RDB12186 - pcDNA3.1-pA83-Tead4VP16

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pcDNA3.1-pA83-Tead4VP16 (#RDB12186)

Expression clone of mouse Tead4, constitutive active

Clone info.	Expression clone of mouse Tead4, constitutive active. cDNA fragment 570 to 1,019 nt of NM_011567.2 (CDS, 570..1853) are cloned.
Comment	1-150aa of Tead4 fused to the activation domain of herpes simplex virus VP16. See Fig. 2B of Nishioka et al., Dev. Cell 16: 398-410 (2009). The expression vector has a stretch of poly(A). Known differences: Six histidine (6xHis)-, T7- and Xpress-tags are inserted upstream of the Tead4.
Vector backbone	pcDNA3.1-pA83 (Plasmid)
Size of vector backbone	5.4 kb
Selectable markers	Amp^r
Gene/insert name	Mouse Tead4 HSV VP16 protein cDNA
Depositor\|Developer	Sasaki, Hiroshi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12186	pcDNA3.1-pA83-Tead4VP16	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-pA83-Tead4VP16 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12186).

Reference section:

Nishioka, N., Inoue, K., Adachi, K., Kiyonari, H., Ota, M., Ralston, A., Yabuta, N., Hirahara, S., Stephenson, R.O., Ogonuki, N., Makita, R., Kurihara, H., Morin-Kensicki, E.M., Nojima, H., Rossant, J., Nakao, K., Niwa, H., Sasaki, H., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12186_A4Fup1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,T7 pro,6XHis,T7 tag,Xpress tag,insert 5'(Tead4_VP16) Sequence file: RDB12186_A4Fua.seq
>12186_12186_A4Fu_CMV-Foward_A08_02.ab1 1 AGTTCACTCC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA 61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG 121 ACTCACTATA GGGAGACCCA AGCTGGCTAG TTAAGCTTGG TACCGAGCTC GGATCCACTA 181 GTCCAGTGTG GTGGAATTGC CACCATGGGG GGTTCTCATC ATCATCATCA TCATGGTATG 241 GCTAGCATGA CTGGTGGACA GCAAATGGGT CGGGATCTGT ACGACGATGA CGATAAGGTA 301 CCAGGATCCA GTGTGGTGGA ATTCATTACC TCCAACGAGT GGAGCTCTCC CGACTCCCCC 361 GAGGGGAGCA GCATCTCTGG GGGCAGCCAG GCACTGGACA AGCCCATCGA CAATGATGCA 421 GAGGGTGTAT GGAGCCCCGA AATTGAGCGA AGCTTCCAGG AGGCCCTGGC CATCTACCCA 481 CCCTGCGGCC GCCGCAAAAT TATCCTGACG GAGGAAGGCA AGATGTATGG TCGGAATGAG 541 CTGATCGCAC GCCATATCAA GCTCAGGACA GGGAAGACGC GCACAAGGAA GCAGGTCTCC 601 AGCCACATCC AGGTGCTTGC CCGTCGAAAA GCCCGGGAGA TCCAGGCCAA ACTCAAGGAC 661 CAGGCAGCTA AGAACAAGGC CCTGCAGAGC ATGGCTGCCA TGTCGTCCGC ACAGATCGTC 721 TCGGCCACAG CCTTCCACAG TAAAATGGCT CTTGCCCGGG GCCCTGGCTA CCCAATCGAT 781 GCCCCCCCGA CCGATGTCAG CCTGGGGGAC GAGCTCCACT TAGACGGCGA GGACGTGGCG 841 ATGGCGCATG CCGACGCGCT AGACGATTTC GATCTGGACA TGTTGGGGGG ACGGGGGATT 901 CCCCGGGTCC GGGATTTACC CCCCCACGAC TCCGCCCCCT ACGGCGCTCT GGATATGGCC 961 GACTTCGAGT TTGAGCAGAT GTTTACCGAT GCCCTTGGGA AATTGACGAG TACGGTGGGT 1021 AGGGATATCC AGCACAGTGG CGGGCCCGCT CGACTATGGG CAACCAAAGA CCCTGTAACG 1081 TTTATCCTTT TTAATGGAAT GTGCACGAAT AAAGTTTGGC AAAGGCA //
Primer: GNP Primer #10 (Pr0763) Region: TRF2 3'-UTR and pA.insert (Tead4_VP16),Xpress tag,T7 tag,6XHis Sequence file: RDB12186_A4Fub.seq
>12186_12186_A4Fu_GNPprimer#10_E02_14.ab1 1 TTAATTTGTG CAATTCATTT AAAAGATAAC GTTTACAGGT TCTTTGGTGC CCATAGTCGA 61 GCGGCCGCCA CTGTGCTGGA TATCCTACCC ACCGTACTCG TCAATTCCAA GGGCATCGGT 121 AAACATCTGC TCAAACTCGA AGTCGGCCAT ATCCAGAGCG CCGTAGGGGG CGGAGTCGTG 181 GGGGGTAAAT CCCGGACCCG GGGAATCCCC GTCCCCCAAC ATGTCCAGAT CGAAATCGTC 241 TAGCGCGTCG GCATGCGCCA TCGCCACGTC CTCGCCGTCT AAGTGGAGCT CGTCCCCCAG 301 GCTGACATCG GTCGGGGGGG CATCGATTGG GTAGCCAGGG CCCCGGGCAA GAGCCATTTT 361 ACTGTGGAAG GCTGTGGCCG AGACGATCTG TGCGGACGAC ATGGCAGCCA TGCTCTGCAG 421 GGCCTTGTTC TTAGCTGCCT GGTCCTTGAG TTTGGCCTGG ATCTCCCGGG CTTTTCGACG 481 GGCAAGCACC TGGATGTGGC TGGAGACCTG CTTCCTTGTG CGCGTCTTCC CTGTCCTGAG 541 CTTGATATGG CGTGCGATCA GCTCATTCCG ACCATACATC TTGCCTTCCT CCGTCAGGAT 601 AATTTTGCGG CGGCCGCAGG GTGGGTAGAT GGCCAGGGCC TCCTGGAAGC TTCGCTCAAT 661 TTCGGGGCTC CATACACCCT CTGCATCATT GTCGATGGGC TTGTCCAGTG CCTGGCTGCC 721 CCCAGAGATG CTGCTCCCCT CGGGGGAGTC GGGAGAGCTC CACTCGTTGG AGGTAATGAA 781 TTCCACCACA CTGGATCCTG GTACCTTATC GTCATCGTCG TACAGATCCC GACCCATTTG 841 CTGTCCACCA GTCATGCTAG CCATACCATG ATGATGATGA TGATGAGAAC CCCCCATGGT 901 GGCAATTCCA CCACACTGGA CTAGTGGATC CGAGCTCGGT ACCAAGCTTT AACTAGCCAG 961 CTTGGGTCTC CCTATAGTGA GTCGTATTAA TTTCGATAAG CCAGTAAGCA GTGGTCTCTA 1021 GTTAGCCCAG AGAGCTCTGC TTATATAGAC CTCCCCACCG TACACCGCCT ACGCCATTGC 1081 GTCAATGGGG GCGGAGTGTA CGACATTTGA AGGTCGATTG ATTGGTTCAA CATTCCATGA 1141 CGCTTCCAAT G //
Primer: BGH_rev2 (Pr0606) Region: bGHpA,TRF2 3'-UTR and pA Sequence file: RDB12186_A4Fuc.seq
>12186_12186_A4Fu_BGH_rev2_F02_17.ab1 1 GTAGACTGAG GGGTTAAGGG GGGGCACGGG GGAGGGGCAA ACAACAGATG GCTGGCAACT 61 AGAAGGCACA GTCGAGGCTG ATCAGCGGGT TTAAACTCAA TGGTGATGGT GATGATGACC 121 GGTATGCATA TTCAGATCCT CTTCTGAGAT GAGTTTTTGT TCGAAGGGCC CTCTAGACTC 181 GAGGTACGCC ACAGCGTATG ATGCGTATTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT 241 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TCTTTCCCCA AATTTTTTTT 301 TGGCCCCTTC AATTTAAAAA AAAAAATTTT TCCGGTTTTT TTGGGCCCCA ATACCAACCC 361 CCCCCCCGGG GGGGGAATTT CCCCCCCCCG GACCCCCTAT TTTCAGGGGG GCGGGGAAAA 421 CTTTCCCCAC AACCCAAAAT GCGCCAATTC CAAAACCCCC TTGGGGGGGG GAACCCGGGG 481 GGGGAAAATC CCGGGACCGG GGGAAAACCC CCCCCCCCCA ATTTCAAAAA AAAAAATTTT 541 AAGGGGGGGG GGGGCCCCCC CCCCCCCCCC CCCCCTAAAA GGAGACCCCC CCCCCCCATT 601 GAAAACGGGG GGGGGGCCCA ATTGGTAACC CGGGCCCCCC GGCAAAAAAC CTTTTTTTTG 661 GAAAGGGGGG GGGAAAAAAA AATTTTGGGA AAAAATGAAC CCTTCCTTTG GGGGGCTTTT 721 TTTATCCCCC GGCCTTGATT GGGGGATATC CCGGGTTTTT TAGGGGAAAC CCTGAGGTTG 781 GGTGTGAGAG CCTTTTTTTG GGCGCCTCTC CTCTGCCGCG AGAGTATGGG GGGGGAGACT 841 CTCTTCCACA CAACTTTTCT CTTCTCCCCG AGGATATATT TTGCCCGCCG CGCGGGGGAA 901 TAGACGCGCC TCGGAAACAT CTCTTTGTGG GGGCTCCCAC CCCTACTTTA TGAGAGAGTT 961 CACGACGGCG CCACACAATA CTCTCTCCTC TGCGGGAGCG AGAACCACCC TCGACGGGTA 1021 GTGATACTAC ACGCGAGATG ATGGTGCTC //
Primer: SV40pro_F (Pr0110) Region: SV40 pro_ori,NeoR Sequence file: RDB12186_A4Fud.seq
>12186_12186_A4Fu_SV40pro_F_H02_23.ab1 1 CCCGGGTCCC TTTAACATTC AGGAATAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG 61 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG 121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA 181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT 241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT 301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG 361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT 421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC 481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC 541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA 601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG 661 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT 721 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG 781 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGGC GGCGAATGGG 841 CTGACCGCTT CCTCGTGCTT TACGGGTATC GCCGCTTCCG ATTCGCAGCG CATCGCCCTT 901 CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGGTTCGCGA AATGACCGAA 961 CCAAGCGACG CCCCAACCTG CCATCACGAG GATTTCCGAA TTCCACCGGC CGCCCTTCTT 1021 ATGAAACGGT TGGGGCTTCG GAATCGGTTT CAGAACGTCC GGGCTGGGAA TGAATCTTCA 1081 AGCGCGGGGA TCTCAATGGC TGGAGTCCTT CGCCAACCCC AACCTTGGTT TATCTGGCAG 1141 ACCCT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. [link to RRC of NBRP]

Resource search

Link

Other resources in our bank

mouse Tead4

External Database
Mouse Tead4

HSV VP16 protein

Reference sequence