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pMYs-Tead2FL-IRES-EGFP (#RDB12173)

Retroviral vector of mouse Tead2

Clone info. Retroviral vector of mouse Tead2 full length (FL). cDNA fragment 87 to 1,424 nt of NM_011565.2 (CDS, 87..1424) is cloned.
Comment Transfection can be confirmed by GFP. See Fig. 2B of Ota and Sasaki, Development 135: 4059-4069 (2008). Known differences: G to A substitution at 453 nt (Val to Ile at 123rd aa).
Vector backbone pMYs-IRES-EGFP (Plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r
Gene/insert name Mouse Tead2 cDNA
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infections virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12173 pMYs-Tead2FL-IRES-EGFP DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMYs-Tead2FL-IRES-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12173).

Reference section:

Ota, M., Sasaki, H., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19898_B3Bap1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region,insert 5'
Sequence file: RDB19898_B3Baa.seq check
>12173_19898_B3Ba_3_MMLV-pol_F_B04_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGG NNTCGCAGCT TGGNNACNCG CCGCCCACGT GAAGGCTGCC
   61 GACCCCGGGG GTGGACCATC CTCTAGACTG CCGGATCTAG CTAGTTAATT AAGGATCCCA
  121 GTGTGGTGGT ACGGGAATTC GAGGCCCAGA TGGGGGATCC CCGGACTGGG GCCCCCCTGG
  181 ATGATGGCGG GGGCTGGACA GGTAGCGAGG AAGGCAGCGA AGAGGGCACC GGAGGCAGCG
  241 AGGGCGTTGG AGGTGATGGC AGCCCTGATG CAGAAGGTGT GTGGAGCCCC GACATTGAGC
  301 AGAGTTTTCA GGAGGCCCTG GCCATCTACC CTCCCTGTGG CCGTCGCAAG ATCATCCTGT
  361 CAGATGAGGG CAAGATGTAT GGCCGGAACG AACTCATTGC CCGTTACATC AAGCTGAGGA
  421 CAGGGAAGAC GAGAACGCGA AAGCAGGTCT CCAGCCATAT TCAGGTTTTG GCTCGAAGGA
  481 AATCGAGAGA AATTCAGTCC AAGCTGAAGG ACCAAATCTC CAAGGACAAG GCCTTCCAGA
  541 CGATGGCCAC CATGTCTTCG GCACAGCTCA TCTCCGCCCC TTCCCTCCAG GCCAAGCTGG
  601 GCCCTTCTGG CCCTCAGGCC ACTGAGCTTT TCCAGTTCTG GTCAGGGAGC TCTGGGCCAC
  661 CATGGAATGT TCCAGACGTG AAGCCCTTCT CACAGGCACC GTTCTCCGTG TCACTGACGC
  721 CNCCAGCCTC TGACCTACCA GGGTACGAGC CGCCCCCAGC CCTCTCACCC CTGCCCCCAC
  781 CCGCTNCGTC TCCCCCAGCC TGGCANGCTC NGGCCCTGGG CACTGCCCGC CTGCANCTGA
  841 NAGANTTCTC AGCGTTTGTG GAANCGCCAG ANNCANNTNN NTCNTTNCAN ANNNNTCTGN
901 TTGNNCNCAT CANNCAGNNN NNNNNNN
//
Primer: Tead2FL-B_F (Pr1296)
Region: insert 3',IRES2
Sequence file: RDB19898_B3Bab.seq check
>12173_19898_B3Ba_3_Tead2FL-B_F_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCNNN NNNAGGTCTG CTCCTTTGGC AAGCAAGTGG TAGAGAAGGT
   61 GGAGACGGAA CGGGCCCAGC TGGAGGACGG GCGCTTTGTG TACCGTCTGC TGCGCTCTCC
  121 CATGTGTGAG TACCTGGTTA ATTTCCTGCA CAAGCTCCGT CAGCTGCCTG AACGCTACAT
  181 GATGAACAGT GTCCTGGAGA ACTTCACCAT CCTCCAGGTT GTGACAAACA GGGACACTCA
  241 GGAACTGCTG CTGTGTACTG CCTACGTCTT TGAAGTCTCC ACCAGTGAAC GAGGAGCCCA
  301 GTACCACATC TACCGCCTGG TCAGGGACTG ATCTCGAGGG CCGGCGCGCC GCGGCCGCTA
361 CGTAAATTCC GCCCCCCCCC CNNNNNNNNN N
//
Primer: IRES-F (Pr0036)
Region: IRES2,EGFP 5'
Sequence file: RDB19898_B3Bac.seq check
>12173_19898_B3Ba_3_IRES-F_D03_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTTN NGTCNAGGTN NNAAAAACGT CTAGGCCCCC CGAACCACGG
   61 GGACGTGGTT TTCCTTTGAA AAACACGATG ATAATATGGC CACAACCATG GTGAGCAAGG
  121 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG
  181 GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC
  241 TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC
  301 TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT
  361 TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG
  421 GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG
  481 AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA
  541 ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA
  601 ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC
  661 AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC
  721 AGTCCGNCCT GANCAAAGAN NNCNACGAGA AGCGCGATCA CNTGGTCCTG CTGNAGTTCG
  781 TGANCGCCGC CGGGNTCACT CTCNGNNTGG NNNANCTGNN CNNGTAAAGN GGNCGGNCGC
  841 CAGCACANNN NNCNANNGGG NCGNCNGCNN ANNGGNCNAT CGAGGGGGGG NCNNNGANTA
901 NNCCNNTTNG NNNAANANNG NNNNTNNNNN NNNNAGGNNC NANTTTTGAN NNNNNNNNN
//
Primer: Amp_R (Pr0008)
Region: AmpR pro,5' LTR,psi
Sequence file: RDB19898_B3Bad.seq check
>12173_19898_B3Ba_3_Amp_R_E03_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN TTGNAGNNTT TATNNGGGTT ATTGTCTCNT GAGCGGATAC
   61 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA
  121 GTGCCACCTG CATAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT TAAGTAACGC
  181 CATTTTGCAA GGCATGGAAA AATACATAAC TGAGAATAGA AAAGTTCAGA TCAAGGTCAG
  241 GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC AGTTCCTGCC
  301 CCGGCTCAGG GCCAAGAACA GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG
  361 TAAGCAGTTC CTGCCCCGGC TCAGGGCCAA GAACAGATGG TCCCCAGATG CGGTCCAGCC
  421 CTCAGCAGTT TCTAGAGAAC CATCAGATGT TTCCAGGGTG CCCCAAGGAC CTGAAATGAC
  481 CCTGTGCCTT ATTTGAACTA ACCAATCAGT TCGCTTCTCG CTTCTGTTCG CGCGCTTCTG
  541 CTCCCCGAGC TCAATAAAAG AGCCCACAAC CCCTCACTCG GCGCGCCAGT CCTCCGATTG
  601 ACTGAGTCGC CCGGGTACCC GTATTCCCAA TAAAGCCTCT TGCTGTTTGC ATCCGAATCG
  661 TGGACTCGCT GATCCTTGGG AGGGTCTCCT CAGATTGATT GACTGCCCAC CTCGGGGGTC
  721 TTTCATTTGG AGGTTCCACC GAGATTTGGA GACCCCTGCC CAGGGNCCAC CGACCCCCCC
  781 GCCGGGAGGT AAGCTGGNCA GCGGTCGTTT CGTGTCTGTC TCTGTCTTTG TGCGTGTTTG
  841 TGNCGGCATC TAATGNTTGC GCCTGCGTCN GTNCTANTTA GCTNACTAGC TCTGTATCTG
  901 GNGNANCCGT GNNGGAACTG ANNANTTCGG AACACCCGGN NGCNNCCCTG GGNNANGNCC
  961 CNNGNANTTC NGGGGNNNNT TTTGNGGNCC GANNNNANNN NNAAATCCCN ATNGNTTTNN
1021 NNNNNTNNNN GNNNCCCCNN NNNNNNNN
//
Primer: pMFG_1 (Pr0230)
Region: pol region,psi
Sequence file: RDB19898_B3Bae.seq check
>12173_19898_B3Ba_3_pMFG_1_D04_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT NANTNNNTCT TGTNNTGTCN GGGTCAGGGA AGTTTACNAG
   61 GGGCGGGGGT GCCCCATATA AGATCTCATA TGGCCATATG GGGGCGCCTA GAGAAGGAGT
  121 GAGGGCTGGA TAAAGGGAGG ATCGAGGCGG GGTCGAACGA GGAGGTTCAA GGGGGAGAGA
  181 CGGGGCGGAT GGAGGAAGAG GAGGCGGAGG CTTAGGGTGT ACAAAGGGCT TGACCCAGGG
  241 AGGGGGGTCA AAAGCCAAGG CTTCCCAGGT CACGATGTAG GGGACCTGGT CTGGGTGTCC
  301 ATGCGGGCCA GGTGAAAAGA CCTTGATCTT AACCTGGGTG ATGAGGTCTC GGTTAAAGGT
  361 GCCGTCTCGC GGCCATCCGA CGTTAAAGGT TGGCCATTCT GCAGAGCAGA AGGTAACCCA
  421 ACGTCTCTTC TTGACATCTA CCGACTGGTT GTGAGCGATC CGCTCGACAT CTTTCCAGTG
  481 ACCTAAGGTC AAACTTAAGG GAGTGGTAAC AGTCTGGCCC TAATTTTCAG ACAAATACAG
  541 AAACACAGTC AGACAGAGAC AACACAGAAC GATGCTGCAG CAGACAAGAC GCGCGGCGCG
  601 GCTTCNGTCC CNAACCGAAA GCAAAAATTC NGACNGANGC GGGAACTGTT TTANGTTCTC
  661 GTCTNCTANN NGANNCACNT ATCCNTCCTA TTNNGGGGGN GNANCNNAGA NTCCAAAACG
  721 ATCGGGANTT TTGGANTCNN NCNGGNNNCN AAAACNGNCN NCNANNTCCN TGGGNNGNCT
781 CCNNNNNNNN NN
//
Primer: mVenus-M_F (Pr0574)
Region: EGFP 3',3' LTR
Sequence file: RDB19898_B3Baf.seq check
>12173_19898_B3Ba_3_mVenus-M_F_G04_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNN TCNNGGTNAN TTCNNGANCC GCCNCAACAT CGAGGACGGC
   61 AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG
  121 CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG
  181 CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC
  241 GAGCTGTACA AGTAAAGCGG CCGGCCGCCA GCACAGTGGT CGACCGGGCC GCCAGCACAG
  301 TGGTCGATCG AGGGGGGGCC CGCGATTAGT CCAATTTGTT AAAGACAGGA TATCAGTGGT
  361 CCAGGCTCTA GTTTTGACTC AACAATATCA CCAGCTGAAG CCTATAGAGT ACGAGCCATA
  421 GATAGAATAA AAGATTTTAT TTAGTCTCCA GAAAAAGGGG GGAATGAAAG ACCCCACCTG
  481 TAGGTTTGGC AAGCTAGCTT AAGTAACGCC ATTTTGCAAG GCATGGAAAA TACATAACTG
  541 AGAATAGAGA AGTTCAGATC AAGGTTAGGA ACAGAGAGAC AGCAGAATAT GGGCCAAACA
  601 GGATATCTGT GGTAAGCAGT TCCTGNCCCG GCTCANGGCC NAGAACAGTT GGAACAGCAG
  661 AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCN NGNCCAAGAA
  721 CAGATGGTCC CCAGATGCGG NCCCGCCCTC ANCANTTTCT AGANAANCAT CANATGNTTC
  781 NNGGGNGNCC CNNNNACCTG AAATGANCNT GNNNNTTATT TGANNTNNCN NANCANNNCG
  841 NTTCNCGNTT CNNNNCNNNN GNNNNNGNNN NNCNANNNNN NNNAAANANN NNNNNANNNN
901 NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. [link to RRC of NBRP]