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pcDNA3.1-pA83-NDR1 (#RDB12199)

Expression clone of mouse Ndr1

Clone info. Expression clone of mouse Ndr1.
Comment The expression vector has a stretch of poly(A).
Vector backbone pcDNA3.1-pA83 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Mouse Ndr1|Stk38
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12199 pcDNA3.1-pA83-NDR1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-pA83-NDR1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12199).

Reference section:

Nishioka, N., Inoue, K., Adachi, K., Kiyonari, H., Ota, M., Ralston, A., Yabuta, N., Hirahara, S., Stephenson, R.O., Ogonuki, N., Makita, R., Kurihara, H., Morin-Kensicki, E.M., Nojima, H., Rossant, J., Nakao, K., Niwa, H., Sasaki, H., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12199_A6Ajp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB12199_A6Aja.seq check
>12199_12199_A6Aj_CMV-Forward_E08_14.ab1
    1 GCGTCCACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGTTAAGCTT ATGGCAATGA CAGGATCAAC
  181 ACCTTGCTCA TCCATGAGTA ATCATACGAA GGAGAGGGTG ACAATGACCA AAGTGACACT
  241 GGAGAATTTT TATAGCAACC TTATCGCTCA ACATGAAGAA CGAGAAATGA GACAAAAGAA
  301 GTTAGAAAAG GTGATGGAAG AAGAAGGCCT GAAAGACGAA GAGAAACGAC TCAGAAGATC
  361 CGCGCACGCT CGGAAGGAAA CAGAGTTTCT TCGATTAAAG AGAACAAGGC TTGGACTGGA
  421 AGATTTTGAG TCTTTAAAAG TCATAGGCCG AGGAGCATTT GGTGAGGTGC GGCTTGTTCA
  481 GAAGAAAGAC ACAGGGCATG TGTACGCAAT GAAAATCCTG CGCAAAGCAG ACATGCTCGA
  541 GAAGGAGCAG GTTGGCCACA TTCGTGCGGA GCGTGACATT CTAGTGGAGG CAGACAGTTT
  601 GTGGGTTGTG AAAATGTTCT ATAGTTTTCA GGATAAGCTA AACCTCTACC TAATCATGGA
  661 GTTCCTGCCT GGAGGGGACA TGATGACTTT ATTGATGAAA AAAGATACTC TGACAGAAGA
  721 GGAGACTCAG TTTTATATAG CAGAGACAGT ATTAGCCATA GACTCCATTC ACCAGCTGGG
  781 ATTCATCCAC AGAGATATCA AGCCAGACAA CCTTCTCTTG GACAGCAAGG GCCATGTGAA
  841 ACTTTCCGAC TTTGGCCTTT GCACAGGCCT GAAAAAAGCA CACAGGACAG AATTTTATAG
  901 GAACCTGAAC CACAGCCTGC CCAGTGATTT CACTTTTCAG AACATGAATT CCAAAGGAAA
  961 GCAGAGACCT GGAAAAGGAA CAGACGCCAG CTAGCCCTTC TCTACAGTGG GCACTCCTGA
 1021 CTACATTGCT CCCAGAGGTG TTCATGCAGA CGGTTTACAC CAAGCTCTGC GATTGGATGG
1081 TCGCTCGGGG TATTCATGTA TGAGATGCTC ATCGGCTTAC CCACCAATTC TGGCCTTCG
//
Primer: GNP_primer#10
Sequence file: RDB12199_A6Ajb.seq check
>12199_12199_A6Aj_GNPprimer#10_F08_17.ab1
    1 TTATTTGTGC CATTCATTTA AAAGATAACG TTTACAGGTT CTTTGGTGCC CATAGTCGAG
   61 CGGCCGCTAT TTTGCTGCTT TCATGTACGA AGGTATGGCC CCCCTGGCTG TCAGGCCCTC
  121 GAAGCGCTTG TATGTGTAAT TGATGAAGAC CCAGTCTTTG TTCTTGTAGT CCGTCTCAGG
  181 ATGACTACTT GTGGTCACTG TGGGCTTAAG AATATCAGAC TCTGGAAATT CATCGAAGTT
  241 TGAGGTATCA TCGATGCTCT TGATTTCAAT AGATATCGCA GCTGGTCTCT CCCTGATGTG
  301 TTCCCAGTCA ACACCTTCAA AAAAAAGATT ATTTTTTATT TCTTCAACTC CAGGGGCTCC
  361 GATTCTATGT TCCCATTCAC AGCAGAACCT CAGAATCAGG CCCTTGGCTT TCTCGGAGAC
  421 AGGAACTTCT GGAGGAAAGG TCAAAGTTTC CTTCCAGTTC ATCACTTTCT TGTATGTCTC
  481 TTGTGGGGTC TCGGAGCAGA ATGGTGGGTA GCCGATGAGC ATCTCATACA TGATTACCCC
