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pcDNA3.1-pA83-Tead1VP16

Expression clone of mouse Tead1, constitutive active

Catalog number RDB12188
Resource name pcDNA3.1-pA83-Tead1VP16
Clone info. Expression clone of mouse Tead1, constitutive active.
1-151aa of Tead1 fused to the activation domain of herpes simplex virus VP16. See Fig. 2B of Nishioka et al., Dev. Cell 16: 398-410 (2009). The expression vector has a stretch of poly(A).
Vector backbone pcDNA3.1-pA83 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Mouse Tead1 cDNA HSV VP16 protein gene cDNA
Depositor Sasaki, Hiroshi |
 
Other clones in our bank mouse Tead1 (NCBI Gene 21676) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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RDB12188 pcDNA3.1-pA83-Tead1VP16 DNA solution

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References and tips

Electronic file

Featured content

References

original Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12188_A6A7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB12188_A6A7a.seq
>12188_12188_A6A7_CMV-Forward_B11_05.ab1
    1 CTTTCACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGGCTAGT TAAGCTTGGT ACCGAGCTCG GATCCACTAG
  181 TCCAGTGTGG TGGAATTAAA TTCCACCATG GACTACAAGG ACGACGATGA CAAGGGATCC
  241 GAATTCGAGC TCCGTCGAAT GGAACAGAAA CTGATTAGCG AAGAAGATCT GAACGAAGGC
  301 CGCATGGAGC CCAGCAGCTG GAGCGGCAGC GAGAGCCCTG CCGAAAACAT GGAAAGGATG
  361 AGCGACTCGG CAGATAAGCC GATTGACAAC GACGCGGAGG GCGTCTGGAG TCCTGATATT
  421 GAGCAGAGTT TCCAGGAGGC CCTGGCTATC TATCCGCCGT GTGGGAGGAG AAAAATCATC
  481 TTATCAGACG AAGGCAAAAT GTATGGTAGA AATGAATTGA TAGCCAGATA CATCAAACTC
  541 AGGACGGGAA AGACAAGGAC CAGGAAGCAG GTGTCTAGTC ACATTCAGGT TCTTGCCAGA
  601 AGGAAATCTC GTGATTTTCA TTCCAAGCTG AAGGATCAGA CTGCCAAGGA CAAGGCCCTG
  661 CAGCACATGG CTGCCATGTC ATCAGCCCAG ATCGTCTCGG CTACTGCCAT CCACAACAAG
  721 CTGGGGCTGC CTGGGATTCC ACGCCCCACC TTCCCGGCCC CCCCGACCGA TGTCAGCCTG
  781 GGGGACGAGC TCCACTTAGA CGGCGAGGAC GTGGCGATGG CGCATGCCGA CGCGCTAGAC
  841 GATTTCGATC TGGACATGTT GGGGGGACGG GGATTCCCCG GGTCCGGGAT TTACCCCCCA
  901 CGACTCCGCC CCCCTACGGC GCTCTGGGAT ATGGGCCGAC TTTCGAGTTT GAGCAGATGT
  961 TTACCCGAAT GGCCGTGGTA GTGAACGAGT ACGTGGTAGG ATCTGCCAGA ATATCAGCAC
1021 AGTGACGACG GCTGACTATG GCACGGAGAG CTGTAAGCGA TT
//
Primer: GNP_primer#10 RDB12188_A6A7b.seq
>12188_12188_A6A7_GNP_primer#10_C11_08.ab1
    1 CTACGTGCAA TTCATTTAAA AGATAACGTT TACAGGTTCT TTGGTGCCCA TAGTCGAGCG
   61 GCCGCCACTG TGCTGGATAT CTGCAGAATT CTACCCACCG TACTCGTCAA TTCCAAGGGC
  121 ATCGGTAAAC ATCTGCTCAA ACTCGAAGTC GGCCATATCC AGAGCGCCGT AGGGGGCGGA
  181 GTCGTGGGGG GTAAATCCCG GACCCGGGGA ATCCCCGTCC CCCAACATGT CCAGATCGAA
  241 ATCGTCTAGC GCGTCGGCAT GCGCCATCGC CACGTCCTCG CCGTCTAAGT GGAGCTCGTC
  301 CCCCAGGCTG ACATCGGTCG GGGGGGCCGG GAAGGTGGGG CGTGGAATCC CAGGCAGCCC
  361 CAGCTTGTTG TGGATGGCAG TAGCCGAGAC GATCTGGGCT GATGACATGG CAGCCATGTG
  421 CTGCAGGGCC TTGTCCTTGG CAGTCTGATC CTTCAGCTTG GAATGAAAAT CACGAGATTT
  481 CCTTCTGGCA AGAACCTGAA TGTGACTAGA CACCTGCTTC CTGGTCCTTG TCTTTCCCGT
  541 CCTGAGTTTG ATGTATCTGG CTATCAATTC ATTTCTACCA TACATTTTGC CTTCGTCTGA
  601 TAAGATGATT TTTCTCCTCC CACACGGCGG ATAGATAGCC AGGGCCTCCT GGAAACTCTG
  661 CTCAATATCA GGACTCCAGA CGCCCTCCGC GTCGTTGTCA ATCGGCTTAT CTGCCGAGTC
  721 GCTCATCCTT TCCATGTTTT CGGCAGGGCT CTCGCTGCCG CTCCAGCTGC TGGGCTCCAT
  781 GCGGCCTTCG TTCAGATCTT CTTCGCTAAT CAGTTTCTGT TCCATTCGAC GGAGCTCGAA
  841 TTCGGATCCC TTGTCATCGT CGTCCTTGTA GTCCATGGTG GAATTTAATT CCACCACACT
  901 GGGACTAGTG GGATCCGAGC TCGGTACCAA AGCTTTAACT AGCCAGCTTT GGGGTCTCCC
  961 CTATAGTGAG TCGTATTAAA TTTCGATAAG CCAGTAAGCA GTGGGGTTCT TCTAGTTTAG
 1021 CCAGAGAGCT CCTGCCTTAT ATAGACCCTC CCACCGTACA CGTCCTTAAC CGACCCATTT
1081 GGGCGTTCAA ATTGG
//
Primer: SV40pro_F RDB12188_A6A7c.seq
>12188_12188_A6A7_SV40pro_F_D11_11.ab1
    1 CTTGGTCCTG TGACATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GCGAAATGAC CGACCCAAGC
  961 GACGCCCAAC CCTGCCCATC ACGAGATTTC GATTTCCCAC CCGCCCGGCC GTTCCTATGA
 1021 AAGGATGGGC ATCCGGCATC GTTTTCTGGG ACGCTGACTG CATGAATCCT CCAGCGACGG
1081 GGGTAATCCT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085.

2019.04.09

GNP_filter3_RDBDEP_html_190327.pl