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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMV/SV-Flag-Lats2-KD

Expression clone of mouse Lats2, kinase dead variant

Catalog number RDB12214
Resource name pCMV/SV-Flag-Lats2-KD
Clone info. Expression clone of mouse Lats2 cDNA. Catalytically inactive (kinase dead) variant. cDNA fragment 437 to 3,565 nt of NM_015771.2 (CDS, 437..3565) is cloned. A to C substitution at 2,736 nt (Asp to Ala at 767th aa, introduced mutation).
Comment Known differences: C to T substitution at 3457 nt (Asn at 1007th aa, synonymous).
Vector backbone pCMV/SV-Flag1 (Plasmid)
Size of vector backbone 4.6 kb
Gene/insert name Mouse Lats2 cDNA
Depositor|Developer Sasaki, Hiroshi |
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External Database
Mouse Lats2

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
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          RDB12214 pCMV/SV-Flag-Lats2-KD DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12214_A6Ajp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB12214_A6Aja.seq check
          >12214_12214_A6Aj_CMV-Forward_A02_02.ab1
              1 AGTGGATTAA CCTCCCGGGC CCCATTTGAA CGCAATGGGC GGTAGGCGTG TACGGTGGGA
             61 GGTCTATATA AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA
            121 AATTAATACG ACTCACTATA GGGAGACCCA AGCTTCCACC ATGGACTACA AGGACGACGA
            181 TGACAAGGGA TCCATGAGGC CAAAGACTTT TCCTGCCACA ACTTACTCTG GAAATAGCCG
            241 GCAGCGATTG CAAGAGATTC GAGAGGGGCT GAAGCAGCCA TCCAAGGCTT CCACCCAGGG
            301 GCTGCTGGTG GGACCAAACA GTGACACTTC CCTGGATGCC AAAGTCCTGG GGAGCAAAGA
            361 TGCCTCCAGG CAGCAGCAAA TGAGAGCCAC CCCGAAGTTT GGACCTTATC AAAAAGCTCT
            421 CAGGGAAATC CGATATTCCC TCCTGCCTTT TGCCAACGAG TCAGGCACTT CGGCAGCTGC
            481 AGAGGTGAAC CGGCAGATGC TTCAGGAGTT GGTGAATGCG GGATGTGACC AGGAGATGGC
            541 TGGCAGAGCG CTCAAGCAGA CGGGCAGTAG GAGTATCGAA GCTGCCTTGG AGTACATCAG
            601 TAAGATGGGC TACCTGGACC CCAGGAATGA GCAGATTGTG CGAGTCATCA AGCAGACCTC
            661 CCCAGGAAAG GGCCTGGCGC CCACCCCGGT GACTCGGCGG CCCAGTTTCG AGGGCACAGG
            721 GGAAGCACTC CCATCCTACC ACCAGCTGGG TGGTGCAAAC TACGAGGGCC CCGCCGCACT
            781 GGAGGAGATG CCGCGGCAAT ATTTAGACTT TCTCTTCCCT GGAGCCGGAG CCGGCACCCA
            841 CGGTGCCCAG GCTCACCAGC ATCCTCCCAA AGGGTACAGC ACAGCAGTAG AGCCAAGTGC
            901 GCACTTTCCG GGCACACACT ATGGGTCGTG GTCATCTACT ATCGGAGCAG CCTGGGTATG
            961 GGGTGCAGCC GCAGTTCCTC CCTTCCAGAA CAGGACGCCA CCAGATGCCT TATTTCCAGC
           1021 ATGGGCCAAG CCCCAAGGTG GGTCCCTCCC GCCAGCTCAC TTTTCCTGCC ATGCCTGGGC
           1081 TGTACACTGC CTCGCACCCC ACAAGCTGGC GGCTAACCCC ACCTTGGGGC CCACCCAATT
          1141 AACATGTATG TGTTTGGGGC AT
          //
          Primer: GNP_primer#10
          Sequence file: RDB12214_A6Ajb.seq check
          >12214_12214_A6Aj_pMAMneo_Reverse_E04_13.ab1
              1 TGTCAGTATG TCACACCACA GAAGTAAGGT TCCTTCACAA AGATCCTCTA GCATTTAGGT
             61 GACACTATAG AATAGGGCCC TCTAGATGCA TGCTCGAGTG CGGCCGCAAG CTTGTCGACG
            121 GAGCTCGAAT TCGGATCATC TGCAGAATTT CGAGGTTACA CGTACACCGG CTGGCAGCCC
            181 TCGGCCGCAC CTTCCAAGTC CGCATCCCCT GGGTCTGCAC TCTCTGCGGG CTCTGAGGGC
            241 TTCGGGCACC GGAAGGGATA GCCATTGTCA TCGAAGAACC TGCGGAAGGT GAACTCATAG
            301 AAGGCGTGCT CTGGATGCTT GCTGCTGGGG GAGGCCAGCG TGTCCCAGGC CTTGGCGCTC
            361 TCTCCGCTGG CCTCGTGCCA GGGGCTTTCT TCATCCACCG GGTCAAAATT GGAGGTGTCC
            421 ATGGGGTGGC TGATGGTGGG GACGTAGGGT GCAGGCTGCT TTCGGATGTC ACGGGAAAAG
            481 TCGATGGTGT TGAAGAACGG GTGTGCCTTG AGGTCATCTG CCCCATCCCT GCCCAGGCGG
            541 CAGTCAGCCG CGCAGCACAG CTTCGTGATG AGGTCTCGGG CCTCAGCGCT GAGCCTCACC
            601 TGCGTAGGGA TATGCAGCGT GCTCTCCCAG TTGATCACCT TCAGCTGCGT CTCTGTGGGG
            661 GTGGGGGCCA AGAAAGGCGG CTGCCCAACC AGCATCTCAA AGAGAATCAC ACCGACGCTC
            721 CACCAGTCAC AGAGCTGCGT GTACCCTTTG CGGAGAAGCA CCTCCGGAGC GATGTAATTT
            781 GGTGTCCCGA CAAGAGAATG TGCCAGGCAC CTCTGGTGCT GCTTCTGCGC CCTCTGCTCC
            841 AGGGTCTTTA ACCTGTCTCC ACAGCGACAG TTGGAAACAT CGTCCCAGAG GTCACCGGGC
            901 TCCATGCTGT CCTGTCTCAT GTGGTTCCCT TTCTGGTAGT ACTTGGAATT TGTGAGTCCA
            961 CCCTGAATCC AGTGCAGAGG CCAAAAGCTG TCAGCTAATA TGACCATCCA GGGTCGATGA
           1021 GTATGGTTGT CAGCTGAATG TCCCGATGAA TAAGGCCATC TGTGAACAAC TTTCATGGCC
          1081 AGGATCACTC TGCCAATGGT AAAAAAGCGG GACCAGAGAT
          //
          Primer: Lats2#11
          Sequence file: RDB12214_A6Ajc.seq check
          >12214_12214_A6Aj_Lats2#11_H07_22.ab1
              1 CAGAGGGCCA GCCGGGAGCT ACAGCTGGAG CAGGAAATGG CCAAAGCTGG GCTCTGTGAG
             61 GCCGAGCAGG AGCAGATGAG GAAGATCCTC TACCAGAAGG AGTCTAACTA CAACCGGCTG
            121 AAGAGGGCCA AGATGGACAA GTCCATGTTT GTGAAAATCA AGACTCTAGG CATCGGTGCC
            181 TTTGGGGAAG TGTGCCTCGC TTGTAAGCTG GACACTCACG CTCTGTACGC CATGAAGACT
            241 CTCAGGAAGA AGGATGTCCT GAACCGGAAT CAAGTGGCCC ATGTCAAGGC TGAGAGGGAC
            301 ATCCTGGCTG AAGCAGACAA TGAGTGGGTG GTCAAACTCT ACTACTCCTT CCAGGACAAG
            361 GACAGCCTGT ACTTTGTGAT GGACTACATA CCAGGCGGGG ATATGATGAG CCTGCTGATC
            421 AGGATGGAGG TCTTCCCTGA GCACCTGGCC CGCTTCTACA TTGCAGAGTT GACCCTGGCC
            481 ATTGAAAGTG TCCACAAGAT GGGCTTTATC CACCGGGACA TCAAGCCTGA CAACATACTC
            541 ATCGACCTGG ATGGTCATAT TAAGCTGACA GCTTTTGGCC TCTGCACTGG ATTCAGGTGG
            601 ACTCACAATT CCAAGTACTA CCAGAAAGGG AACCACATGA GACAGGACAG CATGGAGCCC
            661 GGTGACCTCT GGGACGATGT TTCCAACTGT CGCTGTGGAG ACAGGTTAAA GACCCTGGAG
            721 CAGAGGGCGC AGAAGCAGCA CCAGAGGTGC CTGGCACATT CTCTTGTCGG GACACCAAAT
            781 TACATCGCTC CGGAGGTGCT TCTCCGCAAA GGGGTACACG CAGCTCTGTG ACTGGTGGAG
            841 CGTCGGTGTG ATTCTCTTTG AGATGCTGGG TTGGGCAGCC GCCTTTCTTG GCCCCCACCC
            901 CCACAGAGAC GCAGCTGAAA GGTGATCAAC TGGGAGAGCA CGCTGCATAT CCCTACGCAG
            961 GTGAGGCTCA GCGCTGAGGC CCGAGACTCA TCACGAAGCT GTGCTGCGCG CTGACTGCCG
           1021 CCTGGGCAGG AATGGGGGCA GATGACCTCA AGGCACACCC GGTTCTCAAC ACATCGAACC
           1081 TATACCGTGA CATTCCGGAA GGCCAGGCCT GCACCCTTAC CGTTCGCAAC CCAATTTCAG
          1141 ATCGCA
          //
          Primer:
          Sequence file: RDB12214_A6Ajd.seq check
          1

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl