Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK233

A donor vector for introducing CMV-AtAFB2 at the human AAVS1 locus.

Sequence (full)	RDB18347zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18347_B0Gfp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-CAPbs_up_F2 (Pr0739) Region: lacO,T3 pro,AAVS1 arm1 5' Sequence file: RDB18347_B0Gfa.seq
>18347_18347_B0Gf_2_pUC-CAPbs_up_F2_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNCTTNN NNNNTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA    61 GCGGANAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG CTCAGAATTA   121 ACCCTCACTA AAGGGACTAG TCCTGCAGGT TTAAACGAAT TCGCCCTTTG CTTTCTCTGA   181 CCAGCATTCT CTCCCCTGGG CCTGTGCCGC TTTCTGTCTG CAGCTTGTGG CCTGGGTCAC   241 CTCTACGGCT GGCCCAGATC CTTCCCTGCC GCCTCCTTCA GGTTCCGTCT TCCTCCACTC   301 CCTCTTCCCC TTGCTCTCTG CTGTGTTGCT GCCCAAGGAT GCTCTTTCCG GAGCACTTCC   361 TTCTCGGCGC TGCACCACGT GATGTCCTCT GAGCGGATCC TCCCCGTGTC TGGGTCCTCT   421 CCGGGCATCT CTCCTCCCTC ACCCAACCCC ATGCCGTCTT CACTCGCTGG GTTCCCTTTT   481 CCTTCTCCTT CTGGGGCCTG TGCCATCTCT CGTTTCTTAN GATGGCCTTC TCCGACGGAT   541 GTCTCCCTTG CGTCCCGCCT CCCCTTCTTG TAGGCCTGCA TCATCACCGT TTTTCTGGAC   601 AACCCCAAAG TACCCCGTCT CCCTGGCTTT AGCCACCTCT CCATCCTCTT GCTTTCTTTG   661 CCTGGACACC CCGTTCTCCT GTGGATTCNG GTCACCTCTC ACTCCTTTCA TTTGGGCAGC   721 TCCCCTACCC CCCTTACCTC TCTAGTCTGT GCTAGCTCTT CNNGCCCCNT GTCATGGCAT   781 CTTCCNNGGG TCCNANAGCT CANCTAGTCT TCTTNCTNCN NNCNNGGNCC CTATGTNNNN   841 TTNNNNANAN NATGNTNNNN GNNTCNNNGG NTNNNGNGNC NCNNANCTNG NNNNNNNNTN 901 NNNNTNNCNN GNNNNNNNNN GNNNNNNNNG NNNNNNGNNN NNTTNNNNNN NNNN //
Primer: pUC-M13_-100 (Pr0310) Region: T7 pro,AAVS1 arm2 3' Sequence file: RDB18347_B0Gfb.seq
>18347_18347_B0Gf_2_pUC-M13_-100_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NGNTTTCNCN GTCACGACGT TGTAAAACGA CGGCCAGTGA    61 ATTGTAATAC GACTCACTAT AGGGCGAATT GAATTTAGCG GCCGCGAATT CGCCCTTAGA   121 GCAGAGCCAG GAACCCCTGT AGGGAAGGGG CAGGAGAGCC AGGGGCATGA GATGGTGGAC   181 GAGGAAGGGG GACAGGGAAG CCTGAGCGCC TCTCCTGGGC TTGCCAAGGA CTCAAACCCA   241 GAAGCCCAGA GCAGGGCCTT AGGGAAGCGG GACCCTGCTC TGGGCGGAGG AATATGTCCC   301 AGATAGCACT GGGGACTCTT TAAGGAAAGA AGGATGGAGA AAGAGAAAGG GAGTAGAGGC   361 GGCCACGACC TGGTGAACAC CTAGGACGCA CCATTCTCAC AAAGGGAGTT TTCCACACGG   421 ACACCCCCCT CCTCACCACA GCCCTGCCAG GACGGGGCTG GCTACTGGCC TTATCTCACA   481 GGTAAAACTG ACGCACGGAG GAACAATATA