A plasmid for construction of C-terminal mAID tagging donors.
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External Database Arabidopsis thaliana AtIAA17 Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
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Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ![]() |
提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。 |
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提供依頼 | 手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word ![]() 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word ![]() |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB13929 | pMK286 (mAID-NeoR) | DNA solution |
Electronic file
Original reference
original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. |
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Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Auxin Inducible Degron (AID) System (English text) |
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Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13929_A6B8p1-3.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: M13_-40 Region: insert5'(T7 pro,Linker,mAID,SV40p(A),f1ori) Sequence file: RDB13929_A6B8d.seq ![]() |
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>D03070A13_A6B8_1_M13(-40)_F03_18.ab1 1 AGGCGTTACG CGCGTATACG ACTCACTATA GGGCGAATTG GAGCTCCCCG GGATCCGGTG 61 CAGGCGCCAA GGAGAAGAGT GCTTGTCCTA AAGATCCAGC CAAACCTCCG GCCAAGGCAC 121 AAGTTGTGGG ATGGCCACCG GTGAGATCAT ACCGGAAGAA CGTGATGGTT TCCTGCCAAA 181 AATCAAGCGG TGGCCCGGAG GCGGCGGCGT TCGTGAAGGT ATCAATGGAC GGAGCACCGT 241 ACTTGAGGAA AATCGATTTG AGGATGTATA AATAATCTAG ATAACTGATC ATAATCAGCC 301 ATACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT CCCACACCTC CCCCTGAACC 361 TGAAACATAA AATGAATGCA ATTGTTGTTG TTAACTTGTT TATTGCAGCT TATAATGGTT 421 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 481 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTAACGCGT AAATTGTAAG CGTTAATATT 541 TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA 601 ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA 661 GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC 721 GTCTATCAGG GCGATGGCCC CACTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC 781 GAGGTGCCTT AAAGCACTAA ATCGGAACCC TAAGGGAGCC CCCCATTTAA ACTTGACGGG 841 AAAATCCCGC GAACGTTGCC AGAAAGGAAA GGAAAAAAGA CAAAAGGACC GGGAGCTAGG 901 GCACTAGAAA GGCTAACGGG ACCCCCTGCA CTACCACCAC AAAAGCCGCG CTTACTTTCT 961 CCTGTCATGG ATTCACGGTG CGAAATTAGT CAGTAGAAGA AAACAGAACC CGATTATCAT 1021 CTCTTAACAG CAAAAACTCA AAAAATTAAG C // |
Primer: SV40pro_F_V2 Region: insert mid(Sv40pro_ori,Neo_KanR) Sequence file: RDB13929_A6B8e.seq ![]() |
>D03070A13_A6B8_1_SV40pro_F_V2_D10_10.ab1 1 GTACCACTTT TTTGGGAGGC TAGGCTTTTG CAAGATCGAT CAAGAGACAG GATGAGGATC 61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG 121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG 181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA 241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC 301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC 361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA 421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA 481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT 541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT 601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT 661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA 721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA 781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG 841 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GAAATGACCG ACCAAGCGAC 901 GCCCAAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AAGGTTGGGG 961 CTTCGGAATC GTTTTTCGGG ACGCCGGGCT GGATGATCCT CCAAGCGCGG GGGATCTCCA 1021 TGCTGGGAGG TTCTTTCGCC CCACCCCTAG GGGGAGGGCT TACTTGAGAC ACGGGAAGGA 1081 GACAATACCG GAAGGACCGC GCTATGAACG GCAATTAAAA GGACAGAACT TAAAACGCGC 1141 CCACCCG // |
Primer: M13R Region: insert3'(TKp(A),NeoR_KanR) Sequence file: RDB13929_A6B8f.seq ![]() |
>D03070A19_A6B8_1_M13R_D04_10.ab1 1 GCCGCGGCAT TACCTCACTA AAGGGAACAA AAGCTGGGTA CCGGATCCAA CGACCCAACA 61 CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC 121 TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC 181 CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA 241 TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC 301 ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC 361 GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC 421 CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGG TCCGCCACAC 481 CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA 541 AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC 601 TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA 661 CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA 721 ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA 781 CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA 841 GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG 901 TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA 961 GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCCGGAA CACGCGGCAT 1021 CAGAGCAGCC CGATTGTCTG TGTGCCCAGT CATAGGCCGG ATAGGCCTCT CCACCCCAAG 1081 GCGGGCCAGG AGATCTTGCG ATGCCAGTTC CAATTCTTGA TCAGTCATGC CGAAAACCGA 1141 TTCCCT // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. |
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reference | Chen, F.X., PAF1 regulation of promoter-proximal pause release via enhancer activation. Science 357 (6357): 1294-1298 (2017). PMID 28860207. |
reference | Natsume, T., Acute inactivation of the replicative helicase in human cells triggers MCM8-9-dependent DNA synthesis. Genes Dev. (2017). PMID 28487407. |
reference | Devrekanli, A., Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes. Methods in Molecular Biology 1369: 257-278 (2016). PMID 26519318. |
reference | Nishimura, K., Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Curr. Protoc. Cell Biol. \64: 20.9.1-16 (2014). PMID 25181302. |
2022.05.17
GNP_filter3_RDBDEP_html_220516.pl