Sequencing and PCR primers for primer information.
A plasmid for construction of C-terminal mAID tagging donors.
| Catalog number | RDB13929 |
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| Resource name | pMK286 (mAID-NeoR) |
| Alternative name | mAID-NeoR, 1006 |
| Clone info. | A plasmid for construction of C-terminal mAID tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone. Please note that this plasmid does not contain the loxP sites, which are intended for removal of the marker. Sequence analysis is not reliable around MluI site adjacent to SV40 poly(A) site probably because of tangled tertiary structure induced by unexpected palindrome sequences. To make 0.5 M stock solution, dissolve indole-3-acetic acid (IAA) (Sigma-Aldrich, 45533) or 1-naphthaleneacetic acid (NAA) (Sigma-Aldrich, 35745) in 100 % DMSO. IAA solution can be made in 100 % ethanol. Aliquots can be kept for 6 months in the dark at -20oC (Devrekanli, A., Kanemaki, M.T. Methods in Molecular Biology, 1369, 257-278, 2016). |
| Vector backbone | pBluescriptII KS- (Plasmid) |
| Size of vector backbone | 3.0 kb |
| Selectable markers | Am^r |
| Gene/insert name | Arabidopsis thaliana AtIAA17 cDNA |
| Depositor | Kanemaki, Masato | |
| Other clones in our bank | Arabidopsis thaliana AtIAA17 (KEGG orthology K14484) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB13929 | pMK286 (mAID-NeoR) | DNA solution |
Electronic file
Featured content
| Featured content | Auxin Inducible Degron (AID) System (English text) |
|---|---|
| Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
References
| original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB13929_A6B8p1_v2.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: M13_-40 | RDB13929_A6B8a_2.seq |
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>D03070A13_A6B8_1_M13(-40)_F03_18.ab1 1 AGGCGTTACG CGCGTATACG ACTCACTATA GGGCGAATTG GAGCTCCCCG GGATCCGGTG 61 CAGGCGCCAA GGAGAAGAGT GCTTGTCCTA AAGATCCAGC CAAACCTCCG GCCAAGGCAC 121 AAGTTGTGGG ATGGCCACCG GTGAGATCAT ACCGGAAGAA CGTGATGGTT TCCTGCCAAA 181 AATCAAGCGG TGGCCCGGAG GCGGCGGCGT TCGTGAAGGT ATCAATGGAC GGAGCACCGT 241 ACTTGAGGAA AATCGATTTG AGGATGTATA AATAATCTAG ATAACTGATC ATAATCAGCC 301 ATACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT CCCACACCTC CCCCTGAACC 361 TGAAACATAA AATGAATGCA ATTGTTGTTG TTAACTTGTT TATTGCAGCT TATAATGGTT 421 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 481 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTAACGCGT AAATTGTAAG CGTTAATATT 541 TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA 601 ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA 661 GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC 721 GTCTATCAGG GCGATGGCCC CACTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC 781 GAGGTGCCTT AAAGCACTAA ATCGGAACCC TAAGGGAGCC CCCCATTTAA ACTTGACGGG 841 AAAATCCCGC GAACGTTGCC AGAAAGGAAA GGAAAAAAGA CAAAAGGACC GGGAGCTAGG 901 GCACTAGAAA GGCTAACGGG ACCCCCTGCA CTACCACCAC AAAAGCCGCG CTTACTTTCT 961 CCTGTCATGG ATTCACGGTG CGAAATTAGT CAGTAGAAGA AAACAGAACC CGATTATCAT 1021 CTCTTAACAG CAAAAACTCA AAAAATTAAG C // |
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| Primer: SV40pro_F_V2 | RDB13929_A6B8b_2.seq |
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>D03070A13_A6B8_1_SV40pro_F_V2_D10_10.ab1 1 GTACCACTTT TTTGGGAGGC TAGGCTTTTG CAAGATCGAT CAAGAGACAG GATGAGGATC 61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG 121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG 181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA 241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC 301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC 361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA 421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA 481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT 541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT 601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT 661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA 721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA 781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG 841 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GAAATGACCG ACCAAGCGAC 901 GCCCAAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AAGGTTGGGG 961 CTTCGGAATC GTTTTTCGGG ACGCCGGGCT GGATGATCCT CCAAGCGCGG GGGATCTCCA 1021 TGCTGGGAGG TTCTTTCGCC CCACCCCTAG GGGGAGGGCT TACTTGAGAC ACGGGAAGGA 1081 GACAATACCG GAAGGACCGC GCTATGAACG GCAATTAAAA GGACAGAACT TAAAACGCGC 1141 CCACCCG // |
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| Primer: M13R | RDB13929_A6B8c_2.seq |
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>D03070A13_A6B8_1_M13R_B03_06.ab1 1 GTCGCGCAGT ACCTCACTAA AGGGAACAAA AGCTGGGTAC CGGATCCAAC GACCCAACAC 61 CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT 121 CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC 181 CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT 241 AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA 301 TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG 361 CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC 421 AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGGT CCGCCACACC 481 CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA 541 GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT 601 GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC 661 AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA 721 TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC 781 TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG 841 CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT 901 CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG 961 GGTCGGTCTT GACAAAAAGA ACCCGGGCGC CCCTGCGCTG ACAGCCCGGA ACACGGCGGC 1021 ATCAGAGCAG CCGATTGTCT GTGTGCCCAG TCATAGCCCG ATAGCCTCTC CACCCAGGCG 1081 GCTGAGAACT GCGTGCATCC ATCTTGGTCA TCATGCGAAC GATCCCTCAT CCTGTTCTTC 1141 TTTGGAAA // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. |
|---|---|
| reference | Chen, F.X., PAF1 regulation of promoter-proximal pause release via enhancer activation. Science 357 (6357): 1294-1298 (2017). PMID 28860207. |
| reference | Natsume, T., Acute inactivation of the replicative helicase in human cells triggers MCM8-9-dependent DNA synthesis. Genes Dev. (2017). PMID 28487407. |
| reference | Devrekanli, A., Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes. Methods in Molecular Biology 1369: 257-278 (2016). PMID 26519318. |
| reference | Nishimura, K., Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Curr. Protoc. Cell Biol. \64: 20.9.1-16 (2014). PMID 25181302 . |
2018.12.11
GNP_filter3_RDBDEP_html_181012.pl