  541 GAGCGACCAC CAATCGCAGA GCTTGTTGTA ACCCGTCTGC ATGAACACCT CTGGAGCAAT
  601 GTAGTCAGGA GTGCCCACTG TAGAGAAGGC TAGCTGGCGT CTGTTTCTTT TCCAGGTCTC
  661 TGCTTTCCTT TTGGAATTCA TGTTCTGAAA AGTGAAATCA CTGGGCAGGC TGTGGTTCAG
  721 GTTCCTATAA AATTCTGTCC TGTGTGCTTT TTTCAGGCCT GTGCAAAGGC CAAAGTCGGA
  781 AAGTTTCACA TGGCCCTTGC TGTCCAAGAG AAGGTTGTCT GGCTTGATAT CTCTGTGGAT
  841 GAATCCCAGC TGGTGAATGG AGTCTATGGC TAATACTGTC TCTGCTATAT AAAACTGAGT
  901 CTCCTCTTCT GTCAGAGTAT CTTTTTTTCA TCAATAAAGT CATCATGTCC CCTCCAGGCA
  961 GGAACTCCAT GATTAGGTAG AGGTTTAGCT TTATCCCTGA AAACTATAGA ACATTTTCAC
 1021 ACCCCACAAA CTGTCTGCCT CCACTAGATG TCACGCTCCG CACGAATGTC TGACTTGCCT
 1081 TGATCTCGAG CCATGTCTGC GTTGCGCAGG AATTTTCAAT TGCGTTAACA CAATGTCCCA
1141 TGGGTGATCC ATAT
//
Primer: BGH_rev2
Sequence file: RDB12199_A6Ajc.seq check
>12199_12199_A6Aj_BGH_rev2_G08_20.ab1
    1 CATCATGGCT AGGAGGCCGG GGGAGGGGCA ACAACAGATG GCTGGCAACT AGAAGGCACA
   61 GTCGAGGCTG ATCAGCGGGT TTAAACTCAA TGGTGATGGT GATGATGACC GGTATGCATA
  121 TTCAGATCCT CTTCTGAGAT GAGTTTTTGT TCGAAGGGCC CTCTAGACTC GAGGTACGCC
  181 ACAGCGTATG ATGCGTATTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
  241 TTTTTTTTTT TTTTTTTTTT TTTTTTTTCC TTTCCCCACC TTTTTTTTGG CCCCCTTCAT
  301 TTTAAAAAAA AAACTTTTAC GGGTTTTTTG GGGCCCAAAA CCAAGGGGCC CCTTTTTTTC
  361 TTTTTTTTTG TAAAAAGGGG GGCCCCCCCG GGGTGTCGGG CCCCCAAACC CCTTTTTTGG
  421 TTAATTGAAA AAAACCCCCT TTTTTTTTTT TTATCCCCCC CCAGGGAAAA CCTTTGGGGC
  481 CCCGGGGGGC TTAAAAAAAC CAAACCCGGG AAATTCCCCA AATTTTGGGG GACCCCCCGG
  541 CCCCTTGTTT TCAAAAAAAA CCCCCCCGGG CCCCCCCCGG GGGGTTCCCC CCCCACCCCC
  601 TTCAAAAAAA AAATTTTTTT TTTTTTTTTC ACCCCCGGGG GCTCCAATTC TTTTTTCCCT
  661 TTCCAAAAAA AACCCCAAAA TCGGGCCCTT GGTTTTTTCG AAAAAGGGAA TTTTGGGAGG
  721 AAAGGGCAAA TTTCCCTCCC AGTCCCCATT TTTTGGATGT CCCTTGGGGG GCCCCGAAAC
  781 AAAAGGGGGG GGACCCAAAA ACCCCCCATA CATGATTCCC CCGAGCCCCC CCCATTCCAA
  841 AGTTTTTTGT ACCCCCTGGG GTAAACCCCC GGAACATTGT ATCCCGGGAG GGCCCCCGTA
  901 AAAAAAGCCA ACTGGGCGCT GTTTTTCTTT TTCAGGGCCC TCCGGCTTTC TCTTTTGAGA
  961 AATTCTGGTC TCAGAAAAGT GTAATCCCCT GGGCAGGCTG TGGGTTCAGG TCTCATTAAA
 1021 CTCGCGCGCG GGGGGGCTTT TTACAGCGCG TGGTGCAGAG CCAAAGTCTC GAAAAGTTTT
 1081 CCCATTGCTT GCTCGTACAA AAAGAATGTG TCTGGCTGAT ATCCTCGTGA GAATCAGCCT
1141 TGGTATGATC GTAT
//
Primer: SV40pro_F
Sequence file: RDB12199_A6Ajd.seq check
>12199_12199_A6Aj_SV40pro_F_C04_07.ab1
    1 CCTGGTCTGG ACATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
  121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
  661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
  841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  901 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGCG AAATGACCCG ACCAAGCGAC
  961 GCCCAACCTG CATCACGAGA TTTCGATTCA CGCGCGTCCT ATGAAAGTTG GCCTTCGAAT
 1021 CGTTTCAGGA CGCGGCTTGC ATGATCTCAG CGCGGGGATC TCATGCTGAG TCTCGCCACC
1081 GAACCTGGCT TTATTTGCAA GCT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. [link to RRC of NBRP]