RDB08061 - 8x3'Gli-BS-delta51-LucII

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8x3'Gli-BS-delta51-LucII

Reporter plasmid carring eight copies of Gli-binding site

Catalog number	RDB08061
Resource name	8x3'Gli-BS-delta51-LucII
Clone info.	Reporter plasmid carring eight copies of Gli-binding site.
Comment	Use 8xm3'Gli-BS-delta51-LucII (cat.# RDB08062) and delta51-LucII (cat.# RDB08068) as negative control.
Vector backbone	delta51-LucII (Plasmid)
Size of vector backbone	6.1 kb
Selectable markers	Amp^r
Gene/insert name	firefly (Photinus pyralis) luciferase cDNA
Depositor\|Developer	Sasaki, Hiroshi \|

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External Database
firefly (Photinus pyralis) luciferase

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Development, 124, 1313-1322 (1997)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Development, 124, 1313-1322 (1997)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Development, 124, 1313-1322 (1997).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB08061	8x3'Gli-BS-delta51-LucII	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original	Sasaki, H., A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development, 124 (7): 1313-1322 (1997). PMID 9118802.

Further references such as user reports and related articles (go to bottom)

Featured content	Dnaconda's recommendation EXR0001j (Japanese text)
Featured content	Dnaconda's recommendation EXR0074e (English text)
Featured content	Dnaconda's recommendation EXR0074j (Japanese text)
Featured content	Promoter Collection (Outline) (English text)
Featured content	Promoter Collection (Outline) (Japanese text)
Featured content	Hedgehog signaling (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB08061_A6Ddp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL3_RV6R Region: chicken delta-crystallin Basal Pro,8x3'Gli-BS,SV40 pA Sequence file: RDB08061_A6Ddd.seq
>08061_08061_A6Dd_pGL3-RV6R_D01_10.ab1 1 NNNNNNNNNN NNNNNNNNNG NCGCCNGGCC TTTCTTTATG TTTTTGGCGT CTTCCNTTTT 61 ACCAACAGTA CCGGAATGCC AAGCTTGGCT GCCCGCACAG CCCTGCTCAC CGTCTCCGCA 121 GCTCAGCCCT GGCTGGTCGC TCCGTGCTCT CCGGTCTCAC AGCCCCGCTT TTACGTGCCC 181 CGGAGCCTGG GCTGGCCCTG CAGGTCGACT CTAGAGGATC CCCGGGAACA GATTCGCGAT 241 CGAGAGCTTC TACTTGGGTG TTCCCTGCTT GTCGGAGCTT CTACTTGGGT GTTCCCTGCT 301 TGTCGAGGTC GAGAGCTTCT ACTTGGGTGT TCCCTGCTTG TCGGAGCTTC TACTTGGGTG 361 TTCCCTGCTT GTCGAGGTCG AGAGCTTCTA CTTGGGTGTT CCCTGCTTGT CGGAGCTTCT 421 ACTTGGGTGT TCCCTGCTTG TCGAGGTCGA GAGCTTCTAC TTGGGTGTTC CCTGCTTGTC 481 GGAACTTCTA CTTGGGTGTT CCCTGCTTGT CGAGGTCGAT CGCGAATCTG TTCCCGGGGA 541 TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA 601 AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG 661 CAATAAACAA GTTAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGANGTGTG 721 GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CNAATGTGAT ATGGCTGATC CAGACATGAT 781 AAGATACNTT GATGAGTTTG GACNANCCAC AACTANAANT GCAGTGAAAA AAATGCTTTA 841 TTTGTGNAAT TTGTGATGCT ATTGCTTNAT TTGTAACNNT NNNNNNNNTG CNNTAAACNA 901 GTTANNNNNN ANNTGCNNTC ATTTTTATGT TTNNNNGTTC NNGGGGNNNN NGNNGGNNNG 961 NNNTTTTTNN NNNNNNNN //
Primer: RDB08068_F1 Region: SV40 pA Sequence file: RDB08061_A6Dde.seq
>08061_08061_A6Dd_RDB8068_F1_C12_03_ABI08.ab1 1 NNNNNNNNNN NNNNNGNNNG GNNNTCCNNG ANANTTTAAA ATTACCCTTA GAAAGCGGTC 61 TGTGAAAAAC CCCTACCCAA TTTCCTTTTT GTTAAGTGAC CTAATTAACA GGAGGACACA 121 GAGGGTGGAT GGGCAGCCTA TGATTGGAAT GTCCTCTCAA GTAGAGGAGG TTAGGGTTTA 181 TGAGGACACA GAGGAGCTTC CTGGGCCTCG AGGCAGACAT GATAAGATAC ATTGATGAGT 241 TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG 301 CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA 361 TTCATTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC 421 TCTNNNNNNG TGGTATGGCT GATTATGATC TCTNNNNNNN GNNCTNTNNN TNNNATATTC 481 CTTATNANNN NCNNNNNNNA NTAAAAAGCT AAANGNNNNC AATTTNNNNN NATANNNATT 541 AANNNNANAC NCTNNNNNCC TGTGTGGAGT AANANNNNAN NNNNNGNNNN NNNNNN //
