Resource data sheet
Prev.

pMYs-HA-Yap1 DWW-IRES-EGFP

Retroviral vector of mouse Yap1, deletion of WW domain

Catalog number RDB12179
Resource name pMYs-HA-Yap1 DWW-IRES-EGFP
Clone info. Retroviral vector of mouse Yap1, deletion of WW domain. cDNA fragments 210 to 681 nt and 947 to 1,625 nt of NM_009534.3 (CDS, 207..1625) are cloned.
Transfection can be confirmed by GFP. See Fig. 3A of Ota and Sasaki, Development 135: 4059-4069 (2008). Known differences: G to C substitution at 212 nt (Glu to Asp at 2nd aa). C to A substitution at 477 nt (Arg at 91st aa, synonymous) and XhoI site insertion at the position.
Vector backbone pMYs-IRES-EGFP (Plasmid)
Size of vector backbone 7.1 kb
Selectable markers Amp^r
Gene/insert name Mouse Yap1 cDNA
Depositor Sasaki, Hiroshi |
 
Other clones in our bank mouse Yap1 (NCBI Gene 22601) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Additional terms and conditions for distribution Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
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Schedule A (GFP-A) [Link]
Remarks Remember that you will be working with samples containing infections virus.
Please use Schedule A (GFP-A) additionally.
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RDB12179 pMYs-HA-Yap1 DWW-IRES-EGFP DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12179_A6Ajp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: 5'Retro (pFB-5') RDB12179_A6Aja.seq
>12179_12179_A6Aj_5'Retro(pFB-5')_G10_19.ab1
    1 TTCCGCTTGA GAGGTTTCTC GGACTAGCTA GTTATTAAGG ATCCCAGTGT GGTGGTACGG
   61 GAATTCCTGC AGGCCATGTA CCCATACGAC GTTCCAGATT ACGCTGGTAC CGACCCCGCG
  121 CAACAGCCGC CGCCCCAGCC GGCCCCGCAA GGCCCCGCGC CGCCGTCCGT GTCTCCGGCC
  181 GGGACCCCCG CGGCCCCGCC CGCACCCCCG GCCGGCCACC AGGTCGTGCA CGTCCGCGGG
  241 GACTCGGAGA CCGACTTGGA GGCGCTCTTC AATGCCGTCA TGAACCCCAA GACGGCCAAC
  301 GTGCCTCAGA CCGTGCCCAT GCGGCTTCGC AAGCTGCCCG ACTCCTTCTT CAAGCCGCCT
  361 GAGCCCAAGT CCCACTCGAG ACAGGCCAGT ACTGATGCAG GTACTGCGGG AGCTCTGACT
  421 CCACAGCATG TTCGAGCTCA CTCCTCTCCA GCCTCCCTGC AGCTGGGTGC CGTTTCTCCT
  481 GGGACACTCA CAGCCAGTGG CGTTGTCTCT GGCCCTGCCG CTGCCCCTGC AGCTCAGCAT
  541 CTCCGGCAGT CCTCCTTTGA GATCCCTGAT GATGTACCAC TGCTGGACCC TCGTTTTGCC
  601 ATGAACCAGA GGATCACTCA GAGTGCTCCA GTGAAGCAGC CCCCACCCTT GGCTCCCCAG
  661 AGCCCACAGG GAGGCGTCCT GGGTGGAGGC AGTTCCAACC AGCAGCAGCA AATACAGCTG
  721 CAGCAGTTAC AGATGGAGAA GGAGAGACTG CGGTTGAAAC AACAGGAATT ATTTCGGCAG
  781 GAATTAGCTC TGCGCAGCCA GTTGCCTACA CTGGAGCAGG ATGGAGGGAC TCCGAATGCA
  841 GTGTCTTCTC CTGGGATGTC TCAGGAATTG AGAACAATGA CAACCAATAG TTCCGATCCC
  901 TTTCTTAACA GTGGCACCTA TCACTCTCGA GATGAGAGCA CAGACAGCGG GCCTCAGCAT
  961 GAGCAGCTAC AGCATCCCTC GGACCCCAGA CGACTTTCTC ACAGTGTGGA TGAATGGATA
 1021 CAGGAGACAC CATCAGCCAA GCACCCTTGC GTCACAGCAG AGCCGCTTCC CCGGACTACT
 1081 GAGCCTCCCT GGGACAATGT GGACCTTGCC ACTGAGGAAT GCCATGACAT AGAGGGGGGG
1141 AGGGAGCTGT GATGC
//
Primer: IRES-R RDB12179_A6Ajb.seq
>12179_12179_A6Aj_IRES-R_G08_20.ab1
    1 GCATAGGCGG GCTTCGGGCC AGTACGTTAG GGGGGGGGGA GGGAGAGGGG GGGGGGGGGC
   61 GGAATTTACG TAGCGGCCGC GGCGCGCCGG CCCTCGAGGC TCTATAACCA CGTGAGAAAG
  121 CTTTCTTTAT CTAGCTTGGT GGCAGCCAAC ACAGACTCCA CGTCCAAGAT TTCGGAACTC
  181 AGCGCTTCCT GCAGACTGGG CATCAGCTCC TCCCCTTCTA TGTTCATTGC ATCTCCTTCC
  241 AGTGTGCCAA GGTCCACATT TGTCCCAGGG AGGGCTTCCA GGTAGTCGGG GAAGCGGCTC
