Please review the QC test results indicated by icon below as well as clone information before placing your order.

pcDNA3.1-pA83-Tead1VP16

Expression clone of mouse Tead1, constitutive active

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12188_A6A7p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro,FLAG,Myc,insert 5'(Tead1) Sequence file: RDB12188_A6A7e.seq
>12188_12188_A6A7_CMV-Forward_B11_05.ab1     1 NNNNNNNNNN NNNNNTNNNN GCNNTGGGCG GTNNGCGTGT NCGGNGGGNN GTCTATATAA    61 GCAGAGCTCT CTGGCTAACT AGAGAANCCA CTGCTTACTG GCTTATCGAA ATTAATACGA   121 CTCACTATAG GGAGACCCAA GCTGGCTAGT TAAGCTTGGT ACCGAGCTCG GATCCACTAG   181 TCCAGTGTGG TGGAATTAAA TTCCACCATG GACTACAAGG ACGACGATGA CAAGGGATCC   241 GAATTCGAGC TCCGTCGAAT GGAACAGAAA CTGATTAGCG AAGAAGATCT GAACGAAGGC   301 CGCATGGAGC CCAGCAGCTG GAGCGGCAGC GAGAGCCCTG CCGAAAACAT GGAAAGGATG   361 AGCGACTCGG CAGATAAGCC GATTGACAAC GACGCGGAGG GCGTCTGGAG TCCTGATATT   421 GAGCAGAGTT TCCAGGAGGC CCTGGCTATC TATCCGCCGT GTGGGAGGAG AAAAATCATC   481 TTATCAGACG AAGGCAAAAT GTATGGTAGA AATGAATTGA TAGCCAGATA CATCAAACTC   541 AGGACGGGAA AGACAAGGAC CAGGAAGCAG GTGTCTAGTC ACATTCAGGT TCTTGCCAGA   601 AGGAAATCTC GTGNTTTTCA TTCCAAGCTG AANGATCAGA CTGCCAAGGA CAAGGCCCTG   661 CAGCACATGG CTGCCATGTC ATCAGCCCAG ATCGTCTCGG CTACTGCCAT CCACAACAAG   721 CTGNNGCTGC CTNNNNNTCC ACGCCCCACC TTCCCGGCCC CCCCNANCGA TGTCAGCCTG   781 GGGGANGANC TCCACTTANA CGGCNNNGNN GTGGCGATGG CGCATGCCNN NNCNCNANNC   841 GANTTNNNTC TNNNNATNNT NGGGGNNNGG GNNNTNCNNN GNNNNNGNNN TTNCNCCCNN 901 NNNNNNNN //
Primer: GNP Primer #10 Region: TRF2-3'UTR and pA,insert 3'(VP16,Tead1) Sequence file: RDB12188_A6A7f.seq
>12188_12188_A6A7_GNP_primer#10_C11_08.ab1     1 NNNNNNNNNN NNNATTTAAN NGNNAACGTT TACNGGTTCT TTGGTGCCCA TAGTCGAGCG    61 GCCGCCACTG TGCTGGATAT CTGCAGAATT CTACCCACCG TACTCGTCAA TTCCAAGGGC   121 ATCGGTAAAC ATCTGCTCAA ACTCGAAGTC GGCCATATCC AGAGCGCCGT AGGGGGCGGA   181 GTCGTGGGGG GTAAATCCCG GACCCGGGGA ATCCCCGTCC CCCAACATGT CCAGATCGAA   241 ATCGTCTAGC GCGTCGGCAT GCGCCATCGC CACGTCCTCG CCGTCTAAGT GGAGCTCGTC   301 CCCCAGGCTG ACATCGGTCG GGGGGGCCGG GAAGGTGGGG CGTGGAATCC CAGGCAGCCC   361 CAGCTTGTTG TGGATGGCAG TAGCCGAGAC GATCTGGGCT GATGACATGG CAGCCATGTG   421 CTGCAGGGCC TTGTCCTTGG CAGTCTGATC CTTCAGCTTG GAATGAAAAT CACGAGATTT   481 CCTTCTGGCA AGAACCTGAA TGTGACTAGA CACCTGCTTC CTGGTCCTTG TCTTTCCCGT   541 CCTGAGTTTG ATGTATCTGG CTATCAATTC ATTTCTACCA TACATTTTGC CTTCGTCTGA   601 TAAGATGATT TTTCTCCTCC CACACGGCGG ATAGATAGCC AGGGCCTCCT GGAAACTCTG   661 CTCAATATCA GGACTCCAGA CGCCCTCCGC GTCGTTGTCA ATCGGCTTAT CTGCCGANTC   721 GCTCATCCTT TCCATGTTTT CGGCAGGGCT CTCGCTGCCG CTCCAGCTGC TGGGCTCCAT   781 GCGGCCTTCG TNCAGATCTT CTTCGCNNAT CAGTTTCTGT NNNNTTCGAC GGNNNTCNAN   841 NNCNNNNNCC TNNNCATCGN CGNCCTNGNA NNNCANNGNN NNATTTNNNT CCNCNNCACN 901 NGNNCTANNN GNNTCCNNNN NNNNNACNNN NNNNTNNNNN NNNNN //
Primer: bGH_rev3 Region: bGHpA,TRF2-3'UTR and pA Sequence file: RDB12188_A6A7g.seq
>12188_12188_B0JlS_bGH_rev3_E04_13_ABI24.ab1     1 NNNNNNNNNN NNNNGNNGGG CAACAACAGA TGGCTGGCAA CTAGAAGGCA CAGTCGAGGC    61 TGATCAGCGG GTTTAAACTC AATGGTGATG GTGATGATGA CCGGTATGCA TATTCAGATC   121 CTCTTCTGAG ATGAGTTTTT GTTCGAAGGG CCCTCTAGAC TCGAGGTACG CCACAGCGTA   181 TGATGCGTAT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT 241 NNNNNNNNNN //
Primer: SV40pro_ori_F Region: NeoR Sequence file: RDB12188_A6A7h.seq
>12188_12188_A6A7_SV40pro_F_D11_11.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNTGNNG NNGCTTTTTT GGNNGCCTAN GCTTTTGCAA    61 AAAGCTCCCG GGANCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GNTGAGGATC   121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG   181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG   241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA   301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC   361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC   421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA   481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA   541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT   601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT   661 GCCCGACGGC GANGNNCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT   721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA   781 TCANGACATA GCGTTGGCTA CCCGNNNNAT TGCTGAAGAN CTTGGCGGCG AANGGGCTGA   841 NCGCTNCCTC NNGCTTTACN GTATCGCCGC NNCCNNNTCN CANCGCNTCG CCNNCTATCN 901 NNNTNNNNNC NNNTNNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl