Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

8x3'Gli-BS-delta51-LucII (#RDB08061)

Reporter plasmid carring eight copies of Gli-binding site

Clone info. Reporter plasmid carring eight copies of Gli-binding site.
Comment Use 8xm3'Gli-BS-delta51-LucII (cat.# RDB08062) and delta51-LucII (cat.# RDB08068) as negative control.
Vector backbone delta51-LucII (RDB08068) (Plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Development, 124, 1313-1322 (1997)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Development, 124, 1313-1322 (1997).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08061 8x3'Gli-BS-delta51-LucII DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The 8x3'Gli-BS-delta51-LucII was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08061).

Reference section:

Sasaki, H., Hui, C., Nakafuku, M., Kondoh, H., A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development, 124 (7): 1313-1322 (1997). PMID 9118802. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08061_A6Ddp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL3_RV6R (Pr0228)
Region: chicken delta-crystallin Basal Pro,8x3'Gli-BS,SV40 pA
Sequence file: RDB08061_A6Ddd.seq check
>08061_08061_A6Dd_pGL3-RV6R_D01_10.ab1
    1 NNNNNNNNNN NNNNNNNNNG NCGCCNGGCC TTTCTTTATG TTTTTGGCGT CTTCCNTTTT
   61 ACCAACAGTA CCGGAATGCC AAGCTTGGCT GCCCGCACAG CCCTGCTCAC CGTCTCCGCA
  121 GCTCAGCCCT GGCTGGTCGC TCCGTGCTCT CCGGTCTCAC AGCCCCGCTT TTACGTGCCC
  181 CGGAGCCTGG GCTGGCCCTG CAGGTCGACT CTAGAGGATC CCCGGGAACA GATTCGCGAT
  241 CGAGAGCTTC TACTTGGGTG TTCCCTGCTT GTCGGAGCTT CTACTTGGGT GTTCCCTGCT
  301 TGTCGAGGTC GAGAGCTTCT ACTTGGGTGT TCCCTGCTTG TCGGAGCTTC TACTTGGGTG
  361 TTCCCTGCTT GTCGAGGTCG AGAGCTTCTA CTTGGGTGTT CCCTGCTTGT CGGAGCTTCT
  421 ACTTGGGTGT TCCCTGCTTG TCGAGGTCGA GAGCTTCTAC TTGGGTGTTC CCTGCTTGTC
  481 GGAACTTCTA CTTGGGTGTT CCCTGCTTGT CGAGGTCGAT CGCGAATCTG TTCCCGGGGA
  541 TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA
  601 AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG
  661 CAATAAACAA GTTAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGANGTGTG
  721 GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CNAATGTGAT ATGGCTGATC CAGACATGAT
  781 AAGATACNTT GATGAGTTTG GACNANCCAC AACTANAANT GCAGTGAAAA AAATGCTTTA
  841 TTTGTGNAAT TTGTGATGCT ATTGCTTNAT TTGTAACNNT NNNNNNNNTG CNNTAAACNA
  901 GTTANNNNNN ANNTGCNNTC ATTTTTATGT TTNNNNGTTC NNGGGGNNNN NGNNGGNNNG
961 NNNTTTTTNN NNNNNNNN
//
Primer: RDB08068_F1 (Pr0221)
Region: SV40 pA
Sequence file: RDB08061_A6Dde.seq check
>08061_08061_A6Dd_RDB8068_F1_C12_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNGNNNG GNNNTCCNNG ANANTTTAAA ATTACCCTTA GAAAGCGGTC
   61 TGTGAAAAAC CCCTACCCAA TTTCCTTTTT GTTAAGTGAC CTAATTAACA GGAGGACACA
  121 GAGGGTGGAT GGGCAGCCTA TGATTGGAAT GTCCTCTCAA GTAGAGGAGG TTAGGGTTTA
  181 TGAGGACACA GAGGAGCTTC CTGGGCCTCG AGGCAGACAT GATAAGATAC ATTGATGAGT
  241 TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG
  301 CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA
  361 TTCATTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC
  421 TCTNNNNNNG TGGTATGGCT GATTATGATC TCTNNNNNNN GNNCTNTNNN TNNNATATTC
  481 CTTATNANNN NCNNNNNNNA NTAAAAAGCT AAANGNNNNC AATTTNNNNN NATANNNATT
541 AANNNNANAC NCTNNNNNCC TGTGTGGAGT AANANNNNAN NNNNNGNNNN NNNNNN
//
Primer: Luc-C_F (Pr0331)
Region: Luc 3',small t intron,SV40 NLS
Sequence file: RDB08061_A6Ddf.seq check
>08061_08061_A6Dd_Luc-C_F_B12_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNCNG NNNNNAACNA CCGCGNNNNN NTGCGCGGAG GAGTTGTGTT
   61 TGTGGACGAA GTACCGAAAG GTCTTACCGG AAAACTCGAC GCAAGAAAAA TCAGAGAGAT
  121 CCTCATAAAG GCCAAGAAGG GCGGAAAGTC CAAATTGTAA AATGTAACTG TATTCAGCGA
  181 TGACGAAATT CTTAGCTATT GTAATACTCT AGAGGATCTT TGTGAAGGAA CCTTACTTCT
  241 GTGGTGTGAC ATAATTGGAC AAACTACCTA CAGAGATTTA AAGCTCTAAG GTAAATATAA
  301 AATTTTTAAG TGTATAATGT GTTAAACTAC TGATTCTAAT TGTTTGTGTA TTTTAGATTC
  361 CAACCTATGG AACTGATGAA TGGGAGCAGT GGTGGAATGC CTTTAATGAG GAAAACCTGT
  421 TTTGCTCAGA AGAAATGCCA TCTAGTGATG ATGAGGCTAC TGCTGACTCT CAACATTCTA
  481 CTCCTCCAAA AAAGAAGAGA AAGGTAGAAG ACCCCAAGGA CTTTCCTTCA GAATTGCTAA
  541 GTTTTTTGAG TCATGCTGTG TTTAGTAATA GAACTCTTGC TTGCTTTGCT ATTTACACCA
  601 CAAAGGAAAA AGCTGCACTG CTATACAAGA AAATTATGGA AAAATATTCT GTAACCTTTA
  661 TAAGTAGGCA TAACAGTTAT AATCATAACA TACTGTTTTT TCTTACTCCA CACAGGCATA
  721 GAGTGTCTGC TATTAATAAC TATGCTCAAA AATTGTGTAC CTTTAGCTTT TTAANTTGTA
  781 AAGGGGTTAA TAAGGAATAT TTGATGTATA GTGNCTTGAC TAGAGATCAT ANTCAGNNNN
  841 ANCACNNTTG TAGANGGNTT NACTTGCNTN NAAAANNCTC CCACNNCCTC CCCCTGANNC
  901 TGAANCNTNA ANNGAATGCA ATTGNTNGNN GTNNANNTNG TTTANTGCNN NTNNNNNNGG
  961 TTACNANNNN NGCANNNNNN NCNCNANNTT NANNANNANN CNTTTTTTTT CNNTGCNNTN
1021 NTNNTNGNGG NTNNNCNANC TCNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Sasaki, H., A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development, 124 (7): 1313-1322 (1997). PMID 9118802. [link to RRC of NBRP]
reference Gunarta, I.K., Critical role of glioma-associated oncogene homolog 1 in maintaining invasive and mesenchymal-like properties of melanoma cells. Cancer Sci. 108 (8): 1602-1611 (2017). PMID 28635133.
user_report Li, L., Upregulation of amplified in breast cancer 1 contributes to pancreatic ductal adenocarcinoma progression and vulnerability to blockage of hedgehog activation. Theranostics 11 (4): 1672-1689 (2021). PMID 33408774. [link to RRC of NBRP]
user_report Dettmer, R., FGF2 Inhibits Early Pancreatic Lineage Specification during Differentiation of Human Embryonic Stem Cells. Cells 9 (9): 1927 (2020). PMID 32825270. [link to RRC of NBRP]
user_report Babu, D., Novel GLI2 mutations identified in patients with Combined Pituitary Hormone Deficiency (CPHD): Evidence for a pathogenic effect by functional characterization. Clin. Endocrinol. (Oxf). 90 (3): 449-456 (2019). PMID 30548673. [link to RRC of NBRP]
user_report Torrado, B., Ciliary Entry of the Hedgehog Transcriptional Activator Gli2 Is Mediated by the Nuclear Import Machinery but Differs from Nuclear Transport in Being Imp-alpha/beta1-Independent. PLoS One 11 (8): e0162033 (2016). PMID 27579771. [link to RRC of NBRP]
user_report Sugiyama, M., Paired related homeobox 1 is associated with the invasive properties of glioblastoma cells. Oncol. Rep. 33 (3): 1123-1130 (2015). PMID 25522823. [link to RRC of NBRP]
user_report Shiohama, T., Oxidative stress-induced JNK1 phosphorylation inhibits hedgehog signalling and osteoblast differentiation. Cell Biol. Int. Rep. 21 (2); 53-62 (2014). [link to RRC of NBRP]
user_report Wang, D.H., Hedgehog signaling regulates FOXA2 in esophageal embryogenesis and Barrett's metaplasia. J. Clin. Invest. 124 (9): 3767-80 (2014). PMID 25083987. [link to RRC of NBRP]