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pMYs-Tead2-EnR-IRES-EGFP

Retroviral vector of mouse Tead2, dominant negative

Catalog number RDB12175
Resource name pMYs-Tead2-EnR-IRES-EGFP
Clone info. Retroviral vector of mouse Tead2, Engrailed repression domain, dominant negative.
Comment C-terminal Myc-tag. Transfection can be confirmed by GFP. See Fig. 2B of Ota and Sasaki, Development 135: 4059-4069 (2008).
Vector backbone pMYs-IRES-EGFP (Plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r
Gene/insert name Mouse Tead2 cDNA Drosophila engrailed cDNA
Depositor|Developer Sasaki, Hiroshi |
Other clones in our bank

External Database
Mouse Tead2 Drosophila engrailed

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
        Additional terms and conditions for distribution Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
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        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
        Remarks Remember that you will be working with samples containing infections virus.
        提供案内 (日本国内) [open/close]

        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
        付加的提供条件 pMXsシリーズベクターについて:利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄博士への謝辞の表明を必要とする。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
        備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB12175 pMYs-Tead2-EnR-IRES-EGFP DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

        Further references such as user reports and related articles (go to bottom)

        Featured content


        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB12175_A6Ajp1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: 5'Retro (pFB-5')
        Sequence file: RDB12175_A6Aja.seq check
        >12175_12175_A6Aj_5'Retro(pFB-5')_G07_19.ab1
            1 CTGTCAGTCG CTGCATCATA TCGGACTGCT AGTTATTAAG GATCCGCCGC CATGGCTCCA
           61 AAGAAGAAGC GTAAGGTAAA TCGATCGAAT TCTGATGCAG AAGGTGTGTG GAGCCCCGAC
          121 ATTGAGCAGA GTTTTCAGGA GGCCCTGGCC ATCTACCCTC CCTGTGGCCG TCGCAAGATC
          181 ATCCTGTCAG ATGAGGGCAA GATGTATGGC CGGAACGAAC TCATTGCCCG TTACATCAAG
          241 CTGAGGACAG GGAAGACGAG AACGCGAAAG CAGGTCTCCA GCCATATTCA GGTTTTGGCT
          301 CGAAGGAAAT CGAGAGAAAT TCAGTCCAAG CTGAAGGACC AAGTCTCCAT CGATGCCCTG
          361 GAGGATCGCT GCAGCCCACA GTCAGCGCCC AGCCCCATTA CCCTACAAAT GCAGCATCTT
          421 CACCACCAGC AACAGCAGCA GCAGCAACAG CAGCAGCAAA TGCAGCACCT CCACCAGCTG
          481 CAGCAACTGC AGCAGTTGCA CCAACAGCAA CTGGCCGCCG GTGTCTTCCA CCATCCGGCA
          541 ATGGCCTTCG ATGCCGCTGC AGCCGCCGCT GCTGCAGCTG CTGCTGCGGC CGCCCACGCT
          601 CATGCTGCTG CACTGCAGCA GCGCCTCAGT GGCAGTGGAT CGCCCGCATC CTGCTCCACG
          661 CCCGCCTCGT CCACGCCGCT GACCATCAAG GAGGAGGAAA GCGACTCCGT GATCGGTGAC
          721 ATGAGTTTCC ACAATCAGAC GCACACCACC AACGAGGAGG AGGAGGCGGA GGAGGATGAC
          781 GACATTGATG TGGATGTGGA TGATACGTCG GCGGGCGGAC GCCTGCCACC ACCCGCCCAC
          841 CAGCAGCAGT CGACGGCCAA GCCCTCGCTG GCCTTTTCCA TCTCCCAACA TCCTGAGCGA
          901 TCGTTTCGGA GATGTCCAGA AGCCTGGCAA GTCGATTGAG AACCAGGGCC AGCATATTCC
          961 CGCCCCTTTC GAGGCGAATC GCTCCCAGAC TGCCACGCCC TCCGCCTTTA CAGAGTGCAT
         1021 CTGCTGGGAG TTAGCCGGCC ACCAGCCAAG CTGCGGCGCA GCCGCTACTG CCGTCATGAT
        1081 GCCTGGACGG TCAACTTCCT ACTGCTCATC CGCCTGGCTA TCCCAAGGAT TACACG
        //
        Primer: IRES-R
        Sequence file: RDB12175_A6Ajb.seq check
        >12175_12175_A6Aj_IRES-R_B08_05.ab1
            1 GGCTAGCGGC TTCGGCAGTA CGTTAGGGGG GGGGGAGGGA GAGGGGGGGG GGGGGCGGAA
           61 TTTACGTAGC GGCCGCGGCG CGCCGGCCCT CGAGGCCTGC AGGAATTCCC GTACCACCAC
          121 ACTGGGATCC CTAGTTCAGG TCCTCCTCGG AGATCAGCTT CTGCTCAAGC TTGGGCTGCA
          181 TAGATCCCAG AGCAGATTTC TCTGGATTGG CATCGCTCAT CTTGGAGCTC ATGGGCGGCG
          241 GGATGACGGC ATTGGCTGCA CTCCTGCGCA GGCGACTCTG CACGATTTCC TCGTGTATCC
          301 TGGGATAGGC AGCCGGATTG AAGCAGTTAA GGAAGTTGGC CCGTTCCAGC ATCATGGCCG
          361 CAGTAGCGGC TGCGGCGGCA GCTTGCTGTT GCCGGCTAAA CTCCAGCAGA TCCACTCTTG
          421 TAAAGGCGGA GGGCGTGGCA GTCTGGGAGC GATTCGCCTC GAAGGGGCGG AATATGCTGG
          481 CCTGGTTCTC AATCGACTTG CCAGGCTTCT GGACATCTCC GAAACGATCG CTCAGATGTT
          541 GGAGATGGAA AAGGCCAGCG AGGGCTTGGC CCGTCGACTG CTGCTGGTGG GCGGGTGGTG
          601 GCAGCGTCCG CCCGCCGACG TATCATCCAC ATCACATCAA TGTCGTCATC TCCTCGCTCT
          661 CTCCTCGTGG TGTGTGCGTC TGATTGTGGA AACTCATGTC ACCGATCACG GAGTCCGCTT
          721 CCTCCTCCTG ATGTCAACGG CGTGGACAAG CGGCTGACCA GATGCGGGCA TCCACTGCCA
          781 CTGAGCCTGC TTGCATGCAA CAACATGAAC CGTGGGGGCG CAAAACACTT GCAGACGGAG
          841 CTGCACGGAT GAAGGCATTG CGGATGTGAA ACTCCGCCGC AATTGTCTGT GTTGTGTCCA
        901 CACTCGTGTC GCATGTCCTG CGCACACATG TGTAAGGTCT CGCAATTCGT CACGTG
        //
        Primer: IRES-F
        Sequence file: RDB12175_A6Ajc.seq check
        >12175_12175_A6Aj_IRES-F_H07_22.ab1
            1 CTCCGTTGCG TTTAAATGTG GTTTAGTCGA GGTTAAAAAA CGTCTAGGCC CCCCGAACCA
           61 CGGGGACGTG GTTTTCCTTT GAAAAACACG ATGATAATAT GGCCACAACC ATGGTGAGCA
          121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
          181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
          241 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
          301 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT
          361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
          421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
          481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
          541 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG
          601 TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC
          661 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA
          721 CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT
          781 TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAA AGCGGCCGGC
          841 CGCCAGCACA GTGGTCGACC GGGCCGCCAG CACAGTGGTC GATCGAGGGG GGGGCCCGCG
          901 ATTAGTCCAA TTTGTTAAAG GACAGGATAT CAGTGGGTCC AGGCTCTAGT TTTGACTCAA
          961 CAATATCACC AGCTGAAGCC TATAGAGTAC GAGCCATAGA TAGAATAAAA GGATTTTAAT
         1021 TTAGTCTCCA GAAAAAGGGG GGGAAATGAA GGACCCCCAC CTGTAGGTTT GGCCAAGCTT
         1081 AGCTTAGTAA CGCCATTTTT GGCAAGGCCA TGAAAATACA TAACCTTGAG AATGAGGAAA
        1141 GTTCCAGGAT TCAAGGGGTT AAG
        //
        Primer: Amp_R
        Sequence file: RDB12175_A6Ajd.seq check
        >12175_12175_A6Aj_Amp_R_A08_02.ab1
            1 TTCGTTTCAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
           61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
          121 CCTGCATAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT
          181 GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAAAAGTT CAGATCAAGG TCAGGAACAG
          241 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT
          301 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA
          361 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC
          421 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG
          481 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC
          541 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ATTGACTGAG
          601 TCGCCCGGGT ACCCGTATTC CCAATAAAGC CTCTTGCTGT TTGCATCCGA ATCGTGGACT
          661 CGCTGATCCT TGGGAGGGTC TCCTCAGATT GATTGACTGC CCACCTCGGG GGTCTTTCAT
          721 TTGGAGGTTC CACCGAGATT TGGAGACCCC TGCCCAGGGA CCACCGACCC CCCCGCCGGG
          781 AGGTAAGCTG GCCAGCGGTC GTTTCGTGTC TGTCTCTGTC TTTGTGCGTG TTTGTGCCGG
          841 CATCTAATGT TTGCGCCTGC GTCTGTACTA GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC
          901 CCGTGGTGGA ACTGACGAGT TCGGAACACC CGGCCGCAAC CCTGGGAGAC GTCCCACGGA
          961 CTTCGGGGGG CCGTTTTTGT GGCCGACCTG AGTCCAAAAA TCCCGATCGT TTTGGACTCT
         1021 TTGGTGCACC CCCCCTAATA GGAGGATATG TGGATCTGGT AGGAGACGAC ACTTAAAACA
         1081 GGTCCGCTCG TCTGGAATTT TGGCTTCGCT GGACGAGCGG TCGACGACTG ATCGCTGCAG
        1141 CACTTCGGA
        //
        Primer: pMFG_1
        Sequence file: RDB12175_A6Aje.seq check
        >12175_12175_A6Aj_pMFG-1_B08_05.ab1
            1 GAATGGGGGA GGGCTGTTAG TAACTCTTGT CATGTCAGGG TCAGGGAAGT TTACAAGGGG
           61 CGGGGGTGCC CCATATAAGA TCTCATATGG CCATATGGGG GCGCCTAGAG AAGGAGTGAG
          121 GGCTGGATAA AGGGAGGATC GAGGCGGGGT CGAACGAGGA GGTTCAAGGG GGAGAGACGG
          181 GGCGGATGGA GGAAGAGGAG GCGGAGGCTT AGGGTGTACA AAGGGCTTGA CCCAGGGAGG
          241 GGGGTCAAAA GCCAAGGCTT CCCAGGTCAC GATGTAGGGG ACCTGGTCTG GGTGTCCATG
          301 CGGGCCAGGT GAAAAGACCT TGATCTTAAC CTGGGTGATG AGGTCTCGGT TAAAGGTGCC
          361 GTCTCGCGGC CATCCGACGT TAAAGGTTGG CCATTCTGCA GAGCAGAAGG TAACCCAACG
          421 TCTCTTCTTG ACATCTACCG ACTGGTTGTG AGCGATCCGC TCGACATCTT TCCAGTGACC
          481 TAAGGTCAAA CTTAAGGGAG TGGTAACAGT CTGGCCCTAA TTTTCAGACA AATACAGAAA
          541 CACAGTCAGA CAGAGACAAC ACAGAACGAT GCTGCAGCAG ACAAGACGCG CGGCGCGGCT
          601 TCGGTCCCAA ACCGAAAGCA AAAATTCAGA CGGAGGCGGG AACTGTTTTA GGTTCTCGTC
          661 TCCTACCAGA ACCACATATC CCTCCTATTA GGGGGGTGCA CCAAAGAGTC CAAAACGATC
          721 GGGATTTTTG GACTCAGGTC GGGCCACAAA AACGGCCCCC GAAGTCCCTG GGACGTCTCC
          781 CAGGGTTGCG GCCGGGTGTT CCGAACTCGT CAGTTCCACC ACGGGTCCGC CAGATACAGA
          841 GCTAGTTAGC TAACTAGTAC AGACGCACGC GCAAACATTA GATGCCGGCA CAAACACGCA
          901 CAAAGACAGA GACAGACACG AAACGACCGC TGGGCCAGCT TACCTCCCGG CGGGGGGGTC
          961 GGTGGTCCCT GGGCAGGGGT CTCCAATCTC GGTGGAACCT CCAAATGAAA GACCCCCGAG
         1021 GTGGGCAGTC AATCAATCTG AGGAGACCCT CCAGATCAGC GGAGTCACGA TCCGGATGCC
        1081 AAACAGCCAG AGGCTTATGG ATACGTTACC GGCGACTCAG TTCAATCGGA GGTACTTGC
        //
        Primer: EGFP-C
        Sequence file: RDB12175_A6Ajf.seq check
        >12175_12175_A6Aj_EGFP-C_C08_08.ab1
            1 CCACGGGATC TCTCGGGGCA TGGGACGAGC TGTACAAGTA AAGCGGCCGG CCGCCAGCAC
           61 AGTGGTCGAC CGGGCCGCCA GCACAGTGGT CGATCGAGGG GGGGCCCGCG ATTAGTCCAA
          121 TTTGTTAAAG ACAGGATATC AGTGGTCCAG GCTCTAGTTT TGACTCAACA ATATCACCAG
          181 CTGAAGCCTA TAGAGTACGA GCCATAGATA GAATAAAAGA TTTTATTTAG TCTCCAGAAA
          241 AAGGGGGGAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT
          301 TGCAAGGCAT GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG
          361 AGAGACAGCA GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC
          421 AGGGCCAAGA ACAGTTGGAA CAGCAGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG
          481 TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGTCCCCAG ATGCGGTCCC GCCCTCAGCA
          541 GTTTCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG GACCTGAAAT GACCCTGTGC
          601 CTTATTTGAA CTAACCAATC AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG
          661 AGCTCAATAA AAGAGCCCAC AACCCCTCAC TCGGCGCGCC AGTCCTCCGA TAGACTGCGT
          721 CGCCCGGGTA CCCGTGTATC CAATAAACCC TCTTGCAGTT GCATCCGACT TGTGGTCTCG
          781 CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA TTGACTACCC GTCAGCGGGG GTCTTTCACA
          841 TGCAGCATGT ATCAAAATTA ATTTGGTTTT TTTTTCTTAA GTATTTACAT TAAATGGCCA
          901 TAGTTGCATT AAATGAATCG GCCAACGCGC GGGGAGAGGC GGGTTTGCGT ATTGGGCGCT
          961 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT
         1021 CAGCTCACTC AAGGCGGTAA TACGGTTATC CACAGGAATC AGGGGATAAC GGCAGAAGAA
        1081 CATGTGAAGC TAAGGCCAGC AAGCCAGAAA TCGTAAAAAG GCGCGCGATT GGCTT
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

        2022.07.13

        GNP_filter3_RDBDEP_html_220713.pl