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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK419 (#RDB18342)

A plasmid for generate yeast lines expressing OsTIR1(F74G) under the GAL promoter.

Alternative name GAL-OsTIR1(F74G)
Clone info. A plasmid for generate yeast lines expressing OsTIR1(F74G) under the GAL promoter, see Fig. 2C and SFig. 4D of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone pBluescriptII KS- (plasmid)
Selectable markers Ampicillin (E. coli), URA3 (S. cerevisiae).
Gene/insert name Oryza sativa OsTIR1 cDNA
Depositor|Developer Kanemaki, Masato |
 
Sequence (full) RDB18342zzk02.seq provided by the depositor

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18342 pMK419 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMK419 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18342).

Reference section:

Yesbolatova, A., Saito, Y., Kitamoto, N., Makino-Itou, H., Ajima, R., Nakano, R., Nakaoka, H., Fukui, K., Gamo, K., Tominari, Y., Takeuchi, H., Saga, Y., Hayashi, K., Kanemaki, M.T., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18342_B0Gfp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-CAPbs_up_F2 (Pr0739)
Region: insert 5'(GAL1-10 pro)
Sequence file: RDB18342_B0Gfa.seq check
>D07105B1_B0Gf_1_pUC-CAPbs_up_F2_E03_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGCTTNNNN CTTTATGCTT CCGGCTCGTA TGTTGTGTGG
   61 AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT
  121 CGAAATTAAC CCTCACTAAA GGGAACAAAA GCTGGTACCG GGCCCCCCCT CGAGGTCGAC
  181 GGTATCGATA AGCTTGATAT CGAATTCGAC AGGTTATCAG CAACAACACA GTCATATCCA
  241 TTCTCAATTA GCTCTACCAC AGTGTGTGAA CCAATGTATC CAGCACCACC TGTAACCAAA
  301 ACAATTTTAG AAGTACTTTC ACTTTGTAAC TGAGCTGTCA TTTATATTGA ATTTTCAAAA
  361 ATTCTTACTT TTTTTTTGGA TGGACGCAAA GAAGTTTAAT AATCATATTA CATGGCATTA
  421 CCACCATATA CATATCCATA TACATATCCA TATCTAATCT TACTTATATG TTGTGGAAAT
  481 GTAAAGAGCC CCATTATCTT AGCCTAAAAA AACCTTCTCT TTGGAACTTT CAGTAATACG
  541 CTTAACTGCT CATTGCTATA TTGAAGTACG GATTAGAAGC CGCCGAGCGG GTGACAGCCC
  601 TCCGAAGGAA GACTCTCCTC CGTGCGTCCT CGTCTTCACC GGTCGCGTTC CTGAAACGCA
  661 GATGTGCCTC GCGCCGCACT GCTCCGAACA ATAAAGATTC TACAATACTA GCTTTTATGG
  721 NTATGAAGAG GAAAAATTGG CAGTAACCTG GCCCCACAAA CCTTCAAATG AACGAATCAA
