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pcDNA3.1-His-hGLI1 (#RDB08063)

Expression vector of human Gli1 cDNA

Clone info. Expression vector of human Gli1 cDNA.
Vector backbone pcDNA3.1/HisB (Plasmid)
Selectable markers Amp^r
Growth conditions 37C, LB+Amp
Gene/insert name Human GLI1 cDNA
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Development, 126, 3915-3924 (1999).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Development, 126, 3915-3924 (1999).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08063 pcDNA3.1-His-hGLI1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-His-hGLI1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08063).

Reference section:

Sasaki, H., Nishizaki, Y., Hui, C., Nakafuku, M., Kondoh, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18390_B0I7p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,6XHis,T7 tag,Xpress tag,insert 5'
Sequence file: RDB18390_B0I7a.seq check
>08063_18390_B0I7_1_CMV_Forward_C02_08_ABI24.ab1
    1 NNNNNNNNNN NCNCNNTTNN NGCNNTGGGC GGTANGCGTG TACGGTGGGN GGTCTATATA
   61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
  121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTACC ATGGGGGGTT
  181 CTCATCATCA TCATCATCAT GGTATGGCTA GCATGACTGG TGGACAGCAA ATGGGTCGGG
  241 ATCTGTACGA CGATGACGAT AAGGTACCTA AGGATCCAGT GTGGTGGAAT TCTGCAGATG
  301 ACGCCATGTT CAACTCGATG ACCCCACCAC CAATCAGTAG CTATGGCGAG CCCTGCTGTC
  361 TCCGGCCCCT CCCCAGTCAG GGGGCCCCCA GTGTGGGGAC AGAAGGACTG TCTGGCCCGC
  421 CCTTCTGCCA CCAAGCTAAC CTCATGTCCG GCCCCCACAG TTATGGGCCA GCCAGAGAGA
  481 CCAACAGCTG CACCGAGGGC CCACTCTTTT CTTCTCCCCG GAGTGCAGTC AAGTTGACCA
  541 AGAAGCGGGC ACTGTCCATC TCACCTCTGT CGGATGCCAG CCTGGACCTG CAGACGGTTA
  601 TCCGCACCTC ACCCAGCTCC CTCGTAGCTT TCATCAACTC GCGATGCACA TCTCCAGGAG
  661 GCTCCTACGG TCATCTCTCC ATTGGCACCA TGAGCCCATC TCTGGGANTC CCAGCCCAGA
  721 TGAATCACCA AAAAGGGCCC TCGCCTTCCT TTGGGGTCCA GCCTTGTGGT CCCCATGACT
  781 CTGCCCGGNG TGGGNTGATC CCACATCCTC AGTCCCGGGG ACCCTTCCCA ACTTGCCAGC
  841 TGAANTCTGA NCTGGANATG CTGGTTGNNA NNNGCCGGNN NGAACCCTNN GAANGTGNNA
  901 TGTCCNGCNN NNACTCCNCA GGCATACNNG NNNCCCNNGT TGGGGGNNGN NNNNNGGNNN
961 GNNNGNNNNN NNNNN
//
Primer: bGH_rev3 (Pr0885)
Region: bGHpA,insert 3'
Sequence file: RDB18390_B0I7b.seq check
>08063_18390_B0I7_1_BGH_rev3_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNGGNNGN NGGNNNNNNA CAGNNGGCTG GCAACTAGAA GGCACAGTCG
   61 AGGCTGATCA GCGGGTTTAA ACGGGCCCTC TAGACTCGAG CGGCCGCTCT AGAACTAGTG
  121 GATCCCCCGG GCTGCAGGAA TTCCCCTTTA TTATCAGGAA ACAGTGTCAT TATCAAATTT
  181 CTCCTCAAAA CGTATACAGA CTATACATAG CCCCCAGCCC ATACCTCCCA TCCCTGGGGC
  241 ATCTTGTGCA GGGGACTGCA GCTCCCCCAA TTTTTCTGGA AGGATAGAAA CCCCTTAGGA
  301 AATGCGATCT GTGATGGATG AGATTCCCTA CTCTTTAGGC ACTAGAGTTG AGGAATTCTG
  361 TTTCCCCAGG TAGGGATCTC AGTAAGACAC TCATGTTGCC CACAGCCATG TTGGGGGGCC
  421 CAGAGGGAGG TGGGGTATGT CCAGAGCTGC CCCGCTGATC ATGGGAAGGA GGAGGACTCA
  481 GCCCCTGGGG CTCATCCAGA ATAGCCACAA AGTCCAGCTG AGTGTTGTCA AGATCAAGAG
  541 AGTCCAGGGG GTTACATACC TGTCCTTCGG GAGGAGGGTA CAAGGCAGGA CCCCCTCCTA
  601 GCCTGCCCAC CTCAGGATGA CCACAGCTGG GGTTTGTGCC TCCCACTTTG AGAGGCCCAT
  661 AGCAAGTGGG CAATGGGGGC AGAAGTCGAG GTGGTGCTGC TGCCCTATGT GAAGCCCTAT
  721 TTGCCCCCAC TACAAAATTT TCATGGCATG GTGAAGGGGT GGGATAGGAA NCTGACTTGT
  781 GATTGGGCAA GTTGGGTCCN AAGCCAGGTC CATATGTGTT CACTGGANCN NNANCACGGC
  841 TTGGGCTAAC CNGGGAACCC CCCCAGAAAC TNNGGNNTCN NGNANGAANN NTNNNNGNNN
901 GGGNNNTNNT CCNNNNNCNN NCCNNNCNNC ANNANCTNNN NNNNANNNNN NNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR
Sequence file: RDB18390_B0I7c.seq check
>08063_183903_B0I7_1_SV40pro_ori_F_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC TNTNNNNNNN NNNNNNNNNN
   61 GGCTTTTTTG GAGGCCTANG NTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC
  121 GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC
  181 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA
  241 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT
  301 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG
  361 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA
  421 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT
  481 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG
  541 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG
  601 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC
  661 GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGNNCCAT
  721 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC
  781 TGTGGCCGGC TGGGTGTGGC GGANCGCTAT CANGACATAG CGTTGGCTAC CCGNGATATT
  841 GCTGAAGANC TTGGCGGCGA ANGGGCTGAN CGCTTCCNCG NNCNTTACNG TATCGCCGCN
901 CCCNATTCNN ANCGCATCGC NNNCNATCGC NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919. [link to RRC of NBRP]
user_report Hamada, T., MALAT1 functions as a transcriptional promoter of MALAT1::GLI1 fusion for truncated GLI1 protein expression in cancer. BMC Cancer 23 (1): 424 (2023). PMID 37165307. [link to RRC of NBRP]
user_report Li, L., Upregulation of amplified in breast cancer 1 contributes to pancreatic ductal adenocarcinoma progression and vulnerability to blockage of hedgehog activation. Theranostics 11 (4): 1672-1689 (2021). PMID 33408774. [link to RRC of NBRP]
user_report Dettmer, R., FGF2 Inhibits Early Pancreatic Lineage Specification during Differentiation of Human Embryonic Stem Cells. Cells 9 (9): 1927 (2020). PMID 32825270. [link to RRC of NBRP]
user_report Mikami, Y., GLI-mediated Keratin 17 expression promotes tumor cell growth through the anti-apoptotic function in oral squamous cell carcinomas. J. Cancer Res. Clin. Oncol. 143 (8): 1381-1393 (2017). PMID 28342001. [link to RRC of NBRP]