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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK277 (mClover-NeoR)

A plasmid for construction of C-terminal mClover tagging donors.

Catalog number RDB13920
Resource name pMK277 (mClover-NeoR)
Alternative name mClover-NeoR,1001
Clone info. A plasmid for construction of C-terminal mClover tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone. Please note that this plasmid does not contain the loxP sites, which are intended for removal of the marker.
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Schedule A (GFP-A) [Link]
            Remarks
            Please use Schedule A (GFP-A) additionally.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            Schedule A(GFP-A, 英語版のみ) [Link]
            備考
            Schedule A(GFP-A, 英語版のみ)が追加で必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB13920 pMK277 (mClover-NeoR) DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Auxin Inducible Degron (AID) System (English text)
            Featured content Auxin Inducible Degron (AID) System (Japanese text)
            Featured content Genome Editing (English text)
            Featured content Genome Editing (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13920_A6B8p1_v2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: M13_-40
            Sequence file: RDB13920_A6B8a_2.seq check
            >D03070A04_A6B8_1_M13(-40)_A02_02.ab1
                1 AAGCAGGACG CGCGTATACG ACTCACTATA GGGCGAATTG GAGCTCGGAT CCGGTGCAGG
               61 CGCCGCTAGC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT
              121 CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTC CGCGGCGAGG GCGAGGGCGA
              181 TGCCACCAAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC
              241 CTGGCCCACC CTCGTGACCA CCTTCGGCTA CGGCGTGGCC TGCTTCAGCC GCTACCCCGA
              301 CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG
              361 CACCATCTCT TTCAAGGACG ACGGTACCTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG
              421 CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT
              481 CCTGGGGCAC AAGCTGGAGT ACAACTTCAA CAGCCACAAC GTCTATATCA CGGCCGACAA
              541 GCAGAAGAAC GGCATCAAGG CTAACTTCAA GATCCGCCAC AACGTTGAGG ACGGCAGCGT
              601 GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC
              661 CGACAACCAC TACCTGAGCC ATCAGTCCAA GCTGAGCAAA GACCCCAACG AGAAGCGCGA
              721 TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATT ACACATGGCA TGGACGAGCT
              781 GTACAAGTAA TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT
              841 TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT
              901 TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA
              961 TTTCACAAAT AAAGGCATTT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAACTCATCC
             1021 ATGTATCTAC GCGTAAATGT AAGCGTCATA TTTGTAAATC CCGTTAACTT GTAATTCAGC
             1081 TCATTTTTAC CAATAGCCGA ATCGCAATTC CCGTTATTAA ATCCAAAGAA CTATGGACAG
            //
            Primer: SV40pro_F_V2
            Sequence file: RDB13920_A6B8b_2.seq check
            >D03070A04_A6B8_1_SV40pro_F_V2_G09_21.ab1
                1 GTGGGCTTGT GGAGCTAGGC TTTTGCAAGA TCGATCAAGA GACAGGATGA GGATCGTTTC
               61 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT
              121 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT
              181 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC
              241 TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG
              301 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC
              361 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA
              421 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC
              481 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG
              541 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG
              601 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA
              661 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG
              721 ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT
              781 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTT CTATCGCCTT
              841 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTTCGAAA TGACCGACCA AGCGACGCCC
              901 AACCTGCCAT CACGAGATTT CGATTCACGC GCTTCTATGA AAGTGGGCTT CGATCGTTTT
              961 CGGACGCGCT GAATGATCTT CAGCGCGGGG ATCTCATGCT GGAAGTCCTC GCCAACCTAA
            1021 GGAGATAACT GACACGGAGG AACAATACGT ATGCATCGGC TATGACGGCC ATT
            //
            Primer: M13R
            Sequence file: RDB13920_A6B8c_2.seq check
            >D03070A04_A6B8_1_M13R_A01_01.ab1
                1 GTCGGCATTA CCTCACTAAA GGGAACAAAA GCTGGGTACC GGGCCCCCCC TCGAGGGATC
               61 CAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC
              121 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC
              181 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA
              241 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT
              301 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA
              361 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA
              421 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG
              481 CGGTCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC
              541 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG
              601 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA
              661 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC
              721 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA
              781 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC
              841 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG
              901 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC
              961 AGGGCACCGG GACAGGTCGG GTCTTGACAA AAAGAACCCG GGGCGCCCCT GCGCTGACAG
             1021 CCGGAACACG CGGGCATCAG AGCAGCGAAT TGTCTGTTTG TGCCCAGTTC ATAGCGGATA
             1081 GCCTCTCCAC CCAAGCGCCG AGACCTGGCG TGCATCAATC TTGTTCAATT CCAATGGCGA
            1141 TAG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.
            reference Tungadi, E.A., Human microcephaly ASPM protein is a spindle pole-focusing factor that functions redundantly with CDK5RAP2. J. Cell Sci. 130 (21): 3676-3684 (2017). PMID 28883092.

            2022.02.09

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