Resource data sheet
Prev.

pMYs-Tead2FL-IRES-EGFP

Retroviral vector of mouse Tead2

Catalog number RDB12173
Resource name pMYs-Tead2FL-IRES-EGFP
Clone info. Retroviral vector of mouse Tead2 full length (FL). cDNA fragment 87 to 1,424 nt of NM_011565.2 (CDS, 87..1424) is cloned.
Transfection can be confirmed by GFP. See Fig. 2B of Ota and Sasaki, Development 135: 4059-4069 (2008). Known differences: G to A substitution at 453 nt (Val to Ile at 123rd aa).
Vector backbone pMYs-IRES-EGFP (Plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r
Gene/insert name Mouse Tead2 cDNA
Depositor Sasaki, Hiroshi |
 
Other clones in our bank mouse Tead2 (NCBI Gene 21677) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Additional terms and conditions for distribution Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks Remember that you will be working with samples containing infections virus.
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12173 pMYs-Tead2FL-IRES-EGFP DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12173_A6Ajp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: 5'Retro(pFB-5') RDB12173_A6Aja.seq
>12173_12173_A6Aj_5'Retro(pFB-5')_A07_01.ab1
    1 TTCAGCTGGA GGATCTATCG CGGACTGCTA GTTATTAAGG ATCCCAGTGT GGTGGTACGG
   61 GAATTCGAGG CCCAGATGGG GGATCCCCGG ACTGGGGCCC CCCTGGATGA TGGCGGGGGC
  121 TGGACAGGTA GCGAGGAAGG CAGCGAAGAG GGCACCGGAG GCAGCGAGGG CGTTGGAGGT
  181 GATGGCAGCC CTGATGCAGA AGGTGTGTGG AGCCCCGACA TTGAGCAGAG TTTTCAGGAG
  241 GCCCTGGCCA TCTACCCTCC CTGTGGCCGT CGCAAGATCA TCCTGTCAGA TGAGGGCAAG
  301 ATGTATGGCC GGAACGAACT CATTGCCCGT TACATCAAGC TGAGGACAGG GAAGACGAGA
  361 ACGCGAAAGC AGGTCTCCAG CCATATTCAG GTTTTGGCTC GAAGGAAATC GAGAGAAATT
  421 CAGTCCAAGC TGAAGGACCA AATCTCCAAG GACAAGGCCT TCCAGACGAT GGCCACCATG
  481 TCTTCGGCAC AGCTCATCTC CGCCCCTTCC CTCCAGGCCA AGCTGGGCCC TTCTGGCCCT
  541 CAGGCCACTG AGCTTTTCCA GTTCTGGTCA GGGAGCTCTG GGCCACCATG GAATGTTCCA
  601 GACGTGAAGC CCTTCTCACA GGCACCGTTC TCCGTGTCAC TGACGCCCCC AGCCTCTGAC
  661 CTACCAGGGT ACGAGCCGCC CCCAGCCCTC TCACCCCTGC CCCCACCCGC TCCGTCTCCC
  721 CCAGCCTGGC AGGCTCGGGC CCTGGGCACT GCCCGCCTGC AGCTGATAGA GTTCTCAGCG
  781 TTTGTGGAAC CGCCAGACGC AGTTGACTCG TTCCAGAGGC ATCTGTTTGT CCACATCAGT
  841 CAGCAGTGTC CCAGCCCTGG AGCACCACCC CTAGAGAGTG TGGACGTGCG GCAGATCTAC
  901 GACAAATTCC CTGAGAAGAA GGGCGGCCTC CGCGAGCTGT ATGACCGAGG GCCACCACAT
  961 GCCTTTCTTC CTCGTCAAGT TCTGGGCGGA CCTGAACTGG GGCCCCAGTG CCGAGAGGCA
 1021 GGAGCAGCGG GAGGTGGCCG TGCTTCTATG GAGGTGAGCA GCAGTAATGA GAGCAGGGAG
 1081 CTCATGACAC TTCACTGCTC TTCAAGGTCT GCTTCTTGCC AGCCAGTGGT AAGAGAAAGT
//
Primer: IRES-R RDB12173_A6Ajb.seq
>12173_12173_A6Aj_IRES-R_H11_23.ab1
    1 GCCATCGGCG GGCTTCGGCA GTACGTTAGG GGGGGGGGAG GGAGAGGGGG GGGGGGGGCG
   61 GAATTTACGT AGCGGCCGCG GCGCGCCGGC CCTCGAGATC AGTCCCTGAC CAGGCGGTAG
  121 ATGTGGTACT GGGCTCCTCG TTCACTGGTG GAGACTTCAA AGACGTAGGC AGTACACAGC
  181 AGCAGTTCCT GAGTGTCCCT GTTTGTCACA ACCTGGAGGA TGGTGAAGTT CTCCAGGACA
  241 CTGTTCATCA TGTAGCGTTC AGGCAGCTGA CGGAGCTTGT GCAGGAAATT AACCAGGTAC
  301 TCACACATGG GAGAGCGCAG CAGACGGTAC ACAAAGCGCC CGTCCTCCAG CTGGGCCCGT
  361 TCCGTCTCCA CCTTCTCTAC CACTTGCTTG CCAAAGGAGC AGACCTTGGA GGAGCAGGTG
  421 AGTGTCATGA GCTCCCGGCT CTCATACTGG CTGCTCACTC CATAGAAGCC ACCGCCACCT
  481 CCGCTGCTCC CTGCCTCCTC GGCACTGGGG CCCCAGTTCA GGTCCGCCCA GAACTTGACG
  541 AGGAAGAAGG CATGTGGTGG CCCTCGGTCA TACAGCTCGC GGAGGCCGCC CTTCTTCTCA
  601 GGGAATTTGT CGTAGATCTG CCGCACGTCC ACACTCTCTA GGGGTGGTGC TCCAGGGCTG
  661 GGACACTGCT GACTGATGTG GACAAACAGA TGCCTCTGGA ACGAGTCAAC TGCGTCTGGC
  721 GGTTCCACAA ACGCTGAGAA CTCTATCAGC TGCAGGCGGG CAGTGCCCAG GGCCCGAGCC
  781 TGCCAGGCTG GGGGAGACGG AGCGGGTGGG GGCAGGGGTG AGAGGGCTGG GGGCGGCTCG
  841 TACCCTGGTA GGTCAGAGGC TGGGGGCGTC AGTGACACGG AGAACGGTGC CTGTGAGAAG
  901 GGCTTCACGT CTGGGAACAT TCCATGGTGG GCCCAGAGCT CCCTGACCAG AACTGGGAAA
  961 AGCTCAGTGG CCCTGAGGGC CCAGAAGGGC CCAGCTTGGC CTGAGGGAAG GGCGGGAGAT
 1021 GAGCTGTGCC GAAGACATGG TGGGCATCGT CTGAGCTTGT CCTGGAGATT GTTCTCAGCC
 1081 TGACTGAATT TCTCTCGAAT TGCATCGAGG CCGAACTGAT ATGTTGAGAA CCTGCTCTTC
1141 G
//
Primer: IRES_F RDB12173_A6Ajc.seq
>12173_12173_A6Aj_IRES-F_A08_02.ab1
    1 GCAGTCGTCT GTGTTTAGTC GAGGTTAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG
   61 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGGTGAG CAAGGGCGAG
  121 GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC
  181 AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG
  241 TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC
  301 TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG
  361 TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC
  421 TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG
  481 AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC
  541 AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC
  601 AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC
  661 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC
  721 GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC
  781 GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT AAAGCGGCCG GGCCGCCAGC
  841 ACAGTGGTCG ACCGGGCCGC CAGCACAGTG GTCGATCGAG GGGGGGCCCG CGATTAGTCC
  901 AAATTTGTTA AAGAACAGGA TATCAGTGGT CAGCTCTAGT TTTTGACTCA ACAATATCAC
  961 CAGCTGAGGC TATAGAGTAC GAGCAGATGG ATTACGATTT ATTTAGTCTC AGAAAACGGG
 1021 GAATGGAAGA ACCCACTTGT AGTGGCAAGC TAGCCTTAGT AGCATGCACG CATGGAATAC
1081 CTAACTTGAA G
//
Primer: Amp_R RDB12173_A6Ajd.seq
>12173_12173_A6Aj_Amp_R_C07_07.ab1
    1 TTCCGTTGTT TATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
   61 TTTGAATGTA TTTAGAAAAT TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG
  121 CCACCTGCAT AATGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT
  181 TTTGCAAGGC ATGGAAAAAT ACATAACTGA GAATAGAAAA GTTCAGATCA AGGTCAGGAA
  241 CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG
  301 GCTCAGGGCC AAGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA
  361 GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCAGCCCTC
  421 AGCAGTTTCT AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT
  481 GTGCCTTATT TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC
  541 CCCGAGCTCA ATAAAAGAGC CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATTGACT
  601 GAGTCGCCCG GGTACCCGTA TTCCCAATAA AGCCTCTTGC TGTTTGCATC CGAATCGTGG
  661 ACTCGCTGAT CCTTGGGAGG GTCTCCTCAG ATTGATTGAC TGCCCACCTC GGGGGTCTTT
  721 CATTTGGAGG TTCCACCGAG ATTTGGAGAC CCCTGCCCAG GGACCACCGA CCCCCCCGCC
  781 GGGAGGTAAG CTGGCCAGCG GTCGTTTCGT GTCTGTCTCT GTCTTTGTGC GTGTTTGTGC
  841 CGGCATCTAA TGTTTGCGCC TGCGTCTGTA CTAGTTAGCT AACTAGCTCT GTATCTGGCG
  901 GACCCGTGGT GGAACTGACG AGTTCGGAAC ACCCGGGCCG CAACCCTGGG AGACGTCCCA
  961 GGGACTTCGG GGGGCCGTTT TTGTGGCCCG ACCTGAGTCC AAAAATCCCG ATCGTTTTGG
 1021 ACTCTTTTGG TGCACCCCCC CTATAGAGGA TATGTGGTTC TGGTAGGAGA CGAGACTAAA
 1081 CAGTTTCCCG CCTCCGTCTG ATTTTGCTTC GAATGGACGA GACTGGATGC GTCGTAGCTT
1141 GCTGTGTGCA G
//
Primer: pMFG-1 RDB12173_A6Aje.seq
>12173_12173_A6Aj_pMFG-1_D07_10.ab1
    1 GCTCAGGGGG GGAGGAGGGG CTGTTAGTAC TCTTGTCATG TCAGGGTCAG GGAAGTTTAC
   61 AAGGGGCGGG GGTGCCCCAT ATAAGATCTC ATATGGCCAT ATGGGGGCGC CTAGAGAAGG
  121 AGTGAGGGCT GGATAAAGGG AGGATCGAGG CGGGGTCGAA CGAGGAGGTT CAAGGGGGAG
  181 AGACGGGGCG GATGGAGGAA GAGGAGGCGG AGGCTTAGGG TGTACAAAGG GCTTGACCCA
  241 GGGAGGGGGG TCAAAAGCCA AGGCTTCCCA GGTCACGATG TAGGGGACCT GGTCTGGGTG
  301 TCCATGCGGG CCAGGTGAAA AGACCTTGAT CTTAACCTGG GTGATGAGGT CTCGGTTAAA
