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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK390 (Stag-3FLAG-BSD)

A plasmid for construction of C-terminal Stag-3FLAG-BSD tagging donors.

Catalog number RDB16818
Resource name pMK390 (Stag-3FLAG-BSD)
Clone info. A plasmid for construction of C-terminal Stag-3FLAG-BSD tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Depositor|Developer Kanemaki, Masato |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB16818 pMK390 (Stag-3FLAG-BSD) DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Auxin Inducible Degron (AID) System (English text)
            Featured content Auxin Inducible Degron (AID) System (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB16818_A8Lpp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pUC-M13_-100
            Sequence file: RDB16818_A8Lpa.seq check
            >D06157B5_A8LP_2_pUC-M13_-100_C05_08_ABI24.ab1
                1 GAACGGTCAG GCGGATTAGT TGGGTACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC
               61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGAGC TCGGATCCGG
              121 TGCAGGCGCC AAGGAAACCG CCGCTGCAAA ATTCGAACGC CAGCACATGG ACAGCCGATC
              181 CCGGGCTGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA
              241 TGACGATGAC AAGTAGTGTA CAAGTAATCT AGATAACTGA TCATAATCAG CCATACCACA
              301 TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT
              361 AAAATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA
              421 AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT
              481 TTGTCCAAAC TCATCAATGT ATCTTAACGC GTCGATCATA TTCAATAACC CTTAATATAA
              541 CTTCGTATAA TGTATGCTAT ACGAAGTTAT TAGGTCTGAA GAGGAGTTTA CGTCCAGCCA
              601 AGCTTAGGAT CTCGACCTCG AAATTCTACC GGGTAGGGGA GGCGCTTTTC CCAAGGCAGT
              661 CTGGAGCATG CGCTTTAGCA GCCCCGCTGG GCACTTGGCG CTACACAAGT GGCCTCTGGC
              721 CTCGCACACA TTCCACATCC ACCGGTAGGC GCCAACCGAC TCCGTTCTTT GGTGGCCCCT
              781 TCGCGCCACC TTCTACTCCT CCCCTAGTCA GGAAGTTCCC CCCCCGCCCC GCAGCTCGCG
              841 TCGTGCAGGA CGTGACAAAT GGAAGTAGCA CGTCTCACTA GTCTCGTGCA GATGGACAGC
              901 ACCGCTGAGC AATGGAAGCG GGTAGGCCTT TGGGGGCAGC GGCCCAATAG CAGCTTTGCT
              961 CCCTTCGCTT TCTGGGGCTC AGAGGCTGGG AAAGGGTGGT CCGGGGGCGG GCTCAGGCGG
             1021 CTCAGGGCGG GGCGGCGCCG ATGTTCTCGG AGCTAGCATC TGCACGCATC AAAGCGCACG
            1081 GATTCTTGAC TC
            //
            Primer: reverse
            Sequence file: RDB16818_A8Lpb.seq check
            >D06157B5_A8LP_2_reverse_D05_11_ABI24.ab1
                1 AAAACCCATT AACCCCTCAC TAAAGGGAAC AAAAGCTGGG TACCGGATCC TAGTGAACCT
               61 CTTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC GAAGTTATAT TAAGGGTTCC
              121 GGATCTCGAC CAGCTTCTGA TGGAATTAGA ACTTGGCAAA ACAATACTGA GAATGAAGTG
              181 TATGTGGAAC AGAGGCTGCT GATCTCGTTC TTCAGGCTAT GAAACTGACA CATTTGGAAA
              241 CCACAGTACT TAGAACCACA AAGTGGGAAT CAAGAGAAAA ACAATGATCC CACGAGAGAT
