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pMK407 (BSD-P2A-mAID-Nluc)

A plasmid for construction of N-terminal BSD-P2A-mAID-Nluc tagging donors.

Catalog number RDB16824
Resource name pMK407 (BSD-P2A-mAID-Nluc)
Clone info. A plasmid for construction of N-terminal BSD-P2A-mAID-Nluc tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Oplophorus gracilirostris luciferase cDNA
Depositor Kanemaki, Masato |
 
Other clones in our bank Arabidopsis thaliana AXR3 (KEGG orthology K14484) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16824 pMK407 (BSD-P2A-mAID-Nluc) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16824_A8Lpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 RDB16824_A8Lpa.seq
>D06157C2_A8Lp_1_pUC-M13_-100_G08_20_ABI24.ab1
    1 GGGCGGTAAA GCGATTAAGT TGGGTACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC
   61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGGTA CCGGGCCCCC
  121 CCTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGCCACC ATGGCCAAGC CTTTGTCTCA
  181 AGAAGAATCC ACCCTCATTG AAAGAGCAAC GGCTACAATC AACAGCATCC CCATCTCTGA
  241 AGACTACAGC GTCGCCAGCG CAGCTCTCTC TAGCGACGGC CGCATCTTCA CTGGTGTCAA
  301 TGTATATCAT TTTACTGGGG GACCTTGTGC AGAACTCGTG GTGCTGGGCA CTGCTGCTGC
  361 TGCGGCAGCT GGCAACCTGA CTTGTATCGT CGCGATCGGA AATGAGAACA GGGGCATCTT
  421 GAGCCCCTGC GGACGGTGCC GACAGGTGCT TCTCGATCTG CATCCTGGGA TCAAAGCCAT
  481 AGTGAAGGAC AGTGATGGAC AGCCGACGGC AGTTGGGATT CGTGAATTGC TGCCCTCTGG
  541 TTATGTGTGG GAGGGCTCAG GAAGCGGAGC TACTAACTTC AGCCTGCTGA AGCAGGCTGG
  601 AGACGTGGAG GAGAACCCTG GACCTAGATC TAAGGAGAAG AGTGCTTGTC CTAAAGATCC
  661 AGCCAAACCT CCGGCCAAGG CACAAGTTGT GGGATGGCCA CCGGTGAGAT CATACCGGAA
  721 GAACGTGATG GTTTCCTGCC AAAAATCAAG CGGTGGCCCG GAGGCGGCGG CGTTCGTGAA
  781 AGGTATCAAT GGACGGAGCA CCGTACTTGA GGAAAATCGA TTTGAGGATG TATAAAGCTA
  841 GCATGGTCTT CACACTCGAA GATTTCGTTG GGGGACTGGC GACAGACAGC CGGCTACAAC
  901 CCTGGACCAA GTCCTTGAAA CAGGGAGGTG TGTCCAGTTT GTTTTCAGAA TCTCGGGGTG
  961 TCCGTAACTC CGATCCCAAA GATTGTCCCT GAGCGGTGAA AAATGGGCTG AAGATCGACA
 1021 TCCAATGTCA TCATTCCTCG TATGACGTCT GAGCGGCCGA ACAATGGCCA GATCGAAATT
1081 TTAAGTTGGT GTTAACCCCT GGATG
//
Primer: reverse RDB16824_A8Lpb.seq
>D06157C2_A8Lp_1_reverse_H08_23_ABI24.ab1
    1 AAAGCCAATT AACCCTTCAC TAAAGGGAAC AAAAGCTGGA GCTCCACCGC GGTGGCGGCC
   61 GCTCTAGAAC TAGTGGATCC GGCGCCGGCC CCTGCACCAG CGCCCGCCAG AATGCGTTCG
  121 CACAGCCGCC AGCCGGTCAC TCCGTTGATG GTTACTCGGA ACAGCAGGGA GCCGTCGGGG
  181 TTGATCAGGC GCTCGTCGAT AATTTTGTTG CCGTTCCACA GGGTCCCTGT TACAGTGATC
  241 TTTTTGCCGT CGAACACGGC GATGCCTTCA TACGGCCGTC CGAAATAGTC GATCATGTTC
  301 GGCGTAACCC CGTCGATTAC CAGTGTGCCA TAGTGCAGGA TCACCTTAAA GTGATGATCA
  361 TCCACAGGGT ACACCACCTT AAAAATTTTT TCGATCTGGC CCATTTGGTC GCCGCTCAGA
  421 CCTTCATACG GGATGATGAC ATGGATGTCG ATCTTCAGCC CATTTTCACC GCTCAGGACA
  481 ATCCTTTGGA TCGGAGTTAC GGACACCCCG AGATTCTGAA ACAAACTGGA CACACCTCCC
  541 TGTTCAAGGA CTTGGTCCAG GTTGTAGCCG GCTGTCTGTC GCCAGTCCCC AACGAAATCT
  601 TCGAGTGTGA AGACCATGCT AGCTTTATAC ATCCTCAAAT CGATTTTCCT CAAGTACGGT
  661 GCTCCGTCCA TTGATACCTT CACGAACGCC GCCGCCTCCG GGCCACCGCT TGATTTTTGG
  721 CAGGAAACCA TCACGTTCTT CCGGTATGAT CTCACCGGTG GCCATCCCAC AACTTGTGCC
  781 TTGGCCGGAG GTTTGGCTGG ATCTTTAGGA CAAGCACTCT TCTCCTTAGA TCTAGGTCCA
  841 GGGTTCTCCT CCACGTCTCC AGCCTGCTTC AGCAGCTGAA GTTAGTAGCT CCGCTTCCCT
  901 GAGCCCTCCC ACACATAAAC CAGAGGGCAG CAATTCACGA ATCCCCAACT GCCCGTCGCT
  961 GTCCATCACT GTCCTTTCAC TATGGCCTTG GATCCCAAGG ATGCCAGATC GGAGAAGCAC
 1021 CCTGGTCGTC CACGTTCCGG CAGGGCTTCA GGAATGGCCC CCCTTGGTTC TCAATTCCCG
1081 ATTCGCGAAC GATACCAAGT TCCAGGGATT TCGGC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

2019.07.12

GNP_filter3_RDBDEP_html_190431.pl