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Hiroshi Sasaki's web page in English / in Japanese
| Catalog # | Name of clone | Running title | Publication |
| RDB20709 | pT2K-BI-TRE-HA-Lats2/EGFP | Bidirectional expression vector of mouse Lats2 (HA-tag at N-terminus) and EGFP. Tet-inducible. Tol2 transposon vector. | Hirono, N., Fate specification triggers a positive feedback loop of TEAD-YAP and NANOG to promote epiblast formation in preimplantation embryos. Development 152 (1): dev203091 (2025). PMID 39629521. (original). [link to RRC of NBRP] |
| RDB12216 | E-Cadherin-delta C-Flag/pCAG | Expression clone of mouse E-cadherin, C-terminal deletion | Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original). [link to RRC of NBRP] |
| RDB12215 | Ecad-Flag/pCAG | Expression clone of mouse E-cadherin | Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original). [link to RRC of NBRP] |
| RDB12214 | pCMV/SV-Flag-Lats2-KD | Expression clone of mouse Lats2, kinase dead variant | Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original). [link to RRC of NBRP] |
| RDB12213 | pCMV/SV-Flag-Lats2 | Expression clone of mouse Lats2 | Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original). [link to RRC of NBRP] |
| RDB12212 | HA-Amot-delta(101-141)/pcDNA3.1-pA83 | Expression clone of mouse Amot (p130), lacking 101-141aa | Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original). [link to RRC of NBRP] |
| RDB12211 | HA-Amot-delta(45-100)/pcDNA3.1-pA83 | Expression clone of mouse Amot (p130), lacking 45-100aa | Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original). [link to RRC of NBRP] |
| RDB12210 | HA-Merlin/pCAG-pA83 | Expression clone of mouse Merlin | Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original). [link to RRC of NBRP] |
| RDB12209 | HA-Amot130-S176E/pcDNA3.1-pA83 | Expression clone of mouse Amot (p130), S176E mutant | Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original). [link to RRC of NBRP] |
| RDB12208 | HA-Amot130-S176A/pcDNA3.1-pA83 | Expression clone of mouse Amot (p130), S176A mutant | Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original). [link to RRC of NBRP] |
| RDB12207 | HA-Amot130-delta PDZbd/pcDNA3.1-pA83 | Expression clone of mouse Amot (p130), deletion of PDZbd | Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original). [link to RRC of NBRP] |
| RDB12206 | HA-Amot130-m5/pcDNA3.1-pA83 | Expression clone of mouse Amot (p130), mPY123 mutation | Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original). [link to RRC of NBRP] |
| RDB12205 | HA-Amot130-delta CC/pcDNA3.1-pA83 | Expression clone of mouse Amot (p130), deletion of CC domain | Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original). [link to RRC of NBRP] |
| RDB12204 | HA-Amot80/pcDNA3.1-pA83 | Expression clone of mouse Amot (p80) | Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original). [link to RRC of NBRP] |
| RDB12203 | HA-Amot130/pcDNA3.1-pA83 | Expression clone of mouse Amot (p130) | Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original). [link to RRC of NBRP] |
| RDB12202 | pcDNA3.1-pA83-aPKC lambda KD | Expression clone of PKC lambda, KD form | Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original). [link to RRC of NBRP] |
| RDB12201 | pcDNA3.1-pA83-Lats2KD | Expression clone of mouse Lats2 cDNA, kinase dead variant | Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original). [link to RRC of NBRP] |
| RDB12200 | pcDNA3.1-pA83-Lats2 | Expression clone of mouse Lats2 cDNA | Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original). [link to RRC of NBRP] |
| RDB12199 | pcDNA3.1-pA83-NDR1 | Expression clone of mouse Ndr1 | Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original). [link to RRC of NBRP] |
| RDB12198 | pG4-TK-Luc | Reporter vector of transcripional regulation | Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original). [link to RRC of NBRP] |
| RDB12197 | pCMV-Gal4-Tead4C | Expression clone of C-terminal portion of mouse Tead4, fused with Gal4 DBD | Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original). [link to RRC of NBRP] |
| RDB12196 | pCMV-Gal4(BD) | Effector vector for testing transcriptional regulation, Gal4 DNA binding domain | Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original). [link to RRC of NBRP] |
| RDB12195 | pcDNA3.1-pA83-dnYap | Expression clone of mouse Yap1, dominant negative | Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original). [link to RRC of NBRP] |
| RDB12194 | pcDNA3.1-pA83-HA-Yap-S112A | Expression clone of mouse Yap1, S112A mutation | Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original). [link to RRC of NBRP] |
| RDB12193 | pcDNA3.1-pA83-HA-TAZ | Expression clone of mouse TAZ | Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original). [link to RRC of NBRP] |
| RDB12192 | pcDNA3.1-pA83-HA-Yap-delta AD | Expression clone of mouse Yap1, deletion of AD | Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original). [link to RRC of NBRP] |
| RDB12191 | pcDNA3.1-pA83-HA-Yap-delta TeadBD | Expression clone of mouse Yap1, deletion of Tead BD | Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original). [link to RRC of NBRP] |
