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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK425

A plasmid for generate yeast lines expressing OsTIR1(F74A) under the GAL promoter.

Catalog number RDB18343
Resource name pMK425
Alternative name GAL-OsTIR1(F74A)
Clone info. A plasmid for generate yeast lines expressing OsTIR1(F74A) under the GAL promoter, see Fig. 2C and SFig. 4D of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone pBluescriptII KS- (plasmid)
Selectable markers Ampicillin (E. coli), URA3 (S. cerevisiae).
Gene/insert name Oryza sativa OsTIR1 cDNA
Depositor|Developer Kanemaki, Masato |
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External Database
Oryza sativa OsTIR1

          Reference sequence
            
           
          Sequence (full) RDB18343zzk02.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
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          RDB18343 pMK425 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pMK425 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18343).

          Reference section:

          Yesbolatova, A., Saito, Y., Kitamoto, N., Makino-Itou, H., Ajima, R., Nakano, R., Nakaoka, H., Fukui, K., Gamo, K., Tominari, Y., Takeuchi, H., Saga, Y., Hayashi, K., Kanemaki, M.T., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          Tips

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18343_B0Gfp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pUC-CAPbs_up_F2 (Pr0739)
          Region: insert 5'(GAL1-10 pro)
          Sequence file: RDB18343_B0Gfa.seq check
          >D07105B2_B0Gf_2_pUC-CAPbs_up_F2_B07_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGCTTTNNN CTTNATGCTT CCGGCTCGTA TGTTGTGTGG
             61 AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT
            121 CGAAATTAAC CCTCACTAAA GGGAACAAAA GCTGGTACCG GGCCCCCCCT CGAGGTCGAC
            181 GGTATCGATA AGCTTGATAT CGAATTCGAC AGGTTATCAG CAACAACACA GTCATATCCA
            241 TTCTCAATTA GCTCTACCAC AGTGTGTGAA CCAATGTATC CAGCACCACC TGTAACCAAA
            301 ACAATTTTAG AAGTACTTTC ACTTTGTAAC TGAGCTGTCA TTTATATTGA ATTTTCAAAA
            361 ATTCTTACTT TTTTTTTGGA TGGACGCAAA GAAGTTTAAT AATCATATTA CATGGCATTA
            421 CCACCATATA CATATCCATA TACATATCCA TATCTAATCT TACTTATATG TTGTGGAAAT
            481 GTAAAGAGCC CCATTATCTT AGCCTAAAAA AACCTTCTCT TTGGAACTTT CAGTAATACG
            541 CTTAACTGCT CATTGCTATA TTGAAGTACG GATTAGAAGC CGCCGAGCGG GTGACAGCCC
            601 TCCGAAGGAA GACTCTCCTC CGTGCGTCCT CGTCTTCACC GGTCGCGTTC CTGAAACGCA
            661 GATGTGCCTC GCGCCGCACT GCTCCGAACA ATAAAGATTC TACAATACTA GCTTTTATGG