  361 GGTGCCGTCT CGCGGCCATC CGACGTTAAA GGTTGGCCAT TCTGCAGAGC AGAAGGTAAC
  421 CCAACGTCTC TTCTTGACAT CTACCGACTG GTTGTGAGCG ATCCGCTCGA CATCTTTCCA
  481 GTGACCTAAG GTCAAACTTA AGGGAGTGGT AACAGTCTGG CCCTAATTTT CAGACAAATA
  541 CAGAAACACA GTCAGACAGA GACAACACAG AACGATGCTG CAGCAGACAA GACGCGCGGC
  601 GCGGCTTCGG TCCCAAACCG AAAGCAAAAA TTCAGACGGA GGCGGGAACT GTTTTAGGTT
  661 CTCGTCTCCT ACCAGAACCA CATATCCCTC CTATTAGGGG GGTGCACCAA AGAGTCCAAA
  721 ACGATCGGGA TTTTTGGACT CAGGTCGGGC CACAAAAACG GCCCCCGAAG TCCCTGGGAC
  781 GTCTCCCAGG GTTGCGGCCG GGTGTTCCGA ACTCGTCAGT TCCACCACGG GTCCGCCAGA
  841 TACAGAGCTA GTTAGCTAAC TAGTACAGAC GCAGGCGCAA ACATTAGATG CCGGCACAAA
  901 CACGCACAAA GACAGAGACA GACACGAAAC GACCGCTGGC CAGCTTACCT CCCGGCGGGG
  961 GGGGTCGGTG GTCCCTGGGC AGGGGTCTCC AAATCTCGGT GGAACCTCCA AATGAAAGAC
 1021 CCCCGGAGTG GGCAGTCAAT CAATCTGAGG AGACCCTCCC AAGGATCAGC GAGTCACGAT
 1081 CGGATGCAAC AGCAAGAGCT TATGGGAATA CCGGATACCG GCCGACTCAA GTTCAATTCC
1141 GGGAGAGGAA CTG
//
Primer: EGFP-C RDB12173_A6Ajf.seq
>12173_12173_A6Aj_EGFP-C_E07_13.ab1
    1 CGGTGCTCTC GGCATGGACG AGCTGTACAA GTAAAGCGGC CGGCCGCCAG CACAGTGGTC
   61 GACCGGGCCG CCAGCACAGT GGTCGATCGA GGGGGGGCCC GCGATTAGTC CAATTTGTTA
  121 AAGACAGGAT ATCAGTGGTC CAGGCTCTAG TTTTGACTCA ACAATATCAC CAGCTGAAGC
  181 CTATAGAGTA CGAGCCATAG ATAGAATAAA AGATTTTATT TAGTCTCCAG AAAAAGGGGG
  241 GAATGAAAGA CCCCACCTGT AGGTTTGGCA AGCTAGCTTA AGTAACGCCA TTTTGCAAGG
  301 CATGGAAAAT ACATAACTGA GAATAGAGAA GTTCAGATCA AGGTTAGGAA CAGAGAGACA
  361 GCAGAATATG GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG CTCAGGGCCA
  421 AGAACAGTTG GAACAGCAGA ATATGGGCCA AACAGGATAT CTGTGGTAAG CAGTTCCTGC
  481 CCCGGCTCAG GGCCAAGAAC AGATGGTCCC CAGATGCGGT CCCGCCCTCA GCAGTTTCTA
  541 GAGAACCATC AGATGTTTCC AGGGTGCCCC AAGGACCTGA AATGACCCTG TGCCTTATTT
  601 GAACTAACCA ATCAGTTCGC TTCTCGCTTC TGTTCGCGCG CTTCTGCTCC CCGAGCTCAA
  661 TAAAAGAGCC CACAACCCCT CACTCGGCGC GCCAGTCCTC CGATAGACTG CGTCGCCCGG
  721 GTACCCGTGT ATCCAATAAA CCCTCTTGCA GTTGCATCCG ACTTGTGGTC TCGCTGTTCC
  781 TTGGGAGGGT CTCCTCTGAG TGATTGACTA CCCGTCAGCG GGGGTCTTTC ACATGCAGCA
  841 TGTATCAAAA TTAATTTGGT TTTTTTTCTT AAGTATTTAC ATTAAATGGC CATAGTTGCA
  901 TATGATCGGC CACGCGGCGG GGGAGAGGCG GTTGCGTATG GGCGCTCTTT CGCTCCTCCG
  961 CTCACTTGAC TCGCTGCGCT CGTCGGTCCG GCTGCGGCGA GCGGCTATCA GCTCACCTCA
 1021 GCGTATCCGG TTATCCAGGA TCAGGATACC GAGAGACCTG TGAGCTATGA TCGTAGCTGA
1081 CCGTAGCCGA TCGTGGCATT TCCTG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

2019.08.06

GNP_filter3_RDBDEP_html_190432.pl