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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

AAVS1 T2 CRISPR in pX330

A CRISPR/Cas plasmid for making DNA dounle-strand break at the human AAVS1 locus.

Catalog number RDB13917
Resource name AAVS1 T2 CRISPR in pX330
Clone info. A CRISPR/Cas plasmid for making DNA dounle-strand break at the human AAVS1 locus.
Vector backbone pX330-U6-Chimeric_BB-CBh-hSpCas9 (Plasmid)
Size of vector backbone 4.3 kb
Selectable markers Am^r
Gene/insert name S. pyogenes. Cas9 cDNA
Depositor|Developer Kanemaki, Masato |
 
Other clones in our bank S. pyogenes. (KEGG orthology K09952) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Remarks
The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please refer the Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose).
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RDB13917 AAVS1 T2 CRISPR in pX330 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13917_A6B8p1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3'element Reverse
Region: U6 pro, gRNA, gRNA scaffold
Sequence file: RDB13917_A6B8c.seq check
>D03070A01_A6B8_1_SV40pA3'element_Reverse_G12_21.ab1
    1 GGCGCAGGAG GCGGACCTAT GGAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT
   61 TTTGCTGGCC TTTTGCTCAC ATGTGAGGGC CTATTTCCCA TGATTCCTTC ATATTTGCAT
  121 ATACGATACA AGGCTGTTAG AGAGATAATT GGAATTAATT TGACTGTAAA CACAAAGATA
  181 TTAGTACAAA ATACGTGACG TAGAAAGTAA TAATTTCTTG GGTAGTTTGC AGTTTTAAAA
  241 TTATGTTTTA AAATGGACTA TCATATGCTT ACCGTAACTT GAAAGTATTT CGATTTCTTG
  301 GCTTTATATA TCTTGTGGAA AGGACGAAAC ACCGGGGCCA CTAGGGACAG GATGTTTTAG
  361 AGCTAGAAAT AGCAAGTTAA AATAAGGCTA GTCCGTTATC AACTTGAAAA AGTGGCACCG
  421 AGTCGGTGCT TTTTTGTTTT AGAGCTAGAA ATAGCAAGTT AAAAATAAAG GCTAGTCCGT
  481 TTTAGCGCGT GCGCCAATTC TGCAGAACAA ATGGCTCTAG AAGGTACCCG TTACCATAAC
  541 TTTACGGTAA AATGGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCCGCCC CATTGACGTC
  601 AATAGTAACG CCCAATAGGG ACCTTTCCAT TGAACGTCAA GGGGTGAGGA ATTACGCGTA
  661 AAACTGCCAC TGGGCGGACA TACAGTGTGA TCTATTGCCA GGTACGCCCC CTATGACGTC
  721 ATGACGTTAA TGGCCCCTGC TTTGTCCATA CATGACCTAT GGAACTTCTC ACTGGCGAAC
  781 TCAACGATTA GACCGCCGAT ACATGGCGAG TTGATCAAAG TTTTTGTTTT TTATTTCTCA
841 GGATGCGCGC GCGTTGCGAA CCCCCAATTT GGAAATATA
//
Primer: RDB13535#1
Region: CBh pro, 3xFLAG, SV40 NLS, Cas9 5'
Sequence file: RDB13917_A6B8d.seq check
>D03070A01_A6B8_1_RDB13535#1_H12_24.ab1
    1 TTGACATGCC TGAATCACTT TTTTTCAGGT TGGACCGGTG CCACCATGGA CTATAAGGAC
   61 CACGACGGAG ACTACAAGGA TCATGATATT GATTACAAAG ACGATGACGA TAAGATGGCC
  121 CCAAAGAAGA AGCGGAAGGT CGGTATCCAC GGAGTCCCAG CAGCCGACAA GAAGTACAGC
  181 ATCGGCCTGG ACATCGGCAC CAACTCTGTG GGCTGGGCCG TGATCACCGA CGAGTACAAG
  241 GTGCCCAGCA AGAAATTCAA GGTGCTGGGC AACACCGACC GGCACAGCAT CAAGAAGAAC
  301 CTGATCGGAG CCCTGCTGTT CGACAGCGGC GAAACAGCCG AGGCCACCCG GCTGAAGAGA
  361 ACCGCCAGAA GAAGATACAC CAGACGGAAG AACCGGATCT GCTATCTGCA AGAGATCTTC
  421 AGCAACGAGA TGGCCAAGGT GGACGACAGC TTCTTCCACA GACTGGAAGA GTCCTTCCTG
  481 GTGGAAGAGG ATAAGAAGCA CGAGCGGCAC CCCATCTTCG GCAACATCGT GGACGAGGTG
  541 GCCTACCACG AGAAGTACCC CACCATCTAC CACCTGAGAA AGAAACTGGT GGACAGCACC
  601 GACAAGGCCG ACCTGCGGCT GATCTATCTG GCCCTGGCCC ACATGATCAA GTTCCGGGGC
  661 CACTTCCTGA TCGAGGGCGA CCTGAACCCC GACAACAGCG ACGTGGACAA GCTGTTCATC
  721 CAGCTGGTGC AGACCTACAA CCAGCTGTTC GAGGAAAACC CCATCAACGC CAGCGGCGTG
  781 GACGCCAAGG CCATCCTGTC TGCCAGACTG AGCAAGAGCA GACGGCTGGA AAATCTGATC
  841 GCCCAGCTGC CCGGCGAGAA GAAGAATGGC CTGTTCGGAA ACCTGATTGC CCTGAGCCTG
  901 GGCCTGACCC CCAACTTCAA GAGCAACTTC GACCTGGCCC GAGGATGCCA AACTGCAGCT
  961 GAGCAAGGAC ACCTACGACG ACGACCTGGA CAAACCTGCT GGCCAAGATC GGCGACCAGT
 1021 ACGCCCGACC TTGTTTCTGG CCGCCAAGAA CCTTGTCCGG ACGCCATCAT GCCTGACCGG
 1081 ACATCCTGGA GAGTTGACAC GAGATCAACT AAGCACCCCT GGACGGCCTC TTATGGAATT
1141 CCAAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.
reference Natsume, T., Acute inactivation of the replicative helicase in human cells triggers MCM8-9-dependent DNA synthesis. Genes Dev. (2017). PMID 28487407.

2021.03.11

GNP_filter3_RDBDEP_html_210308.pl