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pMK345 (Hygro-P2A-mAID-mClover)

A plasmid for construction of N-terminal Hygro-P2A-mAID-mClover tagging donors.

Catalog number RDB16806
Resource name pMK345 (Hygro-P2A-mAID-mClover)
Clone info. A plasmid for construction of N-terminal Hygro-P2A-mAID-mClover tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank

External Database
Arabidopsis thaliana AtIAA17 Aequorea victoria GFP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Schedule A (GFP-A) [Link]
          Remarks
          Please use Schedule A (GFP-A) additionally.
          Distribution information (domestic users) [open/close]


          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16806 pMK345 (Hygro-P2A-mAID-mClover) DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16806_A8Lpp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pUC-M13_-100
          Region: T7 pro, HygR
          Sequence file: RDB16806_A8Lpa.seq check
          >D06157A2_A8LP_2_pUC-M13_-100_C01_07_ABI24.ab1
              1 GACGGTCAAG CGGATTAGTT GGGTACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG
             61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGGTAC CGGGCCCCCC
            121 CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGCCACCA TGAAAAAGCC TGAACTCACC
            181 GCGACGTCTG TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTCTCCGA CCTGATGCAG
            241 CTCTCGGAGG GCGAAGAATC TCGTGCTTTC AGCTTCGATG TAGGAGGGCG TGGATATGTC
            301 CTGCGGGTAA ATAGCTGCGC CGATGGTTTC TACAAAGATC GTTATGTTTA TCGGCACTTT
            361 GCATCGGCCG CGCTCCCGAT TCCGGAAGTG CTTGACATTG GGGAATTCAG CGAGAGCCTG
            421 ACCTATTGCA TCTCCCGCCG TGCACAGGGT GTCACGTTGC AAGACCTGCC TGAAACCGAA
            481 CTGCCCGCTG TTCTGCAGCC GGTCGCGGAG GCCATGGATG CGATCGCTGC GGCCGATCTT
            541 AGCCAGACGA GCGGGTTCGG CCCATTCGGA CCGCAAGGAA TCGGTCAATA CACTACATGG
            601 CGTGATTTCA TATGCGCGAT TGCTGATCCC CATGTGTATC ACTGGCAAAC TGTGATGGAC
            661 GACACCGTCA GTGCGTCCGT CGCGCAGGCT CTCGATGAGC TGATGCTTTG GGCCGAGGAC
            721 TGCCCCGAAG TCCGGCACCT CGTGCACGCG GATTTCGGCT CCAACAATGT CCTGACGGAC
            781 AATGGCCGCA TAACAGCGGT CATTGACTGG AGCGAGGCGA TGTTCGGGGA TTCCCAATAC
            841 GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT GTATGGAGCA GCAGACGCGC
            901 TACTTCGAGC GGGAGGCATC CGGAGCTTGC AGGATCGCCG CGGCTCCGGG CGTATATGCT
            961 CCGCATTGGT CTTGACCCAA CTCTATCAGA GCTTTGCTTG ACGGCAATTT CGATGATGCA
           1021 GCTTTGAGCG CAGGTCGAAT GCCGACGGCA TTCGTCGATT CCGAGCTGGA ACTGGTCCGG
          1081 GCCGTACACA AACTTCGGTC TCTCG
          //
          Primer: reverse
          Region: Linker, mClover, mAID
          Sequence file: RDB16806_A8Lpb.seq check
          >D06157A2_A8LP_2_reverse_D01_10_ABI24.ab1
              1 ATTGGCCATT ACCTCACTAA AAGGGACAAA AGCTGGAGCT CCACCGCGGT GGCGGCCGCT
             61 CTAGAACTAG TGGATCCGGC GCCTGCACCA GCGCCCTTGT ACAGCTCGTC CATGCCATGT
            121 GTAATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT CTCGTTGGGG
            181 TCTTTGCTCA GCTTGGACTG ATGGCTCAGG TAGTGGTTGT CGGGCAGCAG CACGGGGCCG
