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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK407 (BSD-P2A-mAID-Nluc)

A plasmid for construction of N-terminal BSD-P2A-mAID-Nluc tagging donors.

Catalog number RDB16824
Resource name pMK407 (BSD-P2A-mAID-Nluc)
Clone info. A plasmid for construction of N-terminal BSD-P2A-mAID-Nluc tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Oplophorus gracilirostris luciferase cDNA
Depositor|Developer Kanemaki, Masato |
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External Database
Arabidopsis thaliana AtIAA17 Oplophorus gracilirostris luciferase

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16824 pMK407 (BSD-P2A-mAID-Nluc) DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16824_A8Lpp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pUC-M13_-100
          Sequence file: RDB16824_A8Lpa.seq check
          >D06157C2_A8Lp_1_pUC-M13_-100_G08_20_ABI24.ab1
              1 GGGCGGTAAA GCGATTAAGT TGGGTACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC
             61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGGTA CCGGGCCCCC
            121 CCTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGCCACC ATGGCCAAGC CTTTGTCTCA
            181 AGAAGAATCC ACCCTCATTG AAAGAGCAAC GGCTACAATC AACAGCATCC CCATCTCTGA
            241 AGACTACAGC GTCGCCAGCG CAGCTCTCTC TAGCGACGGC CGCATCTTCA CTGGTGTCAA
            301 TGTATATCAT TTTACTGGGG GACCTTGTGC AGAACTCGTG GTGCTGGGCA CTGCTGCTGC
            361 TGCGGCAGCT GGCAACCTGA CTTGTATCGT CGCGATCGGA AATGAGAACA GGGGCATCTT
            421 GAGCCCCTGC GGACGGTGCC GACAGGTGCT TCTCGATCTG CATCCTGGGA TCAAAGCCAT
            481 AGTGAAGGAC AGTGATGGAC AGCCGACGGC AGTTGGGATT CGTGAATTGC TGCCCTCTGG
            541 TTATGTGTGG GAGGGCTCAG GAAGCGGAGC TACTAACTTC AGCCTGCTGA AGCAGGCTGG
            601 AGACGTGGAG GAGAACCCTG GACCTAGATC TAAGGAGAAG AGTGCTTGTC CTAAAGATCC
            661 AGCCAAACCT CCGGCCAAGG CACAAGTTGT GGGATGGCCA CCGGTGAGAT CATACCGGAA
            721 GAACGTGATG GTTTCCTGCC AAAAATCAAG CGGTGGCCCG GAGGCGGCGG CGTTCGTGAA
            781 AGGTATCAAT GGACGGAGCA CCGTACTTGA GGAAAATCGA TTTGAGGATG TATAAAGCTA
            841 GCATGGTCTT CACACTCGAA GATTTCGTTG GGGGACTGGC GACAGACAGC CGGCTACAAC
            901 CCTGGACCAA GTCCTTGAAA CAGGGAGGTG TGTCCAGTTT GTTTTCAGAA TCTCGGGGTG
            961 TCCGTAACTC CGATCCCAAA GATTGTCCCT GAGCGGTGAA AAATGGGCTG AAGATCGACA
           1021 TCCAATGTCA TCATTCCTCG TATGACGTCT GAGCGGCCGA ACAATGGCCA GATCGAAATT
          1081 TTAAGTTGGT GTTAACCCCT GGATG
          //
          Primer: reverse
          Sequence file: RDB16824_A8Lpb.seq check
          >D06157C2_A8Lp_1_reverse_H08_23_ABI24.ab1
              1 AAAGCCAATT AACCCTTCAC TAAAGGGAAC AAAAGCTGGA GCTCCACCGC GGTGGCGGCC
             61 GCTCTAGAAC TAGTGGATCC GGCGCCGGCC CCTGCACCAG CGCCCGCCAG AATGCGTTCG
            121 CACAGCCGCC AGCCGGTCAC TCCGTTGATG GTTACTCGGA ACAGCAGGGA GCCGTCGGGG
            181 TTGATCAGGC GCTCGTCGAT AATTTTGTTG CCGTTCCACA GGGTCCCTGT TACAGTGATC
            241 TTTTTGCCGT CGAACACGGC GATGCCTTCA TACGGCCGTC CGAAATAGTC GATCATGTTC
            301 GGCGTAACCC CGTCGATTAC CAGTGTGCCA TAGTGCAGGA TCACCTTAAA GTGATGATCA
            361 TCCACAGGGT ACACCACCTT AAAAATTTTT TCGATCTGGC CCATTTGGTC GCCGCTCAGA
            421 CCTTCATACG GGATGATGAC ATGGATGTCG ATCTTCAGCC CATTTTCACC GCTCAGGACA
            481 ATCCTTTGGA TCGGAGTTAC GGACACCCCG AGATTCTGAA ACAAACTGGA CACACCTCCC
            541 TGTTCAAGGA CTTGGTCCAG GTTGTAGCCG GCTGTCTGTC GCCAGTCCCC AACGAAATCT
            601 TCGAGTGTGA AGACCATGCT AGCTTTATAC ATCCTCAAAT CGATTTTCCT CAAGTACGGT
            661 GCTCCGTCCA TTGATACCTT CACGAACGCC GCCGCCTCCG GGCCACCGCT TGATTTTTGG
            721 CAGGAAACCA TCACGTTCTT CCGGTATGAT CTCACCGGTG GCCATCCCAC AACTTGTGCC
            781 TTGGCCGGAG GTTTGGCTGG ATCTTTAGGA CAAGCACTCT TCTCCTTAGA TCTAGGTCCA
            841 GGGTTCTCCT CCACGTCTCC AGCCTGCTTC AGCAGCTGAA GTTAGTAGCT CCGCTTCCCT
            901 GAGCCCTCCC ACACATAAAC CAGAGGGCAG CAATTCACGA ATCCCCAACT GCCCGTCGCT
            961 GTCCATCACT GTCCTTTCAC TATGGCCTTG GATCCCAAGG ATGCCAGATC GGAGAAGCAC
           1021 CCTGGTCGTC CACGTTCCGG CAGGGCTTCA GGAATGGCCC CCCTTGGTTC TCAATTCCCG
          1081 ATTCGCGAAC GATACCAAGT TCCAGGGATT TCGGC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

          2021.11.02

          GNP_filter3_RDBDEP_html_211029.pl