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8x3'Gli-BS-delta51-LucII

Reporter plasmid carring eight copies of Gli-binding site

Catalog number RDB08061
Resource name 8x3'Gli-BS-delta51-LucII
Clone info. Reporter plasmid carring eight copies of Gli-binding site.
Use 8xm3'Gli-BS-delta51-LucII (cat.# RDB08062) and delta51-LucII (cat.# RDB08068) as negative control.
Vector backbone delta51-LucII (Plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor Sasaki, Hiroshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Development, 124, 1313-1322 (1997).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08061 8x3'Gli-BS-delta51-LucII DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Dnaconda's recommendation EXR0001j (Japanese text)
Featured content Promoter Collection (Outline) (English text)
Featured content Promoter Collection (Outline) (Japanese text)
Featured content Hedgehog signaling (English text)

References

original Sasaki, H., A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development, 124 (7): 1313-1322 (1997). PMID 9118802.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08061_A6Ddp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL3-RV6R RDB08061_A6Dda.seq
>08061_08061_A6Dd_pGL3-RV6R_D01_10.ab1
    1 GTTCTCCTCA AGAGAAGATG GCGCCGGGCC TTTCTTTATG TTTTTGGCGT CTTCCATTTT
   61 ACCAACAGTA CCGGAATGCC AAGCTTGGCT GCCCGCACAG CCCTGCTCAC CGTCTCCGCA
  121 GCTCAGCCCT GGCTGGTCGC TCCGTGCTCT CCGGTCTCAC AGCCCCGCTT TTACGTGCCC
  181 CGGAGCCTGG GCTGGCCCTG CAGGTCGACT CTAGAGGATC CCCGGGAACA GATTCGCGAT
  241 CGAGAGCTTC TACTTGGGTG TTCCCTGCTT GTCGGAGCTT CTACTTGGGT GTTCCCTGCT
  301 TGTCGAGGTC GAGAGCTTCT ACTTGGGTGT TCCCTGCTTG TCGGAGCTTC TACTTGGGTG
  361 TTCCCTGCTT GTCGAGGTCG AGAGCTTCTA CTTGGGTGTT CCCTGCTTGT CGGAGCTTCT
  421 ACTTGGGTGT TCCCTGCTTG TCGAGGTCGA GAGCTTCTAC TTGGGTGTTC CCTGCTTGTC
  481 GGAACTTCTA CTTGGGTGTT CCCTGCTTGT CGAGGTCGAT CGCGAATCTG TTCCCGGGGA
  541 TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA
  601 AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG
  661 CAATAAACAA GTTAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG
  721 GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGAT ATGGCTGATC CAGACATGAT
  781 AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAAAT GCAGTGAAAA AAATGCTTTA
  841 TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAAGCTG CAATAAACAA
  901 GTTAACAACA AATTGCATTC ATTTTTATGT TTTCAGGTTC AGGGGGGAGG TGTGGGGAGG
  961 GTTTTTTTAA AGCTAGTTAA AACCTTCTAC CAATGTGATA TTGGGCTTGA ATTCCTGCCT
 1021 CGCACGTTTT TCGGTTGAAT GAACGGATGA AAAAACACAT CCTGACAACA TGGCAAACTT
 1081 CCCCTGAGAG AACGGGTCTA CACACTTTGA TCCAGAAAGT CGAGGATGTC CGAGGAACTC
 1141 GACATGACCA CCGATCAAGG GACGGCCTTA CGACAGTGAT GCTGTCTATT GTGCATCGTG
1201 AGCACGTGAC TC
//
Primer: Luc-C_F RDB08061_A6Ddb.seq
>08061_08061_A6Dd_Luc-C_F_B12_02_ABI08.ab1
    1 GAAAAATACG GGTCGGCCAG TCAAGTAACA ACCGCGAAAA AGTTGCGCGG AGGAGTTGTG
   61 TTTGTGGACG AAGTACCGAA AGGTCTTACC GGAAAACTCG ACGCAAGAAA AATCAGAGAG
  121 ATCCTCATAA AGGCCAAGAA GGGCGGAAAG TCCAAATTGT AAAATGTAAC TGTATTCAGC
