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pCMV-Flag-Tead2

Expression clone of mouse Tead2, FLAG-tag

Catalog number RDB12171
Resource name pCMV-Flag-Tead2
Clone info. Expression clone of mouse Tead2, FLAG-tag.
cDNA fragment 78 nt to 1,424 nt of NM_011565.2 is cloned. Known difference; G to A (Val to Ile) substitution at 453 nt.
Vector backbone pCMV/SV2 (Plasmid)
Size of vector backbone 4.6 kb
Gene/insert name Mouse Tead2 cDNA
Depositor Sasaki, Hiroshi |
 
Other clones in our bank mouse Tead2 (NCBI Gene 21677) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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RDB12171 pCMV-Flag-Tead2 DNA solution

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References and tips

Electronic file

Featured content

References

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12171_A5L1p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB12171_A5L1a.seq
>12171_12171_A5L1_CMV-Forward_D09_12.ab1
    1 CTTTCACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTTCCACCA TGGACTACAA GGACGACGAT GACAAGGGAT
  181 CCGAATTCGA GGCCCAGATG GGGGATCCCC GGACTGGGGC CCCCCTGGAT GATGGCGGGG
  241 GCTGGACAGG TAGCGAGGAA GGCAGCGAAG AGGGCACCGG AGGCAGCGAG GGCGTTGGAG
  301 GTGATGGCAG CCCTGATGCA GAAGGTGTGT GGAGCCCCGA CATTGAGCAG AGTTTTCAGG
  361 AGGCCCTGGC CATCTACCCT CCCTGTGGCC GTCGCAAGAT CATCCTGTCA GATGAGGGCA
  421 AGATGTATGG CCGGAACGAA CTCATTGCCC GTTACATCAA GCTGAGGACA GGGAAGACGA
  481 GAACGCGAAA GCAGGTCTCC AGCCATATTC AGGTTTTGGC TCGAAGGAAA TCGAGAGAAA
  541 TTCAGTCCAA GCTGAAGGAC CAAATCTCCA AGGACAAGGC CTTCCAGACG ATGGCCACCA
  601 TGTCTTCGGC ACAGCTCATC TCCGCCCCTT CCCTCCAGGC CAAGCTGGGC CCTTCTGGCC
  661 CTCAGGCCAC TGAGCTTTTC CAGTTCTGGT CAGGGAGCTC TGGGCCACCA TGGAATGTTC
  721 CAGACGTGAA GCCCTTCTCA CAGGCACCGT TCTCCGTGTC ACTGACGCCC CCAGCCTCTG
  781 ACCTACCAGG GTACGAGCCG CCCCCAGCCC TCTCACCCCT GCCCCCACCC GCTCCGTCTC
  841 CCCCAGCCTG GCAGGCTCGG GCCCTGGGCA CTGCCCGCCT GCAGCTGATA GAGTTCTCAG
  901 CGTTTGTGGA ACCGCCAGAC GCAGTTGACT CGTTCCAGAG GCATCTGTTT GTCCACATCA
  961 GTCAGCAGTG TCCCAGCCCT GGAGCACCAC CCCTTAGAGA GTGTGGACGT GCGGGCAGAT
 1021 CTACGACAAA TTCCCTGAGA AGAAGGGCGG GCCTCCGCGG AGCTGTATGA CCCGAGGGGC
 1081 CACCCACATT GCCGTTCCTT CGTCGATCCA AGATCTGGGA CGGAACCTGG AACCTTGGGG
1141 GGCCCCAAGA TC
//
Primer: SV40-3'UTR RDB12171_A5L1b.seq
>12171_12171_A5L1_SV40-3'UTR_E09_15.ab1
    1 TTAAAACAGG TAGGTTCTTC ACAAAGATCC TCTAGCATTT AGGTGACACT ATAGAATAGG
   61 GCCCTCTAGA TGCATGCTCG AGATCAGTCC CTGACCAGGC GGTAGATGTG GTACTGGGCT
  121 CCTCGTTCAC TGGTGGAGAC TTCAAAGACG TAGGCAGTAC ACAGCAGCAG TTCCTGAGTG
  181 TCCCTGTTTG TCACAACCTG GAGGATGGTG AAGTTCTCCA GGACACTGTT CATCATGTAG
  241 CGTTCAGGCA GCTGACGGAG CTTGTGCAGG AAATTAACCA GGTACTCACA CATGGGAGAG
  301 CGCAGCAGAC GGTACACAAA GCGCCCGTCC TCCAGCTGGG CCCGTTCCGT CTCCACCTTC
  361 TCTACCACTT GCTTGCCAAA GGAGCAGACC TTGGAGGAGC AGGTGAGTGT CATGAGCTCC
  421 CGGCTCTCAT ACTGGCTGCT CACTCCATAG AAGCCACCGC CACCTCCGCT GCTCCCTGCC
  481 TCCTCGGCAC TGGGGCCCCA GTTCAGGTCC GCCCAGAACT TGACGAGGAA GAAGGCATGT
  541 GGTGGCCCTC GGTCATACAG CTCGCGGAGG CCGCCCTTCT TCTCAGGGAA TTTGTCGTAG
  601 ATCTGCCGCA CGTCCACACT CTCTAGGGGT GGTGCTCCAG GGCTGGGACA CTGCTGACTG
  661 ATGTGGACAA ACAGATGCCT CTGGAACGAG TCAACTGCGT CTGGCGGTTC CACAAACGCT
  721 GAGAACTCTA TCAGCTGCAG GCGGGCAGTG CCCAGGGCCC GAGCCTGCCA GGCTGGGGGA
  781 GACGGAGCGG GTGGGGGCAG GGGTGAGAGG GCTGGGGGCG GCTCGTACCC TGGTAGGTCA
  841 GAGGCTGGGG GCGTCAGTGA CACGGAGAAC GGTGCCTGTG AGAAGGGCTT CACGTCTGGA
  901 ACATTCCATG GTGGCCCAGA GCTCCCTGAC CAGAACTGGA AAAGCTCAGT GGCCCTGAGG
  961 GCCAGAAGGG CCCAGCTTGG CCTGTAGGGA AAGGGGCGGG AGATGAGCTG TGCCGAAGAC
 1021 ATGGTGGGCC CATCGTCTGG GACGCCTTGT CCTTGGAGAT TTGGTTCCTT CAGCCTTGAC
 1081 CTGATTTTCT CTCGATTTCA TCGAAGTCAA AAACCTGAAT ATGGATGGGA GACTTGCCTT
1141 TTTCGG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

2019.04.09

GNP_filter3_RDBDEP_html_190327.pl