Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCMV/SV-Flag-Lats2-KD

Expression clone of mouse Lats2, kinase dead variant

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12214_A6Ajp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB12214_A6Aja.seq
>12214_12214_A6Aj_CMV-Forward_A02_02.ab1     1 AGTGGATTAA CCTCCCGGGC CCCATTTGAA CGCAATGGGC GGTAGGCGTG TACGGTGGGA    61 GGTCTATATA AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA   121 AATTAATACG ACTCACTATA GGGAGACCCA AGCTTCCACC ATGGACTACA AGGACGACGA   181 TGACAAGGGA TCCATGAGGC CAAAGACTTT TCCTGCCACA ACTTACTCTG GAAATAGCCG   241 GCAGCGATTG CAAGAGATTC GAGAGGGGCT GAAGCAGCCA TCCAAGGCTT CCACCCAGGG   301 GCTGCTGGTG GGACCAAACA GTGACACTTC CCTGGATGCC AAAGTCCTGG GGAGCAAAGA   361 TGCCTCCAGG CAGCAGCAAA TGAGAGCCAC CCCGAAGTTT GGACCTTATC AAAAAGCTCT   421 CAGGGAAATC CGATATTCCC TCCTGCCTTT TGCCAACGAG TCAGGCACTT CGGCAGCTGC   481 AGAGGTGAAC CGGCAGATGC TTCAGGAGTT GGTGAATGCG GGATGTGACC AGGAGATGGC   541 TGGCAGAGCG CTCAAGCAGA CGGGCAGTAG GAGTATCGAA GCTGCCTTGG AGTACATCAG   601 TAAGATGGGC TACCTGGACC CCAGGAATGA GCAGATTGTG CGAGTCATCA AGCAGACCTC   661 CCCAGGAAAG GGCCTGGCGC CCACCCCGGT GACTCGGCGG CCCAGTTTCG AGGGCACAGG   721 GGAAGCACTC CCATCCTACC ACCAGCTGGG TGGTGCAAAC TACGAGGGCC CCGCCGCACT   781 GGAGGAGATG CCGCGGCAAT ATTTAGACTT TCTCTTCCCT GGAGCCGGAG CCGGCACCCA   841 CGGTGCCCAG GCTCACCAGC ATCCTCCCAA AGGGTACAGC ACAGCAGTAG AGCCAAGTGC   901 GCACTTTCCG GGCACACACT ATGGGTCGTG GTCATCTACT ATCGGAGCAG CCTGGGTATG   961 GGGTGCAGCC GCAGTTCCTC CCTTCCAGAA CAGGACGCCA CCAGATGCCT TATTTCCAGC  1021 ATGGGCCAAG CCCCAAGGTG GGTCCCTCCC GCCAGCTCAC TTTTCCTGCC ATGCCTGGGC  1081 TGTACACTGC CTCGCACCCC ACAAGCTGGC GGCTAACCCC ACCTTGGGGC CCACCCAATT 1141 AACATGTATG TGTTTGGGGC AT //
Primer: GNP_primer#10 Sequence file: RDB12214_A6Ajb.seq
>12214_12214_A6Aj_pMAMneo_Reverse_E04_13.ab1     1 TGTCAGTATG TCACACCACA GAAGTAAGGT TCCTTCACAA AGATCCTCTA GCATTTAGGT    61 GACACTATAG AATAGGGCCC TCTAGATGCA TGCTCGAGTG CGGCCGCAAG CTTGTCGACG   121 GAGCTCGAAT TCGGATCATC TGCAGAATTT CGAGGTTACA CGTACACCGG CTGGCAGCCC   181 TCGGCCGCAC CTTCCAAGTC CGCATCCCCT GGGTCTGCAC TCTCTGCGGG CTCTGAGGGC   241 TTCGGGCACC GGAAGGGATA GCCATTGTCA TCGAAGAACC TGCGGAAGGT GAACTCATAG   301 AAGGCGTGCT CTGGATGCTT GCTGCTGGGG GAGGCCAGCG TGTCCCAGGC CTTGGCGCTC   361 TCTCCGCTGG CCTCGTGCCA GGGGCTTTCT TCATCCACCG GGTCAAAATT GGAGGTGTCC   421 ATGGGGTGGC TGATGGTGGG GACGTAGGGT GCAGGCTGCT TTCGGATGTC ACGGGAAAAG   481 TCGATGGTGT TGAAGAACGG GTGTGCCTTG AGGTCATCTG CCCCATCCCT GCCCAGGCGG   541 CAGTCAGCCG CGCAGCACAG CTTCGTGATG AGGTCTCGGG CCTCAGCGCT GAGCCTCACC   601 TGCGTAGGGA TATGCAGCGT GCTCTCCCAG TTGATCACCT TCAGCTGCGT CTCTGTGGGG   661 GTGGGGGCCA AGAAAGGCGG CTGCCCAACC AGCATCTCAA AGAGAATCAC ACCGACGCTC   721 CACCAGTCAC AGAGCTGCGT GTACCCTTTG CGGAGAAGCA CCTCCGGAGC GATGTAATTT   781 GGTGTCCCGA CAAGAGAATG TGCCAGGCAC CTCTGGTGCT GCTTCTGCGC CCTCTGCTCC   841 AGGGTCTTTA ACCTGTCTCC ACAGCGACAG TTGGAAACAT CGTCCCAGAG GTCACCGGGC   901 TCCATGCTGT CCTGTCTCAT GTGGTTCCCT TTCTGGTAGT ACTTGGAATT TGTGAGTCCA   961 CCCTGAATCC AGTGCAGAGG CCAAAAGCTG TCAGCTAATA TGACCATCCA GGGTCGATGA  1021 GTATGGTTGT CAGCTGAATG TCCCGATGAA TAAGGCCATC TGTGAACAAC TTTCATGGCC 1081 AGGATCACTC TGCCAATGGT AAAAAAGCGG GACCAGAGAT //
Primer: Lats2#11 Sequence file: RDB12214_A6Ajc.seq
>12214_12214_A6Aj_Lats2#11_H07_22.ab1     1 CAGAGGGCCA GCCGGGAGCT ACAGCTGGAG CAGGAAATGG CCAAAGCTGG GCTCTGTGAG    61 GCCGAGCAGG AGCAGATGAG GAAGATCCTC TACCAGAAGG AGTCTAACTA CAACCGGCTG   121 AAGAGGGCCA AGATGGACAA GTCCATGTTT GTGAAAATCA AGACTCTAGG CATCGGTGCC   181 TTTGGGGAAG TGTGCCTCGC TTGTAAGCTG GACACTCACG CTCTGTACGC CATGAAGACT   241 CTCAGGAAGA AGGATGTCCT GAACCGGAAT CAAGTGGCCC ATGTCAAGGC TGAGAGGGAC   301 ATCCTGGCTG AAGCAGACAA TGAGTGGGTG GTCAAACTCT ACTACTCCTT CCAGGACAAG   361 GACAGCCTGT ACTTTGTGAT GGACTACATA CCAGGCGGGG ATATGATGAG CCTGCTGATC   421 AGGATGGAGG TCTTCCCTGA GCACCTGGCC CGCTTCTACA TTGCAGAGTT GACCCTGGCC   481 ATTGAAAGTG TCCACAAGAT GGGCTTTATC CACCGGGACA TCAAGCCTGA CAACATACTC   541 ATCGACCTGG ATGGTCATAT TAAGCTGACA GCTTTTGGCC TCTGCACTGG ATTCAGGTGG   601 ACTCACAATT CCAAGTACTA CCAGAAAGGG AACCACATGA GACAGGACAG CATGGAGCCC   661 GGTGACCTCT GGGACGATGT TTCCAACTGT CGCTGTGGAG ACAGGTTAAA GACCCTGGAG   721 CAGAGGGCGC AGAAGCAGCA CCAGAGGTGC CTGGCACATT CTCTTGTCGG GACACCAAAT   781 TACATCGCTC CGGAGGTGCT TCTCCGCAAA GGGGTACACG CAGCTCTGTG ACTGGTGGAG   841 CGTCGGTGTG ATTCTCTTTG AGATGCTGGG TTGGGCAGCC GCCTTTCTTG GCCCCCACCC   901 CCACAGAGAC GCAGCTGAAA GGTGATCAAC TGGGAGAGCA CGCTGCATAT CCCTACGCAG   961 GTGAGGCTCA GCGCTGAGGC CCGAGACTCA TCACGAAGCT GTGCTGCGCG CTGACTGCCG  1021 CCTGGGCAGG AATGGGGGCA GATGACCTCA AGGCACACCC GGTTCTCAAC ACATCGAACC  1081 TATACCGTGA CATTCCGGAA GGCCAGGCCT GCACCCTTAC CGTTCGCAAC CCAATTTCAG 1141 ATCGCA //
Primer: Sequence file: RDB12214_A6Ajd.seq
1

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl