A CRISPR/Cas plasmid for making DNA dounle-strand break at the human AAVS1 locus.
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External Database S. pyogenes. Cas9 Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested. |
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Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word ![]() |
Remarks | The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations. |
提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。 付加的提供条件: CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。 |
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提供依頼 | 手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word ![]() 専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word] ![]() |
備考 | 本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB13917 | AAVS1 T2 CRISPR in pX330 | DNA solution |
Materials & Methods section:
The AAVS1 T2 CRISPR in pX330 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13917). |
Reference section:
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Auxin Inducible Degron (AID) System (English text) |
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Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13917_A6B8p1-4.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: SV40pA3'element Reverse (Pr0108) Region: U6 pro, gRNA, gRNA scaffold Sequence file: RDB13917_A6B8c.seq ![]() |
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>13917_13917_B2JjF_U6pro_F3_E02_14_ABI24.ab1 1 NNNNNNNNTN NNANNGGNNN ATNNTATGCT TACCGTAACT TGAAAGTATT TCGATTTCTT 61 GGCTTTATAT ATCTTGTGGA AAGGACGAAA CACCGGGGCC ACTAGGGACA GGATGTTTTA 121 GAGCTAGAAA TAGCAAGTTA AAATAAGGCT AGTCCGTTAT CAACTTGAAA AAGTGGCACC 181 GAGTCGGTGC TTTTTTGTTT TAGAGCTAGA AATAGCAAGT TAAAATAAGG CTAGTCCGTT 241 TTTAGCGCGT GCGCCAATTC TGCAGACAAA TGGCTCTAGA GGTACCCGTT ACATAACTTA 301 CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAGTAA 361 CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT 421 TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA 481 AATGGCCCGC CTGGCATTGT GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT 541 ACATCTACGT ATTAGTCATC GCTATTACCA TGGTCGAGGT GAGCCCCACG TTCTGCTTCA 601 CTCTCCCCAT CTCCCCCCCC TCCCCACCCC CAATTTTGTA TTTATTTATT TTTTAATTAT 661 TTTGTGCAGC GATGGGGGCG GGGGGGGGGG GGGGGNNCGC GCCAGGCGGG GCGGGGCGGG 721 NCNNGGGGCG GGGCGGGNCN AGGCGNANAG GTGCGGCGGC ANCCAATCAN AGCGGCGCGC 781 TCCGAAAGTT NCCTTTNATG GNNAGGNGNN GGCGGCGGCN NCCCTATAAA AAGCNNNNCG 841 CGCGGNNGGG CGGGANNCGC NGCGCNCTGC NTNNGCCCNG NNCNNNNTCC GNNNNNNNCN 901 TCGNNNNGCC CNNCCCNNGG CTCTNNNNGA NNNCGTNNNT NCNNNNNGNN ANNNGNNNGG 961 NNNNNNNNNN // |
Primer: RDB13535#1 (Pr0302) Region: CBh pro, 3xFLAG, SV40 NLS, Cas9 5' Sequence file: RDB13917_A6B8d.seq ![]() |
>13917_13917_B2JjF_Cas9-N_R1_E02_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNGN NNNNNNNNNT GTACTCGTCG GTGANCACGG CCCAGCCCAC 61 AGAGTTGGTG CCGATGTCCA GGCCGATGCT GTACTTCTTG TCGGCTGCTG GGACTCCGTG 121 GATACCGACC TTCCGCTTCT TCTTTGGGGC CATCTTATCG TCATCGTCTT TGTAATCAAT 181 ATCATGATCC TTGTAGTCTC CGTCGTGGTC CTTATAGTCC ATGGTGGCAC CGGTCCAACC 241 TGAAAAAAAG TGATTTCAGG CAGGTGCTCC AGGTAATTAA ACATTAATAC CCCACCAACC 301 AACCATCCCT TAAACCCTTA CCTCTTGCTC AGCTAATTAC AGCCCGGAGG AGAAGGGCCG 361 TCCCGCCCGC TCACCTGTGG GAGTAACGCG GTCAGTCAGA GCCGGGGCGG GCGGCGCGAG 421 GCGN // |
Primer: bGH_rev3 (Pr0885) Region: BGH pA,nucleoplasmin NLS,Cas9 Sequence file: RDB13917_A6B8e.seq ![]() |
>13917_13917_B2JjF_bGH_rev3_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGGG CNNNCAACAG ATGGCTGGCA ACTAGAAGGC 61 ACAGTCGAGG CTGATCAGCG AGCTCTAGGA ATTCTTACTT TTTCTTTTTT GCCTGGCCGG 121 CCTTTTTCGT GGCCGCCGGC CTTTTGTCGC CTCCCAGCTG AGACAGGTCG ATCCGTGTCT 181 CGTACAGGCC GGTGATGCTC TGGTGGATCA GGGTGGCGTC CAGCACCTCT TTGGTGCTGG 241 TGTACCTCTT CCGGTCGATG GTGGTGTCAA AGTACTTGAA GGCGGCAGGG GCTCCCAGAT 301 TGGTCAGGGT AAACAGGTGG ATGATATTCT CGGCCTGCTC TCTGATGGGC TTATCCCGGT 361 GCTTGTTGTA GGCGGACAGC ACTTTGTCCA GATTAGCGTC GGCCAGGATC ACTCTCTTGG 421 AGAACTCGCT GATCTGCTCG ATGATCTCGT CCAGGTAGTG CTTGTGCTGT TCCACAAACA 481 GCTGTTTCTG CTCATTATCC TCGGGGGAGC CCTTCAGCTT CTCATAGTGG CTGGCCAGGT 541 ACAGGAAGTT CACATATTTG GAGGGCAGGG CCAGTTCGTT TCCCTTCTGC AGTTCGCCGG 601 CAGAGGCCAG CATTCTCTTC CGGCCGTTTT CCAGCTCGAA CAGGGAGTAC TTAGGCAGCT 661 TGATGATCAG GTCCTTTTTC ACTTCTTTGT AGCCCTTGGC TTCCAGAAAG TCGATGGGAT 721 TCTTCTCGAA GCTGCTTCTT TCCATGATGG TGATCCCCAG CAGCTCTTTC ACACTCTTCA 781 GTTTCTTGGA CTTGCCCTTT TCCACTTTGG CCACCACCAG CACAGAATAN GCCACGGNGG 841 GGCTGTNNAA GCCGCCGTAC TTCTTANGGT CCCAGTCCTT CTTTCTGGCG ATCAGCTTAT 901 CNCTGTNNCN CTNNNNNANG ANANNCTCTT NGCTGNNNCC NNCTNNNTGC NNCNCNGNCN 961 TTTTCACNNN ANTNNNNNNN GGNNNNGNNN NNNNNNTNNN NNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. |
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reference | Natsume, T., Acute inactivation of the replicative helicase in human cells triggers MCM8-9-dependent DNA synthesis. Genes Dev. (2017). PMID 28487407. |
2023.05.22
GNP_filter3_RDBDEP_html_230512.pl