Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK408 (mAID-Nluc Hygro)

A plasmid for construction of C-terminal mAID-Nluc Hygro tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

Distribution information (domestic users) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
付加的提供条件	本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16825_A8Lpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 Sequence file: RDB16825_A8Lpa.seq
>D06157C3_A8Lp_2_pUC-M13_-100_A09_03_ABI24.ab1     1 GGCCCGTCAA GCCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA    61 ACGACGGCCA GTGAGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGA GCTCCCCGGG   121 ATCCGGTGCA GGCGCCAAGG AGAAGAGTGC TTGTCCTAAA GATCCAGCCA AACCTCCGGC   181 CAAGGCACAA GTTGTGGGAT GGCCACCGGT GAGATCATAC CGGAAGAACG TGATGGTTTC   241 CTGCCAAAAA TCAAGCGGTG GCCCGGAGGC GGCGGCGTTC GTGAAGGTAT CAATGGACGG   301 AGCACCGTAC TTGAGGAAAA TCGATTTGAG GATGTATAAA GCTAGCATGG TCTTCACACT   361 CGAAGATTTC GTTGGGGACT GGCGACAGAC AGCCGGCTAC AACCTGGACC AAGTCCTTGA   421 ACAGGGAGGT GTGTCCAGTT TGTTTCAGAA TCTCGGGGTG TCCGTAACTC CGATCCAAAG   481 GATTGTCCTG AGCGGTGAAA ATGGGCTGAA GATCGACATC CATGTCATCA TCCCGTATGA   541 AGGTCTGAGC GGCGACCAAA TGGGCCAGAT CGAAAAAATT TTTAAGGTGG TGTACCCTGT   601 GGATGATCAT CACTTTAAGG TGATCCTGCA CTATGGCACA CTGGTAATCG ACGGGGTTAC   661 GCCGAACATG ATCGACTATT TCGGACGGCC GTATGAAGGC ATCGCCGTGT TCGACGGCAA   721 AAAGATCACT GTAACAGGGA CCCTGTGGAA CGGCAACAAA ATTATCGACG AGCGCCTGAT   781 CAACCCCGAC GGCTCCCTGC TGTTCCGAGT AACCATCAAC GGAGTGACCG GCTGGCGGCT   841 GTGCGAAACG CATTCTGGCG TAAGCGGCCG CTCGAGTCTA GATAACTGAA TCATAATCAG   901 CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCCTTG   961 ACCCTGAAAC ATAAAATGAA TGGCAATTTG GTTGTTGTAA CTTTGTTTAT TGCAGCTTAT  1021 AATGGGTTTA CGAATAAGCA TAGCCATCAC AATTTCACGA ATTAAGGCAT TTTTCCACTG 1081 CATTCTAGTT GTTGGGTTTT GTTTCA //
Primer: reverse Sequence file: RDB16825_A8Lpb.seq
>D06157C3_A8Lp_2_reverse_B09_06_ABI24.ab1     1 CTAAACCAAT TAACCCTCAC TAAAGGGACA AAAGCTGGGT ACCGGATCCT AGTGAACCTC    61 TTCGAGGGAC CTAATAACTT CGTATAGCAT ACATTATACG AAGTTATATT AAGGGTTCCG   121 GATCTCGACC AGCTTCTGAT GGAATTAGAA CTTGGCAAAA CAATACTGAG AATGAAGTGT   181 ATGTGGAACA GAGGCTGCTG ATCTCGTTCT TCAGGCTATG AAACTGACAC ATTTGGAAAC   241 CACAGTACTT AGAACCACAA AGTGGGAATC AAGAGAAAAA CAATGATCCC ACGAGAGATC   301 TATAGATCTA TAGATCATGA GTGGGAGGAA TGAGCTGGCC CTTAATTTGG TTTTGCTTGT   361 TTAAATTATG ATATCCAACT ATGAAACATT ATCATAAAGC AATAGTAAAG AGCCTTCAGT   421 AAAGAGCAGG CATTTATCTA ATCCCACCCC ACCCCCACCC CCGTAGCTCC AATCCTTCCA   481 TTCAAAATGT AGGTACTCTG TTCTCACCCT TCTTAACAAA GTATGACAGG AAAAACTTCC   541 ATTTTAGTGG ACATCTTTAT TGTTTAATAG ATCATCAATT TCTGCATCCC GGGGATCTGA   601 TATCATCGAT GCATGGGGTC GTGCGCTCCT TTCGGTCGGG CGCTGCGGGT CGTGGGGCGG   661 GCGGCTAGCC TATTCCTTTG CCCTCGGACG AGTGCTGGGG CGTCGGTTTC CACTATCGGC   721 GAGTACTTCT ACACAGCCAT CGGTCCAGAC GGCCGCGCTT CTGCGGGCGA TTTGTGTACG   781 CCCGACAGTC CCGGCTCCGG ATCGGACGAT TGCGTCGCAT CGACCCTGCG CCCAAGCTGC   841 ATCATCGAAA TTGCCGTCAA CCAAGCTCTG ATAGAGTTGG TCAAGACCAA TGCGGAGCAT   901 ATACGCCCGG AGCCGCGGCG ATCCTGCAAG CTCCGGATGC CTCCGCTCGA AGTAGCGCGT   961 CTGCTGCTCC ATACAAGCCC AACCACGGGC CTCCAGAGAA GAATGGTGGC GACCTCGTAT  1021 TGGGGGAATC CCCGACATCC GCCCTCGCTT CCAGTCAATG AACCGGCTGA TAATGCGGAC 1081 CAATTGGTTC CGT //
