Please review the QC test results indicated by icon below as well as clone information before placing your order.

pcDNA3.1-pA83-Tead4EnR

Expression clone of mouse Tead4, repressor-modified form

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13966_A6Bmp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro,insert 5'(Tead4,EnR) Sequence file: RDB13966_A6Bma.seq
>D03261A2_A6Bm_3_CMV-Forward_E01_13.ab1     1 AGGGTTACAC CCCCGCCCCA ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC    61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT   121 AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGTTAA GCTTATGGTA CCATGTACCC   181 ATACGACGTT CCAGATTACG CTGGTACCTC CAACGAGTGG AGCTCTCCCG ACTCCCCCGA   241 GGGGAGCAGC ATCTCTGGGG GCAGCCAGGC ACTGGACAAG CCCATCGACA ATGATGCAGA   301 GGGTGTATGG AGCCCCGAAA TTGAGCGAAG CTTCCAGGAG GCCCTGGCCA TCTACCCACC   361 CTGCGGCCGC CGCAAAATTA TCCTGACGGA GGAAGGCAAG ATGTATGGTC GGAATGAGCT   421 GATCGCACGC CATATCAAGC TCAGGACAGG GAAGACGCGC ACAAGGAAGC AGGTCTCCAG   481 CCACATCCAG GTGCTTGCCC GTCGAAAAGC CCGGGAGATC CAGGCCAAAC TCAAGGACCA   541 GGCAGCTAAG AACAAGGCCC TGCAGAGCAT GGCTGCCATG TCGTCCGCAC AGATCGTCTC   601 GGCCACAGCC TTCCACAGTA AAATGGCTCT TGCCCGGGGC CCTGGCTACC CAATCGATGC   661 CCTGGAGGAT CGCTGCAGCC CACAGTCAGC GCCCAGCCCC ATTACCCTAC AAATGCAGCA   721 TCTTCACCAC CAGCAACAGC AGCAGCAGCA ACAGCAGCAG CAAATGCAGC ACCTCCACCA   781 GCTGCAGCAA CTGCAGCAGT TGCACCAACA GCAACTGGCC GCCGGTGTCT TCCACCATCC   841 GGCAATGGCC TTCGATGCCG CTGCAGCCGC CGCTGCTGCA GCTGCTGCTG CGGCCGCCCA   901 CGCTCATGCT GCTGCACTGC AGCAGCGCCC TCAGTGGCAG TGGATCGCCC GCATCCTGCT   961 CCACGCCCGC CCTCGTCCAC GCCCGCTGGA CCATCAAGGA GGAGGAAAGG CGACCTCCGG  1021 TGAATCGGTG ACAATGGAGT TTCCACAAAT CAGAACGGCA ACCACCCACA ACGAGGAGGA  1081 GGAAGGCGGA GGGAGGAATG ACGAACATGA ATGTGAGTGT GGATGGATAC CGTCCGCGGG 1141 CGGAACGCCG TGGCAACATC CGGTCCCAAC CAGACCAGCA GTTCAGAACA GC //
Primer: GNP Primer #10 Region: TRF2-3'UTR pA,insert 3'(EnR) Sequence file: RDB13966_A6Bmb.seq
>D03261A2_A6Bm_3_GNPprimer#10_F01_16.ab1     1 TTTAATTGGT TCCAATTAAT TTAAAAAGAT AACGTTTACA GGTTCTTTGG TGCCCATAGT    61 CGAGCGGCCG CCACTGTGCT GGATATCTGC AGAATTCGGA TCCCTAGTTC AGGTCCTCCT   121 CGGAGATCAG CTTCTGCTCA AGCTTGGGCT GCATAGATCC CAGAGCAGAT TTCTCTGGAT   181 TGGCATCGCT CATCTTGGAG CTCATGGGCG GCGGGATGAC GGCATTGGCT GCACTCCTGC   241 GCAGGCGACT CTGCACGATT TCCTCGTGTA TCCTGGGATA GGCAGCCGGA TTGAAGCAGT   301 TAAGGAAGTT GGCCCGTTCC AGCATCATGG CCGCAGTAGC GGCTGCGGCG GCAGCTTGCT   361 GTTGCCGGCT AAACTCCAGC AGATCCACTC TTGTAAAGGC GGAGGGCGTG GCAGTCTGGG   421 AGCGATTCGC CTCGAAGGGG CGGAATATGC TGGCCTGGTT CTCAATCGAC TTGCCAGGCT   481 TCTGGACATC TCCGAAACGA TCGCTCAGGA TGTTGGAGAT GGAAAAGGCC AGCGAGGGCT   541 TGGCCGTCGA CTGCTGCTGG TGGGCGGGTG GTGGCAGGCG TCCGCCCGCC GACGTATCAT   601 CCACATCCAC ATCAATGTCG TCATCCTCCT CCGCCTCCTC CTCCTCGTTG GTGGTGTGCG   661 TCTGATTGTG GAAAACTCAT GTCACCGATC ACGGAGTCGC TTTCCTCCTC CTTGATGGTC   721 AGCGGGCGTG GACGAGGCGG GCGTGGAGCA GGATGCGGGC GATCCACTGC CACTGAGGCG   781 CTGCTGCAGT GCAGCAGCAT GAGCGTGGGG CGGCCGCAGC AGCAGCTGCA GCAGCGGGCG   841 GCTGCAGCGG GCATCGAAAG GCCAATTGCC GGGATGGTGG AAGACACCCG GCGGCCCAGT   901 TTGCTGTTTG GTGGCAACTG CTGCAGTTGC TGCAGCTGGT TGGAAGGTGG CTGCATTTGC   961 TGCCTGCTGT TTGCTGCTGC TGCTGTTGCT GGTGGGTGAA GATGCTGCAT TTGTAGGGTA  1021 AATGGGGCTG GGCGCCTGAC TGTGGGCTGC AGCGATCCTT CCGAGGCATT CGATTGGGGT  1081 AGCCAGGGGC CCGGGGCCAG AGTCAATTTT AACTGGTGAA GGCCTGTGTC CGAAAAACTA  1141 TCTGTGGCGG ACAACTGGGC AACCAATCCT CTGGTGCAAG GACCTGTGGT TTCTTTTGAC 1201 CGTGCGCTCG T //
