Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK408 (mAID-Nluc Hygro)

A plasmid for construction of C-terminal mAID-Nluc Hygro tagging donors.

Catalog number RDB16825
Resource name pMK408 (mAID-Nluc Hygro)
Clone info. A plasmid for construction of C-terminal mAID-Nluc Hygro tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Oplophorus gracilirostris luciferase cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank

External Database
Arabidopsis thaliana AtIAA17 Oplophorus gracilirostris luciferase

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Distribution information (domestic users) [open/close]


          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16825 pMK408 (mAID-Nluc Hygro) DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16825_A8Lpp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pUC-M13_-100
          Sequence file: RDB16825_A8Lpa.seq check
          >D06157C3_A8Lp_2_pUC-M13_-100_A09_03_ABI24.ab1
              1 GGCCCGTCAA GCCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA
             61 ACGACGGCCA GTGAGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGA GCTCCCCGGG
            121 ATCCGGTGCA GGCGCCAAGG AGAAGAGTGC TTGTCCTAAA GATCCAGCCA AACCTCCGGC
            181 CAAGGCACAA GTTGTGGGAT GGCCACCGGT GAGATCATAC CGGAAGAACG TGATGGTTTC
            241 CTGCCAAAAA TCAAGCGGTG GCCCGGAGGC GGCGGCGTTC GTGAAGGTAT CAATGGACGG
            301 AGCACCGTAC TTGAGGAAAA TCGATTTGAG GATGTATAAA GCTAGCATGG TCTTCACACT
            361 CGAAGATTTC GTTGGGGACT GGCGACAGAC AGCCGGCTAC AACCTGGACC AAGTCCTTGA
            421 ACAGGGAGGT GTGTCCAGTT TGTTTCAGAA TCTCGGGGTG TCCGTAACTC CGATCCAAAG
            481 GATTGTCCTG AGCGGTGAAA ATGGGCTGAA GATCGACATC CATGTCATCA TCCCGTATGA
            541 AGGTCTGAGC GGCGACCAAA TGGGCCAGAT CGAAAAAATT TTTAAGGTGG TGTACCCTGT
            601 GGATGATCAT CACTTTAAGG TGATCCTGCA CTATGGCACA CTGGTAATCG ACGGGGTTAC
            661 GCCGAACATG ATCGACTATT TCGGACGGCC GTATGAAGGC ATCGCCGTGT TCGACGGCAA
            721 AAAGATCACT GTAACAGGGA CCCTGTGGAA CGGCAACAAA ATTATCGACG AGCGCCTGAT
            781 CAACCCCGAC GGCTCCCTGC TGTTCCGAGT AACCATCAAC GGAGTGACCG GCTGGCGGCT
            841 GTGCGAAACG CATTCTGGCG TAAGCGGCCG CTCGAGTCTA GATAACTGAA TCATAATCAG
            901 CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCCTTG
            961 ACCCTGAAAC ATAAAATGAA TGGCAATTTG GTTGTTGTAA CTTTGTTTAT TGCAGCTTAT
           1021 AATGGGTTTA CGAATAAGCA TAGCCATCAC AATTTCACGA ATTAAGGCAT TTTTCCACTG
          1081 CATTCTAGTT GTTGGGTTTT GTTTCA
          //
          Primer: reverse
          Sequence file: RDB16825_A8Lpb.seq check
          >D06157C3_A8Lp_2_reverse_B09_06_ABI24.ab1
              1 CTAAACCAAT TAACCCTCAC TAAAGGGACA AAAGCTGGGT ACCGGATCCT AGTGAACCTC
             61 TTCGAGGGAC CTAATAACTT CGTATAGCAT ACATTATACG AAGTTATATT AAGGGTTCCG
            121 GATCTCGACC AGCTTCTGAT GGAATTAGAA CTTGGCAAAA CAATACTGAG AATGAAGTGT
            181 ATGTGGAACA GAGGCTGCTG ATCTCGTTCT TCAGGCTATG AAACTGACAC ATTTGGAAAC
            241 CACAGTACTT AGAACCACAA AGTGGGAATC AAGAGAAAAA CAATGATCCC ACGAGAGATC
            301 TATAGATCTA TAGATCATGA GTGGGAGGAA TGAGCTGGCC CTTAATTTGG TTTTGCTTGT
            361 TTAAATTATG ATATCCAACT ATGAAACATT ATCATAAAGC AATAGTAAAG AGCCTTCAGT
            421 AAAGAGCAGG CATTTATCTA ATCCCACCCC ACCCCCACCC CCGTAGCTCC AATCCTTCCA
            481 TTCAAAATGT AGGTACTCTG TTCTCACCCT TCTTAACAAA GTATGACAGG AAAAACTTCC
            541 ATTTTAGTGG ACATCTTTAT TGTTTAATAG ATCATCAATT TCTGCATCCC GGGGATCTGA
            601 TATCATCGAT GCATGGGGTC GTGCGCTCCT TTCGGTCGGG CGCTGCGGGT CGTGGGGCGG
            661 GCGGCTAGCC TATTCCTTTG CCCTCGGACG AGTGCTGGGG CGTCGGTTTC CACTATCGGC
            721 GAGTACTTCT ACACAGCCAT CGGTCCAGAC GGCCGCGCTT CTGCGGGCGA TTTGTGTACG
            781 CCCGACAGTC CCGGCTCCGG ATCGGACGAT TGCGTCGCAT CGACCCTGCG CCCAAGCTGC