Primer: Luc-C_F Region: Luc 3',small t intron,SV40 NLS Sequence file: RDB08061_A6Ddf.seq
>08061_08061_A6Dd_Luc-C_F_B12_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNCNG NNNNNAACNA CCGCGNNNNN NTGCGCGGAG GAGTTGTGTT 61 TGTGGACGAA GTACCGAAAG GTCTTACCGG AAAACTCGAC GCAAGAAAAA TCAGAGAGAT 121 CCTCATAAAG GCCAAGAAGG GCGGAAAGTC CAAATTGTAA AATGTAACTG TATTCAGCGA 181 TGACGAAATT CTTAGCTATT GTAATACTCT AGAGGATCTT TGTGAAGGAA CCTTACTTCT 241 GTGGTGTGAC ATAATTGGAC AAACTACCTA CAGAGATTTA AAGCTCTAAG GTAAATATAA 301 AATTTTTAAG TGTATAATGT GTTAAACTAC TGATTCTAAT TGTTTGTGTA TTTTAGATTC 361 CAACCTATGG AACTGATGAA TGGGAGCAGT GGTGGAATGC CTTTAATGAG GAAAACCTGT 421 TTTGCTCAGA AGAAATGCCA TCTAGTGATG ATGAGGCTAC TGCTGACTCT CAACATTCTA 481 CTCCTCCAAA AAAGAAGAGA AAGGTAGAAG ACCCCAAGGA CTTTCCTTCA GAATTGCTAA 541 GTTTTTTGAG TCATGCTGTG TTTAGTAATA GAACTCTTGC TTGCTTTGCT ATTTACACCA 601 CAAAGGAAAA AGCTGCACTG CTATACAAGA AAATTATGGA AAAATATTCT GTAACCTTTA 661 TAAGTAGGCA TAACAGTTAT AATCATAACA TACTGTTTTT TCTTACTCCA CACAGGCATA 721 GAGTGTCTGC TATTAATAAC TATGCTCAAA AATTGTGTAC CTTTAGCTTT TTAANTTGTA 781 AAGGGGTTAA TAAGGAATAT TTGATGTATA GTGNCTTGAC TAGAGATCAT ANTCAGNNNN 841 ANCACNNTTG TAGANGGNTT NACTTGCNTN NAAAANNCTC CCACNNCCTC CCCCTGANNC 901 TGAANCNTNA ANNGAATGCA ATTGNTNGNN GTNNANNTNG TTTANTGCNN NTNNNNNNGG 961 TTACNANNNN NGCANNNNNN NCNCNANNTT NANNANNANN CNTTTTTTTT CNNTGCNNTN 1021 NTNNTNGNGG NTNNNCNANC TCNNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Sasaki, H., A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development, 124 (7): 1313-1322 (1997). PMID 9118802.
reference	Gunarta, I.K., Critical role of glioma-associated oncogene homolog 1 in maintaining invasive and mesenchymal-like properties of melanoma cells. Cancer Sci. 108 (8): 1602-1611 (2017). PMID 28635133.
user_report	Li, L., Upregulation of amplified in breast cancer 1 contributes to pancreatic ductal adenocarcinoma progression and vulnerability to blockage of hedgehog activation. Theranostics 11 (4): 1672-1689 (2021). PMID 33408774.
user_report	Dettmer, R., FGF2 Inhibits Early Pancreatic Lineage Specification during Differentiation of Human Embryonic Stem Cells. Cells 9 (9): 1927 (2020). PMID 32825270.
user_report	Babu, D., Novel GLI2 mutations identified in patients with Combined Pituitary Hormone Deficiency (CPHD): Evidence for a pathogenic effect by functional characterization. Clin. Endocrinol. (Oxf). 90 (3): 449-456 (2019). PMID 30548673.
user_report	Torrado, B., Ciliary Entry of the Hedgehog Transcriptional Activator Gli2 Is Mediated by the Nuclear Import Machinery but Differs from Nuclear Transport in Being Imp-alpha/beta1-Independent. PLoS One 11 (8): e0162033 (2016). PMID 27579771.
user_report	Sugiyama, M., Paired related homeobox 1 is associated with the invasive properties of glioblastoma cells. Oncol. Rep. 33 (3): 1123-1130 (2015). PMID 25522823.
user_report	Shiohama, T., Oxidative stress-induced JNK1 phosphorylation inhibits hedgehog signalling and osteoblast differentiation. Cell Biol. Int. Rep. 21 (2); 53-62 (2014).
user_report	Wang, D.H., Hedgehog signaling regulates FOXA2 in esophageal embryogenesis and Barrett's metaplasia. J. Clin. Invest. 124 (9): 3767-80 (2014). PMID 25083987.

2022.05.17

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