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pMK390(Stag-3FLAG-BSD)

A plasmid for construction of C-terminal Stag-3FLAG-BSD tagging donors.

Catalog number RDB16818
Resource name pMK390(Stag-3FLAG-BSD)
Clone info. A plasmid for construction of C-terminal Stag-3FLAG-BSD tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Depositor Kanemaki, Masato |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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RDB16818 pMK390(Stag-3FLAG-BSD) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16818_A8Lpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 RDB16818_A8Lpa.seq
>D06157B5_A8LP_2_pUC-M13_-100_C05_08_ABI24.ab1
    1 GAACGGTCAG GCGGATTAGT TGGGTACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC
   61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGAGC TCGGATCCGG
  121 TGCAGGCGCC AAGGAAACCG CCGCTGCAAA ATTCGAACGC CAGCACATGG ACAGCCGATC
  181 CCGGGCTGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA
  241 TGACGATGAC AAGTAGTGTA CAAGTAATCT AGATAACTGA TCATAATCAG CCATACCACA
  301 TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT
  361 AAAATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA
  421 AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT
  481 TTGTCCAAAC TCATCAATGT ATCTTAACGC GTCGATCATA TTCAATAACC CTTAATATAA
  541 CTTCGTATAA TGTATGCTAT ACGAAGTTAT TAGGTCTGAA GAGGAGTTTA CGTCCAGCCA
  601 AGCTTAGGAT CTCGACCTCG AAATTCTACC GGGTAGGGGA GGCGCTTTTC CCAAGGCAGT
  661 CTGGAGCATG CGCTTTAGCA GCCCCGCTGG GCACTTGGCG CTACACAAGT GGCCTCTGGC
  721 CTCGCACACA TTCCACATCC ACCGGTAGGC GCCAACCGAC TCCGTTCTTT GGTGGCCCCT
  781 TCGCGCCACC TTCTACTCCT CCCCTAGTCA GGAAGTTCCC CCCCCGCCCC GCAGCTCGCG
  841 TCGTGCAGGA CGTGACAAAT GGAAGTAGCA CGTCTCACTA GTCTCGTGCA GATGGACAGC
  901 ACCGCTGAGC AATGGAAGCG GGTAGGCCTT TGGGGGCAGC GGCCCAATAG CAGCTTTGCT
  961 CCCTTCGCTT TCTGGGGCTC AGAGGCTGGG AAAGGGTGGT CCGGGGGCGG GCTCAGGCGG
 1021 CTCAGGGCGG GGCGGCGCCG ATGTTCTCGG AGCTAGCATC TGCACGCATC AAAGCGCACG
1081 GATTCTTGAC TC
//
Primer: reverse RDB16818_A8Lpb.seq
>D06157B5_A8LP_2_reverse_D05_11_ABI24.ab1
    1 AAAACCCATT AACCCCTCAC TAAAGGGAAC AAAAGCTGGG TACCGGATCC TAGTGAACCT
   61 CTTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC GAAGTTATAT TAAGGGTTCC
  121 GGATCTCGAC CAGCTTCTGA TGGAATTAGA ACTTGGCAAA ACAATACTGA GAATGAAGTG
  181 TATGTGGAAC AGAGGCTGCT GATCTCGTTC TTCAGGCTAT GAAACTGACA CATTTGGAAA
  241 CCACAGTACT TAGAACCACA AAGTGGGAAT CAAGAGAAAA ACAATGATCC CACGAGAGAT
