Resource data sheet
Prev.

pMYs-HA-Yap1-IRES-EGFP

Retroviral vector of mouse Yap1

Catalog number RDB12177
Resource name pMYs-HA-Yap1-IRES-EGFP
Clone info. Retroviral vector of mouse Yap1. cDNA fragment 210 to 1,625 nt of NM_009534.3 (CDS, 207..1625) is cloned.
Transfection can be confirmed by GFP. See Fig. 3A of Ota and Sasaki, Development 135: 4059-4069 (2008). Known differences: G to C substitution at 212 nt (Glu to Asp at 2nd aa). C to A substitution at 477 nt (Arg at 91st aa, synonymous) and XhoI site insertion at the position.
Vector backbone pMYs-IRES-EGFP (Plasmid)
Size of vector backbone 6.3 kb
Selectable markers Amp^r
Gene/insert name Mouse Yap1 cDNA
Depositor Sasaki, Hiroshi |
 
Other clones in our bank mouse Yap1 (NCBI Gene 22601) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Additional terms and conditions for distribution Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
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Schedule A (GFP-A) [Link]
Remarks Remember that you will be working with samples containing infections virus.
Please use Schedule A (GFP-A) additionally.
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RDB12177 pMYs-HA-Yap1-IRES-EGFP DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12177_A6Ajp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: 5'Retro (pFB-5') RDB12177_A6Aja.seq
>12177_12177_A6Aj_5'Retro(pFB-5')_C09_09.ab1
    1 GAGCGGGAGG GAATTCTTAT CGGACTGCTG TTTATTAGGA TCCCAGTGTG GTGGTACGGG
   61 AATTCCTGCA GGGGGCTAGC GAATTCGATT AATACGACTC ACTATAGGGA GACCCAAGCT
  121 TGCCGCCACC ATGTACCCAT ACGACGTTCC AGATTACGCT GGTACCGACC CCGCGCAACA
  181 GCCGCCGCCC CAGCCGGCCC CGCAAGGCCC CGCGCCGCCG TCCGTGTCTC CGGCCGGGAC
  241 CCCCGCGGCC CCGCCCGCAC CCCCGGCCGG CCACCAGGTC GTGCACGTCC GCGGGGACTC
  301 GGAGACCGAC TTGGAGGCGC TCTTCAATGC CGTCATGAAC CCCAAGACGG CCAACGTGCC
  361 TCAGACCGTG CCCATGCGGC TTCGCAAGCT GCCCGACTCC TTCTTCAAGC CGCCTGAGCC
  421 CAAGTCCCAC TCGAGACAGG CCAGTACTGA TGCAGGTACT GCGGGAGCTC TGACTCCACA
  481 GCATGTTCGA GCTCACTCCT CTCCAGCCTC CCTGCAGCTG GGTGCCGTTT CTCCTGGGAC
  541 ACTCACAGCC AGTGGCGTTG TCTCTGGCCC TGCCGCTGCC CCTGCAGCTC AGCATCTCCG
  601 GCAGTCCTCC TTTGAGATCC CTGATGATGT ACCACTGCCA GCAGGCTGGG AGATGGCCAA
  661 GACATCTTCT GGTCAAAGAT ACTTCTTAAA TCACAACGAT CAGACAACAA CATGGCAGGA
  721 CCCCCGGAAG GCCATGCTTT CGCAACTGAA CGTTCCTGCG CCTGCCAGCC CAGCGGTGCC
  781 CCAGACGCTG ATGAATTCTG CCTCAGGACC TCTTCCTGAT GGATGGGAGC AAGCCATGAC
  841 TCAGGATGGA GAAGTTTACT ACATAAACCA TAAGAACAAG ACCACATCCT GGCTGGACCC
  901 AAGGGCTGGA CCCTCGTTTT GCCATGAACC AGAGGATCAC TCAGAGTGCT CCAGTGAAGC
  961 AGCCCCCACC CTTGGCTCCC CCAGAGCCCA CAGGGAGGCG TTCCTGGGTG GAGGCAGTTT
 1021 CCAAACCAGC AGCAGCAAAT ACAGCTGCAG CAGTTACAGA TGGAGAGGAG AGACTGCGGT
 1081 TGAAACAACA GGATTATTTT CGGCAGATTT AGCTCTTGCG CAGCCAGTTG GCTTACACTG
1141 GGAGCCAAGG AATGGAAGGG AAACT
//
Primer: IRES-R RDB12177_A6Ajb.seq
>12177_12177_A6Aj_IRES-R_B09_06.ab1
    1 GCAACGGGCG GCTTCGGCCA GTACGTTAGG GGGGGGGGAG GGAGAGGGGG GGGGGGGGCG
   61 GAATTTACGT AGCGGCCGCG GCGCGCCGGC CCTCGAGGAT CTGCAGAATT CCAGCACACT
  121 GGCGGCCGTT ACTAGTGGAT CCCTCACAGA CTCAGAGTGG CTCCCTGCAG CTCTATAACC
  181 ACGTGAGAAA GCTTTCTTTA TCTAGCTTGG TGGCAGCCAA CACAGACTCC ACGTCCAAGA
  241 TTTCGGAACT CAGCGCTTCC TGCAGACTGG GCATCAGCTC CTCCCCTTCT ATGTTCATTG
  301 CATCTCCTTC CAGTGTGCCA AGGTCCACAT TTGTCCCAGG GAGGGCTTCC AGGTAGTCGG
  361 GGAAGCGGCT CTGCTGTGAC GGCAGGGTGC TTTGGCTGAT GGTGTCTCCT GTATCCATTT
  421 CATCCACACT GTTGAGGAAG TCGTCTGGGG TCCGAGGGAT GCTGTAGCTG CTCATGCTGA
  481 GGCCGCTGTC TGTGCTCTCA TCTCGAGAGT GATAGGTGCC ACTGTTAAGA AAGGGATCGG
  541 AACTATTGGT TGTCATTGTT CTCAATTCCT GAGACATCCC AGGAGAAGAC ACTGCATTCG
  601 GAGTCCCTCC ATCCTGCTCC AGTGTAGGCA ACTGGCTGCG CAGAGCTAAT TCCTGCCGAA
  661 ATAATTCCTG TTGTTTCAAC CGCAGTCTCT CCTTCTCCAT CTGTAACTGC TGCAGCTGTA
  721 TTTGCTGCTG CTGGTTGGAA CTGCCTCCAC CCAGGACGCC TCCCTGTGGG CTCTGGGGAG
  781 CCAAGGGTGG GGGCTGCTTC ACTGAAGCAC TCTGAGTGAT CCTCTGGTTC ATGGGCAAAA
  841 CGAGGGTCCA GCCTTGGGGT CCAGCCAGGA TGTGGTCTTT GTTCTTTATG GTTTATGTAG
  901 TAAACTTCTC CATCCTGAAG TCGTGGGCTT TGCTCCCATC CATCAGGAAA AAGGTCCTGA
  961 AGGCAAAATT CATCACCGTT CTGGGGGCAA CCGCTGGGCT GGCAGGCGCA AGAACCGTTT
1021 CCATTTGCGA AAGCATTGGC CCTTTCCGGG GGG
//
Primer: IRES-F RDB12177_A6Ajc.seq
>12177_12177_A6Aj_IRES-F_D09_12.ab1
    1 GCATCGTTAT GTGTTTAGTC GAGGTTAAAA ACGTCTAGGC CCCCCGAACC ACGGGGACGT
   61 GGTTTTCCTT TGAAAAACAC GATGATAATA TGGCCACAAC CATGGTGAGC AAGGGCGAGG
  121 AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA
  181 AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT
  241 TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT
  301 ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT
  361 CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT
  421 ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA
  481 AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA
  541 ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA
  601 AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA
  661 CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG
  721 CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG
  781 CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTA AAGCGGCCGG CCGCCAGCAC
  841 AGTGGTCGAC CGGGCCGCCA GCACAGTGGT CGATCGAGGG GGGGCCCGCG ATTAGTCCAA
  901 TTTGTTAAAG ACAGGATATC AGTGGTCCAG GCTCTAGTTT TGACTCAACA ATATCACCAG
  961 CTGAAGCCTA TAGAGTACGA GCCATAGATA GAATAAAAGA TTTTATTTAG TCTCCAGAAA
 1021 AATGGGGGGA AATGAAAGAC CCCACCTGTA GTTTTGGCAA GCTAGCCTCA GTACGCATTT
1081 TGCAGGCATG CAAATACGTA CCTGAGATAG AGTAGTTCAG ATCAAGGGTT AGGACCG
//
Primer: Amp_R RDB12177_A6Ajd.seq
>12177_12177_A6Aj_Amp_R_E09_15.ab1
    1 CTCTTTTCCA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
   61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
  121 ACCTGCATAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT
  181 TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAAAAGT TCAGATCAAG GTCAGGAACA
  241 GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC
  301 TCAGGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC
  361 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG
  421 CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT
  481 GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC
  541 CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATTGACTGA
  601 GTCGCCCGGG TACCCGTATT CCCAATAAAG CCTCTTGCTG TTTGCATCCG AATCGTGGAC
  661 TCGCTGATCC TTGGGAGGGT CTCCTCAGAT TGATTGACTG CCCACCTCGG GGGTCTTTCA
  721 TTTGGAGGTT CCACCGAGAT TTGGAGACCC CTGCCCAGGG ACCACCGACC CCCCCGCCGG
  781 GAGGTAAGCT GGCCAGCGGT CGTTTCGTGT CTGTCTCTGT CTTTGTGCGT GTTTGTGCCG
  841 GCATCTAATG TTTGCGCCTG CGTCTGTACT AGTTTAGCTA ACTAGCTCTG TATCTGGCGG
  901 ACCCGTGGTG GAACTGACGA GTTCGGAACA CCCGGGCCGC AACCCTGGGA GACGTCCCAG
  961 GGACTTCGGG GGGCCGTTTT TGTGGCCCGA CCTGAGTCCA AAAAATCCCG ATCGTTTTGG
 1021 ACTCTTTGTT GCACCCCCCC CTAATAAGAG GGATTATTGT GGATTTCTTG GGTAGGAAGA
 1081 CGAGACTTAA AACAGGTTCC CGCCTTTCGT CTGATTTTTG GCTTTCGGTT TTGGGACCGA
1141 AGCCGCGCCC GAGCCGCATC CTTTGGAC
//
Primer: pMFG_1 RDB12177_A6Aje.seq
>12177_12177_A6Aj_pMFG-1_F09_18.ab1
    1 GACATGGGGA GAGGGGCTGT TAGTAACTCT TGTCATGTCA GGGTCAGGGA AGTTTACAAG
   61 GGGCGGGGGT GCCCCATATA AGATCTCATA TGGCCATATG GGGGCGCCTA GAGAAGGAGT
  121 GAGGGCTGGA TAAAGGGAGG ATCGAGGCGG GGTCGAACGA GGAGGTTCAA GGGGGAGAGA
  181 CGGGGCGGAT GGAGGAAGAG GAGGCGGAGG CTTAGGGTGT ACAAAGGGCT TGACCCAGGG
  241 AGGGGGGTCA AAAGCCAAGG CTTCCCAGGT CACGATGTAG GGGACCTGGT CTGGGTGTCC
  301 ATGCGGGCCA GGTGAAAAGA CCTTGATCTT AACCTGGGTG ATGAGGTCTC GGTTAAAGGT
  361 GCCGTCTCGC GGCCATCCGA CGTTAAAGGT TGGCCATTCT GCAGAGCAGA AGGTAACCCA
  421 ACGTCTCTTC TTGACATCTA CCGACTGGTT GTGAGCGATC CGCTCGACAT CTTTCCAGTG
  481 ACCTAAGGTC AAACTTAAGG GAGTGGTAAC AGTCTGGCCC TAATTTTCAG ACAAATACAG
  541 AAACACAGTC AGACAGAGAC AACACAGAAC GATGCTGCAG CAGACAAGAC GCGCGGCGCG
  601 GCTTCGGTCC CAAACCGAAA GCAAAAATTC AGACGGAGGC GGGAACTGTT TTAGGTTCTC
  661 GTCTCCTACC AGAACCACAT ATCCCTCCTA TTAGGGGGGT GCACCAAAGA GTCCAAAACG
  721 ATCGGGATTT TTGGACTCAG GTCGGGCCAC AAAAACGGCC CCCGAAGTCC CTGGGACGTC
  781 TCCCAGGGTT GCGGCCGGGT GTTCCGAACT CGTCAGTTCC ACCACGGGTC CGCCAGATAC
  841 AGAGCTAGTT AGCTAACTAG TACAGACGCA GGCGCAAACA TTAGATGCCG GCACAAACAC
  901 GCACAAAGAC AGAGACAGAC ACGAAAACGA CCGCTGGCCA GCTTACCTCC CGGCGGGGGG
  961 GGTCGGTGGT CCCTGGGGCA GGGGTCTCCA AATCTCGGGT GGAACCTCCA AATGAAAAGA
 1021 ACCCCCGAAG GTGGGCAGTC AATCAAATCT GGAGAGAACC CTCCCAAGGA ATCAGCGAGT
 1081 CAACGAATCG GATGCAACCA GCCAAGAAAG CTTATGGAAT ACGGGTAACC GGCCGAACTC
1141 AAGTCAACTC CGAAGGAACC TTTG
//
Primer: EGFP-C RDB12177_A6Ajf.seq
>12177_12177_A6Aj_EGFP-C_G09_21.ab1
    1 CAGGCACTCT CGGGCATGGA CGAGCTGTAC AAGTAAAGCG GCCGGCCGCC AGCACAGTGG
   61 TCGACCGGGC CGCCAGCACA GTGGTCGATC GAGGGGGGGC CCGCGATTAG TCCAATTTGT
  121 TAAAGACAGG ATATCAGTGG TCCAGGCTCT AGTTTTGACT CAACAATATC ACCAGCTGAA
  181 GCCTATAGAG TACGAGCCAT AGATAGAATA AAAGATTTTA TTTAGTCTCC AGAAAAAGGG
  241 GGGAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT TAAGTAACGC CATTTTGCAA
  301 GGCATGGAAA ATACATAACT GAGAATAGAG AAGTTCAGAT CAAGGTTAGG AACAGAGAGA
  361 CAGCAGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCAGGGC
  421 CAAGAACAGT TGGAACAGCA GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT
  481 GCCCCGGCTC AGGGCCAAGA ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC
  541 TAGAGAACCA TCAGATGTTT CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT
  601 TTGAACTAAC CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC
  661 AATAAAAGAG CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC
  721 GGGTACCCGT GTATCCAAAT AAACCCTCTT GCAGTTGCAT CCGACTTGTG GTCTCGCTGT
  781 TCCTTGGGAG GGGTCTCCTC TGAGTGATTG ACTACCCGTC ACGGGGGTCT TACACATGCT
  841 GCAGTATCAA AATTAAATTG GGTTTTTCTT CTTAACTGTA AACATTACAT GAGCAAAATT
901 GGCATAC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

2019.04.09

GNP_filter3_RDBDEP_html_190327.pl