RDB08061 - 8x3'Gli-BS-delta51-LucII

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8x3'Gli-BS-delta51-LucII

Reporter plasmid carring eight copies of Gli-binding site

Catalog number	RDB08061
Resource name	8x3'Gli-BS-delta51-LucII
Clone info.	Reporter plasmid carring eight copies of Gli-binding site. Use 8xm3'Gli-BS-delta51-LucII (cat.# RDB08062) and delta51-LucII (cat.# RDB08068) as negative control.
Vector backbone	delta51-LucII (Plasmid)
Size of vector backbone	6.1 kb
Selectable markers	Amp^r
Gene/insert name	firefly (Photinus pyralis) luciferase cDNA
Depositor\|Developer	Sasaki, Hiroshi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Development, 124, 1313-1322 (1997)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Development, 124, 1313-1322 (1997)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Development, 124, 1313-1322 (1997).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB08061	8x3'Gli-BS-delta51-LucII	DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original	Sasaki, H., A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development, 124 (7): 1313-1322 (1997). PMID 9118802.

Further references such as user reports and related articles (go to bottom)

Featured content	Dnaconda's recommendation EXR0001j (Japanese text)
Featured content	Dnaconda's recommendation EXR0074j (Japanese text)
Featured content	Dnaconda's recommendation EXR0074e (English text)
Featured content	Promoter Collection (Outline) (English text)
Featured content	Promoter Collection (Outline) (Japanese text)
Featured content	Hedgehog signaling (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB08061_A6Ddp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL3-RV6R Sequence file: RDB08061_A6Dda.seq
>08061_08061_A6Dd_pGL3-RV6R_D01_10.ab1 1 GTTCTCCTCA AGAGAAGATG GCGCCGGGCC TTTCTTTATG TTTTTGGCGT CTTCCATTTT 61 ACCAACAGTA CCGGAATGCC AAGCTTGGCT GCCCGCACAG CCCTGCTCAC CGTCTCCGCA 121 GCTCAGCCCT GGCTGGTCGC TCCGTGCTCT CCGGTCTCAC AGCCCCGCTT TTACGTGCCC 181 CGGAGCCTGG GCTGGCCCTG CAGGTCGACT CTAGAGGATC CCCGGGAACA GATTCGCGAT 241 CGAGAGCTTC TACTTGGGTG TTCCCTGCTT GTCGGAGCTT CTACTTGGGT GTTCCCTGCT 301 TGTCGAGGTC GAGAGCTTCT ACTTGGGTGT TCCCTGCTTG TCGGAGCTTC TACTTGGGTG 361 TTCCCTGCTT GTCGAGGTCG AGAGCTTCTA CTTGGGTGTT CCCTGCTTGT CGGAGCTTCT 421 ACTTGGGTGT TCCCTGCTTG TCGAGGTCGA GAGCTTCTAC TTGGGTGTTC CCTGCTTGTC 481 GGAACTTCTA CTTGGGTGTT CCCTGCTTGT CGAGGTCGAT CGCGAATCTG TTCCCGGGGA 541 TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA 601 AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG 661 CAATAAACAA GTTAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG 721 GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGAT ATGGCTGATC CAGACATGAT 781 AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAAAT GCAGTGAAAA AAATGCTTTA 841 TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAAGCTG CAATAAACAA 901 GTTAACAACA AATTGCATTC ATTTTTATGT TTTCAGGTTC AGGGGGGAGG TGTGGGGAGG 961 GTTTTTTTAA AGCTAGTTAA AACCTTCTAC CAATGTGATA TTGGGCTTGA ATTCCTGCCT 1021 CGCACGTTTT TCGGTTGAAT GAACGGATGA AAAAACACAT CCTGACAACA TGGCAAACTT 1081 CCCCTGAGAG AACGGGTCTA CACACTTTGA TCCAGAAAGT CGAGGATGTC CGAGGAACTC 1141 GACATGACCA CCGATCAAGG GACGGCCTTA CGACAGTGAT GCTGTCTATT GTGCATCGTG 1201 AGCACGTGAC TC //
Primer: Luc-C_F Sequence file: RDB08061_A6Ddb.seq
