Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK390 (Stag-3FLAG-BSD) (#RDB16818)

A plasmid for construction of C-terminal Stag-3FLAG-BSD tagging donors.

Clone info. A plasmid for construction of C-terminal Stag-3FLAG-BSD tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Growth conditions LB+Amp, 37oC
Depositor|Developer Kanemaki, Masato |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16818 pMK390 (Stag-3FLAG-BSD) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMK390 (Stag-3FLAG-BSD) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16818).

Reference section:

Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16818_A8Lpp1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310)
Region: T7 pro,insert 5'(Linker,S-tag,3XFLAG,SV40 pA,loxP,mPGK pro)
Sequence file: RDB16818_A8Lpd.seq check
>D06157B5_A8LP_2_pUC-M13_-100_C05_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN TNNGNNNGCC NGGNTTTTCC CNGTCNCGAC GTTGTAAAAC
   61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGAGC TCGGATCCGG
  121 TGCAGGCGCC AAGGAAACCG CCGCTGCAAA ATTCGAACGC CAGCACATGG ACAGCCGATC
  181 CCGGGCTGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA
  241 TGACGATGAC AAGTAGTGTA CAAGTAATCT AGATAACTGA TCATAATCAG CCATACCACA
  301 TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT
  361 AAAATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA
  421 AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT
  481 TTGTCCAAAC TCATCAATGT ATCTTAACGC GTCGATCATA TTCAATAACC CTTAATATAA
  541 CTTCGTATAA TGTATGCTAT ACGAAGTTAT TAGGTCTGAA GAGGAGTTTA CGTCCAGCCA
  601 AGCTTAGGAT CTCGACCTCG AAATTCTACC GGGTAGGGGA GGCGCTTTTC CCAAGGCAGT
  661 CTGGAGCATG CGCTTTANCA GCCCCGCTGG GCACTTGGCG CTACACAAGT GGCCTCTGGC
  721 CTCGCACACA TTCNACATCC ACCGGTANGC GCCAACCGAC TCCGTTCTTT GGTGGNCCCT
  781 TCGCGCCACC TTCTACTCCT CCCCTAGTCA NGAANTTCCC CCCCCGCCCC GCANCTCGCG
  841 NCGNGCANGN NGTGACAAAN GGAANTANCA CGNCNCACTA NNCTCGTGCA NATGGNNAGC
901 ACNNCNNNNC NNNGGAANCG GGNNNNNNNT TNNGNNNANC NNNNNNNNNN
//
Primer: SV40pA3element Reverse (Pr0108)
Region: lac pro,T3 pro,insert 3'(loxP,mouse PGK ter)
Sequence file: RDB16818_A8Lpe.seq check
>16818_16818_B3C8F_SV40pA3'element Reverse_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNGGNNNNNN NNNNATGNNN NNNNCCNGCA ACGCGGCCTT TTTACGGTTC
   61 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG
  121 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG
  181 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC
  241 GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC
  301 AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC
  361 TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA
  421 AACAGCTATG ACCATGATTA CGCCAAGCGC GCAATTAACC CTCACTAAAG GGAACAAAAG
  481 CTGGGTACCG GATCCTAGTG AACCTCTTCG AGGGACCTAA TAACTTCGTA TAGCATACAT
  541 TATACGAAGT TATATTAAGG GTTCCGGATC TCGACCAGCT TCTGATGGAA TTAGAACTTG
  601 GCAAAACAAT ACTGAGAATG AAGTGTATGT GGAACAGAGG CTGCTGATCT CGTTCTTCAG
  661 GCTATGAAAC TGACACATTT GGAAACCACA GTACTTAGAA CCACAAAGTG GGAATCAAGA
  721 GAAAAACAAT GATCCCACGA GAGATCTATA GATCTATAGA TCATGANTGG GAGGAATGAG
  781 CTGGNCCTTA ATTTGGTTTT GCTTGTTTNA ATTATGATAT CCAACTATGA NNCATTATCA
  841 TAAAGCAATA GTAAAGAGCC TTCAGNNAAA GAGCANGNAT TTATCTAATC CCNCCCNNCC
  901 CCCNCCCCCG TAGCTNCNAT CCTTNNNTNC AAAANGTNNN NNCTCNGNNN NNNCNCNTCT
  961 TNNCNNNTAT GANNNNAAAA ACNTNNNNTT TTNNNNNNNT NNTTATNNNT TNNNNNNNNN
1021 N
//
Primer: PGKpro_F (Pr0249)
Region: insert mid(BSD,mouse PGK ter)
Sequence file: RDB16818_A8Lpf.seq check
>D06157B5_A8LP_2_PGKpro_F_E05_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTCC NGGCCTTTCG ACCTGCATCC NTCTAGATCT
   61 CGAGCCACCA TGGCCAAGCC TTTGTCTCAA GAAGAATCCA CCCTCATTGA AAGAGCAACG
  121 GCTACAATCA ACAGCATCCC CATCTCTGAA GACTACAGCG TCGCCAGCGC AGCTCTCTCT
  181 AGCGACGGCC GCATCTTCAC TGGTGTCAAT GTATATCATT TTACTGGGGG ACCTTGTGCA
  241 GAACTCGTGG TGCTGGGCAC TGCTGCTGCT GCGGCAGCTG GCAACCTGAC TTGTATCGTC
  301 GCGATCGGAA ATGAGAACAG GGGCATCTTG AGCCCCTGCG GACGGTGCCG ACAGGTGCTT
  361 CTCGATCTGC ATCCTGGGAT CAAAGCCATA GTGAAGGACA GTGATGGACA GCCGACGGCA
  421 GTTGGGATTC GTGAATTGCT GCCCTCTGGT TATGTGTGGG AGGGCTAATC GATGATATCA
  481 GATCCCCGGG ATGCAGAAAT TGATGATCTA TTAAACAATA AAGATGTCCA CTAAAATGGA
  541 AGTTTTTCCT GTCATACTTT GTTAAGAAGG GTGAGAACAG AGTACCTACA TTTTGAATGG
  601 AAGGATTGGA GCTACGGGGG TGGGGGTGGG GTGGGATTAG ATAAATGCCT GCTCTTTACT
  661 GAAGGCTCTT TACTATTGCT TTATGATAAT GTTTCATAGT TGGATATCAT AATTTAAACA
  721 AGCAAAACCA AATTAAGGGC CAGCTCATTC CTCCCACTCA TGATCTATAG ATCTATAGAT
  781 CTCTCGTGGG ATCATTGTTT TTCTCTTGAT TCCNACTTTG TGGTTCTAAG TACTGTGGTT
  841 NNNAAATGTG TCAGTTTCAT AGCCTGAAGA ANGAGATCAG CAGCNTCTGT TCCACATACN
  901 CTNCATTCTC ANNATTGNTT TNGNCNNGNT NCTANNTNNN TCNNNNNNTN GNNNNNNNNN
961 N
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]