            721 TTATGAAGAG GAAAAATTGG CAGTAACCTG NCCCCACAAA CCTTCAAATG AACGAATCAA
            781 ATTAACAACC ATAGGATGAT AATGCGATTA GTTTTTTAGC CTTATTTCTG GGGTAATTAA
            841 TCAGCGAANC GATGANTTTT GATCTATTAA CAGATATATA AATGCAAAAA CTGCATNACC
            901 ACTTTAACTA ANACTTTCNA CATTTTCNGN TNGTATTACT TCTTANNCNA ANGNAATAAA
            961 AAGTATCANC NAAAAANNTG NTNANATANC NNCNANACTT TNANNGTNNN NGNANNAAAA
          1021 ANNCNNNGNN NNNNNNNN
          //
          Primer: pBAC_lac_F (Pr0544)
          Region: insert mid(OsTIR1(F74A) 3')
          Sequence file: RDB18343_B0Gfb.seq check
          >D07105B2_B0Gf_2_pBAC_lac_F_C07_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN GNAANANGAC TNNCTATAGG GCGAANTGGA GCTCCNCCGC
             61 GGTGGCGGCC GCTCATAAAA TCTTGACGAA GTTAGGAGCA TCATCTCTGG CACCTGCGGT
            121 TGTTCTGTAG ACGTATAACT TTTCAACTTT AGGCAAGTCA CCATGGTTTT CTTCCATTTC
            181 ATTGGAACCA TCTCTTTCGT TAATAACTTC AACGTTCAAC ATTGGCATCT TTGAAGCTAA
            241 AACTTGACAA CCTTTCAAGG TAACGTTACA ACTGGACATC CACAAAGATC TCATTGTTTC
            301 ATATCTTGCG AAATTACCCA ACAAAGCTGC GTCACCAAAT GGAGAATCTC TGATTTCCAA
            361 CTTTCTTAAG TTCTTACAAC CGTTCATAAC ATGCATCATA CCCTTATCGG AGTCACCAGC
            421 GAATGCGATT GACAACATTT CCAATTGCTT AGCGTATTTA CCGATGTACA TAAAAACCTT
            481 ATCTGTCAAC AAACCACTGA TGGACAATCT TTGCAAACCC TTACACTCTC TAACGATTGC
            541 ACCAAAACCT TCATCTAAAG GTTGTGATGT AACAACATCA GGTTTACCTG GTTCCAAGAT
            601 ACACAATCTG AATCTAGTGA AGTTTGGACA ATTTTTAGCA ACAGTAACCA AAGCTGCGTT
            661 GGTCATTTGA TGACAGAAGT ACAACAAGGA GTTCAACTTA GGACAACCCA ATGAAACTGC
            721 AACTAAACCT TCTTCTGTAA CAGCAGAGTA ACCTGCAACA TAGAAATCAC TTGGAAAAAC
            781 TCTCAATTCT TGCAAATCCT TACAAGAAGA ANCAACAACT TGCAAACCTT TATCTGANAT
            841 ACAATCCAAA ACCCACAATC TTTGCAACTN NACACATCTA GAGNTCATCT TNNTCAAATC
            901 ACTAGCATCC AAAGTTGGTG CGNAAGANAA ANNNNAACNA NNCNANTGAN CACACNAAGG
            961 ATNTATAAAA GANAAACNAA CTGNNNNTNG CATCCCAAAA NNCNGNNNAA NNNNNNNNNN
          //
          Primer: GAL1pro_F2 (Pr0975)
          Region: insert mid(GAL1-10 pro,OsTIR1(F74A) 5')
          Sequence file: RDB18343_B0Gfc.seq check
          >D07105B2_B0Gf_2_GAL1pro_F2_C06_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNGNTNG NNNTACTTCT TATTCNNATG TAATAAAAGT ATCAACAAAA
             61 AATTGTTAAT ATACCTCTAT ACTTTAACGT CAAGGAGAAA AAACCCCGGA TCCACTAGTA
            121 TGACTTATTT TCCTGAAGAA GTTGTAGAAC ACATATTTTC CTTTTTACCT GCTCAAAGAG
            181 ATAGAAACAC CGTCTCCTTG GTCTGTAAGG TTTGGTACGA AATCGAAAGA TTGTCTAGAA
            241 GAGGTGTTTT TGTTGGTAAT TGTTACGCAG TTAGAGCTGG TAGAGTTGCT GCAAGATTCC