              301 CTATAGATCT ATAGATCATG AGTGGGAGGA ATGAGCTGGC CCTTAATTTG GTTTTGCTTG
              361 TTTAAATTAT GATATCCAAC TATGAAACAT TATCATAAAG CAATAGTAAA GAGCCTTCAG
              421 TAAAGAGCAG GCATTTATCT AATCCCACCC CACCCCCACC CCCGTAGCTC CAATCCTTCC
              481 ATTCAAAATG TAGGTACTCT GTTCTCACCC TTCTTAACAA AGTATGACAG GAAAAACTTC
              541 CATTTTAGTG GACATCTTTA TTGTTTAATA GATCATCAAT TTCTGCATCC CGGGGATCTG
              601 ATATCATCGA TTAGCCCTCC CACACATAAC CAGAGGGCAG CAATTCACGA ATCCCAACTG
              661 CCGTCGGCTG TCCATCACTG TCCTTCACTA TGGCTTTGAT CCCAGGATGC AGATCGAGAA
              721 GCACCTGTCG GCACCGTCCG CAGGGGCTCA AGATGCCCCT GTTCTCATTT CCGATCGCGA
              781 CGATACAAGT CAGGTTGCCA GCTGCCGCAG CAGCAGCAGT GCCCAGCACC ACGAGTTCTG
              841 CACAAGGTCC CCCCAGTAAA ATGATATACA TTGACACCAG TGAAGATGCG GCCGTCGCTA
              901 GAGAGAGCTG CGCTGGCGAC GCTGTAGTCT TCAGAGATGG GGGATGCTGT TGATTGTAGC
              961 CGTTGCTCTT TCAATGAAGG GTGGGATTTC TTTCTTTGAG ACAAAGGCCT TGGCCATGGA
             1021 TGGCTCGGAG ATCTAGATGG GAATGCCAGT TCGTAAGGGT CCGGAGATGA GGAAGAGGAA
            1081 GAACAGCGCC GTCAGAACGG ATGACGGC
            //
            Primer: PGKpro_F
            Sequence file: RDB16818_A8Lpc.seq check
            >D06157B5_A8LP_2_PGKpro_F_E05_14_ABI24.ab1
                1 AGTGGGGGCC GATCTCTCTT CCTCATCTCC GGGCCTTTCG ACCTGCATCC ATCTAGATCT
               61 CGAGCCACCA TGGCCAAGCC TTTGTCTCAA GAAGAATCCA CCCTCATTGA AAGAGCAACG
              121 GCTACAATCA ACAGCATCCC CATCTCTGAA GACTACAGCG TCGCCAGCGC AGCTCTCTCT
              181 AGCGACGGCC GCATCTTCAC TGGTGTCAAT GTATATCATT TTACTGGGGG ACCTTGTGCA
              241 GAACTCGTGG TGCTGGGCAC TGCTGCTGCT GCGGCAGCTG GCAACCTGAC TTGTATCGTC
              301 GCGATCGGAA ATGAGAACAG GGGCATCTTG AGCCCCTGCG GACGGTGCCG ACAGGTGCTT
              361 CTCGATCTGC ATCCTGGGAT CAAAGCCATA GTGAAGGACA GTGATGGACA GCCGACGGCA
              421 GTTGGGATTC GTGAATTGCT GCCCTCTGGT TATGTGTGGG AGGGCTAATC GATGATATCA
              481 GATCCCCGGG ATGCAGAAAT TGATGATCTA TTAAACAATA AAGATGTCCA CTAAAATGGA
              541 AGTTTTTCCT GTCATACTTT GTTAAGAAGG GTGAGAACAG AGTACCTACA TTTTGAATGG
              601 AAGGATTGGA GCTACGGGGG TGGGGGTGGG GTGGGATTAG ATAAATGCCT GCTCTTTACT
              661 GAAGGCTCTT TACTATTGCT TTATGATAAT GTTTCATAGT TGGATATCAT AATTTAAACA
              721 AGCAAAACCA AATTAAGGGC CAGCTCATTC CTCCCACTCA TGATCTATAG ATCTATAGAT
              781 CTCTCGTGGG ATCATTGTTT TTCTCTTGAT TCCCACTTTG TGGTTCTAAG TACTGTGGTT
              841 TCCAAATGTG TCAGTTTCAT AGCCTGAAGA ACGAGATCAG CAGCCTCTGT TCCACATACA
              901 CTTCATTCTC AGTATTGTTT TTGCCAAGTT TCTAATTCCA TCAGAAGCTG GGTCGAGATC
              961 CGGAACCCTT TAAATATAAC TTCGTATAAT GTATGCTATA CGAAGTTTAT TAGGTCCCTC
             1021 GAGGAGGGTT TCACTAGGAT CCCGGTACCC AGCCTTTTTG ATTTCCCTTT TAAGTTGGAG
            1081 GATAATTTGC ACGCTGGACG TTAATCATGT CACTAGCTTG GATTATCG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

            2021.11.02

            GNP_filter3_RDBDEP_html_211029.pl