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pMK284 (Stag-FLAG-Hygro) (#RDB13927)

A plasmid for construction of C-terminal Stag-3FLAG tagging donors.

Alternative name Stag-FLAG-Hygro
Clone info. A plasmid for construction of C-terminal Stag-3FLAG tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone.
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Depositor|Developer Kanemaki, Masato |
 

Distribution information

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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。

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RDB13927 pMK284 (Stag-FLAG-Hygro) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMK284 (Stag-FLAG-Hygro) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13927).

Reference section:

Natsume, T., Kiyomitsu, T., Saga, Y., Kanemaki, M.T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13927_A6B8p1_v2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB13927_A6B8a_2.seq check
>D03070A11_A6B8_4_M13(-40)_E02_14.ab1
    1 CGCCATGCGC GCGCGTATAC GACTCACTAT AGGGCGAATT GGAGCTCGGA TCCGGTGCAG
   61 GCGCCAAGGA AACCGCCGCT GCAAAATTCG AACGCCAGCA CATGGACAGC CGATCCCGGG
  121 CTGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG
  181 ATGACAAGTA GTGTACAAGT AATCTAGATA ACTGATCATA ATCAGCCATA CCACATTTGT
  241 AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA AACATAAAAT
  301 GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA
  361 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC
  421 CAAACTCATC AATGTATCTT AACGCGTCGA TCATATTCAA TAACCCTTAA TATAACTTCG
  481 TATAATGTAT GCTATACGAA GTTATTAGGT CTGAAGAGGA GTTTACGTCC AGCCAAGCTT
  541 AGGATCTCGA CCTCGAAATT CTACCGGGTA GGGGAGGCGC TTTTCCCAAG GCAGTCTGGA
  601 GCATGCGCTT TAGCAGCCCC GCTGGGCACT TGGCGCTACA CAAGTGGCCT CTGGCCTCGC
  661 ACACATTCCA CATCCACCGG TAGGCGCCAA CCGACTCCGT TCTTTGGTGG CCCCTTCGCG
  721 CCACCTTCTA CTCCTCCCCT AGTCAGGAAG TTCCCCCCCG CCCCGCAGCT CGCGTCGTGC
  781 AGGACGTGAC AAATGGAAGT AGCACGTCTC ACTAGTCTCG TGCAGATGGA CAGCACCGCT
  841 GAGCAATGGA AGCGGGTAGG CCTTTGGGGC AGCGGCCAAT AGCAGCTTTG CTCCTTCGCT
  901 TTCTGGGCTC AGAGGCTGGG AAGGGGTGGG TCCCGGGGCG GGCTCACGGG CGGGCTCAGG
  961 GCGGGGCGGG CGCCCGAAAG TCCTCCCGGA GGCCCGGCAT TCTGCACGCT TCAAAGCGCA
 1021 CGTCTGCCGG CGCTGTTCTC CTCTTCCTCA TCTCCAGGGC CCTTTCGACC CTGCCATCAA
 1081 TCCTAGATCT CGGAGGCAGC TGAAGGCTAA CGCTTAGGCA TGCATAGATC GGAAGTCGTT
1141 GACTTCACGC GACGTCTGTC CGAGAAAAGG ACTACCATGG TA
//
Primer: PGKpro_F
Sequence file: RDB13927_A6B8b_2.seq check
>D03070A11_A6B8_4_PGK-proF_F02_17.ab1
    1 AGCGGGCGTA TCTCTCTTCT CATCTCCGGG CCTTTCGACC TGCATCCATC TAGATCTCGA
   61 GCAGCTGAAG CTTACCGCTA GCATGGATAG ATCCGGAAAG CCTGAACTCA CCGCGACGTC
  121 TGTCGAGAAG TTTCTGATCG AAAAGTTCGA CAGCGTCTCC GACCTGATGC AGCTCTCGGA
