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pMK419 (#RDB18342)

A plasmid for generate yeast lines expressing OsTIR1(F74G) under the GAL promoter.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Yesbolatova, A., Nat. Commun. 11: 5701 (2020). PMID 33177522. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name GAL-OsTIR1(F74G)
Clone info. A plasmid for generate yeast lines expressing OsTIR1(F74G) under the GAL promoter, see Fig. 2C and SFig. 4D of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone pBluescriptII KS- (plasmid)
Selectable markers Ampicillin (E. coli), URA3 (S. cerevisiae).
Growth conditions LB+Amp 37oC
Gene/insert name Oryza sativa OsTIR1 cDNA
Depositor|Developer Kanemaki, Masato |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
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必要書類
提供依頼書  [依頼書の記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。

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RDB18342 pMK419 DNA solution

How to cite this biological resource

Materials & Methods section:

The pMK419 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18342).

Reference section:

Yesbolatova, A., Saito, Y., Kitamoto, N., Makino-Itou, H., Ajima, R., Nakano, R., Nakaoka, H., Fukui, K., Gamo, K., Tominari, Y., Takeuchi, H., Saga, Y., Hayashi, K., Kanemaki, M.T., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [PubMed] [Article] [RRC of NBRP]