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pMYs-Tead2FL-IRES-EGFP

Retroviral vector of mouse Tead2

Catalog number RDB12173
Resource name pMYs-Tead2FL-IRES-EGFP
Clone info. Retroviral vector of mouse Tead2 full length (FL). cDNA fragment 87 to 1,424 nt of NM_011565.2 (CDS, 87..1424) is cloned.
Comment Transfection can be confirmed by GFP. See Fig. 2B of Ota and Sasaki, Development 135: 4059-4069 (2008). Known differences: G to A substitution at 453 nt (Val to Ile at 123rd aa).
Vector backbone pMYs-IRES-EGFP (Plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r
Gene/insert name Mouse Tead2 cDNA
Depositor|Developer Sasaki, Hiroshi |
Other clones in our bank

External Database
Mouse Tead2

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
          Additional terms and conditions for distribution Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          Remarks Remember that you will be working with samples containing infections virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
          付加的提供条件 pMXsシリーズベクターについて:利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB12173 pMYs-Tead2FL-IRES-EGFP DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12173_A6Ajp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: 5'Retro(pFB-5')
          Region: insert5'
          Sequence file: RDB12173_A6Aja.seq check
          >12173_12173_A6Aj_5'Retro(pFB-5')_A07_01.ab1
              1 TTCAGCTGGA GGATCTATCG CGGACTGCTA GTTATTAAGG ATCCCAGTGT GGTGGTACGG
             61 GAATTCGAGG CCCAGATGGG GGATCCCCGG ACTGGGGCCC CCCTGGATGA TGGCGGGGGC
            121 TGGACAGGTA GCGAGGAAGG CAGCGAAGAG GGCACCGGAG GCAGCGAGGG CGTTGGAGGT
            181 GATGGCAGCC CTGATGCAGA AGGTGTGTGG AGCCCCGACA TTGAGCAGAG TTTTCAGGAG
            241 GCCCTGGCCA TCTACCCTCC CTGTGGCCGT CGCAAGATCA TCCTGTCAGA TGAGGGCAAG
            301 ATGTATGGCC GGAACGAACT CATTGCCCGT TACATCAAGC TGAGGACAGG GAAGACGAGA
            361 ACGCGAAAGC AGGTCTCCAG CCATATTCAG GTTTTGGCTC GAAGGAAATC GAGAGAAATT
            421 CAGTCCAAGC TGAAGGACCA AATCTCCAAG GACAAGGCCT TCCAGACGAT GGCCACCATG
            481 TCTTCGGCAC AGCTCATCTC CGCCCCTTCC CTCCAGGCCA AGCTGGGCCC TTCTGGCCCT
            541 CAGGCCACTG AGCTTTTCCA GTTCTGGTCA GGGAGCTCTG GGCCACCATG GAATGTTCCA
            601 GACGTGAAGC CCTTCTCACA GGCACCGTTC TCCGTGTCAC TGACGCCCCC AGCCTCTGAC
            661 CTACCAGGGT ACGAGCCGCC CCCAGCCCTC TCACCCCTGC CCCCACCCGC TCCGTCTCCC
            721 CCAGCCTGGC AGGCTCGGGC CCTGGGCACT GCCCGCCTGC AGCTGATAGA GTTCTCAGCG
            781 TTTGTGGAAC CGCCAGACGC AGTTGACTCG TTCCAGAGGC ATCTGTTTGT CCACATCAGT
            841 CAGCAGTGTC CCAGCCCTGG AGCACCACCC CTAGAGAGTG TGGACGTGCG GCAGATCTAC
            901 GACAAATTCC CTGAGAAGAA GGGCGGCCTC CGCGAGCTGT ATGACCGAGG GCCACCACAT
            961 GCCTTTCTTC CTCGTCAAGT TCTGGGCGGA CCTGAACTGG GGCCCCAGTG CCGAGAGGCA
           1021 GGAGCAGCGG GAGGTGGCCG TGCTTCTATG GAGGTGAGCA GCAGTAATGA GAGCAGGGAG
           1081 CTCATGACAC TTCACTGCTC TTCAAGGTCT GCTTCTTGCC AGCCAGTGGT AAGAGAAAGT
          //
          Primer: IRES-R
          Region: insert3'
          Sequence file: RDB12173_A6Ajb.seq check
          >12173_12173_A6Aj_IRES-R_H11_23.ab1
              1 GCCATCGGCG GGCTTCGGCA GTACGTTAGG GGGGGGGGAG GGAGAGGGGG GGGGGGGGCG
             61 GAATTTACGT AGCGGCCGCG GCGCGCCGGC CCTCGAGATC AGTCCCTGAC CAGGCGGTAG
            121 ATGTGGTACT GGGCTCCTCG TTCACTGGTG GAGACTTCAA AGACGTAGGC AGTACACAGC
            181 AGCAGTTCCT GAGTGTCCCT GTTTGTCACA ACCTGGAGGA TGGTGAAGTT CTCCAGGACA
            241 CTGTTCATCA TGTAGCGTTC AGGCAGCTGA CGGAGCTTGT GCAGGAAATT AACCAGGTAC
            301 TCACACATGG GAGAGCGCAG CAGACGGTAC ACAAAGCGCC CGTCCTCCAG CTGGGCCCGT
            361 TCCGTCTCCA CCTTCTCTAC CACTTGCTTG CCAAAGGAGC AGACCTTGGA GGAGCAGGTG
            421 AGTGTCATGA GCTCCCGGCT CTCATACTGG CTGCTCACTC CATAGAAGCC ACCGCCACCT
            481 CCGCTGCTCC CTGCCTCCTC GGCACTGGGG CCCCAGTTCA GGTCCGCCCA GAACTTGACG
            541 AGGAAGAAGG CATGTGGTGG CCCTCGGTCA TACAGCTCGC GGAGGCCGCC CTTCTTCTCA
            601 GGGAATTTGT CGTAGATCTG CCGCACGTCC ACACTCTCTA GGGGTGGTGC TCCAGGGCTG
            661 GGACACTGCT GACTGATGTG GACAAACAGA TGCCTCTGGA ACGAGTCAAC TGCGTCTGGC
            721 GGTTCCACAA ACGCTGAGAA CTCTATCAGC TGCAGGCGGG CAGTGCCCAG GGCCCGAGCC
            781 TGCCAGGCTG GGGGAGACGG AGCGGGTGGG GGCAGGGGTG AGAGGGCTGG GGGCGGCTCG
            841 TACCCTGGTA GGTCAGAGGC TGGGGGCGTC AGTGACACGG AGAACGGTGC CTGTGAGAAG
            901 GGCTTCACGT CTGGGAACAT TCCATGGTGG GCCCAGAGCT CCCTGACCAG AACTGGGAAA
            961 AGCTCAGTGG CCCTGAGGGC CCAGAAGGGC CCAGCTTGGC CTGAGGGAAG GGCGGGAGAT
           1021 GAGCTGTGCC GAAGACATGG TGGGCATCGT CTGAGCTTGT CCTGGAGATT GTTCTCAGCC
           1081 TGACTGAATT TCTCTCGAAT TGCATCGAGG CCGAACTGAT ATGTTGAGAA CCTGCTCTTC
          1141 G
          //
          Primer: IRES_F
          Region: EGFP 3'
          Sequence file: RDB12173_A6Ajc.seq check
          >12173_12173_A6Aj_IRES-F_A08_02.ab1
              1 GCAGTCGTCT GTGTTTAGTC GAGGTTAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG
             61 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGGTGAG CAAGGGCGAG
            121 GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC
            181 AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG
            241 TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC
            301 TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG
            361 TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC
            421 TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG
            481 AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC
            541 AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC
            601 AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC
            661 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC
            721 GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC
            781 GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT AAAGCGGCCG GGCCGCCAGC
            841 ACAGTGGTCG ACCGGGCCGC CAGCACAGTG GTCGATCGAG GGGGGGCCCG CGATTAGTCC
            901 AAATTTGTTA AAGAACAGGA TATCAGTGGT CAGCTCTAGT TTTTGACTCA ACAATATCAC
            961 CAGCTGAGGC TATAGAGTAC GAGCAGATGG ATTACGATTT ATTTAGTCTC AGAAAACGGG
           1021 GAATGGAAGA ACCCACTTGT AGTGGCAAGC TAGCCTTAGT AGCATGCACG CATGGAATAC
          1081 CTAACTTGAA G
          //
          Primer: Amp_R
          Region: 5'LTR
          Sequence file: RDB12173_A6Ajd.seq check
          >12173_12173_A6Aj_Amp_R_C07_07.ab1
              1 TTCCGTTGTT TATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
             61 TTTGAATGTA TTTAGAAAAT TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG
            121 CCACCTGCAT AATGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT
            181 TTTGCAAGGC ATGGAAAAAT ACATAACTGA GAATAGAAAA GTTCAGATCA AGGTCAGGAA
            241 CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG
            301 GCTCAGGGCC AAGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA
            361 GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCAGCCCTC
            421 AGCAGTTTCT AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT
            481 GTGCCTTATT TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC
            541 CCCGAGCTCA ATAAAAGAGC CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATTGACT
            601 GAGTCGCCCG GGTACCCGTA TTCCCAATAA AGCCTCTTGC TGTTTGCATC CGAATCGTGG
            661 ACTCGCTGAT CCTTGGGAGG GTCTCCTCAG ATTGATTGAC TGCCCACCTC GGGGGTCTTT
            721 CATTTGGAGG TTCCACCGAG ATTTGGAGAC CCCTGCCCAG GGACCACCGA CCCCCCCGCC
            781 GGGAGGTAAG CTGGCCAGCG GTCGTTTCGT GTCTGTCTCT GTCTTTGTGC GTGTTTGTGC
            841 CGGCATCTAA TGTTTGCGCC TGCGTCTGTA CTAGTTAGCT AACTAGCTCT GTATCTGGCG
            901 GACCCGTGGT GGAACTGACG AGTTCGGAAC ACCCGGGCCG CAACCCTGGG AGACGTCCCA
            961 GGGACTTCGG GGGGCCGTTT TTGTGGCCCG ACCTGAGTCC AAAAATCCCG ATCGTTTTGG
           1021 ACTCTTTTGG TGCACCCCCC CTATAGAGGA TATGTGGTTC TGGTAGGAGA CGAGACTAAA
           1081 CAGTTTCCCG CCTCCGTCTG ATTTTGCTTC GAATGGACGA GACTGGATGC GTCGTAGCTT
          1141 GCTGTGTGCA G
          //
          Primer: pMFG-1
          Region: psi
          Sequence file: RDB12173_A6Aje.seq check
          >12173_12173_A6Aj_pMFG-1_D07_10.ab1
              1 GCTCAGGGGG GGAGGAGGGG CTGTTAGTAC TCTTGTCATG TCAGGGTCAG GGAAGTTTAC
             61 AAGGGGCGGG GGTGCCCCAT ATAAGATCTC ATATGGCCAT ATGGGGGCGC CTAGAGAAGG
            121 AGTGAGGGCT GGATAAAGGG AGGATCGAGG CGGGGTCGAA CGAGGAGGTT CAAGGGGGAG
            181 AGACGGGGCG GATGGAGGAA GAGGAGGCGG AGGCTTAGGG TGTACAAAGG GCTTGACCCA
            241 GGGAGGGGGG TCAAAAGCCA AGGCTTCCCA GGTCACGATG TAGGGGACCT GGTCTGGGTG
            301 TCCATGCGGG CCAGGTGAAA AGACCTTGAT CTTAACCTGG GTGATGAGGT CTCGGTTAAA
