Resource data sheet
Prev.

pMYs-Tead2-EnR-IRES-EGFP

Retroviral vector of mouse Tead2, dominant negative

Catalog number RDB12175
Resource name pMYs-Tead2-EnR-IRES-EGFP
Clone info. Retroviral vector of mouse Tead2, Engrailed repression domain, dominant negative.
C-terminal Myc-tag. Transfection can be confirmed by GFP. See Fig. 2B of Ota and Sasaki, Development 135: 4059-4069 (2008).
Vector backbone pMYs-IRES-EGFP (Plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r
Gene/insert name Mouse Tead2 cDNA Drosophila engrailed cDNA
Depositor Sasaki, Hiroshi |
 
Other clones in our bank mouse Tead2 (NCBI Gene 21677) | Drosophila en (KEGG orthology K09319) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Additional terms and conditions for distribution Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
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Schedule A (GFP-A) [Link]
Remarks Remember that you will be working with samples containing infections virus.
Please use Schedule A (GFP-A) additionally.
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RDB12175 pMYs-Tead2-EnR-IRES-EGFP DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12175_A6Ajp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: 5'Retro (pFB-5') RDB12175_A6Aja.seq
>12175_12175_A6Aj_5'Retro(pFB-5')_G07_19.ab1
    1 CTGTCAGTCG CTGCATCATA TCGGACTGCT AGTTATTAAG GATCCGCCGC CATGGCTCCA
   61 AAGAAGAAGC GTAAGGTAAA TCGATCGAAT TCTGATGCAG AAGGTGTGTG GAGCCCCGAC
  121 ATTGAGCAGA GTTTTCAGGA GGCCCTGGCC ATCTACCCTC CCTGTGGCCG TCGCAAGATC
  181 ATCCTGTCAG ATGAGGGCAA GATGTATGGC CGGAACGAAC TCATTGCCCG TTACATCAAG
  241 CTGAGGACAG GGAAGACGAG AACGCGAAAG CAGGTCTCCA GCCATATTCA GGTTTTGGCT
  301 CGAAGGAAAT CGAGAGAAAT TCAGTCCAAG CTGAAGGACC AAGTCTCCAT CGATGCCCTG
  361 GAGGATCGCT GCAGCCCACA GTCAGCGCCC AGCCCCATTA CCCTACAAAT GCAGCATCTT
  421 CACCACCAGC AACAGCAGCA GCAGCAACAG CAGCAGCAAA TGCAGCACCT CCACCAGCTG
  481 CAGCAACTGC AGCAGTTGCA CCAACAGCAA CTGGCCGCCG GTGTCTTCCA CCATCCGGCA
  541 ATGGCCTTCG ATGCCGCTGC AGCCGCCGCT GCTGCAGCTG CTGCTGCGGC CGCCCACGCT
  601 CATGCTGCTG CACTGCAGCA GCGCCTCAGT GGCAGTGGAT CGCCCGCATC CTGCTCCACG
  661 CCCGCCTCGT CCACGCCGCT GACCATCAAG GAGGAGGAAA GCGACTCCGT GATCGGTGAC
  721 ATGAGTTTCC ACAATCAGAC GCACACCACC AACGAGGAGG AGGAGGCGGA GGAGGATGAC
  781 GACATTGATG TGGATGTGGA TGATACGTCG GCGGGCGGAC GCCTGCCACC ACCCGCCCAC
  841 CAGCAGCAGT CGACGGCCAA GCCCTCGCTG GCCTTTTCCA TCTCCCAACA TCCTGAGCGA
  901 TCGTTTCGGA GATGTCCAGA AGCCTGGCAA GTCGATTGAG AACCAGGGCC AGCATATTCC
  961 CGCCCCTTTC GAGGCGAATC GCTCCCAGAC TGCCACGCCC TCCGCCTTTA CAGAGTGCAT
 1021 CTGCTGGGAG TTAGCCGGCC ACCAGCCAAG CTGCGGCGCA GCCGCTACTG CCGTCATGAT
1081 GCCTGGACGG TCAACTTCCT ACTGCTCATC CGCCTGGCTA TCCCAAGGAT TACACG
//
Primer: IRES-R RDB12175_A6Ajb.seq
>12175_12175_A6Aj_IRES-R_B08_05.ab1
    1 GGCTAGCGGC TTCGGCAGTA CGTTAGGGGG GGGGGAGGGA GAGGGGGGGG GGGGGCGGAA
   61 TTTACGTAGC GGCCGCGGCG CGCCGGCCCT CGAGGCCTGC AGGAATTCCC GTACCACCAC
  121 ACTGGGATCC CTAGTTCAGG TCCTCCTCGG AGATCAGCTT CTGCTCAAGC TTGGGCTGCA
  181 TAGATCCCAG AGCAGATTTC TCTGGATTGG CATCGCTCAT CTTGGAGCTC ATGGGCGGCG
  241 GGATGACGGC ATTGGCTGCA CTCCTGCGCA GGCGACTCTG CACGATTTCC TCGTGTATCC
  301 TGGGATAGGC AGCCGGATTG AAGCAGTTAA GGAAGTTGGC CCGTTCCAGC ATCATGGCCG
  361 CAGTAGCGGC TGCGGCGGCA GCTTGCTGTT GCCGGCTAAA CTCCAGCAGA TCCACTCTTG
  421 TAAAGGCGGA GGGCGTGGCA GTCTGGGAGC GATTCGCCTC GAAGGGGCGG AATATGCTGG
  481 CCTGGTTCTC AATCGACTTG CCAGGCTTCT GGACATCTCC GAAACGATCG CTCAGATGTT
  541 GGAGATGGAA AAGGCCAGCG AGGGCTTGGC CCGTCGACTG CTGCTGGTGG GCGGGTGGTG
  601 GCAGCGTCCG CCCGCCGACG TATCATCCAC ATCACATCAA TGTCGTCATC TCCTCGCTCT
  661 CTCCTCGTGG TGTGTGCGTC TGATTGTGGA AACTCATGTC ACCGATCACG GAGTCCGCTT
  721 CCTCCTCCTG ATGTCAACGG CGTGGACAAG CGGCTGACCA GATGCGGGCA TCCACTGCCA
  781 CTGAGCCTGC TTGCATGCAA CAACATGAAC CGTGGGGGCG CAAAACACTT GCAGACGGAG
  841 CTGCACGGAT GAAGGCATTG CGGATGTGAA ACTCCGCCGC AATTGTCTGT GTTGTGTCCA
901 CACTCGTGTC GCATGTCCTG CGCACACATG TGTAAGGTCT CGCAATTCGT CACGTG
//
Primer: IRES-F RDB12175_A6Ajc.seq
>12175_12175_A6Aj_IRES-F_H07_22.ab1
    1 CTCCGTTGCG TTTAAATGTG GTTTAGTCGA GGTTAAAAAA CGTCTAGGCC CCCCGAACCA
   61 CGGGGACGTG GTTTTCCTTT GAAAAACACG ATGATAATAT GGCCACAACC ATGGTGAGCA
  121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
  181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
  241 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
  301 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT
  361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
  421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
  481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
  541 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG
  601 TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC
  661 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA
  721 CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT
  781 TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAA AGCGGCCGGC
  841 CGCCAGCACA GTGGTCGACC GGGCCGCCAG CACAGTGGTC GATCGAGGGG GGGGCCCGCG
  901 ATTAGTCCAA TTTGTTAAAG GACAGGATAT CAGTGGGTCC AGGCTCTAGT TTTGACTCAA
  961 CAATATCACC AGCTGAAGCC TATAGAGTAC GAGCCATAGA TAGAATAAAA GGATTTTAAT
 1021 TTAGTCTCCA GAAAAAGGGG GGGAAATGAA GGACCCCCAC CTGTAGGTTT GGCCAAGCTT
 1081 AGCTTAGTAA CGCCATTTTT GGCAAGGCCA TGAAAATACA TAACCTTGAG AATGAGGAAA
1141 GTTCCAGGAT TCAAGGGGTT AAG
//
Primer: Amp_R RDB12175_A6Ajd.seq
>12175_12175_A6Aj_Amp_R_A08_02.ab1
    1 TTCGTTTCAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGCATAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT
  181 GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAAAAGTT CAGATCAAGG TCAGGAACAG
  241 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT
  301 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA
  361 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC
  421 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG
  481 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC
  541 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ATTGACTGAG
  601 TCGCCCGGGT ACCCGTATTC CCAATAAAGC CTCTTGCTGT TTGCATCCGA ATCGTGGACT
  661 CGCTGATCCT TGGGAGGGTC TCCTCAGATT GATTGACTGC CCACCTCGGG GGTCTTTCAT
  721 TTGGAGGTTC CACCGAGATT TGGAGACCCC TGCCCAGGGA CCACCGACCC CCCCGCCGGG
  781 AGGTAAGCTG GCCAGCGGTC GTTTCGTGTC TGTCTCTGTC TTTGTGCGTG TTTGTGCCGG
  841 CATCTAATGT TTGCGCCTGC GTCTGTACTA GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC
  901 CCGTGGTGGA ACTGACGAGT TCGGAACACC CGGCCGCAAC CCTGGGAGAC GTCCCACGGA
  961 CTTCGGGGGG CCGTTTTTGT GGCCGACCTG AGTCCAAAAA TCCCGATCGT TTTGGACTCT
 1021 TTGGTGCACC CCCCCTAATA GGAGGATATG TGGATCTGGT AGGAGACGAC ACTTAAAACA
 1081 GGTCCGCTCG TCTGGAATTT TGGCTTCGCT GGACGAGCGG TCGACGACTG ATCGCTGCAG
1141 CACTTCGGA
//
Primer: pMFG_1 RDB12175_A6Aje.seq
>12175_12175_A6Aj_pMFG-1_B08_05.ab1
    1 GAATGGGGGA GGGCTGTTAG TAACTCTTGT CATGTCAGGG TCAGGGAAGT TTACAAGGGG
   61 CGGGGGTGCC CCATATAAGA TCTCATATGG CCATATGGGG GCGCCTAGAG AAGGAGTGAG
  121 GGCTGGATAA AGGGAGGATC GAGGCGGGGT CGAACGAGGA GGTTCAAGGG GGAGAGACGG
  181 GGCGGATGGA GGAAGAGGAG GCGGAGGCTT AGGGTGTACA AAGGGCTTGA CCCAGGGAGG
  241 GGGGTCAAAA GCCAAGGCTT CCCAGGTCAC GATGTAGGGG ACCTGGTCTG GGTGTCCATG
  301 CGGGCCAGGT GAAAAGACCT TGATCTTAAC CTGGGTGATG AGGTCTCGGT TAAAGGTGCC
  361 GTCTCGCGGC CATCCGACGT TAAAGGTTGG CCATTCTGCA GAGCAGAAGG TAACCCAACG
  421 TCTCTTCTTG ACATCTACCG ACTGGTTGTG AGCGATCCGC TCGACATCTT TCCAGTGACC
  481 TAAGGTCAAA CTTAAGGGAG TGGTAACAGT CTGGCCCTAA TTTTCAGACA AATACAGAAA
  541 CACAGTCAGA CAGAGACAAC ACAGAACGAT GCTGCAGCAG ACAAGACGCG CGGCGCGGCT
  601 TCGGTCCCAA ACCGAAAGCA AAAATTCAGA CGGAGGCGGG AACTGTTTTA GGTTCTCGTC
  661 TCCTACCAGA ACCACATATC CCTCCTATTA GGGGGGTGCA CCAAAGAGTC CAAAACGATC
  721 GGGATTTTTG GACTCAGGTC GGGCCACAAA AACGGCCCCC GAAGTCCCTG GGACGTCTCC
  781 CAGGGTTGCG GCCGGGTGTT CCGAACTCGT CAGTTCCACC ACGGGTCCGC CAGATACAGA
  841 GCTAGTTAGC TAACTAGTAC AGACGCACGC GCAAACATTA GATGCCGGCA CAAACACGCA
  901 CAAAGACAGA GACAGACACG AAACGACCGC TGGGCCAGCT TACCTCCCGG CGGGGGGGTC
  961 GGTGGTCCCT GGGCAGGGGT CTCCAATCTC GGTGGAACCT CCAAATGAAA GACCCCCGAG
 1021 GTGGGCAGTC AATCAATCTG AGGAGACCCT CCAGATCAGC GGAGTCACGA TCCGGATGCC
1081 AAACAGCCAG AGGCTTATGG ATACGTTACC GGCGACTCAG TTCAATCGGA GGTACTTGC
//
Primer: EGFP-C RDB12175_A6Ajf.seq
>12175_12175_A6Aj_EGFP-C_C08_08.ab1
    1 CCACGGGATC TCTCGGGGCA TGGGACGAGC TGTACAAGTA AAGCGGCCGG CCGCCAGCAC
   61 AGTGGTCGAC CGGGCCGCCA GCACAGTGGT CGATCGAGGG GGGGCCCGCG ATTAGTCCAA
  121 TTTGTTAAAG ACAGGATATC AGTGGTCCAG GCTCTAGTTT TGACTCAACA ATATCACCAG
  181 CTGAAGCCTA TAGAGTACGA GCCATAGATA GAATAAAAGA TTTTATTTAG TCTCCAGAAA
  241 AAGGGGGGAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT
  301 TGCAAGGCAT GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG
  361 AGAGACAGCA GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC
  421 AGGGCCAAGA ACAGTTGGAA CAGCAGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG
  481 TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGTCCCCAG ATGCGGTCCC GCCCTCAGCA
  541 GTTTCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG GACCTGAAAT GACCCTGTGC
  601 CTTATTTGAA CTAACCAATC AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG
  661 AGCTCAATAA AAGAGCCCAC AACCCCTCAC TCGGCGCGCC AGTCCTCCGA TAGACTGCGT
  721 CGCCCGGGTA CCCGTGTATC CAATAAACCC TCTTGCAGTT GCATCCGACT TGTGGTCTCG
  781 CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA TTGACTACCC GTCAGCGGGG GTCTTTCACA
  841 TGCAGCATGT ATCAAAATTA ATTTGGTTTT TTTTTCTTAA GTATTTACAT TAAATGGCCA
  901 TAGTTGCATT AAATGAATCG GCCAACGCGC GGGGAGAGGC GGGTTTGCGT ATTGGGCGCT
  961 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT
 1021 CAGCTCACTC AAGGCGGTAA TACGGTTATC CACAGGAATC AGGGGATAAC GGCAGAAGAA
1081 CATGTGAAGC TAAGGCCAGC AAGCCAGAAA TCGTAAAAAG GCGCGCGATT GGCTT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

2019.04.09

GNP_filter3_RDBDEP_html_190327.pl