Sequencing and PCR primers for primer information.
Reporter plasmid carring eight copies of Gli-binding site
| Catalog number | RDB08061 |
|---|---|
| Resource name | 8x3'Gli-BS-delta51-LucII |
| Clone info. | Reporter plasmid carring eight copies of Gli-binding site. Use 8xm3'Gli-BS-delta51-LucII (cat.# RDB08062) and delta51-LucII (cat.# RDB08068) as negative control. |
| Vector backbone | delta51-LucII (Plasmid) |
| Size of vector backbone | 6.1 kb |
| Selectable markers | Amp^r |
| Gene/insert name | firefly (Photinus pyralis) luciferase cDNA |
| Depositor | Sasaki, Hiroshi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Development, 124, 1313-1322 (1997).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB08061 | 8x3'Gli-BS-delta51-LucII | DNA solution |
Electronic file
Featured content
| Featured content | Dnaconda's recommendation EXR0001j (Japanese text) |
|---|---|
| Featured content | Promoter Collection (Outline) (English text) |
| Featured content | Promoter Collection (Outline) (Japanese text) |
| Featured content | Hedgehog signaling (English text) |
References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB08061_A6Ddp1-1.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
| Primer: pGL3-RV6R | RDB08061_A6Dda.seq |
|---|---|
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>08061_08061_A6Dd_pGL3-RV6R_D01_10.ab1 1 GTTCTCCTCA AGAGAAGATG GCGCCGGGCC TTTCTTTATG TTTTTGGCGT CTTCCATTTT 61 ACCAACAGTA CCGGAATGCC AAGCTTGGCT GCCCGCACAG CCCTGCTCAC CGTCTCCGCA 121 GCTCAGCCCT GGCTGGTCGC TCCGTGCTCT CCGGTCTCAC AGCCCCGCTT TTACGTGCCC 181 CGGAGCCTGG GCTGGCCCTG CAGGTCGACT CTAGAGGATC CCCGGGAACA GATTCGCGAT 241 CGAGAGCTTC TACTTGGGTG TTCCCTGCTT GTCGGAGCTT CTACTTGGGT GTTCCCTGCT 301 TGTCGAGGTC GAGAGCTTCT ACTTGGGTGT TCCCTGCTTG TCGGAGCTTC TACTTGGGTG 361 TTCCCTGCTT GTCGAGGTCG AGAGCTTCTA CTTGGGTGTT CCCTGCTTGT CGGAGCTTCT 421 ACTTGGGTGT TCCCTGCTTG TCGAGGTCGA GAGCTTCTAC TTGGGTGTTC CCTGCTTGTC 481 GGAACTTCTA CTTGGGTGTT CCCTGCTTGT CGAGGTCGAT CGCGAATCTG TTCCCGGGGA 541 TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA 601 AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG 661 CAATAAACAA GTTAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG 721 GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGAT ATGGCTGATC CAGACATGAT 781 AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAAAT GCAGTGAAAA AAATGCTTTA 841 TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAAGCTG CAATAAACAA 901 GTTAACAACA AATTGCATTC ATTTTTATGT TTTCAGGTTC AGGGGGGAGG TGTGGGGAGG 961 GTTTTTTTAA AGCTAGTTAA AACCTTCTAC CAATGTGATA TTGGGCTTGA ATTCCTGCCT 1021 CGCACGTTTT TCGGTTGAAT GAACGGATGA AAAAACACAT CCTGACAACA TGGCAAACTT 1081 CCCCTGAGAG AACGGGTCTA CACACTTTGA TCCAGAAAGT CGAGGATGTC CGAGGAACTC 1141 GACATGACCA CCGATCAAGG GACGGCCTTA CGACAGTGAT GCTGTCTATT GTGCATCGTG 1201 AGCACGTGAC TC // |
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| Primer: Luc-C_F | RDB08061_A6Ddb.seq |
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>08061_08061_A6Dd_Luc-C_F_B12_02_ABI08.ab1 1 GAAAAATACG GGTCGGCCAG TCAAGTAACA ACCGCGAAAA AGTTGCGCGG AGGAGTTGTG 61 TTTGTGGACG AAGTACCGAA AGGTCTTACC GGAAAACTCG ACGCAAGAAA AATCAGAGAG 121 ATCCTCATAA AGGCCAAGAA GGGCGGAAAG TCCAAATTGT AAAATGTAAC TGTATTCAGC 181 GATGACGAAA TTCTTAGCTA TTGTAATACT CTAGAGGATC TTTGTGAAGG AACCTTACTT 241 CTGTGGTGTG ACATAATTGG ACAAACTACC TACAGAGATT TAAAGCTCTA AGGTAAATAT 301 AAAATTTTTA AGTGTATAAT GTGTTAAACT ACTGATTCTA ATTGTTTGTG TATTTTAGAT 361 TCCAACCTAT GGAACTGATG AATGGGAGCA GTGGTGGAAT GCCTTTAATG AGGAAAACCT 421 GTTTTGCTCA GAAGAAATGC CATCTAGTGA TGATGAGGCT ACTGCTGACT CTCAACATTC 481 TACTCCTCCA AAAAAGAAGA GAAAGGTAGA AGACCCCAAG GACTTTCCTT CAGAATTGCT 541 AAGTTTTTTG AGTCATGCTG TGTTTAGTAA TAGAACTCTT GCTTGCTTTG CTATTTACAC 601 CACAAAGGAA AAAGCTGCAC TGCTATACAA GAAAATTATG GAAAAATATT CTGTAACCTT 661 TATAAGTAGG CATAACAGTT ATAATCATAA CATACTGTTT TTTCTTACTC CACACAGGCA 721 TAGAGTGTCT GCTATTAATA ACTATGCTCA AAAATTGTGT ACCTTTAGCT TTTTAATTTG 781 TAAAGGGGTT AATAAGGAAT ATTTGATGTA TAGTGCCTTG ACTAGAGATC ATAATCAGCC 841 ATACCACATT TGTAGAGGGT TTTACTTGCT TTAAAAAACC TCCCACACCC TCCCCCCTGA 901 ACCTGAAACA TAAAATGAAT GCAATTGTTT GTTTGTTAAC TTTGTTTATT GCAGCTTATA 961 TGGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAT AAAGCATTTT TTTCACTGCA 1021 TTTCTAGTTG TGGTTTGTCA AACTCATTCA TGTATCTTTA TCATGTCCTG GCTCGAAGCC 1081 CAGAAAGCTC CTCTGTGTCC CTCATAAACC CTAACCTTCC TCTACCTTGA GAGGAACATT 1141 CCAATCATAG GGCTGTCCAT TCCACCTTCT TGGTCCTCCC TGTTTAATAT AGGGTTCACT 1201 TTAACCAAAA GGAAAATGTG GAGAGGGCGT CTTTC // |
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| Primer: RDB08068_F1 | RDB08061_A6Ddc.seq |
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>08061_08061_A6Dd_RDB8068_F1_C12_03_ABI08.ab1 1 CCAGGACCAG ATTGGAAGGG GACTTCCCAG ATATTTTAAA ATTACCCTTA GAAAGCGGTC 61 TGTGAAAAAC CCCTACCCAA TTTCCTTTTT GTTAAGTGAC CTAATTAACA GGAGGACACA 121 GAGGGTGGAT GGGCAGCCTA TGATTGGAAT GTCCTCTCAA GTAGAGGAGG TTAGGGTTTA 181 TGAGGACACA GAGGAGCTTC CTGGGCCTCG AGGCAGACAT GATAAGATAC ATTGATGAGT 241 TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG 301 CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA 361 TTCATTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTT // |
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Please visit Sequencing and PCR primers for primer information.
References
2019.03.07
GNP_filter3_RDBDEP_html_190307.pl