            841 ATCATCGAAA TTGCCGTCAA CCAAGCTCTG ATAGAGTTGG TCAAGACCAA TGCGGAGCAT
            901 ATACGCCCGG AGCCGCGGCG ATCCTGCAAG CTCCGGATGC CTCCGCTCGA AGTAGCGCGT
            961 CTGCTGCTCC ATACAAGCCC AACCACGGGC CTCCAGAGAA GAATGGTGGC GACCTCGTAT
           1021 TGGGGGAATC CCCGACATCC GCCCTCGCTT CCAGTCAATG AACCGGCTGA TAATGCGGAC
          1081 CAATTGGTTC CGT
          //
          Primer: PGK_N_rev
          Sequence file: RDB16825_A8Lpc.seq check
          >D06157C3_A8Lp_2_PGK_N_rev_C09_09_ABI24.ab1
              1 CATGAGACGC CTCCTACCCG GTAGATTTCG AGGTCGAGAT CCTAAGCTTG GCTGGACGTA
             61 AACTCCTCTT CAGACCTAAT AACTTCGTAT AGCATACATT ATACGAAGTT ATATTAAGGG
            121 TTATTGAATA TGATCGACGC GTTAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA
            181 ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC
            241 ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT
            301 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
            361 GATTATGATC AGTTATCTAG ACTCGAGCGG CCGCTTACGC CAGAATGCGT TCGCACAGCC
            421 GCCAGCCGGT CACTCCGTTG ATGGTTACTC GGAACAGCAG GGAGCCGTCG GGGTTGATCA
            481 GGCGCTCGTC GATAATTTTG TTGCCGTTCC ACAGGGTCCC TGTTACAGTG ATCTTTTTGC
            541 CGTCGAACAC GGCGATGCCT TCATACGGCC GTCCGAAATA GTCGATCATG TTCGGCGTAA
            601 CCCCGTCGAT TACCAGTGTG CCATAGTGCA GGATCACCTT AAAGTGATGA TCATCCACAG
            661 GGTACACCAC CTTAAAAATT TTTTCGATCT GGCCCATTTG GTCGCCGCTC AGACCTTCAT
            721 ACGGGATGAT GACATGGATG TCGATCTTCA GCCCATTTTC ACCGCTCAGG ACAATCCTTT
            781 GGATCGGAGT TACGGACACC CCGAGATTCT GAAACAAACT GGACACACCT CCCTGTTCAA
            841 GGACTTGGTC CAGGTTGTAG CCGGCTGTCT GTCGCCAGTC CCCAACGAAA TCTTCGAGTG
            901 TGAAGACCAT GCTAGCTTTA TACATCCTCA AATCGATTTT CCCTCAAGTA CGGTGCTCCG
            961 TCCATTGATA CCTTCACGAA CGCCCGCCCG CCTCCCGGCC ACCGCTTTGA TTTTTGGCAG
           1021 AAACCCATCA CGTTCGTCAG TATGATCTCA CCGGTGGTCA ATCCCACACC TGTGCCTTGG
          1081 ACTGAGTTTT GACTTGGAAT C
          //
          Primer: PGKpro_F
          Sequence file: RDB16825_A8Lpd.seq check
          >D06157C3_A8Lp_2_PGKpro_F_D09_12_ABI24.ab1
              1 AGTCGGGCCG ATCTCTCTTC TCATCTCCGG GCCTTTCGAC CTGGCATCCA TCTAGATCTC
             61 GAGCAGCTGA AGCTTACCGC TAGCATGGAT AGATCCGGAA AGCCTGAACT CACCGCGACG
            121 TCTGTCGAGA AGTTTCTGAT CGAAAAGTTC GACAGCGTCT CCGACCTGAT GCAGCTCTCG
            181 GAGGGCGAAG AATCTCGTGC TTTCAGCTTC GATGTAGGAG GGCGTGGATA TGTCCTGCGG
            241 GTAAATAGCT GCGCCGATGG TTTCTACAAA GATCGTTATG TTTATCGGCA CTTTGCATCG
            301 GCCGCGCTCC CGATTCCGGA AGTGCTTGAC ATTGGGGAAT TCAGCGAGAG CCTGACCTAT
            361 TGCATCTCCC GCCGTGCACA GGGTGTCACG TTGCAAGACC TGCCTGAAAC CGAACTGCCC
            421 GCTGTTCTGC AGCCGGTCGC GGAGGCCATG GATGCGATCG CTGCGGCCGA TCTTAGCCAG
            481 ACGAGCGGGT TCGGCCCATT CGGACCGCAA GGAATCGGTC AATACACTAC ATGGCGTGAT
            541 TTCATATGCG CGATTGCTGA TCCCCATGTG TATCACTGGC AAACTGTGAT GGACGACACC
            601 GTCAGTGCGT CCGTCGCGCA GGCTCTCGAT GAGCTGATGC TTTGGGCCGA GGACTGCCCC
            661 GAAGTCCGGC ACCTCGTGCA CGCGGATTTC GGCTCCAACA ATGTCCTGAC GGACAATGGC
            721 CGCATAACAG CGGTCATTGA CTGGAGCGAG GCGATGTTCG GGGATTCCCA ATACGAGGTC
            781 GCCAACATCT TCTTCTGGAG GCCGTGGTTG GCTTGTATGG AGCAGCAGAC GCGCTACTTC
            841 GAGCGGAGGC ATCCGGAGCT TGCAGATCGC CGCGGCTCCG GGCGTATATG CTCCGCATTG
            901 GTCTTGACCA ACTCTATCAG AGCTTGGTTT GACGGCAATT TCGATGATGC AGCTTGGGCG
            961 CAGGTCGATG CGACGCAATC GTCCCGAATC CGGAGCCCGG GACTGTCGGG CGTACACAAT
           1021 CGCCGCAGAG CGCGGATCGT CTGACGATGG CTGTGTAGAG TTACTCGTCG ATAGTTGAAC
          1081 CGAACGCCCC CCA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

          2021.11.02

          GNP_filter3_RDBDEP_html_211029.pl