Resource data sheet
Prev.

pcDNA3.1-pA83-dnYap

Expression clone of mouse Yap1, dominant negative

Catalog number RDB12195
Resource name pcDNA3.1-pA83-dnYap
Clone info. Expression clone of mouse Yap1, dominant negative. cDNA fragment 210 to 1,056 of NM_009534.3 (CDS, 207..1625) is cloned. S112A mutation, deletion of activation domain, Engrailed repression domain are constructed.
See Fig. 5A of Nishioka et al., Dev. Cell 16: 398-410 (2009). The expression vector has a stretch of poly(A). Known differences: G to C substitution at 212 nt (Glu to Asp at 2nd aa). C to A substitution at 477 nt (Arg at 91st aa, synonymous) and XhoI site insertion at the position.
Vector backbone pcDNA3.1-pA83 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Mouse Yap1
Depositor Sasaki, Hiroshi |
 
Other clones in our bank mouse Yap1 (NCBI Gene 22601) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12195 pcDNA3.1-pA83-dnYap DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12195_A4Dlp1_v2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB12195_A4Dla.seq
>12195_12195_A4Dl_CMV-Forward_E04_13.ab1
    1 CGTCAACTCC GCCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
  121 ACTCACTATA GGGAGACCCA AGCTGGCTAG TTAAGCTTGC CGCCACCATG TACCCATACG
  181 ACGTTCCAGA TTACGCTGGT ACCGACCCCG CGCAACAGCC GCCGCCCCAG CCGGCCCCGC
  241 AAGGCCCCGC GCCGCCGTCC GTGTCTCCGG CCGGGACCCC CGCGGCCCCG CCCGCACCCC
  301 CGGCCGGCCA CCAGGTCGTG CACGTCCGCG GGGACTCGGA GACCGACTTG GAGGCGCTCT
  361 TCAATGCCGT CATGAACCCC AAGACGGCCA ACGTGCCTCA GACCGTGCCC ATGCGGCTTC
  421 GCAAGCTGCC CGACTCCTTC TTCAAGCCGC CTGAGCCCAA GTCCCACTCG AGACAGGCCA
  481 GTACTGATGC AGGTACTGCG GGAGCTCTGA CTCCACAGCA TGTTCGAGCT CACGCATCTC
  541 CAGCCTCCCT GCAGCTGGGT GCCGTTTCTC CTGGGACACT CACAGCCAGT GGCGTTGTCT
  601 CTGGCCCTGC CGCTGCCCCT GCAGCTCAGC ATCTCCGGCA GTCCTCCTTT GAGATCCCTG
  661 ATGATGTACC ACTGCCAGCA GGCTGGGAGA TGGCCAAGAC ATCTTCTGGT CAAAGATACT
  721 TCTTAAATCA CAACGATCAG ACAACAACAT GGCAGGACCC CCGGAAGGCC ATGCTTTCGC
  781 AACTGAACGT TCCTGCGCCT GCCAGCCCAG CGGTGCCCCA GACGCTGATG AATTCTGCCT
  841 CAGGACCTCT TCCTGATGGA TGGGAGCAAG CCATGACTCA GGATGGAGAA GTTTACTACA
  901 TAAACCATAA GAACAAGACC ACATCCTGGG CTGGACCCAA AGGCTGGACC CTCGTTTTGC
  961 CATGAACCAG AGGATCACTC AGAGTGCTCC AGTGAAGCAG CCCCCACCCT TTGGCTCCCC
