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pMK233

A donor vector for introducing CMV-AtAFB2 at the human AAVS1 locus.

Catalog number RDB18347
Resource name pMK233
Alternative name AAVS1 CMV-AtAFB2
Clone info. A donor vector for introducing CMV-AtAFB2 at the human AAVS1 locus, see Fig. 4A and SFig. 6 of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020. AtAFB2, a TIR1 paralog in Arabidopsis thaliana.
Vector backbone pCR (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell).
Gene/insert name Arabidopsis thaliana AFB2 cDNA human AAVS1 genomic
Depositor|Developer Kanemaki, Masato |
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External Database
Arabidopsis thaliana AFB2 human AAVS1

        Reference sequence
          
         
        Sequence (full) RDB18347zzk01.seq provided by the depositor

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
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        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
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        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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        RDB18347 pMK233 Under QC test. Please contact us. DNA solution

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        References and tips

        Original reference

        original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Auxin Inducible Degron (AID) System (English text)
        Featured content Auxin Inducible Degron (AID) System (Japanese text)

        QC test results

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


        References

        Original, user report and related articles

        original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522.

        2022.08.12

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