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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAAV-hSyn-OsTIR1(F74G)

Adeno-associated virus (AAV) expression vector of OsTIR1(F74G) and mAID-EGFP-NES marker with hSyn promoter.

Catalog number RDB18339
Resource name pAAV-hSyn-OsTIR1(F74G)
Clone info. Adeno-associated virus (AAV) expression vector of OsTIR1(F74G) and mAID-EGFP-NES marker with hSyn promoter, see Fig. 2A, B and SFig. 4A of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone pAAV-hSyn (plasmid)
Selectable markers Ampicillin
Gene/insert name Oryza sativa OsTIR1 cDNA Arabidopsis thaliana IAA17 cDNA Aequorea victoria GFP (EGFP) cDNA
Depositor|Developer Kanemaki, Masato |
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External Database
Oryza sativa OsTIR1 Arabidopsis thaliana IAA17 Aequorea victoria GFP (EGFP)

        Reference sequence
          
         
        Sequence (full) RDB18339zzk01.seq provided by the depositor

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
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        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB18339 pAAV-hSyn-OsTIR1(F74G) DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Original reference

        original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Auxin Inducible Degron (AID) System (English text)
        Featured content Auxin Inducible Degron (AID) System (Japanese text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB18339_B0Gfp1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: OsTIR1-N_R1
        Region: insert 5'(OsTIR1(F74G),hSyn pro)
        Sequence file: RDB18339_B0Gfa.seq check
        >D07105A7_B0Gf_1_OsTIR1-N_R1_A11_02_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNTT NNNNTCTCTC TGTGCNGGCA GGAAGCTAAA AATGTGTTCG
           61 ACGACCTCTT CAGGAAAGTA TGTCATGGTG GCGATCCTCT CGACTGCGCT CTCAGGCACG
          121 ACACGACTCC TCCGCTGCCC ACCGCAGACT GAGGCAGCGC TGAGTCGCCG GCGCCGCAGC
          181 GCAGATGGTC GCGCCCGTGC CCCCCTATCT CGCGCCTCGC GTGGTGCGGT CCGGCTGGGC
          241 CGGCGGCGGC GCGGACGCGA CCAAGGTGGC CGGGAAGGGG AGTTTGCGGG GGACCGGCGA
          301 GTGACGTCAG CGCGCCTTCA GTGCTGAGGC GGCGGTGGCG CGCGCCGCCA GGCGGGGGCG
          361 AAGGCACTGT CCGCGGTGCT GAAGCTGGCA GTGCGCACGC GCCTCGCCGC ATCCTGTTTC
          421 CCCTCCCCCT CTCTGATAGG GGATGCGCAA TTTGGGGAAT GGGGGTTGGG TGCTTGTCCA
          481 GTGGGTCGGG GTCGGTCGTC AGGTAGGCAC CCCCACCCCG CCTCATCCTG GTCCTAAAAC
          541 CCACTTGCAC TCATACGCAG GGCCCTCTGC AGTCTAGACA CACGCGTGCG GCCGCAGGAA
        601 CCCCTAGTGA TGGAGTTGGC CACTCCCTCT CTGCGCGCTN
        //
        Primer: OsTIR1_R3
        Region: insert mid(OsTIR1(F74G))
        Sequence file: RDB18339_B0Gfb.seq check
        >18339_18339_B1KhF_1_OsTIR1_R3_C01_07_ABI24.ab1
            1 NNNNNNNNNN TTNNNTTCNC CTTNGNNGCN GGCAAAGTTC AGGGNCACCA GGCTGGTACA
           61 TGAGTCTGGG AAGCAAGACA GCCATCTGGG GCCTCTATCC TCCACTTCAT TCTCCTGGAG
          121 GTCCAGCTCC CGCAGCAGCT TACAGTGGGA GGCCACGGCT GCCAGTCCAT CGGTGCTGAA
          181 GCCTTCGCAA GAAATCAGCA CCAGTGCGCG AAACCTAGGG AAGCTCCGAG CCAGCAGCTC
          241 CAGACTTTCG TCAGAGACCA CCATTCGCTT CATGCGCAGT TCCTCCAGTC CATGACAGCC
          301 GCGAGCGGCT GCCTCGATCC AAGGGCCGGC GTATCCTCCC CAATCAGGGG GCACCAGGTT
          361 AAAGTCGGCG CCGTGGGGTT TCCCCTTGAC GGTCAGTGCG CGCACATTTG GAAACCTAGC
          421 GGCGACCCTG CCTGCTCTCA CAGCATAGCA GTTGCCGACG AACACTCCTC TCCGGCTCAG
          481 GCGTTCGATC TCGTACCACA CTTTGCAGAC CAGGCTCACT GTGTTTCTAT CTCTCTGTGC
          541 AGGCAGGAAG CTAAAAATGT GTTCGACGAC CTCTTCAGGA AAGTATGTCA TGGTGNCGAT
          601 CCTCTCGACT GCGCTCTCAN GCACGACACG ACTCCTCCGC TGCCCACCGC AGANTGANGC
          661 AGCGCTGANT CGCCNGCGCC NCANCGCANA NGGNCGCGCC NNNNCCCCCC NNANCTCNNN
        721 NNNNNNN
        //
        Primer: Venus-N_R
