A plasmid for construction of C-terminal mAID-mClover tagging donors.
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External Database Arabidopsis thaliana AtIAA17 Aequorea victoria GFP Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
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Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ![]() |
提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。 |
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提供依頼 | 手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word ![]() 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word ![]() |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB13932 | pMK289 (mAID-mClover-NeoR) | DNA solution |
Materials & Methods section:
The pMK289 (mAID-mClover-NeoR) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13932). |
Reference section:
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Auxin Inducible Degron (AID) System (English text) |
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Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13932_A6B8p1-4.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: M13_-40 (Pr0410) Region: T7 pro,insert 5'(Linker,mAID, mClover 5') Sequence file: RDB13932_A6B8i.seq ![]() |
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>D03070A16_A6B8_1_M13(-40)_A05_02.ab1 1 NNNNNNNNNN NNCNCNNANN NGNNTNNCNN TAGGGCGAAT TGGAGCTCCC CGGGNTCCGG 61 TGCAGGCGCC AAGGAGAAGA GTGCTTGTCC TAAAGATCCA GCCAAACCTC CGGCCAAGGC 121 ACAAGTTGTG GGATGGCCAC CGGTGAGATC ATACCGGAAG AACGTGATGG TTTCCTGCCA 181 AAAATCAAGC GGTGGCCCGG AGGCGGCGGC GTTCGTGAAG GTATCAATGG ACGGAGCACC 241 GTACTTGAGG AAAATCGATT TGAGGATGTA TAAAGCTAGC ATGGTGAGCA AGGGCGAGGA 301 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA 361 GTTCAGCGTC CGCGGCGAGG GCGAGGGCGA TGCCACCAAC GGCAAGCTGA CCCTGAAGTT 421 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCTTCGGCTA 481 CGGCGTGGCC TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC 541 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTCT TTCAAGGACG ACGGTACCTA 601 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA 661 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTTCAA 721 CAGCCACAAC GTCTATATCA CGGCCGACAA GCAGAAGAAC GGCATCAANG CTAACTTCAA 781 GATCCGCCAC AACGTTGANG ANGGNAGCGN GCANCTCGCC GACCACTACC AGCAGANCAC 841 CCCCATCGGC GACGGNCCCG TGCTGCTGNC CGANNACCAC TACCTNANCC ATCAGNCCAA 901 GCTGANCAAA GANCCNNACG AGAANCGCGA TNNCNTGGNC CNGCTNNNNN NNNNGANNNN 961 NNNCNNGNNN NNNNNNTGGN NNNNNNNNAN NNNNNNNNN // |
Primer: M13R (Pr0160) Region: insert 3'(NeoR_KanR 3') Sequence file: RDB13932_A6B8j.seq ![]() |
>D03070A16_A6B8_1_M13R_A04_01.ab1 1 NNNNNNNNNN NNNNNNNCNN NNNNGGGAAN NNAAGCTGGG TACCGGATCC NACGACCCAA 61 CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG 121 TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT 181 CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT 241 CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG 301 TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT 361 GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC 421 GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GGTCCGCCAC 481 ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG 541 CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG 601 CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC 661 GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC 721 GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA 781 TACTTTCTCG GCAGGAGCAA NGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA 841 TAGCAGCNAG TNCCTTCCCG CTTCANTGAC AACGTCGAGC ACAGCTGCNC NNNGAACGCC 901 CCGTCNNNGN CNAGCNNCNA NAGCCGCGCN GCCNCGTCTT GCAGTTNATT CNNGGNNNCN 961 GNACANGGTC NGNNNNTNGN NAAAAANNAN NNNGGGNNNC CCCNNCNNNN NANNNNCNNG 1021 NNANNANNNN CNGNCNNNNA NNNNNNNNNN // |
Primer: SV40polyA-R (Pr0109) Region: insert mid(SV40 pA,mClover 3') Sequence file: RDB13932_A6B8k.seq ![]() |
>D03070A16_A6B8_1_SV40polyA-R_H04_22.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNCCTC NNNNNNGNNG GNNNNNGCTG ATTANGNTNN 61 NTTATCTAGA TTACTTGNNN NNNTCGTCCA TGCCATGTGT AATCCCGGCG GCGGTCACNA 121 ACTCCANCNN GACCATGTGA TCGCGCTTCT CGNNNNNGTC TTTGCTCANC TTGGACTGAT 181 GGCTCANGTA GNGNNNGTCG GGCAGCANCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT 241 GGTAGTGGTC GGCNAGCTGC ACNCTGCCGT CCTCAACGTT GTGGCGGATC TTGNNNNTNN 301 CCTTGATGCC GTTCTTCTGC TTGTNNNNNN TGATATAGAC NTTGNGNCTG TNNANNNTGT 361 ACTCCANCTT GTGCCCCANG NNNNNNCCGT CCTCCNNNNN NTCNATGCCC TTCAGCTCGA 421 TGCGGTNNNN NNNNGTGTNN NCNTCNAACT NNNNNNNNGC NCGNNNNNNG TANGTNCCGT 481 CNTCNTNNAN AGANANNNNN NNNNN // |
Primer: SV40pro_F_V2 (Pr0154) Region: insert mid(NeoR_KanR5 ') Sequence file: RDB13932_A6B8l.seq ![]() |
>D03070A16_A6B8_1_SV40pro_F_V2_E10_13.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGCTTTNGN NNNNNCGATC NAGAGNNAGG NNGAGGNNCG 61 TTTCGCATGA TTGAACNAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG 601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT 721 CANGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC ANCGCATCGC CTTCTATCGN 841 CTTCTTGACG ANTTCTTCTG ANCGGGANTC TGGGGNTCGA AATGNNCGNN CNNNCGACGC 901 CCAACCNGNC NTCACGANAN TNCGANNCCN CCGCCGNNTT CTATGAAAGN NNNGNNTNNN 961 NAATCGTTTT NNGGGANNNN NNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. |
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reference | Goto, H., Chk1-mediated Cdc25A degradation as a critical mechanism for normal cell cycle progression. J Cell Sci. 2019 Jan 25;132(2). pii: jcs223123 (2019). PMID 30635443. |
reference | Devrekanli, A., Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes. Methods in Molecular Biology 1369: 257-278 (2016). PMID 26519318. |
reference | Takagi, M., Perichromosomal protein Ki67 supports mitotic chromosome architecture. Genes Cells 21 (10): 1113-1124 (2016). PMID 27610954. |
reference | Nishimura, K., Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Curr. Protoc. Cell Biol. \64: 20.9.1-16 (2014). PMID 25181302. |
2023.05.01
GNP_filter3_RDBDEP_html_230501.pl