  781 ATTAACAACC ATAGGANGAN AATGCGATTA GTTTTTTAGN CTTATTTCTG GGGTAATTAA
  841 TCAGCGAANC GATGANTTTT GATCTATTAA CAGATATATA AATGNAAAAA CTGCNTAACC
  901 ACTTTNACNN ATACTTTCAA CATTTTCNNN TTGNANTACT TCTTANTNNA ATGTAATAAA
  961 AGTATCAACN AAAAANTGNT NATATACCTC TATACTTTNN NGNNNNNGAN AAAAAANCCN
1021 GNNNNNNNNA NTANGNNNNN NTTNNNNNNN NNN
//
Primer: pBAC_lac_F (Pr0544)
Region: insert 3'(OsTIR1(F74G) 3')
Sequence file: RDB18342_B0Gfb.seq check
>D07105B1_B0Gf_1_pBAC_lac_F_H07_22_ABI24.ab1
    1 GNNNNNNNNN NNNNNNNNNN NNNNANGANT CNCTATAGGG CGAANTGGAN CTCCNCCGCG
   61 GTGGCGGCCG CTCATAAAAT CTTGACGAAG TTAGGAGCAT CATCTCTGGC ACCTGCGGTT
  121 GTTCTGTAGA CGTATAACTT TTCAACTTTA GGCAAGTCAC CATGGTTTTC TTCCATTTCA
  181 TTGGAACCAT CTCTTTCGTT AATAACTTCA ACGTTCAACA TTGGCATCTT TGAAGCTAAA
  241 ACTTGACAAC CTTTCAAGGT AACGTTACAA CTGGACATCC ACAAAGATCT CATTGTTTCA
  301 TATCTTGCGA AATTACCCAA CAAAGCTGCG TCACCAAATG GAGAATCTCT GATTTCCAAC
  361 TTTCTTAAGT TCTTACAACC GTTCATAACA TGCATCATAC CCTTATCGGA GTCACCAGCG
  421 AATGCGATTG ACAACATTTC CAATTGCTTA GCGTATTTAC CGATGTACAT AAAAACCTTA
  481 TCTGTCAACA AACCACTGAT GGACAATCTT TGCAAACCCT TACACTCTCT AACGATTGCA
  541 CCAAAACCTT CATCTAAAGG TTGTGATGTA ACAACATCAG GTTTACCTGG TTCCAAGATA
  601 CACAATCTGA ATCTAGTGAA GTTTGGACAA TTTTTAGCAA CAGTAACCAA AGCTGCGTTG
  661 GTCATTTGAT GACAGAAGTA CAACAAGGAG TTCAACTTAN GACAACCCAA TGAAACTGCA
  721 ACTAAACCTT CTTCTGTAAC AGCAGANTAA CCTGCAACAT AGAAATCACT TGGAAAAACT
  781 CTCAATTCTT GCAAATCCTT ACAAGAAGAA NCAACAACTT GCNAACCTTT ATCTGAGATA
  841 CAATCCAAAA NCCACAATCN TNGCANCTTA ACACATCTAG AGNTCATCTT TGNCAAATCA
  901 CTAGCATCCA AAGTTGGNGC GTAANANAAA TTCAAANNNG TCNATNGANC ACANNANNNN
961 NATATAAAAN ANNANCNANC NGGNNNTNNN NNCNNAAANN NNNNNNNN
//
Primer: GAL1pro_F (Pr0030)
Region: insert mid(GAL1-10 pro,OsTIR1(F74G) 5')
Sequence file: RDB18342_B0Gfc.seq check
>D07105B1_B0Gf_1_GAL1pro_F2_B08_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNTNNNA TACCTCTATA CTTTAACGTC NAGGAGAAAA
   61 AACCCCGGAT CCACTAGTAT GACTTATTTT CCTGAAGAAG TTGTAGAACA CATATTTTCC
  121 TTTTTACCTG CTCAAAGAGA TAGAAACACC GTCTCCTTGG TCTGTAAGGT TTGGTACGAA
  181 ATCGAAAGAT TGTCTAGAAG AGGTGTTTTT GTTGGTAATT GTTACGCAGT TAGAGCTGGT
  241 AGAGTTGCTG CAAGATTCCC AAACGTTAGA GCATTGACTG TTAAAGGTAA ACCTCATGGT
  301 GCTGATTTCA ATTTGGTTCC ACCTGATTGG GGTGGTTATG CTGGTCCATG GATTGAAGCT
