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pcDNA3.1-pA83-Tead1VP16 (#RDB12188)

Expression clone of mouse Tead1, constitutive active

Clone info. Expression clone of mouse Tead1, constitutive active.
Comment 1-151aa of Tead1 (cDNA fragment corresponding to 426 nt to 879 nt of NM_001166585.2) fused to the activation domain of herpes simplex virus VP16. See Fig. 2B of Nishioka et al., Dev. Cell 16: 398-410 (2009). The expression vector has a stretch of poly(A).
Known difference : T to G substitution (Met, synonym) at 428nt of NM_001166585.2.
Vector backbone pcDNA3.1-pA83 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Mouse Tead1 cDNA HSV VP16 protein gene cDNA
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12188 pcDNA3.1-pA83-Tead1VP16 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-pA83-Tead1VP16 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12188).

Reference section:

Nishioka, N., Inoue, K., Adachi, K., Kiyonari, H., Ota, M., Ralston, A., Yabuta, N., Hirahara, S., Stephenson, R.O., Ogonuki, N., Makita, R., Kurihara, H., Morin-Kensicki, E.M., Nojima, H., Rossant, J., Nakao, K., Niwa, H., Sasaki, H., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12188_A6A7p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,FLAG,Myc,insert 5'(Tead1)
Sequence file: RDB12188_A6A7e.seq check
>12188_12188_A6A7_CMV-Forward_B11_05.ab1
    1 NNNNNNNNNN NNNNNTNNNN GCNNTGGGCG GTNNGCGTGT NCGGNGGGNN GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAANCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGGCTAGT TAAGCTTGGT ACCGAGCTCG GATCCACTAG
  181 TCCAGTGTGG TGGAATTAAA TTCCACCATG GACTACAAGG ACGACGATGA CAAGGGATCC
  241 GAATTCGAGC TCCGTCGAAT GGAACAGAAA CTGATTAGCG AAGAAGATCT GAACGAAGGC
  301 CGCATGGAGC CCAGCAGCTG GAGCGGCAGC GAGAGCCCTG CCGAAAACAT GGAAAGGATG
  361 AGCGACTCGG CAGATAAGCC GATTGACAAC GACGCGGAGG GCGTCTGGAG TCCTGATATT
  421 GAGCAGAGTT TCCAGGAGGC CCTGGCTATC TATCCGCCGT GTGGGAGGAG AAAAATCATC
  481 TTATCAGACG AAGGCAAAAT GTATGGTAGA AATGAATTGA TAGCCAGATA CATCAAACTC
  541 AGGACGGGAA AGACAAGGAC CAGGAAGCAG GTGTCTAGTC ACATTCAGGT TCTTGCCAGA
  601 AGGAAATCTC GTGNTTTTCA TTCCAAGCTG AANGATCAGA CTGCCAAGGA CAAGGCCCTG
  661 CAGCACATGG CTGCCATGTC ATCAGCCCAG ATCGTCTCGG CTACTGCCAT CCACAACAAG
  721 CTGNNGCTGC CTNNNNNTCC ACGCCCCACC TTCCCGGCCC CCCCNANCGA TGTCAGCCTG
  781 GGGGANGANC TCCACTTANA CGGCNNNGNN GTGGCGATGG CGCATGCCNN NNCNCNANNC
  841 GANTTNNNTC TNNNNATNNT NGGGGNNNGG GNNNTNCNNN GNNNNNGNNN TTNCNCCCNN
901 NNNNNNNN
//
Primer: GNP Primer #10 (Pr0763)
Region: TRF2-3'UTR and pA,insert 3'(VP16,Tead1)
Sequence file: RDB12188_A6A7f.seq check
>12188_12188_A6A7_GNP_primer#10_C11_08.ab1
    1 NNNNNNNNNN NNNATTTAAN NGNNAACGTT TACNGGTTCT TTGGTGCCCA TAGTCGAGCG
   61 GCCGCCACTG TGCTGGATAT CTGCAGAATT CTACCCACCG TACTCGTCAA TTCCAAGGGC
  121 ATCGGTAAAC ATCTGCTCAA ACTCGAAGTC GGCCATATCC AGAGCGCCGT AGGGGGCGGA
  181 GTCGTGGGGG GTAAATCCCG GACCCGGGGA ATCCCCGTCC CCCAACATGT CCAGATCGAA
  241 ATCGTCTAGC GCGTCGGCAT GCGCCATCGC CACGTCCTCG CCGTCTAAGT GGAGCTCGTC
  301 CCCCAGGCTG ACATCGGTCG GGGGGGCCGG GAAGGTGGGG CGTGGAATCC CAGGCAGCCC
  361 CAGCTTGTTG TGGATGGCAG TAGCCGAGAC GATCTGGGCT GATGACATGG CAGCCATGTG
  421 CTGCAGGGCC TTGTCCTTGG CAGTCTGATC CTTCAGCTTG GAATGAAAAT CACGAGATTT
  481 CCTTCTGGCA AGAACCTGAA TGTGACTAGA CACCTGCTTC CTGGTCCTTG TCTTTCCCGT
  541 CCTGAGTTTG ATGTATCTGG CTATCAATTC ATTTCTACCA TACATTTTGC CTTCGTCTGA
  601 TAAGATGATT TTTCTCCTCC CACACGGCGG ATAGATAGCC AGGGCCTCCT GGAAACTCTG
  661 CTCAATATCA GGACTCCAGA CGCCCTCCGC GTCGTTGTCA ATCGGCTTAT CTGCCGANTC
  721 GCTCATCCTT TCCATGTTTT CGGCAGGGCT CTCGCTGCCG CTCCAGCTGC TGGGCTCCAT
  781 GCGGCCTTCG TNCAGATCTT CTTCGCNNAT CAGTTTCTGT NNNNTTCGAC GGNNNTCNAN
  841 NNCNNNNNCC TNNNCATCGN CGNCCTNGNA NNNCANNGNN NNATTTNNNT CCNCNNCACN
901 NGNNCTANNN GNNTCCNNNN NNNNNACNNN NNNNTNNNNN NNNNN
//
Primer: bGH_rev3 (Pr0885)
Region: bGHpA,TRF2-3'UTR and pA
Sequence file: RDB12188_A6A7g.seq check
>12188_12188_B0JlS_bGH_rev3_E04_13_ABI24.ab1
    1 NNNNNNNNNN NNNNGNNGGG CAACAACAGA TGGCTGGCAA CTAGAAGGCA CAGTCGAGGC
   61 TGATCAGCGG GTTTAAACTC AATGGTGATG GTGATGATGA CCGGTATGCA TATTCAGATC
  121 CTCTTCTGAG ATGAGTTTTT GTTCGAAGGG CCCTCTAGAC TCGAGGTACG CCACAGCGTA
  181 TGATGCGTAT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
241 NNNNNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: NeoR
Sequence file: RDB12188_A6A7h.seq check
>12188_12188_A6A7_SV40pro_F_D11_11.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNTGNNG NNGCTTTTTT GGNNGCCTAN GCTTTTGCAA
   61 AAAGCTCCCG GGANCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GNTGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GANGNNCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA
  781 TCANGACATA GCGTTGGCTA CCCGNNNNAT TGCTGAAGAN CTTGGCGGCG AANGGGCTGA
  841 NCGCTNCCTC NNGCTTTACN GTATCGCCGC NNCCNNNTCN CANCGCNTCG CCNNCTATCN
901 NNNTNNNNNC NNNTNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. [link to RRC of NBRP]