Resource data sheet
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pCMV/SV-Flag-Lats2-KD

Expression clone of mouse Lats2, kinase dead variant

Catalog number RDB12214
Resource name pCMV/SV-Flag-Lats2-KD
Clone info. Expression clone of mouse Lats2 cDNA. Catalytically inactive (kinase dead) variant. cDNA fragment 437 to 3,565 nt of NM_015771.2 (CDS, 437..3565) is cloned. A to C substitution at 2,736 nt (Asp to Ala at 767th aa, introduced mutation).
Known differences: C to T substitution at 3457 nt (Asn at 1007th aa, synonymous).
Vector backbone pCMV/SV-Flag1 (Plasmid)
Size of vector backbone 4.6 kb
Gene/insert name Mouse Lats2 cDNA
Depositor Sasaki, Hiroshi |
 
Other clones in our bank mouse Lats2 (NCBI Gene 50523) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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RDB12214 pCMV/SV-Flag-Lats2-KD DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12214_A6Ajp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB12214_A6Aja.seq
>12214_12214_A6Aj_CMV-Forward_A02_02.ab1
    1 AGTGGATTAA CCTCCCGGGC CCCATTTGAA CGCAATGGGC GGTAGGCGTG TACGGTGGGA
   61 GGTCTATATA AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA
  121 AATTAATACG ACTCACTATA GGGAGACCCA AGCTTCCACC ATGGACTACA AGGACGACGA
  181 TGACAAGGGA TCCATGAGGC CAAAGACTTT TCCTGCCACA ACTTACTCTG GAAATAGCCG
  241 GCAGCGATTG CAAGAGATTC GAGAGGGGCT GAAGCAGCCA TCCAAGGCTT CCACCCAGGG
  301 GCTGCTGGTG GGACCAAACA GTGACACTTC CCTGGATGCC AAAGTCCTGG GGAGCAAAGA
  361 TGCCTCCAGG CAGCAGCAAA TGAGAGCCAC CCCGAAGTTT GGACCTTATC AAAAAGCTCT
  421 CAGGGAAATC CGATATTCCC TCCTGCCTTT TGCCAACGAG TCAGGCACTT CGGCAGCTGC
  481 AGAGGTGAAC CGGCAGATGC TTCAGGAGTT GGTGAATGCG GGATGTGACC AGGAGATGGC
  541 TGGCAGAGCG CTCAAGCAGA CGGGCAGTAG GAGTATCGAA GCTGCCTTGG AGTACATCAG
  601 TAAGATGGGC TACCTGGACC CCAGGAATGA GCAGATTGTG CGAGTCATCA AGCAGACCTC
  661 CCCAGGAAAG GGCCTGGCGC CCACCCCGGT GACTCGGCGG CCCAGTTTCG AGGGCACAGG
  721 GGAAGCACTC CCATCCTACC ACCAGCTGGG TGGTGCAAAC TACGAGGGCC CCGCCGCACT
  781 GGAGGAGATG CCGCGGCAAT ATTTAGACTT TCTCTTCCCT GGAGCCGGAG CCGGCACCCA
  841 CGGTGCCCAG GCTCACCAGC ATCCTCCCAA AGGGTACAGC ACAGCAGTAG AGCCAAGTGC
  901 GCACTTTCCG GGCACACACT ATGGGTCGTG GTCATCTACT ATCGGAGCAG CCTGGGTATG
  961 GGGTGCAGCC GCAGTTCCTC CCTTCCAGAA CAGGACGCCA CCAGATGCCT TATTTCCAGC
 1021 ATGGGCCAAG CCCCAAGGTG GGTCCCTCCC GCCAGCTCAC TTTTCCTGCC ATGCCTGGGC
 1081 TGTACACTGC CTCGCACCCC ACAAGCTGGC GGCTAACCCC ACCTTGGGGC CCACCCAATT
1141 AACATGTATG TGTTTGGGGC AT
//
Primer: GNP_primer#10 RDB12214_A6Ajb.seq
>12214_12214_A6Aj_pMAMneo_Reverse_E04_13.ab1
    1 TGTCAGTATG TCACACCACA GAAGTAAGGT TCCTTCACAA AGATCCTCTA GCATTTAGGT
   61 GACACTATAG AATAGGGCCC TCTAGATGCA TGCTCGAGTG CGGCCGCAAG CTTGTCGACG
  121 GAGCTCGAAT TCGGATCATC TGCAGAATTT CGAGGTTACA CGTACACCGG CTGGCAGCCC
  181 TCGGCCGCAC CTTCCAAGTC CGCATCCCCT GGGTCTGCAC TCTCTGCGGG CTCTGAGGGC
  241 TTCGGGCACC GGAAGGGATA GCCATTGTCA TCGAAGAACC TGCGGAAGGT GAACTCATAG
  301 AAGGCGTGCT CTGGATGCTT GCTGCTGGGG GAGGCCAGCG TGTCCCAGGC CTTGGCGCTC
  361 TCTCCGCTGG CCTCGTGCCA GGGGCTTTCT TCATCCACCG GGTCAAAATT GGAGGTGTCC
  421 ATGGGGTGGC TGATGGTGGG GACGTAGGGT GCAGGCTGCT TTCGGATGTC ACGGGAAAAG
  481 TCGATGGTGT TGAAGAACGG GTGTGCCTTG AGGTCATCTG CCCCATCCCT GCCCAGGCGG
  541 CAGTCAGCCG CGCAGCACAG CTTCGTGATG AGGTCTCGGG CCTCAGCGCT GAGCCTCACC
  601 TGCGTAGGGA TATGCAGCGT GCTCTCCCAG TTGATCACCT TCAGCTGCGT CTCTGTGGGG
  661 GTGGGGGCCA AGAAAGGCGG CTGCCCAACC AGCATCTCAA AGAGAATCAC ACCGACGCTC
  721 CACCAGTCAC AGAGCTGCGT GTACCCTTTG CGGAGAAGCA CCTCCGGAGC GATGTAATTT
  781 GGTGTCCCGA CAAGAGAATG TGCCAGGCAC CTCTGGTGCT GCTTCTGCGC CCTCTGCTCC
  841 AGGGTCTTTA ACCTGTCTCC ACAGCGACAG TTGGAAACAT CGTCCCAGAG GTCACCGGGC
  901 TCCATGCTGT CCTGTCTCAT GTGGTTCCCT TTCTGGTAGT ACTTGGAATT TGTGAGTCCA
  961 CCCTGAATCC AGTGCAGAGG CCAAAAGCTG TCAGCTAATA TGACCATCCA GGGTCGATGA
 1021 GTATGGTTGT CAGCTGAATG TCCCGATGAA TAAGGCCATC TGTGAACAAC TTTCATGGCC
1081 AGGATCACTC TGCCAATGGT AAAAAAGCGG GACCAGAGAT
//
Primer: Lats2#11 RDB12214_A6Ajc.seq
>12214_12214_A6Aj_Lats2#11_H07_22.ab1
    1 CAGAGGGCCA GCCGGGAGCT ACAGCTGGAG CAGGAAATGG CCAAAGCTGG GCTCTGTGAG
   61 GCCGAGCAGG AGCAGATGAG GAAGATCCTC TACCAGAAGG AGTCTAACTA CAACCGGCTG
  121 AAGAGGGCCA AGATGGACAA GTCCATGTTT GTGAAAATCA AGACTCTAGG CATCGGTGCC
  181 TTTGGGGAAG TGTGCCTCGC TTGTAAGCTG GACACTCACG CTCTGTACGC CATGAAGACT
  241 CTCAGGAAGA AGGATGTCCT GAACCGGAAT CAAGTGGCCC ATGTCAAGGC TGAGAGGGAC
  301 ATCCTGGCTG AAGCAGACAA TGAGTGGGTG GTCAAACTCT ACTACTCCTT CCAGGACAAG
  361 GACAGCCTGT ACTTTGTGAT GGACTACATA CCAGGCGGGG ATATGATGAG CCTGCTGATC
  421 AGGATGGAGG TCTTCCCTGA GCACCTGGCC CGCTTCTACA TTGCAGAGTT GACCCTGGCC
  481 ATTGAAAGTG TCCACAAGAT GGGCTTTATC CACCGGGACA TCAAGCCTGA CAACATACTC
  541 ATCGACCTGG ATGGTCATAT TAAGCTGACA GCTTTTGGCC TCTGCACTGG ATTCAGGTGG
  601 ACTCACAATT CCAAGTACTA CCAGAAAGGG AACCACATGA GACAGGACAG CATGGAGCCC
  661 GGTGACCTCT GGGACGATGT TTCCAACTGT CGCTGTGGAG ACAGGTTAAA GACCCTGGAG
  721 CAGAGGGCGC AGAAGCAGCA CCAGAGGTGC CTGGCACATT CTCTTGTCGG GACACCAAAT
  781 TACATCGCTC CGGAGGTGCT TCTCCGCAAA GGGGTACACG CAGCTCTGTG ACTGGTGGAG
  841 CGTCGGTGTG ATTCTCTTTG AGATGCTGGG TTGGGCAGCC GCCTTTCTTG GCCCCCACCC
  901 CCACAGAGAC GCAGCTGAAA GGTGATCAAC TGGGAGAGCA CGCTGCATAT CCCTACGCAG
  961 GTGAGGCTCA GCGCTGAGGC CCGAGACTCA TCACGAAGCT GTGCTGCGCG CTGACTGCCG
 1021 CCTGGGCAGG AATGGGGGCA GATGACCTCA AGGCACACCC GGTTCTCAAC ACATCGAACC
 1081 TATACCGTGA CATTCCGGAA GGCCAGGCCT GCACCCTTAC CGTTCGCAAC CCAATTTCAG
1141 ATCGCA
//
Primer: RDB12214_A6Ajd.seq
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731.

2019.04.09

GNP_filter3_RDBDEP_html_190327.pl