  361 GCAGCTAGAG GTTGTCACGG TTTGGAAGAA TTGAGAATGA AGAGAATGGT TGTTTCTGAT
  421 GAATCATTGG AATTGTTAGC AAGATCTTTT CCTAGATTCA GAGCTTTGGT TTTGATCTCA
  481 TGTGAAGGTT TTTCCACCGA TGGTTTAGCA GCTGTTGCTT CACATTGTAA GTTGTTGAGA
  541 GAATTGGATT TGCAAGAAAA CGAAGTTGAA GATAGAGGTC CAAGATGGTT ATCTTGTTTT
  601 CCTGATTCCT GTACAAGTTT GGTTTCTTTG AACTTCGCAT GTATCAAGGG TGAAGTTAAC
  661 GCTGGTAGTT TGGAAAGATT AGTTTCTAGA TCACCAAATT TGAGATCTTT GAGATTGAAC
  721 AGATCCGTTA GTGTTGATAC CTTGGCAAAG ATCTTGTTGA GAACTCCTAA TTTGGAAGAN
  781 TTGGGTACTG GTAACTTGAC AGATGATTTC CAAACAGAAT CATACTTCAA GTTGACCTCC
  841 GCTTTGGAAA ANTGTAAGAT GTTGAGATCT TTGTCAGGNT TTTGGGATGN AAGTNCAGTT
  901 TGTTTGTCTT TTATATATCC TTTGTGTGCT CNATTGACTG NNTTGAATTT GNCTTACGCA
961 CNNNCTTTNN ATGCTANNGA NTTGANNAAG NNGATCTCTN NNNNNNNNN
//
Primer: URA3pro_F (Pr0170)
Region: URA3 pro, URA3 5'
Sequence file: RDB18342_B0Gfd.seq check
>D07105B1_B0Gf_1_URA3pro_F_H03_24_ABI24.ab1
    1 NNNNNNNNNN NNNNNNANAN NNTGNNNTTG CCNNGTATTC TTAACCCNAC TGCACAGAAC
   61 AAAAACCTGC AGGAAACGAA GATAAATCAT GTCGAAAGCT ACATATAAGG AACGTGCTGC
  121 TACTCATCCT AGTCCTGTTG CTGCCAAGCT ATTTAATATC ATGCACGAAA AGCAAACAAA
  181 CTTGTGTGCT TCATTGGATG TTCGTACCAC CAAGGAATTA CTGGAGTTAG TTGAAGCATT
  241 AGGTCCCAAA ATTTGTTTAC TAAAAACACA TGTGGATATC TTGACTGATT TTTCCATGGA
  301 GGGCACAGTT AAGCCGCTAA AGGCATTATC CGCCAAGTAC AATTTTTTAC TCTTCGAAGA
  361 CAGAAAATTT GCTGACATTG GTAATACAGT CAAATTGCAG TACTCTGCGG GTGTATACAG
  421 AATAGCAGAA TGGGCAGACA TTACGAATGC ACACGGTGTG GTGGGCCCAG GTATTGTTAG
  481 CGGTTTGAAG CAGGCGGCAG AAGAAGTAAC AAAGGAACCT AGAGGCCTTT TGATGTTAGC
  541 AGAATTGTCA TGCAAGGGCT CCCTATCTAC TGGAGAATAT ACTAAGGGTA CTGTTGACAT
  601 TGCGAAGAGC GACAAAGATT TTGTTATCGG CTTTATTGCT CAAAGAGACA TGGGTGGAAG
  661 AGATGAAGGT TACGATTGGT TGATTATGAC ACCCGGTGTG GGTTTAGATG ACAAGGGAGA
  721 CGCATTGGGT CAACAGTATA GAACCGTGGA TGANGTGGTC TCTACAGGAT CTGACATTAT
  781 TATTGNTGGA AGAGGANTAT TTGCAAAGGG AAGGGANGCT AANGTAGAGG GTGAANGTTA
  841 CAGAAAANCA NGCTGGGAAG CATATTTGAG AAGANGCGGC CAGCAAAACT NAAAAACTGT
  901 ATTATAAGTN AATGNATGTA TACTNAACTC NCAAATTANA GCTTCNNNTT NATTATATCA
  961 GTTATTANCC NATGCNGTGT GNAANANCNN NNNNANGCNN NNNNNNAAAA NNNNNNCNNC
1021 NNNAANTNGN ANNCNNNNNN NTTNGNNNAA NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]