Sequencing and PCR primers for primer information.
A plasmid for construction of C-terminal Stag-3FLAG tagging donors.
| Catalog number | RDB13927 |
|---|---|
| Resource name | pMK284 (Stag-FLAG-Hygro) |
| Alternative name | Stag-FLAG-Hygro |
| Clone info. | A plasmid for construction of C-terminal Stag-3FLAG tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone. |
| Vector backbone | pBluescriptII KS- (Plasmid) |
| Size of vector backbone | 3.0 kb |
| Selectable markers | Am^r |
| Depositor | Kanemaki, Masato | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB13927 | pMK284 (Stag-FLAG-Hygro) | DNA solution |
Electronic file
Featured content
| Featured content | Auxin Inducible Degron (AID) System (English text) |
|---|---|
| Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
| Featured content | Genome Editing (English text) |
| Featured content | Genome Editing (Japanese text) |
| Featured content | Empty Backbone (English text) |
| Featured content | Empty Backbone (Japanese text) |
References
| original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB13927_A6B8p1_v2.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: M13_-40 | RDB13927_A6B8a_2.seq |
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>D03070A11_A6B8_4_M13(-40)_E02_14.ab1 1 CGCCATGCGC GCGCGTATAC GACTCACTAT AGGGCGAATT GGAGCTCGGA TCCGGTGCAG 61 GCGCCAAGGA AACCGCCGCT GCAAAATTCG AACGCCAGCA CATGGACAGC CGATCCCGGG 121 CTGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG 181 ATGACAAGTA GTGTACAAGT AATCTAGATA ACTGATCATA ATCAGCCATA CCACATTTGT 241 AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA AACATAAAAT 301 GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA 361 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC 421 CAAACTCATC AATGTATCTT AACGCGTCGA TCATATTCAA TAACCCTTAA TATAACTTCG 481 TATAATGTAT GCTATACGAA GTTATTAGGT CTGAAGAGGA GTTTACGTCC AGCCAAGCTT 541 AGGATCTCGA CCTCGAAATT CTACCGGGTA GGGGAGGCGC TTTTCCCAAG GCAGTCTGGA 601 GCATGCGCTT TAGCAGCCCC GCTGGGCACT TGGCGCTACA CAAGTGGCCT CTGGCCTCGC 661 ACACATTCCA CATCCACCGG TAGGCGCCAA CCGACTCCGT TCTTTGGTGG CCCCTTCGCG 721 CCACCTTCTA CTCCTCCCCT AGTCAGGAAG TTCCCCCCCG CCCCGCAGCT CGCGTCGTGC 781 AGGACGTGAC AAATGGAAGT AGCACGTCTC ACTAGTCTCG TGCAGATGGA CAGCACCGCT 841 GAGCAATGGA AGCGGGTAGG CCTTTGGGGC AGCGGCCAAT AGCAGCTTTG CTCCTTCGCT 901 TTCTGGGCTC AGAGGCTGGG AAGGGGTGGG TCCCGGGGCG GGCTCACGGG CGGGCTCAGG 961 GCGGGGCGGG CGCCCGAAAG TCCTCCCGGA GGCCCGGCAT TCTGCACGCT TCAAAGCGCA 1021 CGTCTGCCGG CGCTGTTCTC CTCTTCCTCA TCTCCAGGGC CCTTTCGACC CTGCCATCAA 1081 TCCTAGATCT CGGAGGCAGC TGAAGGCTAA CGCTTAGGCA TGCATAGATC GGAAGTCGTT 1141 GACTTCACGC GACGTCTGTC CGAGAAAAGG ACTACCATGG TA // |
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| Primer: PGKpro_F | RDB13927_A6B8b_2.seq |
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>D03070A11_A6B8_4_PGK-proF_F02_17.ab1 1 AGCGGGCGTA TCTCTCTTCT CATCTCCGGG CCTTTCGACC TGCATCCATC TAGATCTCGA 61 GCAGCTGAAG CTTACCGCTA GCATGGATAG ATCCGGAAAG CCTGAACTCA CCGCGACGTC 121 TGTCGAGAAG TTTCTGATCG AAAAGTTCGA CAGCGTCTCC GACCTGATGC AGCTCTCGGA 181 GGGCGAAGAA TCTCGTGCTT TCAGCTTCGA TGTAGGAGGG CGTGGATATG TCCTGCGGGT 241 AAATAGCTGC GCCGATGGTT TCTACAAAGA TCGTTATGTT TATCGGCACT TTGCATCGGC 301 CGCGCTCCCG ATTCCGGAAG TGCTTGACAT TGGGGAATTC AGCGAGAGCC TGACCTATTG 361 CATCTCCCGC CGTGCACAGG GTGTCACGTT GCAAGACCTG CCTGAAACCG AACTGCCCGC 421 TGTTCTGCAG CCGGTCGCGG AGGCCATGGA TGCGATCGCT GCGGCCGATC TTAGCCAGAC 481 GAGCGGGTTC GGCCCATTCG GACCGCAAGG AATCGGTCAA TACACTACAT GGCGTGATTT 541 CATATGCGCG ATTGCTGATC CCCATGTGTA TCACTGGCAA ACTGTGATGG ACGACACCGT 601 CAGTGCGTCC GTCGCGCAGG CTCTCGATGA GCTGATGCTT TGGGCCGAGG ACTGCCCCGA 661 AGTCCGGCAC CTCGTGCACG CGGATTTCGG CTCCAACAAT GTCCTGACGG ACAATGGCCG 721 CATAACAGCG GTCATTGACT GGAGCGAGGC GATGTTCGGG GATTCCCAAT ACGAGGTCGC 781 CAACATCTTC TTCTGGAGGC CGTGGTTGGC TTGTATGGAG CAGCAGACGC GCTACTTCGA 841 GCGGAGGCAT CCGGAGCTTG CAGGATCGCC GCGGCTCCGG GCGTATATGC TCCGCATTGG 901 TCTTGACCAA CTCTATCAGA GCTTGGTTGA CGGCAATTTC GATGATGCAG CTTGGGCGCA 961 GGGTCGATGC GACGCAATCG TCCCGATCCC GGAGCCCGGG ACTGTCGGCC GTACACAAAT 1021 CGCCCCGCAG AAGCGGCGGG CCCGTCCTGG ACCGATGGCC TGTGTAGAAG GTACTCGGCC 1081 GATAGTGGAA CCCGGAACGC CCAGCACTTC GTTCGAAGGG CAAAGGAAAT ACGGCTTAAG 1141 GCCCGTCGGC // |
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| Primer: M13R | RDB13927_A6B8c_2.seq |
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>D03070A11_A6B8_4_M13R_D02_11.ab1 1 CTCGCGCATT ACCTCACTAA AGGGACAAAA GCTGGGTACC GGATCCTAGT GAACCTCTTC 61 GAGGGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG GGTTCCGGAT 121 CTCGACCAGC TTCTGATGGA ATTAGAACTT GGCAAAACAA TACTGAGAAT GAAGTGTATG 181 TGGAACAGAG GCTGCTGATC TCGTTCTTCA GGCTATGAAA CTGACACATT TGGAAACCAC 241 AGTACTTAGA ACCACAAAGT GGGAATCAAG AGAAAAACAA TGATCCCACG AGAGATCTAT 301 AGATCTATAG ATCATGAGTG GGAGGAATGA GCTGGCCCTT AATTTGGTTT TGCTTGTTTA 361 AATTATGATA TCCAACTATG AAACATTATC ATAAAGCAAT AGTAAAGAGC CTTCAGTAAA 421 GAGCAGGCAT TTATCTAATC CCACCCCACC CCCACCCCCG TAGCTCCAAT CCTTCCATTC 481 AAAATGTAGG TACTCTGTTC TCACCCTTCT TAACAAAGTA TGACAGGAAA AACTTCCATT 541 TTAGTGGACA TCTTTATTGT TTAATAGATC ATCAATTTCT GCATCCCGGG GATCTGATAT 601 CATCGATGCA TGGGGTCGTG CGCTCCTTTC GGTCGGGCGC TGCGGGTCGT GGGGCGGGCG 661 GCTAGCCTAT TCCTTTGCCC TCGGACGAGT GCTGGGGCGT CGGTTTCCAC TATCGGCGAG 721 TACTTCTACA CAGCCATCGG TCCAGACGGC CGCGCTTCTG CGGGCGATTT GTGTACGCCC 781 GACAGTCCCG GCTCCGGATC GGACGATTGC GTCGCATCGA CACTGCGCCC AAGCTGCATC 841 ATCGAAATTG CCGTCAACCA AGCTCTGATA GAGTTGGTCA AGACCAATGC GGAGCATATA 901 CGCCCGGAGC CGCGGCGATC CTGCAAGCTC CGGATGCCTC CGCTCGAAGT AGCGCGTCTG 961 CTGCTCCATA CAAGCCAACC CACGGCCCTC CAGAGAGATG TTTGGGCGAC CCTCGTAATT 1021 GGGAAATCCC CCGACATCGC CCTCGCCTCA GTTCATTGAA CCGGCTTGCT ATGCGGTCCA 1081 TGATCAGTCA GGCACCATTG ATTTGGAAGC CGGAAAATTC CCGGACG // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. |
|---|
2018.12.11
GNP_filter3_RDBDEP_html_181012.pl