 1021 AGAGCCCACA GGGAGGGCGT CCTGTGTGGA GGCAAGCTAG ATAAAGGAAA GCTTTCTCAC
 1081 GTGTAAATCG AATGACTGGC AGGATCGCTG CCAGGCCACA GGTCAGCGCC CCAAGCCCCC
1141 AATTAAGTCG A
//
Primer: GNP_primer#10 RDB12195_A4Dlb.seq
>12195_12195_A4Dl_GNP_primer+#10_F04_16.ab1
    1 TTATTTGTGC AATTCATTTA AAAGATAACG TTTACAGGTT CTTTGGTGCC CATAGTCGAG
   61 CGGCCGCGGA TCCCTAGTTC AGGTCCTCCT CGGAGATCAG CTTCTGCTCA AGCTTGGGCT
  121 GCATAGATCC CAGAGCAGAT TTCTCTGGAT TGGCATCGCT CATCTTGGAG CTCATGGGCG
  181 GCGGGATGAC GGCATTGGCT GCACTCCTGC GCAGGCGACT CTGCACGATT TCCTCGTGTA
  241 TCCTGGGATA GGCAGCCGGA TTGAAGCAGT TAAGGAAGTT GGCCCGTTCC AGCATCATGG
  301 CCGCAGTAGC GGCTGCGGCG GCAGCTTGCT GTTGCCGGCT AAACTCCAGC AGATCCACTC
  361 TTGTAAAGGC GGAGGGCGTG GCAGTCTGGG AGCGATTCGC CTCGAAGGGG CGGAATATGC
  421 TGGCCTGGTT CTCAATCGAC TTGCCAGGCT TCTGGACATC TCCGAAACGA TCGCTCAGGA
  481 TGTTGGAGAT GGAAAAGGCC AGCGAGGGCT TGGCCGTCGA CTGCTGCTGG TGGGCGGGTG
  541 GTGGCAGGCG TCCGCCCGCC GACGTATCAT CCACATCCAC ATCAATGTCG TCATCCTCCT
  601 CCGCCTCCTC CTCCTCGTTG GTGGTGTGCG TCTGATTGTG GAAACTCATG TCACCGATCA
  661 CGGAGTCGCT TTCCTCCTCC TTGATGGTCA GCGGCGTGGA CGAGGCGGGC GTGGAGCAGG
  721 ATGCGGGCGA TCCACTGCCA CTGAGGCGCT GCTGCAGTGC AGCAGCATGA GCGTGGGCGG
  781 CCGCAGCAGC AGCTGCAGCA GCGGCGGCTG CAGCGGCATC GAAGGCCATT GCCGGATGGT
  841 GGAAGACACC GGCGGCCAGT TGCTGTTGGT GCAACTGCTG CAGTTGCTGC AGCTGGTGGA
  901 GGTGCTGCAT TTGCTGCTGC TGTTGCTGCT GCTGCTGTTG CTGGTGGTGA AGATGCTGCA
  961 TTTGTAGGGT AATGGGGCTG GGCGCTGACT GTGGGCTGCA GCGATCCTCC AGGGCATCGA
 1021 TTAACCACGT GAAGAAAGCT TTTCTTTTAT CTAGCTTGCC CTCCCACCCC AGACGCCCTT
 1081 CCCTTGTGGG GCTCTGGGGA GCCACGGTGG GGGCTGCTTC CACTGGGAGG CCACCTTCTC
1141 TGGAGTGTGA
//
Primer: Yap-M_F RDB12195_A4Dlc.seq
>12195_12195_A4Dl_Yap-M_F_G08_20.ab1
    1 CCGGGTAGCA TGCTTTCGCA CTGACGTTCC TGCGCCTGCC AGCCCAGCGG TGCCCCAGAC
   61 GCTGATGAAT TCTGCCTCAG GACCTCTTCC TGATGGATGG GAGCAAGCCA TGACTCAGGA
  121 TGGAGAAGTT TACTACATAA ACCATAAGAA CAAGACCACA TCCTGGCTGG ACCCAAGGCT
  181 GGACCCTCGT TTTGCCATGA ACCAGAGGAT CACTCAGAGT GCTCCAGTGA AGCAGCCCCC
  241 ACCCTTGGCT CCCCAGAGCC CACAGGGAGG CGTCCTGGGT GGAGGCAAGC TAGATAAAGA
  301 AAGCTTTCTC ACGTGGTTAA TCGATGCCCT GGAGGATCGC TGCAGCCCAC AGTCAGCGCC
  361 CAGCCCCATT ACCCTACAAA TGCAGCATCT TCACCACCAG CAACAGCAGC AGCAGCAACA
  421 GCAGCAGCAA ATGCAGCACC TCCACCAGCT GCAGCAACTG CAGCAGTTGC ACCAACAGCA
  481 ACTGGCCGCC GGTGTCTTCC ACCATCCGGC AATGGCCTTC GATGCCGCTG CAGCCGCCGC
  541 TGCTGCAGCT GCTGCTGCGG CCGCCCACGC TCATGCTGCT GCACTGCAGC AGCGCCTCAG
  601 TGGCAGTGGA TCGCCCGCAT CCTGCTCCAC GCCCGCCTCG TCCACGCCGC TGACCATCAA
  661 GGAGGAGGAA AGCGACTCCG TGATCGGTGA CATGAGTTTC CACAATCAGA CGCACACCAC
  721 CAACGAGGAG GAGGAGGCGG AGGAGGATGA CGACATTGAT GTGGATGTGG ATGATACGTC
  781 GGCGGGCGGA CGCCTGCCAC CACCCGCCCA CCAGCAGCAG TCGACGGCCA AGCCCTCGCT
  841 GGCCTTTTCC ATCTCCAACA TCCTGAGCGA TCGTTTCGGA GATGTCCAGA AGCCTGGCAA
  901 GTCGATTGAG AACCAGGCCA GCATATTCCG CCCCTTCGAG GCGAATCGCT CCCAGACTGC
  961 CACGCCCTCC GCCTTTACAA GAGTGGATCT GCTGGGAGTT TAGCCCGGCA ACAGCAAGCT
 1021 GCCGCCGCAG CCGCTACTGC GGCCATGATG CTGGACGGGG CACTTTCCTT TAACTGCCTC
 1081 ATCTGCTGCT ATCCCAGATA CACGAGGAAT TCGTGCAGAG TCGCTGCGCA AGGAGTGCCA
1141 GCCAATGACC GATTCAATTC CTAC
//
Primer: BGH_rev RDB12195_A4Dld.seq
>12195_12195_A4Dl_BGHrev_E03_15.ab1
    1 CGTCTCATGG TGATGGTGAT GATGACCGGT ATGCATATTC AGATCCTCTT CTGAGATGAG
   61 TTTTTGTTCG AAGGGCCCTC TAGACTCGAG GTACGCCACA GCGTATGATG CGTATTTTTT
  121 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
  181 TTTTTTTCTT TCCCAAATTT TTTTTTGGGC CCTTTCATTT TAAAAAAAAA ACTTTTACGG
  241 GTTTTTTGGG GCCCAAAACC AAGGGGCCCG GAATCCCTTT TTCGGGCCCC CCCCGAAAAA
  301 CCCTTTTTGC CCAACTTGGG GGTGAAAAAA CCCCAAAAAA AATTTTCTGG GATGGGCCCC
  361 CCCCTTTTGG GACCCCAGGG GGGGGGGAAA AAAGGTTTTG GTTGCCCCCC GGGGAGGGGA
  421 ATTTTGAAAA ATTTCCCCGG GTTTCCGGGA AAAGGCCCCC GAATTAAACC ATTTAAGAAA
  481 TTTGCCCCTT CCCCCCTTCT GGGCCCCAAA ACCGGCGGGG GGGGCATTTT TTTTTTGCCG
  541 GTAAAACCCC AAAAAATCCC TTTTTTAAAA GGGGAAGGGG GGGGCATTTG GGAAAAAATT
  601 CCCCCCAAGG GGGGGAAAAT GGGGGCCGGT TTCCCACCCA TTTGCCGGGT TCGGGATTTC
  661 CCCAAACTTT CCCTCAGAAG GGGGAAAGGG AAAGCCCCGA GGCTTGGCCC TCACGGCTGT
  721 GGTGCGTGTG TGGAGGCTCC CCCCAATTTC TCCACTCCAC ATGATGTGTC ATCCCCCCCC
  781 CCCCTCCCCC CCTCGTGGGG TGTGCTCGAT TGGGAAACTC TGTCACATAT CGGAGTCTTT
  841 TCTTCTTCTT TGAGTCCCGG TGACGAGCGC TGAACAGTCC GCCATCCCTG CCTAGGCTGT
  901 TCATGCACTA GCTGGCGCAC AATGCAACCG GCTGAAGCTC AGCATGCTGA TGAATCGGCA
961 TCGTTGACGT CGATGCACTG AGTCATT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085.

2019.04.09

GNP_filter3_RDBDEP_html_190327.pl