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Hiroshi Sasaki's Resource

Hiroshi Sasaki's web page in English    in Japanese.

Learn more about their research in Reseachmap[link]   in English     in Japanese.

Catalog #Name of cloneRunning titlePublication
RDB080618x3'Gli-BS-delta51-LucIIReporter plasmid carring eight copies of Gli-binding siteSasaki, H., A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development, 124 (7): 1313-1322 (1997). PMID 9118802. (original).
RDB080628xm3'Gli-BS-delta51-LucIIReporter plasmid carring eight copies of mutated Gli-binding siteSasaki, H., A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development, 124 (7): 1313-1322 (1997). PMID 9118802. (original).
RDB08063pcDNA3.1-His-hGLI1Expression vector of human Gli1 cDNASasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919. (original).
RDB08064pcDNA3.1-His-Gli1Expression vector of mouse Gli1 cDNASasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919. (original).
RDB08065pcDNA3.1-His-Gli2Expression vector of mouse Gli2 cDNASasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919. (original).
RDB08066pcDNA3.1-His-hGLI3Expression vector of human Gli3 cDNASasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919. (original).
RDB080678xGT-IIC-LucIIReporter plasmid carring eight copies of Tead-binding motifOta, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. (original).
RDB08068delta51-LucIIReporter plasmid of chicken delta-crystallin basal promoterKamachi, Y., Overlapping positive and negative regulatory elements determine lens-specific activity of the delta 1-crystallin enhancer. Mol. Cell. Biol., 13 (9): 5206-5215 (1993). PMID 8355679. (original).
RDB12170pCMV-Flag-Tead1Expression clone of mouse Tead1, FLAG-tagOta, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856. (original).
RDB12171pCMV-Flag-Tead2Expression clone of mouse Tead2, FLAG-tagOta, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856. (original).
RDB12172pMYs-Tead1-VP16-IRES-EGFPRetroviral vector of mouse Tead1, constitutive activeOta, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856. (original).
RDB12173pMYs-Tead2FL-IRES-EGFPRetroviral vector of mouse Tead2Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856. (original).
RDB12174pMYs-Tead2-VP16-IRES-EGFPRetroviral vector of mouse Tead2, constitutive activeOta, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856. (original).
RDB12175pMYs-Tead2-EnR-IRES-EGFPRetroviral vector of mouse Tead2, dominant negativeOta, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856. (original).
RDB12176pMYs-Tead4-VP16-IRES-EGFPRetroviral vector of mouse Tead4, constitutive activeOta, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856. (original).
RDB12177pMYs-HA-Yap1-IRES-EGFPRetroviral vector of mouse Yap1Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856. (original).
RDB12178pMYs-HA-Yap1 DTead BD-IRES-EGFPRetroviral vector of mouse Yap1, deletion of Tead BDOta, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856. (original).
RDB12179pMYs-HA-Yap1 DWW-IRES-EGFPRetroviral vector of mouse Yap1, deletion of WW domainOta, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856. (original).
RDB12180pMYs-HA-Yap1 DAD-IRES-EGFPRetroviral vector of mouse Yap1, deletion of ADOta, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856. (original).
RDB12181pMYs-HA-Yap1 S112A-IRES-EGFPRetroviral vector of mouse Yap1, S112A mutantOta, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856. (original).
RDB12182pMYs-HA-dnYap1-IRES-EGFPRetroviral vector of mouse Yap1, dominant negativeOta, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856. (original).
RDB12183pcDNA3.1-pA83-beta-globinExpression clone of Xenopus laevis beta-globinNishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original).
RDB12185pcDNA3.1-pA83-Tead4Expression clone of mouse Tead4Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original).
RDB12186pcDNA3.1-pA83-Tead4VP16Expression clone of mouse Tead4, constitutive activeNishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original).
RDB12187pcDNA3.1-pA83-Tead4EnRExpression clone of mouse Tead4, repressor-modified formNishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original).
RDB12188pcDNA3.1-pA83-Tead1VP16Expression clone of mouse Tead1, constitutive activeNishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original).
RDB12189pcDNA3.1-pA83-Ssdp1Expression clone of mouse Ssdp1Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original).
RDB12190pcDNA3.1-pA83-HA-YapExpression clone of mouse Yap1Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original).
RDB12191pcDNA3.1-pA83-HA-Yap-delta TeadBDExpression clone of mouse Yap1, deletion of Tead BDNishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original).
RDB12192pcDNA3.1-pA83-HA-Yap-delta ADExpression clone of mouse Yap1, deletion of ADNishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original).
RDB12193pcDNA3.1-pA83-HA-TAZExpression clone of mouse TAZNishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original).
RDB12194pcDNA3.1-pA83-HA-Yap-S112AExpression clone of mouse Yap1, S112A mutationNishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original).
RDB12195pcDNA3.1-pA83-dnYapExpression clone of mouse Yap1, dominant negativeNishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original).
RDB12196pCMV-Gal4(BD)Effector vector for testing transcriptional regulation, Gal4 DNA binding domainNishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original).
RDB12197pCMV-Gal4-Tead4CExpression clone of C-terminal portion of mouse Tead4, fused with Gal4 DBDNishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original).
RDB12198pG4-TK-LucReporter vector of transcripional regulationNishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original).
RDB12199pcDNA3.1-pA83-NDR1Expression clone of mouse Ndr1Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original).
RDB12200pcDNA3.1-pA83-Lats2Expression clone of mouse Lats2 cDNANishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original).
RDB12201pcDNA3.1-pA83-Lats2KDExpression clone of mouse Lats2 cDNA, kinase dead variantNishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. (original).
RDB12202pcDNA3.1-pA83-aPKC lambda KDExpression clone of PKC lambda, KD formHirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original).
RDB12203HA-Amot130/pcDNA3.1-pA83Expression clone of mouse Amot (p130)Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original).
RDB12204HA-Amot80/pcDNA3.1-pA83Expression clone of mouse Amot (p80)Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original).
RDB12205HA-Amot130-delta CC/pcDNA3.1-pA83Expression clone of mouse Amot (p130), deletion of CC domainHirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original).
RDB12206HA-Amot130-m5/pcDNA3.1-pA83Expression clone of mouse Amot (p130), mPY123 mutationHirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original).
RDB12207HA-Amot130-delta PDZbd/pcDNA3.1-pA83Expression clone of mouse Amot (p130), deletion of PDZbdHirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original).
RDB12208HA-Amot130-S176A/pcDNA3.1-pA83Expression clone of mouse Amot (p130), S176A mutantHirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original).
RDB12209HA-Amot130-S176E/pcDNA3.1-pA83Expression clone of mouse Amot (p130), S176E mutantHirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original).
RDB12210HA-Merlin/pCAG-pA83Expression clone of mouse MerlinHirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original).
RDB12211HA-Amot-delta(45-100)/pcDNA3.1-pA83Expression clone of mouse Amot (p130), lacking 45-100aaHirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original).
RDB12212HA-Amot-delta(101-141)/pcDNA3.1-pA83Expression clone of mouse Amot (p130), lacking 101-141aaHirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original).
RDB12213pCMV/SV-Flag-Lats2Expression clone of mouse Lats2Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original).
RDB12214pCMV/SV-Flag-Lats2-KDExpression clone of mouse Lats2, kinase dead variantHirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original).
RDB12215Ecad-Flag/pCAGExpression clone of mouse E-cadherinHirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original).
RDB12216E-Cadherin-delta C-Flag/pCAGExpression clone of mouse E-cadherin, C-terminal deletionHirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. (original).

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2019.09.19

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