AATTGGGGAC TAGAAAGGTG AAGAGCCAAA   541 GTTAGAACTC AGGACCAACT TATTCTGATT TTGTTTTTCN NAACTGCTTC TCCTCTTGGG   601 AAGTGTAAGG AAGCTGCAGC ACCAGGATCA GTGAAACGCA CCAGACAGCC GCGTCAGANC   661 AGCTCANGTT CTGGGAGAGG GTAGCGCANG GTGGCCACTG AGAACCGGGC AGGTCACGCA   721 TCCCCCCCCT TCCCTCCCNN CCCCNGCNNA GCTCTCCCTC CNNGGATCCT CTCTGGNTCC   781 NTCGNAAGCA AANCNTANNN GNTCTGGNNN GGANAGAGNN GGNTCNNNGA AATGGGGGNG   841 NGTCNNNNGN NNNNNNATCT GCCNNANNNN NGNNGGGGNN NNNANNNNNN ATCNNNANNN 901 TANGNANNNN NNNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,AtAFB2 5' Sequence file: RDB18347_B0Gfc.seq
>18347_18347_B0Gf_2_CMV-Forward_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GNNGNNNNNG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT    61 ATAAGCAGAG CTGGTTTAGT GAACCGTCAG ATCCGCTAGC GCTGCCACCA TGAACTATTT   121 TCCTGACGAG GTGATTGAGC ACGTCTTTGA CTTCGTGACC AGCCACAAGG ATCGGAACGC   181 TATTAGCCTG GTGTGCAAGT CATGGTACAA GATCGAGAGA TACTCTAGGC AGAAAGTCTT   241 CATTGGCAAC TGTTATGCTA TCAACCCCGA GCGGTTGCTG CGGCGGTTCC CCTGCCTGAA   301 GTCTCTCACC TTGAAGGGGA AGCCCCACTT CGCAGACTTT AACCTGGTGC CACATGAGTG   361 GGGCGGGTTT GTGCTGCCAT GGATTGAGGC CTTGGCCCGG TCCAGGGTGG GGCTGGAGGA   421 GCTTAGGCTC AAGAGAATGG TGGTCACCGA TGAGTCCCTG GAGCTGCTCA GCAGGTCCTT   481 TGTCAACTTC AAGAGCCTCG TGCTCGTCAG CTGCGAGGGG TTCACCACTG ATGGGCTGGC   541 ATCTATCGCC GCTAACTGTA GACACCTGAG GGATCTCGAT CTGCAGGAAA ATGAAATCGA   601 CGATCACAGA GGGCAGTGGT TGTCATGTTT CCNCGACACT TGCACTACCC TGGTCACCCT   661 TAACTTTGCC TGCCTGGAGG GCGAAACCAA CCTGGTGGCC CTCGAACGGC TGGTGGCNNG   721 GAGCCNAAAC CTCAAATCTT TGAAGCTNNA NCGGGCCGTC CCCCTNNATG CTCTNGNTAG   781 GTNGATGGCC TGCGCCCCTC NNNNTGTGNA NCTGGGCNTN NNCTCCTANN ANAATGANCC   841 NGNNTNNNAN NNCTANCTCN NGCTGANNGN NNNNNATCNA NAANNGNNNN AGCTTNNNAN 901 NNNNGNNNNN NTTNCTNNNN NNCNNCCCNN NNNNNNNN //
Primer: SV40polyA_R2 (Pr0623) Region: AtAFB2 3' Sequence file: RDB18347_B0Gfd.seq
>18347_18347_B0Gf_2_SV40polyA_R2_F01_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNTTAACNNN NANAATTGCA TTCNTTTTAT GTTTCAGGTT    61 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT   121 GATTATGATC TAGAGTCGCG GCCCCGGTGG ATCCCGGGCC CGCGGTACCG TCGACGGGCC   181 CGTCGGATCC TCACAAAATC CACACAAATG GTGGGGCATC CATCCGTGTC CCGACGACGG   241 TTCTGTACAG GTACAGCTTA TCGACCTTCT GCCGTCCTTC ATGGCCGTTT TCCTCCATGC   301 GGTTGTTGTC GTTCTCGTTG ATAATCTCCA CGTTGAGCCA GGGGGCCTTC TCGGCGAGGC   361 GCTTGCAGCC GGACAGTGTC ACCTCGCAAC TACTCATCCA CAGACTGCGC ATAGTCTCGT   421 ACTTGCTCAC ATCAGCGAGA AGGGCTGTAT CTCCGAAAGG GCTATCCCGG ATCTCCAACT   481 TCTTCATTTT CTTGCAGCCG TTGAGCACGT AGAGCATCCC CTTATCTGTA TCGCCAGCAA   541 AAGCAATGCT CAGCATCTCC AGTTGGTTGG CGTACATCCC AATGTAAAGG AAGACCTGGT   601 CGGTCAGCAG TCCGGAGAGG CTAAGCCGCC TAANGGACTT GCAGGCCTTG ACGATTGCCC   661 CGAAGCCCTC GTCCAGGGGC TGANTTGTGA CGTGGTCAGG CTTGTTAGGC TCNAGGANGC   721 ACAACCTGAA CCTAATAAAG TTGGGGCAGT TCTTGGCCNC TGTCACAAGA NCGGCGTTAG   781 TCATCTGCTG ACNNAANTAC ANGATGGANT GCAACTTGGG GCATCCNGNN NNNANGGNCN   841 NCNNNCCNNC CTCTNNNNNN GCTGNGNNNC NNNCCNNNAN NANGNNNNAN GNNANNNNCN 901 NNANCTNNNN NNNNNN //
Primer: CMVd3_R (Pr0553) Region: CMV pro,CMV en,AAVS1 arm1 3' Sequence file: RDB18347_B0Gfe.seq
>18347_18347_B3BdF_2_CMVd3_R_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNTG NNNNNNNGGG CNNNNNNGTT ACGACNTTTT GGAAAGTCCC    61 GTTGATTTTG GTGCCAAAAC AAACTCCCAT TGACGTCAAT GGGGTGGAGA CTTGGAAATC   121 CCCGTGAGTC AAACCGCTAT CCACGCCCAT TGATGTACTG CCAAAACCGC ATCACCATGG   181 TAATAGCGAT GACTAATACG TAGATGTACT GCCAAGTAGG AAAGTCCCAT AAGGTCATGT   241 ACTGGGCATA ATGCCAGGCG GGCCATTTAC CGTCATTGAC GTCAATAGGG GGCGTACTTG   301 GCATATGATA CACTTGATGT ACTGCCAAGT GGGCAGTTTA CCGTAAATAC TCCACCCATT   361 GACGTCAATG GAAAGTCCCT ATTGGCGTTA CTATGGGAAC ATACGTCATT ATTGACGTCA   421 ATGGGCGGGG GTCGTTGGGC GGTCAGCCAG GCGGGCCATT TACCGTAAGT TATGTAACGC   481 GGAACTCCAT ATATGGGCTA TGAACTAATG ACCCCGTAAT TGATTACTAT TAATTGCCCC   541 ACTGTGGGGT GGAGGGGACA GATAAAAGTA CCCAGAACCA GAGCCACATT AACCGGCCCT   601 GGGAATATAA GGTGGTCCCA GCTCGGGGAC ACAGGATCCC TGGAGGCAGC AAACATGCTG   661 TCCTGAAGTG GACATAGGGG CCCGGGTTGG AGGAAGAAGA CTAGCTGAGC TCTCGGANCC   721 CTGGAANATG CNNTGACAGG GGGCTGGAAN ANCTAGCACA GACTANAGAG GTAANGGGGG   781 TANGGGAGCT GCCNNAATGA AAGGAGTGAN AGGTGANCCG AATCNNNNNG AGAACGGGGT   841 GTCCAGGCNA AGAAAGCNNN ANGANNNAGA GGTGGCTAAA GCCANGNNAN GGGNNACTTN   901 GGGGTTNTCC ANAAAAACNG TGATGATGCN NNCTACNANA NNGGGANGNN GGNCNNNNNG 961 GNNNNNNTNN NNNGNANANN NNNNNNNN //
Primer: hPGKpro_F2 (Pr0952) Region: hPGK pro,PuroR,bGH pA Sequence file: RDB18347_B0Gff.seq
>18347_18347_B3BdF_2_hPGKpro_F2_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNCG NNNGTCGGCA GTCGGCTCCC TCGTTGANCG    61 AATCACCGAC CTCTCTCCCC AGGGGGATCC ACCGGAGCTT ACCATGACCG AGTACAAGCC   121 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC   181 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT   241 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT   301 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC   361 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA   421 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC   481 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC   541 CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG   601 CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA   661 AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGAGGTACCC TGTGCCTTCT   721 AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC   781 ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GANTANGTGT   841 CATTCTATTC TGGGGGGGTG GGGTGGGGCA NGACAGCANG GGGGAGGANT GGGAAGANNA   901 TAGCANGCAT GCTGGGGNNG NNGNGGGCTC TATGGNNACT ANGGANNNGA TTGGTGACAN   961 AAAAGCCCCN TCCNTNNNNC NTNCNTNCTN NCNAGTCTNN NGANNNNNGG NTCTAANCCN 1021 NNNNNNNNN //
Primer: PuroR_F (Pr0554) Region: PuroR 3',bGH pA,AAVS1 arm2 5' Sequence file: RDB18347_B0Gfg.seq
>18347_18347_B3BdF_2_PuroR_F_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NGTGNCNGNA NGNNCGCGCA CCTGGTGCAT GACCCGCAAG    61 CCCGGTGCCT GAGGTACCCT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC   121 CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG   181 AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG   241 ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA   301 TGGTTACTAG GGACAGGATT GGTGACAGAA AAGCCCCATC CTTAGGCCTC CTCCTTCCTA   361 GTCTCCTGAT ATTGGGTCTA ACCCCCACCT CCTGTTAGGC AGATTCCTTA TCTGGTGACA   421 CACCCCCATT TCCTGGAGCC ATCTCTCTCC TTGCCAGAAC CTCTAAGGTT TGCTTACGAT   481 GGAGCCAGAG AGGATCCTGG GAGGGAGAGC TTGGCAGGGG GTGGGAGGGA AGGGGGGGAT   541 GCGTGACCTG CCCGGTTCTC AGTGGCCACC CTGCGCTACC CTCTCCCAGA ACCTGAGCTG   601 CTCTGACGCG GCTGTCTGGT GCGTTTCACT GATCCTGGTG CTGCAGCTTC CTTACACTTC   661 CCAAGAGGAG AAGCAGTTTG GAAAAACAAA ATCAGAATAA GTTGGTCCTG AGTTCTAACT   721 TTGGCTCTTC ACCTTTCTAG TCCCCAATTT ATATTGTTCC TCCGTGCGTC AGTTTTACCT   781 GTGAGATAAN GNCAGTAGCC AGCCCCGTCC TGNCANGGCT GTGGTGANGA GGGGGGTGTC   841 CGTGTGGAAA ACTCCCTTTG TGANAATGGT GCGTCCTNNG TGTTCACCAG GTCGTGGCCG   901 CCTCTACTCC CTTTCTCNTN CTNCNTCNNT CNTTCCNTNA AAGANTCCCN NNNTNCTATC   961 NNGGNNATAT TCCTNCNNCC CNGANNNNGN NNCCNCNTNN CCNTNNNNNC CNNNNNNNGG 1021 NCNTNNTGGN NTNNNNNNCN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl