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pCMV-Flag-Tead2

Expression clone of mouse Tead2, FLAG-tag

Catalog number RDB12171
Resource name pCMV-Flag-Tead2
Clone info. Expression clone of mouse Tead2, FLAG-tag.
Comment cDNA fragment 78 nt to 1,424 nt of NM_011565.2 is cloned. Known difference; G to A (Val to Ile) substitution at 453 nt.
Vector backbone pCMV/SV2 (Plasmid)
Size of vector backbone 4.6 kb
Gene/insert name Mouse Tead2 cDNA
Depositor|Developer Sasaki, Hiroshi |
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External Database
Mouse Tead2

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB12171 pCMV-Flag-Tead2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12171_A3I3p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,FLAG,insert 5'
          Sequence file: RDB12171_A3I3c.seq check
          >12171-3_CMV-Foward_B02_05.ab1
              1 NNNNNNNNNN NNNNNNNNNG NNNNNNNGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
             61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
            121 GACTCACTAT AGGGAGACCC AAGCTTCCAC CATGGACTAC AAGGACGACG ATGACAAGGG
            181 ATCCGAATTC GAGGCCCAGA TGGGGGATCC CCGGACTGGG GCCCCCCTGG ATGATGGCGG
            241 GGGCTGGACA GGTAGCGAGG AAGGCAGCGA AGAGGGCACC GGAGGCAGCG AGGGCGTTGG
            301 AGGTGATGGC AGCCCTGATG CAGAAGGTGT GTGGAGCCCC GACATTGAGC AGAGTTTTCA
            361 GGAGGCCCTG GCCATCTACC CTCCCTGTGG CCGTCGCAAG ATCATCCTGT CAGATGAGGG
            421 CAAGATGTAT GGCCGGAACG AACTCATTGC CCGTTACATC AAGCTGAGGA CAGGGAAGAC
            481 GAGAACGCGA AAGCAGGTCT CCAGCCATAT TCAGGTTTTG GCTCGAAGGA AATCGAGAGA
            541 AATTCAGTCC AAGCTGAAGG ACCAAATCTC CAAGGACAAG GCCTTCCAGA CGATGGCCAC
            601 CATGTCTTCG GCACAGCTCA TCTCCGCCCC TTCCCTCCAG GCCAAGCTGG GCCCTTCTGG
            661 CCCTCAGGCC ACTGAGCTTT TCCAGTTCTG GTCAGGGAGC TCTGGGCCAC CATGGAATGT
            721 TCCAGACGTG AAGCCCTTCT CACAGGCACC GTTCTCCGTG TCACTGACGC CCCCAGCCTC
            781 TGACCTACCA GGGTACGAGC CGCCCCCAGC CCTCTCACCC CTGCCCCCAC CCGCTCCGTC
            841 TCCCCCAGCC TGGCAGGCTC GGGCCCTGGG CNCTGCCCGC CTGCAGCTGA TAGANTTCTC
            901 AGCGTTTGTG GAANCGCCNG ANGCANTTGA NTCGNNNCNN ANGCNTCTGT TTGTCCNCNT
            961 CANTCAGCAN NGTCCCNNCC CNNNNNCANC ACCCCTANAN NGTGNGNNNG TGNNNNANNN
          1021 NTACNNNNAN NNNCNNNNNN NNNN
          //
          Primer: pMAMneo-Reverse
          Region: insert 3'
          Sequence file: RDB12171_A3I3d.seq check
          >12171-3_pMAMneo_Reverse_D10_10.ab1
              1 NNNNNNNNNN NNNNNNNNNN NCNNNANTAA GGNTCCTTCN CNAAGATCCT CTAGCATTTA
             61 GGTGACACTA TAGAATAGGG CCCTCTAGAT GCATGCTCGA GATCAGTCCC TGACCAGGCG
            121 GTAGATGTGG TACTGGGCTC CTCGTTCACT GGTGGAGACT TCAAAGACGT AGGCAGTACA
            181 CAGCAGCAGT TCCTGAGTGT CCCTGTTTGT CACAACCTGG AGGATGGTGA AGTTCTCCAG
            241 GACACTGTTC ATCATGTAGC GTTCAGGCAG CTGACGGAGC TTGTGCAGGA AATTAACCAG
