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pMK408 (mAID-Nluc Hygro)

A plasmid for construction of C-terminal mAID-Nluc Hygro tagging donors.

Catalog number RDB16825
Resource name pMK408 (mAID-Nluc Hygro)
Clone info. A plasmid for construction of C-terminal mAID-Nluc Hygro tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Oplophorus gracilirostris luciferase cDNA
Depositor Kanemaki, Masato |
 
Other clones in our bank Arabidopsis thaliana AXR3 (KEGG orthology K14484) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16825 pMK408 (mAID-Nluc Hygro) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16825_A8Lpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 RDB16825_A8Lpa.seq
>D06157C3_A8Lp_2_pUC-M13_-100_A09_03_ABI24.ab1
    1 GGCCCGTCAA GCCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA
   61 ACGACGGCCA GTGAGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGA GCTCCCCGGG
  121 ATCCGGTGCA GGCGCCAAGG AGAAGAGTGC TTGTCCTAAA GATCCAGCCA AACCTCCGGC
  181 CAAGGCACAA GTTGTGGGAT GGCCACCGGT GAGATCATAC CGGAAGAACG TGATGGTTTC
  241 CTGCCAAAAA TCAAGCGGTG GCCCGGAGGC GGCGGCGTTC GTGAAGGTAT CAATGGACGG
  301 AGCACCGTAC TTGAGGAAAA TCGATTTGAG GATGTATAAA GCTAGCATGG TCTTCACACT
  361 CGAAGATTTC GTTGGGGACT GGCGACAGAC AGCCGGCTAC AACCTGGACC AAGTCCTTGA
  421 ACAGGGAGGT GTGTCCAGTT TGTTTCAGAA TCTCGGGGTG TCCGTAACTC CGATCCAAAG
  481 GATTGTCCTG AGCGGTGAAA ATGGGCTGAA GATCGACATC CATGTCATCA TCCCGTATGA
  541 AGGTCTGAGC GGCGACCAAA TGGGCCAGAT CGAAAAAATT TTTAAGGTGG TGTACCCTGT
  601 GGATGATCAT CACTTTAAGG TGATCCTGCA CTATGGCACA CTGGTAATCG ACGGGGTTAC
  661 GCCGAACATG ATCGACTATT TCGGACGGCC GTATGAAGGC ATCGCCGTGT TCGACGGCAA
  721 AAAGATCACT GTAACAGGGA CCCTGTGGAA CGGCAACAAA ATTATCGACG AGCGCCTGAT
  781 CAACCCCGAC GGCTCCCTGC TGTTCCGAGT AACCATCAAC GGAGTGACCG GCTGGCGGCT
  841 GTGCGAAACG CATTCTGGCG TAAGCGGCCG CTCGAGTCTA GATAACTGAA TCATAATCAG
  901 CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCCTTG
  961 ACCCTGAAAC ATAAAATGAA TGGCAATTTG GTTGTTGTAA CTTTGTTTAT TGCAGCTTAT
 1021 AATGGGTTTA CGAATAAGCA TAGCCATCAC AATTTCACGA ATTAAGGCAT TTTTCCACTG
1081 CATTCTAGTT GTTGGGTTTT GTTTCA
//
Primer: reverse RDB16825_A8Lpb.seq
>D06157C3_A8Lp_2_reverse_B09_06_ABI24.ab1
    1 CTAAACCAAT TAACCCTCAC TAAAGGGACA AAAGCTGGGT ACCGGATCCT AGTGAACCTC
   61 TTCGAGGGAC CTAATAACTT CGTATAGCAT ACATTATACG AAGTTATATT AAGGGTTCCG
  121 GATCTCGACC AGCTTCTGAT GGAATTAGAA CTTGGCAAAA CAATACTGAG AATGAAGTGT
  181 ATGTGGAACA GAGGCTGCTG ATCTCGTTCT TCAGGCTATG AAACTGACAC ATTTGGAAAC
  241 CACAGTACTT AGAACCACAA AGTGGGAATC AAGAGAAAAA CAATGATCCC ACGAGAGATC
  301 TATAGATCTA TAGATCATGA GTGGGAGGAA TGAGCTGGCC CTTAATTTGG TTTTGCTTGT
  361 TTAAATTATG ATATCCAACT ATGAAACATT ATCATAAAGC AATAGTAAAG AGCCTTCAGT
  421 AAAGAGCAGG CATTTATCTA ATCCCACCCC ACCCCCACCC CCGTAGCTCC AATCCTTCCA
  481 TTCAAAATGT AGGTACTCTG TTCTCACCCT TCTTAACAAA GTATGACAGG AAAAACTTCC
  541 ATTTTAGTGG ACATCTTTAT TGTTTAATAG ATCATCAATT TCTGCATCCC GGGGATCTGA
  601 TATCATCGAT GCATGGGGTC GTGCGCTCCT TTCGGTCGGG CGCTGCGGGT CGTGGGGCGG
  661 GCGGCTAGCC TATTCCTTTG CCCTCGGACG AGTGCTGGGG CGTCGGTTTC CACTATCGGC
  721 GAGTACTTCT ACACAGCCAT CGGTCCAGAC GGCCGCGCTT CTGCGGGCGA TTTGTGTACG
  781 CCCGACAGTC CCGGCTCCGG ATCGGACGAT TGCGTCGCAT CGACCCTGCG CCCAAGCTGC
  841 ATCATCGAAA TTGCCGTCAA CCAAGCTCTG ATAGAGTTGG TCAAGACCAA TGCGGAGCAT
  901 ATACGCCCGG AGCCGCGGCG ATCCTGCAAG CTCCGGATGC CTCCGCTCGA AGTAGCGCGT
  961 CTGCTGCTCC ATACAAGCCC AACCACGGGC CTCCAGAGAA GAATGGTGGC GACCTCGTAT
 1021 TGGGGGAATC CCCGACATCC GCCCTCGCTT CCAGTCAATG AACCGGCTGA TAATGCGGAC
1081 CAATTGGTTC CGT
//
Primer: PGK_N_rev RDB16825_A8Lpc.seq
>D06157C3_A8Lp_2_PGK_N_rev_C09_09_ABI24.ab1
    1 CATGAGACGC CTCCTACCCG GTAGATTTCG AGGTCGAGAT CCTAAGCTTG GCTGGACGTA
   61 AACTCCTCTT CAGACCTAAT AACTTCGTAT AGCATACATT ATACGAAGTT ATATTAAGGG
  121 TTATTGAATA TGATCGACGC GTTAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA
  181 ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC
  241 ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT
  301 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
  361 GATTATGATC AGTTATCTAG ACTCGAGCGG CCGCTTACGC CAGAATGCGT TCGCACAGCC
  421 GCCAGCCGGT CACTCCGTTG ATGGTTACTC GGAACAGCAG GGAGCCGTCG GGGTTGATCA
  481 GGCGCTCGTC GATAATTTTG TTGCCGTTCC ACAGGGTCCC TGTTACAGTG ATCTTTTTGC
  541 CGTCGAACAC GGCGATGCCT TCATACGGCC GTCCGAAATA GTCGATCATG TTCGGCGTAA
  601 CCCCGTCGAT TACCAGTGTG CCATAGTGCA GGATCACCTT AAAGTGATGA TCATCCACAG
  661 GGTACACCAC CTTAAAAATT TTTTCGATCT GGCCCATTTG GTCGCCGCTC AGACCTTCAT
  721 ACGGGATGAT GACATGGATG TCGATCTTCA GCCCATTTTC ACCGCTCAGG ACAATCCTTT
  781 GGATCGGAGT TACGGACACC CCGAGATTCT GAAACAAACT GGACACACCT CCCTGTTCAA
  841 GGACTTGGTC CAGGTTGTAG CCGGCTGTCT GTCGCCAGTC CCCAACGAAA TCTTCGAGTG
  901 TGAAGACCAT GCTAGCTTTA TACATCCTCA AATCGATTTT CCCTCAAGTA CGGTGCTCCG
  961 TCCATTGATA CCTTCACGAA CGCCCGCCCG CCTCCCGGCC ACCGCTTTGA TTTTTGGCAG
 1021 AAACCCATCA CGTTCGTCAG TATGATCTCA CCGGTGGTCA ATCCCACACC TGTGCCTTGG
1081 ACTGAGTTTT GACTTGGAAT C
//
Primer: PGKpro_F RDB16825_A8Lpd.seq
>D06157C3_A8Lp_2_PGKpro_F_D09_12_ABI24.ab1
    1 AGTCGGGCCG ATCTCTCTTC TCATCTCCGG GCCTTTCGAC CTGGCATCCA TCTAGATCTC
   61 GAGCAGCTGA AGCTTACCGC TAGCATGGAT AGATCCGGAA AGCCTGAACT CACCGCGACG
  121 TCTGTCGAGA AGTTTCTGAT CGAAAAGTTC GACAGCGTCT CCGACCTGAT GCAGCTCTCG
  181 GAGGGCGAAG AATCTCGTGC TTTCAGCTTC GATGTAGGAG GGCGTGGATA TGTCCTGCGG
  241 GTAAATAGCT GCGCCGATGG TTTCTACAAA GATCGTTATG TTTATCGGCA CTTTGCATCG
  301 GCCGCGCTCC CGATTCCGGA AGTGCTTGAC ATTGGGGAAT TCAGCGAGAG CCTGACCTAT
  361 TGCATCTCCC GCCGTGCACA GGGTGTCACG TTGCAAGACC TGCCTGAAAC CGAACTGCCC
  421 GCTGTTCTGC AGCCGGTCGC GGAGGCCATG GATGCGATCG CTGCGGCCGA TCTTAGCCAG
  481 ACGAGCGGGT TCGGCCCATT CGGACCGCAA GGAATCGGTC AATACACTAC ATGGCGTGAT
  541 TTCATATGCG CGATTGCTGA TCCCCATGTG TATCACTGGC AAACTGTGAT GGACGACACC
  601 GTCAGTGCGT CCGTCGCGCA GGCTCTCGAT GAGCTGATGC TTTGGGCCGA GGACTGCCCC
  661 GAAGTCCGGC ACCTCGTGCA CGCGGATTTC GGCTCCAACA ATGTCCTGAC GGACAATGGC
  721 CGCATAACAG CGGTCATTGA CTGGAGCGAG GCGATGTTCG GGGATTCCCA ATACGAGGTC
  781 GCCAACATCT TCTTCTGGAG GCCGTGGTTG GCTTGTATGG AGCAGCAGAC GCGCTACTTC
  841 GAGCGGAGGC ATCCGGAGCT TGCAGATCGC CGCGGCTCCG GGCGTATATG CTCCGCATTG
  901 GTCTTGACCA ACTCTATCAG AGCTTGGTTT GACGGCAATT TCGATGATGC AGCTTGGGCG
  961 CAGGTCGATG CGACGCAATC GTCCCGAATC CGGAGCCCGG GACTGTCGGG CGTACACAAT
 1021 CGCCGCAGAG CGCGGATCGT CTGACGATGG CTGTGTAGAG TTACTCGTCG ATAGTTGAAC
1081 CGAACGCCCC CCA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

2019.07.12

GNP_filter3_RDBDEP_html_190431.pl