Expression clone of mouse Yap1, dominant negative
Clone info. | Expression clone of mouse Yap1, dominant negative. cDNA fragment 210 to 1,056 of NM_009534.3 (CDS, 207..1625) is cloned. S112A mutation, deletion of activation domain, Engrailed repression domain are constructed. |
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Comment | See Fig. 5A of Nishioka et al., Dev. Cell 16: 398-410 (2009). HA-tag is fused before Yap1, and Myc-tag is fused after Engrailed repression domain. The expression vector has a stretch of poly(A). Known differences: G to C substitution at 212 nt (Glu to Asp at 2nd aa). C to A substitution at 477 nt (Arg at 91st aa, synonymous) and XhoI site insertion at the position. |
Vector backbone | pcDNA3.1-pA83 (Plasmid) |
Size of vector backbone | 5.4 kb |
Selectable markers | Amp^r |
Gene/insert name | Mouse Yap1 |
Depositor|Developer | Sasaki, Hiroshi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB12195 | pcDNA3.1-pA83-dnYap | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pcDNA3.1-pA83-dnYap was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12195). |
Reference section:
Nishioka, N., Inoue, K., Adachi, K., Kiyonari, H., Ota, M., Ralston, A., Yabuta, N., Hirahara, S., Stephenson, R.O., Ogonuki, N., Makita, R., Kurihara, H., Morin-Kensicki, E.M., Nojima, H., Rossant, J., Nakao, K., Niwa, H., Sasaki, H., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB12195_A3Icp1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: CMV_Forward (Pr0016) Region: HA tag, insert 5' Sequence file: RDB12195_A3Ica.seq ![]() |
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>12195_3_CMV_F_F11_17.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGGGCGG TAGGCGTGTA CGGTGGGAGG 61 TCTATATAAG CAGAGCTCTC TGGNNAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA 121 TTAATACGAC TCACTATAGG GAGACCCAAG CTGGCTAGTT AAGCTTGCCG CCACCATGTA 181 CCCATACGAC GTTCCAGATT ACGCTGGTAC CGACCCCGCG CAACAGCCGC CGCCCCAGCC 241 GGCCCCGCAA GGCCCCGCGC CGCCGTCCGT GTCTCCGGCC GGGACCCCCG CGGCCCCGCC 301 CGCACCCCCG GCCGGCCACC AGGTCGTGCA CGTCCGCGGG GACTCGGAGA CCGACTTGGA 361 GGCGCTCTTC AATGCCGTCA TGAACCCCAA GACGGCCAAC GTGCCTCAGA CCGTGCCCAT 421 GCGGCTTCGC AAGCTGCCCG ACTCCTTCTT CAAGCCGCCT GAGCCCAAGT CCCACTCGAG 481 ACAGGCCAGT ACTGATGCAG GTACTGCGGG AGCTCTGACT CCACAGCATG TTCGAGCTCA 541 CGCATCTCCA GCCTCCCTGC AGCTGGGTGC CGTTTCTCCT GGGACACTCA CAGCCAGTGG 601 CGTTGTCTCT GGCCCTGCCG CTGCCCCTGC AGCTCAGCAT CTCCGGCAGT CCTCCTTTGA 661 GATCCCTGAT GATGTACCAC TGCCAGCAGG CTGGGAGATG GCCAAGACAT CTTCTGGTCA 721 AAGATACTTC TTAAATCACA ACGATCAGAC NACAACATGG NAGGACCCCC GGAAGGNNNN 781 GCTTTCNNAA CTGANNGTTC CTGCGCCTGC CAGCCCAGCG GTGNCNCAGA CGCTGATGAA 841 TTCTGNCTCN GGACCTCTTC CTGATGGATG GGANCNAGCC NTGACTCANG ANGGAGAANT 901 TTACTACANA AACCNTAANA ACNNGANNAC ATNCNTGNNN NGGNNCCNNN GCTGGNNCCT 961 CNNTTTTGCC NTGANNNNAN NNNANCACTN NNANNGNNNC ANNNGNANNC NNCCCCCNCC 1021 CNTNGNNTNC CNNNANCCNN NNNNGGNNNN NNNNNN // |
Primer: GNP primer #10 (Pr0763) Region: TRF2-3'UTR+pA83, Myc tag, insert 3' Sequence file: RDB12195_A3Icb.seq ![]() |
>12195_3_GNP10_F09_18.ab1 1 TNNNNNNNNN NNNNTNATTN NNNNNNNAAC GTTTACNGGT TCTTTGGTGC CCATAGTCGA 61 GCGGCCGCGG ATCCCTAGTT CAGGTCCTCC TCGGAGATCA GCTTCTGCTC AAGCTTGGGC 121 TGCATAGATC CCAGAGCAGA TTTCTCTGGA TTGGCATCGC TCATCTTGGA GCTCATGGGC 181 GGCGGGATGA CGGCATTGGC TGCACTCCTG CGCAGGCGAC TCTGCACGAT TTCCTCGTGT 241 ATCCTGGGAT AGGCAGCCGG ATTGAAGCAG TTAAGGAAGT TGGCCCGTTC CAGCATCATG 301 GCCGCAGTAG CGGCTGCGGC GGCAGCTTGC TGTTGCCGGC TAAACTCCAG CAGATCCACT 361 CTTGTAAAGG CGGAGGGCGT GGCAGTCTGG GAGCGATTCG CCTCGAAGGG GCGGAATATG 421 CTGGCCTGGT TCTCAATCGA CTTGCCAGGC TTCTGGACAT CTCCGAAACG ATCGCTCAGG 481 ATGTTGGAGA TGGAAAAGGC CAGCGAGGGC TTGGCCGTCG ACTGCTGCTG GTGGGCGGGT 541 GGTGGCAGGC GTCCGCCCGC CGACGTATCA TCCACATCCA CATCAATGTC GTCATCCTCC 601 TCCGCCTCCT CCTCCTCGTT GGTGGTGTGC GTCTGATTGT GGAAACTCAT GTCACCGATC 661 ACGGAGTCGC TTTCCTCCTC CTTGATGGTC AGCGGCGTGG ACGANGCGGG CGTGGAGCAN 721 GATGCGGGCG ATCCNCTGCC ACTGANGCGC TGCTGCAGTG CAGCAGCATG AGCGTGGGCG 781 GNCGCAGCAG CAGCTGCAGC AGCGGCGGCT GCAGCGGCAT CGAAGGNCAT TGCCGGANGG 841 NGNAANANAC CGGCGGCCAN TTGCTGTTGG TGCAACNGCT GCAGTTGCTG CANCTGGNNN 901 ANGNGCTGCA TTTGCTGNTG CTGTTGCTGC TGCTGCTGNT GCTNGNGGTG AANANGCTGC 961 ATTTGNNNGG NAATGGGGCN GGGCNNNNNN NGNNNNNNNN ANCGATNCNN NNNGGNNNCN 1021 ANNNNNNNNN N // |
Primer: BGH_rev2 (Pr0606) Region: TRF2-3'UTR+pA83 Sequence file: RDB12195_A3Icc.seq ![]() |
>12195_12195_B0CjF_BGH_rev2_F02_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNGGN GGNNGGNNNN NNACNGATGG CTGGCAACTA GAAGGCACAG 61 TCGAGGCTGA TCANCGGGTT TNNNNNNNAT GGTGATGGTG ATGATGACCG GTATGCATAT 121 TCAGATCCTC TTCTGAGATG AGTTTTTGTT CGAAGGGCCC TCTAGACTCG AGGTACGCCA 181 CAGCGTATGA TGCGTATTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT 241 TTTTTTTTTT TTTTTTTTTT TTTTTNNNNN NNNNN // |
Primer: Yap-M_F (Pr0286) Region: insert mid Sequence file: RDB12195_A3Icd.seq ![]() |
>12195_12195_B0CjF_Yap-M_F_G02_07_ABI08.ab1 1 NNNNNNNNNN NNNNTTNNNN NNTGNNCGTT CCTGCGCCTG CCNGCCCAGC GGTGCCCCAG 61 ACGCTGATGA ATTCTGCCTC AGGACCTCTT CCTGATGGAT GGGAGCAAGC CATGACTCAG 121 GATGGAGAAG TTTACTACAT AAACCATAAG AACAAGACCA CATCCTGGCT GGACCCAAGG 181 CTGGACCCTC GTTTTGCCAT GAACCAGAGG ATCACTCAGA GTGCTCCAGT GAAGCAGCCC 241 CCACCCTTGG CTCCCCAGAG CCCACAGGGA GGCGTCCTGG GTGGAGGCAA GCTAGATAAA 301 GAAAGCTTTC TCACGTGGTT AATCGATGCC CTGGAGGATC GCTGCAGCCC ACAGTCAGCG 361 CCCAGCCCCA TTACCCTACA AATGCAGCAT CTTCACCACC AGCAACAGCA GCAGCAGCAA 421 CAGCAGCAGC AAATGCAGCA CCTCCACCAG CTGCAGCAAC TGCAGCAGTT GCACCAACAG 481 CAACTGGCCG CCGGTGTCTT CCACCATCCG GCAATGGCCT TCGATGCCGC TGCAGCCGCC 541 GCTGCTGCAG CTGCTGCTGC GGCCGCCCAC GCTCATGCTG CTGCACTGCA GCAGCGCCTC 601 AGTGGCAGTG GATCGCCCGC ATCCTGCTCC ACGCCCGCCT CGTCCACGCC GCTGACCATC 661 AAGGANGANG AAAGCGACTC CGTGATCGGT GACNTGAGTT TCCACAATCN NACGCACACC 721 ACCNACGANG ANGANGANGC GGANGANGAN GANGACNTTG ATGTGGNNGT GNATGANACG 781 TCNGNGGGCN NANGCCTGNN NNNACCCGNC CNNNAGCAGC AGTCNANNGN NNAGCCNNNN 841 NTGNNNTTTT NNNTCTNNNN CNTNNNNANC GANCGTTTCN NANNNGNNNN NNNNNN // |
Primer: SV40pro_ori_F (Pr0731) Region: SV40pro_ori, NeoR Sequence file: RDB12195_A3Ice.seq ![]() |
>12195_12195_B0CjF_SV40pro_ori_F_H02_08_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN CCNCTGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT 61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC 121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG 181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG 241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA 301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT 361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA 421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC 481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC 541 CTGCCNATTC GACCACCAAG CGAAACATCG CATCNAGCGA NCACGTACTC GGANGGAANN 601 NGGNCNTGNC NATCAGGANG ANCNGNANNA NNANCATCNN GGGCTCNCGC NNGCCNAACN 661 GNNCGCCNNG NNCNANGNNC NCATGNCNNA NNNNNANNAN NNCNNCNNGN NCNNNGGNNA 721 NGNNNGNNNN NCNANNNNNN NGNNNNNAAN NGNNNNNTTT NNNNNANTCN TCNANNGNNN 781 NNNNTGGNNN NNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. [link to RRC of NBRP] |
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