Resource data sheet
Prev.

pMYs-HA-Yap1 S112A-IRES-EGFP

Retroviral vector of mouse Yap1, S112A mutant

Catalog number RDB12181
Resource name pMYs-HA-Yap1 S112A-IRES-EGFP
Clone info. Retroviral vector of mouse Yap1, S112A mutation in Tead BD. cDNA fragment 210 to 1,625 nt of NM_009534.3 (CDS, 207..1625) is cloned. T to G substitution at 540 nt and C to A substitution at 542 nt (Ser to Ala at 112th aa, introduced mutation).
Transfection can be confirmed by GFP. See Fig. 3A of Ota and Sasaki, Development 135: 4059-4069 (2008). Known differences: G to C substitution at 212 nt (Glu to Asp at 2nd aa). C to A substitution at 477 nt (Arg at 91st aa, synonymous) and XhoI site insertion at the position.
Vector backbone pMYs-IRES-EGFP (Plasmid)
Size of vector backbone 6.2 kb
Selectable markers Amp^r
Gene/insert name Mouse Yap1 cDNA
Depositor Sasaki, Hiroshi |
 
Other clones in our bank mouse Yap1 (NCBI Gene 22601) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Additional terms and conditions for distribution Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
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Schedule A (GFP-A) [Link]
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Please use Schedule A (GFP-A) additionally.
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RDB12181 pMYs-HA-Yap1 S112A-IRES-EGFP DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12181_A6Ajp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: 5'Retro (pFB-5') RDB12181_A6Aja.seq
>12181_12181_A6Aj_5'Retro(pFB-5')_E11_14.ab1
    1 TCAGCTTCTG TTTCGCGGAC TGCTAGTTAT TAAGGATCCC AGTGTGGTGG TACGGGAATT
   61 CCTGCAGGGG GCTAGCGAAT TCGATTAATA CGACTCACTA TAGGGAGACC CAAGCTTGCC
  121 GCCACCATGT ACCCATACGA CGTTCCAGAT TACGCTGGTA CCGACCCCGC GCAACAGCCG
  181 CCGCCCCAGC CGGCCCCGCA AGGCCCCGCG CCGCCGTCCG TGTCTCCGGC CGGGACCCCC
  241 GCGGCCCCGC CCGCACCCCC GGCCGGCCAC CAGGTCGTGC ACGTCCGCGG GGACTCGGAG
  301 ACCGACTTGG AGGCGCTCTT CAATGCCGTC ATGAACCCCA AGACGGCCAA CGTGCCTCAG
  361 ACCGTGCCCA TGCGGCTTCG CAAGCTGCCC GACTCCTTCT TCAAGCCGCC TGAGCCCAAG
  421 TCCCACTCGA GACAGGCCAG TACTGATGCA GGTACTGCGG GAGCTCTGAC TCCACAGCAT
  481 GTTCGAGCTC ACGCATCTCC AGCCTCCCTG CAGCTGGGTG CCGTTTCTCC TGGGACACTC
  541 ACAGCCAGTG GCGTTGTCTC TGGCCCTGCC GCTGCCCCTG CAGCTCAGCA TCTCCGGCAG
  601 TCCTCCTTTG AGATCCCTGA TGATGTACCA CTGCCAGCAG GCTGGGAGAT GGCCAAGACA
  661 TCTTCTGGTC AAAGATACTT CTTAAATCAC AACGATCAGA CAACAACATG GCAGGACCCC
  721 CGGAAGGCCA TGCTTTCGCA ACTGAACGTT CCTGCGCCTG CCAGCCCAGC GGTGCCCCAG
