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8x3'Gli-BS-delta51-LucII (#RDB08061)

Reporter plasmid carring eight copies of Gli-binding site


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Sasaki, H., Development, 124 (7): 1313-1322 (1997). PMID 9118802. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Reporter plasmid carring eight copies of Gli-binding site.
Comment Use 8xm3'Gli-BS-delta51-LucII (RDB08062) and delta51-LucII (RDB08068) as negative control.
Vector backbone delta51-LucII (RDB08068) (Plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r
Growth conditions 37℃, LB+Amp
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor|Developer Sasaki, Hiroshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Development, 124, 1313-1322 (1997)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Development, 124, 1313-1322 (1997).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee
RDB08061 8x3'Gli-BS-delta51-LucII DNA solution Please contact us.

How to cite this biological resource

Materials & Methods section:

The 8x3'Gli-BS-delta51-LucII was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08061).

Reference section:

Sasaki, H., Hui, C., Nakafuku, M., Kondoh, H., A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development, 124 (7): 1313-1322 (1997). PMID 9118802. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Sasaki, H., A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development, 124 (7): 1313-1322 (1997). PMID 9118802. [PubMed] [Article] [RRC of NBRP]
reference Gunarta, I.K., Critical role of glioma-associated oncogene homolog 1 in maintaining invasive and mesenchymal-like properties of melanoma cells. Cancer Sci. 108 (8): 1602-1611 (2017). PMID 28635133. [PubMed] [Article]
user_report Leung, A.O.W., Suppression of apoptosis impairs phalangeal joint formation in the pathogenesis of brachydactyly type A1. Nat. Commun. 15 (1): 2229 (2024). PMID 38472182. [PubMed] [Article] [RRC of NBRP]
user_report Li, L., Upregulation of amplified in breast cancer 1 contributes to pancreatic ductal adenocarcinoma progression and vulnerability to blockage of hedgehog activation. Theranostics 11 (4): 1672-1689 (2021). PMID 33408774. [PubMed] [Article] [RRC of NBRP]
user_report Dettmer, R., FGF2 Inhibits Early Pancreatic Lineage Specification during Differentiation of Human Embryonic Stem Cells. Cells 9 (9): 1927 (2020). PMID 32825270. [PubMed] [Article] [RRC of NBRP]
user_report Babu, D., Novel GLI2 mutations identified in patients with Combined Pituitary Hormone Deficiency (CPHD): Evidence for a pathogenic effect by functional characterization. Clin. Endocrinol. (Oxf). 90 (3): 449-456 (2019). PMID 30548673. [PubMed] [Article] [RRC of NBRP]
user_report Torrado, B., Ciliary Entry of the Hedgehog Transcriptional Activator Gli2 Is Mediated by the Nuclear Import Machinery but Differs from Nuclear Transport in Being Imp-alpha/beta1-Independent. PLoS One 11 (8): e0162033 (2016). PMID 27579771. [PubMed] [Article] [RRC of NBRP]
user_report Sugiyama, M., Paired related homeobox 1 is associated with the invasive properties of glioblastoma cells. Oncol. Rep. 33 (3): 1123-1130 (2015). PMID 25522823. [PubMed] [Article] [RRC of NBRP]
user_report Shiohama, T., Oxidative stress-induced JNK1 phosphorylation inhibits hedgehog signalling and osteoblast differentiation. Cell Biol. Int. Rep. 21 (2); 53-62 (2014). [Article] [RRC of NBRP]
user_report Wang, D.H., Hedgehog signaling regulates FOXA2 in esophageal embryogenesis and Barrett's metaplasia. J. Clin. Invest. 124 (9): 3767-80 (2014). PMID 25083987. [PubMed] [Article] [RRC of NBRP]