  181 GATGACGAAA TTCTTAGCTA TTGTAATACT CTAGAGGATC TTTGTGAAGG AACCTTACTT
  241 CTGTGGTGTG ACATAATTGG ACAAACTACC TACAGAGATT TAAAGCTCTA AGGTAAATAT
  301 AAAATTTTTA AGTGTATAAT GTGTTAAACT ACTGATTCTA ATTGTTTGTG TATTTTAGAT
  361 TCCAACCTAT GGAACTGATG AATGGGAGCA GTGGTGGAAT GCCTTTAATG AGGAAAACCT
  421 GTTTTGCTCA GAAGAAATGC CATCTAGTGA TGATGAGGCT ACTGCTGACT CTCAACATTC
  481 TACTCCTCCA AAAAAGAAGA GAAAGGTAGA AGACCCCAAG GACTTTCCTT CAGAATTGCT
  541 AAGTTTTTTG AGTCATGCTG TGTTTAGTAA TAGAACTCTT GCTTGCTTTG CTATTTACAC
  601 CACAAAGGAA AAAGCTGCAC TGCTATACAA GAAAATTATG GAAAAATATT CTGTAACCTT
  661 TATAAGTAGG CATAACAGTT ATAATCATAA CATACTGTTT TTTCTTACTC CACACAGGCA
  721 TAGAGTGTCT GCTATTAATA ACTATGCTCA AAAATTGTGT ACCTTTAGCT TTTTAATTTG
  781 TAAAGGGGTT AATAAGGAAT ATTTGATGTA TAGTGCCTTG ACTAGAGATC ATAATCAGCC
  841 ATACCACATT TGTAGAGGGT TTTACTTGCT TTAAAAAACC TCCCACACCC TCCCCCCTGA
  901 ACCTGAAACA TAAAATGAAT GCAATTGTTT GTTTGTTAAC TTTGTTTATT GCAGCTTATA
  961 TGGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAT AAAGCATTTT TTTCACTGCA
 1021 TTTCTAGTTG TGGTTTGTCA AACTCATTCA TGTATCTTTA TCATGTCCTG GCTCGAAGCC
 1081 CAGAAAGCTC CTCTGTGTCC CTCATAAACC CTAACCTTCC TCTACCTTGA GAGGAACATT
 1141 CCAATCATAG GGCTGTCCAT TCCACCTTCT TGGTCCTCCC TGTTTAATAT AGGGTTCACT
1201 TTAACCAAAA GGAAAATGTG GAGAGGGCGT CTTTC
//
Primer: RDB08068_F1 RDB08061_A6Ddc.seq
>08061_08061_A6Dd_RDB8068_F1_C12_03_ABI08.ab1
    1 CCAGGACCAG ATTGGAAGGG GACTTCCCAG ATATTTTAAA ATTACCCTTA GAAAGCGGTC
   61 TGTGAAAAAC CCCTACCCAA TTTCCTTTTT GTTAAGTGAC CTAATTAACA GGAGGACACA
  121 GAGGGTGGAT GGGCAGCCTA TGATTGGAAT GTCCTCTCAA GTAGAGGAGG TTAGGGTTTA
  181 TGAGGACACA GAGGAGCTTC CTGGGCCTCG AGGCAGACAT GATAAGATAC ATTGATGAGT
  241 TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG
  301 CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA
361 TTCATTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Sasaki, H., A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development, 124 (7): 1313-1322 (1997). PMID 9118802.
reference Gunarta, I.K., Critical role of glioma-associated oncogene homolog 1 in maintaining invasive and mesenchymal-like properties of melanoma cells. Cancer Sci. 108 (8): 1602-1611 (2017). PMID 28635133.
user report Torrado, B., Ciliary Entry of the Hedgehog Transcriptional Activator Gli2 Is Mediated by the Nuclear Import Machinery but Differs from Nuclear Transport in Being Imp-alpha/beta1-Independent. PLoS One 11 (8): e0162033 (2016). PMID 27579771.
user report Sugiyama, M., Paired related homeobox 1 is associated with the invasive properties of glioblastoma cells. Oncol. Rep. 33 (3): 1123-1130 (2015). PMID 25522823.
user report Shiohama, T., Oxidative stress-induced JNK1 phosphorylation inhibits hedgehog signalling and osteoblast differentiation. Cell Biol. Int. Rep. 21 (2); 53-62 (2014).
user report Wang, D.H., Hedgehog signaling regulates FOXA2 in esophageal embryogenesis and Barrett's metaplasia. J. Clin. Invest. 124 (9): 3767-80 (2014). PMID 25083987.

2019.03.07

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