A plasmid for construction of C-terminal mAID-mClover tagging donors.
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External Database Arabidopsis thaliana AtIAA17 Aequorea victoria GFP Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
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Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ![]() This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]). |
提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。 |
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提供依頼 | 手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word ![]() 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word ![]() GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB13932 | pMK289 (mAID-mClover-NeoR) | DNA solution |
Electronic file
Original reference
original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. |
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Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Auxin Inducible Degron (AID) System (English text) |
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Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13932_A6B8p1_v2.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: M13_-40 Region: insert5'(T7pro,Linker,mAID,mClover(A206K)) Sequence file: RDB13932_A6B8e.seq ![]() |
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>D03070A16_A6B8_1_M13(-40)_A05_02.ab1 1 AAGGCAGCTA CGCGCGTATA CGACTCACTA TAGGGCGAAT TGGAGCTCCC CGGGATCCGG 61 TGCAGGCGCC AAGGAGAAGA GTGCTTGTCC TAAAGATCCA GCCAAACCTC CGGCCAAGGC 121 ACAAGTTGTG GGATGGCCAC CGGTGAGATC ATACCGGAAG AACGTGATGG TTTCCTGCCA 181 AAAATCAAGC GGTGGCCCGG AGGCGGCGGC GTTCGTGAAG GTATCAATGG ACGGAGCACC 241 GTACTTGAGG AAAATCGATT TGAGGATGTA TAAAGCTAGC ATGGTGAGCA AGGGCGAGGA 301 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA 361 GTTCAGCGTC CGCGGCGAGG GCGAGGGCGA TGCCACCAAC GGCAAGCTGA CCCTGAAGTT 421 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCTTCGGCTA 481 CGGCGTGGCC TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC 541 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTCT TTCAAGGACG ACGGTACCTA 601 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA 661 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTTCAA 721 CAGCCACAAC GTCTATATCA CGGCCGACAA GCAGAAGAAC GGCATCAAGG CTAACTTCAA 781 GATCCGCCAC AACGTTGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC 841 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCC ATCAGTCCAA 901 GCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC 961 CGCCCGGGAT TACACATGGC ATGGAACGAG CTGTACAAGT AATCTAGATA ACTGATCATA 1021 TCAGCCCATA CCACATTTGT AGAGGTTTTA CTGCTTAAAA CCTCCCACAC TCCCCTTGAA 1081 CTTGAAACAT AAAATGAATG CCATGATGGT GCTACCTGTC TATGCGACTT AAAATGGTCA 1141 TAGCCATAGC CATTCACGAT // |
Primer: SV40polyA-R Region: insert mid(mClover(A206K)) Sequence file: RDB13932_A6B8f.seq ![]() |
>D03070A16_A6B8_1_SV40polyA-R_H04_22.ab1 1 TCCCCCCCCC CGGTATTTAG CAGTAACCTC TACAATGTTG GTATGGGCTG ATTATGATCA 61 GTTATCTAGA TTACTTGTAC AGCTCGTCCA TGCCATGTGT AATCCCGGCG GCGGTCACGA 121 ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGC TTGGACTGAT 181 GGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT 241 GGTAGTGGTC GGCGAGCTGC ACGCTGCCGT CCTCAACGTT GTGGCGGATC TTGAAGTTAG 301 CCTTGATGCC GTTCTTCTGC TTGTCGGCCG TGATATAGAC GTTGTGGCTG TTGAAGTTGT 361 ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCTCGA 421 TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGGTACCGT 481 CGTCCTTGAA AGAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG GACTTGAAGA 541 AGTCGTGCTG CTTCATGTGG TCGGGGTACC GGCTGAAGCA GGCCACGCCG TAGCCGAAGG 601 TGGTCACGAG GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT 661 CAGCTTGCCG TTG // |
Primer: SV40pro_F_V2 Region: insert mid(NeoR_KanR) Sequence file: RDB13932_A6B8g.seq ![]() |
>D03070A16_A6B8_1_SV40pro_F_V2_E10_13.ab1 1 GGGGCAGCTT TTGGAGGCCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG 61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG 601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT 721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 961 GAATCGTTTT CCGGGACGCC CGGCTGGATG ATCCCTCCAG CGCGGGGATC TCATGCTGGG 1021 AGTTCTTTCG CCCACCCTAG GGGAGGCTAA CTGAAACACG GAAGAGACAA TACCGGAAAG 1081 ACCGCGCTAT GACGGCCAAT AAAAGGACAG GAAATAAAAC CGCCCACCG // |
Primer: M13R Region: insert3'(TKp(A),NeoR_KanR) Sequence file: RDB13932_A6B8h.seq ![]() |
>D03070A16_A6B8_1_M13R_A04_01.ab1 1 CTGGGCGCAA TTAACCTCAC TAAAGGGAAC AAAAGCTGGG TACCGGATCC AACGACCCAA 61 CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG 121 TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT 181 CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT 241 CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG 301 TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT 361 GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC 421 GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GGTCCGCCAC 481 ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG 541 CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG 601 CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC 661 GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC 721 GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA 781 TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA 841 TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC 901 CCGTCGTGGC CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAAGGCACCG 961 GGACAGGGTC GGGTCTTTGA CAAAAAGAAT CCGGGGCGGC CCCTGCGCTT GACAGGCCGG 1021 GAACCACGGG CGGGCAATCA GAAGCAGCCC GAATTGTCTG TTGGTGGCCC AGTCAATAGC 1081 CGGAAATAGG CTCTCCACCC CAAGCCGGGC CGGGAGGAAC TGCGTGCCAA TTCCAATCCT 1141 TTGGCTCATT CAATGCCGGA AAACGGAATT CCCTTCACAT CACCTCTG // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. |
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reference | Goto, H., Chk1-mediated Cdc25A degradation as a critical mechanism for normal cell cycle progression. J Cell Sci. 2019 Jan 25;132(2). pii: jcs223123 (2019). PMID 30635443. |
reference | Devrekanli, A., Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes. Methods in Molecular Biology 1369: 257-278 (2016). PMID 26519318. |
reference | Takagi, M., Perichromosomal protein Ki67 supports mitotic chromosome architecture. Genes Cells 21 (10): 1113-1124 (2016). PMID 27610954. |
reference | Nishimura, K., Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Curr. Protoc. Cell Biol. \64: 20.9.1-16 (2014). PMID 25181302. |
2022.05.17
GNP_filter3_RDBDEP_html_220516.pl