  781 ACGCTGATGA ATTCTGCCTC AGGACCTCTT CCTGATGGAT GGGAGCAAGC CATGACTCAG
  841 ATGGAGAAGT TTACTACATA AACCATAAGA ACAAGACCAC ATCCTGGCTG GACCCAAAGG
  901 CTGGACCCTC GTTTTGCCAT GAACCAGAGG ATCACTCAGA GTGCTCCAGT GAAGCAGCCC
  961 CCCACCCTTT GGCTCCCAGA GCCCACAGGG AGGCGTCTGG GGTGGAGGCA GTTCACAGCA
 1021 GCAGCAAATA CAGCTGCAGC AGTTACGATG GAGATGGAGA GACTGCGGTT GGAACCACCA
 1081 GATTATTTCG CAAGACTAGC TCTGCCAGCC AGGTTGCTAC ACTGAGCAGG AATGGGAGGG
1141 ACTTC
//
Primer: IRES-R RDB12181_A6Ajb.seq
>12181_12181_A6Aj_IRES_R_E02_14.ab1
    1 GCAATGCGGG CTTCGGCAGT AACGTTAGGG GGGGGGGAGG GAGAGGGGGG GGGGGGGCGG
   61 AATTTCGTAG CGGCCGCGGC GCGCCGGCCC TCGAGGGATC CCTCACAGAC TCAGAGTGGC
  121 TCCCTGCAGC TCTATAACCA CGTGAGAAAG CTTTCTTTAT CTAGCTTGGT GGCAGCCAAC
  181 ACAGACTCCA CGTCCAAGAT TTCGGAACTC AGCGCTTCCT GCAGACTGGG CATCAGCTCC
  241 TCCCCTTCTA TGTTCATTGC ATCTCCTTCC AGTGTGCCAA GGTCCACATT TGTCCCAGGG
  301 AGGGCTTCCA GGTAGTCGGG GAAGCGGCTC TGCTGTGACG GCAGGGTGCT TTGGCTGATG
  361 GTGTCTCCTG TATCCATTTC ATCCACACTG TTGAGGAAGT CGTCTGGGGT CCGAGGGATG
  421 CTGTAGCTGC TCATGCTGAG GCCGCTGTCT GTGCTCTCAT CTCGAGAGTG ATAGGTGCCA
  481 CTGTTAAGAA AGGGATCGGA ACTATTGGTT GTCATTGTTC TCAATTCCTG AGACATCCCA
  541 GGAGAAGACA CTGCATTCGG AGTCCCTCCA TCCTGCTCCA GTGTAGGCAA CTGGCTGCGC
  601 AGAGCTAATT CCTGCCGAAA TAATTCCTGT TGTTTCAACC GCAGTCTCTC CTTCTCCATC
  661 TGTAACTGCT GCAGCTGTAT TTGCTGCTGC TGGTTGGGAA CTGCCCTCCA CCCCAGACGC
  721 CTCCCCTGTG GGCTCTGGGG AGGCCAAGGG GTGGGGGCCT GCCTTCACTG GAGCACTCTG
  781 AGTGATCCCT CTGGTTCATG GCAAAACCGA GGGTCCAGCC CTTGGGGTCC CAGCCAGGAA
  841 TGTGGGTCCT TGTTTCTTAT GGTTTTATGG AAATAAACTT TCTCCATCCT GAAGTCGGGG
  901 CTTTGCTCCC CCTCTATCAG AGAAGAGCTC CTAGAAAGGA AAAATTTCTC CGGCTTGGTG
  961 GGGGCCACCT GGGGGTGGTG AGCGCGCCGG GAGACATTTC TACTTTGTGT AAAAAAATGG
 1021 GTGCCTCTCT TGGGGGGGGC GCTCTCGCGC CATGGGATTG CTGTGGCCAT GGTTGGTGTG
 1081 ATGTATTATC AGGATATTAT CTCTTTCTTT GCCCCACAGA GGAATGGTCT CTCTATGGGG
 1141 CCCTATCTCT CTACCCCAAG GCCTCTTGGA CCTGTGGCGC CGAAGGGTGC GGTGTCTATC
1201 ATCCATCTAT CGACTAC
//
Primer: IRES-F RDB12181_A6Ajc.seq
>12181_12181_A6Aj_IRES-F_E09_15.ab1
    1 GGCAGGCCGT TTCATGTGTT TAGTCGAGGT TAAAAAAACG TCTAGGCCCC CCGAACCACG
   61 GGGACGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACAACCAT GGTGAGCAAG
  121 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC
  181 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC
  241 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC
  301 CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC
  361 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC
  421 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC
  481 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC
  541 AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG
  601 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG
  661 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC
  721 CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC
  781 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTAAAG CGGCCGGCCG
  841 CCAGCACAGT GGTCGACCGG GCCGCCAGCA CAGTGGTCGA TCGAGGGGGG GGCCCGCGAT
  901 TAGTCCAATT TGTTAAAGAC AGGATATCAG TGGTCCAGGC TCTAGTTTTG ACTCAACAAT
  961 ATCACCAGCT GAAGCCCTAT AGAGTACGAG CCCATAGATA GAATAAAAGA TTTTATTTAG
 1021 TCTCAAGAAA AAGGGGGGGA ATGAAGACCC CAACCTGTAG GTTTGGCCAA GCTAGCTAGT
 1081 AACGGCATTT TGCCAGGCCA TTGGAAATAC ATTAACTGGA GAAATAAGGA GGAAAGTTCC
1141 AGGAT
//
Primer: Amp_R RDB12181_A6Ajd.seq
>12181_12181_A6Aj_Amp_R_F09_18.ab1
    1 GTCATTTCAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGCATAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT
  181 GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAAAAGTT CAGATCAAGG TCAGGAACAG
  241 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT
  301 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA
  361 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC
  421 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG
  481 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC
  541 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ATTGACTGAG
  601 TCGCCCGGGT ACCCGTATTC CCAATAAAGC CTCTTGCTGT TTGCATCCGA ATCGTGGACT
  661 CGCTGATCCT TGGGAGGGTC TCCTCAGATT GATTGACTGC CCACCTCGGG GGTCTTTCAT
  721 TTGGAGGTTC CACCGAGATT TGGAGACCCC TGCCCAGGGA CCACCGACCC CCCCGCCGGG
  781 AGGTAAGCTG GCCAGCGGTC GTTTCGTGTC TGTCTCTGTC TTTGTGCGTG TTTGTGCCGG
  841 CATCTAATGT TTGCGCCTGC GTCTGTACTA GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC
  901 CCGTGGTGGA ACTGACGAGT TCGGAACACC CGGGCCGCAA CCCTGGGAGA CGTCCCAGGG
  961 ACTTCGGGGG CCGTTTTTGT GGCCCGACCT GAGTCCAAAA TCCCGATCGT TTTGACTCTT
 1021 TGGTGCACCC CCTATAGAGG ATTATTGTGA TCTGGTAGAG ACGAGACTAA AAAGTCGCTT
1081 CGTCTGATTT TTGCTTCGAT TTGGACCGAA GTC
//
Primer: pMFG_1 RDB12181_A6Aje.seq
>12181_12181_A6Aj_pMFG-1_G09_21.ab1
    1 GCATTGGGGG GAAAGGGCTG TTAGTAACTC TTGTCATGTC AGGGTCAGGG AAGTTTACAA
   61 GGGGCGGGGG TGCCCCATAT AAGATCTCAT ATGGCCATAT GGGGGCGCCT AGAGAAGGAG
  121 TGAGGGCTGG ATAAAGGGAG GATCGAGGCG GGGTCGAACG AGGAGGTTCA AGGGGGAGAG
  181 ACGGGGCGGA TGGAGGAAGA GGAGGCGGAG GCTTAGGGTG TACAAAGGGC TTGACCCAGG
