Sequencing and PCR primers for primer information.
A plasmid for construction of C-terminal mAID-Nluc Hygro tagging donors.
| Catalog number | RDB16825 |
|---|---|
| Resource name | pMK408 (mAID-Nluc Hygro) |
| Clone info. | A plasmid for construction of C-terminal mAID-Nluc Hygro tagging donors. |
| Vector backbone | pBluescript (plasmid) |
| Size of vector backbone | 2.9 kb |
| Selectable markers | Am^r (E. coli) |
| Gene/insert name | Arabidopsis thaliana AtIAA17 cDNA Oplophorus gracilirostris luciferase cDNA |
| Depositor | Kanemaki, Masato | |
| Other clones in our bank | Arabidopsis thaliana AXR3 (KEGG orthology K14484) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
|---|---|
| Additional terms and conditions for distribution | The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation. |
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB16825 | pMK408 (mAID-Nluc Hygro) | DNA solution |
Electronic file
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Auxin Inducible Degron (AID) System (English text) |
|---|---|
| Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB16825_A8Lpp1.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
| Primer: pUC-M13_-100 | RDB16825_A8Lpa.seq |
|---|---|
|
>D06157C3_A8Lp_2_pUC-M13_-100_A09_03_ABI24.ab1 1 GGCCCGTCAA GCCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA 61 ACGACGGCCA GTGAGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGA GCTCCCCGGG 121 ATCCGGTGCA GGCGCCAAGG AGAAGAGTGC TTGTCCTAAA GATCCAGCCA AACCTCCGGC 181 CAAGGCACAA GTTGTGGGAT GGCCACCGGT GAGATCATAC CGGAAGAACG TGATGGTTTC 241 CTGCCAAAAA TCAAGCGGTG GCCCGGAGGC GGCGGCGTTC GTGAAGGTAT CAATGGACGG 301 AGCACCGTAC TTGAGGAAAA TCGATTTGAG GATGTATAAA GCTAGCATGG TCTTCACACT 361 CGAAGATTTC GTTGGGGACT GGCGACAGAC AGCCGGCTAC AACCTGGACC AAGTCCTTGA 421 ACAGGGAGGT GTGTCCAGTT TGTTTCAGAA TCTCGGGGTG TCCGTAACTC CGATCCAAAG 481 GATTGTCCTG AGCGGTGAAA ATGGGCTGAA GATCGACATC CATGTCATCA TCCCGTATGA 541 AGGTCTGAGC GGCGACCAAA TGGGCCAGAT CGAAAAAATT TTTAAGGTGG TGTACCCTGT 601 GGATGATCAT CACTTTAAGG TGATCCTGCA CTATGGCACA CTGGTAATCG ACGGGGTTAC 661 GCCGAACATG ATCGACTATT TCGGACGGCC GTATGAAGGC ATCGCCGTGT TCGACGGCAA 721 AAAGATCACT GTAACAGGGA CCCTGTGGAA CGGCAACAAA ATTATCGACG AGCGCCTGAT 781 CAACCCCGAC GGCTCCCTGC TGTTCCGAGT AACCATCAAC GGAGTGACCG GCTGGCGGCT 841 GTGCGAAACG CATTCTGGCG TAAGCGGCCG CTCGAGTCTA GATAACTGAA TCATAATCAG 901 CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCCTTG 961 ACCCTGAAAC ATAAAATGAA TGGCAATTTG GTTGTTGTAA CTTTGTTTAT TGCAGCTTAT 1021 AATGGGTTTA CGAATAAGCA TAGCCATCAC AATTTCACGA ATTAAGGCAT TTTTCCACTG 1081 CATTCTAGTT GTTGGGTTTT GTTTCA // |
|
| Primer: reverse | RDB16825_A8Lpb.seq |
|
>D06157C3_A8Lp_2_reverse_B09_06_ABI24.ab1 1 CTAAACCAAT TAACCCTCAC TAAAGGGACA AAAGCTGGGT ACCGGATCCT AGTGAACCTC 61 TTCGAGGGAC CTAATAACTT CGTATAGCAT ACATTATACG AAGTTATATT AAGGGTTCCG 121 GATCTCGACC AGCTTCTGAT GGAATTAGAA CTTGGCAAAA CAATACTGAG AATGAAGTGT 181 ATGTGGAACA GAGGCTGCTG ATCTCGTTCT TCAGGCTATG AAACTGACAC ATTTGGAAAC 241 CACAGTACTT AGAACCACAA AGTGGGAATC AAGAGAAAAA CAATGATCCC ACGAGAGATC 301 TATAGATCTA TAGATCATGA GTGGGAGGAA TGAGCTGGCC CTTAATTTGG TTTTGCTTGT 361 TTAAATTATG ATATCCAACT ATGAAACATT ATCATAAAGC AATAGTAAAG AGCCTTCAGT 421 AAAGAGCAGG CATTTATCTA ATCCCACCCC ACCCCCACCC CCGTAGCTCC AATCCTTCCA 481 TTCAAAATGT AGGTACTCTG TTCTCACCCT TCTTAACAAA GTATGACAGG AAAAACTTCC 541 ATTTTAGTGG ACATCTTTAT TGTTTAATAG ATCATCAATT TCTGCATCCC GGGGATCTGA 601 TATCATCGAT GCATGGGGTC GTGCGCTCCT TTCGGTCGGG CGCTGCGGGT CGTGGGGCGG 661 GCGGCTAGCC TATTCCTTTG CCCTCGGACG AGTGCTGGGG CGTCGGTTTC CACTATCGGC 721 GAGTACTTCT ACACAGCCAT CGGTCCAGAC GGCCGCGCTT CTGCGGGCGA TTTGTGTACG 781 CCCGACAGTC CCGGCTCCGG ATCGGACGAT TGCGTCGCAT CGACCCTGCG CCCAAGCTGC 841 ATCATCGAAA TTGCCGTCAA CCAAGCTCTG ATAGAGTTGG TCAAGACCAA TGCGGAGCAT 901 ATACGCCCGG AGCCGCGGCG ATCCTGCAAG CTCCGGATGC CTCCGCTCGA AGTAGCGCGT 961 CTGCTGCTCC ATACAAGCCC AACCACGGGC CTCCAGAGAA GAATGGTGGC GACCTCGTAT 1021 TGGGGGAATC CCCGACATCC GCCCTCGCTT CCAGTCAATG AACCGGCTGA TAATGCGGAC 1081 CAATTGGTTC CGT // |
|
| Primer: PGK_N_rev | RDB16825_A8Lpc.seq |
|
>D06157C3_A8Lp_2_PGK_N_rev_C09_09_ABI24.ab1 1 CATGAGACGC CTCCTACCCG GTAGATTTCG AGGTCGAGAT CCTAAGCTTG GCTGGACGTA 61 AACTCCTCTT CAGACCTAAT AACTTCGTAT AGCATACATT ATACGAAGTT ATATTAAGGG 121 TTATTGAATA TGATCGACGC GTTAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA 181 ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC 241 ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT 301 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT 361 GATTATGATC AGTTATCTAG ACTCGAGCGG CCGCTTACGC CAGAATGCGT TCGCACAGCC 421 GCCAGCCGGT CACTCCGTTG ATGGTTACTC GGAACAGCAG GGAGCCGTCG GGGTTGATCA 481 GGCGCTCGTC GATAATTTTG TTGCCGTTCC ACAGGGTCCC TGTTACAGTG ATCTTTTTGC 541 CGTCGAACAC GGCGATGCCT TCATACGGCC GTCCGAAATA GTCGATCATG TTCGGCGTAA 601 CCCCGTCGAT TACCAGTGTG CCATAGTGCA GGATCACCTT AAAGTGATGA TCATCCACAG 661 GGTACACCAC CTTAAAAATT TTTTCGATCT GGCCCATTTG GTCGCCGCTC AGACCTTCAT 721 ACGGGATGAT GACATGGATG TCGATCTTCA GCCCATTTTC ACCGCTCAGG ACAATCCTTT 781 GGATCGGAGT TACGGACACC CCGAGATTCT GAAACAAACT GGACACACCT CCCTGTTCAA 841 GGACTTGGTC CAGGTTGTAG CCGGCTGTCT GTCGCCAGTC CCCAACGAAA TCTTCGAGTG 901 TGAAGACCAT GCTAGCTTTA TACATCCTCA AATCGATTTT CCCTCAAGTA CGGTGCTCCG 961 TCCATTGATA CCTTCACGAA CGCCCGCCCG CCTCCCGGCC ACCGCTTTGA TTTTTGGCAG 1021 AAACCCATCA CGTTCGTCAG TATGATCTCA CCGGTGGTCA ATCCCACACC TGTGCCTTGG 1081 ACTGAGTTTT GACTTGGAAT C // |
|
| Primer: PGKpro_F | RDB16825_A8Lpd.seq |
|
>D06157C3_A8Lp_2_PGKpro_F_D09_12_ABI24.ab1 1 AGTCGGGCCG ATCTCTCTTC TCATCTCCGG GCCTTTCGAC CTGGCATCCA TCTAGATCTC 61 GAGCAGCTGA AGCTTACCGC TAGCATGGAT AGATCCGGAA AGCCTGAACT CACCGCGACG 121 TCTGTCGAGA AGTTTCTGAT CGAAAAGTTC GACAGCGTCT CCGACCTGAT GCAGCTCTCG 181 GAGGGCGAAG AATCTCGTGC TTTCAGCTTC GATGTAGGAG GGCGTGGATA TGTCCTGCGG 241 GTAAATAGCT GCGCCGATGG TTTCTACAAA GATCGTTATG TTTATCGGCA CTTTGCATCG 301 GCCGCGCTCC CGATTCCGGA AGTGCTTGAC ATTGGGGAAT TCAGCGAGAG CCTGACCTAT 361 TGCATCTCCC GCCGTGCACA GGGTGTCACG TTGCAAGACC TGCCTGAAAC CGAACTGCCC 421 GCTGTTCTGC AGCCGGTCGC GGAGGCCATG GATGCGATCG CTGCGGCCGA TCTTAGCCAG 481 ACGAGCGGGT TCGGCCCATT CGGACCGCAA GGAATCGGTC AATACACTAC ATGGCGTGAT 541 TTCATATGCG CGATTGCTGA TCCCCATGTG TATCACTGGC AAACTGTGAT GGACGACACC 601 GTCAGTGCGT CCGTCGCGCA GGCTCTCGAT GAGCTGATGC TTTGGGCCGA GGACTGCCCC 661 GAAGTCCGGC ACCTCGTGCA CGCGGATTTC GGCTCCAACA ATGTCCTGAC GGACAATGGC 721 CGCATAACAG CGGTCATTGA CTGGAGCGAG GCGATGTTCG GGGATTCCCA ATACGAGGTC 781 GCCAACATCT TCTTCTGGAG GCCGTGGTTG GCTTGTATGG AGCAGCAGAC GCGCTACTTC 841 GAGCGGAGGC ATCCGGAGCT TGCAGATCGC CGCGGCTCCG GGCGTATATG CTCCGCATTG 901 GTCTTGACCA ACTCTATCAG AGCTTGGTTT GACGGCAATT TCGATGATGC AGCTTGGGCG 961 CAGGTCGATG CGACGCAATC GTCCCGAATC CGGAGCCCGG GACTGTCGGG CGTACACAAT 1021 CGCCGCAGAG CGCGGATCGT CTGACGATGG CTGTGTAGAG TTACTCGTCG ATAGTTGAAC 1081 CGAACGCCCC CCA // |
|
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
2019.07.12
GNP_filter3_RDBDEP_html_190431.pl