| RDB12190 | pcDNA3.1-pA83-HA-Yap | Expression clone of mouse Yap1 | Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original). [link to RRC of NBRP] |
| RDB12189 | pcDNA3.1-pA83-Ssdp1 | Expression clone of mouse Ssdp1 | Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original). [link to RRC of NBRP] |
| RDB12188 | pcDNA3.1-pA83-Tead1VP16 | Expression clone of mouse Tead1, constitutive active | Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original). [link to RRC of NBRP] |
| RDB12187 | pcDNA3.1-pA83-Tead4EnR | Expression clone of mouse Tead4, repressor-modified form | Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original). [link to RRC of NBRP] |
| RDB12186 | pcDNA3.1-pA83-Tead4VP16 | Expression clone of mouse Tead4, constitutive active | Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original). [link to RRC of NBRP] |
| RDB12185 | pcDNA3.1-pA83-Tead4 | Expression clone of mouse Tead4 | Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original). [link to RRC of NBRP] |
| RDB12183 | pcDNA3.1-pA83-beta-globin | Expression clone of Xenopus laevis beta-globin | Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original). [link to RRC of NBRP] |
| RDB12182 | pMYs-HA-dnYap1-IRES-EGFP | Retroviral vector of mouse Yap1, dominant negative | Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. (original). [link to RRC of NBRP] |
| RDB12181 | pMYs-HA-Yap1 S112A-IRES-EGFP | Retroviral vector of mouse Yap1, S112A mutant | Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. (original). [link to RRC of NBRP] |
| RDB12180 | pMYs-HA-Yap1 DAD-IRES-EGFP | Retroviral vector of mouse Yap1, deletion of AD | Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. (original). [link to RRC of NBRP] |
| RDB12179 | pMYs-HA-Yap1 DWW-IRES-EGFP | Retroviral vector of mouse Yap1, deletion of WW domain | Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. (original). [link to RRC of NBRP] |
| RDB12178 | pMYs-HA-Yap1 DTead BD-IRES-EGFP | Retroviral vector of mouse Yap1, deletion of Tead BD | Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. (original). [link to RRC of NBRP] |
| RDB12177 | pMYs-HA-Yap1-IRES-EGFP | Retroviral vector of mouse Yap1 | Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. (original). [link to RRC of NBRP] |
| RDB12176 | pMYs-Tead4-VP16-IRES-EGFP | Retroviral vector of mouse Tead4, constitutive active | Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. (original). [link to RRC of NBRP] |
| RDB12175 | pMYs-Tead2-EnR-IRES-EGFP | Retroviral vector of mouse Tead2, dominant negative | Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. (original). [link to RRC of NBRP] |
| RDB12174 | pMYs-Tead2-VP16-IRES-EGFP | Retroviral vector of mouse Tead2, constitutive active | Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. (original). [link to RRC of NBRP] |
| RDB12173 | pMYs-Tead2FL-IRES-EGFP | Retroviral vector of mouse Tead2 | Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. (original). [link to RRC of NBRP] |
| RDB12172 | pMYs-Tead1-VP16-IRES-EGFP | Retroviral vector of mouse Tead1, constitutive active | Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. (original). [link to RRC of NBRP] |
| RDB12171 | pCMV-Flag-Tead2 | Expression clone of mouse Tead2, FLAG-tag | Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. (original). [link to RRC of NBRP] |
| RDB12170 | pCMV-Flag-Tead1 | Expression clone of mouse Tead1, FLAG-tag | Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. (original). [link to RRC of NBRP] |
| RDB08068 | delta51-LucII | Reporter plasmid of chicken delta-crystallin basal promoter | Kamachi, Y., Overlapping positive and negative regulatory elements determine lens-specific activity of the delta 1-crystallin enhancer. Mol. Cell. Biol., 13 (9): 5206-5215 (1993). PMID 8355679. (original). [link to RRC of NBRP] |
| RDB08067 | 8xGT-IIC-LucII | Reporter plasmid carring eight copies of Tead-binding motif | Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. (original). [link to RRC of NBRP] |
| RDB08066 | pcDNA3.1-His-hGLI3 | Expression vector of human Gli3 cDNA | Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919. (original). [link to RRC of NBRP] |
| RDB08065 | pcDNA3.1-His-Gli2 | Expression vector of mouse Gli2 cDNA | Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919. (original). [link to RRC of NBRP] |
| RDB08064 | pcDNA3.1-His-Gli1 | Expression vector of mouse Gli1 cDNA | Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919. (original). [link to RRC of NBRP] |
| RDB08063 | pcDNA3.1-His-hGLI1 | Expression vector of human Gli1 cDNA | Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919. (original). [link to RRC of NBRP] |
| RDB08062 | 8xm3'Gli-BS-delta51-LucII | Reporter plasmid carring eight copies of mutated Gli-binding site | Sasaki, H., A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development, 124 (7): 1313-1322 (1997). PMID 9118802. (original). [link to RRC of NBRP] |
| RDB08061 | 8x3'Gli-BS-delta51-LucII | Reporter plasmid carring eight copies of Gli-binding site | Sasaki, H., A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development, 124 (7): 1313-1322 (1997). PMID 9118802. (original). [link to RRC of NBRP] |
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