Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK232 (AAVS1 CMV-OsTIR1-PURO)

A donor vector for introducing CMV-OsTIR1 at the human AAVS1 locus.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13918_A6B8p1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: insert mid (CMV pro, OsTIR1 5') Sequence file: RDB13918_A6B8f.seq
>D03070A02_A6B8_1_CMV_Forward_D10_10.ab1     1 ACGTCACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA    61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTG CCACCATGAC ATACTTTCCT   121 GAAGAGGTCG TCGAACACAT TTTTAGCTTC CTGCCTGCAC AGAGAGATAG AAACACAGTG   181 AGCCTGGTCT GCAAAGTGTG GTACGAGATC GAACGCCTGA GCCGGAGAGG AGTGTTCGTC   241 GGCAACTGCT ATGCTGTGAG AGCAGGCAGG GTCGCCGCTA GGTTTCCAAA TGTGCGCGCA   301 CTGACCGTCA AGGGGAAACC CCACTTCGCC GACTTTAACC TGGTGCCCCC TGATTGGGGA   361 GGATACGCCG GCCCTTGGAT CGAGGCAGCC GCTCGCGGCT GTCATGGACT GGAGGAACTG   421 CGCATGAAGC GAATGGTGGT CTCTGACGAA AGTCTGGAGC TGCTGGCTCG GAGCTTCCCT   481 AGGTTTCGCG CACTGGTGCT GATTTTCTTG CGAAAGGCTT CAGCACCGAT GGACTGGCAG   541 CCGTGGCCTC CCACTGTAAG CTGCTGCGGG GAGCTGGACC TTCCAGGAAG AATGGAGTGG   601 AGGATAGAGG GCCCAAATGG CTGTCTTGCT TCCCAGACTC ATGTACCAGC CTTGGTGTTC   661 CCTGAACTTT GCCTGCATCA AAGGCGAAAT GATGGCTGGG TCCCTGGGAC CGGCTGGGTT   721 TTCAAGAAGC CCACACTTGA AGGTCTCTTG CGGCTGGAAC CGGGAGCCTG GAGCGTGGAA   781 CACTCTGGGC TAAAATTCTG CTGGAGAACC CTTAACCTGG AGAATCTGGG AACGGCAATC   841 TCGACAGACG AATTTGGACA GGAGTCTACT ATTTTGCCTT CGGCTTTGGA AGTCAATGGC 901 GGGAGAGGGT CTCAGAAATC CGTGGAGTCT CACACCGGTT GTCC //
Primer: BGH_rev2 Region: insert mid (bGH p(A), PuroR,hPGK pro) Sequence file: RDB13918_A6B8g.seq
>D03070A02_A6B8_1_BGH_rev2_E10_13.ab1     1 CATCCGCGGT CAGGAGGCAC GGGGGAGGGG CAAACAACAG ATGGCTGGCA ACTAGAAGGC    61 ACAGGGTACC TCAGGCACCG GGCTTGCGGG TCATGCACCA GGTGCGCGGT CCTTCGGGCA   121 CCTCGACGTC GGCGGTGACG GTGAAGCCGA GCCGCTCGTA GAAGGGGAGG TTGCGGGGCG   181 CGGAGGTCTC CAGGAAGGCG GGCACCCCGG CGCGCTCGGC CGCCTCCACT CCGGGGAGCA   241 CGACGGCGCT GCCCAGACCC TTGCCCTGGT GGTCGGGCGA GACGCCGACG GTGGCCAGGA   301 ACCACGCGGG CTCCTTGGGC CGGTGCGGCG CCAGGAGGCC TTCCATCTGT TGCTGCGCGG   361 CCAGCCGGGA ACCGCTCAAC TCGGCCATGC GCGGGCCGAT CTCGGCGAAC ACCGCCCCCG   421 CTTCGACGCT CTCCGGCGTG GTCCAGACCG CCACCGCGGC GCCGTCGTCC GCGACCCACA   481 CCTTGCCGAT GTCGAGCCCG ACGCGCGTGA GGAAGAGTTC TTGCAGCTCG GTGACCCGCT   541 CGATGTGGCG GTCCGGATCG ACGGTGTGGC GCGTGGCGGG GTAGTCGGCG AACGCGGCGG   601 CGAGGGTGCG TACGGCCCTG GGGACGTCGT CGCGGGTGGC GAGGCGCACC GTGGGCTTGT   661 ACTCGGTCAT GGTAAGCTCC GGTGGATCCC CCTGGGGAGA GAGGTCGGTG ATTCGGTCAA   721 CGAGGGAGCC GACTGCCGAC GTGCGCTCCG GAGGCTTGCA GAATGCGGAA CACCGCGCGG   781 GCAGGAACAG GGCCCACACT ACCGCCCCAC ACCCCGCCTC CCGCACCGCC CCTTCCCGGC   841 CGCTGCTCTC GGCGCGCCCT GCTGAGCAGC CGCTATTGGC CACAGCCCAT CGCGGTCGGC   901 GCGCTGCCAT TGCTCCCTGG CGCTGTCCGT CTGCGAGGGT ACTAGTGAGA CGTGCGGCTT   961 CCGTTTGTCA CGTCCGGGCA CGCCGCGAAC CGCAAGGAAC CTTTCCCCGA CTTAGGGGCG  1021 GAGCAAGAAA GCGTCGCCTG GGGGTCCCAA CAAGGGTAGC CGGCTAAGAT CCGGATGACG  1081 CTGCCGAACC GAACGTGAAG AGTGTTGCCG AGACTCAGGA TCCGCGCCCG CTTGCCGTTT 1141 TCTCCCCGG //
Primer: M13R Region: insert 5' (AAVS1 arm1) Sequence file: RDB13918_A6B8h.seq
>D03070A02_A6B8_1_M13R_A10_01.ab1     1 CATCTGATAC CCTCACTAAA GGGACTAGTC CTGCAGGTTT AAACGAATTC GCCCTTTGCT    61 TTCTCTGACC AGCATTCTCT CCCCTGGGCC TGTGCCGCTT TCTGTCTGCA GCTTGTGGCC   121 TGGGTCACCT CTACGGCTGG CCCAGATCCT TCCCTGCCGC CTCCTTCAGG TTCCGTCTTC   181 CTCCACTCCC TCTTCCCCTT GCTCTCTGCT GTGTTGCTGC CCAAGGATGC TCTTTCCGGA   241 GCACTTCCTT CTCGGCGCTG CACCACGTGA TGTCCTCTGA GCGGATCCTC CCCGTGTCTG   301 GGTCCTCTCC GGGCATCTCT CCTCCCTCAC CCAACCCCAT GCCGTCTTCA CTCGCTGGGT   361 TCCCTTTTCC TTCTCCTTCT GGGGCCTGTG CCATCTCTCG TTTCTTAGGA TGGCCTTCTC   421 CGACGGATGT CTCCCTTGCG TCCCGCCTCC CCTTCTTGTA GGCCTGCATC ATCACCGTTT   481 TTCTGGACAA CCCCAAAGTA CCCCGTCTCC CTGGCTTTAG CCACCTCTCC ATCCTCTTGC   541 TTTCTTTGCC TGGACACCCC GTTCTCCTGT GGATTCGGGT CACCTCTCAC TCCTTTCATT   601 TGGGCAGCTC CCCTACCCCC CTTACTCTCT AGTCTGTGCT AGCTCTTCCA GCCCCCTGTC   661 ATGGCATCTT CCAAGGGTCC GAGAGCTCAG CTAGTCTTCT TCTCCACCGG GCCCCTATGT   721 CACTCAGACA GCATGTTTGC TGCCTCAAGG GATCTTGTGT CCCTAGCTGG ACCACCTTTA   781 ATTCCAGGGC GTAATGTGCT CTGATCTAGT ACTTATCTGT CCCTCCACCC CAATGGCATA   841 TAAATCATTC CGGCTAATAT GTCTAGCATA TGGAATCCGT TACATAACTT ACAGGTAATG //
Primer: M13_-40 Region: insert 3' (AAVS1 arm2) Sequence file: RDB13918_A6B8i.seq
>D03070A02_A6B8_1_M13(-40)_B10_04.ab1     1 CGCCGGCATG TATACGACTC ACTATAGGGC GAATTGAATT TAGCGGCCGC GAATTCGCCC    61 TTAGAGCAGA GCCAGGAACC CCTGTAGGGA AGGGGCAGGA GAGCCAGGGG CATGAGATGG   121 TGGACGAGGA AGGGGGACAG GGAAGCCTGA GCGCCTCTCC TGGGCTTGCC AAGGACTCAA   181 ACCCAGAAGC CCAGAGCAGG GCCTTAGGGA AGCGGGACCC TGCTCTGGGC GGAGGAATAT   241 GTCCCAGATA GCACTGGGGA CTCTTTAAGG AAAGAAGGAT GGAGAAAGAG AAAGGGAGTA   301 GAGGCGGCCA CGACCTGGTG AACACCTAGG ACGCACCATT CTCACAAAGG GAGTTTTCCA   361 CACGGACACC CCCCTCCTCA CCACAGCCCT GCCAGGACGG GGCTGGCTAC TGGCCTTATC   421 TCACAGGTAA AACTGACGCA CGGAGGAACA ATATAAATTG GGGACTAGAA AGGTGAAGAG   481 CCAAAGTTAG AACTCAGGAC CAACTTATTC TGATTTTGTT TTTCCAAACT GCTTCTCCTC   541 TTGGGAAGTG TAAGGAAGCT GCAGCACCAG GATCAGTGAA ACGCACCAGA CAGCCGCGTC   601 AGAGCAGCTC AGGTTCTGGG AGAGGGTAGC GCAGGGTGGC CACTGAGAAC CGGGCAGGTC   661 ACGCATCCCC CCCTTCCCTC CCACCCCCTG CCAAGCTCTC CCTCCCAGGA TCCTCTCTGG   721 CTCCATCGTA AGCAAACCTT AGAGGTTCTG GCAAGGAGAG AGATGGCTCC AGGAAATGGG   781 GGGTGTGTCA CCAGATAAGG AATCTGCCTA ACAGGAGGTG GGGGTTAGAC CCAATATCAG   841 AGACTAGGAA AGGAGGAGGC CTAAAGATGG GGCTTTTCTG TCACCAATCC TGTCCCTAGT   901 AACCAATAGA GCCCACCGCA TCCCCAGCAT GCCTGCTATT GTCTTCCCAA TCCTCCCCCT   961 TGCTGTCCTG CCCCCACCCC AACCCCCCAG ATAGATGACA CTACTCAGAA CAATGCGATG  1021 CAATTCCTTC ATTTTATAGA AGACAGTGGG GAGTGCACGT CATGGTCAAG GAAGCACGGG 1081 AGGGTCTACA ACGAATGCTG GCAACCTTAG GAAAAG //
Primer: SV40polyA-R Region: insert mid (OsTIR1 3') Sequence file: RDB13918_A6B8j.seq
>D03070A02_A6B8_1_SV40polyA-R_C10_07.ab1     1 GGTTTAGCAG TAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCTAGA GTCGCGGCCC    61 CGGTGGATCC CGGGCCCGCG GTACCGTCGA CGGGCCCGGG ATCCGATGGA TCCTCACAGA   121 ATCTTCACAA AGTTGGGAGC ATCATCTCTT GCCCCTGCGG TGGTCCGATA CACATACAGT   181 TTCTCCACCT TTGGCAGGTC GCCATGATTT TCCTCCATTT CGTTAGACCC GTCCCGCTCA   241 TTAATGACTT CCACGTTCAG CATAGGCATT TTACTAGCCA GGACCTGACA GCCCTTCAGA   301 GTCACATTGC AACTAGACAT CCACAGGCTC CTCATTGTCT CGTATCTGGC AAAGTTTCCC   361 AGCAGAGCGG CATCCCCGAA AGGGCTGTCC CGGATTTCCA GTTTTCGCAG ATTCTTACAC   421 CCGTTCATCA CGTGCATCAT GCCCTTATCG GAGTCTCCGG CAAATGCAAT GCTCAGCATC   481 TCCAGCTGCT TAGCATACTT GCCGATGTAC ATGAACACTT TGTCTGTCAG CAGGCCGGAA   541 ATGCTCAGCC TCTGGAGTCC CTTGCACTCT CTCACGATAG CTCCAAACCC TTCATCCAGT   601 GGCTGGGATG TGACCACGTC GGGTTTGCCT GGTTCCAGAA TACACAGCCG AAATCGGGTG   661 AAAATTAGGG CAGTTCTTGG CGACGGTCAC CAGTGCAGCA TTAGTCATCT GATGACAGAA 721 ATACAGCAGG CTGTTCAGCT TTGGGCATCC CAGTGAAGAC TGCCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2019.12.09

GNP_filter3_RDBDEP_html_191130.pl