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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK390 (Stag-3FLAG-BSD)

A plasmid for construction of C-terminal Stag-3FLAG-BSD tagging donors.

Catalog number RDB16818
Resource name pMK390 (Stag-3FLAG-BSD)
Clone info. A plasmid for construction of C-terminal Stag-3FLAG-BSD tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Depositor|Developer Kanemaki, Masato |
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External Database

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB16818 pMK390 (Stag-3FLAG-BSD) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pMK390 (Stag-3FLAG-BSD) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16818).

            Reference section:

            Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

            Further references such as user reports and related articles (go to bottom)


            References and tips

            Featured content

            Featured content Auxin Inducible Degron (AID) System (English text)
            Featured content Auxin Inducible Degron (AID) System (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB16818_A8Lpp1-3.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pUC-M13_-100 (Pr0310)
            Region: T7 pro,insert 5'(Linker,S-tag,3XFLAG,SV40 pA,loxP,mPGK pro)
            Sequence file: RDB16818_A8Lpd.seq check
            >D06157B5_A8LP_2_pUC-M13_-100_C05_08_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN TNNGNNNGCC NGGNTTTTCC CNGTCNCGAC GTTGTAAAAC
               61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGAGC TCGGATCCGG
              121 TGCAGGCGCC AAGGAAACCG CCGCTGCAAA ATTCGAACGC CAGCACATGG ACAGCCGATC
              181 CCGGGCTGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA
              241 TGACGATGAC AAGTAGTGTA CAAGTAATCT AGATAACTGA TCATAATCAG CCATACCACA
              301 TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT
              361 AAAATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA
              421 AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT
              481 TTGTCCAAAC TCATCAATGT ATCTTAACGC GTCGATCATA TTCAATAACC CTTAATATAA
              541 CTTCGTATAA TGTATGCTAT ACGAAGTTAT TAGGTCTGAA GAGGAGTTTA CGTCCAGCCA
              601 AGCTTAGGAT CTCGACCTCG AAATTCTACC GGGTAGGGGA GGCGCTTTTC CCAAGGCAGT
              661 CTGGAGCATG CGCTTTANCA GCCCCGCTGG GCACTTGGCG CTACACAAGT GGCCTCTGGC
              721 CTCGCACACA TTCNACATCC ACCGGTANGC GCCAACCGAC TCCGTTCTTT GGTGGNCCCT
              781 TCGCGCCACC TTCTACTCCT CCCCTAGTCA NGAANTTCCC CCCCCGCCCC GCANCTCGCG
              841 NCGNGCANGN NGTGACAAAN GGAANTANCA CGNCNCACTA NNCTCGTGCA NATGGNNAGC
            901 ACNNCNNNNC NNNGGAANCG GGNNNNNNNT TNNGNNNANC NNNNNNNNNN
            //
            Primer: SV40pA3element Reverse (Pr0108)
            Region: lac pro,T3 pro,insert 3'(loxP,mouse PGK ter)
            Sequence file: RDB16818_A8Lpe.seq check
            >16818_16818_B3C8F_SV40pA3'element Reverse_F01_06_ABI08.ab1
                1 NNNNNNNNNN NNGGNNNNNN NNNNATGNNN NNNNCCNGCA ACGCGGCCTT TTTACGGTTC
               61 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG
              121 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG
              181 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC
              241 GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC
              301 AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC
              361 TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA
              421 AACAGCTATG ACCATGATTA CGCCAAGCGC GCAATTAACC CTCACTAAAG GGAACAAAAG
              481 CTGGGTACCG GATCCTAGTG AACCTCTTCG AGGGACCTAA TAACTTCGTA TAGCATACAT
              541 TATACGAAGT TATATTAAGG GTTCCGGATC TCGACCAGCT TCTGATGGAA TTAGAACTTG
              601 GCAAAACAAT ACTGAGAATG AAGTGTATGT GGAACAGAGG CTGCTGATCT CGTTCTTCAG
              661 GCTATGAAAC TGACACATTT GGAAACCACA GTACTTAGAA CCACAAAGTG GGAATCAAGA
              721 GAAAAACAAT GATCCCACGA GAGATCTATA GATCTATAGA TCATGANTGG GAGGAATGAG
              781 CTGGNCCTTA ATTTGGTTTT GCTTGTTTNA ATTATGATAT CCAACTATGA NNCATTATCA
              841 TAAAGCAATA GTAAAGAGCC TTCAGNNAAA GAGCANGNAT TTATCTAATC CCNCCCNNCC
              901 CCCNCCCCCG TAGCTNCNAT CCTTNNNTNC AAAANGTNNN NNCTCNGNNN NNNCNCNTCT
              961 TNNCNNNTAT GANNNNAAAA ACNTNNNNTT TTNNNNNNNT NNTTATNNNT TNNNNNNNNN
            1021 N
            //
            Primer: PGKpro_F (Pr0249)
            Region: insert mid(BSD,mouse PGK ter)
            Sequence file: RDB16818_A8Lpf.seq check
            >D06157B5_A8LP_2_PGKpro_F_E05_14_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTCC NGGCCTTTCG ACCTGCATCC NTCTAGATCT
               61 CGAGCCACCA TGGCCAAGCC TTTGTCTCAA GAAGAATCCA CCCTCATTGA AAGAGCAACG
              121 GCTACAATCA ACAGCATCCC CATCTCTGAA GACTACAGCG TCGCCAGCGC AGCTCTCTCT
              181 AGCGACGGCC GCATCTTCAC TGGTGTCAAT GTATATCATT TTACTGGGGG ACCTTGTGCA
              241 GAACTCGTGG TGCTGGGCAC TGCTGCTGCT GCGGCAGCTG GCAACCTGAC TTGTATCGTC
              301 GCGATCGGAA ATGAGAACAG GGGCATCTTG AGCCCCTGCG GACGGTGCCG ACAGGTGCTT
              361 CTCGATCTGC ATCCTGGGAT CAAAGCCATA GTGAAGGACA GTGATGGACA GCCGACGGCA
              421 GTTGGGATTC GTGAATTGCT GCCCTCTGGT TATGTGTGGG AGGGCTAATC GATGATATCA
              481 GATCCCCGGG ATGCAGAAAT TGATGATCTA TTAAACAATA AAGATGTCCA CTAAAATGGA
              541 AGTTTTTCCT GTCATACTTT GTTAAGAAGG GTGAGAACAG AGTACCTACA TTTTGAATGG
              601 AAGGATTGGA GCTACGGGGG TGGGGGTGGG GTGGGATTAG ATAAATGCCT GCTCTTTACT
              661 GAAGGCTCTT TACTATTGCT TTATGATAAT GTTTCATAGT TGGATATCAT AATTTAAACA
              721 AGCAAAACCA AATTAAGGGC CAGCTCATTC CTCCCACTCA TGATCTATAG ATCTATAGAT
              781 CTCTCGTGGG ATCATTGTTT TTCTCTTGAT TCCNACTTTG TGGTTCTAAG TACTGTGGTT
              841 NNNAAATGTG TCAGTTTCAT AGCCTGAAGA ANGAGATCAG CAGCNTCTGT TCCACATACN
              901 CTNCATTCTC ANNATTGNTT TNGNCNNGNT NCTANNTNNN TCNNNNNNTN GNNNNNNNNN
            961 N
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

            2023.05.01

            GNP_filter3_RDBDEP_html_230501.pl