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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK287 (mAID-Hygro)

A plasmid for construction of C-terminal mAID tagging donors.

Catalog number RDB13930
Resource name pMK287 (mAID-Hygro)
Alternative name mAID-Hygro, 1008
Clone info. A plasmid for construction of C-terminal mAID tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone.
Comment To make 0.5 M stock solution, dissolve indole-3-acetic acid (IAA) (Sigma-Aldrich, 45533) or 1-naphthaleneacetic acid (NAA) (Sigma-Aldrich, 35745) in 100 % DMSO. IAA solution can be made in 100 % ethanol. Aliquots can be kept for 6 months in the dark at -20oC (Devrekanli, A., Kanemaki, M.T. Methods in Molecular Biology, 1369, 257-278, 2016).
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA
Depositor|Developer Kanemaki, Masato |
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External Database
Arabidopsis thaliana AtIAA17

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB13930 pMK287 (mAID-Hygro) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13930_A6B8p1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Region: insert 5'(linker,mAID),SV40 pA,loxP, PGK pro
          Sequence file: RDB13930_A6B8d.seq check
          >D03070A14_A6B8_1_M13(-40)_G03_21.ab1
              1 NNNNNNNNNN NNNCNNANNN GANTNNCNAT AGGGCGAATT GGAGCTCCCC GGGNTCCGGT
             61 GCAGGCGCCA AGGAGAAGAG TGCTTGTCCT AAAGATCCAG CCAAACCTCC GGCCAAGGCA
            121 CAAGTTGTGG GATGGCCACC GGTGAGATCA TACCGGAAGA ACGTGATGGT TTCCTGCCAA
            181 AAATCAAGCG GTGGCCCGGA GGCGGCGGCG TTCGTGAAGG TATCAATGGA CGGAGCACCG
            241 TACTTGAGGA AAATCGATTT GAGGATGTAT AAATAATCTA GATAACTGAT CATAATCAGC
            301 CATACCACAT TTGTAGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC
            361 CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT
            421 TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT
            481 AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTAACGCG TCGATCATAT TCAATAACCC
            541 TTAATATAAC TTCGTATAAT GTATGCTATA CGAAGTTATT AGGTCTGAAG AGGAGTTTAC
            601 GTCCAGCCAA GCTTAGGATC TCGACCTCGA AATTCTACCG GGTAGGGGAG GCGCTTTTCC
            661 CAAGGCAGTC TGGAGCATGC GCTTTAGCAG CCCCGCTGGG CACTTGGCGC TACACAAGTG
            721 GCCTCTGGCC TCGCACACAT TCCACATCCA CCGGTAGGCG CCAACCGACT CCGTTCTTTG
            781 GTGGCCCCTT CGCGCCACCT TCTACTCCTC CCCTAGTCAG GAAGTTCCCC CCCGCCCCGC
            841 ANCTCGCGTC GTGCNNGNNG TGACAAATGG AANTANCACG TCTCACTANT CTCGNGCANA
            901 NGGACAGCAN CGCTNANCNA NGGAANCGGG NNNNNCTTNG GGGNANCNGN CNATANCANC
          961 TTNNNTNNNT NNCTTTCTGG GNNCNNNNNN NNNN
          //
          Primer: M13R
          Region: insert 5'(loxP,mouse PGK ter),HygR
          Sequence file: RDB13930_A6B8e.seq check
          >D03070A14_A6B8_1_M13R_C03_09.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNAAGGGN NNNNAAGCTG GGTACCGGAT CCTAGTGAAC
             61 CTCTTCGAGG GACCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ATTAAGGGTT
            121 CCGGATCTCG ACCAGCTTCT GATGGAATTA GAACTTGGCA AAACAATACT GAGAATGAAG
            181 TGTATGTGGA ACAGAGGCTG CTGATCTCGT TCTTCAGGCT ATGAAACTGA CACATTTGGA
            241 AACCACAGTA CTTAGAACCA CAAAGTGGGA ATCAAGAGAA AAACAATGAT CCCACGAGAG
            301 ATCTATAGAT CTATAGATCA TGAGTGGGAG GAATGAGCTG GCCCTTAATT TGGTTTTGCT
            361 TGTTTAAATT ATGATATCCA ACTATGAAAC ATTATCATAA AGCAATAGTA AAGAGCCTTC
            421 AGTAAAGAGC AGGCATTTAT CTAATCCCAC CCCACCCCCA CCCCCGTAGC TCCAATCCTT
            481 CCATTCAAAA TGTAGGTACT CTGTTCTCAC CCTTCTTAAC AAAGTATGAC AGGAAAAACT
            541 TCCATTTTAG TGGACATCTT TATTGTTTAA TAGATCATCA ATTTCTGCAT CCCGGGGATC
            601 TGATATCATC GATGCATGGG GTCGTGCGCT CCTTTCGGTC GGGCGCTGCG GGTCGTGGGG
            661 CGGGCGGCTA GCCTATTCCT TTGCCCTCGG ACGAGTGCTG GGGCGTCGGT TTCCACTATC
            721 GGCGAGTACT TCTACACAGC CATCGGTCCN GACGGCCGCG CTTCTGCGGG CGATTTGTGT
            781 ACGCCCGACA GTCCCGGCTC CGGATCGGAC GATTGCGTCN CATCGACCCT GCGCCCAANC
            841 TGCATCATCG AAATTGNCGT CNACCAANCT CTGATANAGT TGGTCNAGAC NAATGCGGAN
            901 CATATANNCC CNNGANCCGC NGNNATNCTG NNNGCTCCNG NANGCCTCCG CTCGAAGNNN
            961 CGCGNCTGNN GNTCCNNANN ANNCNANNNN NNGGNCNTNN NNNNNNNANG NTNNGNCNNN
          1021 CNNNNNNNNN GNNNNCCNNN NNNNNNN
          //
          Primer: PGKpro_F
          Region: HygR
          Sequence file: RDB13930_A6B8f.seq check
          >D03070A14_A6B8_1_PGK-proF_G07_19.ab1
              1 NNNNNNNNNN NNNNNNNNNN NTCTCCNGGC CTTTCGACCT GCATCCNTCT AGATCTCGAG
             61 CAGCTGAAGC TTACCGCTAG CATGGATAGA TCCGGAAAGC CTGAACTCAC CGCGACGTCT
            121 GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA GCTCTCGGAG
            181 GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT CCTGCGGGTA
            241 AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT TGCATCGGCC
            301 GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT GACCTATTGC
            361 ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA ACTGCCCGCT
            421 GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT TAGCCAGACG
            481 AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG GCGTGATTTC
            541 ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA CGACACCGTC
            601 AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA CTGCCCCGAA
            661 GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG TCCTGACGGA CAATGGCCGC
            721 ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG ATTCCCAATA CGAGGTCGCC
            781 AACATCTTCT TCTGGANGCC GTGGTTGGCT TGTATGGAGC AGCAGANGCG CTACTTCGAG
            841 CGGANGCATC CGGAGCTTGC ANGATCGCCG CGGCTNCNGG CGTATATGCT CCGCATTGGT
            901 CTTGANCNAC TCTATCANAN CTTGGTTGAN NNNNNTTTCN ATGANGCNNC TNNNNNCNNN
            961 NNNANGCNNC NNNNNNNTNC NNTNNNNANN NNNNNNGNNN GGNNNNNNNN NANNNNNNNN
          1021 NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.
          reference Chen, F.X., PAF1 regulation of promoter-proximal pause release via enhancer activation. Science 357 (6357): 1294-1298 (2017). PMID 28860207.
          reference Devrekanli, A., Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes. Methods in Molecular Biology 1369: 257-278 (2016). PMID 26519318.
          reference Nishimura, K., Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Curr. Protoc. Cell Biol. \64: 20.9.1-16 (2014). PMID 25181302.

          2022.07.07

          GNP_filter3_RDBDEP_html_220520.pl