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pcDNA3.1-pA83-dnYap (#RDB12195)

Expression clone of mouse Yap1, dominant negative

Clone info. Expression clone of mouse Yap1, dominant negative. cDNA fragment 210 to 1,056 of NM_009534.3 (CDS, 207..1625) is cloned. S112A mutation, deletion of activation domain, Engrailed repression domain are constructed.
Comment See Fig. 5A of Nishioka et al., Dev. Cell 16: 398-410 (2009). HA-tag is fused before Yap1, and Myc-tag is fused after Engrailed repression domain. The expression vector has a stretch of poly(A). Known differences: G to C substitution at 212 nt (Glu to Asp at 2nd aa). C to A substitution at 477 nt (Arg at 91st aa, synonymous) and XhoI site insertion at the position.
Vector backbone pcDNA3.1-pA83 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Mouse Yap1
Depositor|Developer Sasaki, Hiroshi |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12195 pcDNA3.1-pA83-dnYap DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-pA83-dnYap was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12195).

Reference section:

Nishioka, N., Inoue, K., Adachi, K., Kiyonari, H., Ota, M., Ralston, A., Yabuta, N., Hirahara, S., Stephenson, R.O., Ogonuki, N., Makita, R., Kurihara, H., Morin-Kensicki, E.M., Nojima, H., Rossant, J., Nakao, K., Niwa, H., Sasaki, H., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12195_A3Icp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: HA tag, insert 5'
Sequence file: RDB12195_A3Ica.seq check
>12195_3_CMV_F_F11_17.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGGGCGG TAGGCGTGTA CGGTGGGAGG
   61 TCTATATAAG CAGAGCTCTC TGGNNAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA
  121 TTAATACGAC TCACTATAGG GAGACCCAAG CTGGCTAGTT AAGCTTGCCG CCACCATGTA
  181 CCCATACGAC GTTCCAGATT ACGCTGGTAC CGACCCCGCG CAACAGCCGC CGCCCCAGCC
  241 GGCCCCGCAA GGCCCCGCGC CGCCGTCCGT GTCTCCGGCC GGGACCCCCG CGGCCCCGCC
  301 CGCACCCCCG GCCGGCCACC AGGTCGTGCA CGTCCGCGGG GACTCGGAGA CCGACTTGGA
  361 GGCGCTCTTC AATGCCGTCA TGAACCCCAA GACGGCCAAC GTGCCTCAGA CCGTGCCCAT
  421 GCGGCTTCGC AAGCTGCCCG ACTCCTTCTT CAAGCCGCCT GAGCCCAAGT CCCACTCGAG
  481 ACAGGCCAGT ACTGATGCAG GTACTGCGGG AGCTCTGACT CCACAGCATG TTCGAGCTCA
  541 CGCATCTCCA GCCTCCCTGC AGCTGGGTGC CGTTTCTCCT GGGACACTCA CAGCCAGTGG
  601 CGTTGTCTCT GGCCCTGCCG CTGCCCCTGC AGCTCAGCAT CTCCGGCAGT CCTCCTTTGA
  661 GATCCCTGAT GATGTACCAC TGCCAGCAGG CTGGGAGATG GCCAAGACAT CTTCTGGTCA
  721 AAGATACTTC TTAAATCACA ACGATCAGAC NACAACATGG NAGGACCCCC GGAAGGNNNN
  781 GCTTTCNNAA CTGANNGTTC CTGCGCCTGC CAGCCCAGCG GTGNCNCAGA CGCTGATGAA
  841 TTCTGNCTCN GGACCTCTTC CTGATGGATG GGANCNAGCC NTGACTCANG ANGGAGAANT
  901 TTACTACANA AACCNTAANA ACNNGANNAC ATNCNTGNNN NGGNNCCNNN GCTGGNNCCT
  961 CNNTTTTGCC NTGANNNNAN NNNANCACTN NNANNGNNNC ANNNGNANNC NNCCCCCNCC
1021 CNTNGNNTNC CNNNANCCNN NNNNGGNNNN NNNNNN
//
Primer: GNP primer #10 (Pr0763)
Region: TRF2-3'UTR+pA83, Myc tag, insert 3'
Sequence file: RDB12195_A3Icb.seq check
>12195_3_GNP10_F09_18.ab1
    1 TNNNNNNNNN NNNNTNATTN NNNNNNNAAC GTTTACNGGT TCTTTGGTGC CCATAGTCGA
   61 GCGGCCGCGG ATCCCTAGTT CAGGTCCTCC TCGGAGATCA GCTTCTGCTC AAGCTTGGGC
  121 TGCATAGATC CCAGAGCAGA TTTCTCTGGA TTGGCATCGC TCATCTTGGA GCTCATGGGC
  181 GGCGGGATGA CGGCATTGGC TGCACTCCTG CGCAGGCGAC TCTGCACGAT TTCCTCGTGT
  241 ATCCTGGGAT AGGCAGCCGG ATTGAAGCAG TTAAGGAAGT TGGCCCGTTC CAGCATCATG
  301 GCCGCAGTAG CGGCTGCGGC GGCAGCTTGC TGTTGCCGGC TAAACTCCAG CAGATCCACT
  361 CTTGTAAAGG CGGAGGGCGT GGCAGTCTGG GAGCGATTCG CCTCGAAGGG GCGGAATATG
  421 CTGGCCTGGT TCTCAATCGA CTTGCCAGGC TTCTGGACAT CTCCGAAACG ATCGCTCAGG
  481 ATGTTGGAGA TGGAAAAGGC CAGCGAGGGC TTGGCCGTCG ACTGCTGCTG GTGGGCGGGT
  541 GGTGGCAGGC GTCCGCCCGC CGACGTATCA TCCACATCCA CATCAATGTC GTCATCCTCC
  601 TCCGCCTCCT CCTCCTCGTT GGTGGTGTGC GTCTGATTGT GGAAACTCAT GTCACCGATC
  661 ACGGAGTCGC TTTCCTCCTC CTTGATGGTC AGCGGCGTGG ACGANGCGGG CGTGGAGCAN
  721 GATGCGGGCG ATCCNCTGCC ACTGANGCGC TGCTGCAGTG CAGCAGCATG AGCGTGGGCG
  781 GNCGCAGCAG CAGCTGCAGC AGCGGCGGCT GCAGCGGCAT CGAAGGNCAT TGCCGGANGG
  841 NGNAANANAC CGGCGGCCAN TTGCTGTTGG TGCAACNGCT GCAGTTGCTG CANCTGGNNN
  901 ANGNGCTGCA TTTGCTGNTG CTGTTGCTGC TGCTGCTGNT GCTNGNGGTG AANANGCTGC
  961 ATTTGNNNGG NAATGGGGCN GGGCNNNNNN NGNNNNNNNN ANCGATNCNN NNNGGNNNCN
1021 ANNNNNNNNN N
//
Primer: BGH_rev2 (Pr0606)
Region: TRF2-3'UTR+pA83
Sequence file: RDB12195_A3Icc.seq check
>12195_12195_B0CjF_BGH_rev2_F02_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNGGN GGNNGGNNNN NNACNGATGG CTGGCAACTA GAAGGCACAG
   61 TCGAGGCTGA TCANCGGGTT TNNNNNNNAT GGTGATGGTG ATGATGACCG GTATGCATAT
  121 TCAGATCCTC TTCTGAGATG AGTTTTTGTT CGAAGGGCCC TCTAGACTCG AGGTACGCCA
  181 CAGCGTATGA TGCGTATTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
241 TTTTTTTTTT TTTTTTTTTT TTTTTNNNNN NNNNN
//
Primer: Yap-M_F (Pr0286)
Region: insert mid
Sequence file: RDB12195_A3Icd.seq check
>12195_12195_B0CjF_Yap-M_F_G02_07_ABI08.ab1
    1 NNNNNNNNNN NNNNTTNNNN NNTGNNCGTT CCTGCGCCTG CCNGCCCAGC GGTGCCCCAG
   61 ACGCTGATGA ATTCTGCCTC AGGACCTCTT CCTGATGGAT GGGAGCAAGC CATGACTCAG
  121 GATGGAGAAG TTTACTACAT AAACCATAAG AACAAGACCA CATCCTGGCT GGACCCAAGG
  181 CTGGACCCTC GTTTTGCCAT GAACCAGAGG ATCACTCAGA GTGCTCCAGT GAAGCAGCCC
  241 CCACCCTTGG CTCCCCAGAG CCCACAGGGA GGCGTCCTGG GTGGAGGCAA GCTAGATAAA
  301 GAAAGCTTTC TCACGTGGTT AATCGATGCC CTGGAGGATC GCTGCAGCCC ACAGTCAGCG
  361 CCCAGCCCCA TTACCCTACA AATGCAGCAT CTTCACCACC AGCAACAGCA GCAGCAGCAA
  421 CAGCAGCAGC AAATGCAGCA CCTCCACCAG CTGCAGCAAC TGCAGCAGTT GCACCAACAG
  481 CAACTGGCCG CCGGTGTCTT CCACCATCCG GCAATGGCCT TCGATGCCGC TGCAGCCGCC
  541 GCTGCTGCAG CTGCTGCTGC GGCCGCCCAC GCTCATGCTG CTGCACTGCA GCAGCGCCTC
  601 AGTGGCAGTG GATCGCCCGC ATCCTGCTCC ACGCCCGCCT CGTCCACGCC GCTGACCATC
  661 AAGGANGANG AAAGCGACTC CGTGATCGGT GACNTGAGTT TCCACAATCN NACGCACACC
  721 ACCNACGANG ANGANGANGC GGANGANGAN GANGACNTTG ATGTGGNNGT GNATGANACG
  781 TCNGNGGGCN NANGCCTGNN NNNACCCGNC CNNNAGCAGC AGTCNANNGN NNAGCCNNNN
841 NTGNNNTTTT NNNTCTNNNN CNTNNNNANC GANCGTTTCN NANNNGNNNN NNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40pro_ori, NeoR
Sequence file: RDB12195_A3Ice.seq check
>12195_12195_B0CjF_SV40pro_ori_F_H02_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN CCNCTGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT
   61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
  121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG
  181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG
  241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA
  301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT
  361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA
  421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC
  481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC
  541 CTGCCNATTC GACCACCAAG CGAAACATCG CATCNAGCGA NCACGTACTC GGANGGAANN
  601 NGGNCNTGNC NATCAGGANG ANCNGNANNA NNANCATCNN GGGCTCNCGC NNGCCNAACN
  661 GNNCGCCNNG NNCNANGNNC NCATGNCNNA NNNNNANNAN NNCNNCNNGN NCNNNGGNNA
  721 NGNNNGNNNN NCNANNNNNN NGNNNNNAAN NGNNNNNTTT NNNNNANTCN TCNANNGNNN
781 NNNNTGGNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. [link to RRC of NBRP]