Primer: BGH_rev2 Region: bGHpA,TRF2-3'UTR pA Sequence file: RDB13966_A6Bmc.seq
>D03261A2_A6Bm_3_BGH_rev2_G01_19.ab1     1 CACCGGGGTC AGGGAAGGCA CGGGGGAGGG GCAAACAACA GATGGCTGGC AACTAGAAGG    61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC TCAATGGTGA TGGTGATGAT GACCGGTATG   121 CATATTCAGA TCCTCTTCTG AGATGAGTTT TTGTTCGAAG GGCCCTCTAG ACTCGAGGTA   181 CGCCACAGCG TATGATGCGT ATTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT   241 TCTTTTCCAA ACTTTTATTT GTGCACATTC AATTTAAAAA AATAACGTTT ACAGGTTCTT   301 TGGTGCCCAT AGTCAAGCGG CCCCCCCTGT GCTGGATATC TGCAAAATTC GGATCCCTAT   361 TTCAGGTCCC CCTCGGAAAT CAGCTTCTGC TCAAGCTTGG GCTGCATAAA TCCCAAAGCA   421 AATTTCTCTG GATTGGCATC CCTCATCTTG GAGCTCATGG GCGGCGGGAT GACGGCATTG   481 GCTGCACTCC TGCGCAGGCA ACTCTGCACA ATTTCCTCGT GTATCCTGGG ATAGGCACCC   541 GGATTGAACC ATTTAAGGAA GTTGGCCCGT TCCACCATCA TGGCCGCAGT AACGGCTGCG   601 GCGGCAGCTT GCTGTTGCCG GCTAACTCCA GCAAATCACT CTTGTAAGGC GGAGGCGTGG   661 CAGTCTGGAA CAATTCCCTC GAAGGGCGAT ATGCTGGCCT GTCTCATCGA CTTGCAGCTC   721 GGACATCCGA ACATCGCTCA GATGTGAATG AAAGCACGAG GCTGCCGTCA CTGCTGCTGT   781 GGCGGTGTGC AGCGTCGCGC GACGACATCA ATCAACATCA TGTCGTCATC CTCCTCCGCC   841 TCTCCTCTCG TTGTGTTCGC TGATTGGATC CAAGTAACGA TACGAATGCG TACTCTTGAT   901 GTCCACGGCT GCCACGCGCT GAGACGATGC GGCGATCATG CATAGGCCGT GCATGCACGA   961 GTGACGTGGG GCAGACACGC TGCACACGCG CTCACGCATC GAGGCAATGC CGATGTGACG 1021 CG //
Primer: SV40pro_F Region: SV40 pro_ori,NeoR Sequence file: RDB13966_A6Bmd.seq
>D03261A2_A6Bm_3_SV40pro_F_H01_22.ab1     1 CCCTGTTCCC TTGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA    61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC   121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG   181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG   241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA   301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC   361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC   421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA   481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA   541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT   601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT   661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT   721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA   781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA   841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG   901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG CGAAATGACC GACCAAGCGA   961 CGCCCAACCT GCCATCACGA GATTTCGATT CACGCCGCTT CTATGAAGTT GGGCCTCCGG  1021 ATCGTTTTCA GGACGCCGCT GGATGATCTC AGCGCGGGGG ATCTCATGCT GAGTCCTCGC  1081 CAACCCAACC TTGTTATGCA GCTATAATGG TTACACTAAG GCATTAGCAT CACATTTCAC 1141 AAATTAAAAG C //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl