGCTACT AACTTCAGCC TGCTGAAGCA GGCTGGAGAC GTGGAGGAGA   421 ACCCTGGACC TAGATCTAAG GAGAAGAGTG CTTGTCCTAA AGATCCAGCC AAACCTCCGG   481 CCAAGGCACA AGTTGTGGGA TGGCCACCGG TGAGATCATA CCGGAAGAAC GTGATGGTTT   541 CCTGCCAAAA ATCAAGCGGT GGCCCGGAGG CGGCGGCGTT CGTGAAGGTA TCAATGGACG   601 GAGCACCGTA CTTGAGGAAA ATCGATTTGA GGATGTATAA AGCTAGCATG GTGAGCAAGG   661 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG   721 GCCACAAGTT CAGCGTCCGC GGCGAGGGCG ANGGCGATGC CACCAACGGC AAGCTGACCC   781 TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGNGCCCTG NCCCACCNTC GNGANCACCT   841 TCNGNTACNG CGNGGNNCTG CTTCANCCGN NNCCCCGANC ACNTNAANCA GCACGNNTTC   901 TTNNNGTNCG NNNTNCCNNA NNNNTACNNN CNNNNNCGNN NNNTNNNNTN NNNNNNNNAN 961 NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.10.05

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