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pcDNA3.1-His-hGLI1

Expression vector of human Gli1 cDNA

Catalog number RDB08063
Resource name pcDNA3.1-His-hGLI1
Clone info. Expression vector of human Gli1 cDNA.
Vector backbone pcDNA3.1/HisB (Plasmid)
Selectable markers Amp^r
Gene/insert name Human GLI1 cDNA
Depositor Sasaki, Hiroshi |
 
Other clones in our bank human GLI1 (NCBI Gene 2735) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Development, 126, 3915-3924 (1999).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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RDB08063 pcDNA3.1-His-hGLI1 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08063_A3G9.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp | Sequence: RDB08063_A3G9a.seq
>08063_08063_A3G9_T7_20bp_E07_13.ab1
    1 CGTACCATGC GATTACTTAA GCTTACCATG GGGGGTTCTC ATCATCATCA TCATCATGGT
   61 ATGGCTAGCA TGACTGGTGG ACAGCAAATG GGTCGGGATC TGTACGACGA TGACGATAAG
  121 GTACCTAAGG ATCCAGTGTG GTGGAATTCT GCAGATGACG CCATGTTCAA CTCGATGACC
  181 CCACCACCAA TCAGTAGCTA TGGCGAGCCC TGCTGTCTCC GGCCCCTCCC CAGTCAGGGG
  241 GCCCCCAGTG TGGGGACAGA AGGACTGTCT GGCCCGCCCT TCTGCCACCA AGCTAACCTC
  301 ATGTCCGGCC CCCACAGTTA TGGGCCAGCC AGAGAGACCA ACAGCTGCAC CGAGGGCCCA
  361 CTCTTTTCTT CTCCCCGGAG TGCAGTCAAG TTGACCAAGA AGCGGGCACT GTCCATCTCA
  421 CCTCTGTCGG ATGCCAGCCT GGACCTGCAG ACGGTTATCC GCACCTCACC CAGCTCCCTC
  481 GTAGCTTTCA TCAACTCGCG ATGCACATCT CCAGGAGGCT CCTACGGTCA TCTCTCCATT
  541 GGCACCATGA GCCCATCTCT GGGATTCCCA GCCCAGATGA ATCACCAAAA AGGGCCCTCG
  601 CCTTCCTTTG GGGTCCAGCC TTGTGGTCCC CATGACTCTG CCCGGGGTGG GATGATCCCA
  661 CATCCTCAGT CCCGGGGACC CTTCCCAACT TGCCAGCTGA AGTCTGAGCT GGACATGCTG
  721 GTTGGCAAGT GCCGGGAGGA ACCCTTGGAA GGTGATATGT CCAGCCCCAA CTCCACAGGC
  781 ATACAGGATC CCCTGTTGGG GATGCTGGAT GGGCGGGAGG ACCTCGAGAG AGAGGAGAAG
  841 CGTGAGCCTG AATCTGTGTA TGAAACTGAC TGCCGTTGGG ATGGCTGCAG CCAGGAATTT
  901 GACTCCCAAG AGCAGCTGGT GCACCACATC AACAGCGAGC ACATCCACGG GGAGCGGAAA
  961 GGAGTTCGTG TGCCACTGGG GGGGGCTGCT CCAGGGAGCT GAGGCCCTTC AAAGCCCAGT
 1021 ACATGCTGGT GGTTCACATG CGCAGACACA CTGGCGAGAG CACACAAGTG CACGTTTGAT
 1081 GGTGCTGAGT CATACTCACG CCTCGAACTG AGACGCCACT GCGTCACACC ACGGGTTGAA
1141 GAAGTCAATA CCAGT
//
Primer: BGH_rev | Sequence: RDB08063_A3G9b.seq
>08063_08063_A3G9_BGH_rev_F07_16.ab1
    1 CTGTACGGGG ACTCTAGACT CGAGCGGCCG CTCTAGAACT AGTGGATCCC CCGGGCTGCA
   61 GGAATTCCCC TTTATTATCA GGAAACAGTG TCATTATCAA ATTTCTCCTC AAAACGTATA
  121 CAGACTATAC ATAGCCCCCA GCCCATACCT CCCATCCCTG GGGCATCTTG TGCAGGGGAC
  181 TGCAGCTCCC CCAATTTTTC TGGAAGGATA GAAACCCCTT AGGAAATGCG ATCTGTGATG
  241 GATGAGATTC CCTACTCTTT AGGCACTAGA GTTGAGGAAT TCTGTTTCCC CAGGTAGGGA
  301 TCTCAGTAAG ACACTCATGT TGCCCACAGC CATGTTGGGG GGCCCAGAGG GAGGTGGGGT
  361 ATGTCCAGAG CTGCCCCGCT GATCATGGGA AGGAGGAGGA CTCAGCCCCT GGGGCTCATC
  421 CAGAATAGCC ACAAAGTCCA GCTGAGTGTT GTCAAGATCA AGAGAGTCCA GGGGGTTACA
  481 TACCTGTCCT TCGGGAGGAG GGTACAAGGC AGGACCCCCT CCTAGCCTGC CCACCTCAGG
  541 ATGACCACAG CTGGGGTTTG TGCCTCCCAC TTTGAGAGGC CCATAGCAAG TGGGCAATGG
  601 GGGCAGAAGT CGAGGTGGTG CTGCTGCCCT ATGTGAAGCC CTATTTGCCC CCACTACAAA
  661 ATTTTCATGG CATGGTGAAG GGGTGGGATA GGAACCTGAC TTGTGATTGG GCAAGTTGGG
  721 TCCAAAGCCA GGTCCATATG TGTTCACTGG AGCTTTAGCA CGGCTTGGGC TAACCTGGGA
  781 ACCCCCCAGA AACTTGGGAC TCTGGTAGGA AGGTTCCTGG GCGGGGGCAT CTTCCCTGCC
  841 CCCACCCTCC CACAGTAGCT CCTGTTGAGA TTGAACATAA TTACACACAA GCTGAGCCTT
  901 GAGCTGCCCT GTGGAATGGG TGGGGGAATC AAAGTCCAGG CAAGGCCTGG GTTCTGAAGG
  961 AAGATAATCA GGGGTGGCTG GGTATAGGGG CCTGACTGGA GATATTGGGG AGGAGAGGGC
 1021 TGGGGGGTAA TGGGAAAAGA GAGGCTGTGG ATGTGGGGGC CCCCTCCACT GGGGGTGGGC
 1081 ATGGAGCAGT GCAGTCCTGT GAGTTCGAAC CCACTGGCAC CCCCTGATCT GCTGGACTGC
1141 ACTTTGCTCC ATAATTGATT CCAAG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919.
user report Mikami, Y., GLI-mediated Keratin 17 expression promotes tumor cell growth through the anti-apoptotic function in oral squamous cell carcinomas. J. Cancer Res. Clin. Oncol. 143 (8): 1381-1393 (2017). PMID 28342001.

2019.11.20

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