            241 TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCTGCC GTCCTCAACG
            301 TTGTGGCGGA TCTTGAAGTT AGCCTTGATG CCGTTCTTCT GCTTGTCGGC CGTGATATAG
            361 ACGTTGTGGC TGTTGAAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC GTCCTCCTTG
            421 AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA CTTCACCTCG
            481 GCGCGGGTCT TGTAGGTACC GTCGTCCTTG AAAGAGATGG TGCGCTCCTG GACGTAGCCT
            541 TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA GCGGCTGAAG
            601 CAGGCCACGC CGTAGCCGAA GGTGGTCACG AGGGTGGGCC AGGGCACGGG CAGCTTGCCG
            661 GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTTGGTGG CATCGCCCTC GCCCTCGCCG
            721 CGGACGCTGA ACTTGTGGCC GTTTACGTCG CCGTCCAGCT CGACCAGGAT GGGCACCACC
            781 CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATGCTAGCTT TATACATCCT CAAATCGATT
            841 TTCCTCAAGT ACGGTGCTCC GTCCATTGAT ACCTTCACGA ACGCCGCCGC CTCCGGGCCA
            901 CCGCTTGATT TTTTGGCAGG AAACCATCAC GTTCTTCCGG TATGATCTCA CCGGTGGGCC
            961 ATCCCACAAC TTTGTGCCTT TGGCCCGGAG TTTGGCTGGG ATCTTTAGGA CAAGCACTCC
           1021 TCTCATTAGA TCTAGGTCAG GATCTCTCAA CGTCTCCAGC CTGCATCAGC AGCTGAGTAG
          1081 TAGCATCGGC ATACATGAAC CA
          //
          Primer: HygR-C_F
          Region: HygR, P2A, mAID, mClover
          Sequence file: RDB16806_A8Lpc.seq check
          >D06157A2_A8LP_2_HygR-C_F_E01_13_ABI24.ab1
              1 CGTATTTCGG GGATCCATAC GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT
             61 GTATGGAGCA GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCGC
            121 GGCTCCGGGC GTATATGCTC CGCATTGGTC TTGACCAACT CTATCAGAGC TTGGTTGACG
            181 GCAATTTCGA TGATGCAGCT TGGGCGCAGG GTCGATGCGA CGCAATCGTC CGATCCGGAG
            241 CCGGGACTGT CGGGCGTACA CAAATCGCCC GCAGAAGCGC GGCCGTCTGG ACCGATGGCT
            301 GTGTAGAAGT ACTCGCCGAT AGTGGAAACC GACGCCCCAG CACTCGTCCG AGGGCAAAGG
            361 AATCAGGAAG CGGAGCTACT AACTTCAGCC TGCTGAAGCA GGCTGGAGAC GTGGAGGAGA
            421 ACCCTGGACC TAGATCTAAG GAGAAGAGTG CTTGTCCTAA AGATCCAGCC AAACCTCCGG
            481 CCAAGGCACA AGTTGTGGGA TGGCCACCGG TGAGATCATA CCGGAAGAAC GTGATGGTTT
            541 CCTGCCAAAA ATCAAGCGGT GGCCCGGAGG CGGCGGCGTT CGTGAAGGTA TCAATGGACG
            601 GAGCACCGTA CTTGAGGAAA ATCGATTTGA GGATGTATAA AGCTAGCATG GTGAGCAAGG
            661 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG
            721 GCCACAAGTT CAGCGTCCGC GGCGAGGGCG AGGGCGATGC CACCAACGGC AAGCTGACCC
            781 TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCT
            841 TCGGCTACGG CGTGGGCCTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC
            901 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTCTTT CAAGGACGAC
            961 GGTACCTACA AGACCCGCGC CGAGGGTGAA GTTTCGAGGG CGACACCCTG ATGGACCGCA
           1021 TCGAGCTGAG GGCCATCGAC CTTCCAAGGA AGAACGCTAC ATCCTGAGCA CAGCTGGAAG
          1081 TAACCAACTT CGA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

          2021.11.02

          GNP_filter3_RDBDEP_html_211029.pl