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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK284 (Stag-FLAG-Hygro)

A plasmid for construction of C-terminal Stag-3FLAG tagging donors.

Catalog number RDB13927
Resource name pMK284 (Stag-FLAG-Hygro)
Alternative name Stag-FLAG-Hygro
Clone info. A plasmid for construction of C-terminal Stag-3FLAG tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone.
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Depositor|Developer Kanemaki, Masato |
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            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB13927 pMK284 (Stag-FLAG-Hygro) DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Auxin Inducible Degron (AID) System (English text)
            Featured content Auxin Inducible Degron (AID) System (Japanese text)
            Featured content Genome Editing (English text)
            Featured content Genome Editing (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13927_A6B8p1_v2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: M13_-40
            Sequence file: RDB13927_A6B8a_2.seq check
            >D03070A11_A6B8_4_M13(-40)_E02_14.ab1
                1 CGCCATGCGC GCGCGTATAC GACTCACTAT AGGGCGAATT GGAGCTCGGA TCCGGTGCAG
               61 GCGCCAAGGA AACCGCCGCT GCAAAATTCG AACGCCAGCA CATGGACAGC CGATCCCGGG
              121 CTGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG
              181 ATGACAAGTA GTGTACAAGT AATCTAGATA ACTGATCATA ATCAGCCATA CCACATTTGT
              241 AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA AACATAAAAT
              301 GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA
              361 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC
              421 CAAACTCATC AATGTATCTT AACGCGTCGA TCATATTCAA TAACCCTTAA TATAACTTCG
              481 TATAATGTAT GCTATACGAA GTTATTAGGT CTGAAGAGGA GTTTACGTCC AGCCAAGCTT
              541 AGGATCTCGA CCTCGAAATT CTACCGGGTA GGGGAGGCGC TTTTCCCAAG GCAGTCTGGA
              601 GCATGCGCTT TAGCAGCCCC GCTGGGCACT TGGCGCTACA CAAGTGGCCT CTGGCCTCGC
              661 ACACATTCCA CATCCACCGG TAGGCGCCAA CCGACTCCGT TCTTTGGTGG CCCCTTCGCG
              721 CCACCTTCTA CTCCTCCCCT AGTCAGGAAG TTCCCCCCCG CCCCGCAGCT CGCGTCGTGC
              781 AGGACGTGAC AAATGGAAGT AGCACGTCTC ACTAGTCTCG TGCAGATGGA CAGCACCGCT
              841 GAGCAATGGA AGCGGGTAGG CCTTTGGGGC AGCGGCCAAT AGCAGCTTTG CTCCTTCGCT
              901 TTCTGGGCTC AGAGGCTGGG AAGGGGTGGG TCCCGGGGCG GGCTCACGGG CGGGCTCAGG
              961 GCGGGGCGGG CGCCCGAAAG TCCTCCCGGA GGCCCGGCAT TCTGCACGCT TCAAAGCGCA
             1021 CGTCTGCCGG CGCTGTTCTC CTCTTCCTCA TCTCCAGGGC CCTTTCGACC CTGCCATCAA
             1081 TCCTAGATCT CGGAGGCAGC TGAAGGCTAA CGCTTAGGCA TGCATAGATC GGAAGTCGTT
            1141 GACTTCACGC GACGTCTGTC CGAGAAAAGG ACTACCATGG TA
            //
            Primer: PGKpro_F
            Sequence file: RDB13927_A6B8b_2.seq check
            >D03070A11_A6B8_4_PGK-proF_F02_17.ab1
                1 AGCGGGCGTA TCTCTCTTCT CATCTCCGGG CCTTTCGACC TGCATCCATC TAGATCTCGA
               61 GCAGCTGAAG CTTACCGCTA GCATGGATAG ATCCGGAAAG CCTGAACTCA CCGCGACGTC
              121 TGTCGAGAAG TTTCTGATCG AAAAGTTCGA CAGCGTCTCC GACCTGATGC AGCTCTCGGA
              181 GGGCGAAGAA TCTCGTGCTT TCAGCTTCGA TGTAGGAGGG CGTGGATATG TCCTGCGGGT
              241 AAATAGCTGC GCCGATGGTT TCTACAAAGA TCGTTATGTT TATCGGCACT TTGCATCGGC
              301 CGCGCTCCCG ATTCCGGAAG TGCTTGACAT TGGGGAATTC AGCGAGAGCC TGACCTATTG
              361 CATCTCCCGC CGTGCACAGG GTGTCACGTT GCAAGACCTG CCTGAAACCG AACTGCCCGC
              421 TGTTCTGCAG CCGGTCGCGG AGGCCATGGA TGCGATCGCT GCGGCCGATC TTAGCCAGAC
              481 GAGCGGGTTC GGCCCATTCG GACCGCAAGG AATCGGTCAA TACACTACAT GGCGTGATTT
              541 CATATGCGCG ATTGCTGATC CCCATGTGTA TCACTGGCAA ACTGTGATGG ACGACACCGT
              601 CAGTGCGTCC GTCGCGCAGG CTCTCGATGA GCTGATGCTT TGGGCCGAGG ACTGCCCCGA
              661 AGTCCGGCAC CTCGTGCACG CGGATTTCGG CTCCAACAAT GTCCTGACGG ACAATGGCCG
              721 CATAACAGCG GTCATTGACT GGAGCGAGGC GATGTTCGGG GATTCCCAAT ACGAGGTCGC
              781 CAACATCTTC TTCTGGAGGC CGTGGTTGGC TTGTATGGAG CAGCAGACGC GCTACTTCGA
              841 GCGGAGGCAT CCGGAGCTTG CAGGATCGCC GCGGCTCCGG GCGTATATGC TCCGCATTGG
              901 TCTTGACCAA CTCTATCAGA GCTTGGTTGA CGGCAATTTC GATGATGCAG CTTGGGCGCA
              961 GGGTCGATGC GACGCAATCG TCCCGATCCC GGAGCCCGGG ACTGTCGGCC GTACACAAAT
             1021 CGCCCCGCAG AAGCGGCGGG CCCGTCCTGG ACCGATGGCC TGTGTAGAAG GTACTCGGCC
             1081 GATAGTGGAA CCCGGAACGC CCAGCACTTC GTTCGAAGGG CAAAGGAAAT ACGGCTTAAG
            1141 GCCCGTCGGC
            //
            Primer: M13R
            Sequence file: RDB13927_A6B8c_2.seq check
            >D03070A11_A6B8_4_M13R_D02_11.ab1
                1 CTCGCGCATT ACCTCACTAA AGGGACAAAA GCTGGGTACC GGATCCTAGT GAACCTCTTC
               61 GAGGGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG GGTTCCGGAT
              121 CTCGACCAGC TTCTGATGGA ATTAGAACTT GGCAAAACAA TACTGAGAAT GAAGTGTATG
              181 TGGAACAGAG GCTGCTGATC TCGTTCTTCA GGCTATGAAA CTGACACATT TGGAAACCAC
              241 AGTACTTAGA ACCACAAAGT GGGAATCAAG AGAAAAACAA TGATCCCACG AGAGATCTAT
              301 AGATCTATAG ATCATGAGTG GGAGGAATGA GCTGGCCCTT AATTTGGTTT TGCTTGTTTA
              361 AATTATGATA TCCAACTATG AAACATTATC ATAAAGCAAT AGTAAAGAGC CTTCAGTAAA
              421 GAGCAGGCAT TTATCTAATC CCACCCCACC CCCACCCCCG TAGCTCCAAT CCTTCCATTC
              481 AAAATGTAGG TACTCTGTTC TCACCCTTCT TAACAAAGTA TGACAGGAAA AACTTCCATT
              541 TTAGTGGACA TCTTTATTGT TTAATAGATC ATCAATTTCT GCATCCCGGG GATCTGATAT
              601 CATCGATGCA TGGGGTCGTG CGCTCCTTTC GGTCGGGCGC TGCGGGTCGT GGGGCGGGCG
              661 GCTAGCCTAT TCCTTTGCCC TCGGACGAGT GCTGGGGCGT CGGTTTCCAC TATCGGCGAG
              721 TACTTCTACA CAGCCATCGG TCCAGACGGC CGCGCTTCTG CGGGCGATTT GTGTACGCCC
              781 GACAGTCCCG GCTCCGGATC GGACGATTGC GTCGCATCGA CACTGCGCCC AAGCTGCATC
              841 ATCGAAATTG CCGTCAACCA AGCTCTGATA GAGTTGGTCA AGACCAATGC GGAGCATATA
              901 CGCCCGGAGC CGCGGCGATC CTGCAAGCTC CGGATGCCTC CGCTCGAAGT AGCGCGTCTG
              961 CTGCTCCATA CAAGCCAACC CACGGCCCTC CAGAGAGATG TTTGGGCGAC CCTCGTAATT
             1021 GGGAAATCCC CCGACATCGC CCTCGCCTCA GTTCATTGAA CCGGCTTGCT ATGCGGTCCA
            1081 TGATCAGTCA GGCACCATTG ATTTGGAAGC CGGAAAATTC CCGGACG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl