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pcDNA3.1-pA83-NDR1

Expression clone of mouse Ndr1

Catalog number RDB12199
Resource name pcDNA3.1-pA83-NDR1
Clone info. Expression clone of mouse Ndr1.
The expression vector has a stretch of poly(A).
Vector backbone pcDNA3.1-pA83 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Mouse Ndr1|Stk38
Depositor Sasaki, Hiroshi |
 
Other clones in our bank mouse Stk38 (NCBI Gene 106504) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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RDB12199 pcDNA3.1-pA83-NDR1 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12199_A6Ajp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB12199_A6Aja.seq
>12199_12199_A6Aj_CMV-Forward_E08_14.ab1
    1 GCGTCCACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGTTAAGCTT ATGGCAATGA CAGGATCAAC
  181 ACCTTGCTCA TCCATGAGTA ATCATACGAA GGAGAGGGTG ACAATGACCA AAGTGACACT
  241 GGAGAATTTT TATAGCAACC TTATCGCTCA ACATGAAGAA CGAGAAATGA GACAAAAGAA
  301 GTTAGAAAAG GTGATGGAAG AAGAAGGCCT GAAAGACGAA GAGAAACGAC TCAGAAGATC
  361 CGCGCACGCT CGGAAGGAAA CAGAGTTTCT TCGATTAAAG AGAACAAGGC TTGGACTGGA
  421 AGATTTTGAG TCTTTAAAAG TCATAGGCCG AGGAGCATTT GGTGAGGTGC GGCTTGTTCA
  481 GAAGAAAGAC ACAGGGCATG TGTACGCAAT GAAAATCCTG CGCAAAGCAG ACATGCTCGA
  541 GAAGGAGCAG GTTGGCCACA TTCGTGCGGA GCGTGACATT CTAGTGGAGG CAGACAGTTT
  601 GTGGGTTGTG AAAATGTTCT ATAGTTTTCA GGATAAGCTA AACCTCTACC TAATCATGGA
  661 GTTCCTGCCT GGAGGGGACA TGATGACTTT ATTGATGAAA AAAGATACTC TGACAGAAGA
  721 GGAGACTCAG TTTTATATAG CAGAGACAGT ATTAGCCATA GACTCCATTC ACCAGCTGGG
  781 ATTCATCCAC AGAGATATCA AGCCAGACAA CCTTCTCTTG GACAGCAAGG GCCATGTGAA
  841 ACTTTCCGAC TTTGGCCTTT GCACAGGCCT GAAAAAAGCA CACAGGACAG AATTTTATAG
  901 GAACCTGAAC CACAGCCTGC CCAGTGATTT CACTTTTCAG AACATGAATT CCAAAGGAAA
  961 GCAGAGACCT GGAAAAGGAA CAGACGCCAG CTAGCCCTTC TCTACAGTGG GCACTCCTGA
 1021 CTACATTGCT CCCAGAGGTG TTCATGCAGA CGGTTTACAC CAAGCTCTGC GATTGGATGG
1081 TCGCTCGGGG TATTCATGTA TGAGATGCTC ATCGGCTTAC CCACCAATTC TGGCCTTCG
//
Primer: GNP_primer#10 RDB12199_A6Ajb.seq
>12199_12199_A6Aj_GNPprimer#10_F08_17.ab1
    1 TTATTTGTGC CATTCATTTA AAAGATAACG TTTACAGGTT CTTTGGTGCC CATAGTCGAG
   61 CGGCCGCTAT TTTGCTGCTT TCATGTACGA AGGTATGGCC CCCCTGGCTG TCAGGCCCTC
  121 GAAGCGCTTG TATGTGTAAT TGATGAAGAC CCAGTCTTTG TTCTTGTAGT CCGTCTCAGG
  181 ATGACTACTT GTGGTCACTG TGGGCTTAAG AATATCAGAC TCTGGAAATT CATCGAAGTT
  241 TGAGGTATCA TCGATGCTCT TGATTTCAAT AGATATCGCA GCTGGTCTCT CCCTGATGTG
  301 TTCCCAGTCA ACACCTTCAA AAAAAAGATT ATTTTTTATT TCTTCAACTC CAGGGGCTCC
  361 GATTCTATGT TCCCATTCAC AGCAGAACCT CAGAATCAGG CCCTTGGCTT TCTCGGAGAC
  421 AGGAACTTCT GGAGGAAAGG TCAAAGTTTC CTTCCAGTTC ATCACTTTCT TGTATGTCTC
  481 TTGTGGGGTC TCGGAGCAGA ATGGTGGGTA GCCGATGAGC ATCTCATACA TGATTACCCC
  541 GAGCGACCAC CAATCGCAGA GCTTGTTGTA ACCCGTCTGC ATGAACACCT CTGGAGCAAT
  601 GTAGTCAGGA GTGCCCACTG TAGAGAAGGC TAGCTGGCGT CTGTTTCTTT TCCAGGTCTC
  661 TGCTTTCCTT TTGGAATTCA TGTTCTGAAA AGTGAAATCA CTGGGCAGGC TGTGGTTCAG
  721 GTTCCTATAA AATTCTGTCC TGTGTGCTTT TTTCAGGCCT GTGCAAAGGC CAAAGTCGGA
  781 AAGTTTCACA TGGCCCTTGC TGTCCAAGAG AAGGTTGTCT GGCTTGATAT CTCTGTGGAT
  841 GAATCCCAGC TGGTGAATGG AGTCTATGGC TAATACTGTC TCTGCTATAT AAAACTGAGT
  901 CTCCTCTTCT GTCAGAGTAT CTTTTTTTCA TCAATAAAGT CATCATGTCC CCTCCAGGCA
  961 GGAACTCCAT GATTAGGTAG AGGTTTAGCT TTATCCCTGA AAACTATAGA ACATTTTCAC
 1021 ACCCCACAAA CTGTCTGCCT CCACTAGATG TCACGCTCCG CACGAATGTC TGACTTGCCT
 1081 TGATCTCGAG CCATGTCTGC GTTGCGCAGG AATTTTCAAT TGCGTTAACA CAATGTCCCA
1141 TGGGTGATCC ATAT
//
Primer: BGH_rev2 RDB12199_A6Ajc.seq
>12199_12199_A6Aj_BGH_rev2_G08_20.ab1
    1 CATCATGGCT AGGAGGCCGG GGGAGGGGCA ACAACAGATG GCTGGCAACT AGAAGGCACA
   61 GTCGAGGCTG ATCAGCGGGT TTAAACTCAA TGGTGATGGT GATGATGACC GGTATGCATA
  121 TTCAGATCCT CTTCTGAGAT GAGTTTTTGT TCGAAGGGCC CTCTAGACTC GAGGTACGCC
  181 ACAGCGTATG ATGCGTATTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
  241 TTTTTTTTTT TTTTTTTTTT TTTTTTTTCC TTTCCCCACC TTTTTTTTGG CCCCCTTCAT
  301 TTTAAAAAAA AAACTTTTAC GGGTTTTTTG GGGCCCAAAA CCAAGGGGCC CCTTTTTTTC
  361 TTTTTTTTTG TAAAAAGGGG GGCCCCCCCG GGGTGTCGGG CCCCCAAACC CCTTTTTTGG
  421 TTAATTGAAA AAAACCCCCT TTTTTTTTTT TTATCCCCCC CCAGGGAAAA CCTTTGGGGC
  481 CCCGGGGGGC TTAAAAAAAC CAAACCCGGG AAATTCCCCA AATTTTGGGG GACCCCCCGG
  541 CCCCTTGTTT TCAAAAAAAA CCCCCCCGGG CCCCCCCCGG GGGGTTCCCC CCCCACCCCC
  601 TTCAAAAAAA AAATTTTTTT TTTTTTTTTC ACCCCCGGGG GCTCCAATTC TTTTTTCCCT
  661 TTCCAAAAAA AACCCCAAAA TCGGGCCCTT GGTTTTTTCG AAAAAGGGAA TTTTGGGAGG
  721 AAAGGGCAAA TTTCCCTCCC AGTCCCCATT TTTTGGATGT CCCTTGGGGG GCCCCGAAAC
  781 AAAAGGGGGG GGACCCAAAA ACCCCCCATA CATGATTCCC CCGAGCCCCC CCCATTCCAA
  841 AGTTTTTTGT ACCCCCTGGG GTAAACCCCC GGAACATTGT ATCCCGGGAG GGCCCCCGTA
  901 AAAAAAGCCA ACTGGGCGCT GTTTTTCTTT TTCAGGGCCC TCCGGCTTTC TCTTTTGAGA
  961 AATTCTGGTC TCAGAAAAGT GTAATCCCCT GGGCAGGCTG TGGGTTCAGG TCTCATTAAA
 1021 CTCGCGCGCG GGGGGGCTTT TTACAGCGCG TGGTGCAGAG CCAAAGTCTC GAAAAGTTTT
 1081 CCCATTGCTT GCTCGTACAA AAAGAATGTG TCTGGCTGAT ATCCTCGTGA GAATCAGCCT
1141 TGGTATGATC GTAT
//
Primer: SV40pro_F RDB12199_A6Ajd.seq
>12199_12199_A6Aj_SV40pro_F_C04_07.ab1
    1 CCTGGTCTGG ACATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
  121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
  661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
  841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  901 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGCG AAATGACCCG ACCAAGCGAC
  961 GCCCAACCTG CATCACGAGA TTTCGATTCA CGCGCGTCCT ATGAAAGTTG GCCTTCGAAT
 1021 CGTTTCAGGA CGCGGCTTGC ATGATCTCAG CGCGGGGATC TCATGCTGAG TCTCGCCACC
1081 GAACCTGGCT TTATTTGCAA GCT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085.

2019.04.09

GNP_filter3_RDBDEP_html_190327.pl