Sequencing and PCR primers for primer information.
A plasmid for construction of N-terminal Hygro-P2A-mAID-mClover tagging donors.
| Catalog number | RDB16806 |
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| Resource name | pMK345 (Hygro-P2A-mAID-mClover) |
| Clone info. | A plasmid for construction of N-terminal Hygro-P2A-mAID-mClover tagging donors. |
| Vector backbone | pBluescript (plasmid) |
| Size of vector backbone | 2.9 kb |
| Selectable markers | Am^r (E. coli) |
| Gene/insert name | Arabidopsis thaliana AtIAA17 cDNA Aequorea victoria GFP cDNA |
| Depositor | Kanemaki, Masato | |
| Other clones in our bank | Arabidopsis thaliana AXR3 (KEGG orthology K14484) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB16806 | pMK345 (Hygro-P2A-mAID-mClover) | DNA solution |
Electronic file
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Auxin Inducible Degron (AID) System (English text) |
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| Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB16806_A8Lpp1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pUC-M13_-100 | RDB16806_A8Lpa.seq |
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>D06157A2_A8LP_2_pUC-M13_-100_C01_07_ABI24.ab1 1 GACGGTCAAG CGGATTAGTT GGGTACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG 61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGGTAC CGGGCCCCCC 121 CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGCCACCA TGAAAAAGCC TGAACTCACC 181 GCGACGTCTG TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTCTCCGA CCTGATGCAG 241 CTCTCGGAGG GCGAAGAATC TCGTGCTTTC AGCTTCGATG TAGGAGGGCG TGGATATGTC 301 CTGCGGGTAA ATAGCTGCGC CGATGGTTTC TACAAAGATC GTTATGTTTA TCGGCACTTT 361 GCATCGGCCG CGCTCCCGAT TCCGGAAGTG CTTGACATTG GGGAATTCAG CGAGAGCCTG 421 ACCTATTGCA TCTCCCGCCG TGCACAGGGT GTCACGTTGC AAGACCTGCC TGAAACCGAA 481 CTGCCCGCTG TTCTGCAGCC GGTCGCGGAG GCCATGGATG CGATCGCTGC GGCCGATCTT 541 AGCCAGACGA GCGGGTTCGG CCCATTCGGA CCGCAAGGAA TCGGTCAATA CACTACATGG 601 CGTGATTTCA TATGCGCGAT TGCTGATCCC CATGTGTATC ACTGGCAAAC TGTGATGGAC 661 GACACCGTCA GTGCGTCCGT CGCGCAGGCT CTCGATGAGC TGATGCTTTG GGCCGAGGAC 721 TGCCCCGAAG TCCGGCACCT CGTGCACGCG GATTTCGGCT CCAACAATGT CCTGACGGAC 781 AATGGCCGCA TAACAGCGGT CATTGACTGG AGCGAGGCGA TGTTCGGGGA TTCCCAATAC 841 GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT GTATGGAGCA GCAGACGCGC 901 TACTTCGAGC GGGAGGCATC CGGAGCTTGC AGGATCGCCG CGGCTCCGGG CGTATATGCT 961 CCGCATTGGT CTTGACCCAA CTCTATCAGA GCTTTGCTTG ACGGCAATTT CGATGATGCA 1021 GCTTTGAGCG CAGGTCGAAT GCCGACGGCA TTCGTCGATT CCGAGCTGGA ACTGGTCCGG 1081 GCCGTACACA AACTTCGGTC TCTCG // |
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| Primer: reverse | RDB16806_A8Lpb.seq |
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>D06157A2_A8LP_2_reverse_D01_10_ABI24.ab1 1 ATTGGCCATT ACCTCACTAA AAGGGACAAA AGCTGGAGCT CCACCGCGGT GGCGGCCGCT 61 CTAGAACTAG TGGATCCGGC GCCTGCACCA GCGCCCTTGT ACAGCTCGTC CATGCCATGT 121 GTAATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT CTCGTTGGGG 181 TCTTTGCTCA GCTTGGACTG ATGGCTCAGG TAGTGGTTGT CGGGCAGCAG CACGGGGCCG 241 TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCTGCC GTCCTCAACG 301 TTGTGGCGGA TCTTGAAGTT AGCCTTGATG CCGTTCTTCT GCTTGTCGGC CGTGATATAG 361 ACGTTGTGGC TGTTGAAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC GTCCTCCTTG 421 AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA CTTCACCTCG 481 GCGCGGGTCT TGTAGGTACC GTCGTCCTTG AAAGAGATGG TGCGCTCCTG GACGTAGCCT 541 TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA GCGGCTGAAG 601 CAGGCCACGC CGTAGCCGAA GGTGGTCACG AGGGTGGGCC AGGGCACGGG CAGCTTGCCG 661 GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTTGGTGG CATCGCCCTC GCCCTCGCCG 721 CGGACGCTGA ACTTGTGGCC GTTTACGTCG CCGTCCAGCT CGACCAGGAT GGGCACCACC 781 CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATGCTAGCTT TATACATCCT CAAATCGATT 841 TTCCTCAAGT ACGGTGCTCC GTCCATTGAT ACCTTCACGA ACGCCGCCGC CTCCGGGCCA 901 CCGCTTGATT TTTTGGCAGG AAACCATCAC GTTCTTCCGG TATGATCTCA CCGGTGGGCC 961 ATCCCACAAC TTTGTGCCTT TGGCCCGGAG TTTGGCTGGG ATCTTTAGGA CAAGCACTCC 1021 TCTCATTAGA TCTAGGTCAG GATCTCTCAA CGTCTCCAGC CTGCATCAGC AGCTGAGTAG 1081 TAGCATCGGC ATACATGAAC CA // |
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| Primer: HygR-C_F | RDB16806_A8Lpc.seq |
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>D06157A2_A8LP_2_HygR-C_F_E01_13_ABI24.ab1 1 CGTATTTCGG GGATCCATAC GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT 61 GTATGGAGCA GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCGC 121 GGCTCCGGGC GTATATGCTC CGCATTGGTC TTGACCAACT CTATCAGAGC TTGGTTGACG 181 GCAATTTCGA TGATGCAGCT TGGGCGCAGG GTCGATGCGA CGCAATCGTC CGATCCGGAG 241 CCGGGACTGT CGGGCGTACA CAAATCGCCC GCAGAAGCGC GGCCGTCTGG ACCGATGGCT 301 GTGTAGAAGT ACTCGCCGAT AGTGGAAACC GACGCCCCAG CACTCGTCCG AGGGCAAAGG 361 AATCAGGAAG CGGAGCTACT AACTTCAGCC TGCTGAAGCA GGCTGGAGAC GTGGAGGAGA 421 ACCCTGGACC TAGATCTAAG GAGAAGAGTG CTTGTCCTAA AGATCCAGCC AAACCTCCGG 481 CCAAGGCACA AGTTGTGGGA TGGCCACCGG TGAGATCATA CCGGAAGAAC GTGATGGTTT 541 CCTGCCAAAA ATCAAGCGGT GGCCCGGAGG CGGCGGCGTT CGTGAAGGTA TCAATGGACG 601 GAGCACCGTA CTTGAGGAAA ATCGATTTGA GGATGTATAA AGCTAGCATG GTGAGCAAGG 661 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG 721 GCCACAAGTT CAGCGTCCGC GGCGAGGGCG AGGGCGATGC CACCAACGGC AAGCTGACCC 781 TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCT 841 TCGGCTACGG CGTGGGCCTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC 901 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTCTTT CAAGGACGAC 961 GGTACCTACA AGACCCGCGC CGAGGGTGAA GTTTCGAGGG CGACACCCTG ATGGACCGCA 1021 TCGAGCTGAG GGCCATCGAC CTTCCAAGGA AGAACGCTAC ATCCTGAGCA CAGCTGGAAG 1081 TAACCAACTT CGA // |
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Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
2019.07.12
GNP_filter3_RDBDEP_html_190431.pl