        Region: insert mid(EGFP 5',mini-AID,P2A,OsTIR1 3')
        Sequence file: RDB18339_B0Gfc.seq check
        >D07105A7_B0Gf_1_Venus-N_R_D11_11_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NTTGTGGNCG TTNANGTCGC CGTCCNGCTC GACCNGGATG
           61 GGCACCACCC CGGTGAACAG CTCCTCGCCC TTGCTCACCA TGCTAGCTTT ATACATCCTC
          121 AAATCGATTT TCCTCAAGTA CGGTGCTCCG TCCATTGATA CCTTCACGAA CGCCGCCGCC
          181 TCCGGGCCAC CGCTTGATTT TTGGCAGGAA ACCATCACGT TCTTCCGGTA TGATCTCACC
          241 GGTGGCCATC CCACAACTTG TGCCTTGGCC GGAGGTTTGG CTGGATCTTT AGGACAAGCA
          301 CTCTTCTCCT TAGATCTAGG TCCAGGGTTC TCCTCCACGT CTCCAGCCTG CTTCAGCAGG
          361 CTGAAGTTAG TAGCTCCGCT TCCTGATCTC AGAATCTTCA CAAAGTTGGG AGCATCATCT
          421 CTTGCCCCTG CGGTGGTCCG ATACACATAC AGTTTCTCCA CCTTTGGCAG GTCGCCATGA
          481 TTTTCCTCCA TTTCGTTAGA CCCGTCCCGC TCATTAATGA CTTCCACGTT CAGCATAGGC
          541 ATTTTACTAG CCAGGACCTG ACAGCCCTTC AGAGTCACAT TGCAACTAGA CATCCACAGG
          601 CTCCTCATTG TCTCGTATCT GGCAAAGTTT CCCAGCAGAG CGGCATCCCC GAAAGGGCTG
          661 TCCCGGATTT CCAGTTTTCG CAGATTCTTA CACCCGTTCA TCACGTGCAT CATGCCCTTA
          721 TCGGAGTCTC CGGCAAATGC AATGCTCAGC ATCTCCAGCT GCTTAGCATA CTTGCCGATG
          781 TACATGAACA CTTTGTCTGT CAGCAGGCCG GAAATGCTCA GCCTCTGGAG TCCNTTGCAC
          841 TCTCTCACGA TAGCTCCNAA CCCTTCATCC AGNGGCTGGG ANGTGANCAC GTCGGGTTTG
          901 CCNGGTTCCA NAATACACAG CCNAANTNGG GNGAAATTAN GGNANTTCTN GGCGANNNTC
          961 ACCANNGNAG CATTANNCAT CTGANGNNAN AAATACAGCN NNNNNTCAGC TTNGGGNATC
        1021 CNNNNGANAN NNNNNNNNN
        //
        Primer: mVenus-M_F
        Region: insert 3'(EGFP 3',NLS,SV40 pA),WPRE
        Sequence file: RDB18339_B0Gfd.seq check
        >D07105A7_B0Gf_1_mVenus-M_F_B11_05_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC NNGGNNNNNN TCANGANCCG CNCNCAACAT
           61 CGAGGANGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG
          121 CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC
          181 CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT
          241 CGGCATGGAC GAGCTGTACA AGGGTGCAGG CGCCCAAGCT TCGAATTCTG CAGTCGACCT
          301 GCCCCCCCTG GAGCGCCTGA CCCTGATCGA CGGTACCGCG GGCCCGGGAT CCACCGGATC
          361 TAGATAACTG ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA
          421 CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG TTGTTAACTT
          481 GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA
          541 AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTAAAT
          601 TCGATATCAA GCTTATCGAT AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG
          661 GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT
          721 ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGNTTGC
          781 TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGNGGTG TGCACTGTGT
          841 TTGCTGACGC NNCCCCCACT GGTTGGGGCA TTGCCACCAC CNGTCAGCNN CTTTCCNGGA
          901 NTTNNGCTTT CCCCCTNCNT ATTGNCACGG CGNAACTCAT CGNNGCCTGN CTNGNCCNCT
          961 GCNNGNNNNN GGGGCTCNGN NTGTNNGGNA CTNNNNNTTC NNNNNNNNNG NNGGNNAATC
        1021 ATCNNNNNTN NCNNNNNNNN NN
        //
        Primer: f1ori_F
        Region: f1 ori,AAV2 ITR
        Sequence file: RDB18339_B0Gfe.seq check
        >18339_18339_B1KhF_1_f1ori_F_G02_20_ABI24.ab1
            1 NNNNNNNNNN NNNNNGNANN GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC
           61 TTAATGCGCC GCTACAGGGC GCGTACTATG GTTGCTTTGA CGTATGCGGT GTGAAATACC
          121 GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCCCC TGCAGGCAGC TGCGCGCTCG
          181 CTCGCTCACT GAGGCCGCCC GGGCAAAGCC CGGGCGTCGG GCGACCTTTG GTCNCCCGGC
        241 CTCNNNGANN NNNNNNNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522.

        2022.08.12

        GNP_filter3_RDBDEP_html_220812.pl