Sequencing and PCR primers for primer information.
A CRISPR/Cas plasmid for making DNA dounle-strand break at the human AAVS1 locus.
| Catalog number | RDB13917 |
|---|---|
| Resource name | AAVS1 T2 CRISPR in pX330 |
| Clone info. | A CRISPR/Cas plasmid for making DNA dounle-strand break at the human AAVS1 locus. |
| Vector backbone | pX330-U6-Chimeric_BB-CBh-hSpCas9 (Plasmid) |
| Size of vector backbone | 4.3 kb |
| Selectable markers | Am^r |
| Gene/insert name | S. pyogenes. Cas9 cDNA |
| Depositor | Kanemaki, Masato | |
| Other clones in our bank | S. pyogenes. (KEGG orthology K09952) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Exclusive MTA (genome editing) [Word] |
| Remarks | Please use the Exclusive MTA (genome editing). [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB13917 | AAVS1 T2 CRISPR in pX330 | DNA solution |
Electronic file
Featured content
| Featured content | Auxin Inducible Degron (AID) System (English text) |
|---|---|
| Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
References
| original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB13917_A6B8p1_v2.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: SV40pA3'Element Reverse | RDB13917_A6B8a_2.seq |
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>D03070A01_A6B8_1_SV40pA3'element_Reverse_G12_21.ab1 1 GGCGCAGGAG GCGGACCTAT GGAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 61 TTTGCTGGCC TTTTGCTCAC ATGTGAGGGC CTATTTCCCA TGATTCCTTC ATATTTGCAT 121 ATACGATACA AGGCTGTTAG AGAGATAATT GGAATTAATT TGACTGTAAA CACAAAGATA 181 TTAGTACAAA ATACGTGACG TAGAAAGTAA TAATTTCTTG GGTAGTTTGC AGTTTTAAAA 241 TTATGTTTTA AAATGGACTA TCATATGCTT ACCGTAACTT GAAAGTATTT CGATTTCTTG 301 GCTTTATATA TCTTGTGGAA AGGACGAAAC ACCGGGGCCA CTAGGGACAG GATGTTTTAG 361 AGCTAGAAAT AGCAAGTTAA AATAAGGCTA GTCCGTTATC AACTTGAAAA AGTGGCACCG 421 AGTCGGTGCT TTTTTGTTTT AGAGCTAGAA ATAGCAAGTT AAAAATAAAG GCTAGTCCGT 481 TTTAGCGCGT GCGCCAATTC TGCAGAACAA ATGGCTCTAG AAGGTACCCG TTACCATAAC 541 TTTACGGTAA AATGGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCCGCCC CATTGACGTC 601 AATAGTAACG CCCAATAGGG ACCTTTCCAT TGAACGTCAA GGGGTGAGGA ATTACGCGTA 661 AAACTGCCAC TGGGCGGACA TACAGTGTGA TCTATTGCCA GGTACGCCCC CTATGACGTC 721 ATGACGTTAA TGGCCCCTGC TTTGTCCATA CATGACCTAT GGAACTTCTC ACTGGCGAAC 781 TCAACGATTA GACCGCCGAT ACATGGCGAG TTGATCAAAG TTTTTGTTTT TTATTTCTCA 841 GGATGCGCGC GCGTTGCGAA CCCCCAATTT GGAAATATA // |
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| Primer: RDB13535#1 | RDB13917_A6B8b_2.seq |
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>D03070A01_A6B8_1_RDB13535#1_H12_24.ab1 1 TTGACATGCC TGAATCACTT TTTTTCAGGT TGGACCGGTG CCACCATGGA CTATAAGGAC 61 CACGACGGAG ACTACAAGGA TCATGATATT GATTACAAAG ACGATGACGA TAAGATGGCC 121 CCAAAGAAGA AGCGGAAGGT CGGTATCCAC GGAGTCCCAG CAGCCGACAA GAAGTACAGC 181 ATCGGCCTGG ACATCGGCAC CAACTCTGTG GGCTGGGCCG TGATCACCGA CGAGTACAAG 241 GTGCCCAGCA AGAAATTCAA GGTGCTGGGC AACACCGACC GGCACAGCAT CAAGAAGAAC 301 CTGATCGGAG CCCTGCTGTT CGACAGCGGC GAAACAGCCG AGGCCACCCG GCTGAAGAGA 361 ACCGCCAGAA GAAGATACAC CAGACGGAAG AACCGGATCT GCTATCTGCA AGAGATCTTC 421 AGCAACGAGA TGGCCAAGGT GGACGACAGC TTCTTCCACA GACTGGAAGA GTCCTTCCTG 481 GTGGAAGAGG ATAAGAAGCA CGAGCGGCAC CCCATCTTCG GCAACATCGT GGACGAGGTG 541 GCCTACCACG AGAAGTACCC CACCATCTAC CACCTGAGAA AGAAACTGGT GGACAGCACC 601 GACAAGGCCG ACCTGCGGCT GATCTATCTG GCCCTGGCCC ACATGATCAA GTTCCGGGGC 661 CACTTCCTGA TCGAGGGCGA CCTGAACCCC GACAACAGCG ACGTGGACAA GCTGTTCATC 721 CAGCTGGTGC AGACCTACAA CCAGCTGTTC GAGGAAAACC CCATCAACGC CAGCGGCGTG 781 GACGCCAAGG CCATCCTGTC TGCCAGACTG AGCAAGAGCA GACGGCTGGA AAATCTGATC 841 GCCCAGCTGC CCGGCGAGAA GAAGAATGGC CTGTTCGGAA ACCTGATTGC CCTGAGCCTG 901 GGCCTGACCC CCAACTTCAA GAGCAACTTC GACCTGGCCC GAGGATGCCA AACTGCAGCT 961 GAGCAAGGAC ACCTACGACG ACGACCTGGA CAAACCTGCT GGCCAAGATC GGCGACCAGT 1021 ACGCCCGACC TTGTTTCTGG CCGCCAAGAA CCTTGTCCGG ACGCCATCAT GCCTGACCGG 1081 ACATCCTGGA GAGTTGACAC GAGATCAACT AAGCACCCCT GGACGGCCTC TTATGGAATT 1141 CCAAG // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. |
|---|---|
| reference | Natsume, T., Acute inactivation of the replicative helicase in human cells triggers MCM8-9-dependent DNA synthesis. Genes Dev. (2017). PMID 28487407. |
2018.12.10
GNP_filter3_RDBDEP_html_181012.pl