Primer: PGK_N_rev Sequence file: RDB16825_A8Lpc.seq
>D06157C3_A8Lp_2_PGK_N_rev_C09_09_ABI24.ab1     1 CATGAGACGC CTCCTACCCG GTAGATTTCG AGGTCGAGAT CCTAAGCTTG GCTGGACGTA    61 AACTCCTCTT CAGACCTAAT AACTTCGTAT AGCATACATT ATACGAAGTT ATATTAAGGG   121 TTATTGAATA TGATCGACGC GTTAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA   181 ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC   241 ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT   301 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT   361 GATTATGATC AGTTATCTAG ACTCGAGCGG CCGCTTACGC CAGAATGCGT TCGCACAGCC   421 GCCAGCCGGT CACTCCGTTG ATGGTTACTC GGAACAGCAG GGAGCCGTCG GGGTTGATCA   481 GGCGCTCGTC GATAATTTTG TTGCCGTTCC ACAGGGTCCC TGTTACAGTG ATCTTTTTGC   541 CGTCGAACAC GGCGATGCCT TCATACGGCC GTCCGAAATA GTCGATCATG TTCGGCGTAA   601 CCCCGTCGAT TACCAGTGTG CCATAGTGCA GGATCACCTT AAAGTGATGA TCATCCACAG   661 GGTACACCAC CTTAAAAATT TTTTCGATCT GGCCCATTTG GTCGCCGCTC AGACCTTCAT   721 ACGGGATGAT GACATGGATG TCGATCTTCA GCCCATTTTC ACCGCTCAGG ACAATCCTTT   781 GGATCGGAGT TACGGACACC CCGAGATTCT GAAACAAACT GGACACACCT CCCTGTTCAA   841 GGACTTGGTC CAGGTTGTAG CCGGCTGTCT GTCGCCAGTC CCCAACGAAA TCTTCGAGTG   901 TGAAGACCAT GCTAGCTTTA TACATCCTCA AATCGATTTT CCCTCAAGTA CGGTGCTCCG   961 TCCATTGATA CCTTCACGAA CGCCCGCCCG CCTCCCGGCC ACCGCTTTGA TTTTTGGCAG  1021 AAACCCATCA CGTTCGTCAG TATGATCTCA CCGGTGGTCA ATCCCACACC TGTGCCTTGG 1081 ACTGAGTTTT GACTTGGAAT C //
Primer: PGKpro_F Sequence file: RDB16825_A8Lpd.seq
>D06157C3_A8Lp_2_PGKpro_F_D09_12_ABI24.ab1     1 AGTCGGGCCG ATCTCTCTTC TCATCTCCGG GCCTTTCGAC CTGGCATCCA TCTAGATCTC    61 GAGCAGCTGA AGCTTACCGC TAGCATGGAT AGATCCGGAA AGCCTGAACT CACCGCGACG   121 TCTGTCGAGA AGTTTCTGAT CGAAAAGTTC GACAGCGTCT CCGACCTGAT GCAGCTCTCG   181 GAGGGCGAAG AATCTCGTGC TTTCAGCTTC GATGTAGGAG GGCGTGGATA TGTCCTGCGG   241 GTAAATAGCT GCGCCGATGG TTTCTACAAA GATCGTTATG TTTATCGGCA CTTTGCATCG   301 GCCGCGCTCC CGATTCCGGA AGTGCTTGAC ATTGGGGAAT TCAGCGAGAG CCTGACCTAT   361 TGCATCTCCC GCCGTGCACA GGGTGTCACG TTGCAAGACC TGCCTGAAAC CGAACTGCCC   421 GCTGTTCTGC AGCCGGTCGC GGAGGCCATG GATGCGATCG CTGCGGCCGA TCTTAGCCAG   481 ACGAGCGGGT TCGGCCCATT CGGACCGCAA GGAATCGGTC AATACACTAC ATGGCGTGAT   541 TTCATATGCG CGATTGCTGA TCCCCATGTG TATCACTGGC AAACTGTGAT GGACGACACC   601 GTCAGTGCGT CCGTCGCGCA GGCTCTCGAT GAGCTGATGC TTTGGGCCGA GGACTGCCCC   661 GAAGTCCGGC ACCTCGTGCA CGCGGATTTC GGCTCCAACA ATGTCCTGAC GGACAATGGC   721 CGCATAACAG CGGTCATTGA CTGGAGCGAG GCGATGTTCG GGGATTCCCA ATACGAGGTC   781 GCCAACATCT TCTTCTGGAG GCCGTGGTTG GCTTGTATGG AGCAGCAGAC GCGCTACTTC   841 GAGCGGAGGC ATCCGGAGCT TGCAGATCGC CGCGGCTCCG GGCGTATATG CTCCGCATTG   901 GTCTTGACCA ACTCTATCAG AGCTTGGTTT GACGGCAATT TCGATGATGC AGCTTGGGCG   961 CAGGTCGATG CGACGCAATC GTCCCGAATC CGGAGCCCGG GACTGTCGGG CGTACACAAT  1021 CGCCGCAGAG CGCGGATCGT CTGACGATGG CTGTGTAGAG TTACTCGTCG ATAGTTGAAC 1081 CGAACGCCCC CCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2021.11.02

GNP_filter3_RDBDEP_html_211029.pl