  241 GAGGGGGGTC AAAAGCCAAG GCTTCCCAGG TCACGATGTA GGGGACCTGG TCTGGGTGTC
  301 CATGCGGGCC AGGTGAAAAG ACCTTGATCT TAACCTGGGT GATGAGGTCT CGGTTAAAGG
  361 TGCCGTCTCG CGGCCATCCG ACGTTAAAGG TTGGCCATTC TGCAGAGCAG AAGGTAACCC
  421 AACGTCTCTT CTTGACATCT ACCGACTGGT TGTGAGCGAT CCGCTCGACA TCTTTCCAGT
  481 GACCTAAGGT CAAACTTAAA GGAGTGGTAA CAGTCTGGCC CTAATTTTCA GACAAATACA
  541 GAAACACAGT CAGACAGAGA CAACACAGAA CGATGCTGCA GCAGACAAGA CGCGCGGCGC
  601 GGCTTCGGTC CCAAACCGAA AGCAAAATTC AGACGGAGCG GGAACTGTTT AGTTCTCGTC
  661 TCCTACCAGA ACCACATATC CCTCCTATTA GGGGGGTGCA CCAAAGAAGT CAAACGATCG
  721 GGATTTTGGA CTCAGTCGGG CCACAAAAAC GGCCCCGGAG TCCTGGGACG TCTCCAAGTG
  781 CGCGGTGTTC GACTCGTCAG TTCCACCACG GTCCGCCAAA TACAGAGCTA GTTAGCTTAA
  841 CTTAGTTACA GACGCAAGGC TAAACATAAA ATGGCGGCCA AACCGCTCAG AACGAACGAA
  901 CCGAACGACG CTGGCACTAA CCTCCGCGGG GATCCGGTCC TTGCAGGGTT CTAATCCTGG
961 ACTCCATAGA AAGACCTGAG TGGGCTCTAC TTCAAGACCT CAG
//
Primer: EGFP-C RDB12181_A6Ajf.seq
>12181_12181_A6Aj_EGFP_C_G02_20.ab1
    1 CGAAGGGTTA CTTTCGGGCA TGGACGAGCT GTACAAGTAA AGCGGCCGGC CGCCAGCACA
   61 GTGGTCGACC GGGCCGCCAG CACAGTGGTC GATCGAGGGG GGGCCCGCGA TTAGTCCAAT
  121 TTGTTAAAGA CAGGATATCA GTGGTCCAGG CTCTAGTTTT GACTCAACAA TATCACCAGC
  181 TGAAGCCTAT AGAGTACGAG CCATAGATAG AATAAAAGAT TTTATTTAGT CTCCAGAAAA
  241 AGGGGGGAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT
  301 GCAAGGCATG GAAAATACAT AACTGAGAAT AGAGAAGTTC AGATCAAGGT TAGGAACAGA
  361 GAGACAGCAG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA
  421 GGGCCAAGAA CAGTTGGAAC AGCAGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT
  481 TCCTGCCCCG GCTCAGGGCC AAGAACAGAT GGTCCCCAGA TGCGGTCCCG CCCTCAGCAG
  541 TTTCTAGAGA ACCATCAGAT GTTTCCAGGG TGCCCCAAGG ACCTGAAATG ACCCTGTGCC
  601 TTATTTGAAC TAACCAATCA GTTCGCTTCT CGCTTCTGTT CGCGCGCTTC TGCTCCCCGA
  661 GCTCAATAAA AAGAGCCCAC AACCCCTCAC TCGGCGCGCC AGTCCTCCGA TAGACTGCGT
  721 CGCCCGGGTA CCCGTGTATC CAATAAACCC TCTTGCAGTT GCATCCGACT TGTGGTCTCG
  781 CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA TTGACTACCC GTCAGCGGGG GTCTTTCACA
  841 TGCAGCATGT ATCAAATTAA TTTGGTTTTT TTTTCTTAAA GTATTTACAT TAAATGGCCC
  901 ATAGTTTGCA TTAATGAAAT CGGGCCAACG GCGCGGGGAG AGGCCGGTTT GCGTATTGGG
  961 CGCTCTTTCC GCTTCCTCGC TCCACTGACT CGCTGCGCCT CGGTCGTTCG GCCTGGCGGG
 1021 CGAGCCGTAT CAGCTTCAAC TCAAAAGGCG TAATACGGTT ATTCCACAGG AATTCAGGAA
1081 TACGCCAGGC AGGACCATGT GAGCCAAGGC CAGCCAAAGC CAGGAACGCG TATA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

2019.04.09

GNP_filter3_RDBDEP_html_190327.pl