Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK287 (mAID-Hygro)

A plasmid for construction of C-terminal mAID tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

Ordering

Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13930_A6B8p1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Region: insert 5'(linker,mAID),SV40 pA,loxP, PGK pro Sequence file: RDB13930_A6B8d.seq
>D03070A14_A6B8_1_M13(-40)_G03_21.ab1     1 NNNNNNNNNN NNNCNNANNN GANTNNCNAT AGGGCGAATT GGAGCTCCCC GGGNTCCGGT    61 GCAGGCGCCA AGGAGAAGAG TGCTTGTCCT AAAGATCCAG CCAAACCTCC GGCCAAGGCA   121 CAAGTTGTGG GATGGCCACC GGTGAGATCA TACCGGAAGA ACGTGATGGT TTCCTGCCAA   181 AAATCAAGCG GTGGCCCGGA GGCGGCGGCG TTCGTGAAGG TATCAATGGA CGGAGCACCG   241 TACTTGAGGA AAATCGATTT GAGGATGTAT AAATAATCTA GATAACTGAT CATAATCAGC   301 CATACCACAT TTGTAGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC   361 CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT   421 TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT   481 AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTAACGCG TCGATCATAT TCAATAACCC   541 TTAATATAAC TTCGTATAAT GTATGCTATA CGAAGTTATT AGGTCTGAAG AGGAGTTTAC   601 GTCCAGCCAA GCTTAGGATC TCGACCTCGA AATTCTACCG GGTAGGGGAG GCGCTTTTCC   661 CAAGGCAGTC TGGAGCATGC GCTTTAGCAG CCCCGCTGGG CACTTGGCGC TACACAAGTG   721 GCCTCTGGCC TCGCACACAT TCCACATCCA CCGGTAGGCG CCAACCGACT CCGTTCTTTG   781 GTGGCCCCTT CGCGCCACCT TCTACTCCTC CCCTAGTCAG GAAGTTCCCC CCCGCCCCGC   841 ANCTCGCGTC GTGCNNGNNG TGACAAATGG AANTANCACG TCTCACTANT CTCGNGCANA   901 NGGACAGCAN CGCTNANCNA NGGAANCGGG NNNNNCTTNG GGGNANCNGN CNATANCANC 961 TTNNNTNNNT NNCTTTCTGG GNNCNNNNNN NNNN //
Primer: M13R Region: insert 5'(loxP,mouse PGK ter),HygR Sequence file: RDB13930_A6B8e.seq
>D03070A14_A6B8_1_M13R_C03_09.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNAAGGGN NNNNAAGCTG GGTACCGGAT CCTAGTGAAC    61 CTCTTCGAGG GACCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ATTAAGGGTT   121 CCGGATCTCG ACCAGCTTCT GATGGAATTA GAACTTGGCA AAACAATACT GAGAATGAAG   181 TGTATGTGGA ACAGAGGCTG CTGATCTCGT TCTTCAGGCT ATGAAACTGA CACATTTGGA   241 AACCACAGTA CTTAGAACCA CAAAGTGGGA ATCAAGAGAA AAACAATGAT CCCACGAGAG   301 ATCTATAGAT CTATAGATCA TGAGTGGGAG GAATGAGCTG GCCCTTAATT TGGTTTTGCT   361 TGTTTAAATT ATGATATCCA ACTATGAAAC ATTATCATAA AGCAATAGTA AAGAGCCTTC   421 AGTAAAGAGC AGGCATTTAT CTAATCCCAC CCCACCCCCA CCCCCGTAGC TCCAATCCTT   481 CCATTCAAAA TGTAGGTACT CTGTTCTCAC CCTTCTTAAC AAAGTATGAC AGGAAAAACT   541 TCCATTTTAG TGGACATCTT TATTGTTTAA TAGATCATCA ATTTCTGCAT CCCGGGGATC   601 TGATATCATC GATGCATGGG GTCGTGCGCT CCTTTCGGTC GGGCGCTGCG GGTCGTGGGG   661 CGGGCGGCTA GCCTATTCCT TTGCCCTCGG ACGAGTGCTG GGGCGTCGGT TTCCACTATC   721 GGCGAGTACT TCTACACAGC CATCGGTCCN GACGGCCGCG CTTCTGCGGG CGATTTGTGT   781 ACGCCCGACA GTCCCGGCTC CGGATCGGAC GATTGCGTCN CATCGACCCT GCGCCCAANC   841 TGCATCATCG AAATTGNCGT CNACCAANCT CTGATANAGT TGGTCNAGAC NAATGCGGAN   901 CATATANNCC CNNGANCCGC NGNNATNCTG NNNGCTCCNG NANGCCTCCG CTCGAAGNNN   961 CGCGNCTGNN GNTCCNNANN ANNCNANNNN NNGGNCNTNN NNNNNNNANG NTNNGNCNNN 1021 CNNNNNNNNN GNNNNCCNNN NNNNNNN //
Primer: PGKpro_F Region: HygR Sequence file: RDB13930_A6B8f.seq
>D03070A14_A6B8_1_PGK-proF_G07_19.ab1     1 NNNNNNNNNN NNNNNNNNNN NTCTCCNGGC CTTTCGACCT GCATCCNTCT AGATCTCGAG    61 CAGCTGAAGC TTACCGCTAG CATGGATAGA TCCGGAAAGC CTGAACTCAC CGCGACGTCT   121 GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA GCTCTCGGAG   181 GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT CCTGCGGGTA   241 AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT TGCATCGGCC   301 GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT GACCTATTGC   361 ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA ACTGCCCGCT   421 GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT TAGCCAGACG   481 AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG GCGTGATTTC   541 ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA CGACACCGTC   601 AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA CTGCCCCGAA   661 GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG TCCTGACGGA CAATGGCCGC   721 ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG ATTCCCAATA CGAGGTCGCC   781 AACATCTTCT TCTGGANGCC GTGGTTGGCT TGTATGGAGC AGCAGANGCG CTACTTCGAG   841 CGGANGCATC CGGAGCTTGC ANGATCGCCG CGGCTNCNGG CGTATATGCT CCGCATTGGT   901 CTTGANCNAC TCTATCANAN CTTGGTTGAN NNNNNTTTCN ATGANGCNNC TNNNNNCNNN   961 NNNANGCNNC NNNNNNNTNC NNTNNNNANN NNNNNNGNNN GGNNNNNNNN NANNNNNNNN 1021 NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.07.07

GNP_filter3_RDBDEP_html_220520.pl