  301 CTATAGATCT ATAGATCATG AGTGGGAGGA ATGAGCTGGC CCTTAATTTG GTTTTGCTTG
  361 TTTAAATTAT GATATCCAAC TATGAAACAT TATCATAAAG CAATAGTAAA GAGCCTTCAG
  421 TAAAGAGCAG GCATTTATCT AATCCCACCC CACCCCCACC CCCGTAGCTC CAATCCTTCC
  481 ATTCAAAATG TAGGTACTCT GTTCTCACCC TTCTTAACAA AGTATGACAG GAAAAACTTC
  541 CATTTTAGTG GACATCTTTA TTGTTTAATA GATCATCAAT TTCTGCATCC CGGGGATCTG
  601 ATATCATCGA TTAGCCCTCC CACACATAAC CAGAGGGCAG CAATTCACGA ATCCCAACTG
  661 CCGTCGGCTG TCCATCACTG TCCTTCACTA TGGCTTTGAT CCCAGGATGC AGATCGAGAA
  721 GCACCTGTCG GCACCGTCCG CAGGGGCTCA AGATGCCCCT GTTCTCATTT CCGATCGCGA
  781 CGATACAAGT CAGGTTGCCA GCTGCCGCAG CAGCAGCAGT GCCCAGCACC ACGAGTTCTG
  841 CACAAGGTCC CCCCAGTAAA ATGATATACA TTGACACCAG TGAAGATGCG GCCGTCGCTA
  901 GAGAGAGCTG CGCTGGCGAC GCTGTAGTCT TCAGAGATGG GGGATGCTGT TGATTGTAGC
  961 CGTTGCTCTT TCAATGAAGG GTGGGATTTC TTTCTTTGAG ACAAAGGCCT TGGCCATGGA
 1021 TGGCTCGGAG ATCTAGATGG GAATGCCAGT TCGTAAGGGT CCGGAGATGA GGAAGAGGAA
1081 GAACAGCGCC GTCAGAACGG ATGACGGC
//
Primer: PGKpro_F RDB16818_A8Lpc.seq
>D06157B5_A8LP_2_PGKpro_F_E05_14_ABI24.ab1
    1 AGTGGGGGCC GATCTCTCTT CCTCATCTCC GGGCCTTTCG ACCTGCATCC ATCTAGATCT
   61 CGAGCCACCA TGGCCAAGCC TTTGTCTCAA GAAGAATCCA CCCTCATTGA AAGAGCAACG
  121 GCTACAATCA ACAGCATCCC CATCTCTGAA GACTACAGCG TCGCCAGCGC AGCTCTCTCT
  181 AGCGACGGCC GCATCTTCAC TGGTGTCAAT GTATATCATT TTACTGGGGG ACCTTGTGCA
  241 GAACTCGTGG TGCTGGGCAC TGCTGCTGCT GCGGCAGCTG GCAACCTGAC TTGTATCGTC
  301 GCGATCGGAA ATGAGAACAG GGGCATCTTG AGCCCCTGCG GACGGTGCCG ACAGGTGCTT
  361 CTCGATCTGC ATCCTGGGAT CAAAGCCATA GTGAAGGACA GTGATGGACA GCCGACGGCA
  421 GTTGGGATTC GTGAATTGCT GCCCTCTGGT TATGTGTGGG AGGGCTAATC GATGATATCA
  481 GATCCCCGGG ATGCAGAAAT TGATGATCTA TTAAACAATA AAGATGTCCA CTAAAATGGA
  541 AGTTTTTCCT GTCATACTTT GTTAAGAAGG GTGAGAACAG AGTACCTACA TTTTGAATGG
  601 AAGGATTGGA GCTACGGGGG TGGGGGTGGG GTGGGATTAG ATAAATGCCT GCTCTTTACT
  661 GAAGGCTCTT TACTATTGCT TTATGATAAT GTTTCATAGT TGGATATCAT AATTTAAACA
  721 AGCAAAACCA AATTAAGGGC CAGCTCATTC CTCCCACTCA TGATCTATAG ATCTATAGAT
  781 CTCTCGTGGG ATCATTGTTT TTCTCTTGAT TCCCACTTTG TGGTTCTAAG TACTGTGGTT
  841 TCCAAATGTG TCAGTTTCAT AGCCTGAAGA ACGAGATCAG CAGCCTCTGT TCCACATACA
  901 CTTCATTCTC AGTATTGTTT TTGCCAAGTT TCTAATTCCA TCAGAAGCTG GGTCGAGATC
  961 CGGAACCCTT TAAATATAAC TTCGTATAAT GTATGCTATA CGAAGTTTAT TAGGTCCCTC
 1021 GAGGAGGGTT TCACTAGGAT CCCGGTACCC AGCCTTTTTG ATTTCCCTTT TAAGTTGGAG
1081 GATAATTTGC ACGCTGGACG TTAATCATGT CACTAGCTTG GATTATCG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

2019.07.12

GNP_filter3_RDBDEP_html_190431.pl