Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3.1-pA83-Tead4EnR (#RDB12187)

Expression clone of mouse Tead4, repressor-modified form

Clone info. Expression clone of mouse Tead4, repressor-modified form.
Comment 1-150aa of Tead4 fused to the repression domain of Engrailed. See Fig. 2B of Nishioka et al., Dev. Cell 16: 398-410 (2009). The expression vector has a stretch of poly(A).
Vector backbone pcDNA3.1-pA83 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Mouse Tead4 cDNA Drosophila eng cDNA
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12187 pcDNA3.1-pA83-Tead4EnR DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-pA83-Tead4EnR was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12187).

Reference section:

Nishioka, N., Inoue, K., Adachi, K., Kiyonari, H., Ota, M., Ralston, A., Yabuta, N., Hirahara, S., Stephenson, R.O., Ogonuki, N., Makita, R., Kurihara, H., Morin-Kensicki, E.M., Nojima, H., Rossant, J., Nakao, K., Niwa, H., Sasaki, H., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13966_A6Bmp1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,insert 5'(Tead4,EnR)
Sequence file: RDB13966_A6Bma.seq check
>D03261A2_A6Bm_3_CMV-Forward_E01_13.ab1
    1 AGGGTTACAC CCCCGCCCCA ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT
  121 AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGTTAA GCTTATGGTA CCATGTACCC
  181 ATACGACGTT CCAGATTACG CTGGTACCTC CAACGAGTGG AGCTCTCCCG ACTCCCCCGA
  241 GGGGAGCAGC ATCTCTGGGG GCAGCCAGGC ACTGGACAAG CCCATCGACA ATGATGCAGA
  301 GGGTGTATGG AGCCCCGAAA TTGAGCGAAG CTTCCAGGAG GCCCTGGCCA TCTACCCACC
  361 CTGCGGCCGC CGCAAAATTA TCCTGACGGA GGAAGGCAAG ATGTATGGTC GGAATGAGCT
  421 GATCGCACGC CATATCAAGC TCAGGACAGG GAAGACGCGC ACAAGGAAGC AGGTCTCCAG
  481 CCACATCCAG GTGCTTGCCC GTCGAAAAGC CCGGGAGATC CAGGCCAAAC TCAAGGACCA
  541 GGCAGCTAAG AACAAGGCCC TGCAGAGCAT GGCTGCCATG TCGTCCGCAC AGATCGTCTC
  601 GGCCACAGCC TTCCACAGTA AAATGGCTCT TGCCCGGGGC CCTGGCTACC CAATCGATGC
  661 CCTGGAGGAT CGCTGCAGCC CACAGTCAGC GCCCAGCCCC ATTACCCTAC AAATGCAGCA
  721 TCTTCACCAC CAGCAACAGC AGCAGCAGCA ACAGCAGCAG CAAATGCAGC ACCTCCACCA
  781 GCTGCAGCAA CTGCAGCAGT TGCACCAACA GCAACTGGCC GCCGGTGTCT TCCACCATCC
  841 GGCAATGGCC TTCGATGCCG CTGCAGCCGC CGCTGCTGCA GCTGCTGCTG CGGCCGCCCA
  901 CGCTCATGCT GCTGCACTGC AGCAGCGCCC TCAGTGGCAG TGGATCGCCC GCATCCTGCT
  961 CCACGCCCGC CCTCGTCCAC GCCCGCTGGA CCATCAAGGA GGAGGAAAGG CGACCTCCGG
 1021 TGAATCGGTG ACAATGGAGT TTCCACAAAT CAGAACGGCA ACCACCCACA ACGAGGAGGA
 1081 GGAAGGCGGA GGGAGGAATG ACGAACATGA ATGTGAGTGT GGATGGATAC CGTCCGCGGG
1141 CGGAACGCCG TGGCAACATC CGGTCCCAAC CAGACCAGCA GTTCAGAACA GC
//
Primer: GNP Primer #10 (Pr0763)
Region: TRF2-3'UTR pA,insert 3'(EnR)
Sequence file: RDB13966_A6Bmb.seq check
>D03261A2_A6Bm_3_GNPprimer#10_F01_16.ab1
    1 TTTAATTGGT TCCAATTAAT TTAAAAAGAT AACGTTTACA GGTTCTTTGG TGCCCATAGT
   61 CGAGCGGCCG CCACTGTGCT GGATATCTGC AGAATTCGGA TCCCTAGTTC AGGTCCTCCT
  121 CGGAGATCAG CTTCTGCTCA AGCTTGGGCT GCATAGATCC CAGAGCAGAT TTCTCTGGAT
  181 TGGCATCGCT CATCTTGGAG CTCATGGGCG GCGGGATGAC GGCATTGGCT GCACTCCTGC
  241 GCAGGCGACT CTGCACGATT TCCTCGTGTA TCCTGGGATA GGCAGCCGGA TTGAAGCAGT
  301 TAAGGAAGTT GGCCCGTTCC AGCATCATGG CCGCAGTAGC GGCTGCGGCG GCAGCTTGCT
  361 GTTGCCGGCT AAACTCCAGC AGATCCACTC TTGTAAAGGC GGAGGGCGTG GCAGTCTGGG
  421 AGCGATTCGC CTCGAAGGGG CGGAATATGC TGGCCTGGTT CTCAATCGAC TTGCCAGGCT
  481 TCTGGACATC TCCGAAACGA TCGCTCAGGA TGTTGGAGAT GGAAAAGGCC AGCGAGGGCT
  541 TGGCCGTCGA CTGCTGCTGG TGGGCGGGTG GTGGCAGGCG TCCGCCCGCC GACGTATCAT
  601 CCACATCCAC ATCAATGTCG TCATCCTCCT CCGCCTCCTC CTCCTCGTTG GTGGTGTGCG
  661 TCTGATTGTG GAAAACTCAT GTCACCGATC ACGGAGTCGC TTTCCTCCTC CTTGATGGTC
  721 AGCGGGCGTG GACGAGGCGG GCGTGGAGCA GGATGCGGGC GATCCACTGC CACTGAGGCG
  781 CTGCTGCAGT GCAGCAGCAT GAGCGTGGGG CGGCCGCAGC AGCAGCTGCA GCAGCGGGCG
  841 GCTGCAGCGG GCATCGAAAG GCCAATTGCC GGGATGGTGG AAGACACCCG GCGGCCCAGT
  901 TTGCTGTTTG GTGGCAACTG CTGCAGTTGC TGCAGCTGGT TGGAAGGTGG CTGCATTTGC
  961 TGCCTGCTGT TTGCTGCTGC TGCTGTTGCT GGTGGGTGAA GATGCTGCAT TTGTAGGGTA
 1021 AATGGGGCTG GGCGCCTGAC TGTGGGCTGC AGCGATCCTT CCGAGGCATT CGATTGGGGT
 1081 AGCCAGGGGC CCGGGGCCAG AGTCAATTTT AACTGGTGAA GGCCTGTGTC CGAAAAACTA
 1141 TCTGTGGCGG ACAACTGGGC AACCAATCCT CTGGTGCAAG GACCTGTGGT TTCTTTTGAC
1201 CGTGCGCTCG T
//
Primer: BGH_rev2 (Pr0606)
Region: bGHpA,TRF2-3'UTR pA
Sequence file: RDB13966_A6Bmc.seq check
>D03261A2_A6Bm_3_BGH_rev2_G01_19.ab1
    1 CACCGGGGTC AGGGAAGGCA CGGGGGAGGG GCAAACAACA GATGGCTGGC AACTAGAAGG
   61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC TCAATGGTGA TGGTGATGAT GACCGGTATG
  121 CATATTCAGA TCCTCTTCTG AGATGAGTTT TTGTTCGAAG GGCCCTCTAG ACTCGAGGTA
  181 CGCCACAGCG TATGATGCGT ATTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
  241 TCTTTTCCAA ACTTTTATTT GTGCACATTC AATTTAAAAA AATAACGTTT ACAGGTTCTT
  301 TGGTGCCCAT AGTCAAGCGG CCCCCCCTGT GCTGGATATC TGCAAAATTC GGATCCCTAT
  361 TTCAGGTCCC CCTCGGAAAT CAGCTTCTGC TCAAGCTTGG GCTGCATAAA TCCCAAAGCA
  421 AATTTCTCTG GATTGGCATC CCTCATCTTG GAGCTCATGG GCGGCGGGAT GACGGCATTG
  481 GCTGCACTCC TGCGCAGGCA ACTCTGCACA ATTTCCTCGT GTATCCTGGG ATAGGCACCC
  541 GGATTGAACC ATTTAAGGAA GTTGGCCCGT TCCACCATCA TGGCCGCAGT AACGGCTGCG
  601 GCGGCAGCTT GCTGTTGCCG GCTAACTCCA GCAAATCACT CTTGTAAGGC GGAGGCGTGG
  661 CAGTCTGGAA CAATTCCCTC GAAGGGCGAT ATGCTGGCCT GTCTCATCGA CTTGCAGCTC
  721 GGACATCCGA ACATCGCTCA GATGTGAATG AAAGCACGAG GCTGCCGTCA CTGCTGCTGT
  781 GGCGGTGTGC AGCGTCGCGC GACGACATCA ATCAACATCA TGTCGTCATC CTCCTCCGCC
  841 TCTCCTCTCG TTGTGTTCGC TGATTGGATC CAAGTAACGA TACGAATGCG TACTCTTGAT
  901 GTCCACGGCT GCCACGCGCT GAGACGATGC GGCGATCATG CATAGGCCGT GCATGCACGA
  961 GTGACGTGGG GCAGACACGC TGCACACGCG CTCACGCATC GAGGCAATGC CGATGTGACG
1021 CG
//
Primer: SV40pro_F (Pr0110)
Region: SV40 pro_ori,NeoR
Sequence file: RDB13966_A6Bmd.seq check
>D03261A2_A6Bm_3_SV40pro_F_H01_22.ab1
    1 CCCTGTTCCC TTGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG CGAAATGACC GACCAAGCGA
  961 CGCCCAACCT GCCATCACGA GATTTCGATT CACGCCGCTT CTATGAAGTT GGGCCTCCGG
 1021 ATCGTTTTCA GGACGCCGCT GGATGATCTC AGCGCGGGGG ATCTCATGCT GAGTCCTCGC
 1081 CAACCCAACC TTGTTATGCA GCTATAATGG TTACACTAAG GCATTAGCAT CACATTTCAC
1141 AAATTAAAAG C
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. [link to RRC of NBRP]