            301 GTACTCACAC ATGGGAGAGC GCAGCAGACG GTACACAAAG CGCCCGTCCT CCAGCTGGGC
            361 CCGTTCCGTC TCCACCTTCT CTACCACTTG CTTGCCAAAG GAGCAGACCT TGGAGGAGCA
            421 GGTGAGTGTC ATGAGCTCCC GGCTCTCATA CTGGCTGCTC ACTCCATAGA AGCCACCGCC
            481 ACCTCCGCTG CTCCCTGCCT CCTCGGCACT GGGGCCCCAG TTCAGGTCCG CCCAGAACTT
            541 GACGAGGAAG AAGGCATGTG GTGGCCCTCG GTCATACAGC TCGCGGAGGC CGCCCTTCTT
            601 CTCAGGGAAT TTGTCGTAGA TCTGCCGCAC GTCCACNCTC TCTAGGGGTG GTGCTNNNNG
            661 GCTGGGACAC TGCTGACTGA TGTGGACAAA CAGATGCCTC TGGAACGAGT CAACTGCGTC
            721 TGGCGGTTCC ACNAAANGCT GANAACTCTA TCANCTGCAG GCGGGCAGTG CCCAGGGNCC
            781 GAGCCTGCCA GGCTGGGGGA NACGGANCGG GTGGGGGCAG GGGTGANAGG GCTGGGGGCG
            841 GNTCGTACCC TGGTAGGTCA NNNGCTGGGG GCGTCAGNGA CACNGNNNAA CGGTGCCTGT
            901 GANNNNGNNT TCACGNNTGG NACATTNCNA TGGNNNNCCN NANCTCCCTG ACCANAACTG
            961 NNAAAANNTC ANNNNCCTGA GGGCCANAAN GGCCCNNCTT NNNCNTGNAN GNANNGGGNN
           1021 GANNNNNNNN NGNGNCNNNA NNNNNNGNTG GNCNNTCNNN NNNNNNGNNC NNNNNCCNTN
          1081 NNNNNNTTNN GNTNNNNNNN NNN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori,AmpR
          Sequence file: RDB12171_A3I3e.seq check
          >12171_12171_B2EkF_SV40pro_ori_F_H04_22_ABI24.ab1
              1 NNNNNNNNNN NNNNNGNCGC CTCGNCCTCT GANCTATTCN NGAAGTAGTG AGGAGGCTTT
             61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTG ATCCTGGCGT AATAGCGAAG AGGCCCGCAC
            121 CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCCTGA TGCGGTATTT
            181 TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATGG TGCACTCTCA GTACAATCTG
            241 CTCTGATGCC GCATAGTTAA GCCAGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG
            301 ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG
            361 CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGACGAAAGG GCCTCGTGAT
            421 ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC
            481 TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT
            541 GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG
            601 TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC
            661 TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC
            721 ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC
            781 CGAANAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC
            841 NNGTATTGAC GCCGGGCAAG ANCAACTCGG TCGCCGCATA CACTATTCTC NNANNGACTN
            901 GNTTGANTAC TCACCNGTCA CAGAAAANCA TCTTACGGAN GGNATGACAG TAANANAATT
            961 ANGCANNGNT GCCNTAACNN TGANNGATNN NNCNGNNNCC NNNNNANTTN NGANNNNGAT
          1021 CNGNANNNNC NNNNNNNNTN NNNNNTTTTT TNNNNNNNNN N
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

          2022.06.07

          GNP_filter3_RDBDEP_html_220520.pl