Sequencing and PCR primers for primer information.
A donor vector for introducing CMV-OsTIR1 at the human AAVS1 locus.
| Catalog number | RDB13918 |
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| Resource name | pMK232 (AAVS1 CMV-OsTIR1-PURO) |
| Alternative name | AAVS1 CMV-OsTIR1-PURO |
| Clone info. | A donor vector for introducing CMV-OsTIR1 at the human AAVS1 locus. Please refer Fig. 3 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone. Oryza sativa OsTIR1 (NP_001052659.1), codon optimized cDNA for the expression in human cells. To make 0.5 M stock solution, dissolve indole-3-acetic acid (IAA) (Sigma-Aldrich, 45533) or 1-naphthaleneacetic acid (NAA) (Sigma-Aldrich, 35745) in 100 % DMSO. IAA solution can be made in 100 % ethanol. Aliquots can be kept for 6 months in the dark at -20oC (Devrekanli, A., Kanemaki, M.T. Methods in Molecular Biology, 1369, 257-278, 2016). |
| Vector backbone | pCR4Blunt-TOPO (Plasmid) |
| Size of vector backbone | 4.0 kb |
| Selectable markers | Am^r (E. coli), Puro^r (mammalian cells) |
| Gene/insert name | human AAVS1 genomic Oryza sativa OsTIR1 cDNA |
| Depositor | Kanemaki, Masato | |
| Other clones in our bank | human AAVS1 (NCBI Gene 17) | Oryza sativa | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB13918 | pMK232 (AAVS1 CMV-OsTIR1-PURO) | DNA solution |
Electronic file
Featured content
| Featured content | Auxin Inducible Degron (AID) System (English text) |
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| Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
References
| original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB13918_A6B8p1_v2.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: CMV-Forward | RDB13918_A6B8a_2.seq |
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>D03070A02_A6B8_1_CMV_Forward_D10_10.ab1 1 ACGTCACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTG CCACCATGAC ATACTTTCCT 121 GAAGAGGTCG TCGAACACAT TTTTAGCTTC CTGCCTGCAC AGAGAGATAG AAACACAGTG 181 AGCCTGGTCT GCAAAGTGTG GTACGAGATC GAACGCCTGA GCCGGAGAGG AGTGTTCGTC 241 GGCAACTGCT ATGCTGTGAG AGCAGGCAGG GTCGCCGCTA GGTTTCCAAA TGTGCGCGCA 301 CTGACCGTCA AGGGGAAACC CCACTTCGCC GACTTTAACC TGGTGCCCCC TGATTGGGGA 361 GGATACGCCG GCCCTTGGAT CGAGGCAGCC GCTCGCGGCT GTCATGGACT GGAGGAACTG 421 CGCATGAAGC GAATGGTGGT CTCTGACGAA AGTCTGGAGC TGCTGGCTCG GAGCTTCCCT 481 AGGTTTCGCG CACTGGTGCT GATTTTCTTG CGAAAGGCTT CAGCACCGAT GGACTGGCAG 541 CCGTGGCCTC CCACTGTAAG CTGCTGCGGG GAGCTGGACC TTCCAGGAAG AATGGAGTGG 601 AGGATAGAGG GCCCAAATGG CTGTCTTGCT TCCCAGACTC ATGTACCAGC CTTGGTGTTC 661 CCTGAACTTT GCCTGCATCA AAGGCGAAAT GATGGCTGGG TCCCTGGGAC CGGCTGGGTT 721 TTCAAGAAGC CCACACTTGA AGGTCTCTTG CGGCTGGAAC CGGGAGCCTG GAGCGTGGAA 781 CACTCTGGGC TAAAATTCTG CTGGAGAACC CTTAACCTGG AGAATCTGGG AACGGCAATC 841 TCGACAGACG AATTTGGACA GGAGTCTACT ATTTTGCCTT CGGCTTTGGA AGTCAATGGC 901 GGGAGAGGGT CTCAGAAATC CGTGGAGTCT CACACCGGTT GTCC // |
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| Primer: BGH_rev2 | RDB13918_A6B8b_2.seq |
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>D03070A02_A6B8_1_BGH_rev2_E10_13.ab1 1 CATCCGCGGT CAGGAGGCAC GGGGGAGGGG CAAACAACAG ATGGCTGGCA ACTAGAAGGC 61 ACAGGGTACC TCAGGCACCG GGCTTGCGGG TCATGCACCA GGTGCGCGGT CCTTCGGGCA 121 CCTCGACGTC GGCGGTGACG GTGAAGCCGA GCCGCTCGTA GAAGGGGAGG TTGCGGGGCG 181 CGGAGGTCTC CAGGAAGGCG GGCACCCCGG CGCGCTCGGC CGCCTCCACT CCGGGGAGCA 241 CGACGGCGCT GCCCAGACCC TTGCCCTGGT GGTCGGGCGA GACGCCGACG GTGGCCAGGA 301 ACCACGCGGG CTCCTTGGGC CGGTGCGGCG CCAGGAGGCC TTCCATCTGT TGCTGCGCGG 361 CCAGCCGGGA ACCGCTCAAC TCGGCCATGC GCGGGCCGAT CTCGGCGAAC ACCGCCCCCG 421 CTTCGACGCT CTCCGGCGTG GTCCAGACCG CCACCGCGGC GCCGTCGTCC GCGACCCACA 481 CCTTGCCGAT GTCGAGCCCG ACGCGCGTGA GGAAGAGTTC TTGCAGCTCG GTGACCCGCT 541 CGATGTGGCG GTCCGGATCG ACGGTGTGGC GCGTGGCGGG GTAGTCGGCG AACGCGGCGG 601 CGAGGGTGCG TACGGCCCTG GGGACGTCGT CGCGGGTGGC GAGGCGCACC GTGGGCTTGT 661 ACTCGGTCAT GGTAAGCTCC GGTGGATCCC CCTGGGGAGA GAGGTCGGTG ATTCGGTCAA 721 CGAGGGAGCC GACTGCCGAC GTGCGCTCCG GAGGCTTGCA GAATGCGGAA CACCGCGCGG 781 GCAGGAACAG GGCCCACACT ACCGCCCCAC ACCCCGCCTC CCGCACCGCC CCTTCCCGGC 841 CGCTGCTCTC GGCGCGCCCT GCTGAGCAGC CGCTATTGGC CACAGCCCAT CGCGGTCGGC 901 GCGCTGCCAT TGCTCCCTGG CGCTGTCCGT CTGCGAGGGT ACTAGTGAGA CGTGCGGCTT 961 CCGTTTGTCA CGTCCGGGCA CGCCGCGAAC CGCAAGGAAC CTTTCCCCGA CTTAGGGGCG 1021 GAGCAAGAAA GCGTCGCCTG GGGGTCCCAA CAAGGGTAGC CGGCTAAGAT CCGGATGACG 1081 CTGCCGAACC GAACGTGAAG AGTGTTGCCG AGACTCAGGA TCCGCGCCCG CTTGCCGTTT 1141 TCTCCCCGG // |
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| Primer: M13R | RDB13918_A6B8c_2.seq |
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>D03070A02_A6B8_1_M13R_A10_01.ab1 1 CATCTGATAC CCTCACTAAA GGGACTAGTC CTGCAGGTTT AAACGAATTC GCCCTTTGCT 61 TTCTCTGACC AGCATTCTCT CCCCTGGGCC TGTGCCGCTT TCTGTCTGCA GCTTGTGGCC 121 TGGGTCACCT CTACGGCTGG CCCAGATCCT TCCCTGCCGC CTCCTTCAGG TTCCGTCTTC 181 CTCCACTCCC TCTTCCCCTT GCTCTCTGCT GTGTTGCTGC CCAAGGATGC TCTTTCCGGA 241 GCACTTCCTT CTCGGCGCTG CACCACGTGA TGTCCTCTGA GCGGATCCTC CCCGTGTCTG 301 GGTCCTCTCC GGGCATCTCT CCTCCCTCAC CCAACCCCAT GCCGTCTTCA CTCGCTGGGT 361 TCCCTTTTCC TTCTCCTTCT GGGGCCTGTG CCATCTCTCG TTTCTTAGGA TGGCCTTCTC 421 CGACGGATGT CTCCCTTGCG TCCCGCCTCC CCTTCTTGTA GGCCTGCATC ATCACCGTTT 481 TTCTGGACAA CCCCAAAGTA CCCCGTCTCC CTGGCTTTAG CCACCTCTCC ATCCTCTTGC 541 TTTCTTTGCC TGGACACCCC GTTCTCCTGT GGATTCGGGT CACCTCTCAC TCCTTTCATT 601 TGGGCAGCTC CCCTACCCCC CTTACTCTCT AGTCTGTGCT AGCTCTTCCA GCCCCCTGTC 661 ATGGCATCTT CCAAGGGTCC GAGAGCTCAG CTAGTCTTCT TCTCCACCGG GCCCCTATGT 721 CACTCAGACA GCATGTTTGC TGCCTCAAGG GATCTTGTGT CCCTAGCTGG ACCACCTTTA 781 ATTCCAGGGC GTAATGTGCT CTGATCTAGT ACTTATCTGT CCCTCCACCC CAATGGCATA 841 TAAATCATTC CGGCTAATAT GTCTAGCATA TGGAATCCGT TACATAACTT ACAGGTAATG // |
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| Primer: M13_-40 | RDB13918_A6B8d_2.seq |
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>D03070A02_A6B8_1_M13(-40)_B10_04.ab1 1 CGCCGGCATG TATACGACTC ACTATAGGGC GAATTGAATT TAGCGGCCGC GAATTCGCCC 61 TTAGAGCAGA GCCAGGAACC CCTGTAGGGA AGGGGCAGGA GAGCCAGGGG CATGAGATGG 121 TGGACGAGGA AGGGGGACAG GGAAGCCTGA GCGCCTCTCC TGGGCTTGCC AAGGACTCAA 181 ACCCAGAAGC CCAGAGCAGG GCCTTAGGGA AGCGGGACCC TGCTCTGGGC GGAGGAATAT 241 GTCCCAGATA GCACTGGGGA CTCTTTAAGG AAAGAAGGAT GGAGAAAGAG AAAGGGAGTA 301 GAGGCGGCCA CGACCTGGTG AACACCTAGG ACGCACCATT CTCACAAAGG GAGTTTTCCA 361 CACGGACACC CCCCTCCTCA CCACAGCCCT GCCAGGACGG GGCTGGCTAC TGGCCTTATC 421 TCACAGGTAA AACTGACGCA CGGAGGAACA ATATAAATTG GGGACTAGAA AGGTGAAGAG 481 CCAAAGTTAG AACTCAGGAC CAACTTATTC TGATTTTGTT TTTCCAAACT GCTTCTCCTC 541 TTGGGAAGTG TAAGGAAGCT GCAGCACCAG GATCAGTGAA ACGCACCAGA CAGCCGCGTC 601 AGAGCAGCTC AGGTTCTGGG AGAGGGTAGC GCAGGGTGGC CACTGAGAAC CGGGCAGGTC 661 ACGCATCCCC CCCTTCCCTC CCACCCCCTG CCAAGCTCTC CCTCCCAGGA TCCTCTCTGG 721 CTCCATCGTA AGCAAACCTT AGAGGTTCTG GCAAGGAGAG AGATGGCTCC AGGAAATGGG 781 GGGTGTGTCA CCAGATAAGG AATCTGCCTA ACAGGAGGTG GGGGTTAGAC CCAATATCAG 841 AGACTAGGAA AGGAGGAGGC CTAAAGATGG GGCTTTTCTG TCACCAATCC TGTCCCTAGT 901 AACCAATAGA GCCCACCGCA TCCCCAGCAT GCCTGCTATT GTCTTCCCAA TCCTCCCCCT 961 TGCTGTCCTG CCCCCACCCC AACCCCCCAG ATAGATGACA CTACTCAGAA CAATGCGATG 1021 CAATTCCTTC ATTTTATAGA AGACAGTGGG GAGTGCACGT CATGGTCAAG GAAGCACGGG 1081 AGGGTCTACA ACGAATGCTG GCAACCTTAG GAAAAG // |
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| Primer: SV40polyA-R | RDB13918_A6B8e_2.seq |
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>D03070A02_A6B8_1_SV40polyA-R_C10_07.ab1 1 GGTTTAGCAG TAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCTAGA GTCGCGGCCC 61 CGGTGGATCC CGGGCCCGCG GTACCGTCGA CGGGCCCGGG ATCCGATGGA TCCTCACAGA 121 ATCTTCACAA AGTTGGGAGC ATCATCTCTT GCCCCTGCGG TGGTCCGATA CACATACAGT 181 TTCTCCACCT TTGGCAGGTC GCCATGATTT TCCTCCATTT CGTTAGACCC GTCCCGCTCA 241 TTAATGACTT CCACGTTCAG CATAGGCATT TTACTAGCCA GGACCTGACA GCCCTTCAGA 301 GTCACATTGC AACTAGACAT CCACAGGCTC CTCATTGTCT CGTATCTGGC AAAGTTTCCC 361 AGCAGAGCGG CATCCCCGAA AGGGCTGTCC CGGATTTCCA GTTTTCGCAG ATTCTTACAC 421 CCGTTCATCA CGTGCATCAT GCCCTTATCG GAGTCTCCGG CAAATGCAAT GCTCAGCATC 481 TCCAGCTGCT TAGCATACTT GCCGATGTAC ATGAACACTT TGTCTGTCAG CAGGCCGGAA 541 ATGCTCAGCC TCTGGAGTCC CTTGCACTCT CTCACGATAG CTCCAAACCC TTCATCCAGT 601 GGCTGGGATG TGACCACGTC GGGTTTGCCT GGTTCCAGAA TACACAGCCG AAATCGGGTG 661 AAAATTAGGG CAGTTCTTGG CGACGGTCAC CAGTGCAGCA TTAGTCATCT GATGACAGAA 721 ATACAGCAGG CTGTTCAGCT TTGGGCATCC CAGTGAAGAC TGCCA // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. |
|---|---|
| reference | Natsume, T., Acute inactivation of the replicative helicase in human cells triggers MCM8-9-dependent DNA synthesis. Genes Dev. (2017). PMID 28487407. |
| reference | Devrekanli, A., Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes. Methods in Molecular Biology 1369: 257-278 (2016). PMID 26519318. |
| reference | Nishimura, K., Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Curr. Protoc. Cell Biol. \64: 20.9.1-16 (2014). PMID 25181302 . |
2018.12.11
GNP_filter3_RDBDEP_html_181012.pl