Please review the QC test results indicated by icon below as well as clone information before placing your order.

pcDNA3.1-His-hGLI1

Expression vector of human Gli1 cDNA

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In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Development, 126, 3915-3924 (1999).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Development, 126, 3915-3924 (1999).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18390_B0I7p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,6XHis,T7 tag,Xpress tag,insert 5' Sequence file: RDB18390_B0I7a.seq
>08063_18390_B0I7_1_CMV_Forward_C02_08_ABI24.ab1     1 NNNNNNNNNN NCNCNNTTNN NGCNNTGGGC GGTANGCGTG TACGGTGGGN GGTCTATATA    61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG   121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTACC ATGGGGGGTT   181 CTCATCATCA TCATCATCAT GGTATGGCTA GCATGACTGG TGGACAGCAA ATGGGTCGGG   241 ATCTGTACGA CGATGACGAT AAGGTACCTA AGGATCCAGT GTGGTGGAAT TCTGCAGATG   301 ACGCCATGTT CAACTCGATG ACCCCACCAC CAATCAGTAG CTATGGCGAG CCCTGCTGTC   361 TCCGGCCCCT CCCCAGTCAG GGGGCCCCCA GTGTGGGGAC AGAAGGACTG TCTGGCCCGC   421 CCTTCTGCCA CCAAGCTAAC CTCATGTCCG GCCCCCACAG TTATGGGCCA GCCAGAGAGA   481 CCAACAGCTG CACCGAGGGC CCACTCTTTT CTTCTCCCCG GAGTGCAGTC AAGTTGACCA   541 AGAAGCGGGC ACTGTCCATC TCACCTCTGT CGGATGCCAG CCTGGACCTG CAGACGGTTA   601 TCCGCACCTC ACCCAGCTCC CTCGTAGCTT TCATCAACTC GCGATGCACA TCTCCAGGAG   661 GCTCCTACGG TCATCTCTCC ATTGGCACCA TGAGCCCATC TCTGGGANTC CCAGCCCAGA   721 TGAATCACCA AAAAGGGCCC TCGCCTTCCT TTGGGGTCCA GCCTTGTGGT CCCCATGACT   781 CTGCCCGGNG TGGGNTGATC CCACATCCTC AGTCCCGGGG ACCCTTCCCA ACTTGCCAGC   841 TGAANTCTGA NCTGGANATG CTGGTTGNNA NNNGCCGGNN NGAACCCTNN GAANGTGNNA   901 TGTCCNGCNN NNACTCCNCA GGCATACNNG NNNCCCNNGT TGGGGGNNGN NNNNNGGNNN 961 GNNNGNNNNN NNNNN //
Primer: bGH_rev3 (Pr0885) Region: bGHpA,insert 3' Sequence file: RDB18390_B0I7b.seq
>08063_18390_B0I7_1_BGH_rev3_D02_11_ABI24.ab1     1 NNNNNNNNNN NNNNGGNNGN NGGNNNNNNA CAGNNGGCTG GCAACTAGAA GGCACAGTCG    61 AGGCTGATCA GCGGGTTTAA ACGGGCCCTC TAGACTCGAG CGGCCGCTCT AGAACTAGTG   121 GATCCCCCGG GCTGCAGGAA TTCCCCTTTA TTATCAGGAA ACAGTGTCAT TATCAAATTT   181 CTCCTCAAAA CGTATACAGA CTATACATAG CCCCCAGCCC ATACCTCCCA TCCCTGGGGC   241 ATCTTGTGCA GGGGACTGCA GCTCCCCCAA TTTTTCTGGA AGGATAGAAA CCCCTTAGGA   301 AATGCGATCT GTGATGGATG AGATTCCCTA CTCTTTAGGC ACTAGAGTTG AGGAATTCTG   361 TTTCCCCAGG TAGGGATCTC AGTAAGACAC TCATGTTGCC CACAGCCATG TTGGGGGGCC   421 CAGAGGGAGG TGGGGTATGT CCAGAGCTGC CCCGCTGATC ATGGGAAGGA GGAGGACTCA   481 GCCCCTGGGG CTCATCCAGA ATAGCCACAA AGTCCAGCTG AGTGTTGTCA AGATCAAGAG   541 AGTCCAGGGG GTTACATACC TGTCCTTCGG GAGGAGGGTA CAAGGCAGGA CCCCCTCCTA   601 GCCTGCCCAC CTCAGGATGA CCACAGCTGG GGTTTGTGCC TCCCACTTTG AGAGGCCCAT   661 AGCAAGTGGG CAATGGGGGC AGAAGTCGAG GTGGTGCTGC TGCCCTATGT GAAGCCCTAT   721 TTGCCCCCAC TACAAAATTT TCATGGCATG GTGAAGGGGT GGGATAGGAA NCTGACTTGT   781 GATTGGGCAA GTTGGGTCCN AAGCCAGGTC CATATGTGTT CACTGGANCN NNANCACGGC   841 TTGGGCTAAC CNGGGAACCC CCCCAGAAAC TNNGGNNTCN NGNANGAANN NTNNNNGNNN 901 GGGNNNTNNT CCNNNNNCNN NCCNNNCNNC ANNANCTNNN NNNNANNNNN NNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR Sequence file: RDB18390_B0I7c.seq
>08063_183903_B0I7_1_SV40pro_ori_F_E02_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC TNTNNNNNNN NNNNNNNNNN    61 GGCTTTTTTG GAGGCCTANG NTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC   121 GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC   181 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA   241 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT   301 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG   361 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA   421 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT   481 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG   541 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG   601 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC   661 GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGNNCCAT   721 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC   781 TGTGGCCGGC TGGGTGTGGC GGANCGCTAT CANGACATAG CGTTGGCTAC CCGNGATATT   841 GCTGAAGANC TTGGCGGCGA ANGGGCTGAN CGCTTCCNCG NNCNTTACNG TATCGCCGCN 901 CCCNATTCNN ANCGCATCGC NNNCNATCGC NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.02

GNP_filter3_RDBDEP_html_230501.pl