Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK289 (mAID-mClover-NeoR)

A plasmid for construction of C-terminal mAID-mClover tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

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Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13932_A6B8p1-4.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410) Region: T7 pro,insert 5'(Linker,mAID, mClover 5') Sequence file: RDB13932_A6B8i.seq
>D03070A16_A6B8_1_M13(-40)_A05_02.ab1     1 NNNNNNNNNN NNCNCNNANN NGNNTNNCNN TAGGGCGAAT TGGAGCTCCC CGGGNTCCGG    61 TGCAGGCGCC AAGGAGAAGA GTGCTTGTCC TAAAGATCCA GCCAAACCTC CGGCCAAGGC   121 ACAAGTTGTG GGATGGCCAC CGGTGAGATC ATACCGGAAG AACGTGATGG TTTCCTGCCA   181 AAAATCAAGC GGTGGCCCGG AGGCGGCGGC GTTCGTGAAG GTATCAATGG ACGGAGCACC   241 GTACTTGAGG AAAATCGATT TGAGGATGTA TAAAGCTAGC ATGGTGAGCA AGGGCGAGGA   301 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA   361 GTTCAGCGTC CGCGGCGAGG GCGAGGGCGA TGCCACCAAC GGCAAGCTGA CCCTGAAGTT   421 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCTTCGGCTA   481 CGGCGTGGCC TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC   541 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTCT TTCAAGGACG ACGGTACCTA   601 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA   661 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTTCAA   721 CAGCCACAAC GTCTATATCA CGGCCGACAA GCAGAAGAAC GGCATCAANG CTAACTTCAA   781 GATCCGCCAC AACGTTGANG ANGGNAGCGN GCANCTCGCC GACCACTACC AGCAGANCAC   841 CCCCATCGGC GACGGNCCCG TGCTGCTGNC CGANNACCAC TACCTNANCC ATCAGNCCAA   901 GCTGANCAAA GANCCNNACG AGAANCGCGA TNNCNTGGNC CNGCTNNNNN NNNNGANNNN 961 NNNCNNGNNN NNNNNNTGGN NNNNNNNNAN NNNNNNNNN //
Primer: M13R (Pr0160) Region: insert 3'(NeoR_KanR 3') Sequence file: RDB13932_A6B8j.seq
>D03070A16_A6B8_1_M13R_A04_01.ab1     1 NNNNNNNNNN NNNNNNNCNN NNNNGGGAAN NNAAGCTGGG TACCGGATCC NACGACCCAA    61 CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG   121 TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT   181 CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT   241 CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG   301 TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT   361 GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC   421 GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GGTCCGCCAC   481 ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG   541 CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG   601 CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC   661 GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC   721 GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA   781 TACTTTCTCG GCAGGAGCAA NGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA   841 TAGCAGCNAG TNCCTTCCCG CTTCANTGAC AACGTCGAGC ACAGCTGCNC NNNGAACGCC   901 CCGTCNNNGN CNAGCNNCNA NAGCCGCGCN GCCNCGTCTT GCAGTTNATT CNNGGNNNCN   961 GNACANGGTC NGNNNNTNGN NAAAAANNAN NNNGGGNNNC CCCNNCNNNN NANNNNCNNG 1021 NNANNANNNN CNGNCNNNNA NNNNNNNNNN //
Primer: SV40polyA-R (Pr0109) Region: insert mid(SV40 pA,mClover 3') Sequence file: RDB13932_A6B8k.seq
>D03070A16_A6B8_1_SV40polyA-R_H04_22.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNCCTC NNNNNNGNNG GNNNNNGCTG ATTANGNTNN    61 NTTATCTAGA TTACTTGNNN NNNTCGTCCA TGCCATGTGT AATCCCGGCG GCGGTCACNA   121 ACTCCANCNN GACCATGTGA TCGCGCTTCT CGNNNNNGTC TTTGCTCANC TTGGACTGAT   181 GGCTCANGTA GNGNNNGTCG GGCAGCANCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT   241 GGTAGTGGTC GGCNAGCTGC ACNCTGCCGT CCTCAACGTT GTGGCGGATC TTGNNNNTNN   301 CCTTGATGCC GTTCTTCTGC TTGTNNNNNN TGATATAGAC NTTGNGNCTG TNNANNNTGT   361 ACTCCANCTT GTGCCCCANG NNNNNNCCGT CCTCCNNNNN NTCNATGCCC TTCAGCTCGA   421 TGCGGTNNNN NNNNGTGTNN NCNTCNAACT NNNNNNNNGC NCGNNNNNNG TANGTNCCGT 481 CNTCNTNNAN AGANANNNNN NNNNN //
Primer: SV40pro_F_V2 (Pr0154) Region: insert mid(NeoR_KanR5 ') Sequence file: RDB13932_A6B8l.seq
>D03070A16_A6B8_1_SV40pro_F_V2_E10_13.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGCTTTNGN NNNNNCGATC NAGAGNNAGG NNGAGGNNCG    61 TTTCGCATGA TTGAACNAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG   121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG   181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT   241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA   301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG   361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT   421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA   481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG   541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG   601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG   661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT   721 CANGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC   781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC ANCGCATCGC CTTCTATCGN   841 CTTCTTGACG ANTTCTTCTG ANCGGGANTC TGGGGNTCGA AATGNNCGNN CNNNCGACGC   901 CCAACCNGNC NTCACGANAN TNCGANNCCN CCGCCGNNTT CTATGAAAGN NNNGNNTNNN 961 NAATCGTTTT NNGGGANNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl