Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3.1-pA83-Lats2 (#RDB12200)

Expression clone of mouse Lats2 cDNA

Clone info. Expression clone of mouse Lats2 cDNA.C
Comment The expression vector has a stretch of poly(A).
Vector backbone pcDNA3.1-pA83 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Mouse Lats2 cDNA
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12200 pcDNA3.1-pA83-Lats2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-pA83-Lats2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12200).

Reference section:

Nishioka, N., Inoue, K., Adachi, K., Kiyonari, H., Ota, M., Ralston, A., Yabuta, N., Hirahara, S., Stephenson, R.O., Ogonuki, N., Makita, R., Kurihara, H., Morin-Kensicki, E.M., Nojima, H., Rossant, J., Nakao, K., Niwa, H., Sasaki, H., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12200_A4Fup1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,insert5'
Sequence file: RDB12200_A4Fua.seq check
>12200_12200_A4Fu_CMV-Foward_D08_11.ab1
    1 ACGGCCACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGTTAAGCTT GCCGCCACCA TGTACCCATA
  181 CGACGTTCCA GATTACGCTG GTACCATGAG GCCAAAGACT TTTCCTGCCA CAACTTACTC
  241 TGGAAATAGC CGGCAGCGAT TGCAAGAGAT TCGAGAGGGG CTGAAGCAGC CATCCAAGGC
  301 TTCCACCCAG GGGCTGCTGG TGGGACCAAA CAGTGACACT TCCCTGGATG CCAAAGTCCT
  361 GGGGAGCAAA GATGCCTCCA GGCAGCAGCA AATGAGAGCC ACCCCGAAGT TTGGACCTTA
  421 TCAAAAAGCT CTCAGGGAAA TCCGATATTC CCTCCTGCCT TTTGCCAACG AGTCAGGCAC
  481 TTCGGCAGCT GCAGAGGTGA ACCGGCAGAT GCTTCAGGAG TTGGTGAATG CGGGATGTGA
  541 CCAGGAGATG GCTGGCAGAG CGCTCAAGCA GACGGGCAGT AGGAGTATCG AAGCTGCCTT
  601 GGAGTACATC AGTAAGATGG GCTACCTGGA CCCCAGGAAT GAGCAGATTG TGCGAGTCAT
  661 CAAGCAGACC TCCCCAGGAA AGGGCCTGGC GCCCACCCCG GTGACTCGGC GGCCCAGTTT
  721 CGAGGGCACA GGGGAAGCAC TCCCATCCTA CCACCAGCTG GGTGGTGCAA ACTACGAGGG
  781 CCCCGCCGCA CTGGAGGAGA TGCCGCGGCA ATATTTAGAC TTTCTCTTCC CTGGAGCCGG
  841 AGCCGGCACC CACGGTGCCC AGGCTCACCA GCATCCTCCC AAAGGGTACA GCACAGCAGT
  901 AGAGCCAAGT GCGCACTTTC CGGGCACACA CTATGGTCGT GGTCATCTAC TATCGGAGCA
  961 GCCTGGGTAT GGGGGTGCAG CGCAGTTTCC TCCCTTCCAG AACAAGACGC CACCAGATGC
 1021 CCTATTCCCA GCATGGCCAA GACCAGGTGG CCTCCCGCCA GCTCACTTTC TGCCCATGCT
1081 GGGCTGTACA CTGGCTCGCA CACAGGCCGG CGGCTACCCC CCACCCTTGA AG
//
Primer: GNP_primer#10 (Pr0763)
Region: insert3'
Sequence file: RDB12200_A4Fub.seq check
>12200_12200_A4Fu_GNP_primer#10_E08_14.ab1
    1 TTATTGTGCC ATTCATTTAA AAGATAACGT TTACAGGTTC TTTGGTGCCC ATAGTCGAGC
   61 GGCCGCCACT GTGCTGGATA TCTGCAGAAT TCTTACACGT ACACCGGCTG GCAGCCCTCG
  121 GCCGCACCTT CCAAGTCCGC ATCCCCTGGG TCTGCACTCT CTGCGGGCTC TGAGGGCTTC
  181 GGGCACCGGA AGGGATAGCC GTTGTCATCG AAGAACCTGC GGAAGGTGAA CTCATAGAAG
  241 GCGTGCTCTG GATGCTTGCT GCTGGGGGAG GCCAGCGTGT CCCAGGCCTT GGCGCTCTCT
  301 CCGCTGGCCT CGTGCCAGGG GCTTTCTTCA TCCACCGGGT CAAAATTGGA GGTGTCCATG
  361 GGGTGGCTGA TGGTGGGGAC GTAGGGTGCA GGCTGCTTTC GGATGTCACG GGAAAAGTCG
  421 ATGGTGTTGA AGAACGGGTG TGCCTTGAGG TCATCTGCCC CATCCCTGCC CAGGCGGCAG
  481 TCAGCCGCGC AGCACAGCTT CGTGATGAGG TCTCGGGCCT CAGCGCTGAG CCTCACCTGC
  541 GTAGGGATAT GCAGCGTGCT CTCCCAGTTG ATCACCTTCA GCTGCGTCTC TGTGGGGGTG
  601 GGGGCCAAGA AAGGCGGCTG CCCAACCAGC ATCTCAAAGA GAATCACACC GACGCTCCAC
  661 CAGTCACAGA GCTGCGTGTA CCCTTTGCGG AGAAGCACCT CCGGAGCGAT GTAATTTGGT
  721 GTCCCGACAA GAGAATGTGC CAGGCACCTC TGGTGCTGCT TCTGCGCCCT CTGCTCCAGG
  781 GTCTTTAACC TGTCTCCACA GCGACAGTTG GAAACATCGT CCCAGAGGTC ACCGGGCTCC
  841 ATGCTGTCCT GTCTCATGTG GTTCCCTTTC TGGTAGTACT TGGAATTGTG AGTCCACCTG
  901 AATCCAGTGC AGAGGCCAAA ATCTGTCAGC TTAATATGAC CATCCAGGTC GATGAGTATG
  961 TTGTCAGGCT TGATGTCCCG GGTGGGATAA AGCCCATCTT TGTGGACACT TTCATGACCA
 1021 GGGTCAACTC TGCATGTAGA GGCGGGCCAG TTGCTCAAGG AGGACTCATC CCTGAATCAG
1081 CAGGCTTCAT TCATATTCCT CGGCCTGGTA TGTTAGGTTC CAATTCCA
//
Primer: Lats2#11 (Pr0039)
Region: insert mid
Sequence file: RDB12200_A4Fud.seq check
>12200_12200_A4Fu_Lats2#11_B02_05.ab1
    1 CGAAGGGTTC AGCCGGAAGC TACAGCTGGA GCAGGAAATG GCCAAAGCTG GGCTCTGTGA
   61 GGCCGAGCAG GAGCAGATGA GGAAGATCCT CTACCAGAAG GAGTCTAACT ACAACCGGCT
  121 GAAGAGGGCC AAGATGGACA AGTCCATGTT TGTGAAAATC AAGACTCTAG GCATCGGTGC
  181 CTTTGGGGAA GTGTGCCTCG CTTGTAAGCT GGACACTCAC GCTCTGTACG CCATGAAGAC
  241 TCTCAGGAAG AAGGATGTCC TGAACCGGAA TCAAGTGGCC CATGTCAAGG CTGAGAGGGA
  301 CATCCTGGCT GAAGCAGACA ATGAGTGGGT GGTCAAACTC TACTACTCCT TCCAGGACAA
  361 GGACAGCCTG TACTTTGTGA TGGACTACAT ACCAGGCGGG GATATGATGA GCCTGCTGAT
  421 CAGGATGGAG GTCTTCCCTG AGCACCTGGC CCGCTTCTAC ATTGCAGAGT TGACCCTGGC
  481 CATTGAAAGT GTCCACAAGA TGGGCTTTAT CCACCGGGAC ATCAAGCCTG ACAACATACT
  541 CATCGACCTG GATGGTCATA TTAAGCTGAC AGATTTTGGC CTCTGCACTG GATTCAGGTG
  601 GACTCACAAT TCCAAGTACT ACCAGAAAGG GAACCACATG AGACAGGACA GCATGGAGCC
  661 CGGTGACCTC TGGGACGATG TTTCCAACTG TCGCTGTGGA GACAGGTTAA AGACCCTGGA
  721 GCAGAGGGCG CAGAAGCAGC ACCAGAGGTG CCTGGCACAT TCTCTTGTCG GGACACCAAA
  781 TTACATCGCT CCGGAGGTGC TTCTCCGCAA AGGGTACACG CAGCTCTGTG ACTGGTGGAG
