Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK277 (mClover-NeoR)

A plasmid for construction of C-terminal mClover tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

Ordering

Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

References and tips

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13920_A6B8p1_v2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB13920_A6B8a_2.seq
>D03070A04_A6B8_1_M13(-40)_A02_02.ab1     1 AAGCAGGACG CGCGTATACG ACTCACTATA GGGCGAATTG GAGCTCGGAT CCGGTGCAGG    61 CGCCGCTAGC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT   121 CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTC CGCGGCGAGG GCGAGGGCGA   181 TGCCACCAAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC   241 CTGGCCCACC CTCGTGACCA CCTTCGGCTA CGGCGTGGCC TGCTTCAGCC GCTACCCCGA   301 CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG   361 CACCATCTCT TTCAAGGACG ACGGTACCTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG   421 CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT   481 CCTGGGGCAC AAGCTGGAGT ACAACTTCAA CAGCCACAAC GTCTATATCA CGGCCGACAA   541 GCAGAAGAAC GGCATCAAGG CTAACTTCAA GATCCGCCAC AACGTTGAGG ACGGCAGCGT   601 GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC   661 CGACAACCAC TACCTGAGCC ATCAGTCCAA GCTGAGCAAA GACCCCAACG AGAAGCGCGA   721 TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATT ACACATGGCA TGGACGAGCT   781 GTACAAGTAA TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT   841 TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT   901 TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA   961 TTTCACAAAT AAAGGCATTT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAACTCATCC  1021 ATGTATCTAC GCGTAAATGT AAGCGTCATA TTTGTAAATC CCGTTAACTT GTAATTCAGC  1081 TCATTTTTAC CAATAGCCGA ATCGCAATTC CCGTTATTAA ATCCAAAGAA CTATGGACAG //
Primer: SV40pro_F_V2 Sequence file: RDB13920_A6B8b_2.seq
>D03070A04_A6B8_1_SV40pro_F_V2_G09_21.ab1     1 GTGGGCTTGT GGAGCTAGGC TTTTGCAAGA TCGATCAAGA GACAGGATGA GGATCGTTTC    61 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT   121 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT   181 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC   241 TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG   301 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC   361 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA   421 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC   481 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG   541 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG   601 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA   661 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG   721 ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT   781 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTT CTATCGCCTT   841 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTTCGAAA TGACCGACCA AGCGACGCCC   901 AACCTGCCAT CACGAGATTT CGATTCACGC GCTTCTATGA AAGTGGGCTT CGATCGTTTT   961 CGGACGCGCT GAATGATCTT CAGCGCGGGG ATCTCATGCT GGAAGTCCTC GCCAACCTAA 1021 GGAGATAACT GACACGGAGG AACAATACGT ATGCATCGGC TATGACGGCC ATT //
Primer: M13R Sequence file: RDB13920_A6B8c_2.seq
>D03070A04_A6B8_1_M13R_A01_01.ab1     1 GTCGGCATTA CCTCACTAAA GGGAACAAAA GCTGGGTACC GGGCCCCCCC TCGAGGGATC    61 CAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC   121 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC   181 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA   241 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT   301 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA   361 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA   421 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG   481 CGGTCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC   541 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG   601 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA   661 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC   721 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA   781 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC   841 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG   901 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC   961 AGGGCACCGG GACAGGTCGG GTCTTGACAA AAAGAACCCG GGGCGCCCCT GCGCTGACAG  1021 CCGGAACACG CGGGCATCAG AGCAGCGAAT TGTCTGTTTG TGCCCAGTTC ATAGCGGATA  1081 GCCTCTCCAC CCAAGCGCCG AGACCTGGCG TGCATCAATC TTGTTCAATT CCAATGGCGA 1141 TAG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl