Sequencing and PCR primers for primer information.
A plasmid for construction of N-terminal BSD-P2A-mAID-Nluc tagging donors.
| Catalog number | RDB16824 |
|---|---|
| Resource name | pMK407 (BSD-P2A-mAID-Nluc) |
| Clone info. | A plasmid for construction of N-terminal BSD-P2A-mAID-Nluc tagging donors. |
| Vector backbone | pBluescript (plasmid) |
| Size of vector backbone | 2.9 kb |
| Selectable markers | Am^r (E. coli) |
| Gene/insert name | Arabidopsis thaliana AtIAA17 cDNA Oplophorus gracilirostris luciferase cDNA |
| Depositor | Kanemaki, Masato | |
| Other clones in our bank | Arabidopsis thaliana AXR3 (KEGG orthology K14484) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
|---|---|
| Additional terms and conditions for distribution | The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation. |
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB16824 | pMK407 (BSD-P2A-mAID-Nluc) | DNA solution |
Electronic file
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Auxin Inducible Degron (AID) System (English text) |
|---|---|
| Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB16824_A8Lpp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pUC-M13_-100 | RDB16824_A8Lpa.seq |
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>D06157C2_A8Lp_1_pUC-M13_-100_G08_20_ABI24.ab1 1 GGGCGGTAAA GCGATTAAGT TGGGTACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC 61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGGTA CCGGGCCCCC 121 CCTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGCCACC ATGGCCAAGC CTTTGTCTCA 181 AGAAGAATCC ACCCTCATTG AAAGAGCAAC GGCTACAATC AACAGCATCC CCATCTCTGA 241 AGACTACAGC GTCGCCAGCG CAGCTCTCTC TAGCGACGGC CGCATCTTCA CTGGTGTCAA 301 TGTATATCAT TTTACTGGGG GACCTTGTGC AGAACTCGTG GTGCTGGGCA CTGCTGCTGC 361 TGCGGCAGCT GGCAACCTGA CTTGTATCGT CGCGATCGGA AATGAGAACA GGGGCATCTT 421 GAGCCCCTGC GGACGGTGCC GACAGGTGCT TCTCGATCTG CATCCTGGGA TCAAAGCCAT 481 AGTGAAGGAC AGTGATGGAC AGCCGACGGC AGTTGGGATT CGTGAATTGC TGCCCTCTGG 541 TTATGTGTGG GAGGGCTCAG GAAGCGGAGC TACTAACTTC AGCCTGCTGA AGCAGGCTGG 601 AGACGTGGAG GAGAACCCTG GACCTAGATC TAAGGAGAAG AGTGCTTGTC CTAAAGATCC 661 AGCCAAACCT CCGGCCAAGG CACAAGTTGT GGGATGGCCA CCGGTGAGAT CATACCGGAA 721 GAACGTGATG GTTTCCTGCC AAAAATCAAG CGGTGGCCCG GAGGCGGCGG CGTTCGTGAA 781 AGGTATCAAT GGACGGAGCA CCGTACTTGA GGAAAATCGA TTTGAGGATG TATAAAGCTA 841 GCATGGTCTT CACACTCGAA GATTTCGTTG GGGGACTGGC GACAGACAGC CGGCTACAAC 901 CCTGGACCAA GTCCTTGAAA CAGGGAGGTG TGTCCAGTTT GTTTTCAGAA TCTCGGGGTG 961 TCCGTAACTC CGATCCCAAA GATTGTCCCT GAGCGGTGAA AAATGGGCTG AAGATCGACA 1021 TCCAATGTCA TCATTCCTCG TATGACGTCT GAGCGGCCGA ACAATGGCCA GATCGAAATT 1081 TTAAGTTGGT GTTAACCCCT GGATG // |
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| Primer: reverse | RDB16824_A8Lpb.seq |
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>D06157C2_A8Lp_1_reverse_H08_23_ABI24.ab1 1 AAAGCCAATT AACCCTTCAC TAAAGGGAAC AAAAGCTGGA GCTCCACCGC GGTGGCGGCC 61 GCTCTAGAAC TAGTGGATCC GGCGCCGGCC CCTGCACCAG CGCCCGCCAG AATGCGTTCG 121 CACAGCCGCC AGCCGGTCAC TCCGTTGATG GTTACTCGGA ACAGCAGGGA GCCGTCGGGG 181 TTGATCAGGC GCTCGTCGAT AATTTTGTTG CCGTTCCACA GGGTCCCTGT TACAGTGATC 241 TTTTTGCCGT CGAACACGGC GATGCCTTCA TACGGCCGTC CGAAATAGTC GATCATGTTC 301 GGCGTAACCC CGTCGATTAC CAGTGTGCCA TAGTGCAGGA TCACCTTAAA GTGATGATCA 361 TCCACAGGGT ACACCACCTT AAAAATTTTT TCGATCTGGC CCATTTGGTC GCCGCTCAGA 421 CCTTCATACG GGATGATGAC ATGGATGTCG ATCTTCAGCC CATTTTCACC GCTCAGGACA 481 ATCCTTTGGA TCGGAGTTAC GGACACCCCG AGATTCTGAA ACAAACTGGA CACACCTCCC 541 TGTTCAAGGA CTTGGTCCAG GTTGTAGCCG GCTGTCTGTC GCCAGTCCCC AACGAAATCT 601 TCGAGTGTGA AGACCATGCT AGCTTTATAC ATCCTCAAAT CGATTTTCCT CAAGTACGGT 661 GCTCCGTCCA TTGATACCTT CACGAACGCC GCCGCCTCCG GGCCACCGCT TGATTTTTGG 721 CAGGAAACCA TCACGTTCTT CCGGTATGAT CTCACCGGTG GCCATCCCAC AACTTGTGCC 781 TTGGCCGGAG GTTTGGCTGG ATCTTTAGGA CAAGCACTCT TCTCCTTAGA TCTAGGTCCA 841 GGGTTCTCCT CCACGTCTCC AGCCTGCTTC AGCAGCTGAA GTTAGTAGCT CCGCTTCCCT 901 GAGCCCTCCC ACACATAAAC CAGAGGGCAG CAATTCACGA ATCCCCAACT GCCCGTCGCT 961 GTCCATCACT GTCCTTTCAC TATGGCCTTG GATCCCAAGG ATGCCAGATC GGAGAAGCAC 1021 CCTGGTCGTC CACGTTCCGG CAGGGCTTCA GGAATGGCCC CCCTTGGTTC TCAATTCCCG 1081 ATTCGCGAAC GATACCAAGT TCCAGGGATT TCGGC // |
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Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
2019.07.12
GNP_filter3_RDBDEP_html_190431.pl