            361 GGTGCCGTCT CGCGGCCATC CGACGTTAAA GGTTGGCCAT TCTGCAGAGC AGAAGGTAAC
            421 CCAACGTCTC TTCTTGACAT CTACCGACTG GTTGTGAGCG ATCCGCTCGA CATCTTTCCA
            481 GTGACCTAAG GTCAAACTTA AGGGAGTGGT AACAGTCTGG CCCTAATTTT CAGACAAATA
            541 CAGAAACACA GTCAGACAGA GACAACACAG AACGATGCTG CAGCAGACAA GACGCGCGGC
            601 GCGGCTTCGG TCCCAAACCG AAAGCAAAAA TTCAGACGGA GGCGGGAACT GTTTTAGGTT
            661 CTCGTCTCCT ACCAGAACCA CATATCCCTC CTATTAGGGG GGTGCACCAA AGAGTCCAAA
            721 ACGATCGGGA TTTTTGGACT CAGGTCGGGC CACAAAAACG GCCCCCGAAG TCCCTGGGAC
            781 GTCTCCCAGG GTTGCGGCCG GGTGTTCCGA ACTCGTCAGT TCCACCACGG GTCCGCCAGA
            841 TACAGAGCTA GTTAGCTAAC TAGTACAGAC GCAGGCGCAA ACATTAGATG CCGGCACAAA
            901 CACGCACAAA GACAGAGACA GACACGAAAC GACCGCTGGC CAGCTTACCT CCCGGCGGGG
            961 GGGGTCGGTG GTCCCTGGGC AGGGGTCTCC AAATCTCGGT GGAACCTCCA AATGAAAGAC
           1021 CCCCGGAGTG GGCAGTCAAT CAATCTGAGG AGACCCTCCC AAGGATCAGC GAGTCACGAT
           1081 CGGATGCAAC AGCAAGAGCT TATGGGAATA CCGGATACCG GCCGACTCAA GTTCAATTCC
          1141 GGGAGAGGAA CTG
          //
          Primer: EGFP-C
          Region: 3'LTR
          Sequence file: RDB12173_A6Ajf.seq check
          >12173_12173_A6Aj_EGFP-C_E07_13.ab1
              1 CGGTGCTCTC GGCATGGACG AGCTGTACAA GTAAAGCGGC CGGCCGCCAG CACAGTGGTC
             61 GACCGGGCCG CCAGCACAGT GGTCGATCGA GGGGGGGCCC GCGATTAGTC CAATTTGTTA
            121 AAGACAGGAT ATCAGTGGTC CAGGCTCTAG TTTTGACTCA ACAATATCAC CAGCTGAAGC
            181 CTATAGAGTA CGAGCCATAG ATAGAATAAA AGATTTTATT TAGTCTCCAG AAAAAGGGGG
            241 GAATGAAAGA CCCCACCTGT AGGTTTGGCA AGCTAGCTTA AGTAACGCCA TTTTGCAAGG
            301 CATGGAAAAT ACATAACTGA GAATAGAGAA GTTCAGATCA AGGTTAGGAA CAGAGAGACA
            361 GCAGAATATG GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG CTCAGGGCCA
            421 AGAACAGTTG GAACAGCAGA ATATGGGCCA AACAGGATAT CTGTGGTAAG CAGTTCCTGC
            481 CCCGGCTCAG GGCCAAGAAC AGATGGTCCC CAGATGCGGT CCCGCCCTCA GCAGTTTCTA
            541 GAGAACCATC AGATGTTTCC AGGGTGCCCC AAGGACCTGA AATGACCCTG TGCCTTATTT
            601 GAACTAACCA ATCAGTTCGC TTCTCGCTTC TGTTCGCGCG CTTCTGCTCC CCGAGCTCAA
            661 TAAAAGAGCC CACAACCCCT CACTCGGCGC GCCAGTCCTC CGATAGACTG CGTCGCCCGG
            721 GTACCCGTGT ATCCAATAAA CCCTCTTGCA GTTGCATCCG ACTTGTGGTC TCGCTGTTCC
            781 TTGGGAGGGT CTCCTCTGAG TGATTGACTA CCCGTCAGCG GGGGTCTTTC ACATGCAGCA
            841 TGTATCAAAA TTAATTTGGT TTTTTTTCTT AAGTATTTAC ATTAAATGGC CATAGTTGCA
            901 TATGATCGGC CACGCGGCGG GGGAGAGGCG GTTGCGTATG GGCGCTCTTT CGCTCCTCCG
            961 CTCACTTGAC TCGCTGCGCT CGTCGGTCCG GCTGCGGCGA GCGGCTATCA GCTCACCTCA
           1021 GCGTATCCGG TTATCCAGGA TCAGGATACC GAGAGACCTG TGAGCTATGA TCGTAGCTGA
          1081 CCGTAGCCGA TCGTGGCATT TCCTG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

          2022.07.13

          GNP_filter3_RDBDEP_html_220713.pl