  301 TGCTGTGACG GCAGGGTGCT TTGGCTGATG GTGTCTCCTG TATCCATTTC ATCCACACTG
  361 TTGAGGAAGT CGTCTGGGGT CCGAGGGATG CTGTAGCTGC TCATGCTGAG GCCGCTGTCT
  421 GTGCTCTCAT CTCGAGAGTG ATAGGTGCCA CTGTTAAGAA AGGGATCGGA ACTATTGGTT
  481 GTCATTGTTC TCAATTCCTG AGACATCCCA GGAGAAGACA CTGCATTCGG AGTCCCTCCA
  541 TCCTGCTCCA GTGTAGGCAA CTGGCTGCGC AGAGCTAATT CCTGCCGAAA TAATTCCTGT
  601 TGTTTCAACC GCAGTCTCTC CTTCTCCATC TGTAACTGCT GCACCTGTAT TTGCTGCTGC
  661 TGGTTGGAAC TGCCTCCACC CAGGACGCCT CCCTGTGGGC TCTGGGGAGC CAAGGGTGGG
  721 GGCTGCTTTC ACTGGAGCAC TCTGAGTGAT CCTCTGGTTC ATGGCAAAAA CGAGGGTCCA
  781 GCAGTGGTAC ATCATCAGGG GATCTCAAAG GAAGGACTGC CGGAGATGCT GAGCTGCAAG
  841 GGGCAGCGGC AAGGGCCCAG AAGACAAACG CCACTGGCTG TGAGTGTCCC AAGAAAAAAC
  901 GGCACCCCAG CTGCAGGGAA GGCTGGGAAA GGAAGTGGAG CTTCGAACAT GCTTGTGGAA
  961 GTCAAAGCCT CCCGCAATAC CTTGCATCCA TTACTTGGCC CTGTCCTCCG ATGGGGAATT
 1021 TTGGGCTTCA GGCGGCTTGA AAAAAAGGAA TCCGGGCAGC TTGTCAACAC CGCCATAGGG
1081 CACACGGGTC TTAAAGGCAC ACAT
//
Primer: IRES-F RDB12179_A6Ajc.seq
>12179_12179_A6Aj_IRES-F_H08_23.ab1
    1 GAATTCCTTT TATGTGTTTA GTCGAGGTTA AAAAAACGTC TAGGCCCCCC GAACCACGGG
   61 GACGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGG TGAGCAAGGG
  121 CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG
  181 CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT
  241 GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT
  301 GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT
  361 CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG
  421 CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA
  481 GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA
  541 CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA
  601 CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA
  661 GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA
  721 GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT
  781 GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTAAAGCG GGCCGGCCGC
  841 CAGCACAGTG GTCGACCGGG CCGCCAGCAC AGTGGTCGAT CGAGGGGGGG CCCGCGATTA
  901 GTCCAATTTG TTAAAAGACA GGATATCAGT GGTCCAGGCT CTAGTTTTGA CTCAACAATA
  961 TCACCAGCTG AAGCCCTATA GAGTACGAGC CCATAGATAG AATAAAAGAT TTTTATTTAA
 1021 GTCTCCAAGA AAAAAGGGGG GGAATGAAAG AACCCCCACC TGTTAGGGTT GGGCAAGCCT
 1081 AGCCTAAGTT AACGGCCATT TGGCCAAAGG CCATGAAAAT ACATACCTGA GAAATTAGAG
1141 AAAGCT
//
Primer: Amp_R RDB12179_A6Ajd.seq
>12179_12179_A6Aj_Amp_R_A09_03.ab1
    1 TTTGTTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGCATAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT
  181 GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAAAAGTT CAGATCAAGG TCAGGAACAG
  241 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT
  301 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA
  361 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC
  421 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG
  481 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC
  541 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ATTGACTGAG
  601 TCGCCCGGGT ACCCGTATTC CCAATAAAGC CTCTTGCTGT TTGCATCCGA ATCGTGGACT
  661 CGCTGATCCT TGGGAGGGTC TCCTCAGATT GATTGACTGC CCACCTCGGG GGTCTTTCAT
  721 TTGGAGGTTC CACCGAGATT TGGAGACCCC TGCCCAGGGA CCACCGACCC CCCCGCCGGG
  781 AGGTAAGCTG GCCAGCGGTC GTTTCGTGTC TGTCTCTGTC TTTGTGCGTG TTTGTGCCGG
  841 CATCTAATGT TTGCGCCTGC GTCTGTACTA GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC
  901 CCGTGGTGGA AACTGACGAG TTCGGAACAC CCGGGCCGCA ACCCTGGGAG ACGTCCCAGG
  961 GACTTCGGGG GCCGTTTTTG TGGCCGACCT GAGTCCAAAA TCCGATCGTT TTGGACTCTT
 1021 TGGTGCACCC CCCTATAGAA GGGATATGTG GCTCCTGTTA GAGACGAGAC TAACAGTCCG
1081 GCTCGGTCTG ACTTTTGCTT CGGTTGGGAC GAAG
//
Primer: pMFG_1 RDB12179_A6Aje.seq
>12179_12179_A6Aj_pMFG-1_B09_06.ab1
    1 GGAAAGGGAA AGGAGGGGCT GTTAGTAACT CTTGTCATGT CAGGGTCAGG GAAGTTTACA
   61 AGGGGCGGGG GTGCCCCATA TAAGATCTCA TATGGCCATA TGGGGGCGCC TAGAGAAGGA
  121 GTGAGGGCTG GATAAAGGGA GGATCGAGGC GGGGTCGAAC GAGGAGGTTC AAGGGGGAGA
  181 GACGGGGCGG ATGGAGGAAG AGGAGGCGGA GGCTTAGGGT GTACAAAGGG CTTGACCCAG
  241 GGAGGGGGGT CAAAAGCCAA GGCTTCCCAG GTCACGATGT AGGGGACCTG GTCTGGGTGT
  301 CCATGCGGGC CAGGTGAAAA GACCTTGATC TTAACCTGGG TGATGAGGTC TCGGTTAAAG
  361 GTGCCGTCTC GCGGCCATCC GACGTTAAAG GTTGGCCATT CTGCAGAGCA GAAGGTAACC
  421 CAACGTCTCT TCTTGACATC TACCGACTGG TTGTGAGCGA TCCGCTCGAC ATCTTTCCAG
  481 TGACCTAAGG TCAAACTTAA GGGAGTGGTA ACAGTCTGGC CCTAATTTTC AGACAAATAC
  541 AGAAACACAG TCAGACAGAG ACAACACAGA ACGATGCTGC AGCAGACAAG ACGCGCGGCG
  601 CGGCTTCGGT CCCAAACCGA AAGCAAAAAT TCAGACGGAG GCGGGAACTG TTTTAGGTTC
  661 TCGTCTCCTA CCAGAACCAC ATATCCCTCC TATTAGGGGG GTGCACCAAA GAGTCCAAAA
  721 CGATCGGGAT TTTTGGACTC AGGTCGGGCC ACAAAAACGG CCCCCGAAGT CCCTGGGACG
  781 TCTCCCAGGG TTGCGGCCGG GTGTTCCGAA CTCGTCAGTT CCACCACGGG TCCGCCAGAT
  841 ACAGAGCTAG TTAGCTAACT AGTACAGACG CAGGCGCAAA CATTAGATGC CGGCACAAAC
  901 ACGCACAAAG ACAGAGACAG ACACGAAACG ACCGCTGGGC CAGCTTACCC TCCCGGCGGG
  961 GGGGGTCGGT GGTCCCTGGG CAGGGGGTCT CCAAATCTCG GGTGGAACCT CCGAATGAAA
 1021 GAACCCCCGA GGTGGGCAAG TCAATCAATC TGAGGAGACC CCTCCCAAGA TCAAGCCGAA
 1081 GTCCACGATT TCGGATGCAT AGCAAGCAGC CTTTTATGGG ATTACCGGTT ACCCCGGGGG
1141 CGAACT
//
Primer: EGFP-C RDB12179_A6Ajf.seq
>12179_12179_A6Aj_EGFP_C_D02_11.ab1
    1 CAAGTGTTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGGCCGCCA GCACAGTGGT
   61 CGACCGGGCC GCCAGCACAG TGGTCGATCG AGGGGGGGCC CGCGATTAGT CCAATTTGTT
  121 AAAGACAGGA TATCAGTGGT CCAGGCTCTA GTTTTGACTC AACAATATCA CCAGCTGAAG
  181 CCTATAGAGT ACGAGCCATA GATAGAATAA AAGATTTTAT TTAGTCTCCA GAAAAAGGGG
  241 GGAATGAAAG ACCCCACCTG TAGGTTTGGC AAGCTAGCTT AAGTAACGCC ATTTTGCAAG
  301 GCATGGAAAA TACATAACTG AGAATAGAGA AGTTCAGATC AAGGTTAGGA ACAGAGAGAC
  361 AGCAGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCAGGGCC
  421 AAGAACAGTT GGAACAGCAG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG
  481 CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCCGCCCTC AGCAGTTTCT
  541 AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT
  601 TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA
  661 ATAAAAGAGC CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATAGACT GCGTCGCCCG
  721 GGTACCCGTG TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT CTCGCTGTTC
  781 CTTGGGAGGG TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT CACATGCAGC
  841 ATGTATCAAA ATTAATTTGG TTTTTTTTTC TTAAGTATTT ACATTAAATG GCCCATAGTT
  901 GCATTAATGA ATCGGCCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG
  961 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC
 1021 CACTCAAAGG CGGTATACGT TATCCACAGA TCAGGGATAC GCAGAAAGAA CATGTGAGCA
1081 AGGCAGCAAG TAAGACCGTA AAAAAGGTCC G
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

2019.04.09

GNP_filter3_RDBDEP_html_190327.pl