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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAY7

TOL2 expression vector of OsTIR1(WT) and mAID-EGFP-NLS marker.

Catalog number RDB18333
Resource name pAY7
Alternative name OsTIR1 mAID-EGFP-NLS TOL2
Clone info. TOL2 expression vector of OsTIR1(WT) and mAID-EGFP-NLS marker, see Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone pT2AL200R175-CAGGS-EGFP (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell).
Gene/insert name Oryza sativa OsTIR1 cDNA Arabidopsis thaliana IAA17 cDNA Aequorea victoria GFP (EGFP) cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank

External Database
Oryza sativa OsTIR1 Arabidopsis thaliana IAA17 Aequorea victoria GFP (EGFP)

        Reference sequence
          
         
        Sequence (full) RDB18333zzk01.seq provided by the depositor

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
        Additional terms and conditions for distribution The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Koichi Kawakami of the National Institute of Genetics (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004))is requested.
        Ordering Please visit Information of Request for Distribution.[link] 
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        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
        提供案内 (日本国内) [open/close]

        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
        付加的提供条件 Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。利用者は、研究成果の公表にあたって国立遺伝学研究所 川上浩一博士が指定する文献 (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004)) の引用を必要とする。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB18333 pAY7 DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Original reference

        original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Auxin Inducible Degron (AID) System (English text)
        Featured content Auxin Inducible Degron (AID) System (Japanese text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB18333_B0Gfp1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: pAxCA_F1
        Region: OsTIR1 (WT) 5'
        Sequence file: RDB18333_B0Gfa.seq check
        >18333_18333_B0Gf_3_pAxCA_F1_A03_03_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNTTCN NNNNNNTCCT GGGCAACGNN
           61 NNNGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCC TCGAAGCGCT GCCACCATGA
          121 CATACTTTCC TGAAGAGGTC GTCGAACACA TTTTTAGCTT CCTGCCTGCA CAGAGAGATA
          181 GAAACACAGT GAGCCTGGTC TGCAAAGTGT GGTACGAGAT CGAACGCCTG AGCCGGAGAG
          241 GAGTGTTCGT CGGCAACTGC TATGCTGTGA GAGCAGGCAG GGTCGCCGCT AGGTTTCCAA
          301 ATGTGCGCGC ACTGACCGTC AAGGGGAAAC CCCACTTCGC CGACTTTAAC CTGGTGCCCC
          361 CTGATTGGGG AGGATACGCC GGCCCTTGGA TCGAGGCAGC CGCTCGCGGC TGTCATGGAC
          421 TGGAGGAACT GCNCATGAAG CGAATGGTGG TCTCTGACNA AAGNCTGGAN CTGCTGGCTC
          481 GGANNTNNCC TAGGNNNCNN GCACTGGTGC NNNNNNCTTG CNNAGGCTTC NNCACCNANG
        541 GNCTGGCNNC NNNNNNNNNN
        //
        Primer: OsTIR1-B_R
        Region: OsTIR1 (WT) 3', P2A,mAID,EGFP 5'
        Sequence file: RDB18333_B0Gfb.seq check
        >18333_18333_B0Gf_3_OsTIR1-B_R_B03_06_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNAT GGANGAAANC NTGGCGACCT GCCNAAGGTG GAGAAACTGT
           61 ATGTGTATCG GACCACCGCA GGGGCAAGAG ATGATGCTCC CAACTTTGTG AAGATTCTGA
          121 GATCAGGAAG CGGAGCTACT AACTTCAGCC TGCTGAAGCA GGCTGGAGAC GTGGAGGAGA
          181 ACCCTGGACC TAGATCTAAG GAGAAGAGTG CTTGTCCTAA AGATCCAGCC AAACCTCCGG
          241 CCAAGGCACA AGTTGTGGGA TGGCCACCGG TGAGATCATA CCGGAAGAAC GTGATGGTTT
          301 CCTGCCAAAA ATCAAGCGGT GGCCCGGAGG CGGCGGCGTT CGTGAAGGTA TCAATGGACG
          361 GAGCACCGTA CTTGAGGAAA ATCGATTTGA GGATGTATAA AGCTAGCATG GTGAGCAAGG
          421 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG
          481 GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC
          541 TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACNC
          601 TGACCTACNG CGTGCAGTGC TTCNGCCGCT ACCCCGACCA CNTGAANCAG CACGACTTCT
          661 TCNAGTCCGC CATGCCCGNN NNTACGTNNN GNANCGCACC ATCNTCNTCA NGNNNNNNGC
        721 NNNTNCANNN NCCGCNNNNA NNNNNANNTN NNNNNNNNN
        //
        Primer: EGFP-C2
        Region: EGFP 3', Linker, NLS, SV40 pA, loxP,mPGK pro
        Sequence file: RDB18333_B0Gfc.seq check
        >18333_18333_B0Gf_3_EGFP-C2_C01_07_ABI24.ab1
            1 NNNNNNNNNN NCGCNNNCNC NTGGTCCTGC TGGANTTCGT GACCGCCGCC GGGATCACTC
           61 TCGGCATGGA CGAGCTGTAC AAGGGTGCAG GCGCCCAAGC TTCGAATTCT GCAGTCGACC
          121 CCAAGAAGAA AAGAAAGGTC ATCGACGGTA CCGCGGGCCC GGGATCCACC GGATCTAGAT
          181 AACTGATCAT AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC
          241 CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT AACTTGTTTA
          301 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT
          361 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TAAGCGATCA
          421 TATTCAATAA CCCTTAATAT AACTTCGTAT AATGTATGCT ATACGAAGTT ATTAGGTCTG
          481 AAGAGGAGTT TACGTCCAGC CAAGCTTAGG ATCTCGACCT CGAAATTCTA CCGGGTAGGG
          541 GAGGCGCTTT TCCCAAGGCA GTCTGGAGCA TGCGCTTTAG CAGCCCCGCT GGGCACTTGN
          601 CGCTACACAA GTGGCCTCTG GCCTCGCACA CATTCCACAT CCACCGGTAN GCGCCAACCG
          661 ACTCCGTTCN TTGGNGGNCC CNTCNNNCCN CCTTCNACTC CNCCCNNAGT NNNNNAGNNN
        721 CCNCCNNNNN NNNNN
        //
        Primer: pUC-M13_-100
        Region: Tol2R175, loxP, mPGK ter
        Sequence file: RDB18333_B0Gfd.seq check
        >18333_18333_B0Gf_3_pUC-M13_-100_E01_13_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN GGGNNNNGCN NGNGTTTTCC CNGTCACGAC GTTGTAAAAC
           61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGGTA CCGGCATATG
          121 GTTCTTGACA GAGGTGTAAA AAGTACTCAA AAATTTTACT CAAGTGAAAG TACAAGTACT
          181 TAGGGAAAAT TTTACTCAAT TAAAAGTAAA AGTATCTGGC TAGAATCTTA CTTGAGTAAA
          241 AGTAAAAAAG TACTCCATTA AAATTGTACT TGAGTATTAA GGAAGTAAAA GTAAAAGCAA
          301 GAAAGATCTT AGTGAACCTC TTCGAGGGAC CTAATAACTT CGTATAGCAT ACATTATACG
          361 AAGTTATATT AAGGGTTCCG GATCTCGACC AGCTTCTGAT GGAATTAGAA CTTGGCAAAA
          421 CAATACTGAG AATGAAGTGT ATGTGGAACA GAGGCTGCTG ATCTCGTTCT TCAGGCTATG
          481 AAACTGACAC ATTTGGAAAC CACAGTACTT AGAACCACAA AGTGGGAATC AAGAGAAAAA
          541 CAATGATCCC ACGAGAGATC TATAGATCTA TAGATCATGA GTGGGAGGAA TGAGCTGGCC
          601 CTTAATTTGG TTTTGNTTGT TTAAATTATG ATATCCAACT ATGAAACATT ATCNTNNNCN
          661 ATAGTAAAGA NCCTTCNGTA AAGAGCANGC NNTTATCNNN TCCCACCCCN NNCCCCNCCC
        721 CCNNNANNNC CNANNCNNNN NNTNNNAANN NNN
        //
        Primer: PGKpro_F
        Region: PuroR 5'
        Sequence file: RDB18333_B0Gfe.seq check
        >18333_18333_B0Gf_3_PKGpro_F_F01_16_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNTCTCCG GGCCTTTCGA CCTGCATCCN TCTAGATCTC
           61 GAGCAGCTGA AGCTTACCAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC CACCCGCGAC
          121 GACGTCCCCA GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC CGCCACGCGC
          181 CACACCGTCG ATCCGGACCG CCACATCGAG CGGGTCACCG AGCTGCAAGA ACTCTTCCTC
          241 ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC CGCGGTGGCG
          301 GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC
          361 ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG CCTCCTGGCG
          421 CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGCGTCTC GCCCGACCAC
          481 CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC CGANCGCGCC
          541 GGGNNGNCCG CCTTCCTGGA NACCTCCGCG CCCCACANCC NNCCCNTTCT ACGAGCGGCT
          601 CGGNTTNCNC NNNNNNCNNC GANGTCNAGG NGNNCCNANN GNNCNNNNNN CTGGTGCATG
          661 ANNCNNNNAG NCNNNTGCCT GNNNNCCGCC NCANNNNCCG NNNCGNCCNG NNNNNANNNN
        721 NNNNNN
        //
        Primer: reverse-2
        Region: T3 pro,Tol2L200, CMV en.
        Sequence file: RDB18333_B0Gff.seq check
        >18333_18333_B0Gf_3_reverse-2_D01_10_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNCGC GCNNTTAACC CTNACTAAAG GGAACAAAAG CTGGAGCTCG
           61 GATCTGCTGG GCTTGCTGAA GGTAGGGGGT CAAGAACCAG AGGTGTAAAG TACTTGAGTA
          121 ATTTTACTTG ATTACTGTAC TTAAGTATTA TTTTTGGGGA TTTTTACTTT ACTTGAGTAC
          181 AATTAAAAAT CAATACTTTT ACTTTTACTT AATTACATTT TTTTAGAAAA AAAAGTACTT
          241 TTTACTCCTT ACAATTTTAT TTACAGTCAA AAAGTACTTA TTTTTTGGAG ATCACTTGGG
          301 CCCTCTAGAT GCATGCTCGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG
          361 GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC
          421 CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA
          481 TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG
          541 CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCNATG
          601 ACGGTAAATG GCCCGCCTGG CNTTATGCCC AGTACATGAN CTTATGGGAN TTTCCTACTT
          661 GGCAGTACAT CTACNTANTA NTCNTCGCTA NNANNNNGGN CNANGTGAGC CCCNCNNTNN
        721 NGNNTNNNNN CNCCCNNNNN NN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522.

        2022.08.12

        GNP_filter3_RDBDEP_html_220812.pl