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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

8x3'Gli-BS-delta51-LucII

Reporter plasmid carring eight copies of Gli-binding site

Catalog number RDB08061
Resource name 8x3'Gli-BS-delta51-LucII
Clone info. Reporter plasmid carring eight copies of Gli-binding site.
Comment Use 8xm3'Gli-BS-delta51-LucII (cat.# RDB08062) and delta51-LucII (cat.# RDB08068) as negative control.
Vector backbone delta51-LucII (Plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor|Developer Sasaki, Hiroshi |
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External Database
firefly (Photinus pyralis) luciferase

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Development, 124, 1313-1322 (1997)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Development, 124, 1313-1322 (1997).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB08061 8x3'Gli-BS-delta51-LucII DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Sasaki, H., A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development, 124 (7): 1313-1322 (1997). PMID 9118802.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Dnaconda's recommendation EXR0001j (Japanese text)
            Featured content Dnaconda's recommendation EXR0074e (English text)
            Featured content Dnaconda's recommendation EXR0074j (Japanese text)
            Featured content Promoter Collection (Outline) (English text)
            Featured content Promoter Collection (Outline) (Japanese text)
            Featured content Hedgehog signaling (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB08061_A6Ddp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pGL3_RV6R
            Region: chicken delta-crystallin Basal Pro,8x3'Gli-BS,SV40 pA
            Sequence file: RDB08061_A6Ddd.seq check
            >08061_08061_A6Dd_pGL3-RV6R_D01_10.ab1
                1 NNNNNNNNNN NNNNNNNNNG NCGCCNGGCC TTTCTTTATG TTTTTGGCGT CTTCCNTTTT
               61 ACCAACAGTA CCGGAATGCC AAGCTTGGCT GCCCGCACAG CCCTGCTCAC CGTCTCCGCA
              121 GCTCAGCCCT GGCTGGTCGC TCCGTGCTCT CCGGTCTCAC AGCCCCGCTT TTACGTGCCC
              181 CGGAGCCTGG GCTGGCCCTG CAGGTCGACT CTAGAGGATC CCCGGGAACA GATTCGCGAT
              241 CGAGAGCTTC TACTTGGGTG TTCCCTGCTT GTCGGAGCTT CTACTTGGGT GTTCCCTGCT
              301 TGTCGAGGTC GAGAGCTTCT ACTTGGGTGT TCCCTGCTTG TCGGAGCTTC TACTTGGGTG
              361 TTCCCTGCTT GTCGAGGTCG AGAGCTTCTA CTTGGGTGTT CCCTGCTTGT CGGAGCTTCT
              421 ACTTGGGTGT TCCCTGCTTG TCGAGGTCGA GAGCTTCTAC TTGGGTGTTC CCTGCTTGTC
              481 GGAACTTCTA CTTGGGTGTT CCCTGCTTGT CGAGGTCGAT CGCGAATCTG TTCCCGGGGA
              541 TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA
              601 AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG
              661 CAATAAACAA GTTAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGANGTGTG
              721 GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CNAATGTGAT ATGGCTGATC CAGACATGAT
              781 AAGATACNTT GATGAGTTTG GACNANCCAC AACTANAANT GCAGTGAAAA AAATGCTTTA
              841 TTTGTGNAAT TTGTGATGCT ATTGCTTNAT TTGTAACNNT NNNNNNNNTG CNNTAAACNA
              901 GTTANNNNNN ANNTGCNNTC ATTTTTATGT TTNNNNGTTC NNGGGGNNNN NGNNGGNNNG
            961 NNNTTTTTNN NNNNNNNN
            //
            Primer: RDB08068_F1
            Region: SV40 pA
            Sequence file: RDB08061_A6Dde.seq check
            >08061_08061_A6Dd_RDB8068_F1_C12_03_ABI08.ab1
                1 NNNNNNNNNN NNNNNGNNNG GNNNTCCNNG ANANTTTAAA ATTACCCTTA GAAAGCGGTC
               61 TGTGAAAAAC CCCTACCCAA TTTCCTTTTT GTTAAGTGAC CTAATTAACA GGAGGACACA
              121 GAGGGTGGAT GGGCAGCCTA TGATTGGAAT GTCCTCTCAA GTAGAGGAGG TTAGGGTTTA
              181 TGAGGACACA GAGGAGCTTC CTGGGCCTCG AGGCAGACAT GATAAGATAC ATTGATGAGT
              241 TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG
              301 CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA
              361 TTCATTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC
              421 TCTNNNNNNG TGGTATGGCT GATTATGATC TCTNNNNNNN GNNCTNTNNN TNNNATATTC
              481 CTTATNANNN NCNNNNNNNA NTAAAAAGCT AAANGNNNNC AATTTNNNNN NATANNNATT
            541 AANNNNANAC NCTNNNNNCC TGTGTGGAGT AANANNNNAN NNNNNGNNNN NNNNNN
            //
            Primer: Luc-C_F
            Region: Luc 3',small t intron,SV40 NLS
            Sequence file: RDB08061_A6Ddf.seq check
            >08061_08061_A6Dd_Luc-C_F_B12_02_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNCNG NNNNNAACNA CCGCGNNNNN NTGCGCGGAG GAGTTGTGTT
               61 TGTGGACGAA GTACCGAAAG GTCTTACCGG AAAACTCGAC GCAAGAAAAA TCAGAGAGAT
              121 CCTCATAAAG GCCAAGAAGG GCGGAAAGTC CAAATTGTAA AATGTAACTG TATTCAGCGA
              181 TGACGAAATT CTTAGCTATT GTAATACTCT AGAGGATCTT TGTGAAGGAA CCTTACTTCT
              241 GTGGTGTGAC ATAATTGGAC AAACTACCTA CAGAGATTTA AAGCTCTAAG GTAAATATAA
              301 AATTTTTAAG TGTATAATGT GTTAAACTAC TGATTCTAAT TGTTTGTGTA TTTTAGATTC
              361 CAACCTATGG AACTGATGAA TGGGAGCAGT GGTGGAATGC CTTTAATGAG GAAAACCTGT
              421 TTTGCTCAGA AGAAATGCCA TCTAGTGATG ATGAGGCTAC TGCTGACTCT CAACATTCTA
              481 CTCCTCCAAA AAAGAAGAGA AAGGTAGAAG ACCCCAAGGA CTTTCCTTCA GAATTGCTAA
              541 GTTTTTTGAG TCATGCTGTG TTTAGTAATA GAACTCTTGC TTGCTTTGCT ATTTACACCA
              601 CAAAGGAAAA AGCTGCACTG CTATACAAGA AAATTATGGA AAAATATTCT GTAACCTTTA
              661 TAAGTAGGCA TAACAGTTAT AATCATAACA TACTGTTTTT TCTTACTCCA CACAGGCATA
              721 GAGTGTCTGC TATTAATAAC TATGCTCAAA AATTGTGTAC CTTTAGCTTT TTAANTTGTA
              781 AAGGGGTTAA TAAGGAATAT TTGATGTATA GTGNCTTGAC TAGAGATCAT ANTCAGNNNN
              841 ANCACNNTTG TAGANGGNTT NACTTGCNTN NAAAANNCTC CCACNNCCTC CCCCTGANNC
              901 TGAANCNTNA ANNGAATGCA ATTGNTNGNN GTNNANNTNG TTTANTGCNN NTNNNNNNGG
              961 TTACNANNNN NGCANNNNNN NCNCNANNTT NANNANNANN CNTTTTTTTT CNNTGCNNTN
            1021 NTNNTNGNGG NTNNNCNANC TCNNNNNNNN NN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Sasaki, H., A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development, 124 (7): 1313-1322 (1997). PMID 9118802.
            reference Gunarta, I.K., Critical role of glioma-associated oncogene homolog 1 in maintaining invasive and mesenchymal-like properties of melanoma cells. Cancer Sci. 108 (8): 1602-1611 (2017). PMID 28635133.
            user_report Li, L., Upregulation of amplified in breast cancer 1 contributes to pancreatic ductal adenocarcinoma progression and vulnerability to blockage of hedgehog activation. Theranostics 11 (4): 1672-1689 (2021). PMID 33408774.
            user_report Dettmer, R., FGF2 Inhibits Early Pancreatic Lineage Specification during Differentiation of Human Embryonic Stem Cells. Cells 9 (9): 1927 (2020). PMID 32825270.
            user_report Babu, D., Novel GLI2 mutations identified in patients with Combined Pituitary Hormone Deficiency (CPHD): Evidence for a pathogenic effect by functional characterization. Clin. Endocrinol. (Oxf). 90 (3): 449-456 (2019). PMID 30548673.
            user_report Torrado, B., Ciliary Entry of the Hedgehog Transcriptional Activator Gli2 Is Mediated by the Nuclear Import Machinery but Differs from Nuclear Transport in Being Imp-alpha/beta1-Independent. PLoS One 11 (8): e0162033 (2016). PMID 27579771.
            user_report Sugiyama, M., Paired related homeobox 1 is associated with the invasive properties of glioblastoma cells. Oncol. Rep. 33 (3): 1123-1130 (2015). PMID 25522823.
            user_report Shiohama, T., Oxidative stress-induced JNK1 phosphorylation inhibits hedgehog signalling and osteoblast differentiation. Cell Biol. Int. Rep. 21 (2); 53-62 (2014).
            user_report Wang, D.H., Hedgehog signaling regulates FOXA2 in esophageal embryogenesis and Barrett's metaplasia. J. Clin. Invest. 124 (9): 3767-80 (2014). PMID 25083987.

            2022.05.17

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