  181 GGGCGAAGAA TCTCGTGCTT TCAGCTTCGA TGTAGGAGGG CGTGGATATG TCCTGCGGGT
  241 AAATAGCTGC GCCGATGGTT TCTACAAAGA TCGTTATGTT TATCGGCACT TTGCATCGGC
  301 CGCGCTCCCG ATTCCGGAAG TGCTTGACAT TGGGGAATTC AGCGAGAGCC TGACCTATTG
  361 CATCTCCCGC CGTGCACAGG GTGTCACGTT GCAAGACCTG CCTGAAACCG AACTGCCCGC
  421 TGTTCTGCAG CCGGTCGCGG AGGCCATGGA TGCGATCGCT GCGGCCGATC TTAGCCAGAC
  481 GAGCGGGTTC GGCCCATTCG GACCGCAAGG AATCGGTCAA TACACTACAT GGCGTGATTT
  541 CATATGCGCG ATTGCTGATC CCCATGTGTA TCACTGGCAA ACTGTGATGG ACGACACCGT
  601 CAGTGCGTCC GTCGCGCAGG CTCTCGATGA GCTGATGCTT TGGGCCGAGG ACTGCCCCGA
  661 AGTCCGGCAC CTCGTGCACG CGGATTTCGG CTCCAACAAT GTCCTGACGG ACAATGGCCG
  721 CATAACAGCG GTCATTGACT GGAGCGAGGC GATGTTCGGG GATTCCCAAT ACGAGGTCGC
  781 CAACATCTTC TTCTGGAGGC CGTGGTTGGC TTGTATGGAG CAGCAGACGC GCTACTTCGA
  841 GCGGAGGCAT CCGGAGCTTG CAGGATCGCC GCGGCTCCGG GCGTATATGC TCCGCATTGG
  901 TCTTGACCAA CTCTATCAGA GCTTGGTTGA CGGCAATTTC GATGATGCAG CTTGGGCGCA
  961 GGGTCGATGC GACGCAATCG TCCCGATCCC GGAGCCCGGG ACTGTCGGCC GTACACAAAT
 1021 CGCCCCGCAG AAGCGGCGGG CCCGTCCTGG ACCGATGGCC TGTGTAGAAG GTACTCGGCC
 1081 GATAGTGGAA CCCGGAACGC CCAGCACTTC GTTCGAAGGG CAAAGGAAAT ACGGCTTAAG
1141 GCCCGTCGGC
//
Primer: M13R
Sequence file: RDB13927_A6B8c_2.seq check
>D03070A11_A6B8_4_M13R_D02_11.ab1
    1 CTCGCGCATT ACCTCACTAA AGGGACAAAA GCTGGGTACC GGATCCTAGT GAACCTCTTC
   61 GAGGGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG GGTTCCGGAT
  121 CTCGACCAGC TTCTGATGGA ATTAGAACTT GGCAAAACAA TACTGAGAAT GAAGTGTATG
  181 TGGAACAGAG GCTGCTGATC TCGTTCTTCA GGCTATGAAA CTGACACATT TGGAAACCAC
  241 AGTACTTAGA ACCACAAAGT GGGAATCAAG AGAAAAACAA TGATCCCACG AGAGATCTAT
  301 AGATCTATAG ATCATGAGTG GGAGGAATGA GCTGGCCCTT AATTTGGTTT TGCTTGTTTA
  361 AATTATGATA TCCAACTATG AAACATTATC ATAAAGCAAT AGTAAAGAGC CTTCAGTAAA
  421 GAGCAGGCAT TTATCTAATC CCACCCCACC CCCACCCCCG TAGCTCCAAT CCTTCCATTC
  481 AAAATGTAGG TACTCTGTTC TCACCCTTCT TAACAAAGTA TGACAGGAAA AACTTCCATT
  541 TTAGTGGACA TCTTTATTGT TTAATAGATC ATCAATTTCT GCATCCCGGG GATCTGATAT
  601 CATCGATGCA TGGGGTCGTG CGCTCCTTTC GGTCGGGCGC TGCGGGTCGT GGGGCGGGCG
  661 GCTAGCCTAT TCCTTTGCCC TCGGACGAGT GCTGGGGCGT CGGTTTCCAC TATCGGCGAG
  721 TACTTCTACA CAGCCATCGG TCCAGACGGC CGCGCTTCTG CGGGCGATTT GTGTACGCCC
  781 GACAGTCCCG GCTCCGGATC GGACGATTGC GTCGCATCGA CACTGCGCCC AAGCTGCATC
  841 ATCGAAATTG CCGTCAACCA AGCTCTGATA GAGTTGGTCA AGACCAATGC GGAGCATATA
  901 CGCCCGGAGC CGCGGCGATC CTGCAAGCTC CGGATGCCTC CGCTCGAAGT AGCGCGTCTG
  961 CTGCTCCATA CAAGCCAACC CACGGCCCTC CAGAGAGATG TTTGGGCGAC CCTCGTAATT
 1021 GGGAAATCCC CCGACATCGC CCTCGCCTCA GTTCATTGAA CCGGCTTGCT ATGCGGTCCA
1081 TGATCAGTCA GGCACCATTG ATTTGGAAGC CGGAAAATTC CCGGACG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP]