A plasmid for construction of C-terminal Stag-3FLAG-BSD tagging donors.
Clone info. | A plasmid for construction of C-terminal Stag-3FLAG-BSD tagging donors. |
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Vector backbone | pBluescript (plasmid) |
Size of vector backbone | 2.9 kb |
Selectable markers | Am^r (E. coli) |
Growth conditions | LB+Amp, 37oC |
Depositor|Developer | Kanemaki, Masato | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() MTA, for use for for-profit purpose [Word ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 提供同意書 (MTA, 営利目的用)[Word ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB16818 | pMK390 (Stag-3FLAG-BSD) | DNA solution |
Materials & Methods section:
The pMK390 (Stag-3FLAG-BSD) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16818). |
Reference section:
Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB16818_A8Lpp1-3.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pUC-M13_-100 (Pr0310) Region: T7 pro,insert 5'(Linker,S-tag,3XFLAG,SV40 pA,loxP,mPGK pro) Sequence file: RDB16818_A8Lpd.seq ![]() |
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>D06157B5_A8LP_2_pUC-M13_-100_C05_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN TNNGNNNGCC NGGNTTTTCC CNGTCNCGAC GTTGTAAAAC 61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGAGC TCGGATCCGG 121 TGCAGGCGCC AAGGAAACCG CCGCTGCAAA ATTCGAACGC CAGCACATGG ACAGCCGATC 181 CCGGGCTGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA 241 TGACGATGAC AAGTAGTGTA CAAGTAATCT AGATAACTGA TCATAATCAG CCATACCACA 301 TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT 361 AAAATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA 421 AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT 481 TTGTCCAAAC TCATCAATGT ATCTTAACGC GTCGATCATA TTCAATAACC CTTAATATAA 541 CTTCGTATAA TGTATGCTAT ACGAAGTTAT TAGGTCTGAA GAGGAGTTTA CGTCCAGCCA 601 AGCTTAGGAT CTCGACCTCG AAATTCTACC GGGTAGGGGA GGCGCTTTTC CCAAGGCAGT 661 CTGGAGCATG CGCTTTANCA GCCCCGCTGG GCACTTGGCG CTACACAAGT GGCCTCTGGC 721 CTCGCACACA TTCNACATCC ACCGGTANGC GCCAACCGAC TCCGTTCTTT GGTGGNCCCT 781 TCGCGCCACC TTCTACTCCT CCCCTAGTCA NGAANTTCCC CCCCCGCCCC GCANCTCGCG 841 NCGNGCANGN NGTGACAAAN GGAANTANCA CGNCNCACTA NNCTCGTGCA NATGGNNAGC 901 ACNNCNNNNC NNNGGAANCG GGNNNNNNNT TNNGNNNANC NNNNNNNNNN // |
Primer: SV40pA3element Reverse (Pr0108) Region: lac pro,T3 pro,insert 3'(loxP,mouse PGK ter) Sequence file: RDB16818_A8Lpe.seq ![]() |
>16818_16818_B3C8F_SV40pA3'element Reverse_F01_06_ABI08.ab1 1 NNNNNNNNNN NNGGNNNNNN NNNNATGNNN NNNNCCNGCA ACGCGGCCTT TTTACGGTTC 61 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG 121 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG 181 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC 241 GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC 301 AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC 361 TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA 421 AACAGCTATG ACCATGATTA CGCCAAGCGC GCAATTAACC CTCACTAAAG GGAACAAAAG 481 CTGGGTACCG GATCCTAGTG AACCTCTTCG AGGGACCTAA TAACTTCGTA TAGCATACAT 541 TATACGAAGT TATATTAAGG GTTCCGGATC TCGACCAGCT TCTGATGGAA TTAGAACTTG 601 GCAAAACAAT ACTGAGAATG AAGTGTATGT GGAACAGAGG CTGCTGATCT CGTTCTTCAG 661 GCTATGAAAC TGACACATTT GGAAACCACA GTACTTAGAA CCACAAAGTG GGAATCAAGA 721 GAAAAACAAT GATCCCACGA GAGATCTATA GATCTATAGA TCATGANTGG GAGGAATGAG 781 CTGGNCCTTA ATTTGGTTTT GCTTGTTTNA ATTATGATAT CCAACTATGA NNCATTATCA 841 TAAAGCAATA GTAAAGAGCC TTCAGNNAAA GAGCANGNAT TTATCTAATC CCNCCCNNCC 901 CCCNCCCCCG TAGCTNCNAT CCTTNNNTNC AAAANGTNNN NNCTCNGNNN NNNCNCNTCT 961 TNNCNNNTAT GANNNNAAAA ACNTNNNNTT TTNNNNNNNT NNTTATNNNT TNNNNNNNNN 1021 N // |
Primer: PGKpro_F (Pr0249) Region: insert mid(BSD,mouse PGK ter) Sequence file: RDB16818_A8Lpf.seq ![]() |
>D06157B5_A8LP_2_PGKpro_F_E05_14_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTCC NGGCCTTTCG ACCTGCATCC NTCTAGATCT 61 CGAGCCACCA TGGCCAAGCC TTTGTCTCAA GAAGAATCCA CCCTCATTGA AAGAGCAACG 121 GCTACAATCA ACAGCATCCC CATCTCTGAA GACTACAGCG TCGCCAGCGC AGCTCTCTCT 181 AGCGACGGCC GCATCTTCAC TGGTGTCAAT GTATATCATT TTACTGGGGG ACCTTGTGCA 241 GAACTCGTGG TGCTGGGCAC TGCTGCTGCT GCGGCAGCTG GCAACCTGAC TTGTATCGTC 301 GCGATCGGAA ATGAGAACAG GGGCATCTTG AGCCCCTGCG GACGGTGCCG ACAGGTGCTT 361 CTCGATCTGC ATCCTGGGAT CAAAGCCATA GTGAAGGACA GTGATGGACA GCCGACGGCA 421 GTTGGGATTC GTGAATTGCT GCCCTCTGGT TATGTGTGGG AGGGCTAATC GATGATATCA 481 GATCCCCGGG ATGCAGAAAT TGATGATCTA TTAAACAATA AAGATGTCCA CTAAAATGGA 541 AGTTTTTCCT GTCATACTTT GTTAAGAAGG GTGAGAACAG AGTACCTACA TTTTGAATGG 601 AAGGATTGGA GCTACGGGGG TGGGGGTGGG GTGGGATTAG ATAAATGCCT GCTCTTTACT 661 GAAGGCTCTT TACTATTGCT TTATGATAAT GTTTCATAGT TGGATATCAT AATTTAAACA 721 AGCAAAACCA AATTAAGGGC CAGCTCATTC CTCCCACTCA TGATCTATAG ATCTATAGAT 781 CTCTCGTGGG ATCATTGTTT TTCTCTTGAT TCCNACTTTG TGGTTCTAAG TACTGTGGTT 841 NNNAAATGTG TCAGTTTCAT AGCCTGAAGA ANGAGATCAG CAGCNTCTGT TCCACATACN 901 CTNCATTCTC ANNATTGNTT TNGNCNNGNT NCTANNTNNN TCNNNNNNTN GNNNNNNNNN 961 N // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP] |
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