            301 CAAACGTTAG AGCATTGACT GTTAAAGGTA AACCTCATGC TGCTGATTTC AATTTGGTTC
            361 CACCTGATTG GGGTGGTTAT GCTGGTCCAT GGATTGAAGC TGCAGCTAGA GGTTGTCACG
            421 GTTTGGAAGA ATTGAGAATG AAGAGAATGG TTGTTTCTGA TGAATCATTG GAATTGTTAG
            481 CAAGATCTTT TCCTAGATTC AGAGCTTTGG TTTTGATCTC ATGTGAAGGT TTTTCCACCG
            541 ATGGTTTAGC AGCTGTTGCT TCACATTGTA AGTTGTTGAG AGAATTGGAT TTGCAAGAAA
            601 ACGAAGTTGA AGATAGAGGT CCAAGATGGT TATCTTGTTT TCCTGATTCC TGTACAAGTT
            661 TGGTTTCTTT GAACTTCGCA TGTATCAAGG GTGAAGTTAA CGCTGGTAGT TTGGAAAGAT
            721 TAGTTTCTAG ATCACCAAAT TTGAGATCTT TGAGATTGAA CAGATCCGTT AGTGTTGATA
            781 CCTTGGCANA GATCTTGTTG AGAACTCCTA ATTTGGAAGA NTTGGGTANT GGTAACTTGA
            841 CAGATGANTN NNNAACAGAA TCATACTTCA AGTTGANCNC CGCTTTGGAA AAGTGNAANA
            901 NGTTGAGATC NTTGNCANGN TTTNGGGANG NNNGNCCAGT TNGTTNNNCN TTTNNNTATC
          961 CNTTNNGNGN NNNNNTNNNN NNGNNTNNNN NTNNNNNNNN NN
          //
          Primer: URA3pro_F (Pr0170)
          Region: insert 3'(URA3 pro, URA3)
          Sequence file: RDB18343_B0Gfd.seq check
          >D07105B2_B0Gf_2_URA3pro_F_E07_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGANNTGCCN NGTATTCTTA ACCCAACTGC ACAGAACAAA
             61 AACCTGCAGG AAACGAAGAT AAATCATGTC GAAAGCTACA TATAAGGAAC GTGCTGCTAC
            121 TCATCCTAGT CCTGTTGCTG CCAAGCTATT TAATATCATG CACGAAAAGC AAACAAACTT
            181 GTGTGCTTCA TTGGATGTTC GTACCACCAA GGAATTACTG GAGTTAGTTG AAGCATTAGG
            241 TCCCAAAATT TGTTTACTAA AAACACATGT GGATATCTTG ACTGATTTTT CCATGGAGGG
            301 CACAGTTAAG CCGCTAAAGG CATTATCCGC CAAGTACAAT TTTTTACTCT TCGAAGACAG
            361 AAAATTTGCT GACATTGGTA ATACAGTCAA ATTGCAGTAC TCTGCGGGTG TATACAGAAT
            421 AGCAGAATGG GCAGACATTA CGAATGCACA CGGTGTGGTG GGCCCAGGTA TTGTTAGCGG
            481 TTTGAAGCAG GCGGCAGAAG AAGTAACAAA GGAACCTAGA GGCCTTTTGA TGTTAGCAGA
            541 ATTGTCATGC AAGGGCTCCC TATCTACTGG AGAATATACT AAGGGTACTG TTGACATTGC
            601 GAAGAGCGAC AAAGATTTTG TTATCGGCTT TATTGCTCAA AGAGACATGG GTGGAAGAGA
            661 TGAAGGTTAC GATTGGTTGA TTATGACACC CGGTGTGGGT TTAGATGACA AGGGAGACGC
            721 ATTGGGTCAA CAGTATAGAA CCGTGGATGA NGTGGTCTCT ACAGGATCTG ACATTATTAT
            781 TGTTGGAAGA GGANTATTTG CAAAGGGAAN GGANGCTAAN GTAGAGGGTG AANGTTACAG
            841 AAAAGCANGC TGGGAAGCAT ATTTGANAAG ANGCGGCCAG CAAAACTAAA AAAACTGTAT
            901 TATAAGTAAA TGNATGTATA CTAAACTCAC AAATTANAGC TTCNNNTTAA NTATATCAGT
          961 TATTANCCTA TGCNGNNGNN NANTACCGNN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]

          2024.03.26

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