>08061_08061_A6Dd_Luc-C_F_B12_02_ABI08.ab1 1 GAAAAATACG GGTCGGCCAG TCAAGTAACA ACCGCGAAAA AGTTGCGCGG AGGAGTTGTG 61 TTTGTGGACG AAGTACCGAA AGGTCTTACC GGAAAACTCG ACGCAAGAAA AATCAGAGAG 121 ATCCTCATAA AGGCCAAGAA GGGCGGAAAG TCCAAATTGT AAAATGTAAC TGTATTCAGC 181 GATGACGAAA TTCTTAGCTA TTGTAATACT CTAGAGGATC TTTGTGAAGG AACCTTACTT 241 CTGTGGTGTG ACATAATTGG ACAAACTACC TACAGAGATT TAAAGCTCTA AGGTAAATAT 301 AAAATTTTTA AGTGTATAAT GTGTTAAACT ACTGATTCTA ATTGTTTGTG TATTTTAGAT 361 TCCAACCTAT GGAACTGATG AATGGGAGCA GTGGTGGAAT GCCTTTAATG AGGAAAACCT 421 GTTTTGCTCA GAAGAAATGC CATCTAGTGA TGATGAGGCT ACTGCTGACT CTCAACATTC 481 TACTCCTCCA AAAAAGAAGA GAAAGGTAGA AGACCCCAAG GACTTTCCTT CAGAATTGCT 541 AAGTTTTTTG AGTCATGCTG TGTTTAGTAA TAGAACTCTT GCTTGCTTTG CTATTTACAC 601 CACAAAGGAA AAAGCTGCAC TGCTATACAA GAAAATTATG GAAAAATATT CTGTAACCTT 661 TATAAGTAGG CATAACAGTT ATAATCATAA CATACTGTTT TTTCTTACTC CACACAGGCA 721 TAGAGTGTCT GCTATTAATA ACTATGCTCA AAAATTGTGT ACCTTTAGCT TTTTAATTTG 781 TAAAGGGGTT AATAAGGAAT ATTTGATGTA TAGTGCCTTG ACTAGAGATC ATAATCAGCC 841 ATACCACATT TGTAGAGGGT TTTACTTGCT TTAAAAAACC TCCCACACCC TCCCCCCTGA 901 ACCTGAAACA TAAAATGAAT GCAATTGTTT GTTTGTTAAC TTTGTTTATT GCAGCTTATA 961 TGGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAT AAAGCATTTT TTTCACTGCA 1021 TTTCTAGTTG TGGTTTGTCA AACTCATTCA TGTATCTTTA TCATGTCCTG GCTCGAAGCC 1081 CAGAAAGCTC CTCTGTGTCC CTCATAAACC CTAACCTTCC TCTACCTTGA GAGGAACATT 1141 CCAATCATAG GGCTGTCCAT TCCACCTTCT TGGTCCTCCC TGTTTAATAT AGGGTTCACT 1201 TTAACCAAAA GGAAAATGTG GAGAGGGCGT CTTTC //
Primer: RDB08068_F1 Sequence file: RDB08061_A6Ddc.seq
>08061_08061_A6Dd_RDB8068_F1_C12_03_ABI08.ab1 1 CCAGGACCAG ATTGGAAGGG GACTTCCCAG ATATTTTAAA ATTACCCTTA GAAAGCGGTC 61 TGTGAAAAAC CCCTACCCAA TTTCCTTTTT GTTAAGTGAC CTAATTAACA GGAGGACACA 121 GAGGGTGGAT GGGCAGCCTA TGATTGGAAT GTCCTCTCAA GTAGAGGAGG TTAGGGTTTA 181 TGAGGACACA GAGGAGCTTC CTGGGCCTCG AGGCAGACAT GATAAGATAC ATTGATGAGT 241 TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG 301 CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA 361 TTCATTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Sasaki, H., A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development, 124 (7): 1313-1322 (1997). PMID 9118802.
reference	Gunarta, I.K., Critical role of glioma-associated oncogene homolog 1 in maintaining invasive and mesenchymal-like properties of melanoma cells. Cancer Sci. 108 (8): 1602-1611 (2017). PMID 28635133.
user report	Dettmer, R., FGF2 Inhibits Early Pancreatic Lineage Specification during Differentiation of Human Embryonic Stem Cells. Cells 9 (9): 1927 (2020). PMID 32825270.
user report	Babu, D., Novel GLI2 mutations identified in patients with Combined Pituitary Hormone Deficiency (CPHD): Evidence for a pathogenic effect by functional characterization. Clin. Endocrinol. (Oxf). 90 (3): 449-456 (2019). PMID 30548673.
user report	Torrado, B., Ciliary Entry of the Hedgehog Transcriptional Activator Gli2 Is Mediated by the Nuclear Import Machinery but Differs from Nuclear Transport in Being Imp-alpha/beta1-Independent. PLoS One 11 (8): e0162033 (2016). PMID 27579771.
user report	Sugiyama, M., Paired related homeobox 1 is associated with the invasive properties of glioblastoma cells. Oncol. Rep. 33 (3): 1123-1130 (2015). PMID 25522823.
user report	Shiohama, T., Oxidative stress-induced JNK1 phosphorylation inhibits hedgehog signalling and osteoblast differentiation. Cell Biol. Int. Rep. 21 (2); 53-62 (2014).
user report	Wang, D.H., Hedgehog signaling regulates FOXA2 in esophageal embryogenesis and Barrett's metaplasia. J. Clin. Invest. 124 (9): 3767-80 (2014). PMID 25083987.

2020.12.30

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