  841 CGTCGGTGTG ATTCTCTTTG AGATGCTGGT TGGGCAGCCG CCTTTCTTGG CCCCCACCCC
  901 CCACAGAGAC GCAGCTGAAG GTGATCAACT GGGAGAGCAC GCTGCATATC CCTACGCAGG
  961 TGAGGCTCAG CGCTGAGGCC CGAGACCTCA TCACGAAGCT GTGCTGCGCG GCTGACTGCC
 1021 CGCCTTGAGC AGGATGGGGG CAGATGACCC TCAAGCACAC CCGTTCTTTC AACACCATCG
1081 ACTTTTCCGT TGACATCCTA AGGCAGCCTG GCCACCCCCT TTACGG
//
Primer: BGH_rev (Pr0009)
Region: TRF2-3'UTR+polyA83
Sequence file: RDB12200_A4Fuc.seq check
>12200_12200_A4Fu_BGHrev_F08_17.ab1
    1 GCAAGGAGTC ATGGTGATGG TGATGATGAC CGGTATGCAT ATTCAGATCC TCTTCTGAGA
   61 TGAGTTTTTG TTCGAAGGGC CCTCTAGACT CGAGGTACGC CACAGCGTAT GATGCGTATT
  121 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
  181 TTTTTTTCCT TTCCCAAATT TTTTTTTGGG CCCTTTCATT TAAAAAAAAA AAGTTTTACG
  241 GGTTTTTGGG GCCCCAAAGC CAAGGGGCCC CCCTGGGGGG GAATTTTGGA AAAATTTTTA
  301 CCCAAACCCG GGGGGGGGCC CCTGGGCCCC CCCTTCAAAG CCCCCCCCCC GGGGGTCGGC
  361 CCCCTCGGGG GGTTTGGGGG CTTCCGGCCC CCAAAAGGAA AACCCTTTTC CCCCAAAAAC
  421 CCGGGGAAGG GGAAACCCTA AAAAGGGGTC CTTTGATTTT TTTTTGTGGG GGGAGGCCAG
  481 GGTTCCCCAG GCTTGGGCCT TTTCCCCGTG GGCCCCGGGC CGGGGGTTTT TTTCCCCCCG
  541 GGGAAAAAAT TGGGGGGGCC CTGGGGGGGG TTAAGGGGGG GAACTAAGGG GGCAGGTTTT
  601 TTTCGAATTT ACGGGAAAAT TCAAGGTTTT TAAAAAGGTT TGCCTTTGGG GCCTTTTCCC
  661 TCTCTGCCCC GGCGGAATCC CCGCACAACA CCTCCATGAG AGTCTGGGCT CAAGCTAACC
  721 TCCTCGTAGG ATATGCGGGC TCTCCAGTGA CCCCCTCCAC TGCCTCTCTG TGGGTGGGGC
  781 AAGAAGCGTT CTACCGATTC AAGAGATTCA CGACTCTCCA TATCGAGGTG TGTACCTTGC
  841 GAGAAGCCTC CGACCAGTAC TCGGTCGCAG AGTGTCAGAC TTGTTGTTCG CCTGGCCCAA
901 GACTACGTTC CAAAGAAGGT GACCTGTCAG GTACCGGCAT CAG
//
Primer: SV40pro_F (Pr0110)
Region: SV40pro_ori,NeoR
Sequence file: RDB12200_A4Fue.seq check
>12200_12200_A4Fu_SV40pro_F_F10_16.ab1
    1 CCTGGTCCTT TGACATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG CGAAATGACC GACCAAGCGA
  961 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCCG CCTTCTATGA AAGGTTGGGC
 1021 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG GATGATCCTC AGCGCGGGGA TCTCATGCTG
 1081 GAGTTCTTCG CCCCACCCCC ACTGCTTATT GCAGCTTATA TGTACAATTA AGGCATTAGC
1141 ATCACTAATT CACAATTAGG CCATTTTTTT CCACTGCATC CTG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. [link to RRC of NBRP]
reference Tsutsumi, R., YAP and TAZ, Hippo signaling targets, act as a rheostat for nuclear SHP2 function. Dev. Cell, 26 (6): 658-665 (2013). PMID 24035415. [link to RRC of NBRP]